Biotechnology and applied biochemistry最新文献

{"title":"Development of a Recombinant Chimeric Antigen Expressing Different Epitopes of Leishmania spp. For Diagnosis of Human Cutaneous Leishmaniasis","authors":"Mahmoud Nateghi-Rostami,&nbsp;Sahel Hamzehpour,&nbsp;Zohreh Askarypour,&nbsp;Jafar Amani,&nbsp;Jalal Babaie,&nbsp;Negin Elahirad","doi":"10.1002/bab.70159","DOIUrl":"10.1002/bab.70159","url":null,"abstract":"<div>\u0000 \u0000 <p>Development of highly sensitive and specific diagnostic tests for cutaneous leishmaniasis (CL) remains a significant challenge. Localized immune response in dermal leishmaniasis results in low systemic antibody production. Consequently, serologic diagnostic platform should focus on detecting <i>Leishmania</i> antigens directly from the skin lesions. Designing multiepitope-based proteins as an alternative to crude antigens can enhance sensitivity and specificity, lower production costs, and facilitate development of more field-amenable diagnostic tools. A chimeric multiepitope protein construct (THAG) was designed from four <i>Leishmania</i> spp. genes (<i>txnpx</i>, <i>gp63</i>, <i>hsp70</i>, and <i>amastin</i>). Bioinformatic tools were employed to ensure that the construct was optimized for expression in <i>Escherichia coli</i> and had favorable physicochemical properties. The gene was synthesized, subcloned into the pET28a(+) vector, and expressed in <i>E. coli</i> BL21 (DE3). Protein purification was carried out using Ni-NTA affinity chromatography under hybrid conditions. The purified protein was used to generate polyclonal antibodies in rabbits, and the immunoreactivity of antiserum was assessed against <i>Leishmania</i> antigens from lesions of 54 volunteers with suspected active skin lesions mimic CL through an in-house ELISA. The THAG multiepitope recombinant protein of <i>Leishmania</i> has successfully designed, expressed, purified and confirmed in our study. The results demonstrated sensitivity, specificity and accuracy of the ELISA test in detection of <i>Leishmania</i> antigens in CL lesions to be 90.91% (75.67%–98.08%), 95.24% (76.18%–99.88%), and 92.59% (82.11%–97.94%), respectively. This multiepitope protein has the potential to be further evaluated as a diagnostic tool for CL patients. While we have evaluated this multiepitope protein in an ELISA format, it might be applied to other test formats especially in immunochromatographic assays for the rapid detection of <i>Leishmania</i> antigens in skin lesions. Furthermore, it has the potential to be used as a tool to detect anti-<i>Leishmania</i> antibodies in serum samples of canine and human visceral leishmaniasis (VL) patients.</p>\u0000 </div>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":"73 5","pages":"2135-2148"},"PeriodicalIF":2.5,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147572478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Purification and Characterization of Amylase From Priestia megaterium SFA and Green Synthesis of Nanoparticles","authors":"Fatma Akbas Aydin,&nbsp;Murat Ozdal,&nbsp;Sumeyra Gurkok","doi":"10.1002/bab.70167","DOIUrl":"10.1002/bab.70167","url":null,"abstract":"<div>\u0000 \u0000 <p>This study focused on the isolation of amylase-producing bacteria from decaying potato peels, the identification of the isolate with the highest amylase activity, the characterization of the enzyme, and nanoparticle (NP) biosynthesis using culture supernatant. Among 40 isolates, <i>Priestia megaterium</i> SFA (GenBank Accession No.: OR415891.1) exhibited the highest amylase activity (0.76 U/mL). The enzyme was subsequently purified through ammonium sulfate precipitation, ultrafiltration, and anion exchange chromatography, achieving a 20.4-fold increase in purity, a 9.6% yield, and a specific activity of 10.2 U/mg. Biochemical characterization revealed a molecular weight of 57 kDa, a maximum reaction velocity (<i>V</i>_max) of 13.3 U/mg protein, and a Michaelis constant (<i>K_m</i>) of 4.5 mg/mL. The amylase exhibited optimal activity at pH 9.0 and 40°C and maintained stability in the presence of various metal ions, reagents, and organic solvents. Notably, the amylase-containing culture supernatant enabled the successful biosynthesis of copper (Cu) and magnesium (Mg) NPs, highlighting its potential as a biocatalyst for green nanotechnology applications. The synthesized NPs were confirmed and characterized using UV–visible spectroscopy, Fourier-transform infrared (FT-IR) spectroscopy, x-ray diffraction (XRD), scanning electron microscopy (SEM), and transmission electron microscopy (TEM) analyses.</p>\u0000 </div>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":"73 5","pages":"2223-2234"},"PeriodicalIF":2.5,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147580704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and Characterization of a Novel Microbacterium takaoensis Lipase for Nutritional and Textural Improvement of Pizza Dough.","authors":"Hetsi Goswami, Ashok Kumar Bishoyi, Gaurav Sanghvi","doi":"10.1002/bab.70187","DOIUrl":"10.1002/bab.70187","url":null,"abstract":"<p><p>Lipases are industrially important enzymes with broad applications in food processing, particularly in enhancing dough quality. In the current work, a lipase-producing strain was collected from oil-contaminated soil and recognized as Microbacterium takaoensis through 16S rRNA gene sequencing. Optimal lipase production was achieved under submerged fermentation using olive oil (2.0%) as the carbon and yeast extract (0.5%) as the nitrogen source at pH 7.0. The purification of lipase was carried out by ammonium sulfate precipitation along with size exclusion chromatography. Molecular weight by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis demonstrated the weight of lipase for approximately 40 kDa. Biochemical characterization showed that the lipase exhibited optimal activity at 37°C and pH 7.0 and was significantly enhanced by Zn²⁺, Fe²⁺, and Cd²⁺. Functional application studies of the purified lipase in pizza dough show improved nutritional content, increased protein and lipid levels, and reduced fiber content. Textural analysis revealed enhanced softness and elasticity, whereas scanning electron microscopy (SEM) imaging confirmed improved microstructural uniformity in treated dough. These findings suggest that M. takaoensis derived lipase holds strong potential for clean-label applications in functional food development.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":"2496-2509"},"PeriodicalIF":2.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148204339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel Inhibitors Targeting Class B1 Metallo-β-Lactamases in Carbapenem-Resistant Acinetobacter baumannii.","authors":"Mohanraj Gopikrishnan, George Priya Doss C","doi":"10.1002/bab.70180","DOIUrl":"10.1002/bab.70180","url":null,"abstract":"<p><p>The rise of multidrug resistance (MDR) in Acinetobacter baumannii has severely compromised the efficacy of carbapenem antibiotics. This resistance is primarily driven by class B1 metallo-β-lactamases (MBLs), including IMP, VIM, and NDM, which hydrolyze a broad spectrum of β-lactams, including carbapenems. To address this challenge, we conducted an extensive structure-based virtual screening campaign specifically targeting IMP-2, VIM-1, and NDM-1, integrating high-throughput docking, ADME/T filtering, and molecular dynamics validation to identify novel inhibitors. In total, 66,734 compounds from the ENAMINE, CMNPD, ASINEX, and ChemDiv libraries were evaluated, with marine-derived CMNPD molecules showing superior drug-likeness and binding potential. Virtual screening was performed using the Schrödinger suite, and top-ranked hits were validated using GROMACS-based molecular dynamics simulations, MM-GBSA binding free energy calculations, and essential dynamics analysis to confirm stability and binding efficiency. Three lead inhibitors, CMNPD29415 (IMP-2; XP G-score: -14.17 kcal/mol; MM-GBSA: -84.53 kcal/mol), CMNPD8077 (VIM-1; -11.76 kcal/mol; MM-GBSA: -50.11 kcal/mol), and BDE_30700625 (NDM-1; -13.80 kcal/mol), exhibited stable protein-ligand interactions and robust dynamic behavior. Key interactions were observed with catalytic residues, including GLU24, HIS35, GLU153, ASP224, and HIS240, supporting their inhibitory potential. MD trajectories revealed low RMSD fluctuations and reduced active-site mobility, further strengthening the therapeutic relevance of these compounds. Overall, this study highlights structurally unique marine metabolites as promising inhibitory scaffolds against Class B1 MBLs, and upcoming experimental validation will determine their translational applicability.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":"2388-2407"},"PeriodicalIF":2.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148013681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization and Characterization of Poly-γ-Glutamic Acid Production From a Locally Isolated Bacillus cereus ARM24.","authors":"Mohammed Jabbar Hafiz Al-Wahili, Alaa Jabbar Abd Al-Manhel, Rawdah Mahmood Al-Ali, Rawaa H Tlay, Tarek Gamal Abedelmaksoud","doi":"10.1002/bab.70189","DOIUrl":"10.1002/bab.70189","url":null,"abstract":"<p><p>For identification of this new local poly-γ-glutamic acid (γ-PGA) bacteria and partial purification of γ-PGA, the bacterium was studied and identified as Bacillus cereus. Sixty isolates of Bacillus spp. were collected from 28 sources (agricultural soil, local market vegetables, and fruits). Preliminary screening was performed on selective plates; 37 strains were selected on the basis of their ability to form a slimy mucoid colony. All isolates were studied for morphological and microscopic characters and identified by biochemical tests; a test for the production of γ-PGA was conducted with spectrophotometric determination of the polymer concentration at 216 nm. The best local strain B3, causing 2.54 g/mL of polymer, was chosen. Isolated strain was designated ARM24 and was published elsewhere with 99% identity as B. cereus ARM24 under the GenBank accession number. Optimization studies indicated that the optimum conditions for γ-PGA production were 37°C, pH 7, inoculum size 2 mL, shaking speed 200 rpm, and fermentation time 48 h using grape pomace juice as carbon source and soybean meal with salting out as nitrogen source. The polymer was precipitated from cold 99% ethanol with a recovery rate of 3:1, purified by dialysis bags (10,000 Da), and characterized by Fourier transform infrared (FT-IR), thin-layer chromatography (TLC), ¹H-NMR, and high-performance liquid chromatography (HPLC).</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":"2510-2531"},"PeriodicalIF":2.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148238877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeting Amastigote and Trypomastigote Phases: Multi-Epitope Vaccine Strategy Against Trypanosoma cruzi.","authors":"Maria Karolaynne da Silva, Caio Patrício de Souza Sena, Davi Serradella Vieira, Magdi E A Zaki, Leow Chiuan Yee, Shahina Akter, Umberto Laino Fulco, Jonas Ivan Nobre Oliveira, João Firmino Rodrigues-Neto","doi":"10.1002/bab.70185","DOIUrl":"10.1002/bab.70185","url":null,"abstract":"<p><p>Effective vaccines against Trypanosoma cruzi, the causative agent of Chagas disease, are urgently needed. Here, we report the design and in silico validation of a novel multiepitope vaccine construct targeting the key surface proteins ASP-2 and gp82. Using a comprehensive immunoinformatics pipeline, we identified and selected 38 potent T-cell (CTL/HTL) and B-cell epitopes, ensuring high antigenicity, immunogenicity, and safety. The final chimeric protein, integrated with adjuvants and stabilizing linkers, demonstrated favorable physicochemical properties, high solubility, and was predicted to be non-allergenic and non-toxic, with a significant population coverage of approximately 62% in Latin America. Structural modeling and refinement confirmed a stable tertiary structure. Crucially, molecular docking predicted high-affinity interactions with the immune receptors TLR2 and TLR4 (docking scores: -1360.4 and -1278.7, respectively). The stability and flexibility of these vaccine-receptor complexes were further validated by 300 ns molecular dynamics simulations. Finally, codon optimization and in silico cloning projected high expression potential in an Escherichia coli system. Immune simulations predicted robust responses: Innate (elevated cytokines, dendritic cell activation), humoral (IgG/IgM production), and cellular (CD4+/CD8+/memory T-cell activation) across simulated doses. These findings strongly support the potential of this vaccine candidate and provide a solid foundation for its further preclinical development against T. cruzi.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":"2457-2481"},"PeriodicalIF":2.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13644382/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148052867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Formulation of PLA/Chitosan Copolymeric Nanocarriers Enhanced the Sustained and Targeted Drug Delivery of Cinnamic Acid: In Vitro Drug Optimization and In Vivo Biodistribution in Rat Model.","authors":"Renuka Mani, Saranya Kannan, Kaviya Panner, Palvannan Thayumanavan","doi":"10.1002/bab.70184","DOIUrl":"10.1002/bab.70184","url":null,"abstract":"<p><p>Polymeric nanoparticles have emerged as attractive carriers for cancer therapies and brain-related disorders due to their increased permeability and retention (EPR) effect. Cinnamic acid (CA), a multifunctional bioactive molecule with notable antioxidant, anti-inflammatory, and anticancer activities, was encapsulated into a polymeric nanoparticle system composed of polylactic acid and chitosan. Comprehensive physicochemical characterization demonstrated effective CA loading in polymeric nanoparticles by displaying a transition from crystalline-to-amorphous, which improved solubility and bioavailability. The CA loaded polylactic acid/chitosan nanoparticles (CA-PLA/CS NPs) were spherical and homogenous in size (80-135 nm) with a positive zeta potential of +8.17 mV that ensured colloidal stability. The sustained and pH-responsive drug release suited to tumor microenvironments and managing brain-related disorders. CA-PLA/CS NPs preserved substantial antioxidant activity and had anti-inflammatory effects equivalent to diclofenac sodium and hemolysis were below the acceptable safety threshold. Animal studies confirmed that retained neural, hepatic and renal morphology with no damage, indicating systemic tolerance. Pharmacokinetic tests demonstrated that CA-PLA/CS nanoparticles had higher plasma concentrations than free CA, demonstrating the delivery system's efficacy. Overall, CA-PLA/CS NPs formulation syndicates structural stability, biocompatibility, and therapeutic efficacy, creating a strong platform for targeted cancer medication delivery and brain-related disorders.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":"2441-2456"},"PeriodicalIF":2.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147970362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Indigo Dye: From Ancient Extraction to Green Biomanufacturing.","authors":"Simeng Zhang, Xiaoyi Wang, Wei Chen, Jie Zheng, Chengtao Wang, Chenchen Qi, Guohui Chen, Lidan Niu","doi":"10.1002/bab.70161","DOIUrl":"10.1002/bab.70161","url":null,"abstract":"<p><p>Indigo, a historically significant blue dye widely used in textiles such as denim, was originally derived from plants. However, traditional extraction faced issues like low yield and high land use, while later chemical synthesis relied on toxic compounds, creating environmental and health concerns. Recently, microbial synthesis has emerged as a sustainable alternative, requiring only 10% of the time of plant-based methods and 80% less water than chemical production. Besides textiles, indigo is also applied in medicine, food, cosmetics, and semiconductors. This review covers the history, biosynthesis, detection, and diverse applications of indigo, addressing current challenges and highlighting its potential for eco-friendly dye manufacturing.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":"2532-2550"},"PeriodicalIF":2.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147763229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced Hypoglycemic Activity of Metformin with the Reduction of the Particle Size.","authors":"Moupiya Ghosh, Sayantan Pradhan, Sanjukta Dasgupta, Payel Das, Sumana Dutta, Sourajit Maity, Kartik Sau, Sanjib Ray, Swapan Kumar Pradhan","doi":"10.1002/bab.70181","DOIUrl":"10.1002/bab.70181","url":null,"abstract":"<p><p>The particle size of the antihyperglycemic drug metformin has been reduced to the nano range. The structural and microstructural characterizations of pure metformin and nano-metformin have been carried out by the Rietveld refinement of the XRD patterns and by analyzing UV-Vis absorption spectra. A docking study has also been performed to calculate the binding affinities of pure metformin and nano-metformin. To confirm the theoretical result of the docking study, an in vivo study has also been performed by administering both pure metformin and nano-metformin to 18-h-fasted normal mice. It has been confirmed that the FBGLs (fasting blood glucose levels) of mice have been reduced more in the case of nano-metformin compared to that of pure metformin. This in vivo result is in good agreement with the molecular docking result, which confirmed the higher binding efficacy of nano-metformin than that of pure metformin. This study proposes a new route to enhance the efficacy of a drug solely by reducing its particle size to the nanoscale without using any external agents.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":"2408-2418"},"PeriodicalIF":2.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147866678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Waste to Wealth: Tamarind Seed Dietary Fiber as a Potent Prebiotic Feed Additive.","authors":"Balaji Arumugam, Ranjita S Das, Shweta Deotale, Ipsita Chakravarty, Yogesh Ninawe, Praful Dadhe, Kuldeep Y Deshpande, Sachin A Mandavgane","doi":"10.1002/bab.70182","DOIUrl":"10.1002/bab.70182","url":null,"abstract":"<p><p>Tamarind (Tamarindus indica) seeds, often discarded as agro-industrial waste, are a rich source of bioactive compounds and dietary fibers. In this study, tamarind seed powder (TSP) was enzymatically treated for 4 h (e₄TSP), 6 h (e₆TSP), and 8 h (e₈TSP) to decipher its nutritional, functional, and bioactive properties. The e₆TSP contained protein (13.99 g/100 g) and carbohydrate content (64.77 g/100 g), with low moisture (3.12 g/100 g) and water activity (0.37), ensuring storage stability. Antioxidant evaluation revealed exceptionally high total phenolics (3506.78 mg GAE/100 g) and flavonoids (5877.6 mg QE/100 g), along with significant radical scavenging capacity. Fourier Transform Infrared Spectroscopy analysis confirmed the presence of polysaccharides, proteins, and lipid-associated functional groups. Enzymatic hydrolysis revealed substantial potential of prebiotic oligosaccharides (XOS, FOS, GOS, MOS), with maximum yields at 6 h. In vitro assays demonstrated that e₆TSP supported the growth of probiotic Lactobacillus spp. and significantly enhanced the production of short-chain fatty acids (SCFAs), including acetate, propionate, and butyrate. These dual effects probiotic stimulation and SCFA generation highlight e₆TSP as a potential stimbiotic ingredient. The findings position tamarind seed powder as a sustainable bioresource for food, nutraceutical, and poultry feed applications, while contributing to effective waste valorization.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":"2419-2432"},"PeriodicalIF":2.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147866928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}