Biotechnology and applied biochemistry最新文献

{"title":"Apelin-13 Inhibits the Adhesion of Monocytes to Endothelial Cells via the Gfi1/NF-κB Signaling Pathway.","authors":"Yujuan Wei, Zhihong Chen, Qi Zhao, Kaili Wang, Xiaojing Zhu, Guobing Zhou, Zhengke Yu","doi":"10.1002/bab.2746","DOIUrl":"https://doi.org/10.1002/bab.2746","url":null,"abstract":"<p><p>Oxidized low-density lipoprotein (ox-LDL)-induced endothelial dysregulation and the abnormal interaction between monocytes have been considered key risk factors for atherosclerosis. The study investigated the effects of apelin-13 on ox-LDL-induced endothelial dysfunction in human aortic endothelial cells (HAECs). Cells were stimulated with ox-LDL (100 mg/L), either alone or in combination with apelin-13 at concentrations of 3 and 6 µM. Multiple techniques, including real-time PCR, Western blot analysis, enzyme-linked immunosorbent assay (ELISA), cell attachment assays, and luciferase activity assays, were employed. Our results showed that ox-LDL reduced the expression of the G-protein-coupled apelin receptor (APJ) in HAECs. However, treatment with apelin-13 reduced the expression of lectin-like ox-LDL receptor 1 (LOX-1) against ox-LDL and inhibited the expression of pro-inflammatory cytokines in HAECs. Real-time PCR and ELISA assay demonstrated that apelin-13 also inhibited the expression of cell adhesion molecules intercellular cell adhesion molecule-1 (ICAM-1) and E-selectin. The calcein AM staining method displayed that apelin-13 mitigated ox-LDL-induced attachment of THP-1 monocytes to HAECs. Furthermore, apelin-13 prevented the reduction of growth factor independence-1 (Gfi1) and the activation of NF-κB in HAECs, as evidenced by the luciferase activity assay. Knockdown of Gfi1 counteracted the inhibitory effects of apelin-13 on the attachment of THP-1 monocytes to HAECs, suggesting that the protective effects of apelin-13 in endothelial dysfunction are mediated by the Gfi1/ NF-κB signaling pathway. These findings suggest that apelin-13 may have therapeutic potential in preventing atherosclerosis by improving endothelial function and reducing inflammation.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":"e2746"},"PeriodicalIF":3.2,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Invasive Weeds Lantana camara L. and Parthenium hysterophorus L. on the Secondary Metabolite Profiles of Okra (Abelmoschus esculentus L.).","authors":"Pratik Talukder, Mrinmoy Dasgupta, Aniket Hazra, Ayan Adhikari, Anushka Dutta, Snehika Sengupta, Milan Karmakar","doi":"10.1002/bab.2751","DOIUrl":"https://doi.org/10.1002/bab.2751","url":null,"abstract":"<p><p>The impact of two invasive weed species, Lantana camara and Parthenium hysterophorus, on the development and secondary metabolism of okra (Abelmoschus esculentus) is the focus of this study. We explored the potential role of secondary metabolites as antioxidants to mitigate the stress induced by these common weeds on okra plants with special emphasis on chlorogenic acid (CGA). The study has been done with six experimental setups. One setup includes okra grown alone, and another set consists of okra co-cultivation with L. camara, P. hysterophorus, and a combination of both. Each setup consisted of 30 plants of each species. After these, biochemical assays were done to measure the production of secondary metabolites, which include polyphenols, flavonoids, and antioxidants. They are all measured in response to weed-induced stress. Total polyphenols, flavonoids, and antioxidants displayed a significant increment in okra when co-cultivated with Lantana or Parthenium. Co-cultivation with both weeds presented synergistic negative effects, like that interaction is characterized by elevated markers of stress, such as reactive oxygen species (ROS) and malondialdehyde (MDA) levels, indicating the compounded adverse effect on the okra plants. Moreover, it points out the effect of the weed's presence on secondary metabolism in okra. Gene expression analysis of two genes, phenylalanine ammonia lyase (PAL) and hydroxycinnamoyl-CoA quinate hydroxycinnamoyl transferase (HQT), which are critical in the phenylpropanoid pathway, validated the role of secondary metabolites in weed-induced stress alleviation. High-performance liquid chromatography (HPLC) analysis shows that CGA has increased significantly in okra plants co-cultivated with weeds. It suggests a plant response to counteract weed-induced stress by enhancing CGA production. Therefore, the study shows the impact of weeds on stress indicators, such as ROS, RNS, and MDA. So, it is observed that stress levels are increased in okra in the weed's presence, as indicated by high lipid and proline content. These findings suggest practical strategies for weed management by enhancing secondary metabolism in crops to improve resilience and productivity under stress conditions caused by invasive weeds.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":"e2751"},"PeriodicalIF":3.2,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selection of DNA Aptamers Against Parathyroid Hormone for Electrochemical Impedimetric Biosensor System Development.","authors":"Reza Didarian, Saharnaz Bargh, Almina Gülerman, Veli Cengiz Özalp, Özcan Erel, Nimet Yildirim-Tirgil","doi":"10.1002/bab.2745","DOIUrl":"https://doi.org/10.1002/bab.2745","url":null,"abstract":"<p><p>This work presents the pioneering development of an aptamer-based electrochemical biosensor for real-time monitoring of parathyroid hormone (PTH) levels, with a focus on intraoperative assessment during parathyroid surgery. It introduces, for the first time, the selection and characterization of aptamers targeting distinct segments of the PTH peptide. The study demonstrates the feasibility and efficacy of the biosensing platform through a precisely designed experimental framework, including SELEX-based aptamer selection, aptamer-peptide interaction analysis, and biosensor fabrication. The SELEX process yields aptamers with notable binding affinities to different fragments of PTH, with the PTH (53-84) aptamer showing particularly sensitive binding to the hormone's C terminus, allowing for precise PTH analysis. Electrochemical characterization reveals significant changes in electrochemical impedance spectroscopy (EIS) signals upon exposure to varying PTH concentrations, highlighting the sensor's sensitivity and selectivity. The increase in charge transfer resistance (Rct) values with rising PTH concentrations underscores the biosensor's capability to detect PTH-induced structural changes, validating its potential for accurate measurement. The biosensor shows remarkable selectivity in the presence of common interferents in serum samples, ensuring precise PTH detection. Stability assessments over a 45-day storage period demonstrate the biosensor's robustness and long-term reliability, affirming its practical suitability. In summary, the developed aptamer-based biosensor represents a promising tool for sensitive and selective PTH detection, with potential applications in biomedical research and clinical diagnostics, particularly for intraoperative PTH analysis during parathyroidectomy. Continued research and optimization efforts hold promise for enhancing its performance and expanding its utility in diverse healthcare settings.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":"e2745"},"PeriodicalIF":3.2,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnosis Accuracy of Raman Spectroscopy in the Identification of Pathogenic Bacteria.","authors":"Shanshan Mao, Yu Zhang, Chaoqun Chen, Liu Cong, Zuobin Zhu, Zhiyu Xie, Ying Li","doi":"10.1002/bab.2741","DOIUrl":"https://doi.org/10.1002/bab.2741","url":null,"abstract":"<p><p>As an emerging technology, Raman spectroscopy (RS) has been used to identify pathogenic bacteria with excellent performance. The aim of this study was to verify the diagnosis accuracy of RS in identification of pathogenic bacteria. This meta-analysis systematically evaluated the accuracy of RS for identification of pathogenic bacteria. We searched the electronic databases of PubMed and Web of Science to obtain relevant articles; STATA 15.1 was used to analyze all sensitivities, specificies, and their 95% confidence interval (CI). The summary receiver operating characteristic curves (SROC) and area under the curve (AUC) were used to display more performance of RS. Nineteen articles were included according to the inclusion and exclusion criteria. The pooled sensitivity and specificity of RS for the identification of pathogenic bacteria were 0.94 (95% CI, 0.89-0.96) and 0.99 (95% CI, 0.97-0.99). The diagnostic odds ratio (DOR) was 1209 (95% CI, 367-3980), and AUC of SROC was 0.99 (95% CI, 0.98-1.00). For gram-positive bacteria, the sensitivity and specificity of different species ranged from 0.00 to 1.00 and 0.96 to 1.00, with a pooled sensitivity and specificity of 0.96 (95% CI, 0.90-0.98) and 0.99 (95% CI, 0.98-1.00). For gram-negative bacteria, the sensitivity and specificity of different species ranged from 0.30 to 1.00 and 0.92 to 1.00, with a pooled sensitivity and specificity of 0.92 (95% CI, 0.76-0.98) and 0.99 (95% CI, 0.98-1.00). For acid-fast bacteria, the sensitivity and specificity of different species ranged from 0.83 to 1.00 and 0.96 to 1.00, with a pooled sensitivity and specificity of 0.96 (95% CI, 0.84-0.99) and 1.00 (95% CI, 0.96-1.00). RS provides a new method for pathogenic bacteria identification and demonstrates high sensitivity and specificity for most included species.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":"e2741"},"PeriodicalIF":3.2,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143622987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RETRACTION: Downregulation of SDCBP Inhibits Cell Proliferation and Induces Apoptosis by Regulating PI3K/AKT/mTOR Pathway in Gastric Carcinoma","authors":"","doi":"10.1002/bab.2733","DOIUrl":"10.1002/bab.2733","url":null,"abstract":"<p><b>RETRACTION</b>: B. Qian, Z. Yao, Y. Yang, N. Li, and Q. Wang, “Downregulation of SDCBP Inhibits Cell Proliferation and Induces Apoptosis by Regulating PI3K/AKT/mTOR Pathway in Gastric Carcinoma,” <i>Biotechnology and Applied Biochemistry</i> 69, no. 1 (2022): 240–247, https://doi.org/10.1002/bab.2103.</p><p>The above article, published online on 1 January 2021 in Wiley Online Library (wileyonlinelibrary.com), has been retracted by agreement between the journal Editor-in-Chief, Kaiming Ye; the International Union of Biochemistry and Molecular Biology; and John Wiley &amp; Sons. The retraction has been agreed due to concerns raised by third parties. Specifically, the article reports experiments in a non-verifiable cell line, BGC-803. Further investigation by the publisher has found that the article has been accepted solely based on the compromised peer review. Parts of the experimental methods in this manuscript lack sufficient detail to interpret and reproduce the findings. Additionally, the following cell lines mentioned and/or used in this study were found to be problematic/contaminated: BGC-823 [<span>1, 2</span>], MKN-28 [<span>3</span>] and SGC-7901 [<span>1, 2</span>]. Accordingly, the conclusions of this article are considered invalid by the editors and the article must be retracted. The authors were contacted but did not respond regarding the decision to retract.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":"72 2","pages":"570"},"PeriodicalIF":3.2,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bab.2733","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143603953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Apelin-13 Alleviates Diabetes-Associated Cognitive Decline by Reducing Oxidative Stress and Mitochondrial Dysfunction via the SIRT3/Foxo3 Pathway.","authors":"Shanshan Hu, Chaoyang Lan, Shengnan Shu, Lu Wang","doi":"10.1002/bab.2743","DOIUrl":"https://doi.org/10.1002/bab.2743","url":null,"abstract":"<p><p>The incidence of diabetes-related cognitive dysfunction is on the rise, yet clinical interventions to prevent this condition remain limited. Apelin-13, an endogenous peptide known for its positive inotropic and vasoactive properties, has been shown to exert diverse effects across various tissues and cell types. However, its potential protective role in diabetes-associated cognitive decline (DACD) remains poorly understood. To investigate this, we established a rodent diabetes model using a high-fat diet (HFD) combined with streptozotocin (STZ, intraperitoneal injection, 60 mg/kg). Cognitive function was evaluated using the Morris water maze and Y-maze tests. Additionally, we employed a range of techniques, including intraperitoneal glucose tolerance tests (IPGTT), insulin tolerance tests (ITT), immunofluorescence labeling, real-time PCR, Western blot analysis, and enzyme-linked immunosorbent assays (ELISA). Our results demonstrate that apelin-13 administration alleviated diabetes symptoms in the diabetic mouse model. Specifically, apelin-13 improved cognitive performance in both the Y-maze and Morris water maze tests. In the hippocampus of treated mice, apelin-13 reduced oxidative stress by enhancing the activity of superoxide dismutase (SOD) and catalase (CAT), while decreasing levels of malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE). Furthermore, apelin-13 improved mitochondrial function in the hippocampus by restoring the activities of COX I and COX IV (but not COX II) and increasing ATP production. Apelin-13 also restored SIRT3 expression and elevated the NAD+/NADH ratio in the hippocampus. As a result, apelin-13 facilitated the deacetylation and nuclear translocation of Foxo3a in the hippocampus. When SIRT3 was silenced, the beneficial effects of apelin-13 on oxidative stress, mitochondrial function, and cognitive impairment in diabetic mice were significantly diminished, underscoring the critical role of SIRT3 in these processes. In summary, our findings suggest that apelin-13 mitigates DACD by reducing oxidative stress and mitochondrial dysfunction through the SIRT3/Foxo3 pathway. These results highlight apelin-13 as a promising therapeutic candidate for DACD.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":"e2743"},"PeriodicalIF":3.2,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143603950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fabrication of Polypyrrole-Polystyrene Sulfonate by Amperometric Biosensor-Based Electropolymerization for Cholesterol Determination in Serum.","authors":"Alaa Anwer Ali Dada, Ertan Yildirim, Sinan Mithat Muhammed, Servet Çete","doi":"10.1002/bab.2742","DOIUrl":"https://doi.org/10.1002/bab.2742","url":null,"abstract":"<p><p>Since cholesterol triggers many diseases, many methods have been developed for its determination. In this study, an alternative system for cholesterol determination was developed by preparing amperometric biosensors. In the development of the biosensor, Pt/polypyrrole-polystyrene sulfonate film was prepared by electropolymerization of polypyrrole in polystyrene sulfonate medium using platinum surfaces. The cholesterol oxidase enzyme was immobilized on the prepared platinum/polypyrrole-polystyrene sulfonate electrode. For molecular determination in the prepared cholesterol biosensor, a series of enzymatic reactions were performed on the enzyme electrode surface at +0.40 V by utilizing the oxidation of hydrogen peroxide. The effects of environmental conditions such as temperature and pH that affect the performance of the biosensor were investigated, and the most suitable conditions for the biosensor were determined. The linear working range of the amperometric biosensor for cholesterol determination was determined. In enzyme immobilization, calculations were made for the Michaelis-Menten constants K_m and V_max values. Storage life and reproducibility of cholesterol biosensor were determined. Cholesterol determination in biological fluid (blood) was performed with the prepared biosensor. The fact that the fabricated amperometric-based cholesterol biosensor can be used for the diagnosis of many diseases is important in terms of early diagnosis in the future.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":"e2742"},"PeriodicalIF":3.2,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143603952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fabrication of Bioconjugates Clacked Chitosan-Coated SN-38 Liposomal Nanoparticles: Improved Precise Chemotherapy Efficacy in Lung Cancer Cells and Its Apoptosis.","authors":"Xiaojun Zhang, Wei Sun, Changting Liu","doi":"10.1002/bab.2740","DOIUrl":"https://doi.org/10.1002/bab.2740","url":null,"abstract":"<p><p>Employing an active targeting method with monoclonal antibodies for chemotherapeutics-loaded nanocarriers represents a promising option to enhance the specific drug delivery and alleviate the detrimental effects of chemotherapeutic agents. Targeted delivery to the human epidermal growth factor receptor-2 (HER2), which is overexpressed in HER2+ lung cancerous cells, can be accomplished by conjugating nanoparticles with a monoclonal antibody (anti-HER2). We developed trastuzumab (TZ)-conjugated chitosan iodoacetamide (CsIA)-coated liposomal nanoparticles carrying SN-38 (TZ-SN-CsIA LNPs) as a lung-targeted delivery. CsIA was used to develop trastuzumab-clacked nanoparticles (TZ LNPs). The structure, physicochemical characteristics, SN-38 encapsulation, SN-38 release, and anticancer properties of the LNPs were established. The TZ LNPs were spherical, measuring around 77 nm in diameter, and exhibited a positive zeta potential; upon drug incorporation, the diameter of the TZ LNPs enlarged. A sustained, 24-h SN-38 release from the nanocarriers was accomplished. TZ LNPs demonstrated substantial cellular accumulation and markedly enhanced anticancer efficacy against HER2+ Calu-3 lung adenocarcinoma cancer cells compared to the drug solution and unconjugated LNPs. Consequently, TZ-SN-CsIA LNPs may be a potential nanocarrier for HER2+ lung cancer.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":"e2740"},"PeriodicalIF":3.2,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143603951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research Progress on Biosynthesis and Regulation of Conjugated Linoleic Acid in Lactic Acid Bacteria.","authors":"Xin-Xin Liu, Selar Izzat, Guang-Qiang Wang, Zhi-Qiang Xiong, Lian-Zhong Ai","doi":"10.1002/bab.2737","DOIUrl":"https://doi.org/10.1002/bab.2737","url":null,"abstract":"<p><p>Conjugated linoleic acid (CLA) has gained considerable attention and has been extensively studied owing to its physiological functions, including weight loss, immune regulation, and anti-carcinogenesis. However, the CLA content is very low in natural sources and insufficient to exert a probiotic effect. Additionally, its chemical synthesis is limited by the lack of isomer selectivity, harsh reaction conditions, and the introduction of organic solvents. Therefore, the synthesis of CLA by microorganisms, especially lactic acid bacteria, holds great promise because of its isomer selectivity, green process, and probiotic effects of lactic acid bacteria. This article reviews the CLA biosynthesis abilities and mechanisms of CLA producers, particularly lactic acid bacteria. In addition, the factors that impact the CLA yield are discussed. Special attention has been paid to the regulation of CLA synthesis, including the optimization of culture media and conditions, co-culturing of different strains, and regulation of the activity and expression of CLA-related enzymes. Furthermore, prospective future research directions for CLA biosynthesis regulation are discussed.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143499458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plasma Exosomal circRNAs as Potential Biomarkers for Patients with Septic Shock-Induced Acute Kidney Injury.","authors":"Hailing Yang, Yan Li, Kuo Qu, Chunmei Zhang, Mei Ding, Yang Liu","doi":"10.1002/bab.2736","DOIUrl":"https://doi.org/10.1002/bab.2736","url":null,"abstract":"<p><p>Sepsis-induced acute kidney injury (AKI) is marked by high mortality and poor prognosis. Recent studies suggest that exosomal circRNAs regulate key pathways involved in inflammation, oxidative stress, and apoptosis, processes central to sepsis-induced AKI. This study investigates the differential expression of exosomal circRNAs and their functional implications in the pathogenesis of sepsis-induced AKI. Patients with sepsis-induced AKI and healthy volunteers were recruited for the research. Whole blood samples were collected, and exosomes were isolated and characterized through particle detection, transmission electron microscopy, and Western blot for exosomal marker proteins. Real-time PCR was used to analyze exosomal circRNAs, including circ-Fryl, circ-TLK1, circ-PTK2, circ-Ttc3, circ-VMA21, and circ_0091702. Additionally, assays were performed to assess cell proliferation, apoptosis, and uptake in HK-2 cells exposed to exosomes from both control and sepsis-induced AKI samples. Seven patients with sepsis-induced AKI and six healthy individuals were enrolled. Exosomal protein markers were analyzed from plasma samples of both groups. Exosomes from the sepsis-induced AKI group (mean particle diameter: 146.1 nm) and the control group (mean particle diameter: 150.9 nm) exhibited a characteristic spherical morphology. Plasma levels of circ-Ttc3 (p < 0.05), circ-Fryl (p < 0.01), and circ_0091702 (p < 0.01) were notably lower in the sepsis-induced AKI group relative to the control group. Additionally, HK-2 cells exposed to sepsis-induced AKI exosomes showed upregulation of circ-Ttc3 and downregulation of circ-TLK1. Following exposure to sepsis-induced AKI exosomes, HK-2 cell viability decreased while apoptosis increased. circRNAs such as circ-Ttc3, circ-TLK1, circ-Fryl, and circ_0091702 hold potential as candidate biomarkers and therapeutic targets for sepsis-induced AKI.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143490769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}