Near-isogenic lines for resistance to brown planthopper with the genetic background of Indica Group elite rice (Oryza sativa L.) variety 'IR64'.

IF 2 4区农林科学 Q2 AGRONOMY

Breeding Science Pub Date : 2023-06-01 Epub Date: 2023-06-15 DOI:10.1270/jsbbs.22093

Md Mostofa Kamal, Cuong Dinh Nguyen, Sachiyo Sanada-Morimura, Shao-Hui Zheng, Daisuke Fujita

{"title":"Near-isogenic lines for resistance to brown planthopper with the genetic background of Indica Group elite rice (Oryza sativa L.) variety 'IR64'.","authors":"Md Mostofa Kamal, Cuong Dinh Nguyen, Sachiyo Sanada-Morimura, Shao-Hui Zheng, Daisuke Fujita","doi":"10.1270/jsbbs.22093","DOIUrl":null,"url":null,"abstract":"The brown planthopper (BPH), Nilaparvata lugens Stål, is an insect pest that severely damages rice (Oryza sativa L.) in Asia, causing huge yield loss. Use of resistant variety is a cost-effective and eco-friendly strategy for maintaining BPH populations below the economic injury level. However, current BPH populations have been changed to virulence against resistant varieties. In this study, to estimate effective combinations among eight BPH resistance genes (BPH32, BPH17-ptb, BPH20, BPH17, BPH3, BPH25, BPH26 and qBPH6), eight near-isogenic lines with the genetic background of an Indica Group rice variety 'IR64' (IR64-NIL) were developed using marker-assisted selection. The genome recoveries of these NILs ranged from 89.3% to 98.8% and agronomic traits of them were similar to those of 'IR64'. In modified seed box screening test, resistance level of IR64-NILs was higher than that of 'IR64'. In antibiosis test, high adult mortalities of BPH (from 56.0% to 97.0%) were observed among NILs, in comparison with that of 'IR64'. Among IR64-NILs, the line carrying BPH17 showed the highest resistance level at all tests. Thus, these IR64-NILs with multiple BPH resistance genes could be valuable breeding lines for enhancing resistance levels by gene pyramiding and multiline variety.","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"73 3","pages":"278-289"},"PeriodicalIF":2.0000,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10570883/pdf/73_278.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Breeding Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1270/jsbbs.22093","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/6/15 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"AGRONOMY","Score":null,"Total":0}

引用次数: 0

Abstract

The brown planthopper (BPH), Nilaparvata lugens Stål, is an insect pest that severely damages rice (Oryza sativa L.) in Asia, causing huge yield loss. Use of resistant variety is a cost-effective and eco-friendly strategy for maintaining BPH populations below the economic injury level. However, current BPH populations have been changed to virulence against resistant varieties. In this study, to estimate effective combinations among eight BPH resistance genes (BPH32, BPH17-ptb, BPH20, BPH17, BPH3, BPH25, BPH26 and qBPH6), eight near-isogenic lines with the genetic background of an Indica Group rice variety 'IR64' (IR64-NIL) were developed using marker-assisted selection. The genome recoveries of these NILs ranged from 89.3% to 98.8% and agronomic traits of them were similar to those of 'IR64'. In modified seed box screening test, resistance level of IR64-NILs was higher than that of 'IR64'. In antibiosis test, high adult mortalities of BPH (from 56.0% to 97.0%) were observed among NILs, in comparison with that of 'IR64'. Among IR64-NILs, the line carrying BPH17 showed the highest resistance level at all tests. Thus, these IR64-NILs with multiple BPH resistance genes could be valuable breeding lines for enhancing resistance levels by gene pyramiding and multiline variety.

查看原文本刊更多论文

以籼稻（Oryza sativa L.）优良品种‘IR64’为遗传背景的抗褐飞虱近等基因系。

褐飞虱（BPH）是一种严重危害亚洲水稻（Oryza sativa l.）的害虫，造成巨大的产量损失。使用抗性品种是一种成本效益高、环境友好的策略，可以将BPH种群保持在经济伤害水平以下。然而，目前的褐飞虱种群已经改变为对抗性品种的毒力。在本研究中，为了估计8个抗褐飞虱基因（BPH32、BPH17-ptb、BPH20、BPH17、BPH3、BPH25、BPH26和qBPH6）之间的有效组合，利用标记辅助选择法建立了8个具有籼稻品种“IR64”（IR64-NIL）遗传背景的近等基因系。这些NIL的基因组回收率在89.3%至98.8%之间，其农艺性状与“IR64”相似。在改良种箱筛选试验中，IR64 NILs的抗性水平高于‘IR64’。在抗菌试验中，与“IR64”相比，NIL中BPH的成人死亡率较高（从56.0%到97.0%）。在IR64 NIL中，携带BPH17的品系在所有测试中表现出最高的抗性水平。因此，这些具有多个BPH抗性基因的IR64 NIL可能是通过基因聚合和多系品种提高抗性水平的有价值的育种品系。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Breeding Science 农林科学-农艺学

CiteScore

4.90

自引率

4.20%

发文量

审稿时长

1.5 months

期刊介绍： Breeding Science is published by the Japanese Society of Breeding. Breeding Science publishes research papers, notes and reviews related to breeding. Research Papers are standard original articles. Notes report new cultivars, breeding lines, germplasms, genetic stocks, mapping populations, database, software, and techniques significant and useful for breeding. Reviews summarize recent and historical events related breeding. Manuscripts should be submitted by corresponding author. Corresponding author must have obtained permission from all authors prior to submission. Correspondence, proofs, and charges of excess page and color figures should be handled by the corresponding author.