Biotechnology and applied biochemistry最新文献

{"title":"Enhanced Production of Astaxanthin and Zeaxanthin by Paracoccus Sp. LL1 Through Random Mutagenesis.","authors":"Anoth Maharjan, Beom Soo Kim","doi":"10.1002/bab.70019","DOIUrl":"https://doi.org/10.1002/bab.70019","url":null,"abstract":"<p><p>Astaxanthin, a high-value carotenoid with potent antioxidant and anti-inflammatory activities, is increasingly in demand in various industries. This study reports the successful enhancement of astaxanthin and zeaxanthin production in Paracoccus sp. LL1 through random mutagenesis using ethyl methanesulfonate (EMS). To induce genetic diversity, EMS mutagenesis was employed, followed by the selection of mutants that exhibited increased carotenoid production. The top-performing mutants showed a significant 2.76-fold increase in astaxanthin and a 10.14-fold increase in zeaxanthin compared to the wild-type strain when treated with the optimal EMS concentration of 0.5%. The effects of initial glucose concentration and inoculum size on astaxanthin and zeaxanthin production were evaluated, and production was higher when glucose was 2% and inoculum size was 10%. Our findings demonstrate the potential of Paracoccus sp. LL1 as a promising alternative to traditional astaxanthin-producing organisms, such as Haematococcus pluvialis, offering advantages including faster growth, simpler cultivation requirements, and genetic tractability. This approach not only enhances carotenoid production but also highlights the novelty of using Paracoccus sp. LL1, a less-explored strain, for high-yield production of both astaxanthin and zeaxanthin. These results suggest that Paracoccus sp. LL1 could serve as an efficient platform for industrial-scale carotenoid production through metabolic engineering and random mutagenesis, providing a viable alternative to current production systems.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144494713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Purification and Biochemical Characterization of a New Thermostable and Detergent-Stable Serine Protease From a Novel Thermo-Halotolerant Bacterial Strain, Laceyella sacchari Strain BK-TM.","authors":"Mohammed Amine Dahmani, Noureddine Boulenouar, Omar Alami, Boudjema Saoudi, Khelifa Bouacem, Sondes Mechri, Fawzi Allala, Omar Messaoudi, Mohamed Yousfi, Marilize Le Roes-Hill, Bassem Jaouadi","doi":"10.1002/bab.70015","DOIUrl":"https://doi.org/10.1002/bab.70015","url":null,"abstract":"<p><p>Laceyella sacchari strain BK-TM, isolated from the Salt Lake of Chott Ech Chergui (El-Bayadh, Algeria), was found to produce an extracellular thermostable serine protease (SPLS). The highest level of protease activity detected after 6 days of incubation at 50°C was 44,600 U/mL. SPLS was purified after heat treatment for 5 min at 80°C, subjected to ammonium sulfate fractionation (80%), and Sephacryl S-200 High Resolution (HR) column purification. The purified enzyme consists of a single protein with an approximate molecular weight of 29 kDa as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The sequence of the 24 N-terminal residues of SPLS was highly similar to those of Thermoactinomycetaceae proteases. Furthermore, the complete inhibition by phenylmethanesulfonyl fluoride (PMSF) and diiodopropyl fluorophosphates (DIFP) indicates that SPLS is a member of the serine protease family. The enzyme exhibits optimal activity at pH 9 and 70°C. The thermostability of SPLS increased when 1 mM of Ca²⁺ was added, with a half-life of 8 h at 80°C and 3 h at 90°C. Its catalytic activity was superior to that of SPSM from Streptomyces mutabilis strain TN-X30, PREFERENZ P300, and PROTEASE Type XIV. Interestingly, SPLS demonstrated high compatibility with ISIS and Maison Det, serving as solid and liquid laundry detergents, respectively. Furthermore, performance evaluations revealed that SPLS effectively removes blood stains. Overall, SPLS exhibited interesting biochemical features, suggesting its potential use as a cleaning bio-additive in detergent formulations.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144474047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Valorization of Biodiesel Waste, Glycerine Pitch as a Substrate for Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) Biosynthesis.","authors":"Rozina Kakar, Siti Nor Syairah Anis, Sevakumaran Vigneswari, Amirul Al-Ashraf Abdullah","doi":"10.1002/bab.70011","DOIUrl":"https://doi.org/10.1002/bab.70011","url":null,"abstract":"<p><p>Studies that focus on turning biodiesel byproducts into valuable products have recently garnered increasing attention. This investigation highlights the utilization of waste substrates for a circular economy approach using glycerine pitch as the main carbon source to produce biodegradable poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [(P(3HB-co-3HV)] by Cupriavidus malaysiensis USMAA1020. The primary use of glycerine pitch, along with several parameters that may affect the growth and biosynthesis of copolymers, are investigated. The utilization of glycerine pitch along with 1-pentanol and oleic acid has the most effective effect on bacterial growth and copolymer accumulation. Response surface methodology (RSM) was used to optimize these variables. The polyhydroxyalkanoate (PHA) content increased up to 77.7 wt% from 68.9 wt%, with residual dry cell weight (RDCW) of 6.5 g/L from 4.5 g/L. The optimal conditions for producing various compositions of the 3HVs were also determined. The three selected copolymer compositions were P(3HB-co-4%3HV), P(3HB-co-11%3HV), and P(3HB-co-18%3HV). Moreover, varying the copolymer compositions produced distinct polymer characteristics. According to this study, P(3HB-co-3HV) with variable properties can be produced for a range of applications using glycerine pitch as a potential primary carbon source. In addition to reducing the cost of production, this would enhance efficient waste management.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144474048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Orexin A Ameliorates Ovarian Dysfunction in Premature Ovarian Insufficiency Mice by Inhibiting Granulosa Cell Pyroptosis via the AMPK/NLRP3 Pathway.","authors":"Fengqin Yu, Tengzhen Wu, Lina Li, Bin Yang, Meixia Yin","doi":"10.1002/bab.2791","DOIUrl":"https://doi.org/10.1002/bab.2791","url":null,"abstract":"<p><p>Premature ovarian insufficiency (POI) is a condition characterized by the early depletion of ovarian follicles, leading to infertility and various systemic complications. Granulosa cell (GC) pyroptosis contributes significantly to the pathogenesis of POI. Orexin A, a neuropeptide involved in regulating wakefulness, has been shown to exert anti-inflammatory effects. This study investigates the potential protective role of orexin A in POI by targeting pyroptosis in ovarian GCs. We found that orexin A significantly reduced oxidative stress and the activation of the NLRP3 inflammasome in POI mice, thereby improving serum hormone levels and follicle count. Additionally, orexin A inhibited pyroptosis in cyclophosphamide (CTX)-treated KGN cells by downregulating NLRP3, caspase-1, and gasdermin D (GSDMD) expression. These effects were mediated through the activation of adenosine 5'-monophosphate-activated protein kinase (AMPK) signaling, which is known to regulate cellular metabolism and suppress inflammasome activation. In conclusion, orexin A has the potential to alleviate POI by inhibiting NOD-like receptor thermal protein domain-associated protein 3 (NLRP3) inflammasome-mediated pyroptosis and activating AMPK signaling, offering a promising therapeutic approach for POI treatment.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Probiotic and Synbiotic Isolates From Whey Water and Fermented Rice Water Exert Compelling Wound-Healing Efficacy in a Diabetic Mouse Model.","authors":"Sriya Choudhury, Ishita Saha, Bidyut Bandyopadhyay, Priyajit Banerjee","doi":"10.1002/bab.70013","DOIUrl":"https://doi.org/10.1002/bab.70013","url":null,"abstract":"<p><p>Probiotics serve as essential wound healers, particularly in diabetic patients, where they eliminate the interfering hindrance caused due to enhanced oxidative stress, inflammation, and microbial imbalances. Probiotic strains are also reported to enhance the level of tissue regeneration-specific cytokines and growth factors. Similarly, probiotics also stop infections by preventing the growth of harmful bacteria at the lesion site, which may be helpful for susceptible diabetic ulcers. Thus, probiotics may be a viable adjuvant treatment to hasten the healing of diabetic wounds. The present study aims to evaluate the efficacy of probiotic and synbiotic supplementation on wound healing in diabetic mice. Accordingly, probiotic strains Bacillus cereus strain BWN SC and Bacillus aerophilus strain BWN SC were isolated from whey water and fermented rice water on MRS agar media. Their wound-healing potential was investigated in male Swiss albino mice. Initially, streptozotocin was used to induce diabetes in the animals, after which 3 mm subcutaneous puncture wounds were induced on the dorsal region. Next, they were treated with different probiotics or synbiotics applied topically on the wounded area. Simultaneously, oral supplementation of the respective strains was also done using gavage. Wound contraction rate was studied at intervals of 1, 5, 10, and 14 days, followed by subsequent histological assay and hematological analysis. Animals treated with probiotics and synbiotics supplementation showed a faster wound contraction rate as compared to the control. Hematological analysis also showed improved wound-healing parameters in the treated mice. Thus, isolated probiotic strains can be effectively used for wound healing in diabetic mice. They can be combined with prebiotics to get even more potent therapeutic tool in the form of synbiotics.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antidiabetic Potential of Synthesized Ferrocenylmethylaniline Derivatives: Insights From In Vitro Studies, Molecular Docking, ADMET, DFT Calculations, and Molecular Dynamics Simulation.","authors":"Yahia Bekkar, Elhafnaoui Lanez, Touhami Lanez, Lotfi Bourougaa, Aicha Adaika, Zahra Saada, Aida Benine","doi":"10.1002/bab.70014","DOIUrl":"https://doi.org/10.1002/bab.70014","url":null,"abstract":"<p><p>This study investigates the potential of three synthesized ferrocenylmethylaniline derivatives (FMBA, FMAA, and FMA) as inhibitors of α-amylase, a key enzyme involved in the pathophysiology of diabetes. In vitro inhibition assays demonstrated that FMBA and FMAA exhibited significantly lower IC50 values of 9.23 and 11.23 µM, respectively, compared to 259 µM for the standard drug acarbose (ARE). Molecular docking studies supported these findings, with FMBA showing the highest binding affinity (∆G of -7.33 kcal/mol), followed by FMAA (-6.44 kcal/mol) and FMA (-5.85 kcal/mol), outperforming ARE (-4.88 kcal/mol). ADMET analysis suggested favorable pharmacokinetic and safety profiles for FMBA and FMAA, reinforcing their potential as viable drug candidates. To further assess the stability and dynamics of the enzyme-ligand interactions, molecular dynamics simulations were conducted, showing that FMBA and FMAA formed significantly more stable complexes with α-amylase compared to ARE, as indicated by low root mean square deviation (RMSD) values of 0.156 and 0.164 nm, respectively, compared to 0.359 nm for ARE. Root mean square fluctuation (RMSF) analysis revealed consistent stability at key active site residues. Additional analyses of radius of gyration (R_g) and solvent-accessible surface area (SASA) supported the compact and stable nature of the complexes. Frontier molecular orbital (FMO) analysis showed smaller HOMO-LUMO energy gaps for FMBA and FMAA, suggesting greater reactivity and potential biological activity. Molecular electrostatic potential (MEP) surface analysis highlighted key reactive sites, with high negative potential localized on the carbonyl groups of FMBA and FMAA, and high positive potential in regions favoring hydrogen bonding. These findings underscore the potential of FMBA and FMAA as promising antidiabetic agents and support their further development as therapeutic candidates.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Impact of Hunched Posture on the Elasticity of the Upper and Middle Trapezius Muscles During Sedentary Activity.","authors":"Rama Krishna Reddy Guduru, S S Saravana Kumar, M M Ramakrishna, Joshna Ramineni, Bennila Thangammal Chandrasekaran, Shiva Kumar Badiganchela, S R Mugunthan","doi":"10.1002/bab.70008","DOIUrl":"https://doi.org/10.1002/bab.70008","url":null,"abstract":"<p><p>Prolonged sitting is often associated with poor posture, hunched shoulder posture (HSP), which may alter the mechanical properties of key muscles such as the upper trapezius (UT) and middle trapezius (MT). This study aimed to investigate the effect of HSP on the elasticity of the UT and MT muscles during a sitting task and to gain insight into the musculoskeletal risks associated with postural errors while sitting. Thirty-four asymptomatic individuals participated in this study between the ages of 25 and 40, who led sedentary lifestyles with 7 h of sedentary activity per day. None of the participants had a history of neurological, systemic, or musculoskeletal diseases that may affect muscle function. The MyotonPRO device was used to measure UT and MT muscle elasticity during normal shoulder posture (NSP) and HSP. Statistical analyses were performed to compare the mechanical properties of the UT and MT muscles across the different postural groups. The findings revealed that the mean elasticity of the UT increased from 1.25 (±0.30) under NSP to 1.30 (±0.32) under HSP. The MT exhibited a more pronounced change, with mean elasticity rising from 1.73 (±0.30) under NSP to 2.12 (±0.34) under HSP. These results indicate significant adaptations in muscle elasticity due to poor postural alignment, suggesting a compensatory response to the mechanical demands of HSP (p < 0.001). The study highlights the detrimental effects of hunched posture on the mechanical properties of the upper and MT muscles, emphasizing the need for ergonomic interventions to promote better postural alignment among sedentary workers. Regular breaks and exercises aimed at improving posture are recommended to mitigate the risks associated with prolonged poor posture, ultimately enhancing worker well-being and productivity.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144309552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association Between Different Types of Physical Activity and the Risk of COPD: A Cross-Sectional NHANES Study.","authors":"Lindong Yuan, Juan Zheng, Zhe Chen","doi":"10.1002/bab.70004","DOIUrl":"https://doi.org/10.1002/bab.70004","url":null,"abstract":"<p><p>Chronic obstructive pulmonary disease (COPD) is a progressive condition that significantly impairs respiratory function. While known risk factors such as smoking and air pollution are well established, the role of physical activity in the development of COPD remains unclear. This study aimed to explore the association between different types of physical activity and COPD risk using data from 14,579 adults aged 40 years and older who participated in the National Health and Nutrition Examination Survey (NHANES) from 2007 to 2018, representing a weighted population of approximately 93.9 million. A weighted binary logistic regression model was employed to evaluate the relationship between various forms of physical activity-leisure, occupational, and transportation-related-and COPD risk, adjusting for multiple covariates including age, sex, race, BMI, smoking status, sedentary time, and comorbidities. COPD was defined based on self-reported physician diagnosis and spirometry criteria. Among the participants, 8.6% had COPD. Results showed that individuals who engaged in moderate-intensity leisure physical activity had a significantly lower risk of COPD (OR: 0.75; 95% CI: 0.61.0.91; P = 0.005). No significant associations were found for high-intensity leisure activity, work-related physical activity, or walking/cycling as a mode of transportation. Subgroup analyses indicated that the protective effect of moderate-intensity leisure activity varied across demographic and behavioral subgroups. These findings suggest that only moderate-intensity leisure physical activity is associated with reduced COPD risk, highlighting the potential preventive role of this specific type of exercise.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144309550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and Functional Characterization of a Lipopeptide Biosurfactant From a Novel Novosphingobium lentum TR17 Strain With Potential for Industrial and Bioremediation Applications.","authors":"Atipan Saimmai, Suppasil Maneerat, Wiboon Riansa-Ngawong, Kattinat Sagulsawasdipan, Chanika Saenge Chooklin","doi":"10.1002/bab.2788","DOIUrl":"https://doi.org/10.1002/bab.2788","url":null,"abstract":"<p><p>Biosurfactant-producing bacteria were isolated from used lubricating oil (ULO)-contaminated soil in Phuket and Trang, southern Thailand. Screening for biosurfactant production was conducted using surface tension reduction and emulsification activity. The highest surface tension reduction was observed in a strain identified as Novosphingobium lentum TR17 using 16S rRNA gene analysis. The selected strain demonstrated optimal biosurfactant production using glucose and commercial monosodium glutamate as carbon and nitrogen sources, respectively. After 60 h of cultivation, the biosurfactant yield reached 5.30 g/L, with a surface tension reduction from 72 to 25 mN/m. The biosurfactant, recovered using a chloroform:methanol solvent system, exhibited thermal and pH stability, a low critical micelle concentration (9 mg/L), and high emulsification activity. Fourier-transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR), and mass spectrometry confirmed its lipopeptide nature. Additionally, the crude biosurfactant demonstrated antibacterial properties and enhanced the solubility of polyaromatic hydrocarbons (PAHs), suggesting its potential for bioremediation and industrial applications.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":"e2788"},"PeriodicalIF":3.2,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144309551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis, Characterization, Biological Properties, and Sensor Applications of Novel Thiosemicarbazone Derivatives.","authors":"Hayreddin Gezegen, Oguz Özbek, Meryem Topal, Hatice Şahin, Ümit M Koçyiğit","doi":"10.1002/bab.70001","DOIUrl":"https://doi.org/10.1002/bab.70001","url":null,"abstract":"<p><p>In this study, novel thiosemicarbazone derivative molecules (5a-j) were synthesized. The characterization of the synthesized molecules was carried out using ¹H-, ¹³C-NMR, FT-IR, and Q-TOF spectroscopic techniques. Then, the inhibitory effect of these compounds was examined for human carbonic anhydrase I and II (hCA I, and II) and acetylcholinesterase (AChE). The hCA I II, and AChE were efficiently inhibited by synthesized thiosemicarbazone derivatives, with Ki values in the range of 0.293-1.666 µM for hCA I, 0.298-1.332 µM for hCA II, and 52.51-138.63 µM for AChE, respectively. Finally, the sensor properties of the synthesized thiosemicarbazone derivatives were investigated. The newly synthesized thiosemicarbazone derivative molecules exhibited high selectivity towards Pb²⁺ ions. The sensor prepared with 5a from these molecules had a Nernstian behavior of 28.8 ± 1.5 mV/decade and a low detection limit of 2.86 × 10^-6 M in a wide concentration range of 1.0 × 10^-1-1.0 × 10^-5 M. The newly proposed sensor also had a wide pH working range (5.0-10.0), a very fast response time (5 s), and good reproducibility. The newly fabricated sensors were used as indicator electrodes for the potentiometric titration of Pb²⁺ ions with ethylenediaminetetraacetic acid (EDTA) and were successfully applied for the determination of Pb²⁺ ion content in different water samples.</p>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144246571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}