Biotechnology and applied biochemistry最新文献

{"title":"Novel Sulfonylhydrazones With Sulfonate Ester Framework: Promising Dual Inhibitors of AChE and hCAs.","authors":"Cansu Öztürk, Neslihan Balci, Osman Nuri Aslan, Erbay Kalay","doi":"10.1002/bab.2780","DOIUrl":"https://doi.org/10.1002/bab.2780","url":null,"abstract":"<p><p>Alzheimer's disease (AD) is a progressive neurodegenerative disorder that affects millions of individuals worldwide. Treatment strategies for AD vary depending on cognitive and behavioral symptoms, with cholinergic replacement therapy using acetylcholinesterase (AChE) inhibitors being one of the primary approaches. Recent studies have also identified human carbonic anhydrases (hCAs) as significant therapeutic targets for AD, offering new opportunities for the development of innovative treatments. Carbonic anhydrase inhibitors have been shown to prevent early mitochondrial damage and inhibit H₂O₂ production, thereby reducing amyloid plaque formation. Building on the promising potential of hydrazones particularly sulfonyl hydrazones as anticholinesterase agents, we synthesized 12 novel chlorine-substituted sulfonyl hydrazone compounds containing aryl sulfonate ester groups. These compounds were evaluated for their inhibitory effects on AChE, hCA I, and hCA II enzymes. The synthesized compounds exhibited low nanomolar inhibitory activity, with K_i values ranging from 9.58 ± 2.22 to 104.04 ± 23.82 nM for AChE, 9.12 ± 2.21 to 477.63 ± 218.52 nM for hCA I, and 17.54 ± 7.74 to 564.62 ± 213.98 nM for hCA II. Notably, compound 6 showed strong inhibitory activity against hCA I (K_i = 9.12 ± 2.21 nM; acetazolamide (AZA) = 26.54 ± 3.11 nM) and hCA II (K_i = 17.54 ± 7.74 nM; AZA = 21.73 ± 2.42 nM), whereas compound 4 exhibited superior AChE inhibition (K_i = 9.58 ± 2.22 nM; TAC = 23.12 ± 2.05 nM). The chemical structures of the synthesized compounds were characterized using advanced spectroscopic techniques, including FT-IR, ¹H-NMR, and ¹³C-NMR spectroscopy.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":"e2780"},"PeriodicalIF":3.2,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143982558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum lncRNA PVT1 and Its Targets miR-146a and SIRT1 as Biomarkers for the Early Detection of Breast Cancer and Its Metastasis.","authors":"Noha H Sayed, Mahmoud A Senousy, Olfat G Shaker, Mona A Kortam","doi":"10.1002/bab.2769","DOIUrl":"https://doi.org/10.1002/bab.2769","url":null,"abstract":"<p><p>Early detection of breast cancer (BC) and its metastasis greatly improves the patients' outcomes. The long noncoding RNA plasmacytoma variant translocation 1 (PVT1) and its targets, microRNA-146a and sirtuin 1 (SIRT1), affect BC development; however, their predictive abilities in early detection of BC and its metastatic potential are largely unknown. This study investigated serum PVT1, miR-146a, and SIRT1 as potential diagnostic and predictive biomarkers for BC and its metastasis. 120 BC patients, 40 breast fibroadenoma (BFA) patients, and 80 healthy volunteers were enrolled. An upregulation of serum PVT1 expression and SIRT1 protein levels was observed in BC patients compared to controls and BFA patients. miR-146a was downregulated in BC and BFA patients compared to controls, with lower levels in BFA compared to BC. Metastatic BC patients showcased higher PVT1 and SIRT1 and lower miR-146a levels than the nonmetastatic group. PVT1 and miR-146a were associated with the risk of developing BC among healthy controls. Combining PVT1 and miR-146a in a predictive panel showed substantial diagnostic accuracy for BC (area under the curve [AUC] = 0.91, 95% CI = 0.87-0.95). Only PVT1 was a predictor of the risk of developing BC among BFA cases and the risk of metastasis among BC patients. In BC, PVT1 was negatively correlated with miR-146a and positively correlated with SIRT1 and invasive lobular tumor type. Conclusively, our results highlight the PVT1/miR-146a/SIRT1 trajectory as a potential biomarker for early diagnosis and prognosis of BC. The study introduces the PVT1/miR-146a panel as an excellent biomarker for early BC diagnosis and uncloaks the predictive power of PVT1 in terms of breast tumor malignancy and metastatic tendency.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":"e2769"},"PeriodicalIF":3.2,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143977047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of Bentonite and Charcoal for the Purification of Pectinases From Aspergillus niger ATCC 9642.","authors":"Ana Paula Rossetto, Márcia Santin Trentin, Rosicler Colet, Valéria Borszcz, Ana Luiza Lira, Sabrina Duarte Camargo, Geciane Toniazzo Backes, Jamile Zeni, Eunice Valduga","doi":"10.1002/bab.2778","DOIUrl":"10.1002/bab.2778","url":null,"abstract":"<p><p>The objective of the study was to concentrate and increase the purity of pectinases (exo-polygalacturonase-exo-PG, pectin methylesterase-PME, pectin lyase-PL) obtained from solid-state fermentation by Aspergillus niger ATCC 9642, using activated charcoal and active sodium bentonite. The chemical adsorbents were used at concentrations of 1, 5, 10, 20, 40, and 60 g/L to evaluate the efficiency of purification parameters (purification factor-PF and enzyme recovery-R) of pectinases (exo-PG, PME, and PL). When employing a concentration of 60 g/L of activated charcoal, a PF of 14.15 and 1.91 times and recoveries of 100% and 57% were obtained for exo-PG and PME, respectively. For PL, using a concentration of 40 g/L of activated charcoal, the maximum PF was 2.01 times and the recovery was 138%. With the use of active sodium bentonite (60 g/L), it was possible to obtain a PF of 4.20 times and a recovery of 139% for exo-PG, 2.2 times and 70% for PME, and 0.57 times and 48% for PL, respectively. Activated charcoal, especially at higher concentrations, showed the best results for exo-PG and PME, whereas active sodium bentonite excelled in the purification of exo-PG. The results reinforce the feasibility and efficiency of using chemical adsorbents in the precipitation, concentration, and recovery of pectinases, contributing to the production of enzyme preparations with higher purity and yield for industrial applications.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143954674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lipase: Recombinant Production Methods, Origins, and Industrial Uses.","authors":"Sajad Ehtiati, Seyyed Hossein Khatami","doi":"10.1002/bab.2781","DOIUrl":"https://doi.org/10.1002/bab.2781","url":null,"abstract":"<p><p>Lipases are indispensable enzymes with a wide array of industrial applications. They excel at breaking down triglycerides and are essential in food processing, cleaning agents, biofuel production, environmental remediation, and pharmaceuticals. Microbial sources, particularly bacteria and fungi, dominate lipase production due to their high efficiency, cost-effectiveness, and ability to target specific molecular structures. The advent of genetic engineering has further revolutionized lipase production by enabling the development of tailored enzymes that meet precise industrial needs. As eco-friendly biological catalysts, lipases are pivotal in advancing sustainable and resource-efficient practices, offering significant advantages over traditional chemical methods. Their role in innovation spans increased efficiency, reduced environmental impact, and enhanced specificity across multiple sectors. These qualities establish lipases as vital tools in modern biotechnology, reinforcing their ongoing significance in fostering industrial progress and environmental stewardship.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143976959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oxidation Performance of the Aga2-Laccase Fusion Produced by Yeast Surface Display.","authors":"Dulce Y Arenas-Olivares, Daniel Morales-Guzmán, Karla V Teymennet-Ramírez, Miguel Alcalde, M C Gutiérrez, Fernando Martínez-Morales, María R Trejo-Hernández","doi":"10.1002/bab.2768","DOIUrl":"https://doi.org/10.1002/bab.2768","url":null,"abstract":"<p><p>Laccases are multicopper oxidases with numerous applications because of their wide substrate variety. The yeast surface display (YSD) technology is a powerful tool for the expression of recombinant laccases that can be used to design robust oxidative biocatalysts and combine stability and flexibility in a whole-cell system. In order to gain insights on improving the laccase performance, we investigated the release, from the yeast's surface, of a recombinant laccase (OB1) from Saccharomyces cerevisiae EBY100's cell surface. The laccase, or derivatives thereof, was released as a fusion protein connected, by a flexible peptide linker, to a carrier protein (Aga2 adhesin). The optimum conditions for the release of the fused protein were determined by applying a 2² factorial experimental design, exploring biomass and dithiothreitol concentration as the variables. The released Aga2-3x(G4S)-OB1 derivatives were biochemically characterized and compared to the whole-cell system. Laccase variant TX13A-OB1 was the best-performing fusion protein out of six variants, with a K_m value of 0.0137 ± 0.0032 mM and V_max 0.0075 ± 0.0001 (µmol min^-1) for ABTS. All variants showed thermostability at 60°C and retained over 50% relative activity within a pH range of 3-5, compared to the parental protein. No significant differences in oxidation performance were determined with respect to the oxidation profile of gallic acid, vanillic acid, and catechol. On the other hand, there was an improvement in ferulic acid. Additionally, laccase relative activity remained above 60% in the presence of 10%-20 % ethanol for all variants. This research improved the stability of a recombinant laccase released from the yeast's cell surface carrying an engineered protein domain with adhesin properties, suggesting an influence from this domain on the laccase performance and the potential use of other adhesins for wider applications of laccases and other enzymes.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":"e2768"},"PeriodicalIF":3.2,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143963343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A High-Throughput RP-UPLC Assay for Simultaneous Determination of Telmisartan and Azelnidipine in Pharmaceutical Formulations.","authors":"BhargavaKrishna Pogaku, Ajitha Makula, Mohandass G, Sheeba Santhosh, Harish Gnanasambanthan, Sambit Sarkar, Rama Krishna Reddy Guduru","doi":"10.1002/bab.2765","DOIUrl":"https://doi.org/10.1002/bab.2765","url":null,"abstract":"<p><p>Telmisartan, an angiotensin II receptor blocker, and azelnidipine, a dihydropyridine calcium channel blocker, are often co-prescribed for the effective management of hypertension. The development of accurate and efficient analytical methods is crucial for ensuring the quality control of these combination formulations. This study presents a rapid and reliable reversed-phase ultra-performance liquid chromatography (RP-UPLC) assay for the simultaneous determination of telmisartan and azelnidipine in pharmaceutical formulations. Efficient chromatographic separation was achieved on an ACQUITY BEH C18 column (100 mm × 2.1 mm, 3 µm) using an isocratic mobile phase of pH 4.0 ammonium acetate buffer and acetonitrile (75:25% v/v) at a flow rate of 0.5 mL/min. Detection was performed at 260 nm, with telmisartan and azelnidipine eluting at 1.833 and 3.583 min, respectively. The method demonstrated good efficiency and minimal tailing (<1.5). Validation parameters, including accuracy, precision, linearity, specificity, and sensitivity, were determined according to International Council for Harmonisation guidelines. Calibration curves for both analytes exhibited excellent linearity (correlation coefficients > 0.999) over a concentration range of 50-150%. Recoveries from tablet dosage forms ranged from 98.0% to 102.0%, with assay values falling within the prescribed range. This validated that reversed-phase ultra-performance liquid chromatography assay offers a high-throughput approach suitable for routine quality control analysis of telmisartan and azelnidipine in pharmaceutical formulations.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":"e2765"},"PeriodicalIF":3.2,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143974448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Silico Molecular Docking of 2-Hydroxyanthraquinone-Substituted Spiro-/Ansa Cyclotriphosphazenes: Targeting Apoptosis via Heat Shock Protein Modulation in Breast and Colon Cancer Cells.","authors":"Seda Mesci, Burak Yazgan, Gizem Demir Demirel, Tuba Yildirim, Gönül Yenilmez Çiftçi","doi":"10.1002/bab.2767","DOIUrl":"https://doi.org/10.1002/bab.2767","url":null,"abstract":"<p><p>Cancer research takes a long time and is complex. Preclinical studies prove that compounds can be potential anticancer agents and contribute to cancer studies. Overexpression of survivin may cause decreased sensitivity of anticancer agents and antiapoptotic activation through its excretion from cells via MDR. Anthraquinones and phosphazene compounds, which are among the active biological compounds and identified in many studies on cancer, come to the fore in biochemical, microbiological, and pharmacological studies. In this study, it was aimed to investigate the effect of 2-hydroxyanthraquinone-substituted spiro-/ansa cyclotriphosphazene compounds (II-VIII) on multidrug resistance, ER stress, and apoptotic cell death pathways in breast and colon cancer cells. mRNA expressions of multidrug resistance transporter, ER stress, heat shock, and apoptotic genes assessed by qPCR. Besides protein levels of apoptosis, cell cycle and related signaling pathways (CASP3, BCL-w, sTNF-R, cIAP-2, TRAILRs, IGFBPs, Survivin, XIAP, etc.) were determined by antibody membrane array method in MCF-7 and DLD-1 cell lines. To elucidate the activities of Survivin protein-related compounds, in silico-mediated molecular docking studies were evaluated. ABCs, HSPs, and GRPs gene expressions in MCF-7 and DLD-1 cells were decreased by these compounds. Besides, in gene regulations of apoptosis and signaling pathways, it was observed that the compounds induce overexpression of BAX and underexpression BCL-2. In addition, especially survivin expression was downregulated by all the compounds. It has been determined that the compounds eliminate multidrug resistance in breast and colon cancer cells, suppress HSPs and GRPs genes, and lead the cells to death, especially through the antiapoptotic pathway Survivin. These compounds can be evaluated and developed as Survivin inhibitor agents in anticancer studies.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":"e2767"},"PeriodicalIF":3.2,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143970847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GDF10 Regulates Iron-Dependent Lipid Oxidation in Colorectal Cancer Cells Through Interaction With IGF2.","authors":"Kaobin Ouyang, Tianying Huang, Dan Xie, Hailin Xiong","doi":"10.1002/bab.2762","DOIUrl":"https://doi.org/10.1002/bab.2762","url":null,"abstract":"<p><strong>Background: </strong>This study aims to identify genes with a high likelihood of genetic variation, significant expression differences, and prognostic implications in colorectal cancer (CRC) patients. The research also seeks to investigate the role and mechanisms of key genes in CRC through the analysis of health information data and a combination of internal and external experiments.</p><p><strong>Methods: </strong>The sequence information of CRC patients was obtained from the Cancer Genome Atlas Program (TCGA) database. Key genes were identified through differential expression analysis, enrichment analysis, interaction analysis, and survival curve analysis. Subsequently, Growth and Differentiation Factor 10 (GDF10) overexpression and knockout cell models were developed to investigate the impact of GDF10 on CRC cells using assays such as CCK8, flow cytometry, Transwell, and subcutaneous tumor formation in nude mice. Mechanistically, the effects of GDF10 and IGF2 genes on autophagy, apoptosis, and ferroptosis were studied by introducing different death pathway inhibitors. Changes in reactive oxygen species (ROS), GSH, and MDA levels in cells following silencing were also assessed. Furthermore, the influence of GDF10 and its interacting protein IGF2 on ferroptosis was evaluated through ELISA and fluorescence staining.</p><p><strong>Results: </strong>The analysis of patients in the TCGA database revealed that GDF10 expression was low in microsatellite stable (MSS) type and high in microsatellite instability (MSI) type. It was found to interact with IGF2, with low expression associated with a better prognosis. Overexpression of GDF10 was shown to significantly enhance the proliferation and invasion abilities of CRC cells, whereas low expression promoted cell apoptosis. Within the pathways of autophagy, apoptosis, and ferroptosis, low expression of GDF10 primarily regulated ferroptosis in cells. This regulation involved promoting the iron-dependent lipid oxidation process by mediating binding with IGF2, leading to increased concentrations of iron ions and oxidative metabolites in cells. This process also reduced the formation of lipid droplets and inhibited tumor development.</p><p><strong>Conclusion: </strong>GDF10 plays a crucial role in regulating ferroptosis in CRC cells through mediating IGF2 interaction, suggesting it as a promising therapeutic target for CRC.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":"e2762"},"PeriodicalIF":3.2,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143976834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selection and Identification of Two Electrogenic Bacteria and Their Performance in Wastewater Treatment.","authors":"Wen-Ting Cui, Sha-Sha Zhang, Meng Li, Qiang Zhou, Kai-Jie Cao, Pan Hu, Bo Zhang","doi":"10.1002/bab.2764","DOIUrl":"https://doi.org/10.1002/bab.2764","url":null,"abstract":"<p><p>The potential applications of microbial fuel cells (MFCs) in wastewater treatment and energy recovery have been investigated. MFCs harness the metabolic activity of microorganisms to generate electricity from organic compounds, which is highly important for preventing environmental pollution and constructing an ecological civilization. To address the safety limitations of pathogenic electrogenic bacteria in MFCs, this study isolated two novel nonpathogenic strains, Cellulosimicrobium 99-1 and Listeria innocua 5-2, from livestock samples. Their synergistic interaction boosted voltage output by 8.2% (exceeding 400 mV) compared to pure cultures while achieving 90.6% chemical oxygen demand (COD) and 74% total nitrogen removal in domestic wastewater, demonstrating dual advantages in biosecurity and treatment efficiency. This study provides experimental data and theoretical support for the application of MFC technology in environmental engineering, laying a foundation for further optimizing MFC design and improving wastewater treatment efficiency.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":"e2764"},"PeriodicalIF":3.2,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143975850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploration of Basidiomycetes for Anthraquinone Dyes Decolorization in Textile Wastewater.","authors":"Pragnya Paramita Sahoo, Vikas Kumar, Preeti Pallavi, Adyasha Anapurba Sahoo, Sudip Kumar Sen, Sangeeta Raut","doi":"10.1002/bab.2763","DOIUrl":"https://doi.org/10.1002/bab.2763","url":null,"abstract":"<p><p>Anthraquinone (AQ) dyes are utilized extensively in the textile industry due to their ability to fasten fabrics. The intricate and rigid structures of AQ dyes, however, prevent them from biodegradation. They also create nitrate residues, which persist as effluents in textile wastewater and harm aquatic vegetation by obstructing light from entering the water, which affects both flora and fauna. The use of bioremediation technique is most popular because it is environmentally beneficial and economical. The aim of this study was to isolate white rot fungi (WRF) for their ability to decolorize AQ dyes and their mixtures. The current study shows the decolorization of mixture of AQ dyes (MAQD), namely, Acid blue 129 (AB129), Alizarin cyanin green (ACG), and Remazol brilliant blue R (RBBR) (200 ppm) under optimized parameters: pH 7, temperature 30°C, and shaking speed 80 rpm in 24 h by using suspended fungal isolates, VS12 (93.71%) and WF2 (92.76%) isolated from decaying wood. The highest manganese peroxidase (MnP) activity (2391.77 U/mL) was found in VS12 followed by WF2 (2318.28 U/mL) in 24 h. Moreover, the study revealed that MnP is one of the causes for decolorization of MAQD, as decolorization is directly proportional to MnP activity. On the basis of morphological features and a complete sequence analysis of 18S rRNA gene and internal transcribed spacer (ITS) region, the isolates were identified as Trametes cubensis WF2 and Polyporus umbellatus VS12. This is the first report of white rot fungal isolates T. cubensis WF2 and P. umbellatus VS12 used in efficient decolorization of MAQD (AB129, ACG, RBBR).</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":"e2763"},"PeriodicalIF":3.2,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143977133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}