Solubilizer of bacterial origin surfactin increases the biological activity of C60 fullerene

IF 3.2 4区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biotechnology and applied biochemistry Pub Date : 2024-09-11 DOI:10.1002/bab.2665

Sergey Emelyantsev, Evgeniya Prazdnova, Vladimir Chistyakov

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引用次数: 0

Abstract

Currently, there exists conflicting data regarding the biological activity of unmodified fullerene C60. Various sources report its toxicity, geroprotective activity, and potential interaction with DNA. Contradictory findings regarding the toxicity of C60 may arise from the use of toxic solvents, as well as the influence of bioavailability and bioactivity on the preparation conditions of C60 suspensions. Furthermore, the microbiota of experimental animals can impact geroprotective activity results by releasing surfactants that facilitate substance penetration through the cell membrane. In this study, we selected conditions for solubilizing fullerene C60 in a solution of surfactin, a surfactant of bacterial origin, as well as in a 2% aqueous solution of TWEEN 80, employing ultrasound. Through bioluminescent analysis using lux biosensors in Escherichia coli MG1655, we observed that C60 in surfactin reduced induced genotoxic and oxidative stress. Given that surfactin enhances membrane permeability to fullerene C60, suspensions of fullerene in designated concentrations of surfactin can be regarded as a DNA protector and antioxidant, warranting further investigation as a promising component of novel drugs.

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细菌源表面活性素增溶剂提高了 C60 富勒烯的生物活性

目前，有关未改性富勒烯 C60 生物活性的数据相互矛盾。各种来源都报道了它的毒性、老年保护活性以及与 DNA 的潜在相互作用。有关 C60 毒性的矛盾研究结果可能是由于使用了有毒溶剂，以及 C60 悬浮液的制备条件对生物利用率和生物活性的影响。此外，实验动物的微生物群会释放表面活性剂，促进物质穿透细胞膜，从而影响老年保护活性的结果。在这项研究中，我们利用超声波选择了富勒烯 C60 在表面活性素（一种源于细菌的表面活性剂）溶液和 2% TWEEN 80 水溶液中的增溶条件。通过在大肠杆菌 MG1655 中使用勒克斯生物传感器进行生物发光分析，我们观察到表面活性剂中的 C60 降低了诱导的基因毒性和氧化应激。鉴于表面活性剂能增强膜对富勒烯 C60 的渗透性，富勒烯在指定浓度的表面活性剂中的悬浮液可被视为一种 DNA 保护剂和抗氧化剂，值得进一步研究，有望成为新型药物的一种成分。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biotechnology and applied biochemistry 工程技术-生化与分子生物学

CiteScore

6.00

自引率

7.10%

发文量

117

审稿时长

3 months

期刊介绍： Published since 1979, Biotechnology and Applied Biochemistry is dedicated to the rapid publication of high quality, significant research at the interface between life sciences and their technological exploitation. The Editors will consider papers for publication based on their novelty and impact as well as their contribution to the advancement of medical biotechnology and industrial biotechnology, covering cutting-edge research in synthetic biology, systems biology, metabolic engineering, bioengineering, biomaterials, biosensing, and nano-biotechnology.