基于纳米银瞬效唑杂化乳液的益生菌是一种新型抗真菌配方，可用于抗耐多药的阴道病原体

IF 3.2 4区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biotechnology and applied biochemistry Pub Date : 2024-09-15 DOI:10.1002/bab.2663

Farag M. Mosallam, Eman A. Helmy, Hanan S. El-Bastawisy, Ahmed I. El-Batal

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引用次数: 0

摘要

本研究提出了一种治疗念珠菌性阴道炎的新方法，即利用新型瞬效唑纳米银杂化乳剂（Ag-Secn-NHE）为基础的益生菌和游离Ag-Secn-NHE。Ag-Secn-NHE 采用简单的均质-超声技术制备，并通过紫外-可见光扫描、动态光散射、透射电子显微镜（TEM）、傅立叶变换红外光谱和 zeta 电位进行了验证。酿酒酵母（RCMB 002Y001）是最有效的益生菌，与 Ag-Secn-NHE 结合使用时效果显著。与游离的 Ag-Secn-NHE、硝酸银、纳米银颗粒、昔尼达唑、昔尼达唑纳米乳剂和商用阴道洗液相比，基于 Ag-Secn-NHE 的益生菌对耐多药阴道病原体具有显著的抗真菌效果。基于 Ag-Secn-NHE 的益生菌对白色念珠菌、白色念珠菌和新生隐球菌的抑制效果最高，最小抑菌浓度（MIC）分别为 0.625 ± 0.002、0.00625 ± 0.002、0.00625 ± 0.002。002、0.00625:1.25 ± 0.012 和 0.00625:1.25 ± 0.032 mg/mL，而 Ag-Secn-NHE 的 MIC 为 0.00625:1.25 ± 0.612、0.0125:2.5 ± 0.812 和 0.0125:2.5 ± 0.112 mg/mL（Ag:Secn）。基于 Ag-Secn-NHE 的益生菌的最低杀菌浓度为 2.5 至 20 毫克/毫升，而游离 Ag-Secn-NHE 的最低杀菌浓度为 5 至 20 毫克/毫升。此外，与游离Ag-Secn-NHE相比，基于Ag-Secn-NHE的益生菌能抑制75%的阴沟球菌生物膜形成，抑制60%的新生隐球菌和白僵菌生物膜形成。时间杀伤曲线显示，基于 Ag-Secn-NHE 的益生菌的抗真菌效果在 4 小时后达到 2MIC 值，16 小时后达到 Ag-Secn-NHE 的 2MIC 值。TEM 照片显示，用 Ag-Secn-NHE 型益生菌配方处理的 C. auris 细胞出现了严重的变形和超微结构变化。

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Silver secnidazole nano-hybrid emulsion-based probiotics as a novel antifungal formula against multidrug-resistant vaginal pathogens

This study presents a novel approach to manage vaginal infections due to Candidiasis, utilizing a novel silver secnidazole nano-hybrid emulsion (Ag-Secn-NHE)-based probiotics and free Ag-Secn-NHE. Ag-Secn-NHE was prepared by simple homogenization‒ultrasonication technique and validated by using a ultraviolet‒visible scan, dynamic light scattering, transmission electron microscopy (TEM), Fourier-transform infrared spectroscopy, and zeta potential. Saccharomyces cerevisiae (RCMB 002Y001) is the most effective probiotic-producing organism that demonstrates significant effects when combined with Ag-Secn-NHE. Ag-Secn-NHE-based probiotics showed significant antifungal effect compared to free Ag-Secn-NHE, silver nitrate, silver nanoparticles, secnidazole, secnidazole nanoemulsion, and commercial vaginal wash against multidrug-resistant vaginal pathogens. The highest inhibitory effect was achieved with Ag-Secn-NHE-based probiotic against Candida auris, Candida albicans, and Cryptococcus neoformans with minimal inhibitory concentration (MIC) 0.625 ± 0.002, 0.00625:1.25 ± 0.012 and 0.00625:1.25 ± 0.032 mg/mL, respectively, in comparison with Ag-Secn-NHE that show MIC at 0.00625:1.25 ± 0.612, 0.0125:2.5 ± 0.812, and 0.0125:2.5 ± 0.112 mg/mL (Ag:Secn). Ag-Secn-NHE-based- probiotic show minimum fungicidal concentration (MFC) at range from 2.5 to 20 mg/mL, wherever free Ag-Secn-NHE show MFC range from 5 to >20 mg/mL. Additionally, Ag-Secn-NHE-based probiotics have 75% inhibition of biofilm formation against C. auris and 60% inhibition of biofilm formation against both Cryptococcus neoformans and C. albicans in comparison with free Ag-Secn-NHE. Time-kill curves showed that the antifungal effect of Ag-Secn-NHE-based probiotics was fungistatic at 2MIC value after 4 h and after 16 h for Ag-Secn-NHE. TEM photographs showed that C. auris cells treated with Ag-Secn-NHE-based probiotic formula revealed severe deformations and distored ultrastructural changes. furthermore, results indicated that the Gamma radiation up to 15 kGy increases production of Ag-Secn-NHE in comparison with non-irradiated one.

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来源期刊

Biotechnology and applied biochemistry 工程技术-生化与分子生物学

CiteScore

6.00

自引率

7.10%

发文量

117

审稿时长

3 months

期刊介绍： Published since 1979, Biotechnology and Applied Biochemistry is dedicated to the rapid publication of high quality, significant research at the interface between life sciences and their technological exploitation. The Editors will consider papers for publication based on their novelty and impact as well as their contribution to the advancement of medical biotechnology and industrial biotechnology, covering cutting-edge research in synthetic biology, systems biology, metabolic engineering, bioengineering, biomaterials, biosensing, and nano-biotechnology.