Breeding Science最新文献_第9页

Seed management using NGS technology to rapidly eliminate a deleterious allele from rice breeder seeds. 利用NGS技术快速消除水稻育种者种子中的有害等位基因的种子管理。

IF 2.4 4区农林科学

Breeding Science Pub Date : 2022-12-01 DOI: 10.1270/jsbbs.22058

Elias G Balimponya, Maria S Dwiyanti, Toshiaki Ito, Shuntaro Sakaguchi, Koichi Yamamori, Yoshitaka Kanaoka, Yohei Koide, Yoshifumi Nagayoshi, Yuji Kishima

{"title":"Seed management using NGS technology to rapidly eliminate a deleterious allele from rice breeder seeds.","authors":"Elias G Balimponya, Maria S Dwiyanti, Toshiaki Ito, Shuntaro Sakaguchi, Koichi Yamamori, Yoshitaka Kanaoka, Yohei Koide, Yoshifumi Nagayoshi, Yuji Kishima","doi":"10.1270/jsbbs.22058","DOIUrl":"https://doi.org/10.1270/jsbbs.22058","url":null,"abstract":"Spontaneous mutations are stochastic phenomena that occur in every population. However, deleterious mutated allele present in seeds distributed to farmers must be detected and removed. Here, we eliminated undesirable mutations from the parent population in one generation through a strategy based on next-generation sequencing (NGS). This study dealt with a spontaneous albino mutant in the 'Hinohikari' rice variety grown at the Miyazaki Comprehensive Agricultural Experiment Station, Japan. The incidence of albinism in the population was 1.36%. NGS analysis revealed the genomic basis for differences between green and albino phenotypes. Every albino plant had a C insertion in the Snow-White Leaf1 (SWL1) gene on chromosome 4 causing a frameshift mutation. Selfing plants heterozygous for the mutant allele, swl1-R332P, resulted in a 3:1 green/albino ratio, confirming that a single recessive gene controls albinism. Ultrastructural leaf features in the swl1-R332P mutants displayed deformed chlorophyll-associated organelles in albino plants that were similar to those of previously described swl1 mutants. Detection of the causative gene and its confirmation using heterozygous progenies were completed within a year. The NGS technique outlined here facilitates rapid identification of spontaneous mutations that can occur in breeder seeds.","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"72 5","pages":"362-371"},"PeriodicalIF":2.4,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9895803/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9274783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Induction of 2n pollen with colchicine during microsporogenesis in Phalaenopsis. 秋水仙碱诱导蝴蝶兰小孢子形成过程中的2n花粉。

IF 2.4 4区农林科学

Breeding Science Pub Date : 2022-09-01 DOI: 10.1270/jsbbs.21100

Ting Wu, Xin Zhao, Shuhua Yang, Jiahui Yang, Jun Zhu, Yaping Kou, Xiaonan Yu, Hong Ge, Ruidong Jia

引用次数: 1

Colletotrichum orbiculare strains distributed in Japan: race identification and evaluation of virulence to cucurbits. 分布于日本的圆形炭疽病菌:小种鉴定及对葫芦的毒力评价。

IF 2.4 4区农林科学

Breeding Science Pub Date : 2022-09-01 DOI: 10.1270/jsbbs.22011

Hiroki Matsuo, Yasuhiro Ishiga, Yasuyuki Kubo, Yosuke Yoshioka

{"title":"Colletotrichum orbiculare strains distributed in Japan: race identification and evaluation of virulence to cucurbits.","authors":"Hiroki Matsuo, Yasuhiro Ishiga, Yasuyuki Kubo, Yosuke Yoshioka","doi":"10.1270/jsbbs.22011","DOIUrl":"https://doi.org/10.1270/jsbbs.22011","url":null,"abstract":"The pathogen Colletotrichum orbiculare is causal fungus of cucurbit anthracnose. Multiple races have been identified in the United States, suggesting that it is necessary to cultivate suitable resistant cultivars and breed new cultivars with the most suitable resistance gene. This study examined the pathogenicity and virulence of 20 strains in Japan to clarify the existence of races and virulence differences. Based on the symptoms on inoculated cotyledons and true leaves of watermelon, we could evaluate the compatibility of each strain to each host cultivar. Our analysis based on the reaction to the host cultivar harboring the resistance gene Ar-1 (Cla001017) revealed the existence of three races in Japan. An alarming result was that a race that overcame Ar-1, which is a target gene in current watermelon breeding in Japan, is present in Japan. The cucumber and melon host cultivars showed diverse symptoms, whereas a squash cultivar was resistant to all strains. Three strains caused severe damage even to the most resistant cucumber cultivar 'Ban Kyuri' and resistant cultivars harboring Cssgr, a well-known gene conferring loss-of-susceptibility resistance. Screening genetic resources for novel resistance genes using strains with high virulence is of vital importance for watermelon, cucumber, and melon production.","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"72 4","pages":"306-315"},"PeriodicalIF":2.4,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9868334/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10623253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Genomic markers analysis associated with resistance to Alternaria alternata (fr.) keissler-tomato pathotype, Solanum lycopersicum L. 茄疫病(Solanum lycopersicum L.)抗性的基因组标记分析。

IF 2.4 4区农林科学

Breeding Science Pub Date : 2022-09-01 DOI: 10.1270/jsbbs.22003

Giti Alizadeh-Moghaddam, Mehdi Nasr-Esfahani, Zahra Rezayatmand, Mahdi Khozaei

{"title":"Genomic markers analysis associated with resistance to Alternaria alternata (fr.) keissler-tomato pathotype, Solanum lycopersicum L.","authors":"Giti Alizadeh-Moghaddam, Mehdi Nasr-Esfahani, Zahra Rezayatmand, Mahdi Khozaei","doi":"10.1270/jsbbs.22003","DOIUrl":"https://doi.org/10.1270/jsbbs.22003","url":null,"abstract":"Alternaria alternata, the causal pathogen of early blight (EB) disease, is one of the most important diseases in tomato, and other solanaceae family. We analyzed 35 tomato genotypes for quantitative/qualitative traits and biomass growth parameters, as well as the extent and structure of genetic variation associated with EB resistance. Phenotypic comparisons displayed significant differences in leaf blade width (24.95%), stem thickness (30.28%), foliage density (18.88%), and plant size (18.89%), with significant positive correlations with EB resistance (0.18-0.75). Correlation analysis showed that mature fruit size, thickness of fruit pericarp, and leaf type were significantly and negatively correlated with EB resistance (up to -0.41). The susceptible tomato seedlings represented significant reductions in biomass parameters. According to ISSR analysis, the highest resolving power (≥0.79) and heterozygosity (≥0.24) values revealed the presence of high genetic variability among the tomato genotypes. Bayesian model-based STRUCTURE analysis assembled the genotypes into 4 (best ΔK = 4) genetic groups. Combined phenotypic and molecular markers proved to be significantly useful for genetic diversity assessment associated with EB disease resistance.","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"72 4","pages":"285-296"},"PeriodicalIF":2.4,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9868332/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9535095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Wheat yellow mosaic virus resistant line, 'Kitami-94', developed by introgression of two resistance genes from the cultivar 'Madsen'. 小麦黄花叶病毒抗性品系‘Kitami-94’是通过从品种‘Madsen’中引入两个抗性基因而获得的。

IF 2.4 4区农林科学

Breeding Science Pub Date : 2022-09-01 DOI: 10.1270/jsbbs.21101

Takako Suzuki, Yasuhiro Yoshimura, Shizen Ohnishi, Hironobu Jinno, Tatsuya Sonoda, Masashi Kasuya, Chihiro Souma, Tetsuya Inoue, Masatomo Kurushima, Akira Sugawara, Shinji Maeno, Takao Komatsuda

{"title":"Wheat yellow mosaic virus resistant line, 'Kitami-94', developed by introgression of two resistance genes from the cultivar 'Madsen'.","authors":"Takako Suzuki, Yasuhiro Yoshimura, Shizen Ohnishi, Hironobu Jinno, Tatsuya Sonoda, Masashi Kasuya, Chihiro Souma, Tetsuya Inoue, Masatomo Kurushima, Akira Sugawara, Shinji Maeno, Takao Komatsuda","doi":"10.1270/jsbbs.21101","DOIUrl":"https://doi.org/10.1270/jsbbs.21101","url":null,"abstract":"'Kitahonami' is a soft red winter wheat (Triticum aestivum L.) cultivar that has high yield, good agronomic performance and good quality characteristics. It currently accounts for 73% of the wheat cultivation area of Hokkaido the northern island in Japan and 42% of Japan's overall wheat cultivation. However, this cultivar is susceptible to Wheat yellow mosaic virus (WYMV). WYMV has become widespread recently, with serious virus damage reported in Tokachi and Ohotsuku districts, which are the main wheat production areas in Hokkaido. Here, we report a new wheat breeding line 'Kitami-94', which was developed over four years by repeated backcrossing with 'Kitahonami' using DNA markers for WYMV resistance linked to the Qym1 and Qym2 from 'Madsen'. Basic maps of Qym1 and Qym2 were created and used to confirm that 'Kitami-94' reliably carried the two resistance genes. 'Kitami-94' demonstrated WYMV resistance, and had agronomic traits and quality equivalent to 'Kitahonami' except for higher polyphenol oxidase activity and lower thousand grain weight. 'Kitami-94' may be useful for elucidating the mechanism of WYMV resistance in the background of 'Kitahonami', and for developing new cultivars.","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"72 4","pages":"297-305"},"PeriodicalIF":2.4,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9868331/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10677655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Effects of dwarfing allele sd1-d originating from 'Dee-geo-woo-gen' and its tall alleles SD1-in and SD1-ja on morphological characteristics concerning dry-matter production and photosynthesis on the genetic background of indica-rice IR36. 源自“dee -geo- wood -gen”的矮化等位基因sd1-d及其高等位基因SD1-in和SD1-ja对籼稻IR36干物质生产和光合形态特征的影响

IF 2.4 4区农林科学

Breeding Science Pub Date : 2022-09-01 DOI: 10.1270/jsbbs.22016

Misa Kamimukai, Birendra Bahadur Rana, Mukunda Bhattarai, Masayuki Murai

{"title":"Effects of dwarfing allele sd1-d originating from 'Dee-geo-woo-gen' and its tall alleles SD1-in and SD1-ja on morphological characteristics concerning dry-matter production and photosynthesis on the genetic background of indica-rice IR36.","authors":"Misa Kamimukai, Birendra Bahadur Rana, Mukunda Bhattarai, Masayuki Murai","doi":"10.1270/jsbbs.22016","DOIUrl":"https://doi.org/10.1270/jsbbs.22016","url":null,"abstract":"sd1-d has been utilized to develop short-culmed indica varieties adaptable to higher fertilizer-applications. Its tall alleles SD1-in and SD1-ja are harbored in indica and japonica subspecies, respectively. SD1-in possesses a higher effect on elongating culm than SD1-ja. The sd1-d of indica IR36 was substituted with SD1-in or SD1-ja through recurrent backcrossing with IR36, and two tall isogenic lines (\"5867-36\" and \"Koshi-36\") were developed. IR36, 5867-36 and Koshi-36 were grown in a paddy field, and the effects of sd1-d, SD1-in and SD1-ja on morphological characteristics concerning dry-matter production and photosynthesis were compared mutually. sd1-d diminished dry weight of total brown rice/m2 and total dry matter weights, but enhanced harvest indexes, compared with SD1-in. In IR36, shorter lengths of the first (flag) to third leaves, and more panicle-bearing stems, caused by sd1-d, compared with SD1-in-carrying 5867-36, and erect first leaves, not caused by sd1-d, could construct the canopy structure appropriate for obtaining a high rate of photosynthesis at an optimum LAI. Koshi-36 could be used for a mid-mother line to develop indica varieties adaptable to middle and low fertilizer-applications, due to higher effect of SD1-ja on yielding ability, compared with that of sd1-d, no breaking-type lodging, and resistances to diseases and pests.","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"72 4","pages":"316-325"},"PeriodicalIF":2.4,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9868333/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10677657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diversity of Na⁺ allocation in salt-tolerant species of the genus Vigna. 耐盐油菜品种Na+分配的多样性。

IF 2.4 4区农林科学

Breeding Science Pub Date : 2022-09-01 DOI: 10.1270/jsbbs.22012

Yusaku Noda, Ryohei Sugita, Atsushi Hirose, Naoki Kawachi, Keitaro Tanoi, Jun Furukawa, Ken Naito

{"title":"Diversity of Na+ allocation in salt-tolerant species of the genus Vigna.","authors":"Yusaku Noda, Ryohei Sugita, Atsushi Hirose, Naoki Kawachi, Keitaro Tanoi, Jun Furukawa, Ken Naito","doi":"10.1270/jsbbs.22012","DOIUrl":"https://doi.org/10.1270/jsbbs.22012","url":null,"abstract":"Wild species in the genus Vigna are a great resource of tolerance to various stresses including salinity. We have previously screened the genetic resources of the genus Vigna and identified several accessions that have independently evolved salt tolerance. However, many aspects of such tolerance have remained unknown. Thus, we used autoradiography with radioactive sodium (22Na+) and Inductively Coupled Plasma Mass Spectrometry (ICP-MS) to visualize and compare Na+ allocation in Vigna angularis (Willd.) Ohwi & H.Ohashi (azuki bean), Vigna nakashimae (Ohwi) Ohwi & H.Ohashi, Vigna riukiuensis (Ohwi) Ohwi & H.Ohashi, Vigna luteola (Jacq.) Benth. and Vigna marina (Burm.) Merr.. The results indicated: 1) Tolerant accessions suppress Na+ accumulation compared to azuki bean. 2) V. nakashimae and V. marina does so by accumulating higher amount of K+, whereas V. riukiuensis and V. luteola does so by other mechanisms. 3) V. luteola avoids salt-shedding by allocating excess Na+ to newly expanded leaves. As the mechanisms of the tolerant species were different, they could be piled up in a single crop via classical breeding or by genetic engineering or genome editing.","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"72 4","pages":"326-331"},"PeriodicalIF":2.4,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9868329/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10677658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Developing core marker sets for effective genomic-assisted selection in wheat and barley breeding programs. 开发小麦和大麦育种计划中有效的基因组辅助选择的核心标记集。

IF 2.4 4区农林科学

Breeding Science Pub Date : 2022-06-01 Epub Date: 2022-06-29 DOI: 10.1270/jsbbs.22004

Goro Ishikawa, Hiroaki Sakai, Nobuyuki Mizuno, Elena Solovieva, Tsuyoshi Tanaka, Kazuki Matsubara

{"title":"Developing core marker sets for effective genomic-assisted selection in wheat and barley breeding programs.","authors":"Goro Ishikawa, Hiroaki Sakai, Nobuyuki Mizuno, Elena Solovieva, Tsuyoshi Tanaka, Kazuki Matsubara","doi":"10.1270/jsbbs.22004","DOIUrl":"https://doi.org/10.1270/jsbbs.22004","url":null,"abstract":"Wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.) are widely cultivated temperate crops. In breeding programs with these crops in Japan, effective genomic-assisted selection was performed by selecting core marker sets from thousands of genome-wide amplicon sequencing markers. The core sets consist of 768 and 960 markers for barley and wheat, respectively. These markers are distributed evenly across the genomes and effectively detect widely distributed polymorphisms in the chromosomes. The core set utility was assessed using 1,032 barley and 1,798 wheat accessions across the country. Minor allele frequency and chromosomal distributions showed that the core sets could effectively capture polymorphisms across the entire genome, indicating that the core sets are applicable to highly-related advanced breeding materials. Using the core sets, we also assessed the trait value predictability. As observed via fivefold cross-validation, the prediction accuracies of six barley traits ranged from 0.56-0.74 and 0.62 on average, and the corresponding values for eight wheat traits ranged from 0.44-0.83 and 0.65 on average. These data indicate that the established core marker sets enable breeding processes to be accelerated in a cost-effective manner and provide a strong foundation for further research on genomic selection in crops.","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"72 3","pages":"257-266"},"PeriodicalIF":2.4,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9653188/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40488226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Identification of a unique allele in the quantitative trait locus for crown root number in japonica rice from Japan using genome-wide association studies. 利用全基因组关联研究鉴定日本粳稻冠根数数量性状位点上的一个独特等位基因。

IF 2.4 4区农林科学

Breeding Science Pub Date : 2022-06-01 Epub Date: 2022-07-01 DOI: 10.1270/jsbbs.22010

Shota Teramoto, Masanori Yamasaki, Yusaku Uga

{"title":"Identification of a unique allele in the quantitative trait locus for crown root number in japonica rice from Japan using genome-wide association studies.","authors":"Shota Teramoto, Masanori Yamasaki, Yusaku Uga","doi":"10.1270/jsbbs.22010","DOIUrl":"https://doi.org/10.1270/jsbbs.22010","url":null,"abstract":"To explore the genetic resources that could be utilized to help improve root system architecture phenotypes in rice (Oryza sativa), we have conducted genome-wide association studies to investigate maximum root length and crown root number in 135 10-day-old Japanese rice accessions grown hydroponically. We identified a quantitative trait locus for crown root number at approximately 32.7 Mbp on chromosome 4 and designated it qNCR1 (quantitative trait locus for Number of Crown Root 1). A linkage disequilibrium map around qNCR1 suggested that three candidate genes are involved in crown root number: a cullin (LOC_Os04g55030), a gibberellin 20 oxidase 8 (LOC_Os04g55070), and a cyclic nucleotide-gated ion channel (LOC_Os04g55080). The combination of haplotypes for each gene was designated as a haploblock, and haploblocks 1, 2, and 3 were defined. Compared to haploblock 1, the accessions with haploblocks 2 and 3 had fewer crown roots; approximately 5% and 10% reductions in 10-day-old plants and 15% and 25% reductions in 42-day-old plants, respectively. A Japanese leading variety Koshihikari and its progenies harbored haploblock 3. Their crown root number could potentially be improved using haploblocks 1 and 2.","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"72 3","pages":"222-231"},"PeriodicalIF":2.4,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9653191/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40477416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

A large deletion within intron 20 sequence of single-copy PolA1 gene as a useful marker for the speciation in Oryza AA-genome species. 单拷贝PolA1基因内含子20序列的大缺失作为水稻aa基因组物种物种形成的有用标记。

IF 2.4 4区农林科学

Breeding Science Pub Date : 2022-06-01 Epub Date: 2022-07-12 DOI: 10.1270/jsbbs.21075

Aung Htut Htet, So Makabe, Hiroko Takahashi, Poku Aduse Samuel, Yo-Ichiro Sato, Ikuo Nakamura

{"title":"A large deletion within intron 20 sequence of single-copy PolA1 gene as a useful marker for the speciation in Oryza AA-genome species.","authors":"Aung Htut Htet, So Makabe, Hiroko Takahashi, Poku Aduse Samuel, Yo-Ichiro Sato, Ikuo Nakamura","doi":"10.1270/jsbbs.21075","DOIUrl":"https://doi.org/10.1270/jsbbs.21075","url":null,"abstract":"Oryza AA-genome complex comprises five wild species, O. rufipogon, O. barthii, O. longistaminata, O. glumaepatula, and O. meridionalis. Evolutionary relationships among these five wild species have remained contentious and inconclusive. We found that intron 20 of PolA1, a single-copy nuclear gene, was short (S-type: 141-142 bp) in O. rufipogon, O. barthii, and O. glumaepatula, while long (L-type: ca. 1.5 kb) introns were apparent in O. longistaminata and O. meridionalis. Because Oryza species containing BB, CC, EE, FF, and GG genome showed L-type introns, the S-type intron was probably derived from the L-type intron by the deletion of a 1.4 kb fragment through intramolecular homologous recombination between two tandem TTTTGC repeats. Excluding the large deletion sequence, intron 20 sequence of O. barthii was identical to that of O. longistaminata. As more than 3,470 accessions of O. rufipogon and O. sativa also contained the same intron 20 sequence with O. longistaminata except for single T-nucleotide deletion, which was shared with O. glumaepatuala, the deletion of the T-nucleotide probably occurred in the L-type intron 20 of O. logistaminata. Deletions of a large 1.4 kb fragment and single T-nucleotide within the intron 20 of PolA1 gene were considered as useful DNA markers to study the evolutionary relationships among Oryza AA-genome species.","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"72 3","pages":"267-273"},"PeriodicalIF":2.4,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9653197/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40477417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0