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Cross-reactivity of hantavirus antibodies after immunization with PUUV antigens PUUV 抗原免疫后汉坦病毒抗体的交叉反应。
IF 3.2 4区 生物学
Biotechnology and applied biochemistry Pub Date : 2024-05-23 DOI: 10.1002/bab.2604
Layaly Shkair, Diksha Sharma, Shaimaa Hamza, Ekaterina Garanina, Venara Shakirova, Ilsiyar Khaertynova, Maria Markelova, Vera Pavelkina, Albert Rizvanov, Svetlana Khaiboullina, Manoj Baranwal, Ekaterina Martynova
{"title":"Cross-reactivity of hantavirus antibodies after immunization with PUUV antigens","authors":"Layaly Shkair,&nbsp;Diksha Sharma,&nbsp;Shaimaa Hamza,&nbsp;Ekaterina Garanina,&nbsp;Venara Shakirova,&nbsp;Ilsiyar Khaertynova,&nbsp;Maria Markelova,&nbsp;Vera Pavelkina,&nbsp;Albert Rizvanov,&nbsp;Svetlana Khaiboullina,&nbsp;Manoj Baranwal,&nbsp;Ekaterina Martynova","doi":"10.1002/bab.2604","DOIUrl":"10.1002/bab.2604","url":null,"abstract":"<p>Nephropathia epidemica (NE), caused by Puumala (PUUV) orthohantavirus, is endemic in the Republic of Tatarstan (RT). There are limited options for NE prevention in RT. Currently, available vaccines are made using Haantan (HNTV) orthohantavirus antigens. In this study, the efficacy of microvesicles (MVs) loaded with PUUV antigens to induce the humoral immune response in small mammals was analyzed. Additionally, the cross-reactivity of serum from immunized small mammals and NE patients with HNTV, Dobrava, and Andes orthohantaviruses was investigated using nucleocapsid (N) protein peptide libraries. Finally, the selected peptides were analyzed for allergenicity, their ability to induce an autoimmune response, and their interaction with Class II HLA. Several N protein peptides were found to be cross-reactive with serum from MVs immunized small mammals. These cross-reactive epitopes were located in oligomerization perinuclear targeting and Daxx-interacting domains. Most cross-reactive peptides lack allergenic and autoimmune reactivity. Molecular docking revealed two cross-reacting peptides, N6 and N19, to have good binding with three Class II HLA alleles. These peptides could be candidates for developing vaccines and therapeutics for NE.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":"71 5","pages":"1139-1153"},"PeriodicalIF":3.2,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141080665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Puerarin alleviates acrolein-induced atherosclerosis by activating the MYH9-mediated SIRT1/Nrf2 cascade to inhibit the activation of inflammasome 葛根素通过激活 MYH9 介导的 SIRT1/Nrf2 级联抑制炎症小体的活化，从而缓解丙烯醛诱导的动脉粥样硬化。
IF 3.2 4区 生物学
Biotechnology and applied biochemistry Pub Date : 2024-05-23 DOI: 10.1002/bab.2603
XiaoNing Li, YeTing Li, HuiHui Jiao, AiTing Wang, Man Zheng, ChunYan Xiang, FengLei Zhang
{"title":"Puerarin alleviates acrolein-induced atherosclerosis by activating the MYH9-mediated SIRT1/Nrf2 cascade to inhibit the activation of inflammasome","authors":"XiaoNing Li,&nbsp;YeTing Li,&nbsp;HuiHui Jiao,&nbsp;AiTing Wang,&nbsp;Man Zheng,&nbsp;ChunYan Xiang,&nbsp;FengLei Zhang","doi":"10.1002/bab.2603","DOIUrl":"10.1002/bab.2603","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <p>Puerarin (Pue) has significant antioxidant and anti-inflammatory properties. This work was designed to clarify and investigate the potential mechanisms of Pue in atherosclerosis (AS) progression.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <p>In vivo, acrolein (Acr) was inhaled through drinking water to construct AS model. In vitro, CCK-8 assay and lactate dehydrogenase (LDH) assay kit were used to detect cell viability. Apoptosis was detected by flow cytometry. The content of malondialdehyde (MDA) was determined by commercial kit, the level of inflammatory factors was detected by ELISA, and proteins were determined by western blot. Pue administration could effectively reduce blood lipid level in Acr-fed mice. Pue suppressed oxidative stress, the formation of atherosclerotic plaques, and the process of aortic histological changes. Pue pretreatment decreased MDA in HUVECs and maintained the activity of antioxidant enzymes. Pue upregulated SIRT1/Nrf2 cascade in HUVECs. Pue increased MYH9 and inhibited NLRP3 inflammasome-related proteins, and the inhibition of MYH9 significantly impaired Pue-induced Nrf2 activation. Moreover, HUVEC cytotoxicity and apoptosis are alleviated by Pue, in addition to NLRP3-mediated pyroptosis in HUVECs induced by Acr. MYH9 inhibitors effectively suppressed the pyroptosis induced by Acr and prevented injury to HUVECs. In addition, Pue promoted SIRT1/Nrf2 cascade activation in HUVECs. Pue may alleviate Acr-induced AS by activating the MYH9-mediated SIRT1/Nrf2 cascade to inhibit inflammasome activation.</p>\u0000 </section>\u0000 </div>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":"71 5","pages":"1129-1138"},"PeriodicalIF":3.2,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141086501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Correction to “Retraction” 更正 "撤回"。
IF 2.8 4区 生物学
Biotechnology and applied biochemistry Pub Date : 2024-05-22 DOI: 10.1002/bab.2606
{"title":"Correction to “Retraction”","authors":"","doi":"10.1002/bab.2606","DOIUrl":"10.1002/bab.2606","url":null,"abstract":"<p>Retraction Statement: Inhibition of protein phosphatase 5 (PP5) suppresses survival and growth of colorectal cancer cells</p><p>Wang, J., Zhu, J., Dong, M., Yu, H., Dai, X. and Li, K. (2015), Inhibition of protein phosphatase 5 (PP5) suppresses survival and growth of colorectal cancer cells. Biotechnology and Applied Biochemistry, 62: 621−627. https://doi.org/10.1002/bab.1308</p><p>We apologize for errors in the retraction text wording which should have read as follows:</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":"71 3","pages":"691"},"PeriodicalIF":2.8,"publicationDate":"2024-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bab.2606","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141075240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A one-process production of completely biotinylated proteins in a T7 expression system 在 T7 表达系统中一次性生产完全生物素化的蛋白质。
IF 3.2 4区 生物学
Biotechnology and applied biochemistry Pub Date : 2024-05-21 DOI: 10.1002/bab.2598
Takuma Kawashima, Mitsuki Nakamura, Masafumi Sakono
{"title":"A one-process production of completely biotinylated proteins in a T7 expression system","authors":"Takuma Kawashima,&nbsp;Mitsuki Nakamura,&nbsp;Masafumi Sakono","doi":"10.1002/bab.2598","DOIUrl":"10.1002/bab.2598","url":null,"abstract":"<p>Streptavidin is a tetrameric protein with high specificity and affinity for biotin. The interaction between avidin and biotin has become a valuable tool in nanotechnology. In recent years, the site-specific biotin modification of proteins using biotin ligases, such as BirA, has attracted attention. This study established an in vivo method for achieving the complete biotinylation of target proteins using a single plasmid co-expressing BirA and its target proteins. Specifically, a biotin-modified protein was produced in <i>Escherichia coli</i> strain BL21(DE3) using a single plasmid containing genes encoding both BirA and a protein fused to BirA's substrate sequence, Avitag. This approach simplifies the production of biotinylated proteins in <i>E. coli</i> and allows the creation of various biotinylated protein types through gene replacement. Furthermore, the biotin modification rate of the obtained target protein could be evaluated using Native-PAGE without performing complicated isolation operations of biotinylated proteins. In Native-PAGE, biotin-modified proteins and unmodified proteins were confirmed as clearly different bands, and it was possible to easily derive the modification rate from the respective band intensities.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":"71 5","pages":"1070-1078"},"PeriodicalIF":3.2,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141070461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Correction to “Retraction” 更正 "撤回"。
IF 2.8 4区 生物学
Biotechnology and applied biochemistry Pub Date : 2024-05-20 DOI: 10.1002/bab.2610
{"title":"Correction to “Retraction”","authors":"","doi":"10.1002/bab.2610","DOIUrl":"10.1002/bab.2610","url":null,"abstract":"<p>Retraction Statement: Lentivirus-mediated knockdown of Mphase phosphoprotein 8 inhibits proliferation of colon cancer cells</p><p>Liang, X., Liu, T., Zhang, W., Zhang, K., Guo, S. and Liang, J. (2017), Lentivirus-mediated knockdown of M-phase phosphoprotein 8 inhibits proliferation of colon cancer cells. Biotechnology and Applied Biochemistry, 64: 911−917. https://doi.org/10.1002/bab.1504</p><p>We apologize for errors in the retraction text wording which should have read as follows:</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":"71 3","pages":"695"},"PeriodicalIF":2.8,"publicationDate":"2024-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bab.2610","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141070482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Correction to “Retraction” 更正 "撤回"。
IF 2.8 4区 生物学
Biotechnology and applied biochemistry Pub Date : 2024-05-20 DOI: 10.1002/bab.2611
{"title":"Correction to “Retraction”","authors":"","doi":"10.1002/bab.2611","DOIUrl":"10.1002/bab.2611","url":null,"abstract":"<p>Retraction Statement: Long noncoding RNA linc-ITGB1 promotes cell migration and invasion in human breast cancer</p><p>Yan, M., Zhang, L., Li, G., Xiao, S., Dai, J. and Cen, X. (2017), Long noncoding RNA linc-ITGB1 promotes cell migration and invasion in human breast cancer. Biotechnology and Applied Biochemistry, 64: 5−13. https://doi.org/10.1002/bab.1461</p><p>We apologize for errors in the retraction text wording which should have read as follows:</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":"71 3","pages":"696"},"PeriodicalIF":2.8,"publicationDate":"2024-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bab.2611","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141070513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Correction to “Retraction” 更正 "撤回"。
IF 2.8 4区 生物学
Biotechnology and applied biochemistry Pub Date : 2024-05-20 DOI: 10.1002/bab.2608
{"title":"Correction to “Retraction”","authors":"","doi":"10.1002/bab.2608","DOIUrl":"10.1002/bab.2608","url":null,"abstract":"<p>Retraction Statement: Knockdown of GPR137 by RNAi inhibits pancreatic cancer cell growth and induces apoptosis</p><p>Cui, X., Liu, Y., Wang, B., Xian, G., Liu, X., Tian, X. and Qin, C. (2015), Knockdown of <i>GPR137</i> by RNAi inhibits pancreatic cancer cell growth and induces apoptosis. Biotechnology and Applied Biochemistry, 62: 861−867. https://doi.org/10.1002/bab.1326</p><p>We apologize for errors in the retraction text wording which should have read as follows:</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":"71 3","pages":"693"},"PeriodicalIF":2.8,"publicationDate":"2024-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bab.2608","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141070471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Correction to “Retraction” 更正 "撤回"。
IF 2.8 4区 生物学
Biotechnology and applied biochemistry Pub Date : 2024-05-20 DOI: 10.1002/bab.2607
{"title":"Correction to “Retraction”","authors":"","doi":"10.1002/bab.2607","DOIUrl":"10.1002/bab.2607","url":null,"abstract":"<p>Retraction Statement: KLF8 is required for bladder cancer cell proliferation and migration</p><p>Liang, K., Liu, T., Chu, N., Kang, J., Zhang, R., Yu, Y., Li, D. and Lu, D. (2015), KLF8 is required for bladder cancer cell proliferation and migration. Biotechnology and Applied Biochemistry, 62: 628−633. https://doi.org/10.1002/bab.1310</p><p>We apologize for errors in the retraction text wording which should have read as follows:</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":"71 3","pages":"692"},"PeriodicalIF":2.8,"publicationDate":"2024-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bab.2607","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141070467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Correction to “Retraction” 更正 "撤回"。
IF 2.8 4区 生物学
Biotechnology and applied biochemistry Pub Date : 2024-05-20 DOI: 10.1002/bab.2609
{"title":"Correction to “Retraction”","authors":"","doi":"10.1002/bab.2609","DOIUrl":"10.1002/bab.2609","url":null,"abstract":"<p>Retraction Statement: Inhibition of GPR137 suppresses proliferation of medulloblastoma cells in vitro</p><p>Wang, C., Liang, Q., Chen, G., Jing, J. and Wang, S. (2015), Inhibition of <i>GPR137</i> suppresses proliferation of medulloblastoma cells in vitro. Biotechnology and Applied Biochemistry, 62: 868−873. https://doi.org/10.1002/bab.1331</p><p>We apologize for errors in the retraction text wording which should have read as follows:</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":"71 3","pages":"694"},"PeriodicalIF":2.8,"publicationDate":"2024-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bab.2609","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141070472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
ALKBH5 modulates bone cancer pain in a rat model by suppressing NR2B expression ALKBH5 通过抑制 NR2B 的表达调节大鼠模型中的骨癌疼痛。
IF 3.2 4区 生物学
Biotechnology and applied biochemistry Pub Date : 2024-05-19 DOI: 10.1002/bab.2601
Kun Song, Qionghua Cao, Yanping Yang, Yuefen Zuo, Xianping Wu
{"title":"ALKBH5 modulates bone cancer pain in a rat model by suppressing NR2B expression","authors":"Kun Song,&nbsp;Qionghua Cao,&nbsp;Yanping Yang,&nbsp;Yuefen Zuo,&nbsp;Xianping Wu","doi":"10.1002/bab.2601","DOIUrl":"10.1002/bab.2601","url":null,"abstract":"<p>Currently, the clinical treatment of bone cancer pain (BCP) is mainly related to its pathogenesis. The aim of the present study was to elucidate the potential role of <i>N</i>6-methyladenosine (m6A) in BCP in the spinal cord dorsal root ganglia (DRG) of BCP rats and its specific regulatory mechanism in <i>N</i>-methyl-<span>d</span>-aspartate receptor subunit 2B (NR2B). A rat model of BCP was constructed by tibial injection of Walker256 cells, and ALKBH5 and NR2B expression in the spinal cord DRG was detected. ALKBH5 was silenced or overexpressed in PC12 cells to verify the regulatory effect of ALKBH5 on NR2B. The specific mechanism underlying the interaction between ALKBH5 and NR2B was investigated using methylated RNA immunoprecipitation and dual-luciferase reporter gene assays. The results showed increased expression of m6A, decreased expression of ALKBH5, and increased expression of NR2B in the DRG of the BCP rat model. Overexpression of ALKBH5 inhibited NR2B expression, whereas interference with ALKBH5 caused an increase in NR2B expression. In NR2B, interference with ALKBH5 caused an increase in m6A modification, which caused an increase in NR2B. Taken together, ALKBH5 affected the expression of NR2B by influencing the stability of the m6A modification site of central NR2B, revealing that ALKBH5 is a therapeutic target for BCP.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":"71 5","pages":"1105-1115"},"PeriodicalIF":3.2,"publicationDate":"2024-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141064980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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