Rosmanol Suppresses Nasopharyngeal Carcinoma Cell Proliferation and Enhances Apoptosis, the Regulation of MAPK/NF-κB Signaling Pathway.

Abstract

Nasopharyngeal Carcinoma (NPC) is a major public health problem in endemic zones. NPC is correlated with substantial illness and death; thus, superior treatment is desired. Rosmanol (RM) is a phenolic diterpene antioxidant extracted from the medicinal herb Rosemary (Rosmarinus officinalis). RM has been investigated for its anti-inflammatory and anti-tumor properties by numerous signaling cascades. However, the fundamental anticancer latent mechanism of RM persists as unidentified. Hence, this present research proposes to search for the anti-cancer efficacy of RM on human NPC cells CNE2 using an in vitro approach. To assess the possible molecular mechanisms of proliferation, apoptosis, cell-cycle regulatory proteins, and MAPKs/NF-κB signaling of NPC cells were administered RM (20 and 30 µM) and assayed through MTT, DCFH-DA, Rh-123 staining, AO/EB, PI, Rh-123/DAPI merge form staining, RT-PCR, and Western blot. The result was recognized that RM could reduce NPC cell viability by elevated intracellular ROS, MMP damage, and generate apoptosis. RM inhibits the Cyclin-D1, Bax, TNF-α, and NF-κB, and induces BCl-2 analyzed via RT PCR. RM attenuates the cell cycle mechanism by repressing NPC cell cycle-related proteins: CDK4/CDK6, pRB, cyclin-D1, and MAPKs/NF-κB signaling. These data established that the MAPKs/NF-κB pathway is a potential target for the remedial action of RM. In summary, RM may be an effective conventional chemotherapy drug in preventing the progression of NPC.