Biotechnology and applied biochemistry最新文献

{"title":"Immunoinformatics-Based Design of a Multi-Epitope Vaccine Targeting a Conserved Copper-Associated Protein in Neisseria gonorrhoeae.","authors":"Sinethemba H Yakobi, Uchechukwu U Nwodo","doi":"10.1002/bab.70179","DOIUrl":"https://doi.org/10.1002/bab.70179","url":null,"abstract":"<p><p>Neisseria gonorrhoeae poses an urgent public health challenge due to rapidly increasing antimicrobial resistance and the absence of an effective vaccine. Targeting conserved bacterial pathways involved in essential physiological processes may provide new opportunities for vaccine antigen discovery. In this study, we applied an immunoinformatics-based pipeline to explore a conserved copper-associated protein as a potential antigenic source for multi-epitope vaccine design. Analysis of 396 clinical genomes confirmed high assembly quality and identified a highly conserved hypothetical protein (AKOBGLPP_01618) through proteome-wide screening for metal-binding signatures. The candidate exhibited strong predicted antigenicity, high prevalence across isolates (99.5%), and no detectable homology with human proteins. In silico analyses suggested potential copper-binding features based on predicted metal-coordinating residues and conserved genomic context. Epitope mapping identified B-cell epitope-rich regions that were refined into conserved, surface-accessible peptides. Predicted cytotoxic and helper T-cell epitopes were filtered for immunogenicity, safety, and strain conservancy prior to inclusion in a multi-epitope construct containing a TLR4-targeting adjuvant and processing-optimized linkers. The resulting 155-amino-acid construct demonstrated favorable physicochemical characteristics, predicted solubility, and an estimated global population coverage of 74.84%. Docking analyses suggested a consistent interaction propensity with the TLR4/MD-2 complex across multiple predicted conformations. Although these findings provide preliminary computational support for the proposed construct, experimental validation will be required to confirm antigen function, immunogenicity, and receptor engagement. Collectively, this study identifies a conserved copper-associated protein as a potential antigen source and presents a computational framework for exploring vaccine candidates against N. gonorrhoeae.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147833856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytocomponents Derived From Naturally Growing Chamaedorea seifrizii as Novel α-Amylase Inhibitors for Diabetes Management: In Vitro and In Silico Approach.","authors":"Amrita Chauhan, Arun Dev Sharma, Inderjeet Kaur, Anindya Sundar Panja, Sk Aftabul Alam","doi":"10.1002/bab.70178","DOIUrl":"https://doi.org/10.1002/bab.70178","url":null,"abstract":"<p><p>Blocking the enzyme alpha-amylase is a promising approach for treating Type 2 diabetes. Although they have been employed as blood-sugar reducers, medicinal phytocompounds have limited ability to inhibit α-amylase. The bamboo plant Chamaedorea seifrizii is mostly used for its esthetic qualities and air-purifying capabilities. Further study is necessary because there are not many studies on this plant's phytochemical composition and anti-α-amylase capability. Therefore, this study's goal was chemical profiling, followed by an in vitro experiment to assess the anti-α-amylase capability of C. seifrizii's flowers and fruits, and an in silico docking of important bioactive chemicals against α-amylase. The bioactive ingredients in C. seifrizii auxiliary inflorescence acetone extract (CIAE) and C. seifrizii fruit acetone extract (CFAE) were identified using GC-FID. The web program Cb-dock2 was utilized for docking. Additionally, two-dimensional interactions between ligands and the target enzyme were examined. After molecular docking, molecular dynamics (MD) simulations, and molecular mechanics Poisson-Boltzmann surface area (MM-PBSA) computations were performed. The PASS prediction of every ligand and the in silico ADMET pharmacoinformatic perspective were evaluated. The α-amylase inhibition assay was used for wet lab validation. A total of 17 (in CIAE) and 21 (in CFAE) phytocomponents were identified by GC-FID. Major phytocompounds were phenethyl cinnamate (45%) in CIAE and nezukol in (10%) CFAE along with other minor components, such as resveratrol, hinokione, butylated hydroxyanisole, sandaracopimarinol (CFAE), larixol, taxodione, methyl pentanoate, and cedrene epoxide (CIAE). Docking analysis showed that every ligand from CIAE and CFAE effectively bound to the enzyme but with varied affinity. Sandaracopimarinol from CIAE and taxodione from CFAE were detected as best docked compounds with vina score ranging from 8.9 to 8.3, respectively. The stability of docked complexes was further confirmed by the post-MD analysis of the best docked structure, as seen by the root mean square deviation (RMSD) and radius of gyration (R_g) values. MM-PBSA analysis revealed best docked structure with binding energy -72.2 kJ mol^-1. According to the results of the in silico ADMET analysis, all the ligand molecules showed good absorption and did not cause any harmful effects. In a kinetic study, CIAE and CFAE were found to inhibit α-amylase in a noncompetitive way. To best of our knowledge, this is first time study reporting bioactive chemicals from C. seifrizii extracts exhibiting anti-α-amylase potential. To validate these results, more studies utilizing clinical trials and animal models are required. This study claimed that due to richness of phytocompounds, C. seifrizii extracts may help to block the enzyme α-amylase, which leads to the creation of new drugs for diabetes.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147833944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Indigo Dye: From Ancient Extraction to Green Biomanufacturing.","authors":"Simeng Zhang, Xiaoyi Wang, Wei Chen, Jie Zheng, Chengtao Wang, Chenchen Qi, Guohui Chen, Lidan Niu","doi":"10.1002/bab.70161","DOIUrl":"https://doi.org/10.1002/bab.70161","url":null,"abstract":"<p><p>Indigo, a historically significant blue dye widely used in textiles such as denim, was originally derived from plants. However, traditional extraction faced issues like low yield and high land use, while later chemical synthesis relied on toxic compounds, creating environmental and health concerns. Recently, microbial synthesis has emerged as a sustainable alternative, requiring only 10% of the time of plant-based methods and 80% less water than chemical production. Besides textiles, indigo is also applied in medicine, food, cosmetics, and semiconductors. This review covers the history, biosynthesis, detection, and diverse applications of indigo, addressing current challenges and highlighting its potential for eco-friendly dye manufacturing.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147763229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunogenicity, Pharmaceutics, and Properties of Recombinant Aspergillus flavus Uricase Modified by Citric Acid/Ethylenediamine Carbon Dots.","authors":"Hai-Ling Li, Hong-Bin Jia, Jin-Long He, Xiu-Feng Gao, Yong-Sheng Li","doi":"10.1002/bab.70174","DOIUrl":"https://doi.org/10.1002/bab.70174","url":null,"abstract":"<p><p>Clinically, patients with gout are often treated with uricase (Uox)-based drugs. While these medications are effective, many patients suffer from immune rejection associated with their use. To reduce Uox's immunogenicity, it was modified with carbon dots synthesized from citric acid and ethylenediamine (CA/EDA-CDs), forming CA/EDA-Uox. The particle size, zeta potential, and main functional groups of CA/EDA-CDs and CA/EDA-Uox were characterized by transmission electron microscopy (TEM), FT-IR, and dynamic light scattering (DLS). CCK8 and flow cytometry were used to detect the survival rate of L02 cells treated with CA/EDA-CDs and CA/EDA-Uox, respectively. After treatment with 1% CA/EDA-Uox for 2 weeks, IgG and IgM titers in rats were detected by ELISA. The particle size of CA/EDA-CDs is mainly 5 nm, and zeta potential is distributed in -10.3 mV and 5.8 mV. The cell survival rate and the number of fluorescently labeled cells were the highest in 1% CA/EDA-CDs and 1% CA/EDA-Uox treatment groups, respectively. No antibody was detected in 1% CA/EDA-Uox treated rats. The contents of IL-2, 6, 10, and TNF-β in the blood of quails were significantly lower than those in Uox group (p < 0.01). The cytotoxicity of 1% CA/EDA-CDs and 1% CA/EDA-Uox were the lowest; there were less antibodies produced by 1% CA/EDA-Uox in rats. CA/EDA-Uox exhibits satisfactory safety, low immunogenicity, and favorable pharmacological properties. It holds promise for progression to the clinical trials and eventual clinical application.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147763156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation and Characterization of PVA-SA Hydrogel Immobilized β-Galactosidase With Improved Catalytic Properties.","authors":"Doruk Akdoğan, Ayşegül Peksel","doi":"10.1002/bab.70177","DOIUrl":"https://doi.org/10.1002/bab.70177","url":null,"abstract":"<p><p>This study reports the immobilization of Aspergillus oryzae β-galactosidase in a polyvinyl alcohol-sodium alginate (PVA-SA) hydrogel and its performance compared with the free enzyme. Through immobilization, the optimum temperature shifted from 40°C to 60°C, with the immobilized enzyme retaining high activity and exhibiting improved resistance to heat-induced inactivation. Although maximum catalytic activity was observed at pH 5.0 for both free and immobilized forms, the immobilized enzyme sustained higher stability in near-neutral and slightly alkaline environments. Kinetic analysis revealed that the K_m value increased from 0.21 to 0.33 mM, indicating diffusion limitations, whereas the apparent V_max rose from 0.40 to 1.71 U/mg protein. Storage experiments demonstrated improved stability at 4°C, with about 60% of the initial activity retained after 8 weeks, whereas freezing at -20°C accelerated inactivation. The immobilized enzyme retained more than 80% of its catalytic performance after three consecutive uses and still preserved approximately 65% following the fourth cycle. Lactose hydrolysis experiments confirmed efficient and sustained performance, reaching 78% conversion after 180 min.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147763159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural Characterization and Anti-Inflammatory Activities of Polysaccharide From Hericium erinaceus Mycelium.","authors":"Chong Sun, Liuya Wang, Yushan Shi, Xueqing Geng, Huiqing Sun, Tengda Li, Qi Zhu, Lanfeng Hui, Xiaojian Wu, Lijing Chen, Zhenyuan Zhu","doi":"10.1002/bab.70148","DOIUrl":"https://doi.org/10.1002/bab.70148","url":null,"abstract":"<p><p>A novel heteropolysaccharide named HEP-S was separated from the mycelium of Hericium erinaceus using hot water extraction. The structure of HEP-S (2.45 × 10⁶ Da) is mainly composed of →4,6)-α-D-Glcp(1→, →2)-α-D-Manp(1→, →4)-α-D-Glcp(1→, →3,6)-β-D-Glcp(1→, T-β-D-Galp(1→, and →4)-β-D-Galp(1→. In addition, we investigated the anti-inflammatory activity and mechanism of HEP-S in lipopolysaccharide-stimulated RAW264.7 cells. HEP-S (20, 40, and 80 µg/mL) could suppress the production of inflammatory cytokines by reducing vital transcription factors in the nuclear factor-κB signaling (NF-κB) pathway. The results showed that HEP-S exhibited notable anti-inflammatory activity on RAW 264.7, demonstrating its potential therapeutic effect on relieving gastritis.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147763232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the Power of Strigolactones in Abiotic Stress Management: A Comprehensive Review.","authors":"Amandeep Singh, Nahida Rehman Mir, Roohi Sharma, Arun Dev Singh, Manu Sharma, Puja Ohri, Renu Bhardwaj, Nitika Kapoor","doi":"10.1002/bab.70175","DOIUrl":"https://doi.org/10.1002/bab.70175","url":null,"abstract":"<p><p>In the face of continuous climate change, environmental stress has become a major threat to agricultural productivity. To address these challenges, one key strategy is the application of phytohormones like abscisic acid, ethylene, auxins, gibberellins, cytokinins, salicylic acid, jasmonates, brassinosteroids, and strigolactones (SLs). These hormones are important for supporting overall plant growth under stress, as they activate the key signaling pathways that help plants adapt to adverse conditions and mitigate productivity losses. Among these crucial phytohormones, SLs have gained attention for their unique act in plant adaptation to stress. Strigolactones are a newly identified class of phytohormones synthesized from carotenoids and were first identified as ecological signals involved in triggering the germination of parasitic seeds and facilitating symbiotic interactions between plants and beneficial microbes. Further studies have revealed their involvement in diverse developmental processes, such as root growth, shoot branching, reproductive development, and leaf senescence. Hence, the present review focuses on the roles of SLs in plant development and stress responses, covering their discovery, biosynthesis, and signaling pathways. It emphasizes the significance of SLs in mitigating abiotic stresses, including heavy metal toxicity, thermal stress, nutrient deficiencies, and oxidative stress. Finally, it outlines the future research directions and the potential of SLs to enhance plant resilience and productivity in changing environments.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147763225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating In Vitro Culture, Phytochemical Analysis, and DNA Markers for Comprehensive Characterization of Ocimum sanctum and Ocimum basilicum.","authors":"Priya Singh, Hemant Deorukar, Priti Deshmukh, Jyoti Deshpande, Minal Wani","doi":"10.1002/bab.70171","DOIUrl":"https://doi.org/10.1002/bab.70171","url":null,"abstract":"<p><p>The recognized medicinal potential of Ocimum sanctum (holy basil) and Ocimum basilicum (sweet basil) is largely underpinned by the structural diversity and abundance of their phytochemical constituents. The two species are well documented with regard to their medicinal effects, but there is a lack of comparative investigations linking in vitro biosynthetic potential with genetic diversity. Callus cultures allow for a sustainable alternative that can produce bioactive compounds similar to intact plants without the pressure exerted on natural populations. The present study compared the callus induction response, phytochemical profiles, and RAPD-based molecular diversity of O. sanctum and O. basilicum. Maximum callus proliferation was observed in O. sanctum on MS supplemented with 1.0 mg L^-1 2,4-D + 0.2 mg L^-1 BAP, while 0.2 mg L^-1 NAA + 0.2 mg L^-1 BAP was optimal for O. basilicum. Furthermore, in terms of analysis of the total yield, higher total phenolic and flavonoid contents have been detected in callus extracts and leaves of O. sanctum than in O. basilicum. HPLC analysis confirmed caffeic acid, which is the main compound in both species, rosmarinic acid, and kaempferol, the latter being exclusively found in O. sanctum. RAPD showed various polymorphic banding patterns indicative of polymorphism between the two species. The work correlated the in vitro phytochemical biosynthesis with the molecular level variations in genetics, which can be beneficially grasped for future metabolic exploitation, cultivar selection, and conservation strategies.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147721875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Network Pharmacology and Bioinformatics Approach for Determining the Mechanisms and Molecular Targets of Catechins Against Epilepsy.","authors":"Punam Salaria, Desu Gayathri Niharika, Satyanarayana K Konavarapu, Amarendar Reddy M, Sung Yeol Kim","doi":"10.1002/bab.70176","DOIUrl":"https://doi.org/10.1002/bab.70176","url":null,"abstract":"<p><p>Epilepsy is a debilitating neurological disorder that impacts approximately 50 million people worldwide. The treatment of epilepsy with antiepileptic drugs has not achieved effective seizure management and thus requires new therapeutic options. This study investigated the catechins' affect on epilepsy-related molecular targets using a computational method that combined network pharmacology, molecular docking, and molecular dynamics (MDs) simulation. We fetched 84 catechins-related and 5356 disease-associated targets from various databases, yielding 31 common targets. The protein-protein interaction (PPI) network of 31 common targets identified 10 hub genes, including ALB, INS, brain-derived neurotrophic factor (BDNF), PTGS2, tumor necrosis factor (TNF), IL1B, FOS, IL6, LEP, and FGF2. Further, the functional enrichment analysis revealed that these common targets have a high prevalence in multiple pathways and gene ontology functions. Furthermore, \"compound-target\" and \"compound-gene-pathway\" networks were constructed and analyzed. Network pharmacology data show TNF, IL1B, and IL6 could influence epilepsy treatment by regulating several pathways. The Cresset Flare Pro+ docking study unveiled that the lead catechin, epigallocatechin gallate (EGCG), exhibited the highest Lead Finder (LF) dG scores of -10.2, -9.40, and -8.15 kcal/mol against TNF, IL6, and IL1B, respectively. The electrostatic complementarity and Molecular Mechanics with Generalized Born and surface area (MMGBSA) results supported the docking results. Further, the stability of EGCG-bound complexes was analyzed using a 300 ns MD simulation. The principal component analysis yielded promising results for the EGCG-2AZ5 and EGCG-1ALU complexes collective motion. These findings provide computational evidence suggesting that EGCG has a promising scaffold for designing multi-target molecules that could modulate epilepsy, meriting further experimental validation.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147721956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High Expression of PDIA4 Contributes to Poor Prognosis in Glioma","authors":"Yang Liu,&nbsp;Hua He,&nbsp;Yourui Zou,&nbsp;Jin Feng,&nbsp;Haibo Liu,&nbsp;Peng Gao,&nbsp;Wenchao Lu,&nbsp;Yang Zhao,&nbsp;Hui Ma","doi":"10.1002/bab.70042","DOIUrl":"10.1002/bab.70042","url":null,"abstract":"<div>\u0000 \u0000 <p>This study aimed to investigate the expression and prognostic value of protein disulfide isomerase A4 (PDIA4) in glioma patients. We initially analyzed the expression of PDIA4 in various tumors using the Gene Expression Profiling Interactive Analysis 2 (GEPIA2) database. Then, we performed a retrospective analysis of clinical and pathological data of 118 patients with glioma from January 2015 to January 2019 at the Department of Neurosurgery, General Hospital of Ningxia Medical University. Additionally, normal brain tissue samples were obtained from three patients undergoing surgery for intracerebral hemorrhage. Immunofluorescence was utilized to evaluate PDIA4 expression in gliomas and normal brain tissues, and we analyzed the correlation between PDIA4 expression, tumor malignancy grade, and survival prognosis. GEPIA2 dataset analysis showed significantly higher PDIA4 expression in most tumor tissues compared to normal tissues, particularly in gliomas. Immunofluorescence revealed PDIA4 localization, primarily in the cytoplasm of tumor cells. Amongst 118 patients, PDIA4 expression positively correlated with age (<i>p</i> &lt; 0.005), WHO tumor grade (<i>p</i> &lt; 0.0001), but showed no correlation with gender, tumor size, or tumor location. Notably, glioma patients with high PDIA4 expression demonstrated significantly lower overall survival rates than those with low expression (<i>p</i> &lt; 0.0001), especially in low-grade glioma patients (<i>p</i> &lt; 0.0001), while no significant correlation was found in high-grade glioma patients (<i>p</i> &lt; 0.1356). Multivariate analysis revealed that elevated high PDIA4 expression is an independent prognostic factor associated with adverse outcomes in glioma patients (<i>p</i> &lt; 0.0001). PDIA4 emerges as a novel molecular biomarker for prognostic prediction in glioma patients. Its elevated expression is associated with unfavorable outcomes, highlighting its potential as both a therapeutic target and a prognostic indicator in glioma management.</p>\u0000 </div>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":"73 2","pages":"528-536"},"PeriodicalIF":2.7,"publicationDate":"2026-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144943736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}