Biotechnology and applied biochemistry最新文献_第8页

Synthesis and in Silico Evaluation of Novel Triazolone-Derived Naphthalene-2-Sulfonates, Evaluation of Potential Antiproliferative Agents and Enzyme Inhibitory Activities 新型三唑酮衍生萘-2-磺酸盐的合成、硅评价、潜在抗增殖剂和酶抑制活性评价。

IF 2.5 4区生物学

Biotechnology and applied biochemistry Pub Date : 2026-08-06 Epub Date: 2026-02-05 DOI: 10.1002/bab.70140

Onur Akyıldırım, Abdülmelik Aras, Ercan Oguz, Alpaslan Bayrakdar, Fikret Türkan, Murat Beytur, Hilal Medetalibeyoğlu, Ercan Bursal, Haydar Yüksek

{"title":"Synthesis and in Silico Evaluation of Novel Triazolone-Derived Naphthalene-2-Sulfonates, Evaluation of Potential Antiproliferative Agents and Enzyme Inhibitory Activities","authors":"Onur Akyıldırım, Abdülmelik Aras, Ercan Oguz, Alpaslan Bayrakdar, Fikret Türkan, Murat Beytur, Hilal Medetalibeyoğlu, Ercan Bursal, Haydar Yüksek","doi":"10.1002/bab.70140","DOIUrl":"10.1002/bab.70140","url":null,"abstract":"<div>\u0000 \u0000 This study reports the synthesis and biological evaluation of four novel N-acetyl-derived heterocyclic compounds, namely 2-((1-acetyl-3-substituted-5-oxo-1,5-dihydro-4H-1,2,4-triazol-4-yl)iminomethyl)-phenyl-naphthalene-2-sulfonates. The compounds were synthesized via acetylation reactions using acetic anhydride and were fully characterized by IR, 1H NMR, 13C NMR, elemental analysis, and HR-MS techniques. The anticancer activities of the synthesized compounds were evaluated in the concentration range of 1.563–200 µM against HepG2 (hepatocellular carcinoma) and U87 (glioblastoma) cell lines, showing significant cytotoxic effects. Enzyme inhibition assays demonstrated potent inhibitory activities against α-glucosidase, α-amylase, acetylcholinesterase (AChE), and glutathione S-transferase (GST), with IC50 values ranging from 1.4 to 2.9 µM. Molecular docking studies, performed using DFT-optimized geometries, supported the experimental findings and revealed strong and specific interactions with the target proteins. Among the studied derivatives, compound 2a exhibited the highest affinity toward α-glucosidase, 2d showed superior binding to α-amylase and AChE, while 2c demonstrated enhanced interaction with GST. Compound 2b displayed promising anticancer potential, forming stable complexes with proteins associated with the HepG2 and U87 cell lines.\u0000 </div>","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":"73 4","pages":"1885-1898"},"PeriodicalIF":2.5,"publicationDate":"2026-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146123873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Repurposing FDA-Approved Antiviral Drugs Ganciclovir and Acyclovir Against Respiratory Syncytial Virus Nucleoprotein: Molecular Docking, ADMET, PASS, and Molecular Dynamics Simulations. 重新利用fda批准的抗病毒药物更昔洛韦和阿昔洛韦抗呼吸道合胞病毒核蛋白：分子对接，ADMET， PASS和分子动力学模拟。

IF 2.5 4区生物学

Biotechnology and applied biochemistry Pub Date : 2026-08-02 DOI: 10.1002/bab.70193

Mohammed Asiri, Mohammad Y Alshahrani, Saad Ali Alshehri, Mohammad Ali Abdullah Almoyad, Shadma Wahab

{"title":"Repurposing FDA-Approved Antiviral Drugs Ganciclovir and Acyclovir Against Respiratory Syncytial Virus Nucleoprotein: Molecular Docking, ADMET, PASS, and Molecular Dynamics Simulations.","authors":"Mohammed Asiri, Mohammad Y Alshahrani, Saad Ali Alshehri, Mohammad Ali Abdullah Almoyad, Shadma Wahab","doi":"10.1002/bab.70193","DOIUrl":"https://doi.org/10.1002/bab.70193","url":null,"abstract":"Respiratory syncytial virus (RSV) is a prevalent viral respiratory infection that has been increasingly affecting children under 5 years old globally. Although RSV can infect individuals of all ages, certain groups are at higher risk, particularly infants and young children. This growing concern underscores the urgent need for effective therapeutic options. In this context, drug repurposing emerges as a promising strategy, allowing existing medications to be utilized for new therapeutic applications beyond their original indications. In this research, we utilized library of FDA-approved antiviral drugs that show potential for repurposing. We performed molecular docking against RSV nucleoprotein. The two resulting drugs, ganciclovir and acyclovir, reveal notable binding energy and critical interactions. Further ADMET profile and prediction of activity spectra for substances (PASS) analysis show potential to be antiviral drugs against nucleoprotein. Furthermore, molecular dynamics simulations for 500 ns were conducted, along with calculations of principal component analysis (PCA) and Gibbs free energy for the most promising ligand-receptor complexes identified in docking ADMET and PASS analysis (ganciclovir and acyclovir). The simulations provided insights into their thermodynamic and dynamic properties, further investigating the docking results. The findings from this study offer valuable leads for the development of therapeutic agents against RSV. However, to substantiate the preventive and therapeutic potential of these compounds, further validations, including in vitro, animal studies, and rigorous clinical trials, are necessary.","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148663461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In Vitro Evaluation of Acetaminophen as a Repurposed Anti-Influenza Virus Agent. 对乙酰氨基酚作为新型抗流感病毒药物的体外评价。

IF 2.5 4区生物学

Biotechnology and applied biochemistry Pub Date : 2026-08-01 Epub Date: 2026-03-04 DOI: 10.1002/bab.70154

Surojit Sadhu, Sanket Bapat, Pinky Singh, Shilpa Nair, Sandeepan Mukherjee, Renu Vyas

{"title":"In Vitro Evaluation of Acetaminophen as a Repurposed Anti-Influenza Virus Agent.","authors":"Surojit Sadhu, Sanket Bapat, Pinky Singh, Shilpa Nair, Sandeepan Mukherjee, Renu Vyas","doi":"10.1002/bab.70154","DOIUrl":"10.1002/bab.70154","url":null,"abstract":"Influenza remains a major global health concern, characterized by recurrent seasonal outbreaks and the persistent threat of pandemic strains. Although vaccines and antivirals are available, their effectiveness is often compromised by antigenic variation and the emergence of drug-resistant viruses. In this study, we investigated the drug repurposing potential of FDA-approved compounds. A total of 1120 FDA-approved antiviral drugs and inhibitors were selected from publicly available databases, including PubChem and DrugBank. The 2D structures of these ligands were imported and energy-minimized using Chem3D v16.0 to ensure geometrically optimized conformations suitable for docking. The 3D crystal structures of four key influenza proteins, nucleocapsid protein (Protein Data Bank [PDB]: 2IQH), neuraminidase (PDB: 2HU4), hemagglutinin HA1 (PDB: 3UBE), and the Influenza A H5N1 PB2 cap-binding domain (PDB: 4CB4), were retrieved from the RCSB PDB and carefully prepared for docking. Molecular docking identified acetaminophen, asparagine, and acyclovir as the top three scoring compounds, with acetaminophen showing the strongest interaction at the PB2 cap-binding site. Subsequent in vitro antiviral studies were performed for all three compounds; however, asparagine and acyclovir did not exhibit significant inhibitory effects, whereas acetaminophen demonstrated promising activity. These findings suggest that acetaminophen may interfere with some key steps in the life cycle of the influenza virus. Given its established safety profile and widespread availability, acetaminophen emerges as a promising candidate for therapeutic repurposing. Nevertheless, further mechanistic and in vivo validation is essential to substantiate its potential role in influenza treatment strategies.","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":"2059-2067"},"PeriodicalIF":2.5,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13446423/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147353888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Amylase Production From Potato Peels by Bacillus sp. Under Improved Culture Conditions. 改良培养条件下芽孢杆菌从马铃薯皮中生产淀粉酶。

IF 2.5 4区生物学

Biotechnology and applied biochemistry Pub Date : 2026-08-01 Epub Date: 2026-02-25 DOI: 10.1002/bab.70149

Sourav Agarwal, Priya Mitra, Shreosee Ghosh, Kunal Vora, Tania Paul, Nishant Yadav, Abdulaziz Alangari, Rabbani Syed, Xinrui Bai, Rupak Roy

{"title":"Amylase Production From Potato Peels by Bacillus sp. Under Improved Culture Conditions.","authors":"Sourav Agarwal, Priya Mitra, Shreosee Ghosh, Kunal Vora, Tania Paul, Nishant Yadav, Abdulaziz Alangari, Rabbani Syed, Xinrui Bai, Rupak Roy","doi":"10.1002/bab.70149","DOIUrl":"10.1002/bab.70149","url":null,"abstract":"Amylase, an enzyme with significant industrial applications, can be produced sustainably and economically using agricultural waste. This study investigates amylase production from Bacillus species utilizing potato peel substrates, aiming to achieve both environmental and economic benefits. Bacterial strains capable of producing amylase were isolated and characterized. Using response surface methodology, key variables, substrate concentration, pH, and temperature, were optimized to enhance enzymatic activity. The effects of acidic and alkaline pretreatments on potato peel substrates were also assessed. Amylase was purified through dialysis and salting out and analyzed using sodium dodecyl sulfate-polyacrylamide electrophoresis (SDS-PAGE). Enzyme immobilization in calcium alginate beads was performed to evaluate stability for industrial reuse. Optimal production was achieved at a substrate concentration of 17.4%, pH 10.8, and temperature 54.2°C, with alkaline pretreatment significantly improving yield. Purification increased enzyme concentration and purity, confirmed by the presence of a distinct 55 kDa band in SDS-PAGE. Immobilization enhanced enzyme stability, indicating potential for repeated industrial applications. These findings highlight the viability of producing amylase from agricultural waste while reducing costs and environmental impact. The results also emphasize the value of substrate pretreatment and enzyme immobilization in developing efficient and sustainable biotechnological processes.","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":"1988-2004"},"PeriodicalIF":2.5,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13446433/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147302617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Constructing Haloarchaeal MVA Pathway in Serratia marcescens for Synthesis of Ocimene. 构建粘质沙雷菌合成辛烯的盐古菌MVA途径。

IF 2.5 4区生物学

Biotechnology and applied biochemistry Pub Date : 2026-08-01 Epub Date: 2026-02-26 DOI: 10.1002/bab.70151

Songsong Yao, Di Liu, Long Wang, TaiPing Fan, Huaxiang Deng, Yujie Cai

引用次数: 0

An In Silico Study of Resveratrol Derivatives as Dual Inhibitors of Cyclooxygenase-2 (COX-2) and 5-Lipoxygenase (5-LOX) in Cancer Therapy. 白藜芦醇衍生物作为环氧化酶-2 （COX-2）和5-脂氧合酶（5-LOX）双重抑制剂在癌症治疗中的硅实验研究。

IF 2.5 4区生物学

Biotechnology and applied biochemistry Pub Date : 2026-08-01 Epub Date: 2026-02-20 DOI: 10.1002/bab.70150

Sayali Doiphode, Payel Ghosh, K Venkateswara Swamy, Shuchi Nagar

{"title":"An In Silico Study of Resveratrol Derivatives as Dual Inhibitors of Cyclooxygenase-2 (COX-2) and 5-Lipoxygenase (5-LOX) in Cancer Therapy.","authors":"Sayali Doiphode, Payel Ghosh, K Venkateswara Swamy, Shuchi Nagar","doi":"10.1002/bab.70150","DOIUrl":"10.1002/bab.70150","url":null,"abstract":"Chronic inflammation plays a crucial role in cancer initiation, progression, and metastasis, with cyclooxygenase-2 (COX-2) and 5-lipoxygenase (5-LOX) often co-expressed and upregulated in tumor-associated inflammatory microenvironments. Resveratrol, a naturally occurring polyphenolic compound, has been reported to exert anticancer effects by influencing processes such as angiogenesis, apoptosis, cell proliferation, and inflammatory signaling. In this study, resveratrol derivatives were retrieved from the PubChem database using a similarity-based approach and evaluated as potential dual inhibitors of COX-2 and 5-LOX through molecular docking and 100 ns molecular dynamics (MD) simulations. The top four derivatives, FRV126, FRV169, FRV129, and FRV217, showed better docking scores than resveratrol for both targets. Analysis of MD trajectories, including root mean square deviation (RMSD), root mean square fluctuation (RMSF), hydrogen-bond profiles, and compactness (radius of gyration), indicated that these complexes remained stable throughout the simulation period. Molecular mechanics/generalized Born surface area (MM/GBSA) analysis supported strong protein-ligand interactions, whereas in silico absorption, distribution, metabolism, excretion, and toxicity (ADMET) evaluation indicated desirable pharmacokinetic and safety profiles. These findings highlight the identified resveratrol derivatives as promising dual COX-2/5-LOX inhibitors for further development in cancer therapy.","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":"2005-2021"},"PeriodicalIF":2.5,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13446436/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146257673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating a Medicinally Important Novel Species of Termitomyces From the Lateritic Region of West Bengal. 研究西孟加拉邦红土地区一具重要药用价值的白蚁新种。

IF 2.5 4区生物学

Biotechnology and applied biochemistry Pub Date : 2026-08-01 Epub Date: 2026-03-08 DOI: 10.1002/bab.70153

Rupsa Roy, Pinaki Chattopadhyay, Arghya Naskar, Krishnendu Acharya, Sanjoy Guha Roy, Arun Kumar Dutta

{"title":"Investigating a Medicinally Important Novel Species of Termitomyces From the Lateritic Region of West Bengal.","authors":"Rupsa Roy, Pinaki Chattopadhyay, Arghya Naskar, Krishnendu Acharya, Sanjoy Guha Roy, Arun Kumar Dutta","doi":"10.1002/bab.70153","DOIUrl":"10.1002/bab.70153","url":null,"abstract":"A new species, Termitomyces pseudopakistanensis, has been described from West Bengal, an often-overlooked myco-resource, despite its rich historical significance as a valuable food source. Surprisingly, it remains underappreciated in urban markets. Thus, the focus was on examining the taxon's potential health advantages to preserve indigenous culture and promote the use of locally accessible functional food. Mycochemical analysis has shown the presence of bioactive components, including lycopene, beta-carotene, flavonoids, and phenol. In addition, the ethanolic extract demonstrated strong antioxidant, iron-chelating, reducing, and anti-lipid peroxidation properties in the antioxidative assays, with an EC50 value ranging from 658.66 to 1845.079 µg/mL. The extract showed antimicrobial properties as depicted by the minimum inhibitory concentration (MIC) values ranging between 620.088 and 1528.773 µg/mL. Alkaloids, amino acid derivatives, and other secondary metabolites with substantial bioactivities are detected using liquid chromatography-mass spectrometry (LC-MS) analysis. Furthermore, the cytotoxic and apoptosis-inducing properties of the extract were investigated in the A549 cell line through a cell viability assay and other staining assays.","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":"2041-2058"},"PeriodicalIF":2.5,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13446415/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147376107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural Characterization and Anti-Inflammatory Activities of Polysaccharide From Hericium erinaceus Mycelium. 猴头菌菌丝体多糖结构表征及抗炎活性研究。

IF 2.5 4区生物学

Biotechnology and applied biochemistry Pub Date : 2026-08-01 Epub Date: 2026-04-24 DOI: 10.1002/bab.70148

Chong Sun, Liuya Wang, Yushan Shi, Xueqing Geng, Huiqing Sun, Tengda Li, Qi Zhu, Lanfeng Hui, Xiaojian Wu, Lijing Chen, Zhenyuan Zhu

引用次数: 0

Policy Intervention Towards Ecological Balance Through Reduction of Carbon Footprint in India. 通过减少印度的碳足迹实现生态平衡的政策干预。

IF 2.5 4区生物学

Biotechnology and applied biochemistry Pub Date : 2026-08-01 Epub Date: 2026-03-17 DOI: 10.1002/bab.70152

Shrabana Chattopadhyay, Aritra Jana, Shambhu Prasad Chakrabarty

{"title":"Policy Intervention Towards Ecological Balance Through Reduction of Carbon Footprint in India.","authors":"Shrabana Chattopadhyay, Aritra Jana, Shambhu Prasad Chakrabarty","doi":"10.1002/bab.70152","DOIUrl":"10.1002/bab.70152","url":null,"abstract":"India is experiencing unprecedented urbanisation and industrialisation with consequential carbon emission, which significantly increases the gap between international commitments and reality. This paper explores the recent policy initiatives aimed at achieving promised indicators and engage in recent developments on decarbonisation. The paper further identifies the gap between sustainable development and economic advancement. The paper reflects on the recent carbon-mitigating policies adopted by India. A doctrinal research strategy has been used to address this issue. International, foreign and domestic policies have been discussed to understand the impact of these policies on climate change crisis. Models from other nations were also evaluated, taking into consideration India's unique socio-economic context. This research aims to identify adaptable techniques to effectively manage emissions in critical sectors, including energy, transport and industry. The paper proposes a set of concrete policy interventions that can promote ecological balance while sustaining economic development. Once the regulatory bodies initiate appropriate implementation of these policies, the carbon emissions in India shall be more resilient and effective.","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":"2032-2040"},"PeriodicalIF":2.5,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13446419/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147472854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unraveling the Time-Dependent Effects of Ethanol on Liver Disease: Insights From a Mice Model. 揭示乙醇对肝脏疾病的时间依赖性作用：来自小鼠模型的见解。

IF 2.5 4区生物学

Biotechnology and applied biochemistry Pub Date : 2026-08-01 Epub Date: 2026-03-17 DOI: 10.1002/bab.70157

Subhasish Maity, Ayantika Santra, Gouthami Kuruvalli, Padmavathi Pannuru, Althaf Hussain Shaik, Selvam Arjunan, Vaddi Damodara Reddy

{"title":"Unraveling the Time-Dependent Effects of Ethanol on Liver Disease: Insights From a Mice Model.","authors":"Subhasish Maity, Ayantika Santra, Gouthami Kuruvalli, Padmavathi Pannuru, Althaf Hussain Shaik, Selvam Arjunan, Vaddi Damodara Reddy","doi":"10.1002/bab.70157","DOIUrl":"10.1002/bab.70157","url":null,"abstract":"Alcohol-associated liver disease (ALD) is a significant global health concern that is characterized by hepatic triglyceride accumulation and dysregulation with impairment of oxygen homeostasis. This study investigated the time-dependent effects of ethanol exposure on liver disease progression in 2-month-old male C57/BL6 mice. Mice were treated with ethanol (20% ethanol at 5 gm/kg.b.wt/day) for 2, 4, and 6 months, and blood biochemical markers, liver histopathology, and gene expression were evaluated. Results showed that ethanol exposure led to significant increases in thiobarbituric acid reactive substances (TBARS), protein carbonyls, plasma nitric oxide (NOx), C-reactive protein, and homocysteine, liver enzymes, such as aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), gamma-glutamyl transferase (γ-GT), lactate dehydrogenase (LDH) indicating oxidative stress and liver injury. Lipid profile analysis revealed increased total cholesterol and triglycerides, with decreased HDL-cholesterol. Moreover, reduced mitochondrial enzyme activity indicates dysfunction. Histopathology and qRT-PCR analysis showed increased CYP2E1, Bax, Bcl2, p53, caspase-3, caspase-9, and inducible nitric oxide synthase (iNOS) gene expression, leading to ROS/RNS generation. The miR-21 was upregulated, while miR-26a was downregulated, contributing to lipid metabolism dysregulation, pro-inflammation, and pro-fibrosis. These findings suggest that ethanol exposure causes triglyceride accumulation and cholesterol dysregulation, leading to oxidative stress, mitochondrial dysfunction, and hepatocellular injury. The dysregulation of miR-21 and miR-26a contributes to ALD progression. Markers of oxidative stress, miRNAs, and disrupted metabolic pathways may serve as potential biomarkers or therapeutic targets for early detection and intervention in ALD.","PeriodicalId":9274,"journal":{"name":"Biotechnology and applied biochemistry","volume":" ","pages":"2092-2101"},"PeriodicalIF":2.5,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13446414/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147472814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0