Biotechnology and applied biochemistry最新文献

Lipase-Catalyzed Promiscuous Reaction for the Synthesis of Pyrido[2,3-d]pyrimidine Scaffolds via One Pot Knoevenagel-Michael-Aromatization in Aqueous Ethanol. 脂肪酶催化一锅knoevenagel - michael芳构化合成吡啶[2,3-d]嘧啶支架

IF 2.7 4区生物学

Biotechnology and applied biochemistry Pub Date : 2025-10-05 DOI: 10.1002/bab.70057

Pratik P Wagh, Kiran S Dalal, Suraj P Vasave, Yogesh B Wagh, Bhushan L Chaudhari, Dipak S Dalal

{"title":"Lipase-Catalyzed Promiscuous Reaction for the Synthesis of Pyrido[2,3-d]pyrimidine Scaffolds via One Pot Knoevenagel-Michael-Aromatization in Aqueous Ethanol.","authors":"Pratik P Wagh, Kiran S Dalal, Suraj P Vasave, Yogesh B Wagh, Bhushan L Chaudhari, Dipak S Dalal","doi":"10.1002/bab.70057","DOIUrl":"https://doi.org/10.1002/bab.70057","url":null,"abstract":"Porcine pancreas lipase (PPL) has been used as an efficient green biocatalyst for the multicomponent synthesis of pyrido[2,3-d]pyrimidine derivatives and expanding the biocatalytic promiscuity of lipase in organic synthesis. This robust procedure involves the condensation of various aromatic aldehydes, malononitrile, and 6-amino-1,3-dimethyl uracil in a 1:1:1 molar ratio at 50°C, catalyzed by PPL in an equimolar mixture of water and ethanol (1:1, v/v). The influence of the reaction conditions, including enzyme origin, organic solvents, temperature, and the amount of biocatalyst on the reaction course, was examined. Notably, PPL displayed remarkable catalytic activity, enabling the synthesis of diverse pyrido[2,3-d]pyrimidine derivatives with good to excellent yields (77%-94%). A promiscuous lipase-catalyzed carbon-nitrogen bond formation is presented, accommodating a variety of aromatic aldehydes. This method exhibits several key advantages, including the use of environment-friendly solvents, a nontoxic biocatalyst, mild reaction conditions, straightforward workup procedures, and excellent product yields.","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145231549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biosurfactant Production From Agro-Industrial and Food Waste: A Sustainable Approach to Waste Management. 从农业、工业和食品垃圾中生产生物表面活性剂：一种可持续的废物管理方法。

IF 2.7 4区生物学

Biotechnology and applied biochemistry Pub Date : 2025-09-30 DOI: 10.1002/bab.70058

Pallavi Gupta, Prachi Gaur, Rachana Singh

{"title":"Biosurfactant Production From Agro-Industrial and Food Waste: A Sustainable Approach to Waste Management.","authors":"Pallavi Gupta, Prachi Gaur, Rachana Singh","doi":"10.1002/bab.70058","DOIUrl":"https://doi.org/10.1002/bab.70058","url":null,"abstract":"The global pursuit of sustainable and climate-resilient technologies has accelerated interest in waste valorization for biosurfactant production. Biosurfactants, recognized as eco-friendly alternatives to synthetic surfactants, offer versatile applications across industries due to their biodegradability, low toxicity, and surface-active properties. Despite having number of applications and advantages, commercialization remains difficult primarily due to the high cost, with substrates alone contributing over 50% of the total production cost. Leveraging waste as a feedstock for production has opened new avenues by reducing production costs by 10%-30% while simultaneously promoting environmental sustainability. Waste-derived biosurfactants not only offer significant cost reductions and yield (1 g/L-60 g/L depending on feedstock and strain) and environmental advantages over conventional substrates but also advance circular bioeconomy principles by transforming waste into valuable products and aiding in waste management. This review synthesizes current advancements in biosurfactant classification, microbial sources, and fermentation strategies, with a particular emphasis on the valorization of low-cost waste feedstocks, such as food processing byproducts, lignocellulosic agricultural residues, and industrial effluents. The review further elaborates emerging biotechnological approaches aimed at improving yield, functionality, and process scalability. Major emphasis is on the utilization of waste to address both environmental challenges and economic feasibility in biosurfactant synthesis over conventional substrate-derived biosurfactant.","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145198413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction of an Osteoarthritis Diagnostic Model Based on Hub Immune Cells and Genes by Machine Learning Method. 基于中枢免疫细胞和基因的骨关节炎诊断模型的机器学习构建。

IF 2.7 4区生物学

Biotechnology and applied biochemistry Pub Date : 2025-09-27 DOI: 10.1002/bab.70052

Rong Jiang, Xiaoyu Peng, Kai Zhao

{"title":"Construction of an Osteoarthritis Diagnostic Model Based on Hub Immune Cells and Genes by Machine Learning Method.","authors":"Rong Jiang, Xiaoyu Peng, Kai Zhao","doi":"10.1002/bab.70052","DOIUrl":"https://doi.org/10.1002/bab.70052","url":null,"abstract":"The objective of this investigation was to develop a diagnostic model for osteoarthritis (OA) by integrating immune cell profiling with transcriptomic signatures. Four gene expression datasets related to OA were downloaded from the GEO database. CIBERSORT was employed to evaluate the proportion of different immune cell types. Hub immune cells were selected using three distinct optimization algorithms (LASSO, RFE, and RF). Differentially expressed genes (DEGs) between OA and control samples were screened using the limma package. Subsequently, function analysis and protein-protein interaction (PPI) analysis were conducted. Topology analysis based on four algorithms was performed, and hub genes were identified by overlapping the results of these four algorithms. A diagnostic model was constructed and validated using the ROC curve method. Pearson correlation coefficients between hub immune cell populations and candidate genes were computed using the cor() function in R. Seven types of differentially abundant immune cells were identified between the two groups. After analysis with the RF, LASSO, and RFE algorithms, five overlapping immune cells, namely, T cell CD4 memory resting, NK cell activated, T cell CD4 naive, mast cell resting, and mast cell activated, were selected as hub immune cells. A total of 578 DEGs were selected, which were implicated in the MAPK signaling pathway, focal adhesion, and osteoclast differentiation. Following PPI analysis, five hub genes (CXCL8, EEF1A1, IL1B, EEF2, and IL6) were obtained. The diagnostic model demonstrated excellent performance. Significant correlations were observed between the hub genes and immune cell populations. Through systematic analysis, we identified five key immune cell types and five hub genes associated with immune infiltration in OA. These biomarkers were subsequently utilized to construct a diagnostic prediction model.","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145181843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Network Pharmacology and Experimental Evaluation of Lonicera japonica Flos in Rheumatoid Arthritis. 金银花对类风湿关节炎的网络药理作用及实验评价。

IF 2.7 4区生物学

Biotechnology and applied biochemistry Pub Date : 2025-09-23 DOI: 10.1002/bab.70051

Zihan Yu, Yonghui Yin, Xuan Wang, Guangjin Zhou, Yanren Shang, Yongqing Zhang, Jinghong Hu

{"title":"Network Pharmacology and Experimental Evaluation of Lonicera japonica Flos in Rheumatoid Arthritis.","authors":"Zihan Yu, Yonghui Yin, Xuan Wang, Guangjin Zhou, Yanren Shang, Yongqing Zhang, Jinghong Hu","doi":"10.1002/bab.70051","DOIUrl":"https://doi.org/10.1002/bab.70051","url":null,"abstract":"Lonicera japonica Flos (LJF) possesses antiviral, antioxidant, and anti-inflammatory properties, but its mechanisms against rheumatoid arthritis (RA) are not well understood. This study aims to clarify LJF's molecular mechanisms in treating RA. Active components and targets of LJF and RA were determined using databases such as TCMSP, GeneCards, and OMIM. Protein-protein interaction (PPI) network analysis and pathway enrichment analysis (via KEGG as well as GO databases) were conducted to predict potential targets and pathways. Molecular docking identified potential targets of three specific LJF components. The anti-arthritic effects of LJF were evaluated in vivo using a collagen-induced arthritis (CIA) rat model. Seventeen active LJF components and 126 potential anti-RA targets were identified. Key compounds include quercetin, luteolin, kaempferol, beta-carotene, and beta-sitosterol. Main targets are AKT1, TP53, JUN, TNF, IL6, CASP3, EGFR, RELA, IL1B, and VEGFA. KEGG analysis suggested the involvement of the PI3K-AKT, TNF, and IL-17 pathways. Molecular docking demonstrated that β-sitosterol, quercetin, and luteolin effectively bind to AKT1, IL6, JUN, TNF, and TP53. In vivo studies confirmed that LJF reduces pathological damage and inflammatory markers (TNF-α and IL-6) in a dose-dependent manner, supporting its anti-RA effects via AKT and RELA through PI3K-AKT and NF-κB pathways. This research identified the PI3K-AKT and NF-κB signaling pathways as key targets of LJF, highlighting their roles in its anti-RA effects. These findings point to the possibility of advancing research and clinical use of LJF in RA treatment.","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145129965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome Editing Using the Endogenous Type I-E CRISPR-Cas System in Lactobacillus paracasei ATCC334. 利用内源性I-E型CRISPR-Cas系统对副干酪乳杆菌ATCC334进行基因组编辑

IF 2.7 4区生物学

Biotechnology and applied biochemistry Pub Date : 2025-09-23 DOI: 10.1002/bab.70056

Ni Zuo, Fangyuan Zuo, Yanqiang Liu, Bin Xiang

{"title":"Genome Editing Using the Endogenous Type I-E CRISPR-Cas System in Lactobacillus paracasei ATCC334.","authors":"Ni Zuo, Fangyuan Zuo, Yanqiang Liu, Bin Xiang","doi":"10.1002/bab.70056","DOIUrl":"https://doi.org/10.1002/bab.70056","url":null,"abstract":"Lactobacillus paracasei ATCC334 is a well-known beneficial strain that plays a crucial role in food industry and promotion of human health. However, despite its significance, our understanding of its gene functions remains limited due to obstacles in gene editing techniques. This gap hinders the full utilization and development of this beneficial bacterium. In this study, we targeted L. paracasei ATCC334 as editing chassis. Initially, bioinformatics tools were used to explore a type I-E endogenous clustered regularly interspaced short palindromic repeats (CRISPR)-Cas system within L. paracasei ATCC334. We further analyzed its repeat sequences, spacer sequences, and leader sequence predicted the protospacer adjacent motif (PAM) recognized by this system. To validate our findings, we assessed the accuracy of potential PAM, evaluated the cutting activity of the endogenous CRISPR-Cas system, and studied the impact of the artificial mini-CRISPR array through plasmid interference and genome interference experiments. These results helped us to achieve successful gene knockout and gene integration. Finally, we engineered a strain capable of nicotine degradation. Our study provides valuable insights for the broader development and application of lactobacilli.","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145130037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enzymatic Properties and Structural Insights Into the Derhamnosylating Alkaline α-l-Rhamnosidase From Aspergillus flavus. 黄曲霉脱毛鼠李糖基碱性α-l-鼠李糖苷酶的酶学性质和结构研究。

IF 2.7 4区生物学

Biotechnology and applied biochemistry Pub Date : 2025-09-21 DOI: 10.1002/bab.70053

Kunwar Vishal, Soumen Barman, Divyanshu S Senger, Vinita Yadav, Pramod K Yadav

{"title":"Enzymatic Properties and Structural Insights Into the Derhamnosylating Alkaline α-l-Rhamnosidase From Aspergillus flavus.","authors":"Kunwar Vishal, Soumen Barman, Divyanshu S Senger, Vinita Yadav, Pramod K Yadav","doi":"10.1002/bab.70053","DOIUrl":"https://doi.org/10.1002/bab.70053","url":null,"abstract":"α-l-Rhamnosidases are ubiquitous enzymes responsible for derhamnosylation of α-l-rhamnose moiety from a variety of glycoconjugates and numerous natural glycosides. An α-l-rhamnosidase-secreting fungal strain was isolated from soil sample. Further, it was identified as Aspergillus flavus through internal transcribed spacer (ITS) gene sequencing. The enzyme was purified to homogeneity using ion-exchange and gel filtration chromatography and exhibited molecular weight of 71 ± 1 kDa. The maximum catalytic efficiency for the α-l-rhamnosidase was established to be pH 10.0 and at a temperature of 50°C. The purified enzyme exhibits a Km 0.41 ± 0.06 mM and a Vmax 2.43 ± 0.17 µmol/min/mg for naringin hydrolysis. In this study, we modeled the 3D structure of A. flavus α-l-rhamnosidase using SWISS Model and validated it via PDBsum and PROCHECK. Molecular docking of A. flavus α-l-rhamnosidase with naringin and p-nitrophenyl-α-l-rhamnopyranoside (pNPR) identified key binding interactions. Electrostatic surface analysis highlighted ligand-binding sites, revealing crucial residues for substrate recognition and enzyme stability. Active site residues of A. flavus α-l-rhamnosidase forming hydrogen bonds and hydrophobic interactions with naringin and pNPR were identified, providing insights into substrate specificity and its potential applications in glycoside hydrolysis.","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145111982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of 2-Aminothiazoles as α-Glucosidase Inhibitors: DFT, Molecular Docking, and Antioxidant Studies. 2-氨基噻唑作为α-葡萄糖苷酶抑制剂的评价：DFT、分子对接和抗氧化研究。

IF 2.7 4区生物学

Biotechnology and applied biochemistry Pub Date : 2025-09-16 DOI: 10.1002/bab.70054

Arzu Öztürk Kesebir, Yeliz Demir, Rüya Sağlamtaş, Aykut Öztekin

{"title":"Evaluation of 2-Aminothiazoles as α-Glucosidase Inhibitors: DFT, Molecular Docking, and Antioxidant Studies.","authors":"Arzu Öztürk Kesebir, Yeliz Demir, Rüya Sağlamtaş, Aykut Öztekin","doi":"10.1002/bab.70054","DOIUrl":"https://doi.org/10.1002/bab.70054","url":null,"abstract":"This study investigates the inhibitory potential of 2-aminothiazole derivatives on α-glucosidase (α-Glu) activity and their antioxidant properties using a combination of in vitro and in silico methods. Diabetes mellitus, characterized by chronic hyperglycemia, necessitates effective enzyme inhibitors to manage postprandial glucose levels. Among the studied compounds, structural variations significantly influenced α-Glu inhibition, with 2-amino-4-(4-bromophenyl) thiazole showing the highest potency (Ki: 56.61 ± 1.31 µM). Molecular docking analyses revealed critical interactions within the enzyme's active site, emphasizing the importance of electron-withdrawing groups for enhancing inhibitory activity. Antioxidant properties were assessed using Fe3⁺, Cu2⁺, and ABTS radical scavenging assays, where specific derivatives, particularly compound 5 demonstrated strong radical scavenging activity (ABTS IC50 = 8.5-9 µg/mL) and the highest TPTZ-Fe3⁺ reducing capacity among the derivatives (λ593 = 0.637 ± 0.005). Density functional theory (DFT) analysis further elucidated the electronic properties of these derivatives, identifying low HOMO-LUMO energy gaps as a determinant of reactivity. These findings underscore the therapeutic potential of 2-aminothiazoles as α-Glu inhibitors and antioxidants, paving the way for developing novel treatments for diabetes and oxidative stress-related disorders. This research contributes to the rational design of bioactive molecules with enhanced efficacy and reduced side effects.","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145074351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive Analysis of Gene Expression and Biomarkers in Sepsis Using Bioinformatics, Network Pharmacology and Molecular Modeling Approaches. 利用生物信息学、网络药理学和分子模型方法综合分析败血症的基因表达和生物标志物。

IF 2.7 4区生物学

Biotechnology and applied biochemistry Pub Date : 2025-09-09 DOI: 10.1002/bab.70049

Zhengyun Tian, Weiwei Wang, Hao Hao, Li Kong, Guochen Li

{"title":"Comprehensive Analysis of Gene Expression and Biomarkers in Sepsis Using Bioinformatics, Network Pharmacology and Molecular Modeling Approaches.","authors":"Zhengyun Tian, Weiwei Wang, Hao Hao, Li Kong, Guochen Li","doi":"10.1002/bab.70049","DOIUrl":"https://doi.org/10.1002/bab.70049","url":null,"abstract":"Background: Differentially expressed genes (DEGs) have been known to provide important information on disease mechanisms and potential therapeutic targets. The traditional Chinese medicine (TCM) offers a large reservoir of bioactive compounds that could modulate at these targets. This study is an attempt to investigate the biomarkers in Sepsis and COVID-19 using gene expression analysis and molecular modeling validation of TCM-derived candidate compounds targeting key DEGs associated with sepsis.Methods: Gene expression data were obtained from NCBI, and limma package in R Studio was used to identify DEGs. Functional annotation was followed by gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment. Protein-protein interaction (PPI) networks were created using STRING, and key hub proteins identified utilizing Cytoscape. Molecular docking was conducted using 216 bioactive compounds obtained from TCM databases against target proteins. To study binding stability, molecular dynamics (MD) simulations of 100 ns were performed using GROMACS on top ranked protein-ligand complexes.Results: A total of 432 key DEGs were functionally enriched in disease related pathways. Bioinformatics analysis identified the RRM2, AURKB, and CDK1 as hub proteins that could serve as promising therapeutic agents. Salvianolic Acid C, Hesperidin, and Gallocatechin Gallate were lead TCM compounds which showed strong binding affinity to these targets on the basis of molecular docking. Selected protein-ligand complexes were stable according to MD simulations.Conclusion: The current study indicates the possibility of TCM compounds to target DEGs crucial in sepsis pathology. The integrated bioinformatics approach establishes an approach to identify novel drug candidates, which need further experimental validation.","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145028973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ribonuclease-Based Immunotoxins as Anticancer Agents. 核糖核酸酶免疫毒素作为抗癌药物。

IF 2.7 4区生物学

Biotechnology and applied biochemistry Pub Date : 2025-09-09 DOI: 10.1002/bab.70046

Arezoo Mesri, Negar Asadi, Hadi Maleki-Kakelar, Amir Maleksabet, Ramin Saadatian Kharajo, Mortaza Taheri-Anganeh, Saeid Latifi-Navid

{"title":"Ribonuclease-Based Immunotoxins as Anticancer Agents.","authors":"Arezoo Mesri, Negar Asadi, Hadi Maleki-Kakelar, Amir Maleksabet, Ramin Saadatian Kharajo, Mortaza Taheri-Anganeh, Saeid Latifi-Navid","doi":"10.1002/bab.70046","DOIUrl":"https://doi.org/10.1002/bab.70046","url":null,"abstract":"Ribonucleases (RNases) represent a distinct category of nucleases that facilitate RNA degradation into smaller components. These enzymes are particularly adept at dismantling RNA strands and other materials. A promising strategy for the targeted treatment of cancer cells involves the administration of antibody-based toxic agents designed to eliminate tumor cells specifically. These poisonous agents may include synthetic small-molecule drugs or cytotoxic proteins known as immunotoxins (ITs). ITs are defined by their dual structure, comprising a receptor-targeting element and a cytotoxic component, which may be derived from RNase sourced from plants, bacteria, fungi, or humans. When RNases are used as IT, they can trigger cell cycle arrest or interfere with vital cellular pathways, ultimately leading to apoptosis or the specific destruction of cancer cells. Consequently, this review highlights the application of various RNases in cancer treatment, underscoring their cytotoxic properties, which are crucial for advancing research on health and therapeutic interventions.","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145028935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Central Composite Design Optimization for the Synthesis of Butyl Acetate Catalyzed by Liquid Lipase. 液体脂肪酶催化合成乙酸丁酯的中心复合设计优化。

IF 2.7 4区生物学

Biotechnology and applied biochemistry Pub Date : 2025-09-07 DOI: 10.1002/bab.70050

Meng-Yuan Jiang, Zi-Teng Yu, Mei-Ting Zhang, An-Qi Li, Wei Liu, Hui-Xiong Zhong, Meng-Ying Wu, Ke-Ke Cheng

{"title":"Central Composite Design Optimization for the Synthesis of Butyl Acetate Catalyzed by Liquid Lipase.","authors":"Meng-Yuan Jiang, Zi-Teng Yu, Mei-Ting Zhang, An-Qi Li, Wei Liu, Hui-Xiong Zhong, Meng-Ying Wu, Ke-Ke Cheng","doi":"10.1002/bab.70050","DOIUrl":"https://doi.org/10.1002/bab.70050","url":null,"abstract":"Butyl acetate, a valuable flavor ester, is conventionally synthesized through acid-catalyzed reactions, which suffer from environmental concerns and inefficiencies. This study explores a greener alternative using liquid lipase Novozym 400238 for its enzymatic synthesis. The central composite design (CCD) within response surface methodology (RSM) was employed to assess the reaction parameters, including temperature, substrate molar ratio, enzyme concentration, and hexane content, along with their effects on the conversion rate. Following model optimization, the optimal reaction conditions were identified as follows: a temperature of 45°C, a molar ratio of n-butanol to acetate ion of 4:1, an enzyme concentration of 8.3%, and a hexane content of 60%. Under these conditions, the esterification reaction lasted for 5 h and achieved a yield exceeding 90%. Furthermore, the liquid lipase exhibited high reusability, maintaining over 80% yield for 11 cycles under optimal conditions. These findings showcase the potential of liquid lipase as a cost-effective and sustainable catalyst for butyl acetate synthesis, offering a promising avenue for green and eco-friendly production processes.","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145013823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0