Biotechnology and applied biochemistry最新文献

{"title":"Bromophenol Derivatives as Multifunctional Antidiabetic Agents: Enzyme Inhibition Profiles and Induced-Fit Docking Analysis.","authors":"Serpil Gerni","doi":"10.1002/bab.70156","DOIUrl":"10.1002/bab.70156","url":null,"abstract":"<p><p>Bromophenol derivatives were evaluated for their inhibitory effects on three key enzymes associated with diabetes: aldose reductase (AR), α-glycosidase, and α-amylase. In vitro enzyme inhibition assays revealed that Compound 6 exhibited the strongest α-glycosidase inhibition (IC₅₀ = 11.948 nM), while Compound 5 showed the highest potency against AR (IC₅₀ = 1.386 µM) and α-amylase (IC₅₀ = 5.588 nM). Selected compounds (5, 6, 7, and 10) displayed low cytotoxicity against normal human fibroblast cells, indicating favorable safety profiles. Complementary induced-fit molecular docking analyses supported the experimental results, highlighting specific interactions between the compounds and the enzyme active sites. These findings demonstrate that bromophenol derivatives possess significant enzyme-inhibitory potential and may serve as promising multifunctional agents for diabetes management. Induced-fit docking studies revealed that Compounds 6, 10, 5, and 7 exhibited the most favorable binding energies across the tested enzymes, consistent with their high inhibitory potential. Compounds 6 and 10 showed strong α-glucosidase binding through key interactions such as π-π stacking with TRP539/PHE575 and TRP406, as well as extensive hydrogen and halogen bonding with catalytic residues including TYR299, ASP203, ASP327, and ARG526. Similarly, Compounds 5 and 7 formed stable docking poses within α-amylase and aldose reductase via multiple hydrogen bonds, halogen contacts, and π-π stacking with crucial residues like ASP197, GLH233, ASN160, TRP20, and TRP219, highlighting their strong affinity and target-specific interactions.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":"2079-2091"},"PeriodicalIF":2.5,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13446434/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147502992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocidal Activity of Barium and Iron-Co-Doped Titanium Dioxide Nanocomposites, Synthesized by Psidium guajava-Mediated Precipitation Method.","authors":"Saumya Goyal, Suhas Ballal, Ankit Punia, Deeplata Sharma, Manish Nagpal, Jaivik Pathak, Indumathi Thangavelu, Srinivas Tadepalli","doi":"10.1002/bab.70155","DOIUrl":"10.1002/bab.70155","url":null,"abstract":"<p><p>The emergence of drug-resistant microorganisms and the need for effective anticancer agents necessitate the development of novel nanomaterials with enhanced biomedical performance. This study aims to synthesize barium and iron dual-doped titanium dioxide (TiBaFeO NC) using a green precipitation method with Psidium guajava leaf extract, targeting improved antimicrobial and anticancer efficacy. The synthesized nanocomposite was characterized by various analytical techniques. XRD confirmed the crystalline anatase phase of TiO₂ and TiBaFeO NC, with average crystallite sizes of 40 and 37 nm, respectively, suitable for biomedical applications. UV-Vis analysis showed a decrease in bandgap from 3.79 eV for TiO₂ to 3.67 eV for TiBaFeO NC, indicating enhanced reactive oxygen species (ROS) generation potential. PL spectra exhibited green emissions at 520 nm for TiO₂ and 523 nm for TiBaFeO NC, reflecting a higher oxygen vacancy defect density in the doped nanocomposite. Biological evaluations demonstrated that TiBaFeO NC exhibited superior antimicrobial activity against Gram-positive bacteria (Staphylococcus aureus, Bacillus subtilis, Bacillus megaterium), Gram-negative bacteria (Shigella dysenteriae, Escherichia coli, Proteus vulgaris), and fungi (Candida albicans). Furthermore, TiBaFeO NC showed enhanced anticancer activity against human breast cancer cells (MDA-MB-231) with an IC₅₀ of 9.8 µg/mL, outperforming TiO₂. These results suggest that TiBaFeO NC is a promising nanocomposite for advanced biomedical applications, combining enhanced antimicrobial and anticancer properties through defect-mediated ROS generation.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":"2068-2078"},"PeriodicalIF":2.5,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13446443/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147627246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic Potential of Ginger Rhizomes (Zingiber officinale) on Leukemia.","authors":"Abdolhossein Hajizadeh, Parmida Nafei, Kimiya Seraj, Faranak Zargari, Nassim Rastgar, Farshad Zare, Obid Khamidov, Ghadeer Mohammed Ali Basha, Mustafa Sattar, Mehdi Amirhooshangi, Nima Ghavamikia, Payam Ali-Khiavi","doi":"10.1002/bab.70130","DOIUrl":"10.1002/bab.70130","url":null,"abstract":"<p><p>Leukemia continues to provide a significant therapeutic challenge due to relapse, medication resistance, and treatment-associated toxicity, which frequently hinder sustained disease management. Rhizomes of ginger (Zingiber officinale) possess bioactive phenolics, notably 6-gingerol and 6-shogaol derivatives, which have demonstrated antileukemia efficacy in preclinical models. This study rigorously assesses the evidence regarding ginger-derived preparations and isolated compounds in both acute and chronic leukemia models, focusing on recurring mechanisms and translational viability. In leukemia cell line investigations and sparse resistant-model data, ginger-related interventions are consistently linked to diminished viability and the induction of mitochondrial apoptosis, typically indicated by alterations in Bax/Bcl-2 ratios, PARP breakage, and caspase-related measurements. Numerous studies indicate redox modulation, often characterized by elevated intracellular reactive oxygen species in leukemic cells, coupled with diminished pro-survival signaling, such as PI3K/Akt, as indicated by decreased pAkt and survivin levels. The suggested immunomodulatory and anti-inflammatory effects, encompassing alterations in NK-cell activity and cytokines like TNF-α and IL-6, are inadequately substantiated within leukemia-specific immunological contexts. Interpretation is limited by the variability in extract composition and chemical characterisation, inconsistent dose and exposure circumstances, dependence on endpoint markers without causative manipulation, and a lack of leukemia-specific clinical data. Ginger-derived compounds exhibit multi-target biological activity that necessitates further exploration through standardized and chemically defined preparations, pharmacokinetic and pharmacodynamic characterization, clinically relevant exposure benchmarks, and meticulously designed leukemia-focused translational and early-phase clinical studies to elucidate safety, efficacy, and compatibility with current therapies.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":"2102-2113"},"PeriodicalIF":2.5,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13446437/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146084332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of Fish Skin (Labeo rohita) and Chia Seed Oil (Salvia hispanica) Blend on the Protein-Rich Breakfast Cereal Product Made Through the Use of Whole and Modified Chia Flour and Fish Skin Protein Isolates.","authors":"Nabanita Ghosh, Monalisha Roy, Dipak Kumar Bhattacharyya","doi":"10.1002/bab.70196","DOIUrl":"https://doi.org/10.1002/bab.70196","url":null,"abstract":"<p><p>The term \"Ready-to-Eat Breakfast Cereal\" refers especially to the processed grain formulations suitable for human consumption without requiring further preparation to save time. It is easy to store, relatively shelf-stable, and lightweight. A significant proportion of them are made with corn, wheat, oats, and rice, with added flavors and fortifications. In this work, an attempt was made to utilize the rich antioxidative blend of fish skin oil (FSO) and chia seed oil (CO). Four formulations were prepared using four different chia seed flour, namely, raw chia seed flour breakfast cereal (RCB), defatted chia seed flour breakfast cereal (DCB), raw roasted chia flour breakfast cereal (RRCB), and roasted defatted chia flour breakfast cereal (RDCB). All the physicochemical tests were satisfactory for each formulation. Among the four formulations, RRCB is liked mostly in hedonic rating test for sensory evaluation by the panel members. Breakfast cereals are sold as milled grains of different cereals that required further cooking or no cooking prior to consumption. Breakfast plays an important role in everyday diet plan as it is the first food that we took after the dinner of previous day. So, breakfast meal should be rich in all nutrients to fulfill the needs till the lunch time. Nowadays, due to busy schedules, attention is moved toward ready-to-eat breakfast items rather than the traditional one.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148629493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RETRACTION: A New Molecularly Imprinted Polymer Electrochemical Sensor Based on CuCo₂O₄/N-Doped CNTs/P-Doped GO Nanocomposite for Detection of 25-Hydroxyvitamin D3 in Serum Samples.","authors":"","doi":"10.1002/bab.70195","DOIUrl":"https://doi.org/10.1002/bab.70195","url":null,"abstract":"","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148590477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustainable Production of Phenolic Compounds From Brewers' Spent Grain: Optimization of Operational Extraction Conditions.","authors":"Cintia Anabela Mazzucotelli, María Celeste Pellegrini, Alejandra Graciela Ponce, Viviana Paola Cyras","doi":"10.1002/bab.70191","DOIUrl":"https://doi.org/10.1002/bab.70191","url":null,"abstract":"<p><p>This study aimed to evaluate the effect of different extraction conditions on the recovery of phenolic compounds from brewers' spent grain (BSG) and their antioxidant capacity. A Plackett-Burman design (PBD) was first applied to identify the key variables influencing the extraction process, followed by response surface methodology (RSM) to optimize the conditions for maximizing phenolic compound yield and antioxidant activity. The optimized extraction parameters included a drying temperature of 50°C, a liquid/solid ratio of 56.5 mL/g, and an extraction temperature of 69.7°C. Experimental validation confirmed that the predicted values for total phenolic content (2.68 mg AGE/g dm) and antioxidant capacities (1.20 mg TE/g dm for DPPH and 5.81 mg TE/g dm for ABTS) were in agreement with experimental data within a 95% confidence level, demonstrating the reliability of the models used. Despite the high phenolic content and antioxidant activity, the extracts showed no antimicrobial effect against Escherichia coli O157:H7 or Listeria innocua. These findings highlight the potential of BSG as a source of antioxidants, reinforcing its possible applications in the food, packaging, pharmaceutical, and cosmetic industries as a natural additive. Additionally, optimizing the extraction process contributes to the valorization of this agro-industrial by-product, promoting sustainability and circular economy practices.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148577145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biotechnological Advances of Lactic Acid Bacteria in Maghrebian Countries: An Updated Review.","authors":"Slim Smaoui, Noureddine Bouras, Teresa D'Amore, Pankaj Koirala, Nilesh Prakash Nirmal, Lotfi Mellouli, Michael D Holtz, Hajer Ben Hlima, Amin Mousavi Khaneghah","doi":"10.1002/bab.70190","DOIUrl":"https://doi.org/10.1002/bab.70190","url":null,"abstract":"<p><p>Due to their technological and functional properties, lactic acid bacteria (LAB) contribute to improving the nutritional value and extending the shelf life of food products. However, the heterogeneity, biodiversity, and complex metabolic pathways of LAB species have required significant scientific efforts to isolate, characterize, and optimize their applications in food technology. Furthermore, variations in prevalence and composition across regions and countries pose additional challenges, driven by the intrinsic capacity of these bacteria to evolve into mutant strains in response to environmental conditions. Within this intricate framework, this study provides an in-depth overview of LAB prevalence and biodiversity in Maghrebian countries, highlighting recent findings on their metabolic pathways. In addition, the molecular and physiological features of newly isolated LAB species originating from the Maghreb are assessed. This review is of particular significance as it provides an updated and region-focused synthesis of recent biotechnological advances in LAB in Maghrebian countries. It highlights their broader implications for sustainable food systems, functional food innovation, and the valorization of local microbial resources.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148577204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Apis mellifera L. for Eco-Friendly Silver Nanoparticles: Green Synthesis, Antioxidant and Antimicrobial Activities","authors":"Merve Keskin,&nbsp;Şaban Keskin","doi":"10.1002/bab.70075","DOIUrl":"10.1002/bab.70075","url":null,"abstract":"<div>\u0000 \u0000 <p>Honeybees (<i>Apis mellifera</i> L.) are social insects that have importance in both pollination and human health with their hive products. Due to their natural life cycle, worker bees die after a few weeks and are thrown out of the hive. However, dead bees with their bioactive compounds, such as carbohydrates and proteins, could be a precursor for green synthesis of silver nanoparticles. Green nanoparticles obtained through green synthesis are used in a wide range of fields, from the environment to medicine, with their unique properties. In this study, the potential for the use of dead bees in the green synthesis of silver nanoparticles was determined. For this purpose, naturally dead bees that were thrown out of the hive within one day were collected, dried, grounded, extracted, and used in green synthesis of silver nanoparticles. The obtained nanoparticles (MS-AgNPs) were characterized, and it was observed that the MS-AgNPs gave maximum absorbance at 465 nm and their sizes varied between 79 and 91 nm. It was clear that they had good antioxidant and antimicrobial activity. It could be concluded that dead bees could be an excellent biological reducing and capping agent for green synthesis of silver nanoparticles.</p>\u0000 </div>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":"73 3","pages":"1036-1044"},"PeriodicalIF":2.7,"publicationDate":"2026-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145343539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Suppression of the Coffee-Ring Effect for OMVs Distribution of TEM Sampling via GA-Induced Marangoni Flow and Inhibition of Radially Capillary Flow","authors":"Maobin Xie,&nbsp;Dingshan Zhang,&nbsp;Jiayu Liu,&nbsp;Yao Jiang,&nbsp;Jin Bai,&nbsp;Hai Qiao","doi":"10.1002/bab.70074","DOIUrl":"10.1002/bab.70074","url":null,"abstract":"<div>\u0000 \u0000 <p>The aggregation of suspended particles caused by the coffee-ring effect (CRE) disrupts uniform distribution and limits accurate transmission electron microscopy (TEM) observation. Here, we show that glutaraldehyde (GA) suppresses CRE in aqueous polystyrene microsphere droplets and enables more uniform outer membrane vesicles (OMVs) deposition for TEM. Specifically, the addition of GA (0%–15% v/v) to 1% w/v, 1 µm red polystyrene microsphere suspensions progressively reduced ring formation: The ratio of maximum-to-minimum gray values decreased from 2.40 ± 0.22 (0% GA) to 1.11 ± 0.06 (15% GA), and mean CRE width dropped from ∼97 to ∼3.2 µm. Microsphere radial velocity decreased from 4.25 ± 0.37 (0% GA) to 0.89 ± 0.08 µm/s (15% GA), whereas bulk solution viscosity increased concomitantly (from ∼4 to ∼232 mPa s at 15% GA), indicating viscosity-mediated suppression of radial capillary flow. Addition of sodium dodecyl sulfate (SDS) partly restored CRE, consistent with reduction of surface-tension gradients that drive Marangoni reflux. Applying GA (6% v/v) to OMVs TEM preparation produced homogeneous OMVs counts across center, middle, and edge regions, without observable morphological alteration. We conclude that GA suppresses CRE primarily by (i) increasing viscosity to inhibit radial capillary flow and (ii) establishing surface-tension-driven Marangoni backflow that redistributes particles away from the contact line. GA thus provides a practical, fixation-compatible approach to improve TEM sample uniformity for biological nanoassemblies.</p>\u0000 </div>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":"73 3","pages":"1023-1035"},"PeriodicalIF":2.7,"publicationDate":"2026-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145421311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Single Particles of Amyloid Beta and α-Synuclein With Seeded Amplification for the Diagnosis of Alzheimer's and Parkinson's Disease","authors":"Alexandra Dybala,&nbsp;Marlene Pils,&nbsp;Oliver Bannach,&nbsp;Gültekin Tamgüney,&nbsp;Detlev Riesner","doi":"10.1002/bab.70083","DOIUrl":"10.1002/bab.70083","url":null,"abstract":"<p>Neurodegenerative diseases, including Alzheimer's disease (AD) and Parkinson's disease (PD), are characterized by the pathological aggregation of specific proteins such as amyloid beta (Aβ) and α-synuclein, respectively. Early detection of these protein aggregates in biological fluids could facilitate timely diagnosis and therapeutic intervention. This study explores an aggregate amplification approach using the surface-based fluorescence intensity distribution analysis (sFIDA) method to enhance the detection sensitivity of Aβ and α-synuclein seeds. Two amplification strategies were investigated: surface-bound and solution-phase amplification. In the case of Aβ, surface-bound amplification using immobilized Aβ-specific antibodies was tested with synthetic Aβ_1-42 seeds and monomeric Aβ_1-42 as the substrate. Despite observable aggregation, self-aggregation of the monomeric substrate interfered with seed-dependent amplification, rendering the approach ineffective at physiologically relevant concentrations. Attempts to suppress self-aggregation using blocking peptides, bovine serum albumin, and truncated Aβ_11-42 substrates were unsuccessful. Solution-phase amplification followed by surface detection also failed to reliably differentiate seeded aggregation from self-aggregation, indicating that Aβ amplification is unsuitable for diagnostic applications. In contrast, α-synuclein exhibited significantly lower self-aggregation, allowing for more effective seeded amplification. Surface-bound α-synuclein amplification successfully detected synthetic seeds at nanomolar concentrations, while solution-phase amplification further improved sensitivity, enabling detection down to the picomolar range. The method was successfully applied to brain homogenates from transgenic PD model mice, demonstrating the potential for detecting α-synuclein seeds in biological samples. These findings highlight the limitations of Aβ amplification for diagnostic purposes while supporting the feasibility of α-synuclein amplification for PD detection. Future work will focus on optimizing this approach for clinical applications.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":"73 3","pages":"1115-1124"},"PeriodicalIF":2.7,"publicationDate":"2026-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bab.70083","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145421244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}