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Copper nanoclusters with aggregation-induced emission: an effective photodynamic antibacterial agent for treating bacterial-infected wound
IF 6.7 3区 材料科学
Nanoscale Pub Date : 2025-01-25 DOI: 10.1039/d4nr04718d
Zhen Jiang, Yongqi Wei, Yun Wang, Songjie Han, Ze Li, Sihang Liu, Zihao Wang, Zhijun Li, Ting Feng, Haiguang Zhu, Xun Yuan
{"title":"Copper nanoclusters with aggregation-induced emission: an effective photodynamic antibacterial agent for treating bacterial-infected wound","authors":"Zhen Jiang, Yongqi Wei, Yun Wang, Songjie Han, Ze Li, Sihang Liu, Zihao Wang, Zhijun Li, Ting Feng, Haiguang Zhu, Xun Yuan","doi":"10.1039/d4nr04718d","DOIUrl":"https://doi.org/10.1039/d4nr04718d","url":null,"abstract":"Designing antibacterial agents with broad-spectrum antibacterial effects and resistance-free properties is essential for treating bacterial infectious wounds. In this study, we present the design of copper nanoclusters (Cu NCs) that exhibit aggregation-induced emission (AIE). This was achieved by controlling the aggregation state of ligand layers (cysteine and chitosan) through the manipulation of pH and temperature. The AIE properties, characterized by strong photoluminescence (PL), a large Stokes shift, and microsecond-long lifetimes, enable these Cu NCs to generate significant amounts of reactive oxygen species (ROS) upon light illumination for efficient bacterial elimination without inducing drug resistance. As a result, they effectively inactivate various microbial pathogens, including Gram-negative and Gram-positive bacteria, as well as Candida albicans (C. albicans), achieving elimination rates of 99.52% for Escherichia coli (E. coli), 98.89% for Staphylococcus aureus (S. aureus), and 94.60% for C. albicans in vitro. Furthermore, the natural antibacterial properties of chitosan and Cu species enhance the photodynamic antibacterial efficacy of the AIE-typed Cu NCs. Importantly, in vivo experiments demonstrate that these Cu NCs can effectively eradicate bacteria at infection sites, reduce inflammation, and promote collagen synthesis, facilitating nearly 100% wound recovery in S. aureus-infected wounds within 9 days. The findings of this study are of considerable significance, providing a foundation for the application of AIE-typed Cu NCs in photodynamic nanotherapy for bacterial infections.","PeriodicalId":92,"journal":{"name":"Nanoscale","volume":"13 1","pages":""},"PeriodicalIF":6.7,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143030834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Doping Gd16 Nanoclusters for Expanded Optical Properties and Thermometry Application
IF 6.7 3区 材料科学
Nanoscale Pub Date : 2025-01-25 DOI: 10.1039/d4nr04779f
Tingting Li, Jinyu Liu, Feng Jiang, Sheng-Rong He, Jinzhe Liu, Weinan Dong, Ying Zhang, Li Yanan, Zhennan Wu
{"title":"Doping Gd16 Nanoclusters for Expanded Optical Properties and Thermometry Application","authors":"Tingting Li, Jinyu Liu, Feng Jiang, Sheng-Rong He, Jinzhe Liu, Weinan Dong, Ying Zhang, Li Yanan, Zhennan Wu","doi":"10.1039/d4nr04779f","DOIUrl":"https://doi.org/10.1039/d4nr04779f","url":null,"abstract":"Lanthanide metal clusters are composed of rigid multinuclear metal cores encapsulated by organic ligands, which have become one of the most interesting research frontiers because of their fantastic architecture, intriguing physical and chemical properties, and potential applications. However, little attention was paid to exploring their potential as highly efficient optical materials. The Gd<small><sub>16</sub></small> clusters as a new cluster structure that has a rich and varied coordination environment, which is highly conducive to doping and thus controlling luminescence and luminescence color modulation. We achieved green emission by doping Tb<small><sup>3+</sup></small> ions and red emission by doping Eu<small><sup>3+</sup></small> ions in the Gd<small><sub>16</sub></small> cluster structure. Meanwhile, we achieved the red-orange-yellow color-tunable luminescence by controlling the composition of the Tb<small><sup>3+</sup></small> and Eu<small><sup>3+</sup></small> ions. Studies on the PL properties show that the Gd<small><sub>16</sub></small> as the host can be used for doping and efficiently photosensitize the Tb<small><sup>3+</sup></small> ions and Eu<small><sup>3+</sup></small> ions. The existence of energy transfer from ligand to Tb<small><sup>3+</sup></small> ions and Eu<small><sup>3+</sup></small> ions in the co-doped Ln<small><sub>16</sub></small> clusters was sufficiently demonstrated by time-resolved photoluminescence spectroscopies tests, and the energy transfer efficiency in the clusters was calculated. Furtherly, the temperature-dependent photoluminescent properties of these clusters were investigated to determine their potential as luminescent thermometers.","PeriodicalId":92,"journal":{"name":"Nanoscale","volume":"87 1","pages":""},"PeriodicalIF":6.7,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143030832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Liposome: a tool to raise the Cherenkov Radiation yield and to restore fluorophore properties in aqueous media
IF 6.7 3区 材料科学
Nanoscale Pub Date : 2025-01-24 DOI: 10.1039/d4nr02605e
Sébastien Saou, Mathieu Moreau, Anne Berrou, Vivian Lioret, Marta Hernandez-Garcia, Richard A. Decreau
{"title":"Liposome: a tool to raise the Cherenkov Radiation yield and to restore fluorophore properties in aqueous media","authors":"Sébastien Saou, Mathieu Moreau, Anne Berrou, Vivian Lioret, Marta Hernandez-Garcia, Richard A. Decreau","doi":"10.1039/d4nr02605e","DOIUrl":"https://doi.org/10.1039/d4nr02605e","url":null,"abstract":"Liposomes used for the study were prepared and carefully characterized multiple times until all batches indicated the same characterization data (DOPC/cholesterol derivative (1:1.15 mol%), 14 mg(DOPC)/mL, dDLS = 130 nm, 2x1011 liposome/nm3of prepared batch, polydispersity index PDI = 0.1). The study shows that such a liposome suspension raises the yield in Cherenkov Radiation (CR) by 1.6-fold when in presence of [68Ga]-GaCl3, an efficient CR emitter (beta particle energy E = 1800 KeV). Also, liposomes were found to prevent aggregation of a water-soluble phthalocyanine-pyranine PcPy4 dyad upon encapsulation, leading to its spectacular fluorescence restoration. Altogether, upon efficient 68Ga-radiolabelling of NODAGA-chelate immobilized at the liposome surface (99% radiolabelling yield, radio-TLC showed) encapsulating PcPy4 dyad (Caverage = 97 M, Clumen = 300 M), subsequent Cherenkov Radiation Energy Transfer (CRET) at the dyad antenna occurred. Internal energy transfers and fluorescence emission from the emitter dyad led to a 2.6-fold raise in radiance measured in the near-infrared (NIR) window (i.e. ca 400 nm pseudo-Stokes shift). A similar raise in radiance was measured in the green window when encapsulation was achieved with eosin at the same rate. Liposome were found to be stable in PBS over 7 days regardless of the encapsulated fluorophore (no raise in dDLS diameter, no release of encapsulated dyes measured after Sephadex and FPLC repurification sequence), with some decay over 22 hours in a PBS/fetal calf serum mixture (1:1 vol.).","PeriodicalId":92,"journal":{"name":"Nanoscale","volume":"38 1","pages":""},"PeriodicalIF":6.7,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143026571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Changing amyloid nucleation process by small molecule and substrate: a way to build two-dimensional materials
IF 6.7 3区 材料科学
Nanoscale Pub Date : 2025-01-24 DOI: 10.1039/d4nr04624b
Chao Chen, Chenyang Wu, Tiantian Yang, Wenhui Zhao, Jiangtao Lei, Dongdong Lin
{"title":"Changing amyloid nucleation process by small molecule and substrate: a way to build two-dimensional materials","authors":"Chao Chen, Chenyang Wu, Tiantian Yang, Wenhui Zhao, Jiangtao Lei, Dongdong Lin","doi":"10.1039/d4nr04624b","DOIUrl":"https://doi.org/10.1039/d4nr04624b","url":null,"abstract":"The assembly of two-dimensional (2D) materials on substrates presents a wide range of potential applications in nanomaterials. However, the tunable nucleation process in molecular assembly is less information available in the literature. In this paper, a neurodegenerative disease-related short peptide and a small molecule named Fast Green (FG) were selected for their binding affinity with mica / highly oriented pyrolytic graphite (HOPG) substrates. Based on atomic force microscopy (AFM) and molecular dynamics (MD) simulation, we investigated the control of 2D assemblies. With the tuning of FG small molecules and substrates, the assemblies grew epitaxially from nanosheets to nanofilms on mica and highly ordered nanofilaments on HOPG substrates. Notably, the nuclei formed in an orderly array without a critical size or lag phase in the presence of FG molecules on the HOPG substrate, facilitating a quicker co-assembly of ordered filaments compared to bulk conditions. Our MD simulations further demonstrated that the interaction between Aβ16-22 molecules and HOPG substrate was primarily due to π-π interactions between aromatic rings, which led to the formation of single-layer filaments by lying on the surface of HOPG. Additionally, parallel π-π stacking acted as the primary force to inhibit the aggregation of peptides into fibrils. Overall, our results provide a strategy for modulating the interaction of amyloid peptides with small molecules and substrates in the assembly of 2D nanomaterials.","PeriodicalId":92,"journal":{"name":"Nanoscale","volume":"51 1","pages":""},"PeriodicalIF":6.7,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143026568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
High sensitivity prostate-specific antigen SERS detection platform based on laser resonance nanoparticles
IF 6.7 3区 材料科学
Nanoscale Pub Date : 2025-01-24 DOI: 10.1039/d4nr04510f
Shi-Ying Fu, Shan-Shan Xu, Hongmei Li, Xian-Ming Guo, Jia-Sheng Lin, Bing Guan, Bin Chen, Tao Wang, Yue-Jiao Zhang, Jian-Feng Li
{"title":"High sensitivity prostate-specific antigen SERS detection platform based on laser resonance nanoparticles","authors":"Shi-Ying Fu, Shan-Shan Xu, Hongmei Li, Xian-Ming Guo, Jia-Sheng Lin, Bing Guan, Bin Chen, Tao Wang, Yue-Jiao Zhang, Jian-Feng Li","doi":"10.1039/d4nr04510f","DOIUrl":"https://doi.org/10.1039/d4nr04510f","url":null,"abstract":"Accurate quantitative analysis of cancer-related specific biomarkers in clinical serum is very important for the early diagnosis and treatment of cancer. Hospitals often use serum prostate-specific antigen (PSA) as a biomarker associated with prostate cancer diagnosis and prognosis, and prostate cancer cells often produce more PSA than benign cells, leading to elevated PSA levels in the blood. In this study, an immunoassay based on surface-enhanced Raman scattering (SERS) was established for the detection of PSA employing magnetic beads along with SERS nanotags.. The hospital now takes two hours to test the results, and the equipment price is high, the detection price is high, and the penetration rate of township hospitals in China is low. SERS has super-sensitive and fast detection ability, and the detection result in 10 minutes significantly reduces the waiting time. Besides, the detection method is simple, cheap and portable, providing a reference for the popularity of township health centers. To evaluate the clinical applicability of this method, 75 male clinical serum samples were tested, most of which were in the gray area of 4.0-10.0 ng/mL. The experimental results show that our detection method has a good agreement with the results measured by the electrochemical luminescence (ECL) system in the hospital clinical laboratory. Our detection limit for actual samples from patients can reach 0.029 ng/mL. Therefore, our clinical serum PSA marker detection method based on sers has a great market in towns and villages.","PeriodicalId":92,"journal":{"name":"Nanoscale","volume":"17 1","pages":""},"PeriodicalIF":6.7,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143026569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Gadolinium oxide-decorated graphene oxide-based dual-stimuli-responsive smart fluids
IF 6.7 3区 材料科学
Nanoscale Pub Date : 2025-01-24 DOI: 10.1039/d4nr04941a
Hyukjoon Gwon, Hamin Kim, Seungae Lee
{"title":"Gadolinium oxide-decorated graphene oxide-based dual-stimuli-responsive smart fluids","authors":"Hyukjoon Gwon, Hamin Kim, Seungae Lee","doi":"10.1039/d4nr04941a","DOIUrl":"https://doi.org/10.1039/d4nr04941a","url":null,"abstract":"Gadolinium and its compounds have attracted attention for application in various fields owing to their dielectric and magnetic properties; however, using gadolinium compounds has not yet been reported in the field of smart fluids. Herein, gadolinium oxide (Gd<small><sub>2</sub></small>O<small><sub>3</sub></small>)-based composites were used to develop dual-stimuli-responsive smart fluids. The resulting Gd<small><sub>2</sub></small>O<small><sub>3</sub></small> nanoparticle (NP)-decorated graphene oxide (Gd<small><sub>2</sub></small>O<small><sub>3</sub></small>/GO) composites responded to external electric and magnetic fields due to the presence of GO and Gd<small><sub>2</sub></small>O<small><sub>3</sub></small>, respectively. The rheological properties of the electro-magneto-rheological (EMR) fluids under electric and magnetic fields were subsequently investigated. The magnetic response of the EMR fluids was enhanced with increasing Gd<small><sub>2</sub></small>O<small><sub>3</sub></small> content; however, the electric response was reduced. Thus, the Gd<small><sub>2</sub></small>O<small><sub>3</sub></small> content of the composites played an important role in the rheological properties of the EMR fluids. The electro- and magneto-responsive properties of the composite material were tunable owing to the instability of the electrostatic interactions between the composite particles. Moreover, the fabricated EMR fluids exhibited a higher dispersion stability than the GO-based electro-rheological fluid because of the hydrophobic oleic acid coating on the Gd<small><sub>2</sub></small>O<small><sub>3</sub></small> NPs. This study demonstrates the potential of expanding the material selection for developing smart fluid systems.","PeriodicalId":92,"journal":{"name":"Nanoscale","volume":"33 1","pages":""},"PeriodicalIF":6.7,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143026570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Forming N-Heterocyclic Carbene Monolayers: Not All Deposition Methods are the Same
IF 6.7 3区 材料科学
Nanoscale Pub Date : 2025-01-24 DOI: 10.1039/d4nr04428b
Aruna Chandran, Nathaniel Dominique, Gurkiran Kaur, Vincent Clark, Phattananawee Nalaoh, Mark D. Aloisio, Lilian Chinenye Ekowo, Isabel M Jensen, Cathleen Crudden, Netzahualcóyotl Arroyo-Currás, David Jenkins, Jon Camden
{"title":"Forming N-Heterocyclic Carbene Monolayers: Not All Deposition Methods are the Same","authors":"Aruna Chandran, Nathaniel Dominique, Gurkiran Kaur, Vincent Clark, Phattananawee Nalaoh, Mark D. Aloisio, Lilian Chinenye Ekowo, Isabel M Jensen, Cathleen Crudden, Netzahualcóyotl Arroyo-Currás, David Jenkins, Jon Camden","doi":"10.1039/d4nr04428b","DOIUrl":"https://doi.org/10.1039/d4nr04428b","url":null,"abstract":"N-heterocyclic carbenes (NHCs) are unrivaled in their ability to form persistent and tunable monolayers on noble metal surfaces, with disciplines from heterogeneous catalysis and microelectronics fabrication rapidly adopting this technology. It is currently assumed that different NHC monolayer preparation protocols yield equivalent surfaces; however, a direct comparison of the leading synthetic protocols is yet to validate this assumption. Herein, we explore the binding of NHC ligands to gold (Au) surfaces prepared using the five most common NHC deposition methods and discover significant differences in the resulting monolayer composition and structure. In this work, NHC-Au systems are prepared according to literature procedures starting from either the free carbene, the CO<small><sub>2</sub></small> adduct, the bicarbonate salt, or the triflate salt. The resulting surfaces are characterized with surface-enhanced Raman spectroscopy (SERS), laser desorption/ionization mass spectrometry (LDI-MS), electrochemistry, and X-ray photoelectron spectroscopy (XPS). These data indicate that the free carbene, vacuum annealing, and solvent annealing methods form chemisorbed NHC monolayers, as expected; however, the solution phase methods without annealing yield surfaces with a fundamentally different character. Although XPS is widely used to confirm the binding of NHCs to metal surfaces, it does not capture the differences in these deposition procedures and should be used with caution. Taken together, these results reveal a significant variation of the NHC surface structure as a function of deposition procedure and provide a critical benchmark to govern the design and preparation of future NHC monolayer systems.","PeriodicalId":92,"journal":{"name":"Nanoscale","volume":"109 1","pages":""},"PeriodicalIF":6.7,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143027060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Altermagnetism in two-dimensional Ca2RuO4 perovskite 二维Ca2RuO4钙钛矿的电磁特性
IF 6.7 3区 材料科学
Nanoscale Pub Date : 2025-01-23 DOI: 10.1039/d4nr04053h
J. W. González, A. M. León, C. González-Fuentes, R. A. Gallardo
{"title":"Altermagnetism in two-dimensional Ca2RuO4 perovskite","authors":"J. W. González, A. M. León, C. González-Fuentes, R. A. Gallardo","doi":"10.1039/d4nr04053h","DOIUrl":"https://doi.org/10.1039/d4nr04053h","url":null,"abstract":"We propose and characterize a novel two-dimensional material, 2D-CRO, derived from bulk calcium-based ruthenates (CROs) of the Ruddlesden–Popper family, Ca<small><sub><em>n</em>+1</sub></small>Ru<small><sub><em>n</em></sub></small>O<small><sub>3<em>n</em>+1</sub></small> (<em>n</em> = 1 and 2). Using density functional theory, we demonstrate that 2D-CRO maintains structural stability down to the monolayer limit, exhibiting a tight interplay between structural and electronic properties. Notably, 2D-CRO displays altermagnetic behavior, characterized by zero net magnetization and strong spin-dependent phenomena, stabilized through dimensionality reduction. This stability is achieved by breaking inversion symmetry along the <em>z</em>-axis, favoring altermagnetic properties even in the absence of van der Waals interactions. Through theoretical models and computational analysis, we explore the altermagnetic behavior of 2D-CRO, both with and without spin–orbit coupling. We identify the spin components that contribute to the altermagnetic character and highlight the potential of 2D-CRO as a promising material for investigating altermagnetic phenomena and topological features.","PeriodicalId":92,"journal":{"name":"Nanoscale","volume":"74 1","pages":""},"PeriodicalIF":6.7,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143020734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Facet-Controlled Electrosynthesis of Nanoparticles by Combinatorial Screening in Scanning Electrochemical Cell Microscopy 扫描电化学细胞显微镜中组合筛选的面控纳米颗粒电合成
IF 6.7 3区 材料科学
Nanoscale Pub Date : 2025-01-23 DOI: 10.1039/d4nr04564e
Heekwon Lee, Jesús A Muñoz-Castañeda, Hang Ren
{"title":"Facet-Controlled Electrosynthesis of Nanoparticles by Combinatorial Screening in Scanning Electrochemical Cell Microscopy","authors":"Heekwon Lee, Jesús A Muñoz-Castañeda, Hang Ren","doi":"10.1039/d4nr04564e","DOIUrl":"https://doi.org/10.1039/d4nr04564e","url":null,"abstract":"Controlled synthesis of faceted nanoparticles on the surface without explicit use of ligands has gained attention due to their potential applications in electrocatalysis and sensing. Electrodeposition is a desirable method, but controlling the size, spatial distribution, and morphology of the nanoparticles requires extensive optimization. Here, we report spatially resolved synthesis of shape-controlled Pt nanoparticles and fast screening of synthesis conditions in scanning electrochemical cell microscopy (SECCM) using pulse potentials. Screening occurs at individual ~µm2 areas isolated in SECCM, allowing multiple conditions to be screened in one mapping experiment. The screening reveals that the formation of (100) facets in Pt nanoparticles is sensitive to the upper and lower potential limits in the square-wave potential pulse. The facet control is explained by the facet-dependent migration effect from the concurrent hydrogen evolution reaction during Pt deposition. Moreover, the density and size of nanoparticles can also be controlled. This approach paves the way for automated synthesis and characterization of single-facet metallic nanoparticles, offering great potential for advancements in electrocatalysis and sensor applications.","PeriodicalId":92,"journal":{"name":"Nanoscale","volume":"62 1","pages":""},"PeriodicalIF":6.7,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143020737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Nanoscale size impact of nanoparticle interaction and activity studies with urease 纳米颗粒与脲酶相互作用及其活性研究的纳米尺度影响
IF 6.7 3区 材料科学
Nanoscale Pub Date : 2025-01-23 DOI: 10.1039/d4nr04417g
Priya Bhardwaj, Bhawana Bisht, Vijayender Bhalla
{"title":"Nanoscale size impact of nanoparticle interaction and activity studies with urease","authors":"Priya Bhardwaj, Bhawana Bisht, Vijayender Bhalla","doi":"10.1039/d4nr04417g","DOIUrl":"https://doi.org/10.1039/d4nr04417g","url":null,"abstract":"Herein, we provide insights into the size-dependent interactions of silver nanoparticles (AgNPs) with urease and their implications for enzyme inhibition. AgNPs with a size of 5 nm exhibited the strongest binding affinity of 66 nM, resulting in significant enzyme attachment, interfering enzyme conformation, and a consequent loss of activity. Mid-sized AgNPs, <em>i.e.</em>, 20 and 50 nm, exhibited binding affinities of 712 and 616 nM, causing only slight structural alterations. In contrast, 100 nm AgNPs demonstrated a high binding affinity of 171 nM accompanied by a favorable enthalpic contribution and a pronounced inhibitory effect.","PeriodicalId":92,"journal":{"name":"Nanoscale","volume":"27 1","pages":""},"PeriodicalIF":6.7,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143020739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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