Biotechnology and applied biochemistry最新文献

{"title":"Production and Formulation of Alcanivorax borkumensis SK2 Cell Powders for Marine Oil Spill Bioremediation.","authors":"Élisabeth Perreault, Denis Groleau, Patrick Vermette","doi":"10.1002/bab.70009","DOIUrl":"https://doi.org/10.1002/bab.70009","url":null,"abstract":"<p><p>Oil spills pose severe threats to marine ecosystems and coastal communities. Alcanivorax borkumensis SK2, a marine bacterium with superior hydrocarbon-degrading capabilities, emerges as a promising agent for bioremediation. This study identified an economical growth substrate for A. borkumensis SK2 and led to highly viable cell powder formulations for effective applications in contaminated marine environments. Various non-hydrocarbon substrates were evaluated to replace the costly sodium pyruvate, revealing that canola oil and sunflower oil gave biomass levels (optical density) four times higher than sodium pyruvate (20 ± 2 and 20 ± 1, compared to 4.6 ± 0.4, respectively). Freeze-drying and spray-drying approaches were investigated to produce a viable cell formulation. Two screening campaigns of potential freeze-drying cryoprotectants showed that the proprietary blend of Proventus Bioscience Inc. (Proventus) and 0.5 M glutamate ensured the highest viability, with 2 ± 1×10¹⁰ and 1.1 ± 0.3 × 10¹⁰ CFU/g, after the first screening, and 1.0 ± 0.5 × 10¹⁰ and 6 ± 2 × 10⁹ CFU/g after the second screening. Differential scanning calorimetry (DSC) analysis demonstrated a 9%-15% reduction in ice formation with cooling rates from 5 to 10°C/min. Glutamate reduced ice formation by 5%-9% compared to Proventus' solution. To promote cell viability during A. borkumensis SK2 freezing and freeze-drying, the best product temperatures were determined to be -65°C with 0.5 M glutamate and -59°C with Proventus' blend. Spray-drying resulted in cell powders with a viability up to 1.0 ± 0.7 × 10⁵ CFU/g, considerably lower than the levels obtained by freeze-drying, indicating some potential but also the need for further research and optimization.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144574860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TIGAR Alleviates Acute Pancreatitis by Suppressing Glycolysis Through the LAMP2/PI3K/Akt Axis.","authors":"Dan Xie, Chiwen Bu","doi":"10.1002/bab.70022","DOIUrl":"https://doi.org/10.1002/bab.70022","url":null,"abstract":"<p><p>Acute pancreatitis (AP) is a prevalent inflammatory disorder of the pancreas, with emerging evidence implicating aberrant acinar cell glycolysis in disease progression. Although TIGAR (TP53-induced glycolysis and apoptosis regulator), a key glycolytic regulator, has been implicated in various pathological processes, its role in AP remains unexplored. In this study, we established an AP mouse model through intraperitoneal injection of high-dose caerulein. AP mice exhibited downregulated pancreatic TIGAR expression accompanied by enhanced glycolysis. In vitro, primary pancreatic acinar cells were stimulated with LPS to mimic the inflammatory microenvironment. TIGAR overexpression effectively mitigated LPS-induced reductions in cell viability, inflammatory cytokine expression, reactive oxygen species (ROS) production, and glycolytic activation. Notably, LAMP2 (lysosome-associated membrane protein 2) knockdown abolished the protective effects of TIGAR against LPS-induced ROS, inflammation, and glycolytic flux. Mechanistically, TIGAR suppressed LPS-induced glycolytic activation by upregulating LAMP2 expression, thereby inhibiting PI3K/Akt pathway activation. Consistently, the glycolytic inhibitor 2-DG reversed the detrimental effects of TIGAR knockdown on cell viability and inflammatory responses. Furthermore, both preventive and therapeutic AAV8-TIGAR administration attenuated AP progression in mice. In conclusion, our findings demonstrate that TIGAR protects against AP progression by modulating the LAMP2/PI3K/Akt axis to maintain glycolytic homeostasis, highlighting its potential as a therapeutic target for AP.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144574861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lactobacillus Cocktail Potentially Alleviated the Toxic Effects of Zearalenone in Mice Receiving a Zearalenone-Contaminated Diet.","authors":"Delaram Hassani, Golnar Kermani, Fatemeh Samizadeh Mashhadi, Behnoosh Taghizadegan, Sayna Moslemian Abbasabadi, Negin Shafaei, Ehsan Karimi, Ehsan Oskoueian","doi":"10.1002/bab.2782","DOIUrl":"https://doi.org/10.1002/bab.2782","url":null,"abstract":"<p><p>Zearalenone (ZEN) is a prevalent immunosuppressive and estrogenic metabolite synthesized by certain species of Fusarium fungi. This mycotoxin has a propensity to contaminate food and feed products, leading to immune system suppression and reproductive disorders. Therefore, the mitigation of ZEN production and the amelioration of its toxic effects are critical components in the management of ZEN contamination. This study aims to evaluate the Lactobacillus cocktail as a potential probiotic to reduce the toxicity of ZEN in mice receiving ZEN-contaminated food. Twenty-four mice were randomly divided into three groups for the study. The control group (T1) received a standard diet, whereas Groups T2 and T3 were given diets contaminated with ZEN at 10 µg/g. Additionally, Group T3 received a Lactobacillus cocktail at 10⁹colony-forming units (cfu)/g. ZEN levels were confirmed using liquid chromatography-mass spectrometry (LC-MS). On Day 28, the mice were sacrificed, and blood, liver, and ileum samples were analyzed. According to the findings, the Lactobacillus cocktail effectively decreased the ZEN concentration in vitro by up to 64.5% (p < 0.05). Mice that consumed a meal contaminated with ZEN showed impairment in their daily weight, food intake, liver enzyme, lipid peroxidation, intestinal morphometric traits, and gene expression. Dietary inclusion of Lactobacillus cocktail as a potential probiotic significantly improved these parameters (p < 0.05). The Lactobacillus cocktail likely mitigated the toxic effects of ZEN by enhancing immune responses, improving antioxidant capacity, and reducing the bioavailability of ZEN in the intestine through non-covalent binding to the toxin.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144567179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunoinformatics-Guided Whole Proteome-Based Multi-Epitope mRNA Vaccine Design Against Nocardia asteroides Using Surface Antigens-A Subtractive Proteomics and Reverse Vaccinology Approach.","authors":"Abdul Malik, Abbas Ahmad, Sara Aiman, Samavia Farrukh, Sabiha Fatima, Azmat Ali Khan","doi":"10.1002/bab.70018","DOIUrl":"https://doi.org/10.1002/bab.70018","url":null,"abstract":"<p><p>Nocardiosis is caused by the aerobic gram-positive bacterium Nocardia asteroides which is multidrug-resistant and primarily affects immunocompromised individuals. This study aims to design a broad-spectrum vaccine against Nocardiosis. We used subtractive proteomics and vaccinomics approaches to identify vaccine candidates to design an epitope-based vaccine against Nocardiosis. Four proteins in the outer membrane and extracellular regions were shortlisted on the basis of immunogenic parameters. T- and B-cell epitopes were predicted on the basis of IC₅₀ < 200 nM, and a multi-epitope vaccine construct was designed using six overlapping CTL, HTL, and B-cell epitopes conjugated by appropriate linkers. A highly immunogenic adjuvant was integrated at the N-terminus of the multi-epitope vaccine to stimulate a robust immune response. The tertiary structure of the vaccine construct was predicted and validated with the 96% residues in the favorite region of the Ramachandran plot and a Z-score of -4.76. The proposed vaccine exhibited strong immunological and physicochemical features. High docking scores and strong binding energies of approximately -1051.9 to -1274.5 kcal/mol with human immune receptors ensured that the designed vaccine construct could induce potential immunogenic responses in the host immune system. The efficacy of the vaccine was evaluated by the immune simulation to determine the potential of the vaccine to simulate innate and adaptive immunity with the development of long-lasting memory immune cells. The molecular dynamic simulation of a 100 ns study was conducted to determine the structural stability and molecular function of the designed vaccine in the cellular microenvironment. In silico restriction cloning analysis determined the successful expression of the engineered vaccine construct in Escherichia coli plasmid with a size of 6323 bp. On the basis of the current study, we assume that the proposed vaccine is worthy of further in vitro in vivo and in vivo validations to ensure the efficacy of the engineered vaccine in this study.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144526496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Co-Expression of Chaperones for Improvement of Recombinant Geobacillus stearothermophilus Lipase Production in Pichia pastoris.","authors":"Fidan Erden-Karaoglan","doi":"10.1002/bab.70017","DOIUrl":"https://doi.org/10.1002/bab.70017","url":null,"abstract":"<p><p>Pichia pastoris is among the most popular expression systems for recombinant protein production. The ability to secrete high titers of recombinant proteins is one of its primary advantages. Co-expression of folding-assisting factors is considered one of the strategies to improve protein production with this expression system. In this study, the effect of PDI, KAR2, HAC1, and ERO1 co-expression on Geobacillus stearothermophilus lipase production in P. pastoris was investigated. The chaperones were co-expressed under the regulation of the GAP promoter in clones with different lipase production levels (LPS#2 and LPS#8). Results showed that lipase-PDI co-expression clones have the highest activity. The effect of the other chaperones had varying effects for LPS#2 and LPS#8 clones. LPS#2-PDI and LPS#8-PDI were further analyzed to determine the effect of dimethyl sulphoxide (DMSO). Different concentrations (0.5%, 1%, and 2%) of DMSO were tested. The highest activity was obtained with approximately 1.5-fold activity in the LPS#8-PDI clone with 0.5% and 1% DMSO concentration levels. Comparison of the fermentation kinetic parameters revealed that the activity level of 56.54 U/mL provided with LPS#8 increased to 73.84 U/mL in the LPS#8-PDI clone with PDI co-expression, and when PDI co-expression was combined with DMSO conditions (0.5%), it reached 114.46 U/mL. It was observed that the productivity (U/g wet cell/g substrate/h) of the LPS#8 clone was increased 1.27-fold with LPS#8-PDI and 2.05-fold with LPS#8-PDI-DMSO conditions. In future studies, it is possible to achieve higher protein production levels by optimization at the fermenter scale.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144526494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Deubiquitinating Enzyme USP46 Abolishes Cell Survival of Prostate Cancer via Enhancing BECN1-Dependent Autophagy.","authors":"Yantao Yang, Hongwen Cao, Peng Sun, Yigeng Feng, Lei Chen, Renjie Gao","doi":"10.1002/bab.70016","DOIUrl":"https://doi.org/10.1002/bab.70016","url":null,"abstract":"<p><p>Ubiquitin-specific peptidase 46 (USP46) is frequently underexpressed in several cancers. Its involvement and functional role in prostate cancer, however, are not well understood. This research focused on exploring how USP46 may suppress prostate cancer and its underlying mechanisms. USP46 mRNA levels in clinical prostate cancer samples were analyzed using the online The University of Alabama at Birmingham Cancer Data Analysis Portal (UALCAN) database. The expression of USP46 mRNA and protein was analyzed using RT-PCR and immunoblotting techniques. Cell proliferation, colony formation assays, and tumor growth curves were assessed by Cell Counting Kit-8 assay, crystal violet staining, and xenograft model, respectively. USP46's interaction with beclin 1 (BECN1) was evaluated using immunoprecipitation assays, whereas BECN1 knockdown was performed with siRNA. USP46 mRNA expression was significantly lower in prostate cancer cells compared to that in normal cells. Overexpression of USP46 significantly reduced cell proliferation, as shown by decreased cell viability and fewer colonies. Mechanistic studies demonstrated that USP46 interacted with and stabilized BECN1, supported by findings showing that USP46 overexpression increased BECN1 levels and delayed its degradation in prostate cancer cells treated with cycloheximide. Additionally, knockdown of BECN1 and inhibition of autophagy partially restored cell proliferation in USP46-overexpressing cells. USP46 interacts with and stabilizes BECN1, promoting autophagy and thereby suppressing prostate cancer cell proliferation. In conclusion, the current findings demonstrate that USP46 abolishes cell survival of prostate cancer via enhancing BECN1-dependent autophagy.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144526497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Amylase From Fruit and Vegetable Waste: Its Immobilization and Application in the New Trend-A Review.","authors":"Rima Paul, Jyotchna Gogoi, Nayan Talukdar","doi":"10.1002/bab.70012","DOIUrl":"https://doi.org/10.1002/bab.70012","url":null,"abstract":"<p><p>Amylases function as hydrolytic enzymes, facilitating the decomposition of starch molecules and other associated polymers. These enzymes are found ubiquitously across all domains of life. Amylase dominates the enzyme market in terms of sales because of its extensive utilization in the starch processing field and its wide-ranging applications across the food, textile, and pharmaceutical sectors. Microorganisms are primarily used to produce amylase; they are readily available, flexible, and easy to employ. Fruit and vegetable wastes (FAVWs) containing proteins and lipids add to the detrimental effects on the environment. However, this waste offers cost-effective alternatives for manufacturing value-added products through the synthesis of industrially essential enzymes by microorganisms. The most recent advancements in biocatalytic systems aim to improve the catalytic efficiency of commercially available enzymes or generate new enzymes with unique features. This study emphasizes the valorization of FAVW to derive amylase, recent advancements in the use of enzyme immobilization approaches for sustainable development, and their application in the present scenario.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144526493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Coculture Models of Eukaryotic Cells With Pathogenic and/or Commensal Bacteria for Tissue Engineering","authors":"Olga Buchar Klinovská,&nbsp;Andrea Vávrová,&nbsp;Veronika Benson,&nbsp;Marie Hubálek Kalbáčová","doi":"10.1002/bab.2717","DOIUrl":"10.1002/bab.2717","url":null,"abstract":"<p>Appropriate <i>in vitro</i> systems are needed to evaluate effective strategies to prevent implant-associated infections, which remain a significant complication in today's medicine. Conventional <i>in vitro</i> models involving monocultures cannot fully reflect the tissue environment <i>in vivo</i>. Coculturing eukaryotic and prokaryotic pathogenic and commensal cells in <i>in vitro</i> models configured according to the needs of each tissue could better mimic complex tissue structures and actual clinical conditions. This approach may also enable better investigations of cell–cell interactions and interactions with an implant surface than culturing each cohort separately. This review aims to provide an overview of current studies and techniques that demonstrate competitive colonization between mammalian and bacterial cell populations (pathogenic and commensal), highlight the gaps in current techniques, and speculate on the future of using these complex cell systems in the field of tissue engineering and regenerative medicine.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":"72 4","pages":"1110-1120"},"PeriodicalIF":2.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://iubmb.onlinelibrary.wiley.com/doi/epdf/10.1002/bab.2717","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144526495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Methyl Gallate: A Potent Bioactive Compound Promoting Osteogenic Differentiation by Regulating Runx2 and Cbfa1 in Osteoblastic Cell Lines.","authors":"Neha Verma, Bimalkumar Patel, Bhawna Sharma, Shilpa Kumari, Showket Hussain, Hitesh D Patel, Kanu Priya","doi":"10.1002/bab.2784","DOIUrl":"https://doi.org/10.1002/bab.2784","url":null,"abstract":"<p><p>Osteoporosis is a widespread condition, particularly affecting women and high-aged groups, with limited allopathic treatment options leading to adverse effects. The interest in natural remedies for osteoporosis is growing, and plant-based compounds are being explored for their potential to promote bone regeneration. Vachellia nilotica, known for its various medicinal properties, has shown promise in traditional medicine but lacks scientific evidence for its role in osteoporosis treatment. This study focused on isolating from V. nilotica leaves and characterizing a compound, methyl 3,4,5-trihydroxybenzoate (methyl gallate), and its action upon osteoblastic cell lines to deal with osteoporotic disease. Methyl gallate is a phenolic compound. Methyl gallate is a substance that is used for many functions in the body; for example, it is used as an anti-inflammatory, an antioxidant, a neuroprotector, a hepato-protector, and an immunomodulator, as well as leading a range of research and development activities. The compound exhibited no cytotoxic effects at low concentrations on the MG 63 cell line, indicating its safety. It also demonstrated significant antioxidant activity, with the ability to scavenge radicals and reduce oxidative stress. Moreover, the isolated compound showed a stimulating effect on alkaline phosphatase activity, a marker of early differentiation of osteoblasts, thereby decreasing the activity of osteoclasts and thus leading to increased mineralization. The above results suggest the compound's potential to promote bone regeneration and healing. Overall, this study provides valuable insights into the compound's osteogenic differentiation properties from V. nilotica leaves and their potential as a natural remedy for osteoporosis.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144494682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polyphenols as Mitigators of Metal-Induced Stress in Madagascar Periwinkle (Catharanthus roseus L.).","authors":"Pratik Talukder, Mrinmoy Dasgupta, Aniket Hazra, Swastika Mohanta","doi":"10.1002/bab.70021","DOIUrl":"https://doi.org/10.1002/bab.70021","url":null,"abstract":"<p><p>Understanding the mechanisms by which plants respond to oxidative stress is crucial for ensuring agricultural sustainability. Plant growth and development get severely affected from the stress induced by environmental factors. Heavy metals like lead (II) nitrate are one of the major causes of abiotic stress. Oxidative stress can be experienced by plants due to the presence of an excessive amount of reactive oxygen species (ROS), causing adverse effects to the cellular components. Plants produce an increased number of antioxidants as part of their secondary metabolism as a direct response to the stress. Polyphenols and flavonoids are among the largest families of secondary metabolites having antioxidant properties, capable of sequestering the ROS in plants, reducing the stress levels. These polyphenols are produced in the phenylpropanoid pathway where phenylalanine lyase (PAL) acts as the very first enzyme initiating the pathway, the gene expression of which was studied by real-time PCR. Assays to assess the extent of the antioxidant properties were also carried out for biomarkers of stress, including lipid peroxidation, chlorine, and proline content. HPLC was performed to identify and quantify the various sub-families of polyphenols being produced in response to the stress. This research article provides an in-depth analysis of the impact of lead (II) nitrate induced stress in Catharanthus roseus L., renowned for its pharmaceutical significance, and the findings from this multi-faceted study offer a holistic understanding about the effects of lead (II) nitrate stress induced up-regulation of the synthesis of secondary metabolites, possessing antioxidant characteristics.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144494683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}