Resting-state functional magnetic resonance imaging reveals the effects of intravesical electrical stimulation on brain activity and functional connectivity in rats with detrusor underactivity

IF 3.7 3区医学 Q2 NEUROSCIENCES

Brain Research Bulletin Pub Date : 2025-10-09 DOI:10.1016/j.brainresbull.2025.111576

Han Deng , Limin Liao , Xing Li , Yixi Liu , Zitian He , Haoyu Sun

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Abstract

Introduction

The central mechanisms of intravesical electrical stimulation (IVES) were explored in a rat model of detrusor underactivity (DU) induced by bilateral pelvic nerve crush (bPNC). Resting-state functional magnetic resonance imaging (rs-fMRI) was used to evaluate regional homogeneity (ReHo) and functional connectivity (FC).

Methods

Eighteen female Sprague-Dawley rats were randomly assigned to sham, bPNC, or IVES groups. The IVES group received 20-minute daily stimulations for 20 days after bPNC. Following treatment, rs-fMRI, cystometry, and immunofluorescence staining for ΔFosB (neuronal activity marker) and GAP43 (axonal plasticity marker) were performed.

Results

Compared with bPNC rats, IVES significantly reduced maximum cystometric capacity and post-void residual volume, and increased detrusor pressure variation during voiding. The bPNC group had higher bladder capacity, increased filling-phase pressure variation, and lower voiding pressure than the sham group. Compared to sham and IVES rats, bPNC rats showed reduced ReHo in the right striatum and periaqueductal gray (PAG). FC between the right striatum and bilateral somatosensory cortex, and between the right PAG and left cerebellum, was weaker in bPNC rats. ΔFosB expression in the right striatum and PAG was higher in bPNC rats than in sham and IVES groups.

Conclusions

IVES may improve DU via modulation of neuronal activity in the right striatum and PAG and enhancing FC between the right striatum and somatosensory cortex, and between the right PAG and left cerebellum.

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静息状态功能磁共振成像揭示了膀胱内电刺激对逼尿肌活动不足大鼠脑活动和功能连通性的影响。

在双侧骨盆神经压迫（bPNC）致逼尿肌活动不足（DU）大鼠模型中，探讨了膀胱内电刺激（IVES）的中枢机制。静息状态功能磁共振成像（rs-fMRI）评价区域均匀性（ReHo）和功能连通性（FC）。方法：雌性Sprague-Dawley大鼠18只，随机分为sham组、bPNC组和IVES组。在bPNC后的20天内，IVES组每天给予20分钟的刺激。治疗后进行rs-fMRI、膀胱术、免疫荧光染色ΔFosB（神经元活性标记物）和GAP43（轴突可塑性标记物）。结果：与bPNC大鼠相比，IVES显著降低了最大膀胱容量和排尿后残余体积，增加了排尿时逼尿肌压力变化。与假手术组相比，bPNC组膀胱容量增大，填充期压力变化增大，排尿压力降低。与sham和IVES大鼠相比，bPNC大鼠右侧纹状体和导水管周围灰质（PAG）的ReHo减少。bPNC大鼠右侧纹状体与双侧体感觉皮层之间、右侧PAG与左侧小脑之间的FC较弱。bPNC大鼠右侧纹状体和PAG中ΔFosB的表达高于sham组和IVES组。结论：IVES可能通过调节右侧纹状体和PAG的神经元活动，增强右侧纹状体与体感觉皮层、右侧PAG与左侧小脑之间的FC来改善DU。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Brain Research Bulletin 医学-神经科学

CiteScore

6.90

自引率

2.60%

发文量

253

审稿时长

67 days

期刊介绍： The Brain Research Bulletin (BRB) aims to publish novel work that advances our knowledge of molecular and cellular mechanisms that underlie neural network properties associated with behavior, cognition and other brain functions during neurodevelopment and in the adult. Although clinical research is out of the Journal''s scope, the BRB also aims to publish translation research that provides insight into biological mechanisms and processes associated with neurodegeneration mechanisms, neurological diseases and neuropsychiatric disorders. The Journal is especially interested in research using novel methodologies, such as optogenetics, multielectrode array recordings and life imaging in wild-type and genetically-modified animal models, with the goal to advance our understanding of how neurons, glia and networks function in vivo.