Biotechnology and applied biochemistry最新文献_第4页

Yeasts as Biofertilizers and Biocontrol Agents: Mechanisms and Applications. 酵母作为生物肥料和生物防治剂：机理和应用。

IF 3.2 4区生物学

Biotechnology and applied biochemistry Pub Date : 2025-07-11 DOI: 10.1002/bab.70029

Olena Dmytruk, Alla Yemets, Kostyantyn Dmytruk

{"title":"Yeasts as Biofertilizers and Biocontrol Agents: Mechanisms and Applications.","authors":"Olena Dmytruk, Alla Yemets, Kostyantyn Dmytruk","doi":"10.1002/bab.70029","DOIUrl":"https://doi.org/10.1002/bab.70029","url":null,"abstract":"Yeasts, a large and diverse group of microorganisms, are gaining increasing interest from both the scientific community and industry. Yeasts' natural association with plants has endowed them with a broad repertoire of mechanisms that facilitate a beneficial coexistence. Despite the ability of certain yeast species to enhance plant growth and demonstrate broad-spectrum antifungal activities, their commercialization remains limited. This mini-review focuses on recent insights into the mechanisms by which yeasts stimulate plant growth and protect plants from certain diseases. Yeast species support plant growth by increasing the supply or availability of essential nutrients or by producing phytohormones. The mode of action of yeasts as biological control agents includes competition for nutrients and space, mycoparasitism, formation of biofilms that inhibit pathogen growth, and production of killer toxins, hydrolytic enzymes, and volatile organic compounds. Additionally, yeasts can induce systemic resistance in host plants by enhancing defensive enzyme activity or upregulating pathogenesis-related gene expression. This mini-review explores the current and future applications of yeasts as biofertilizers and biocontrol agents, emphasizing the metabolic engineering of yeast strains and the engineering of plant microbiomes.","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144607369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RadientFusion-XR: A Hybrid LBP-HOG Model for COVID-19 Detection Using Machine Learning. RadientFusion-XR：基于机器学习的新型冠状病毒检测混合LBP-HOG模型

IF 3.2 4区生物学

Biotechnology and applied biochemistry Pub Date : 2025-07-11 DOI: 10.1002/bab.70020

Greeshma K V, J Viji Gripsy

{"title":"RadientFusion-XR: A Hybrid LBP-HOG Model for COVID-19 Detection Using Machine Learning.","authors":"Greeshma K V, J Viji Gripsy","doi":"10.1002/bab.70020","DOIUrl":"https://doi.org/10.1002/bab.70020","url":null,"abstract":"The rapid and accurate detection of COVID-19 (coronavirus disease 2019) from normal and pneumonia chest x-ray images is essential for timely diagnosis and treatment. The overlapping features in radiology images make it challenging for radiologists to distinguish COVID-19 cases. This research study investigates the effectiveness of combining local binary pattern (LBP) and histogram of oriented gradients (HOG) features with machine learning algorithms to differentiate COVID-19 from normal and pneumonia cases using chest x-rays. The proposed hybrid fusion model \"RadientFusion-XR\" utilizes LBP and HOG features with shallow learning algorithms. The proposed hybrid HOG-LBP fusion model, RadientFusion-XR, detects COVID-19 cases from normal and pneumonia classes. This fusion model provides a comprehensive representation, enabling more precise differentiation among the three classes. This methodology presents a promising and efficient tool for early COVID-19 and pneumonia diagnosis in clinical settings, with potential integration into automated diagnostic systems. The findings highlight the potential of this hybrid feature extraction and a shallow learning approach to improve diagnostic accuracy in chest x-ray analysis significantly. The hybrid model using LBP and HOG features with an ensemble model achieved an exceptional accuracy of 99% for binary class (COVID-19, normal) and 97% for multi-class (COVID-19, normal, pneumonia), respectively. These results demonstrate the efficacy of our hybrid approach in enhancing feature representation and achieving superior classification accuracy. The proposed RadientFusion-XR model with hybrid feature extraction and shallow learning approach significantly increases the accuracy of COVID-19 and pneumonia diagnoses from chest x-rays. The interpretable nature of RadientFusion-XR, alongside its effectiveness and explainability, makes it a valuable tool for clinical applications, fostering trust and enabling informed decision-making by healthcare professionals.","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144607421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Whole Transcriptomic Identification and Characterization of Potential ceRNA Regulatory Networks in Perirenal Adipose Tissue of Type-2-Diabetic Mellitus SD Rats With Diabetic Nephropathy. 2型糖尿病SD大鼠伴糖尿病肾病肾周脂肪组织潜在ceRNA调控网络的全转录组学鉴定和表征

IF 3.2 4区生物学

Biotechnology and applied biochemistry Pub Date : 2025-07-10 DOI: 10.1002/bab.70005

Yang Li, Yan Zhao, Rou Shi, Yan Li, Yunyun Chen, Zhu Zhou, Huifang Li

{"title":"Whole Transcriptomic Identification and Characterization of Potential ceRNA Regulatory Networks in Perirenal Adipose Tissue of Type-2-Diabetic Mellitus SD Rats With Diabetic Nephropathy.","authors":"Yang Li, Yan Zhao, Rou Shi, Yan Li, Yunyun Chen, Zhu Zhou, Huifang Li","doi":"10.1002/bab.70005","DOIUrl":"https://doi.org/10.1002/bab.70005","url":null,"abstract":"This study induced diabetic nephropathy (DN) in rats, analyzing perirenal adipose tissue (PRAT) via whole transcriptome sequencing to identify key mRNAs in DN pathogenesis. Type-2 diabetes was induced in SD rats, evaluating metabolic and renal indicators. Whole transcriptome sequencing identified differentially expressed RNAs in PRAT. CeRNA networks, PPI networks, and ingenuity pathway analysis (IPA) revealed key mRNAs linked to physiological indicators in DN. This study explores correlations between mRNAs and health parameters, shedding light on the complex interplay in type-2-diabetes mellitus (T2DM)-induced nephropathy. SD rats with type-2 diabetes exhibited insulin resistance, elevated blood glucose, disrupted lipid metabolism, and renal dysfunction. PRAT weight was higher in T2DM rats, and immunohistochemistry revealed distinct renal injury. Transcriptome sequencing identified 476 DE-mRNAs, 79 DE-miRNAs, 200 DE-lncRNAs, and 10 DE-circRNAs. The lncRNA-miRNA-mRNA network comprised 159 lncRNAs, 62 miRNAs, and 138 mRNAs, whereas the circRNA-miRNA-mRNA network included 76 mRNAs, 27 miRNAs, and 10 circRNAs. Key mRNAs (Lpl, Elovl6, Dgat2, Acaca, and Acly) were associated with 10 classical pathways according to IPA. Notably, all key mRNAs showed a negative correlation with blood urea nitrogen (BUN), serum creatinine, proteinuria, LDL-C, triglycerides (TG), and total cholesterol (TC), and a positive correlation with urine creatinine and HDL-C. Our study successfully established a T2DM model in SD rats and identified five key mRNAs, elucidating the role of PRAT in DN. These findings lay a scientific foundation for future investigations into DN.","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144607368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bio-Designed Carboxymethyl Chitosan/Polyvinyl Alcohol Hydrogel Containing Alpha-Tocopherol as a Three-Dimensional Wound Dressing, In Vitro and In Vivo Study. 生物设计含α -生育酚的羧甲基壳聚糖/聚乙烯醇水凝胶三维创面敷料的体外和体内研究

IF 3.2 4区生物学

Biotechnology and applied biochemistry Pub Date : 2025-07-10 DOI: 10.1002/bab.70010

Ghazaleh Naghizadeh Hanjani, Shiva Irani, Fereshteh Sharifi, Seyed Mohammad Atyabi

{"title":"Bio-Designed Carboxymethyl Chitosan/Polyvinyl Alcohol Hydrogel Containing Alpha-Tocopherol as a Three-Dimensional Wound Dressing, In Vitro and In Vivo Study.","authors":"Ghazaleh Naghizadeh Hanjani, Shiva Irani, Fereshteh Sharifi, Seyed Mohammad Atyabi","doi":"10.1002/bab.70010","DOIUrl":"https://doi.org/10.1002/bab.70010","url":null,"abstract":"Chitosan-based hydrogels have highly attractive potential for designing wound dressing production. To promote wound healing, it is admirable to develop three-dimensional (3D) multi-biological functional polyvinyl alcohol/carboxymethyl chitosan (PVA/CMC) hydrogel. A 3D biocompatible PVA/CMC hydrogel was prepared using a freeze-drying method under both sterile and unsterile conditions, without the use of a chemical cross-linker. The alpha-tocopherol (αT) and glycerol (Gly) loaded into the hydrogel resulted in stability, porosity, and a controlled release profile coordinated with the degradation rate of the hydrogel. The PVA/CMC + αT/Gly hydrogel displayed facilitates cell attachment and cellular migration in vitro evaluations. The prepared hydrogels notably suppressed Escherichia coli and Staphylococcus aureus activities, correlating with loaded αT concentrations (200 and 400 U). In addition, the PVA/CMC + αT (200 U)/Gly10% and PVA/CMC + αT (400 U)/Gly10% hydrogels examined wound healing in full-thickness excisional wounds in rat models. The in vivo results indicated wound closure (αT-200 U: 78.7% and αT-400 U: 90.9% after 14 days), upregulating the gene expression (CYTOKERATIN, COLLAGEN TYPE Ι, and VEGF), and increasing protein levels (Pan-cytokeratin: 34.98% and VEGF: 41.72%). These findings highlight the porous structure of the PVA/CMC + αT (400 U)/Gly10% hydrogel as a promising novel wound dressing candidate with pro-regenerative properties.","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144607419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioreactor pH Control System Using Interval Type-2 Fuzzy PID Controller. 区间2型模糊PID控制的生物反应器pH控制系统。

IF 3.2 4区生物学

Biotechnology and applied biochemistry Pub Date : 2025-07-10 DOI: 10.1002/bab.70027

Songyang An, Haotian Xu, Jizheng Zhong, Xiao Li

引用次数: 0

Starch-Based Lactoperoxidase Immobilization on Metal Oxide Nanofılm: A Novel Approach for the Synthesis, Characterization, and Antibacterial Potential. 淀粉基乳酸过氧化物酶在金属氧化物上的固定化Nanofılm：一种合成、表征和抗菌潜力的新方法。

IF 3.2 4区生物学

Biotechnology and applied biochemistry Pub Date : 2025-07-10 DOI: 10.1002/bab.70032

Songul Bayrak, Hasan Ozdemir

{"title":"Starch-Based Lactoperoxidase Immobilization on Metal Oxide Nanofılm: A Novel Approach for the Synthesis, Characterization, and Antibacterial Potential.","authors":"Songul Bayrak, Hasan Ozdemir","doi":"10.1002/bab.70032","DOIUrl":"https://doi.org/10.1002/bab.70032","url":null,"abstract":"This study aims to develop a novel method for immobilizing lactoperoxidase (LPO) on starch-based metal oxide bionanofilms and evaluate its structural and antibacterial properties. LPO is an enzyme with broad-spectrum antimicrobial activity, and its stabilization is crucial for industrial applications. In this research, LPO was successfully immobilized onto starch and starch-based metal oxide films (starch@magnesium oxide (MgO), zinc oxide (ZnO), and copper oxide (CuO)) via surface adsorption and entrapment techniques. The immobilization was confirmed by Fourier transform infrared spectroscopy (FT-IR), x-ray diffraction (XRD), and field emission scanning electron microscopy (FE-SEM). The optimal pH for both free and immobilized LPO was determined to be 6. Although the free enzyme exhibited maximum activity at 40°C, the immobilized enzyme demonstrated increased thermal stability, maintaining optimal activity at 60°C. Furthermore, the initial activity of the immobilized LPO was 16.5%-19% after 60 min at 60°C, whereas the free enzyme completely lost activity within 5 min. Storage stability tests revealed that immobilized LPO maintained 27.7%-39.6% of its initial activity for 21 days at 4°C and 25°C, whereas 95% of the free LPO was lost under the same conditions. The antibacterial properties of the prepared bionanofilms' were evaluated against gram-positive and gram-negative bacteria. The starch@ZnO/LPO film exhibited significant antibacterial activity among the tested films. These findings indicate that the immobilization of LPO onto starch-based metal oxide bionanofilms enhances its stability and antibacterial efficiency, making it a promising candidate for biomedical and industrial applications.","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144607367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-Cell Transcriptomic Analysis Reveals Hair Cell-Specific Molecular Responses to Polystyrene Nanoplastics in a Zebrafish Embryo Model. 单细胞转录组学分析揭示了斑马鱼胚胎模型中毛细胞对聚苯乙烯纳米塑料的特异性分子反应。

IF 3.2 4区生物学

Biotechnology and applied biochemistry Pub Date : 2025-07-10 DOI: 10.1002/bab.70028

Hyeon Mi Sung, Seok Hyun Kim, Eun Jung Kwon, Sang Hwa Jeong, Hyun Min Lee, Yun Hak Kim, Chang Kyu Oh

{"title":"Single-Cell Transcriptomic Analysis Reveals Hair Cell-Specific Molecular Responses to Polystyrene Nanoplastics in a Zebrafish Embryo Model.","authors":"Hyeon Mi Sung, Seok Hyun Kim, Eun Jung Kwon, Sang Hwa Jeong, Hyun Min Lee, Yun Hak Kim, Chang Kyu Oh","doi":"10.1002/bab.70028","DOIUrl":"https://doi.org/10.1002/bab.70028","url":null,"abstract":"Polystyrene nanoplastics (PSNPs) have emerged as pervasive environmental pollutants with potential toxicological effects on aquatic ecosystems. Their small size, hydrophobicity, and structural stability enable penetration into biological tissues, inducing diverse toxic responses. This study investigates the physiological and molecular impacts of PSNPs on zebrafish embryos using single-cell RNA sequencing and phenotypic analyses. While PS-NP exposure at environmentally relevant concentrations caused no significant changes in survival or overt phenotypes, it led to alterations in cell type proportions and gene expression. Differentially expressed gene (DEG) analysis revealed the upregulation of genes such as col1a1a, fgfbp2b, cytl1, and fstl1a, which were validated in vivo. These genes are associated with extracellular matrix remodeling, immune regulation, and tissue repair, suggesting that PSNPs activate defensive and reparative mechanisms in response to environmental stress. These findings highlight the molecular and cellular responses to PSNP exposure in zebrafish embryos and underscore the importance of evaluating the ecological risks posed by nanoplastics.","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144607422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction of Hollow Mesoporous Selenium Loaded With Carboplatin and Clacked With Bevacizumab-Functionalized Albumin Nanoparticles for HER2+ Lung Cancer. 含卡铂和贝伐单抗功能化白蛋白纳米颗粒的中空介孔硒的构建用于HER2+肺癌。

IF 3.2 4区生物学

Biotechnology and applied biochemistry Pub Date : 2025-07-07 DOI: 10.1002/bab.70007

Weibin Luo, Zhengyuan Yang, Shihua Gao, Bin Peng

{"title":"Construction of Hollow Mesoporous Selenium Loaded With Carboplatin and Clacked With Bevacizumab-Functionalized Albumin Nanoparticles for HER2+ Lung Cancer.","authors":"Weibin Luo, Zhengyuan Yang, Shihua Gao, Bin Peng","doi":"10.1002/bab.70007","DOIUrl":"https://doi.org/10.1002/bab.70007","url":null,"abstract":"Lung carcinoma is one of the most severe types of cancer, characterized by a dismal prognosis and low overall survival rate, primarily due to late diagnosis and the ineffectiveness of standard treatments. Hence, this study aimed to develop bevacizumab (BZ)-functionalized albumin (BS)-clad, carboplatin (CP)-loaded hollow mesoporous selenium (HMS) nanoparticles (BBHMSC) for the targeted administration of HER2+ Calu-3 lung cancer. The structural properties of these nanoparticles were characterized using transmission electron microscopy (TEM). Cell viability was assessed using the 3-[4,5-dimethylthiazol-2-yl]- 2,5-diphenyltetrazolium bromide (MTT) assay. The images were obtained from fluorescence microscopy. The in vitro characterization of the pharmacological characteristics indicated that BBHMSC was uniformly disseminated, with a particle size of 171.52 ± 4.3 nm. In vitro release tests demonstrated that BBHMSC proficiently regulated the release of CP in the tumor microenvironment (elevated glutathione concentration), achieving a cumulative release rate of 87.1 ± 3.9% over 48 h. A cellular uptake assay validated that BBHMSC has an effective tumor-targeting capability. In vitro cell investigations, MTT studies, have shown that BBHMSC dramatically suppresses proliferation and growth. Further, BBHMSC enhances the apoptosis of HER2+ Calu-3 cells by fluorescence and flow cytometry methods. The data indicate that BBHMSC may be an effective targeted therapy for HER2+ti lung cancer.","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144574858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Computational Modeling and Validation of Predicted Diagnostic Peptides for Crimean-Congo's Disease. 克里米亚-刚果病预测诊断肽的计算建模和验证。

IF 3.2 4区生物学

Biotechnology and applied biochemistry Pub Date : 2025-07-07 DOI: 10.1002/bab.70026

Elijah Kolawole Oladipo, Sola Peter Ogunmodede, Gladys Ayodele Adigun, Mercy Temiloluwa Ogunlowo, Oluwatomi Jeremiah Dada, Enyo Othniel Friday, Isaiah Isiolaotan Iyanuoluwa, Jerry Ayobami Oluwasegun, Olawumi Elizabeth Akindiya

{"title":"Computational Modeling and Validation of Predicted Diagnostic Peptides for Crimean-Congo's Disease.","authors":"Elijah Kolawole Oladipo, Sola Peter Ogunmodede, Gladys Ayodele Adigun, Mercy Temiloluwa Ogunlowo, Oluwatomi Jeremiah Dada, Enyo Othniel Friday, Isaiah Isiolaotan Iyanuoluwa, Jerry Ayobami Oluwasegun, Olawumi Elizabeth Akindiya","doi":"10.1002/bab.70026","DOIUrl":"https://doi.org/10.1002/bab.70026","url":null,"abstract":"The absence of prompt and reliable diagnosis limits the eradication of Crimean-Congo's hemorrhagic fever (CCHF) caused by Hyalomma. Although there have been several recent developments in different points of care and diagnosis, there has been a major drawback caused by the commercial unavailability and limited accessibility of economical serosurveillance assays that pose significant challenges. The primary objective of this study is to predict and design a novel synthetic biomarker composed of immunodominant B- and T-cell lymphocytes (HTL) interleukin 10 (IL-10) epitopes using the antigenic, non-toxigenic, and nonallergenic glycoprotein, nucleocapsid protein, and RNA-dependent RNA polymerase (RdRp). Top highly ranked B-cell epitopes as well as IL-10 inducing epitopes were predicted, screened, selected, and linked with linkers. Then, utilizing a variety of web servers, the biomarker predictions of antigenicity (0.5594), allergenicity (nonallergic), solubility (protein-sol score: 0.623), and physiochemical properties (molecular weight of 56,004.26 Da, instability index of 28.33), secondary (SOPMA) and tertiary structures (AlphaFold2), 3D model refinement (GDT_HA score of 1.0000), and validations were done. Additionally, codon adaptation (G-C of 48.93%, CAI 1.0) and in silico cloning in the pcDNA3 vector were done. The molecular docking (HDock) of the final biomarker with the selected genes was done to see the binding affinity. The model had a docking score of -291.82, a confidence score of 0.9446, and a ligand RSMD of 66.24. For this multi-epitope design to be employed in commercial serodiagnostic kits, further experimental evaluation is required for its development.","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144574857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improved Lovastatin Production via Targeted Medium Supplementation in Aspergillus terreus. 通过在地曲霉中添加靶向培养基提高洛伐他汀产量。

IF 3.2 4区生物学

Biotechnology and applied biochemistry Pub Date : 2025-07-07 DOI: 10.1002/bab.70025

Maryam Javanpour, Ramtin Vamenani, Ethan Brister

{"title":"Improved Lovastatin Production via Targeted Medium Supplementation in Aspergillus terreus.","authors":"Maryam Javanpour, Ramtin Vamenani, Ethan Brister","doi":"10.1002/bab.70025","DOIUrl":"https://doi.org/10.1002/bab.70025","url":null,"abstract":"Lovastatin is a clinically crucial cholesterol-lowering agent produced by Aspergillus terreus; however, its commercial biosynthesis remains limited due to suboptimal fermentation conditions. This study addresses the challenge of evaluating how the supplementation of l-cysteine (l-Cys), linoleic acid (LA), and riboflavin (vitamin B2) individually, in dual combinations, and, as a triple formulation, affects both fungal biomass and lovastatin yield. Among individual supplements, LA had the most pronounced effect, increasing lovastatin production to 1892.43 mg/L, whereas l-Cys and B2 yielded 1600.66 and 1176.82 mg/L, respectively. Dual combinations, such as l-Cys + LA and LA + B2, produced higher titers (2300 and 2282.96 mg/L, respectively), leading to significant increases in yield per gram of biomass. The highest productivity was achieved with triple supplementation (115 mg/L l-Cys + 90 mg/L LA + 0.66 mg/L B2), resulting in 2373.99 mg/L lovastatin, a biomass of 8.03 g/L, and a yield of 295.65 mg/g biomass. Biomass measurements across all treatments indicated that although fungal growth was moderately enhanced, the increase in lovastatin production was disproportionately greater. This suggests that the supplements primarily stimulate metabolic flux toward polyketide synthesis. These findings provide a foundation for media optimization strategies to enhance industrial-scale lovastatin production through precise nutrient control.","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144574859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0