Biotechnology and applied biochemistry最新文献

{"title":"Aquaporin 9: Exacerbation of Vulnerable Carotid Plaque Formation.","authors":"Bin Xu, Yifei Zheng, Yi Wang, Jie Sun, Dehai Lang, Qiyang Xu","doi":"10.1002/bab.2776","DOIUrl":"https://doi.org/10.1002/bab.2776","url":null,"abstract":"<p><p>Aquaporin 9 (AQP9) expression is significantly elevated in vulnerable carotid plaque (VCP). Hence, we probed the mechanism of AQP9 in VCP formation. The VCP model was established in ApoE^-/- C57BL/6 mice. Dataset GSE163154 was analyzed by R software. Human aortic endothelial cells (HAECs) were incubated with 50 µg/mL oxidized low-density lipoprotein (ox-LDL) and 20 mM l-(+)-lactic acid for 24 h. Mice (AQP9 overexpression plasmid) and HAECs (AQP9 overexpression/dynamin-related protein 1 [DRP1] silencing plasmids) were infected by lentivirus. Mouse plasma lipid level was estimated. The histopathological condition of model mice was observed by oil red lipid staining, hematoxylin-eosin (H&E) staining, and Masson staining. Levels of AQP9 and DRP1 in model mice and HAECs were quantified by quantitative real-time polymerase chain reaction (qRT-PCR). Levels of AQP9, DRP1, and mitochondrial fission-/endothelium-mesenchymal transition (EndMT)-related factors in model mice and HAECs were assayed by western blot. Lactate level in model mice was detected. Promoter histone lactylation level of DRP1 was measured by chromatin immunoprecipitation (ChIP). Behaviors of HAECs were tested by cell counting kit-8 (CCK-8), colony formation test, and scratch test. AQP9 was highly expressed in intraplaque hemorrhage patients. AQP9 overexpression promoted levels of DRP1, lactate, histone lactylation, mitochondrial fission factor, vimentin, and N-cadherin, while inhibiting vascular endothelial (VE)-cadherin level and plaque stability in model mice and facilitating viability, proliferation, and migration of HAECs. DRP1 silencing reversed the impacts of AQP9 overexpression on cell viability, proliferation, migration, and levels of mitochondrial fission-/EndMT-related factors in HAECs. AQP9 enhances DRP1-mediated mitochondrial fission by lactate and thus promotes EndMT to exacerbate the VCP formation.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143980365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mathematical Correlation of Yield Coefficients Based on Individual Reaction of Cell and Product Formation and Total Metabolic Reaction in Biological Reaction Kinetic Modeling.","authors":"Swatika Juhana, Fitri Nur Kayati, Aris Sudomo, Arini Wresta","doi":"10.1002/bab.2773","DOIUrl":"https://doi.org/10.1002/bab.2773","url":null,"abstract":"<p><p>Kinetic models are important tools required in designing biological reactors and can be developed through mass balance equations arrangement based on kinetic data of microorganism metabolic reactions. Yield coefficients are an important parameter in the arrangement of mass balance equations as they can quantitatively link substrate consumption to cell and product formation. We know two forms of yield coefficients of cell and product formation that have different uses. The first coefficients are derived from the stoichiometric equation of overall metabolic reaction and the second are derived from the parallel reactions of cell formation, product formation, and maintenance. Since these types of yield coefficients are usually written in the similar pattern, these coefficients are frequently misused. A previous researcher has defined and differentiated the use of these two types of yield coefficients to avoid inappropriate uses that can cause an error in the calculation results. To complete and clarify the definition, this communication discusses the mathematical correlation between these two types of yield coefficients using the latest thermodynamic approach for stoichiometric coefficients estimation of metabolic reaction.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143980366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RETRACTION: Design and Characterization of a Chimeric Multiepitope Construct Containing CfaB, Heat-Stable Toxoid, CssA, CssB, and Heat-Labile Toxin Subunit B of Enterotoxigenic Escherichia Coli: A Bioinformatic Approach.","authors":"","doi":"10.1002/bab.2774","DOIUrl":"https://doi.org/10.1002/bab.2774","url":null,"abstract":"","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":"e2774"},"PeriodicalIF":3.2,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143989490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revealing the Taxonomic Profile of a Wild Edible Mushroom From Tribal Cuisine and Its Pharmacological Significance.","authors":"Gouri Basak, Niranjan Roy, Arun Kumar Dutta, Krishnendu Acharya","doi":"10.1002/bab.2779","DOIUrl":"https://doi.org/10.1002/bab.2779","url":null,"abstract":"<p><p>The historical use of wild mushrooms as food and medicine has been documented in several countries, including India. The Indian macrofungal flora consists of a diverse range of distinct species that have long been valued by various tribal groups for their medicinal and culinary qualities. During the process of documenting indigenous mushroom species, one unique edible Russula sp. was collected from the Pinus-dominated forest of the East Khasi Hills district of Meghalaya. Following a thorough assessment of the morphological traits and molecular phylogenetic analysis, this distinct species has been identified as Russula pseudojaponica, a new record from India. In order to investigate its therapeutic potential, a methanolic fraction was prepared and characterized for myco-chemicals. A substantial quantity of different secondary metabolites like carotenoids, ascorbic acid, and phenolics has been detected. The methanolic fraction also had notable antioxidant activity, displaying strong capabilities in scavenging DPPH and ABTS radicals, possessing high reducing power, and chelating capacity. In addition, the extract demonstrated the capacity to hinder the growth of Listeria monocytogenes, Bacillus subtilis, Pseudomonas aeruginosa, Escherichia coli, Salmonella typhi, and Staphylococcus aureus, as seen in the broth microdilution experiment. Furthermore, it induced morphological changes in bacterial cells, which were seen by scanning electron microscopy. Moreover, the extract demonstrated the ability to inhibit the proliferation, clonogenicity, and migratory potential of lung carcinoma cells, suggesting its potential as an anticancer agent. The extract successfully arrested the cell cycle progression in the S phase of cell division. Involvement of the intrinsic mitochondrial pathway of apoptosis was also observed after extract treatment. Hence, based on the findings of this study, this exceptional fungus has the capacity to function as an organic reservoir for the creation of novel medications to counteract illnesses associated with oxidative stress, bacterial ailments, and cancer.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143954760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Novel Therapeutic Strategy for Ameliorating Hyperglycemia-Induced Liver Injury via Overexpression of the Carboxyl Terminus of HSP70-Interacting Protein in Wharton's Jelly Mesenchymal Stem Cells.","authors":"Shun-An Kan, Ayaz Ali, Shih-Wen Kao, Bruce Chi-Kang Tsai, Yueh-Min Lin, Dennis Jine-Yuan Hsieh, Chia-Hua Kuo, Wei-Wen Kuo, Shinn-Zong Lin, Chih-Yang Huang","doi":"10.1002/bab.2771","DOIUrl":"https://doi.org/10.1002/bab.2771","url":null,"abstract":"<p><p>Diabetes is a widespread metabolic disorder that significantly affects modern society. The liver plays a vital role in metabolism; however, hyperglycemia can induce liver damage and disrupt its normal functions. Wharton's jelly mesenchymal stem cells (WJMSCs) engineered to express the carboxyl terminus of HSP70-interacting protein (CHIP) have demonstrated protective effects against hyperglycemia-induced damage in various organs. Nonetheless, the potential hepatoprotective effects and underlying mechanisms of these modified stem cells in diabetic livers remain unclear. Therefore, this study aimed to evaluate the efficacy of CHIP-transfected WJMSCs in mitigating hyperglycemia-induced hepatic injury in diabetic rats and to elucidate the associated protective mechanisms. Diabetic rats received tail vein injections of WJMSCs either overexpressing or silenced for CHIP. Seven weeks post-transplantation, all rats were sacrificed, and liver tissues were harvested for histological staining and Western blot analysis. The findings indicated that CHIP-overexpressing WJMSCs significantly reversed hyperglycemia-induced liver damage, reducing tissue injury, fibrosis, and glycogen deposition. These cells also alleviated hepatic inflammation and apoptosis. Moreover, they regulated oxidative stress pathways by lowering gp91-phox, Rac1, and phosphorylated PKCζ levels, while enhancing phosphorylated Nrf2 and SOD-2 expression. Additionally, the modified WJMSCs suppressed STAT3 activation and downregulated FOXO3a, suggesting a role in attenuating fibrosis and triglyceride accumulation in diabetic livers. Overall, CHIP-overexpressing WJMSCs reversed hyperglycemia-induced hepatic alterations by mitigating inflammation and oxidative stress while also modulating pathways related to fibrosis and lipid metabolism. These results highlight the therapeutic potential of CHIP-modified WJMSCs in managing diabetic liver complications and offer promising avenues for future treatment strategies.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":"e2771"},"PeriodicalIF":3.2,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143966239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering of Ruthenium-Based Anticancer Drug RAPTA-C and Carvacrol Incorporated Hyaluronic Acid-Altered Liposome Formulation for Ovarian Cells and Its Apoptosis Induction.","authors":"Xin Huang","doi":"10.1002/bab.2770","DOIUrl":"https://doi.org/10.1002/bab.2770","url":null,"abstract":"<p><p>Ovarian cancer (OC) is the fifth most common cancer in women, resulting in more deaths than any other female reproductive system disease. This investigation presents hyaluronic acid (HA)-altered liposome encapsulating 1,3,5-triaza-7-phosphaadamantane compound (RAP) and carvacrol (CAR) (RAP-HA@Lipo/CAR) for targeted delivery to enhance antitumor and antimetastatic efficacy in OC. The co-delivered liposomes enhanced anticancer and synergic activity in A2780 OC cells. Further, the apoptotic potential of RAP-HA@Lipo/CAR was assessed by acridine orange (AO)/propidium iodide (PI) and 4,6-diamidino-2-phenylindole dihydrochloride (DAPI)/PI staining techniques. The antimetastatic potential of RAP-HA@Lipo/CAR was evaluated using wound healing and migration assays. Our findings indicated that HA alteration enhanced the cell uptake of the developed RAP-HA@Lipo/CAR in A2780 cell lines. This study demonstrates a tumor-targeting RAP-HA@Lipo/CAR delivery promising to treat OC cells.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":"e2770"},"PeriodicalIF":3.2,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143967657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel Sulfonylhydrazones With Sulfonate Ester Framework: Promising Dual Inhibitors of AChE and hCAs.","authors":"Cansu Öztürk, Neslihan Balci, Osman Nuri Aslan, Erbay Kalay","doi":"10.1002/bab.2780","DOIUrl":"https://doi.org/10.1002/bab.2780","url":null,"abstract":"<p><p>Alzheimer's disease (AD) is a progressive neurodegenerative disorder that affects millions of individuals worldwide. Treatment strategies for AD vary depending on cognitive and behavioral symptoms, with cholinergic replacement therapy using acetylcholinesterase (AChE) inhibitors being one of the primary approaches. Recent studies have also identified human carbonic anhydrases (hCAs) as significant therapeutic targets for AD, offering new opportunities for the development of innovative treatments. Carbonic anhydrase inhibitors have been shown to prevent early mitochondrial damage and inhibit H₂O₂ production, thereby reducing amyloid plaque formation. Building on the promising potential of hydrazones particularly sulfonyl hydrazones as anticholinesterase agents, we synthesized 12 novel chlorine-substituted sulfonyl hydrazone compounds containing aryl sulfonate ester groups. These compounds were evaluated for their inhibitory effects on AChE, hCA I, and hCA II enzymes. The synthesized compounds exhibited low nanomolar inhibitory activity, with K_i values ranging from 9.58 ± 2.22 to 104.04 ± 23.82 nM for AChE, 9.12 ± 2.21 to 477.63 ± 218.52 nM for hCA I, and 17.54 ± 7.74 to 564.62 ± 213.98 nM for hCA II. Notably, compound 6 showed strong inhibitory activity against hCA I (K_i = 9.12 ± 2.21 nM; acetazolamide (AZA) = 26.54 ± 3.11 nM) and hCA II (K_i = 17.54 ± 7.74 nM; AZA = 21.73 ± 2.42 nM), whereas compound 4 exhibited superior AChE inhibition (K_i = 9.58 ± 2.22 nM; TAC = 23.12 ± 2.05 nM). The chemical structures of the synthesized compounds were characterized using advanced spectroscopic techniques, including FT-IR, ¹H-NMR, and ¹³C-NMR spectroscopy.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":"e2780"},"PeriodicalIF":3.2,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143982558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum lncRNA PVT1 and Its Targets miR-146a and SIRT1 as Biomarkers for the Early Detection of Breast Cancer and Its Metastasis.","authors":"Noha H Sayed, Mahmoud A Senousy, Olfat G Shaker, Mona A Kortam","doi":"10.1002/bab.2769","DOIUrl":"https://doi.org/10.1002/bab.2769","url":null,"abstract":"<p><p>Early detection of breast cancer (BC) and its metastasis greatly improves the patients' outcomes. The long noncoding RNA plasmacytoma variant translocation 1 (PVT1) and its targets, microRNA-146a and sirtuin 1 (SIRT1), affect BC development; however, their predictive abilities in early detection of BC and its metastatic potential are largely unknown. This study investigated serum PVT1, miR-146a, and SIRT1 as potential diagnostic and predictive biomarkers for BC and its metastasis. 120 BC patients, 40 breast fibroadenoma (BFA) patients, and 80 healthy volunteers were enrolled. An upregulation of serum PVT1 expression and SIRT1 protein levels was observed in BC patients compared to controls and BFA patients. miR-146a was downregulated in BC and BFA patients compared to controls, with lower levels in BFA compared to BC. Metastatic BC patients showcased higher PVT1 and SIRT1 and lower miR-146a levels than the nonmetastatic group. PVT1 and miR-146a were associated with the risk of developing BC among healthy controls. Combining PVT1 and miR-146a in a predictive panel showed substantial diagnostic accuracy for BC (area under the curve [AUC] = 0.91, 95% CI = 0.87-0.95). Only PVT1 was a predictor of the risk of developing BC among BFA cases and the risk of metastasis among BC patients. In BC, PVT1 was negatively correlated with miR-146a and positively correlated with SIRT1 and invasive lobular tumor type. Conclusively, our results highlight the PVT1/miR-146a/SIRT1 trajectory as a potential biomarker for early diagnosis and prognosis of BC. The study introduces the PVT1/miR-146a panel as an excellent biomarker for early BC diagnosis and uncloaks the predictive power of PVT1 in terms of breast tumor malignancy and metastatic tendency.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":"e2769"},"PeriodicalIF":3.2,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143977047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of Bentonite and Charcoal for the Purification of Pectinases From Aspergillus niger ATCC 9642.","authors":"Ana Paula Rossetto, Márcia Santin Trentin, Rosicler Colet, Valéria Borszcz, Ana Luiza Lira, Sabrina Duarte Camargo, Geciane Toniazzo Backes, Jamile Zeni, Eunice Valduga","doi":"10.1002/bab.2778","DOIUrl":"https://doi.org/10.1002/bab.2778","url":null,"abstract":"<p><p>The objective of the study was to concentrate and increase the purity of pectinases (exo-polygalacturonase-exo-PG, pectin methylesterase-PME, pectin lyase-PL) obtained from solid-state fermentation by Aspergillus niger ATCC 9642, using activated charcoal and active sodium bentonite. The chemical adsorbents were used at concentrations of 1, 5, 10, 20, 40, and 60 g/L to evaluate the efficiency of purification parameters (purification factor-PF and enzyme recovery-R) of pectinases (exo-PG, PME, and PL). When employing a concentration of 60 g/L of activated charcoal, a PF of 14.15 and 1.91 times and recoveries of 100% and 57% were obtained for exo-PG and PME, respectively. For PL, using a concentration of 40 g/L of activated charcoal, the maximum PF was 2.01 times and the recovery was 138%. With the use of active sodium bentonite (60 g/L), it was possible to obtain a PF of 4.20 times and a recovery of 139% for exo-PG, 2.2 times and 70% for PME, and 0.57 times and 48% for PL, respectively. Activated charcoal, especially at higher concentrations, showed the best results for exo-PG and PME, whereas active sodium bentonite excelled in the purification of exo-PG. The results reinforce the feasibility and efficiency of using chemical adsorbents in the precipitation, concentration, and recovery of pectinases, contributing to the production of enzyme preparations with higher purity and yield for industrial applications.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143954674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lipase: Recombinant Production Methods, Origins, and Industrial Uses.","authors":"Sajad Ehtiati, Seyyed Hossein Khatami","doi":"10.1002/bab.2781","DOIUrl":"https://doi.org/10.1002/bab.2781","url":null,"abstract":"<p><p>Lipases are indispensable enzymes with a wide array of industrial applications. They excel at breaking down triglycerides and are essential in food processing, cleaning agents, biofuel production, environmental remediation, and pharmaceuticals. Microbial sources, particularly bacteria and fungi, dominate lipase production due to their high efficiency, cost-effectiveness, and ability to target specific molecular structures. The advent of genetic engineering has further revolutionized lipase production by enabling the development of tailored enzymes that meet precise industrial needs. As eco-friendly biological catalysts, lipases are pivotal in advancing sustainable and resource-efficient practices, offering significant advantages over traditional chemical methods. Their role in innovation spans increased efficiency, reduced environmental impact, and enhanced specificity across multiple sectors. These qualities establish lipases as vital tools in modern biotechnology, reinforcing their ongoing significance in fostering industrial progress and environmental stewardship.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143976959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}