Biotechnology and applied biochemistry最新文献

{"title":"Alcanivorax: Unique Genus of Hydrocarbon-Degrading Bacteria.","authors":"Nurdan Gönül Baltacı, Kübra Nur Bayındırlı, Mustafa Özkan Baltacı","doi":"10.1002/bab.70048","DOIUrl":"https://doi.org/10.1002/bab.70048","url":null,"abstract":"<p><p>Natural seepage, anthropogenic activities and accidents affect the ecosystem by increasing hydrocarbon footprints in the environment and cause a disruption in the biogeochemical balance. In addition, these imbalances result in human diseases and a decrease in the diversity of animals and microorganisms. Microbial bioremediation is the only sustainable option for the cleanup of hydrocarbon-impacted wastes, and the genus Alcanivorax is famous for its extraordinary ability to degrade hydrocarbons. The remarkable capacity of the Gram-negative bacterial genus Alcanivorax to break down a variety of hydrocarbons, including long-chain n-alkanes, has attracted a lot of attention in the field of environmental biotechnology. Because they can effectively use petroleum hydrocarbons as their only carbon and energy source, these bacteria are very well-suited for bioremediation and contribute significantly to the natural mitigation of oil spills and other hydrocarbon contaminants. To the best of our knowledge, this is the first comprehensive review specifically addressing the taxonomy, genomic features, and hydrocarbon degradation mechanisms of the genus Alcanivorax.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145013762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biotechnological Application of New Cold-Active Protease From Aeromonas salmonicida subsp. salmonicida EDT1.","authors":"Ezgi Dag Taskesenligil, Esra Aygun, Sumeyye Akbulut, Melda Sisecioglu, Ahmet Adiguzel","doi":"10.1002/bab.70045","DOIUrl":"https://doi.org/10.1002/bab.70045","url":null,"abstract":"<p><p>This study involved the isolation of ten psychrophilic bacterial strains from cold water in Söğütlü village, Erzurum. Following isolation, the strains were characterized using molecular and conventional methods. On the basis of the results of Petri dish assays, Aeromonas salmonicida subsp. salmonicida EDT1 (GenBank accession no: PP068881) exhibited the highest protease activity. The cold-active protease obtained from A. salmonicida subsp. salmonicida EDT1 was partially purified using a one-step, three-phase partitioning (TPP) method under the following conditions: pH 9.0; a ratio of crude extract to t-butanol of 1.0:1.5; and 80% saturated ammonium sulfate. This resulted in a yield of 244% and a purification fold of 42. The molecular weight of the enzyme was found to be approximately 39.44 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis. The optimal pH and temperature for the protease were 9.0 and 5°C, respectively. Although enzymatic activity increased after 60 min at 5°C, it gradually declined thereafter. Protease activity increased in the presence of Mg²⁺ (1 mM), Na⁺ (5 mM), and Mn²⁺ (10 mM) by 253%, 213%, and 169%, respectively. Phenylmethylsulfonyl fluoride (PMSF) significantly inhibited the enzyme, reducing its activity to 15%. After 1 h of incubation, activity increased in the presence of 50% acetone and 50% isopropanol to 393% and 256%, respectively. SDS increased protease activity by 336%. The enzyme exhibited a K_m of 0.751 mg/mL and a V_max of 43.29 µmol/mL/min for casein. The enzyme retained substantial activity after exposure to various commercial detergents. Purified EDT1 protease effectively removed wet and dried blood, as well as grass stains. The enzyme-detergent combination was most effective after 1 h of incubation.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145013808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of the EPIQ Quality Improvement Method on Extrauterine Growth Restriction in Preterm Infants: A Before-After Observational Study.","authors":"Yamei Su, Jianfang Ge, Yongping Xu","doi":"10.1002/bab.70047","DOIUrl":"https://doi.org/10.1002/bab.70047","url":null,"abstract":"<p><p>A common problem among preterm newborns is extrauterine growth restriction, or EUGR. The Evidence-based Practice for Improving Quality (EPIQ) strategy aims to reduce EUGR and enhance growth outcomes in neonatal intensive care units (NICUs). The objective of this study is to assess whether implementing EPIQ-based quality improvement interventions is associated with reduced EUGR among preterm infants (< 34 weeks gestation) in a before-after observational study. This study used a before-after design, analyzing retrospective baseline data and prospective postintervention data. A total of 817 preterm infants were included: 231 in the control group (admitted between January 1, 2022, and June 30, 2022; data collected retrospectively) and 586 in the experimental group (admitted between July 1, 2022, and December 31, 2023; data collected prospectively) at Shanxi Children's Hospital. The impact of the interventions was assessed using chi-square and t-tests. There was no significant difference in maternal conditions across groups (p > 0.05). The overall incidence of EUGR was significantly lower in the experimental group (37.54%) than in the control group (57.14%) (p < 0.01). Breast milk usage increased from 25.97% to 41.12% (p < 0.05) and human milk fortifier use increased from 9.09% to 28.84% (p < 0.01), indicating significant improvements in average length and weight growth in the experimental group (p < 0.05). Implementation of EPIQ-based interventions was associated with a significant reduction in EUGR incidence and improved growth outcomes in preterm infants under 34 weeks, supporting its role in enhancing neonatal care.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145013815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Berberine Alleviates Chronic Restraint Stress-Induced Depression-Like Behavior by Modulating Gut Microbiota and SCFA Production in Mice.","authors":"Ru Cheng, Aoqi Song, Jingjing Jiang, Xijier Qiaolongbatu, Zhenghua Wu, Feng Qian, Shuyu Shen, Liwen Zhang, Zhiyu Wang, Wenjuan Zhao, Yuefen Lou","doi":"10.1002/bab.70043","DOIUrl":"https://doi.org/10.1002/bab.70043","url":null,"abstract":"<p><p>The exact mechanism by which berberine alleviates depression remains unclear. In this study, we explored the relationship between the antidepressant effect of berberine and the microbiota-brain-gut axis. The levels of IL-1β, TNF-α, IL-6, corticosterone, serotonin, and brain-derived neurotrophic factor (BDNF) were quantified using enzyme-linked immunosorbent assays (ELISA) and Western blotting. Variations in the composition of the gut microbiota were examined using 16S rRNA gene sequencing. Berberine significantly mitigated depressive behaviors in mice with CRS, as manifested by increased total distance traveled and central zone duration in the open-field examination, increased time and number of entries into the outstretched arms during the elevated and maze tests, and an increase in the exercise time during the tail suspension and forced swimming tests. Histopathological analysis indicated that berberine ameliorated CRS-induced hippocampal and colonic damage in mice. Additionally, berberine substantially restrained the generation of proinflammatory cytokines and corticosterone in mice with CRS, while increasing the levels of BDNF and serotonin. Importantly, berberine significantly ameliorated CRS-induced depression-like behaviors (p < 0.01) and restored gut microbial diversity and short-chain fatty acid (SCFA) levels (fold-change: acetate 1.8-fold, butyrate 2.2-fold; p < 0.05). Furthermore, berberine restored the CRS-induced alterations in SCFA production. Our results indicate that berberine may exert antidepressant effects via a pleiotropic mechanism that modulates the microbiome-brain-gut axis.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144943688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioprocess Engineering Strategies for the Overproduction of Surface-Expressed Protein in Escherichia coli: A Review.","authors":"Fatemeh Poureini, Valiollah Babaeipour, Reza Hasan Sajedi, Rasoul Khalilzadeh","doi":"10.1002/bab.70034","DOIUrl":"https://doi.org/10.1002/bab.70034","url":null,"abstract":"<p><p>The use of whole cells represents a modern approach to enzymatic bioconversion for the production of various compounds, particularly pharmaceuticals. In recent decades, the use of wild strains as whole-cell biocatalysts has faced limitations due to challenges such as the lack of control over enzyme production and activity, as well as inefficiencies in enzyme production. As a result, recombinant cells are often employed. Among these, Escherichia coli is the most widely preferred bacterial host for producing recombinant proteins, thanks to its rapid growth, well-developed molecular manipulation tools, the ability to achieve high cell density using cost-effective culture components, and desirable genetic stability. The surface expression of enzymes is one of the most appropriate ways to increase the biotransformation efficiency by recombinant E. coli and reduce overall production costs due to the elimination of the need to purify enzymes and perform the enzyme conversion process in the presence of the pure substrate dissolved in the buffer. This article provides a thorough review of the various factors that influence the production of recombinant surface proteins. It examines aspects that affect biomass growth and methods to enhance protein expression. Additionally, recent research achievements in increasing the production of surface proteins are highlighted, along with promising insights that could pave the way for more sustainable and efficient approaches to producing surface-expressed proteins.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144943683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TCS-TP: Transporter Protein Prediction Based On Multi-Scale Feature Extraction.","authors":"Fan Yu, Qianying Zheng, Qingwei Fu, Jiansen Chen","doi":"10.1002/bab.70041","DOIUrl":"https://doi.org/10.1002/bab.70041","url":null,"abstract":"<p><p>Transporter proteins play a crucial role in maintaining ionic homeostasis inside and outside the cell, facilitating protein uptake, and enabling cellular communication with the external environment. Transporter proteins (TPs) are also significant targets for medical research and drug development. Accurately predicting novel TPs remains a major challenge in functional genomics. In this study, we propose TP prediction models, designated as TCS-TP, that utilize transformer and convolutional neural networks for multi-scale feature extraction of protein sequences. The transformer incorporates the GLU activation function, whereas the convolutional neural network (CNN) features three parallel subnetworks. Support vector machines are used as a classifier for TP classification. The test results demonstrate that TCS-TP could successfully recognize TPs, with AUROC of 0.89, AUPRC of 0.81, and accuracy of 91.6617%. Upon further comparison, it is determined that TCS-TP outperforms other methods. We hoped that TCS-TP will prove to be a valuable tool for predicting TPs in large-scale genomic projects and contribute to the discovery of new TPs.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144943745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High Expression of PDIA4 Contributes to Poor Prognosis in Glioma.","authors":"Yang Liu, Hua He, Yourui Zou, Jin Feng, Haibo Liu, Peng Gao, Wenchao Lu, Yang Zhao, Hui Ma","doi":"10.1002/bab.70042","DOIUrl":"https://doi.org/10.1002/bab.70042","url":null,"abstract":"<p><p>This study aimed to investigate the expression and prognostic value of protein disulfide isomerase A4 (PDIA4) in glioma patients. We initially analyzed the expression of PDIA4 in various tumors using the Gene Expression Profiling Interactive Analysis 2 (GEPIA2) database. Then, we performed a retrospective analysis of clinical and pathological data of 118 patients with glioma from January 2015 to January 2019 at the Department of Neurosurgery, General Hospital of Ningxia Medical University. Additionally, normal brain tissue samples were obtained from three patients undergoing surgery for intracerebral hemorrhage. Immunofluorescence was utilized to evaluate PDIA4 expression in gliomas and normal brain tissues, and we analyzed the correlation between PDIA4 expression, tumor malignancy grade, and survival prognosis. GEPIA2 dataset analysis showed significantly higher PDIA4 expression in most tumor tissues compared to normal tissues, particularly in gliomas. Immunofluorescence revealed PDIA4 localization, primarily in the cytoplasm of tumor cells. Amongst 118 patients, PDIA4 expression positively correlated with age (p < 0.005), WHO tumor grade (p < 0.0001), but showed no correlation with gender, tumor size, or tumor location. Notably, glioma patients with high PDIA4 expression demonstrated significantly lower overall survival rates than those with low expression (p < 0.0001), especially in low-grade glioma patients (p < 0.0001), while no significant correlation was found in high-grade glioma patients (p < 0.1356). Multivariate analysis revealed that elevated high PDIA4 expression is an independent prognostic factor associated with adverse outcomes in glioma patients (p < 0.0001). PDIA4 emerges as a novel molecular biomarker for prognostic prediction in glioma patients. Its elevated expression is associated with unfavorable outcomes, highlighting its potential as both a therapeutic target and a prognostic indicator in glioma management.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144943736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Signal Amplification to Improve Electrochemical Biosensing for Infectious Diseases.","authors":"Aarti Gupta, Shriyansh Srivastava, Pougang Golmei, Laxmi Yadav, Sachin Kumar","doi":"10.1002/bab.70044","DOIUrl":"https://doi.org/10.1002/bab.70044","url":null,"abstract":"<p><p>Infectious disease detection and monitoring are critical for public health management. Electrochemical biosensors have emerged as promising tools for rapid and sensitive detection of infectious diseases. This review explores signal amplification approaches to improve the sensitivity and limit of detection of electrochemical biosensors for infectious diseases. Enzymatic signal amplification methods, utilizing enzymes such as endonuclease, nucleotidyl transferase, DT-diaphorase, and alkaline phosphatase, are discussed along with examples of their application in detecting tuberculosis, HIV, and COVID-19. Nanoparticle-based amplification approaches, including gold nanoparticles, quantum dots, and magnetic nanoparticles, are explored, highlighting their utility in detecting hepatitis B, Zika virus, and Ebola virus. Additionally, label-free amplification techniques such as electrochemical impedance spectroscopy and surface plasmon resonance are examined, with examples demonstrating their efficacy in detecting dengue virus and influenza virus. Hybrid signal amplification methods combining enzymatic, nanoparticle-based, and label-free approaches are also discussed, showcasing their potential in detecting malaria and bacterial infections. Challenges such as the need for point-of-care testing and overcoming interferences are addressed, along with future research directions, including multiplexed assays and integration with smartphones for data analysis. This review provides insights into the diverse signal amplification strategies for electrochemical biosensors and their impact on infectious disease diagnosis and control.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144943757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of Antioxidant, Antimicrobial Potential, Soil Nutriology, and Molecular Docking Study of Bioactive Compounds of Eleusine coracana (Finger Millet), Pennisetum glaucum (Pearl Millet), and Sorghum bicolor (Sorghum).","authors":"Saikat Mazumder, Debasmita Bhattacharya, Dibyajit Lahiri, Debanjan Mitra, Moupriya Nag","doi":"10.1002/bab.70038","DOIUrl":"10.1002/bab.70038","url":null,"abstract":"<p><strong>Background: </strong>Being nutricereals with recognized nutritional content, bioactive components, and therapeutic prospects, millets support agronomic relevance and dietary value objectives of sustainable development.</p><p><strong>Method: </strong>The present study investigated soil health and nutriology, phyto-compounds (gas chromatography-mass spectrometry [GC-MS] profiling), proximate composition, the total phenolic content (TPC), total flavonoid content (TFC), DPPH (2,2-diphenyl-1-picrylhydrazyl), and H₂O₂ (peroxide) scavenging activity, antimicrobial potential, of Eleusine coracana, Pennisetum glaucum, and Sorghum bicolor cultivated in Panchal, Bankura, West Bengal, India.</p><p><strong>Key results: </strong>The soil texture is sandy clay loam in nature, and iron content was found maximum among the micronutrients present in the soil. The E. coracana and P. glaucum contain the maximum carbohydrate and protein content, respectively. GC-MS profiling millets detected the presence of hydrocarbon, fatty acid, triterpene, fatty amide, and sterol compounds. Sorghum exhibited total phenolic and flavonoid content with 231 mg GAE/100 g and 186.79 mg quercetin equivalents (QE)/100 g, respectively. However, E. coracana shows 16.04% and 23.85% more H₂O₂ scavenging activity than sorghum and P. glaucum, respectively, and more DPPH scavenging activity at 91.02% at a concentration of 36 µg/mL. Ethanolic extract of sorghum showed antimicrobial efficacy against Escherichia coli (ATCC 25922), Staphylococcus aureus (ATCC 6538), and Pseudomonas aeruginosa (ATCC 10145) with the zone of inhibition of 2.4 ± 0.12, 2.6 ± 0.12, and 2.1 ± 0.12 cm, respectively. The minimum inhibitory concentration (15 µg/mL) of ethanolic extract of sorghum exhibited inhibition of S. aureus by 92.64% ± 2.01%, P. aeruginosa by 96.64% ± 3.02%, and E. coli by 98.64% ± 3.02%. Further, molecular docking study found cis-stilbene with LasR protein from P. aeruginosa, Lupeol, methyl ester with SarA protein from S. aureus, 1,3-docosenamide, (Z)- with CpxR from E. coli as effective.</p><p><strong>Conclusion: </strong>Sorghum exhibited maximum phenolic, flavonoid content, and antimicrobial potential against P. aeruginosa (ATCC 10145). Sorghum bioactive compounds exhibited more efficiency than ciprofloxacin in biofilm eradication, rhamnolipid, pyocyanin, and elastase activity reduction in P. aeruginosa (ATCC 10145). cis-stilbene exhibited maximum binding efficacy (-8.2 kcal/mol) against the LasR protein from P. aeruginosa.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144871564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling the Complexities in TNBC Gene Interaction Networks Towards Better Therapeutics.","authors":"Priyanga Paranthaman, HemaNandini Rajendran Krishnamoorthy, Selvakumar Veluchamy, Ramanathan Karuppasamy, Shanthi Veerappapillai","doi":"10.1002/bab.70039","DOIUrl":"https://doi.org/10.1002/bab.70039","url":null,"abstract":"<p><p>Triple-negative breast cancer (TNBC) presents a substantial global health challenge due to its highly aggressive and invasive traits. To address this, the present study employed network-based analysis using protein-protein interaction (PPI) data to identify hub proteins and their functional insights. We constructed a PPI network consisting of 1413 dysregulated genes in TNBC. These hub genes displayed 5763 interactions. The downstream analysis identified TP53, SRC, EP300, AKT1, HSP90AA1, MAPK3, EGFR, and SMAD3 as hub proteins based on topological parameters and functional enrichment analyses. The DGIdb data were exploited to investigate the link between drugs and the identified hub genes. Collectively, the findings showed that no Food and Drug Administration (FDA)-approved drugs exist to target the EP300 protein. This situation necessitates the discovery of potential repurposed candidate against EP300 protein from the repository of 2147 FDA-approved compounds. Precisely, molecular docking, MM-GBSA, and ML-based rescoring analysis identified DB06137 (tirbanibulin) as a lead compound. Moreover, the dynamic behavior of DB06137 reinforces its exceptional structural stability with EP300, indicating its potential for novel therapeutic applications. We hypothesize that tirbanibulin interacts either with the regulatory regions of EP300 through allosteric inhibition or indirectly by altering upstream signaling pathways. Given the vital role of drug repurposing, experimental investigations on tirbanibulin unanticipated effects against EP300 are an interesting future direction.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144943765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}