Biotechnology and applied biochemistry最新文献

{"title":"Research Progress on Biosynthesis and Regulation of Conjugated Linoleic Acid in Lactic Acid Bacteria.","authors":"Xin-Xin Liu, Selar Izzat, Guang-Qiang Wang, Zhi-Qiang Xiong, Lian-Zhong Ai","doi":"10.1002/bab.2737","DOIUrl":"https://doi.org/10.1002/bab.2737","url":null,"abstract":"<p><p>Conjugated linoleic acid (CLA) has gained considerable attention and has been extensively studied owing to its physiological functions, including weight loss, immune regulation, and anti-carcinogenesis. However, the CLA content is very low in natural sources and insufficient to exert a probiotic effect. Additionally, its chemical synthesis is limited by the lack of isomer selectivity, harsh reaction conditions, and the introduction of organic solvents. Therefore, the synthesis of CLA by microorganisms, especially lactic acid bacteria, holds great promise because of its isomer selectivity, green process, and probiotic effects of lactic acid bacteria. This article reviews the CLA biosynthesis abilities and mechanisms of CLA producers, particularly lactic acid bacteria. In addition, the factors that impact the CLA yield are discussed. Special attention has been paid to the regulation of CLA synthesis, including the optimization of culture media and conditions, co-culturing of different strains, and regulation of the activity and expression of CLA-related enzymes. Furthermore, prospective future research directions for CLA biosynthesis regulation are discussed.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143499458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plasma Exosomal circRNAs as Potential Biomarkers for Patients with Septic Shock-Induced Acute Kidney Injury.","authors":"Hailing Yang, Yan Li, Kuo Qu, Chunmei Zhang, Mei Ding, Yang Liu","doi":"10.1002/bab.2736","DOIUrl":"https://doi.org/10.1002/bab.2736","url":null,"abstract":"<p><p>Sepsis-induced acute kidney injury (AKI) is marked by high mortality and poor prognosis. Recent studies suggest that exosomal circRNAs regulate key pathways involved in inflammation, oxidative stress, and apoptosis, processes central to sepsis-induced AKI. This study investigates the differential expression of exosomal circRNAs and their functional implications in the pathogenesis of sepsis-induced AKI. Patients with sepsis-induced AKI and healthy volunteers were recruited for the research. Whole blood samples were collected, and exosomes were isolated and characterized through particle detection, transmission electron microscopy, and Western blot for exosomal marker proteins. Real-time PCR was used to analyze exosomal circRNAs, including circ-Fryl, circ-TLK1, circ-PTK2, circ-Ttc3, circ-VMA21, and circ_0091702. Additionally, assays were performed to assess cell proliferation, apoptosis, and uptake in HK-2 cells exposed to exosomes from both control and sepsis-induced AKI samples. Seven patients with sepsis-induced AKI and six healthy individuals were enrolled. Exosomal protein markers were analyzed from plasma samples of both groups. Exosomes from the sepsis-induced AKI group (mean particle diameter: 146.1 nm) and the control group (mean particle diameter: 150.9 nm) exhibited a characteristic spherical morphology. Plasma levels of circ-Ttc3 (p < 0.05), circ-Fryl (p < 0.01), and circ_0091702 (p < 0.01) were notably lower in the sepsis-induced AKI group relative to the control group. Additionally, HK-2 cells exposed to sepsis-induced AKI exosomes showed upregulation of circ-Ttc3 and downregulation of circ-TLK1. Following exposure to sepsis-induced AKI exosomes, HK-2 cell viability decreased while apoptosis increased. circRNAs such as circ-Ttc3, circ-TLK1, circ-Fryl, and circ_0091702 hold potential as candidate biomarkers and therapeutic targets for sepsis-induced AKI.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143490769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Prediction Nomogram for Moderate-to-Severe Bronchopulmonary Dysplasia in Chinese Very Preterm Infants With Respiratory Distress: A Multi‑Center Prospective Study.","authors":"Chenhong Wang, Zheng Chen, Guannan Bai, Lizhong Du, Yuan Shi, Liping Shi","doi":"10.1002/bab.2718","DOIUrl":"https://doi.org/10.1002/bab.2718","url":null,"abstract":"<p><p>Moderate-to-severe bronchopulmonary dysplasia (msBPD) is a prevalent and severe condition in very preterm infants, contributing to long-term respiratory issues. Developing a predictive model specific to the Chinese population could support early intervention. This research focused on determining risk factors and developing a predictive model for msBPD in very preterm infants with respiratory distress, following the 2018 National Institute of Child Health and Human Development criteria. A prospective multicenter study was conducted across 26 tertiary neonatal intensive care units (NICUs) in China from March 2020 to March 2022. Infants born at < 32 weeks' gestation, admitted within 72 h of birth with a respiratory score ≥ 5, were enrolled. Infants were assigned at random to either a training cohort (800 infants from 18 NICUs) or an external validation cohort (397 infants from 8 NICUs). Predictive factors were identified using multivariate logistic regression and Lasso regression, leading to the development of a prediction nomogram. Key independent predictors included birth weight, surfactant use, early pulmonary hypertension, duration of invasive mechanical ventilation, and necrotizing enterocolitis (Stages II-III). The model demonstrated high accuracy in the training cohort (AUC = 0.844) with good calibration (Hosmer-Lemeshow test, p = 0.247). Independent validation showed consistent discrimination (AUC = 0.849) and calibration (Hosmer-Lemeshow test, p = 0.333), while decision curve analysis confirmed clinical benefit. The developed nomogram-based model offers reliable early msBPD risk assessment in very preterm infants, supporting timely clinical interventions within Chinese NICUs.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143482190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"trans-Ferulic Acid Antagonizes the Anti-Inflammatory Activity of Etoricoxib: Possible Interaction of COX-1 and NOS.","authors":"Md Shimul Bhuia, Raihan Chowdhury, Rubel Hasan, Md Sakib Al Hasan, Siddique Akber Ansari, Irfan Aamer Ansari, Mohammad S Mubarak, Henrique D M Coutinho, Carolina Bandeira Domiciano, Muhammad Torequl Islam","doi":"10.1002/bab.2739","DOIUrl":"https://doi.org/10.1002/bab.2739","url":null,"abstract":"<p><p>This study emphasizes to investigate the modulatory activity of trans-ferulic acid (TFA) on anti-inflammatory activity of etoricoxib (ETO) and underlying mechanisms via formalin-induced licking and paw edema model and in silico study. Inflammation was induced by injecting formalin (50 µL) into the right hind paw of mice. The animals were treated with different doses of TFA (25, 50, and 75 mg/kg, p.o.). The vehicle and ETO (35 mg/kg, p.o.) were provided as positive and negative control, respectively. ETO also served combined with TFA to evaluate the modulatory activity. The licking behavior was counted for the early and late phases, whereas the paw edema diameter was measured by using a slide caliper. All treatment was continued for 7 days until the edema was totally minimized to determine the inflammation's recovery capability for a specific group. Different computed and web tools were used to estimate molecular binding affinity, binding interactions, and pharmacokinetics. The findings demonstrated that TFA significantly (p < 0.05) enhanced the onset of licking and reduced the number of licks compared to vehicle group. TFA also showed a significant (p < 0.05) diminished in paw edema and complete recovered of the edema after 5 days of treatment indicating the anti-inflammatory effects. However, TFA with ETO notably diminished the anti-inflammatory effects of ETO by enhancing paw edema diameter and licking number. TFA also expressed elevated binding affinity of -7.5 and -6.5 kcal/mol toward nitric oxide (NO) synthase and COX-1, respectively. In conclusion, TFA exerted anti-inflammatory effects and reduces anti-inflammatory capability of ETO.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143476284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetically Engineered Bacteria as a Promising Therapeutic Strategy Against Cancer: A Comprehensive Review.","authors":"Zahra Zahedifard, Shirin Mahmoodi, Abdolmajid Ghasemian","doi":"10.1002/bab.2738","DOIUrl":"https://doi.org/10.1002/bab.2738","url":null,"abstract":"<p><p>As a significant cause of global mortality, the cancer has also economic impacts. In the era of cancer therapy, mitigating side effects and costs and overcoming drug resistance is crucial. Microbial species can grow inside the tumor microenvironment and inhibit cancer growth through direct killing of tumor cells and immunoregulatory effects. Although microbiota or their products have demonstrated anticancer effects, the possibility of acting as pathogens and exerting side effects in certain individuals is a risk. Hence, several genetically modified/engineered bacteria (GEB) have been developed to this aim with ability of diagnosing and selective targeting and destruction of cancers. Additionally, GEB are expected to be considerably more efficient, safer, more permeable, less costly, and less invasive theranostic approaches compared to wild types. Potential GEB strains such as Escherichia coli (Nissle 1917, and MG1655), Salmonella typhimurium YB1 SL7207 (aroA gene deletion), VNP20009 (∆msbB/∆purI) and ΔppGpp (P_Tet and P_BAD), and Listeria monocytogenes Lm^at-LLO have been developed to combat cancer cells. When used in tandem with conventional treatments, GEB substantially improve the efficacy of anticancer therapy outcomes. In addition, public acceptance, optimal timing (s), duration (s), dose (s), and strains identification, interactions with other strains and the host cells, efficacy, safety and quality, and potential risks and ethical dilemmas include major challenges.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143476281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SSB-2548 Inhibits CXCR-4 Activation, Inducing Apoptosis in Acute Myeloid Leukemia Cells.","authors":"Ayed A Dera","doi":"10.1002/bab.2726","DOIUrl":"https://doi.org/10.1002/bab.2726","url":null,"abstract":"<p><p>The role of C-X-C chemokine receptor type 4 (CXR-4) in chemotherapy resistance remains crucial in promoting proliferation, invasion, and progression in acute myeloid leukemia (AML) cells. This study aims to screen and investigate a potential lead candidate as a therapeutic agent targeting CXCR-4 in AML cells. Diversity-based virtual screening process using AutoDock-Vina was employed to screen approximately 850,000 compounds from the ChemBridge-small molecule database. The binding stability and dynamics were investigated through GROMACS-based molecular dynamics simulations and root mean square deviation (RMSD). AML cells (THP-1, HL-60, and SKM-1 cell lines) were used to assess proliferation CXCR-4 expression, and apoptosis induction was measured using flow cytometry and trans-endothelial migration was assessed using calorimetric method in AML cells. The absorption, distribution, metabolism, and excretion (ADME) properties were predicted using SwissADME server. The computational evaluations revealed SSB-2548 as a lead candidate that binds stably to CXCR-4. Molecular dynamics simulations provided detailed insights into the conformational changes of the SSB-2548/CXCR-4 complex. The compound inhibited the THP-1, HL-60, and SKM-1 cell proliferations with GI₅₀ values of 84.57, 41.30, and 120.50 nM, respectively. SSB-2548 decreased the trans-endothelial migration and CXCR-4 expression in while inducing early and late phase apoptosis in all three AML cell types. ADME predictions indicated a favorable lead-likeness, gastrointestinal absorption, and lack of notable toxicity. Computational assessments identified SSB-2548 as a novel CXCR-4 inhibitor. In vitro evaluations proved this lead compound effective against AML cells. These findings lay the groundwork for future, investigations positioning SSB-2548 as a candidate for the development of targeted therapies against AML.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143440128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Etomidate Induces Mitochondrial Dysfunction in Glioma Cancer Cells by Inhibiting Mitochondrial Biogenesis Mediated by CREB/PGC-1α.","authors":"Hailiang Shi, Zhongcheng Cao, Kai Wei","doi":"10.1002/bab.2722","DOIUrl":"https://doi.org/10.1002/bab.2722","url":null,"abstract":"<p><p>Gliomas are one of the most prevalent types of solid tumors in the brain. Imbalances in mitochondrial metabolism have been implicated in the pathological progression of gliomas. Etomidate, an agonist of the γ-aminobutyric acid type A (GABAA) receptor, is widely used in clinical settings. In this study, we report a novel pharmacological function of etomidate in regulating mitochondrial metabolism in glioma cancer cells. U87 glioma tumor cells were treated with etomidate (0.5, 1.0, and 2.0 µg/mL) for 24 h. Quantitative real-time PCR, western blot analysis, mtDNA/nDNA ratio, MitoTracker Red staining, Complex I and IV activity, intracellular ATP levels, and mitochondrial respiration were assessed. First, etomidate exposure inhibited the expression of PGC-1α in U87 glioma tumor cells. Further investigation revealed that etomidate suppressed the expression of Nrf1 and TFAM, the two key executors of mitochondrial biogenesis. Etomidate treatment led to damage in mitochondrial biogenesis by decreasing the mtDNA/nDNA ratio, reducing the protein expression of cytochrome B, and lowering mitochondrial mass. These changes suggest impaired mitochondrial replication and function. Correspondingly, etomidate exposure induced a \"loss of mitochondrial function\" by diminishing the activities of Complex I and Complex IV, the mitochondrial respiratory rate (MRR), and ATP generation. These effects highlight the detrimental impact of etomidate on the energy metabolism of glioma cells. Mechanistically, etomidate inactivated the transcription factor CREB by reducing its phosphorylation at Ser133. Activation of CREB with the second messenger cAMP restored the expression of PGC-1α, the mtDNA/nDNA ratio, Complex IV activity, summarized mitochondrial respiratory rate (MRR), and ATP production. This suggests that CREB activation may serve as a potential therapeutic strategy to counteract etomidate's inhibitory effects on mitochondrial function in glioma cells. Our results suggest that damage to mitochondrial biogenesis is a key step in the anticancer properties of etomidate in gliomas, and the decrease in PGC-1α and its downstream molecules may be the critical mechanism behind this effect.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143432570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective Effect and Gut Microbiota Modulation of Grifola frondosa Antioxidant Peptides in Sodium Dextran Sulfate-Induced Ulcerative Colitis Mice.","authors":"Tong Chen, Linhai Hong, Peng Wang, Qian Teng, Fei Fang, Qinghong Liu","doi":"10.1002/bab.2734","DOIUrl":"https://doi.org/10.1002/bab.2734","url":null,"abstract":"<p><p>Grifola frondosa antioxidant peptides (GFAP) were prepared through trypsin enzymolysis and characterized. This study conducted a comprehensive assessment of clinical symptoms, colon pathological injuries, levels of inflammatory factors, expression of inflammation-related proteins, and alterations in gut microbiota composition in mice with ulcerative colitis (UC). The findings demonstrated that GFAP effectively mitigated UC, alleviated mucosal damage, and reduced inflammatory infiltration. Specifically, GFAP administration resulted in significant reductions in pro-inflammatory cytokines IL-6, IL-1β, and TNF-α, while enhancing the expression levels of tight junction proteins such as Occludin and ZO-1. Additionally, GFAP treatment led to decreased levels of Toll-like receptor 4 (TLR-4), inducible nitric oxide synthase (iNOS), and TNF-α. Noteworthy, GFAP also influenced the gut microbiota by decreasing the abundance of Proteobacteria and increasing Bacteroidetes and Firmicutes. Moreover, specific bacteria like Bacteroides uniformis and Alistipes exhibited elevated abundances following GFAP treatment. In summary, GFAP exhibited preventive and protective effects against UC in mice by effectively alleviating clinical symptoms and modulating gut microbiota composition.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143432572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential Anti-Filarial Molecules Against ATP Binding Site of MurE Enzyme: A Molecular Docking and Dynamics Approach to Combat Lymphatic Filariasis.","authors":"Dhamodharan Prabhu, Muthu Krishnan Dhivya Dharshini, Sundararaj Rajamanikandan, Alwar Ramanujam Padmavathi, Palaniyandi Velusamy, Subash C B Gopinath","doi":"10.1002/bab.2727","DOIUrl":"https://doi.org/10.1002/bab.2727","url":null,"abstract":"<p><p>Lymphatic filariasis (LF) is a mosquito-borne disease caused by parasitic nematodes Brugia malayi, Brugia timori, and Wuchereria bancrofti. The drugs available are effective in several cases, and the absence of vaccination is the crucial factor hindering the elimination of LF. The UDP-N-acetylmuramoyl-L-alanyl-D-glutamate-2,6-diaminopimelate ligase (MurE) plays an important role in the peptidoglycan biosynthesis of Wolbachia endosymbiont B. malayi, which are reported to be a vital drug target for bacterial and endosymbiotic hosts. Thus, we selected the ATP binding cavity of MurE as the potential site to screen inhibitors. The MurE structure was modeled using AlphaFold due to the absence of an experimental structure. Structure-based screening identified five potent phytochemicals targeting the ATP binding site with higher Glide scores and affinity. The top five phytochemicals CID 311, CID 445713, CID 441626, CID 39077, and CID 10814 showed a docking score of -16.812, -16.117, -15.668, -15.324, and -13.442 kcal/mol, respectively. Further, the molecular dynamics simulations depicted the binding stability of the phytochemical inhibitors bound to the MurE complex. Moreover, ADME assessment and Density Functional Theory analyses of the predicted compounds have shown acceptable pharmacokinetic properties and high reactivity with the drug target of MurE.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143432571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Preliminary Study of CircRNA Expression Characteristics in the Progression of Colorectal Cancer.","authors":"Shimeng Liu, Yang Ou, Yi Zhu, Jianqi Li, Runze Jiang, Feng Zhao, Xinwei Wang","doi":"10.1002/bab.2732","DOIUrl":"https://doi.org/10.1002/bab.2732","url":null,"abstract":"<p><p>Colorectal cancer (CRC) progression involves complex genetic changes. This study examines circRNA expression in CRC to identify biomarkers for improved diagnosis and staging. The objective of this study is to explore the role of circular RNA (circRNA) in CRC progression and identify specific circRNA biomarkers. Using high-throughput circRNA chip technology, cancerous and adjacent tissues from three CRC patients (staged as T1-3N0M0) were analyzed to identify differentially expressed circRNAs. Bioinformatics analyses, including co-expression network construction, Gene Ontology (GO), and Kyoto Encyclopedia of Genes and Genomes (KEGG) annotations, were performed to evaluate circRNA function and pathways. A total of 404 differentially expressed circRNAs were identified, with significant variations between cancerous and adjacent tissues. Trend analysis revealed that circRNA expression decreased progressively with CRC advancement. Co-expression network analysis highlighted eight key circRNAs, including hsa_circ_0000007, associated with CRC progression. GO and KEGG analyses indicated these circRNAs are involved in ribosome biogenesis, metabolism, and the regulation of G1-S phase transcription through the RB1 gene. The expression of hsa_circ_0000007, hsa_circ_0023608, hsa_circ_0026694, and hsa_circ_0029903 decreased as CRC progressed, suggesting their potential as biomarkers for CRC diagnosis and staging. These findings offer insights into the molecular mechanisms underlying CRC progression.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143432568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}