Biotechnology and applied biochemistry最新文献_第8页

Protective efficacy of nafronyl in diabetic retinopathy through targeted inhibition of key enzymes. 通过靶向抑制关键酶，萘呋胺对糖尿病视网膜病变具有保护作用。

IF 3.2 4区生物学

Biotechnology and applied biochemistry Pub Date : 2024-06-19 DOI: 10.1002/bab.2625

Eyush Eyush, Shivani Kumar, Karishma Sen, Anita Sakarwal, Heera Ram, Dharamveer Yadav, Antresh Kumar, Anil Panwar

{"title":"Protective efficacy of nafronyl in diabetic retinopathy through targeted inhibition of key enzymes.","authors":"Eyush Eyush, Shivani Kumar, Karishma Sen, Anita Sakarwal, Heera Ram, Dharamveer Yadav, Antresh Kumar, Anil Panwar","doi":"10.1002/bab.2625","DOIUrl":"10.1002/bab.2625","url":null,"abstract":"Diabetic retinopathy is governed by abnormal apoptosis, increased capillary pressure, and other linked pathology that needs an efficient treatment by multitargeted approaches. Thus, the current study aimed to explore the potential of inhibition of targeted enzymes (DPP4, ACE-2, and aldose reductase) and free radical scavenging capabilities of selected compounds (nafronyl or naftidrofuryl) through in silico and in vivo investigations. Significant binding energies were observed in complexes of aldolase reductase, angiotensin type 1 receptor, and DPP4 against the nafronyl and sitagliptin more than -7.5 kcal/mol. Further validation of free energy was confirmed by calculations of molecular mechanics Poisson-Boltzmann surface area (MMPBSA), and configurational stabilities examined by PCA (principal component analysis). Additionally, drug-likeness was examined by the Swiss ADME web tool, which showed significant findings. Consequently, in vivo experimentations showed significant inflammation and alterations in retinal layers of inner plexiform (inner limiting membrane, nerve fibers, and ganglionic cells), inner nuclear layer (bipolar cells and horizontal cells), and photoreceptors cells. Whereas the treatments (nafronyl and sitagliptin) caused significant improvements in the histoarchitecture of the retina. Additionally, the HOMA indices (IR-insulin resistance, sensitivity, and β cells functioning) and levels of free radicals were significantly altered in the diabetic control group in comparison to intact control. Nafronyl administration showed significant ameliorations in HOMA indices as well as antioxidant levels. Based on the results, it can be concluded that nafronyl efficiently interacts with target enzymes, which may result in potent inhibition and ameliorations in retinal histology as well as glucose homeostasis and antioxidants.","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141426311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gold nanourchin on multiple-point dielectrode for glucose biosensing by current-potential measurement. 通过电流电位测量法在多点电极上进行葡萄糖生物传感的纳米金。

IF 2.8 4区生物学

Biotechnology and applied biochemistry Pub Date : 2024-06-12 DOI: 10.1002/bab.2626

Wei Chen, Lili Huang, Bing Zhou

{"title":"Gold nanourchin on multiple-point dielectrode for glucose biosensing by current-potential measurement.","authors":"Wei Chen, Lili Huang, Bing Zhou","doi":"10.1002/bab.2626","DOIUrl":"https://doi.org/10.1002/bab.2626","url":null,"abstract":"Gestational diabetes (GD) is a condition characterized by elevated blood sugar levels during pregnancy. GD poses various health risks, such as serious birth injuries, the need for cesarean delivery, and the necessity of newborn care. Monitoring glucose levels is essential for ensuring safe delivery and reducing the risks to both the mother and fetus. Various sensors are readily available for monitoring glucose levels, and researchers are continually working to develop highly sensitive glucose sensors. This research aimed to develop a gold nanourchin (AuNU)-hybrid biosensor for quantifying glucose on a multi-point electrode sensor. Glucose oxidase (GOx) was attached to the AuNU and seeded on the sensing surface using an amine linker. The current-potential (1-2 V at 0.1 V sweep) was recorded for the GOx-glucose interaction, with a limit of detection of 560 μM and a regression coefficient (R2) of 0.9743 [y = 0.9106x - 0.9953] on the linear curve. The sensitivity was estimated to be 3.5 mAcm-2M-1. Furthermore, control experiments with galactose, sucrose, and fructose did not yield an increase in current-potential, confirming specific glucose detection. This experiment helps in monitoring glucose levels to manage conditions associated with GD.","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141310087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Catalytically active inclusion bodies as a potential tool for biotechnology. 作为生物技术潜在工具的催化活性包涵体。

IF 2.8 4区生物学

Biotechnology and applied biochemistry Pub Date : 2024-06-11 DOI: 10.1002/bab.2624

Muhammad Nura Bello, Suriana Sabri, Normi Mohd Yahaya, Fairolniza Mohd Shariff, Mohd Shukuri Mohamad Ali

{"title":"Catalytically active inclusion bodies as a potential tool for biotechnology.","authors":"Muhammad Nura Bello, Suriana Sabri, Normi Mohd Yahaya, Fairolniza Mohd Shariff, Mohd Shukuri Mohamad Ali","doi":"10.1002/bab.2624","DOIUrl":"https://doi.org/10.1002/bab.2624","url":null,"abstract":"The initial assumption that viewed inclusion bodies as a hindrance to the efficient production of protein is no longer held due to the emergence of catalytically active inclusion bodies (CatIBs). Recent studies revealed their potential to be used in free form or immobilized as biocatalysts. The curiosity to acquire suitable catalysts has remained the measure of concern for researchers and industrialists. Numerous processes and production in various sectors of food industries, petroleum, pharmaceutical, cosmetics, and many others are still searching for a robust catalyst with outstanding features such as recyclability, resistance to pH, as well as temperature. CatIBs are forms of inclusion bodies that possess catalytic activity, which can improve catalysis efficiency, stability, and recyclability. One of the advantages of CatIBs is their potential to be used as catalysts for numerous bioprocesses when generated by an enzyme. These aggregates can efficiently be used as a replacement for traditional enzyme immobilization. This review tends to focus on the possibility of its application in various processes. The novelty of this review is that it considered the production of CatIBs both from artificial and natural perspectives, as well as how to improve it. Inclusion bodies' immobilization may provide an efficient alternative in the area of biocatalysis, and hence it will improve industrial sectors and substantially provide a means of achieving excellent performance in the near future.","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141305494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pelargonidin inhibits cell growth and promotes oxidative stress-mediated apoptosis in lung cancer A549 cells 天竺葵苷能抑制肺癌 A549 细胞的生长，并促进氧化应激介导的细胞凋亡。

IF 3.2 4区生物学

Biotechnology and applied biochemistry Pub Date : 2024-06-09 DOI: 10.1002/bab.2621

Liwei Yue, Ying Li, Yuting Luo, Abdullah A. Alarfaj, Yubo Shi

{"title":"Pelargonidin inhibits cell growth and promotes oxidative stress-mediated apoptosis in lung cancer A549 cells","authors":"Liwei Yue, Ying Li, Yuting Luo, Abdullah A. Alarfaj, Yubo Shi","doi":"10.1002/bab.2621","DOIUrl":"10.1002/bab.2621","url":null,"abstract":"Lung cancer has the worst prognosis with an average 5-year survival rate of only 10%–20%. Lung cancer has the highest prevalence rate and a second most common cause of cancer-associated mortalities worldwide. The present study was planned to explore the anticancer effects of pelargonidin against the lung cancer A549 cells via analyzing oxidative stress-mediated apoptosis. The viability of both control and pelargonidin-treated A549 cells was analyzed using the MTT cytotoxicity assay at different time periods. The levels of endogenous ROS generation, mitochondrial membrane potential (Δψm), and apoptosis were assessed using corresponding fluorescent staining assays. The levels of oxidative stress biomarkers, including TBARS, SOD, CAT, and GSH, in the cell lysates of control and pelargonidin-treated A549 cells were examined using the assay kits. The pelargonidin treatment substantially suppressed the A549 cell growth. Further, pelargonidin promoted the ROS production and depleted the Δψm levels in the A549 cells. The fluorescent staining assays witnessed the occurrence of increased apoptosis in the pelargonidin-treated A549 cells. The pelargonidin also boosted the TBARS and reduced the antioxidant levels thereby promoted the oxidative stress-regulated apoptosis in the A549 cells. In summary, the findings’ results of the current study demonstrated an anticancer activity of pelargonidin on A549 cells. The pelargonidin treatment substantially decreased the growth and encouraged the oxidative stress-regulated apoptosis in A549 cells. Therefore, it was evident that the pelargonidin could be employed as an effective anticancer candidate to treat the lung cancer.","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":"71 5","pages":"1195-1203"},"PeriodicalIF":3.2,"publicationDate":"2024-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141295583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening, characterization, and optimization of the fermentation conditions of a novel cellulase-producing microorganism from soil of Qinghai-Tibet Plateau. 青藏高原土壤中一种新型纤维素酶生产微生物的筛选、表征及发酵条件优化

IF 2.8 4区生物学

Biotechnology and applied biochemistry Pub Date : 2024-06-06 DOI: 10.1002/bab.2622

Longmei Zhao, Lan Zhang, Yaning Qin, Wang Li, Yuanxiao Li, Hui Cao, Pinghua Cao, Ke Ding, Wanling He

{"title":"Screening, characterization, and optimization of the fermentation conditions of a novel cellulase-producing microorganism from soil of Qinghai-Tibet Plateau.","authors":"Longmei Zhao, Lan Zhang, Yaning Qin, Wang Li, Yuanxiao Li, Hui Cao, Pinghua Cao, Ke Ding, Wanling He","doi":"10.1002/bab.2622","DOIUrl":"https://doi.org/10.1002/bab.2622","url":null,"abstract":"Cellulases play an important role in the bioconversion of lignocellulose. Microorganisms found in extreme environments are a potentially rich source of cellulases with unique properties. Due to the uniqueness of the environment, the abundant microbial resources in the Qinghai-Tibet Plateau (QTP) are worth being explored. The aim of this study was to isolate and characterize an acidic, mesophilic cellulase-producing microorganism from QTP. Moreover, the fermentation conditions for cellulase production were optimized for future application of cellulase in the development of lignocellulose biomass. A novel cellulase-producing strain, Penicillium oxalicum XC10, was isolated from the soil of QTP. The cellulase produced by XC10 was a mesophilic cellulase that exhibited good acid resistance and some cold-adaptation properties, with maximum activity at pH 4.0 and 40°C. Cellulase activity was significantly enhanced by Na+ (p < 0.05) and inhibited by Mg2+, Ca2+, Cu2+, and Fe3+ (p < 0.05). After optimization, maximum cellulase activity (8.56 U/mL) was increased nearly 10-fold. Optimal fermentation conditions included an inoculum size of 3% (v/v) in a mixture of corn straw (40 g/L), peptone (5 g/L), and Mg2+ (4 g/L), at pH 4.0, 33°C, and shaking at 200 rpm. The specific properties of the P. oxalicum XC10 cellulase suggest the enzyme may serve as an excellent candidate for the bioconversion and utilization of lignocellulose biomass generated as agricultural and food-processing wastes.","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141283051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ohwia caudata inhibits doxorubicin-induced cardiotoxicity by regulating mitochondrial dynamics via the IGF-IIR/p-Drp1/PARP signaling pathway Ohwia caudata 通过 IGF-IIR/p-Drp1/PARP 信号通路调节线粒体动力学，从而抑制多柔比星诱导的心脏毒性

IF 3.2 4区生物学

Biotechnology and applied biochemistry Pub Date : 2024-06-04 DOI: 10.1002/bab.2620

Jhong-Kuei Chen, Samiraj Ramesh, Md. Nazmul Islam, Marthandam Asokan Shibu, Chia-Hua Kuo, Dennis Jine-Yuan Hsieh, Shinn-Zong Lin, Wei-Wen Kuo, Chih-Yang Huang, Tsung-Jung Ho

{"title":"Ohwia caudata inhibits doxorubicin-induced cardiotoxicity by regulating mitochondrial dynamics via the IGF-IIR/p-Drp1/PARP signaling pathway","authors":"Jhong-Kuei Chen, Samiraj Ramesh, Md. Nazmul Islam, Marthandam Asokan Shibu, Chia-Hua Kuo, Dennis Jine-Yuan Hsieh, Shinn-Zong Lin, Wei-Wen Kuo, Chih-Yang Huang, Tsung-Jung Ho","doi":"10.1002/bab.2620","DOIUrl":"10.1002/bab.2620","url":null,"abstract":"The most effective drug, doxorubicin (DOX), is widely used worldwide for clinical application as an anticancer drug. DOX-induced cytotoxicity is characterized by mitochondrial dysfunction. There is no alternative treatment against DOX-induced cardiac damage despite intensive research in the present decades. Ohwia caudata has emerged as a potential herbal remedy that prevents from DOX-induced cytotoxicity owing to its pharmacological action of sustaining mitochondrial dynamics by attenuating oxidative stress and inducing cellular longevity. However, its underlying mechanisms are unknown. The novel treatment provided here depends on new evidence from DOX-treated H9c2 cells, which significantly enhanced insulin-like growth factor (IGF) II receptor (IGF-IIR) pathways that activated calcineurin and phosphorylated dynamin-related protein 1 (p-Drp1) at ser616 (p-Drp1[ser616]); cells undergo apoptosis due to these factors, which translocate to mitochondria and disrupt their function and integrity, and in terms of herbal medicine treatment, which significantly blocked these phenomena. Thus, our findings indicate that maintaining integrity of mitochondria is an essential element in lowering DOX-induced cytotoxicity, which further emphasizes that our herbal medicine can successfully block IGF-IIR pathways and could potentially act as an alternative mechanism in terms of cardioprotective against doxorubicin.","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":"71 5","pages":"1181-1194"},"PeriodicalIF":3.2,"publicationDate":"2024-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141258438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Abnormal expression of serum miR-4746-5p in liver cancer patients after interventional chemotherapy and its possible mechanism 介入化疗后肝癌患者血清 miR-4746-5p 的异常表达及其可能机制

IF 3.2 4区生物学

Biotechnology and applied biochemistry Pub Date : 2024-05-29 DOI: 10.1002/bab.2605

Keping Deng, Wei Wang, Xiaobin Chi, Yan Yu, Yichuan Zhang, Jianming Yuan

{"title":"Abnormal expression of serum miR-4746-5p in liver cancer patients after interventional chemotherapy and its possible mechanism","authors":"Keping Deng, Wei Wang, Xiaobin Chi, Yan Yu, Yichuan Zhang, Jianming Yuan","doi":"10.1002/bab.2605","DOIUrl":"10.1002/bab.2605","url":null,"abstract":"Interventional chemotherapy is a common operation in the clinical treatment of liver cancer. The aim of this study was to investigate the expression and molecular mechanism of serum miR-4746-5p in liver cancer patients before and after interventional chemotherapy. The levels of miR-4746-5p and CDKN1C in serum samples from liver cancer patients were detected using real-time fluorescence quantitative polymerase chain reaction. Receiver operating characteristic curves revealed the diagnostic value of miR-4746-5p in tumors. Differences in clinical indicators between liver cancer patients and healthy controls were assessed using Pearson correlation analysis. Luciferase reporter gene assays confirmed the targeted interaction between miR-4746-5p and CDKN1C. In vitro cellular assays were validated by Cell Counting Kit-8, Transwell assay, and chemoresistance assay. Serum miR-4746-5p levels were increased in liver cancer patients but were downregulated after chemotherapy intervention. CDKN1C expression showed the opposite trend. Low levels of miR-4746-5p mediated cell growth and metastasis by targeting and negatively regulating CDKN1C expression, while silencing CDKN1C restored cell activity. Inhibition of miR-4746-5p reduced chemoresistance, while downregulation of CDKN1C affected cell sensitivity. miR-4746-5p may be a potential therapeutic factor for liver cancer diagnosis and interventional chemotherapy.","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":"71 5","pages":"1154-1163"},"PeriodicalIF":3.2,"publicationDate":"2024-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141174440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlation between the miR-618 rs2682818 C>A polymorphism and venous malformation susceptibility miR-618 rs2682818 C>A 多态性与静脉畸形易感性之间的相关性。

IF 3.2 4区生物学

Biotechnology and applied biochemistry Pub Date : 2024-05-28 DOI: 10.1002/bab.2618

Xi Lin, Zijian Chen, Guitao Wu, Hua Jiang, Zhenyin Liu

{"title":"Correlation between the miR-618 rs2682818 C>A polymorphism and venous malformation susceptibility","authors":"Xi Lin, Zijian Chen, Guitao Wu, Hua Jiang, Zhenyin Liu","doi":"10.1002/bab.2618","DOIUrl":"10.1002/bab.2618","url":null,"abstract":"Venous malformations are the most common congenital vascular malformations, and the incidence rate is high. Previous studies have confirmed that a variety of polymorphisms within the miRNA functional region are associated with tumor susceptibility. We examined the correlation between miR-618 rs2682818 C>A and risk of developing venous malformation in a southern Chinese population (1113 patients and 1158 controls). TaqMan genotyping of miR-618 rs2682818 C>A was conducted utilizing real-time fluorescent quantitative PCR. The miR-618 rs2682818 polymorphism was not correlated with susceptibility to venous malformation (CA/AA vs. CC: adjusted odds ratio [AOR] = 1.00, 95% confidence interval [CI] = 0.81–1.25, p = 0.994; AA vs. CC/CA: AOR = 1.10, 95% CI = 0.73–1.65, p = 0.646). Stratified analysis of different subtypes of venous malformation revealed that there was no significant difference in the rs2682818 C>A polymorphism genotypes across these subtypes. Our results indicate that miR-618 rs2682818 C>A polymorphism is not correlated with the susceptibility to venous malformation.","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":"71 5","pages":"1164-1169"},"PeriodicalIF":3.2,"publicationDate":"2024-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141157532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hyposalivation in patients with psoriasis: Association with severity, inflammatory, and anti-inflammatory cytokine biomarkers of the disease 银屑病患者唾液分泌过少：与疾病严重程度、炎症和抗炎细胞因子生物标志物的关系。

IF 3.2 4区生物学

Biotechnology and applied biochemistry Pub Date : 2024-05-28 DOI: 10.1002/bab.2619

Ravi Kant Sharma, Manu Rashmi Sharma, Aneet Mahendra, Simranjit Singh, Shaveta Sood, Sushil Kumar Upadhyay, Anil Kumar Sharma

{"title":"Hyposalivation in patients with psoriasis: Association with severity, inflammatory, and anti-inflammatory cytokine biomarkers of the disease","authors":"Ravi Kant Sharma, Manu Rashmi Sharma, Aneet Mahendra, Simranjit Singh, Shaveta Sood, Sushil Kumar Upadhyay, Anil Kumar Sharma","doi":"10.1002/bab.2619","DOIUrl":"10.1002/bab.2619","url":null,"abstract":"The aim of the study is to expound the effect of psoriasis on salivary glands by evaluating the secretion of saliva and salivary cytokine biomarkers in patients with psoriasis. This study was conducted by recruiting 120 subjects that included 60 patients diagnosed clinically with active psoriasis and 60 healthy controls who were age and gender matched to psoriatic subjects. Unstimulated whole saliva was collected from all the subjects by spitting method, and levels of tumor necrosis factor-alpha (TNF-α), interferon-gamma (IFN-γ), interleukin-2 (IL-2), and IL-10 (IL-10) were determined via enzyme-linked immunosorbent assay (BT Lab, Shanghai, China). Secretion of saliva in psoriasis patients was considerably reduced than in healthy controls. The concentrations of pro-inflammatory cytokines (TNF-α, IFN-γ, and IL-2) were significantly increased, whereas level of anti-inflammatory cytokine (IL-10) was markedly decreased in the saliva of psoriasis patients with hyposalivation compared to healthy subjects. Our results demonstrated significant negative correlation of salivary flow rates with the disease severity. No significant correlations were obtained between salivary levels of tested cytokines and salivary flow rates in our study. Findings of the study reflect inflammation of salivary glands with reduced salivary flow rates in psoriasis patients. The inflammatory responses in salivary gland tissues by virtue of increased pro-inflammatory cytokines concentrations together with lower anti-inflammatory cytokine levels may have a role in affecting the saliva secretion in psoriasis patients. Secretion of unstimulated saliva in psoriasis patients decreases with the severity and duration of the disease.","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":"71 5","pages":"1170-1180"},"PeriodicalIF":3.2,"publicationDate":"2024-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141157551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The inhibitory impact of Schisandrin on inflammation and oxidative stress alleviates LPS-induced acute kidney injury 五味子素对炎症和氧化应激的抑制作用减轻了 LPS 引起的急性肾损伤。

IF 3.2 4区生物学

Biotechnology and applied biochemistry Pub Date : 2024-05-26 DOI: 10.1002/bab.2602

Xinyao Liu, Qiuxia Huang, Wenqi Li, Jinjin Yu, Jiabao Yu, Yajie Yang, Huixin Song, Yang Liu, Xiaofeng Niu, Weifeng Li

{"title":"The inhibitory impact of Schisandrin on inflammation and oxidative stress alleviates LPS-induced acute kidney injury","authors":"Xinyao Liu, Qiuxia Huang, Wenqi Li, Jinjin Yu, Jiabao Yu, Yajie Yang, Huixin Song, Yang Liu, Xiaofeng Niu, Weifeng Li","doi":"10.1002/bab.2602","DOIUrl":"10.1002/bab.2602","url":null,"abstract":"Inflammation and oxidative stress (OS) are the major pathogenic characteristics of acute kidney injury (AKI). Studies have shown that Schisandrin (Sch) could regulate inflammatory disease. However, the function and mechanism of Sch in AKI progression are still unknown. Here, we investigated Sch's potential effects and mechanism on mice's renal damage and macrophages induced by lipopolysaccharide (LPS). Sch decreased LPS-induced inflammatory factor production while increasing the activity of related antioxidant enzymes in macrophages and mouse kidney tissues. Hematoxylin and eosin staining revealed that Sch may have the ability to profoundly inhibit inflammatory cell invasion and tissue damage caused by LPS in renal tissue. Furthermore, Western blot and immunohistochemical studies showed that Sch exerted its effects mainly through up-regulation of nuclear factor erythroid 2-related factor 2/heme oxygenase-1 and inhibition of Toll-like receptor 4‒mitogen-activated protein kinases/nuclear factor-kappa B pathways. Collectively, this study illustrates that Sch suppresses LPS-stimulated AKI by descending inflammation and OS, illuminating prospective AKI treatment options.","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":"71 5","pages":"1116-1128"},"PeriodicalIF":3.2,"publicationDate":"2024-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141154506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0