Biotechnology and applied biochemistry最新文献

{"title":"The Impact of Hunched Posture on the Elasticity of the Upper and Middle Trapezius Muscles During Sedentary Activity.","authors":"Rama Krishna Reddy Guduru, S S Saravana Kumar, M M Ramakrishna, Joshna Ramineni, Bennila Thangammal Chandrasekaran, Shiva Kumar Badiganchela, S R Mugunthan","doi":"10.1002/bab.70008","DOIUrl":"https://doi.org/10.1002/bab.70008","url":null,"abstract":"<p><p>Prolonged sitting is often associated with poor posture, hunched shoulder posture (HSP), which may alter the mechanical properties of key muscles such as the upper trapezius (UT) and middle trapezius (MT). This study aimed to investigate the effect of HSP on the elasticity of the UT and MT muscles during a sitting task and to gain insight into the musculoskeletal risks associated with postural errors while sitting. Thirty-four asymptomatic individuals participated in this study between the ages of 25 and 40, who led sedentary lifestyles with 7 h of sedentary activity per day. None of the participants had a history of neurological, systemic, or musculoskeletal diseases that may affect muscle function. The MyotonPRO device was used to measure UT and MT muscle elasticity during normal shoulder posture (NSP) and HSP. Statistical analyses were performed to compare the mechanical properties of the UT and MT muscles across the different postural groups. The findings revealed that the mean elasticity of the UT increased from 1.25 (±0.30) under NSP to 1.30 (±0.32) under HSP. The MT exhibited a more pronounced change, with mean elasticity rising from 1.73 (±0.30) under NSP to 2.12 (±0.34) under HSP. These results indicate significant adaptations in muscle elasticity due to poor postural alignment, suggesting a compensatory response to the mechanical demands of HSP (p < 0.001). The study highlights the detrimental effects of hunched posture on the mechanical properties of the upper and MT muscles, emphasizing the need for ergonomic interventions to promote better postural alignment among sedentary workers. Regular breaks and exercises aimed at improving posture are recommended to mitigate the risks associated with prolonged poor posture, ultimately enhancing worker well-being and productivity.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144309552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association Between Different Types of Physical Activity and the Risk of COPD: A Cross-Sectional NHANES Study.","authors":"Lindong Yuan, Juan Zheng, Zhe Chen","doi":"10.1002/bab.70004","DOIUrl":"https://doi.org/10.1002/bab.70004","url":null,"abstract":"<p><p>Chronic obstructive pulmonary disease (COPD) is a progressive condition that significantly impairs respiratory function. While known risk factors such as smoking and air pollution are well established, the role of physical activity in the development of COPD remains unclear. This study aimed to explore the association between different types of physical activity and COPD risk using data from 14,579 adults aged 40 years and older who participated in the National Health and Nutrition Examination Survey (NHANES) from 2007 to 2018, representing a weighted population of approximately 93.9 million. A weighted binary logistic regression model was employed to evaluate the relationship between various forms of physical activity-leisure, occupational, and transportation-related-and COPD risk, adjusting for multiple covariates including age, sex, race, BMI, smoking status, sedentary time, and comorbidities. COPD was defined based on self-reported physician diagnosis and spirometry criteria. Among the participants, 8.6% had COPD. Results showed that individuals who engaged in moderate-intensity leisure physical activity had a significantly lower risk of COPD (OR: 0.75; 95% CI: 0.61.0.91; P = 0.005). No significant associations were found for high-intensity leisure activity, work-related physical activity, or walking/cycling as a mode of transportation. Subgroup analyses indicated that the protective effect of moderate-intensity leisure activity varied across demographic and behavioral subgroups. These findings suggest that only moderate-intensity leisure physical activity is associated with reduced COPD risk, highlighting the potential preventive role of this specific type of exercise.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144309550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and Functional Characterization of a Lipopeptide Biosurfactant From a Novel Novosphingobium lentum TR17 Strain With Potential for Industrial and Bioremediation Applications.","authors":"Atipan Saimmai, Suppasil Maneerat, Wiboon Riansa-Ngawong, Kattinat Sagulsawasdipan, Chanika Saenge Chooklin","doi":"10.1002/bab.2788","DOIUrl":"https://doi.org/10.1002/bab.2788","url":null,"abstract":"<p><p>Biosurfactant-producing bacteria were isolated from used lubricating oil (ULO)-contaminated soil in Phuket and Trang, southern Thailand. Screening for biosurfactant production was conducted using surface tension reduction and emulsification activity. The highest surface tension reduction was observed in a strain identified as Novosphingobium lentum TR17 using 16S rRNA gene analysis. The selected strain demonstrated optimal biosurfactant production using glucose and commercial monosodium glutamate as carbon and nitrogen sources, respectively. After 60 h of cultivation, the biosurfactant yield reached 5.30 g/L, with a surface tension reduction from 72 to 25 mN/m. The biosurfactant, recovered using a chloroform:methanol solvent system, exhibited thermal and pH stability, a low critical micelle concentration (9 mg/L), and high emulsification activity. Fourier-transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR), and mass spectrometry confirmed its lipopeptide nature. Additionally, the crude biosurfactant demonstrated antibacterial properties and enhanced the solubility of polyaromatic hydrocarbons (PAHs), suggesting its potential for bioremediation and industrial applications.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":"e2788"},"PeriodicalIF":3.2,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144309551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis, Characterization, Biological Properties, and Sensor Applications of Novel Thiosemicarbazone Derivatives.","authors":"Hayreddin Gezegen, Oguz Özbek, Meryem Topal, Hatice Şahin, Ümit M Koçyiğit","doi":"10.1002/bab.70001","DOIUrl":"https://doi.org/10.1002/bab.70001","url":null,"abstract":"<p><p>In this study, novel thiosemicarbazone derivative molecules (5a-j) were synthesized. The characterization of the synthesized molecules was carried out using ¹H-, ¹³C-NMR, FT-IR, and Q-TOF spectroscopic techniques. Then, the inhibitory effect of these compounds was examined for human carbonic anhydrase I and II (hCA I, and II) and acetylcholinesterase (AChE). The hCA I II, and AChE were efficiently inhibited by synthesized thiosemicarbazone derivatives, with Ki values in the range of 0.293-1.666 µM for hCA I, 0.298-1.332 µM for hCA II, and 52.51-138.63 µM for AChE, respectively. Finally, the sensor properties of the synthesized thiosemicarbazone derivatives were investigated. The newly synthesized thiosemicarbazone derivative molecules exhibited high selectivity towards Pb²⁺ ions. The sensor prepared with 5a from these molecules had a Nernstian behavior of 28.8 ± 1.5 mV/decade and a low detection limit of 2.86 × 10^-6 M in a wide concentration range of 1.0 × 10^-1-1.0 × 10^-5 M. The newly proposed sensor also had a wide pH working range (5.0-10.0), a very fast response time (5 s), and good reproducibility. The newly fabricated sensors were used as indicator electrodes for the potentiometric titration of Pb²⁺ ions with ethylenediaminetetraacetic acid (EDTA) and were successfully applied for the determination of Pb²⁺ ion content in different water samples.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144246571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Fluorescent Aptamer Sensor for Detecting Golgi Protein 73 Based on Magnetic Ferroferric Oxide@Boron, Nitrogen-Doped Graphene Quantum Dots.","authors":"Guiyin Li, Wei He, Ruijie Yan, Xuanlong Su, Tingting Yu, Xiaohong Tan, Yong Huang","doi":"10.1002/bab.70006","DOIUrl":"https://doi.org/10.1002/bab.70006","url":null,"abstract":"<p><p>In this paper, a fluorescent aptamer sensor based on magnetic ferroferric oxide@boron, nitrogen-doped graphene quantum dots (Fe₃O₄@B,N-GQDs) was developed to detect Golgi protein 73 (GP73). GP73 aptamer (GP73_Apt) functioned with Fe₃O₄@B,N-GQDs (Fe₃O₄@B,N-GQDs-GP73_Apt) and was used as the fluorescent donor, while molybdenum disulfide (MoS₂) with a large surface served as the fluorescent acceptor. The fluorescence of Fe₃O₄@B,N-GQDs-GP73_Apt was quenched by MoS₂ based on the fluorescence resonance energy transfer (FRET) principle. However, in the presence of GP73, Fe₃O₄@B,N-GQDs-GP73_Apt could specifically bind to GP73, forming a Fe₃O₄@B,N-GQDs-GP73_Apt-GP73 complex. This binding event caused Fe₃O₄@B,N-GQDs-GP73_Apt to move away from the surface of MoS₂, thus blocking the FRET process and recovering the fluorescence. Under optimal conditions, a linear relationship was established between the fluorescence recovery and the concentration of GP73 within the range of 10-1000 ng/mL (R²= 0.9918), and the limit of detection was 7.37 ng/mL. Additionally, when the sensor was applied to test actual samples, the recovery rates were in the range of 98.80%-101.18%, and the relative standard deviations were between 0.29%-3.04%. These findings demonstrated the excellent detection performance of the proposed fluorescent aptamer sensor.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144246569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of MDM4 as a Prognostic Biomarker and a Target for Therapeutics in Colorectal Cancer.","authors":"Xiao Shang, Xiaoqiang Zheng, Aimin Jiang, Ni Zhao, Yuru Chen, Na Liu, Min Chen, Xuan Liang, Zhiping Ruan, Tao Tian, Xiao Fu, Yu Yao","doi":"10.1002/bab.70002","DOIUrl":"https://doi.org/10.1002/bab.70002","url":null,"abstract":"&lt;p&gt;&lt;p&gt;Colorectal cancer (CRC) is a serious global health problem. Even with improvements in CRC diagnosis and treatment, many patients are diagnosed with metastatic disease, indicating the tumor has metastasized, and the survival rate for those with advanced CRC is still low. Immune checkpoint inhibitors (ICIs) have shown some promise for certain groups of CRC patients, specifically for those with mismatch repair deficiencies or microsatellite instability, but their overall effectiveness is still limited. Novel biomarkers and treatment targets are critically needed for the improvement of the diagnosis and treatment of CRC, ultimately improving patient outcomes. MDM4 (murine double minute 4) protein is important in controlling the tumor suppressor p53. MDM4 is similar in structure to MDM2 and is known to block p53's transcriptional ability, which can contribute to tumorigenesis. MDM4 is often found at higher levels in many cancers, including CRC, and has been linked to cancer progression through mechanisms that don't involve p53. However, MDM4's role in the tumor immune microenvironment of CRC remains unclear; its role in CRC prognosis and response to immunotherapy isn't fully understood. This study explores the biological, clinical, and immunological impact of MDM4 in CRC, focusing on its potential as a marker for prognosis and treatment target. This study is the first to comprehensively link MDM4 overexpression in CRC to immune evasion through reduced infiltration of CD8+ T cells and dendritic cells, establishing its role as an independent prognostic marker and a potential immunotherapy target. We explored the role of MDM4 in CRC by combining bioinformatic analyses and laboratory experiments. We gathered data from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databasesWe performed Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, and Gene Set Enrichment Analysis (GSEA) to identify the key biological pathways linked to MDM4 in CRC. We also explored how MDM4 expression is associated with the immune microenvironment by examining the tumor-infiltrating lymphocytes in CRC tissues. Laboratory experiments were conducted to test the functional role of MDM4 in CRC cell lines. Our analysis showed that MDM4 expression was higher in CRC than in normal colorectal tissues, with even higher levels found in more advanced tumor stages. Increased MDM4 expression was linked to poorer progression-free survival (PFS) in CRC patients and was identified as an independent predictor of prognosis. Through pathway enrichment analyses, we found that MDM4 was involved in important tumor-related and immune pathways, including those regulating cell cycle progression and immune response. Notably, overexpression of MDM4 was associated with lower infiltration of CD8 T cells, natural killer (NK) cells, and dendritic cells in the tumor microenvironment, suggesting that MDM4 might help the tumor evade the immune system. In vitro","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144246570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Relationship Between Aldose Reductase and Isoxazole Derivatives: An In Vitro and In Silico Approach to Its Correlation With Diabetic Conditions.","authors":"Ahmet Esat Göner, Hatice Esra Duran","doi":"10.1002/bab.70003","DOIUrl":"https://doi.org/10.1002/bab.70003","url":null,"abstract":"<p><p>Diabetes mellitus (DM), which can result in a number of problems such as cataracts, neuropathy, retinopathy, nephropathy, and several cardiovascular illnesses, continues to be a growing issue despite major advancements in treatment approaches. Numerous scientists have targeted the polyol pathway as a target for intervention since it includes aldose reductase (ALR2, AR (E.C.1.1.1.21)), a crucial enzyme. Oxidative damage, NADPH depletion, and intracellular sorbitol buildup result from the overactivation of ALR2 brought on by hyperglycemia. Interest in creating novel ALR2 inhibitors (ALR2Is) with enhanced therapeutic characteristics has increased as a result of this circumstance. The amazing biological capabilities of isoxazole molecules led us to look into the biological properties of isoxazole and related compounds. We examined these isoxazoles' binding affinities and interactions in the ALR2 active site using thorough in vitro and in silico techniques. In comparison to the reference pharmaceutical epalrestat (EPR, K_I 232.70 ± 15.51 nM), our results demonstrate that these isoxazoles efficiently inhibit ALR2 at nanomolar doses, with inhibition constants (K_I) ranging from 12.13 ± 1.24 nM to 89.51 ± 4.68 nM. Important interactions between these isoxazoles and ALR2 are highlighted by the combined in vitro and in silico studies, indicating their potential as therapeutic agents against a range of pathological diseases. Furthermore, these substances that have ALR2 inhibitory properties could be useful as stand-in treatments or preventative measures for diabetes problems.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144233255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of Salvianolic Acid A-Loaded Gelatin Nanoparticles for Effective Suppression of Inflammation and Oxidative Stress in Blood-Brain Barrier to Ischemic Stroke Therapy.","authors":"Chaoming Li, Haixin Li, Haichun Zhou","doi":"10.1002/bab.2766","DOIUrl":"https://doi.org/10.1002/bab.2766","url":null,"abstract":"<p><p>Ischemic stroke (ICS) represents a treatment emergency for which efficient therapeutic options remain insufficient. Salvianolic acid A (SAA) is a naturally occurring polyphenol recognized as an effective neuroprotective agent. Compared with developed drugs, SAA demonstrates low side effects and displays several modes of action, providing considerable benefits in managing ICS. Yet, limitations of inadequate transmembrane permeability and water solubility hinder the effectiveness of SAA. Recently, nanodelivery methods have garnered significant attention in ICS as an efficient to penetrate the blood-brain barrier and enhance drug solubility. This investigation developed a new nanomedicine (SAA@GRH NPs) for ICS treatment, utilizing SAA-loaded gelatin nanoparticles (SAA@GNPs) that were functionalized and altered with brain tissue target rabies virus glycoprotein (RVG29). The stability, antioxidant, antibacterial, neuroprotective effects, cellular uptake, and cytocompatibility of SAA@GRH NPs were examined. The in vivo efficacy of SAA@GRH NPs on ICS was studied in a rat model of middle cerebral artery occlusion (MCAO) with histological analysis. The resultant SAA@GRH NPs enhanced the solubility of SAA and demonstrated effective dispersion. In vitro studies indicate that SAA@GRH NPs possess significant antibacterial activities, antioxidant capabilities, and protection against intracellular reactive oxygen species. RVG29 markedly improved the absorption of SAA@GRH NPs by IMR32 cells. Moreover, in vivo investigations confirmed the efficacy of SAA@GRH NPs in mitigating nerve injury and facilitating neurological recovery. In the MCAO model, SAA@GRH NPs markedly diminished neuroinflammation, substantially recovered behavioral functions and decreased neuronal death. Collectively, our data suggested that SAA@GRH NPs may offer an innovative and promising strategy for the successful treatment of ICS.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":"e2766"},"PeriodicalIF":3.2,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144214993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Purification and Characterization of Hyaluronidase From Streptomyces graminisoli.","authors":"Doha Nassir, Dalia A El-Ebeedy, Osama A M Said, Omar S Keshk, Seham Sater Alhelaify, Ohud Muslat Alharthy, Dalal Sulaiman Alshaya, Ahmed Z Abdel Azeiz","doi":"10.1002/bab.2789","DOIUrl":"https://doi.org/10.1002/bab.2789","url":null,"abstract":"<p><p>Hyaluronidases (HAase) are a family of hydrolytic enzymes that degrade hyaluronic acid (HA) and have gained significant attention in medical and pharmaceutical applications. This study presents an optimized approach for isolating and characterizing a novel HAase-producing actinomycete strain. Using HA-supplemented minimal salt medium (MSM) as the sole carbon and nitrogen source, we isolated and identified Streptomyces graminisoli by 16S-rRNA sequencing. The enzyme was purified using an improved DEAE-Sephadex G50 column chromatography method, yielding a molecular weight of 35 kDa. Kinetic analysis results revealed a K_m value of 0.28 mg/mL and a V_max value of 24.9 U/mL, indicating high substrate affinity. The results of advanced bioinformatics analysis using both nucleotide (Basic Local Alignment Search Tool nucleotide [BLASTN]) and amino acid (Basic Local Alignment Search Tool protein [BLASTP]) sequences demonstrated significant conservation across actinomycetes, with sequence identity and query coverage exceeding 75% and 90%, respectively. This study provides new insights into microbial HAase production and characterization, with potential applications in biotechnology and medicine.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144214994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Matrix Metalloproteinases 9 Contributes to Inflammatory Responses in Helicobacter pylori-Positive Gastric Cancer.","authors":"Wen-Jie Kong, Wei-Dong Liu, Man Wang, Wen-Jia Hui, Huan He, Lin Yu, Feng Gao","doi":"10.1002/bab.70000","DOIUrl":"https://doi.org/10.1002/bab.70000","url":null,"abstract":"<p><p>Helicobacter pylori-induced inflammatory microenvironment plays a pivotal role in the development of gastric cancer (GC). This study investigates whether matrix metallopeptidase 9 (MMP9) mediates H. pylori-induced effects in GC progression. GC patients were stratified on the basis of H. pylori infection status. MMP9 levels were measured using enzyme-linked immunosorbent assay (ELISA), immunohistochemistry, and RT-PCR. AGS and MKN45 GC cell lines were transfected with MMP9-specific siRNA and cocultured with H. pylori. Cell viability, proliferation, and migration were assessed using 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay, colony formation assay, and transwell migration assay, respectively. Expression of pro-inflammatory cytokines in cells and their secretion into coculture supernatants were analyzed by Western blotting and ELISA. MMP9 levels were significantly elevated in the serum of H. pylori-positive GC patients compared to H. pylori-negative patients. Correspondingly, increased MMP9 mRNA and protein expression were detected in H. pylori-positive GC tissues. Serum levels and tissue mRNA expression of interleukin (IL)-1β, tumor necrosis factor (TNF)-α, and IL-8 positively correlated with MMP9 expression. In vitro, H. pylori infection enhanced MMP9 expression and promoted the secretion of IL-1β, TNF-α, and IL-8 in AGS and MKN45 cells. H. pylori stimulation also increased the survival, proliferation, and migration of GC cells, which were significantly attenuated by MMP9 knockdown. MMP9 mediates H. pylori-induced inflammatory responses that contribute to the GC progression. Targeting MMP9 may represent a therapeutic strategy to counteract H. pylori-driven GC development.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144183593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}