Breeding Science最新文献

Polyphyletic domestication and inter-lineage hybridization magnified genetic diversity of cultivated melon, Cucumis melo L. 多系驯化和系间杂交扩大了栽培甜瓜的遗传多样性。

IF 2.2 4区农林科学

Breeding Science Pub Date : 2025-06-01 Epub Date: 2025-06-10 DOI: 10.1270/jsbbs.24045

Katsunori Tanaka, Gentaro Shigita, Tran Phuong Dung, Phan Thi Phuong Nhi, Mami Takahashi, Yuki Monden, Hidetaka Nishida, Ryuji Ishikawa, Kenji Kato

{"title":"Polyphyletic domestication and inter-lineage hybridization magnified genetic diversity of cultivated melon, Cucumis melo L.","authors":"Katsunori Tanaka, Gentaro Shigita, Tran Phuong Dung, Phan Thi Phuong Nhi, Mami Takahashi, Yuki Monden, Hidetaka Nishida, Ryuji Ishikawa, Kenji Kato","doi":"10.1270/jsbbs.24045","DOIUrl":"10.1270/jsbbs.24045","url":null,"abstract":"Melon accessions with diverse geographical origins were classified into large and small seed-types by length of seed at the boundary of 9 mm, and into five populations based on polymorphisms in the nuclear genome. They were further divided into three maternal lineages, Ia, Ib, and Ic, by polymorphisms in the chloroplast genome. By combining these three classifications, the Europe/US subsp. melo and the East Asian subsp. agrestis were characterized as [large seed, Ib, PopA1 or A2] and [small seed, Ia, PopB1 or B2], respectively, indicating nearly perfect divergence. In South Asia, in addition to the Europe/US and East Asian types, recombinant types between the two types were detected and accounted for 34.8% of South Asian melon. The finding of such an intermixed structure of genetic variation supported the Indian origin of Ia and Ib types. As to Momordica popular in South Asia, seed length was intermediate between the large and small seed-types, and chloroplast type was a mixture of Ia and Ib, suggesting its origin from the recombinant type. In Africa, three lineages of melon were distributed allopatrically and showed distinct divergence. Subsp. agrestis of the Ic type proved to be endemic to Africa, indicating its African origin.","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"75 3","pages":"168-178"},"PeriodicalIF":2.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12457790/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145147861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Leaf color variation mechanism of the yellow-to-green mutant 'ytg-2' in Phaseolus vulgaris L. 菜豆黄绿突变体ytg-2叶片颜色变异机理研究。

IF 2.2 4区农林科学

Breeding Science Pub Date : 2025-06-01 Epub Date: 2025-06-11 DOI: 10.1270/jsbbs.24018

Ziyan Wang, Dajun Liu, Xiaoxu Yang, Ruijie Ji, Guojun Feng, Zhishan Yan, Chang Liu

{"title":"Leaf color variation mechanism of the yellow-to-green mutant 'ytg-2' in Phaseolus vulgaris L.","authors":"Ziyan Wang, Dajun Liu, Xiaoxu Yang, Ruijie Ji, Guojun Feng, Zhishan Yan, Chang Liu","doi":"10.1270/jsbbs.24018","DOIUrl":"10.1270/jsbbs.24018","url":null,"abstract":"Snap bean leaves are the primary site of photosynthesis. Mutant leaf color provides valuable tools for investigating leaf color changes, their role in photosynthesis, and pigment metabolic pathways. In this study, we found that the seedling leaves of 'ytg-2' exhibited a yellow phenotype characterized by reduced chlorophyll content compared with 'A18'. Blockage of the uroporphyrinogen III (Urogen III) to the fecal porphyrinogen III (Coprogen III) synthesis pathway primarily affected the synthesis of chlorophyll intermediate metabolites. As the plants matured, their leaves transformed from yellow to light green, accompanied by an increase in the total chlorophyll content. Transcriptome analysis revealed that the significantly down-regulated expression of four genes in the HemE gene family (Phvul.008G059400.1, Phvul.010G110900.1, Phvul.008G147200.1, and Phvul.010G069500.1), which blocked the Urogen III to Coprogen III conversion, was the primary cause of the yellow phenotype in 'ytg-2' seedlings. Additionally, the down-regulation of POR (Phvul.004G113000.1) and NOL (Phvul.004G163900) genes during the chlorophyll cycle explained the reduced total chlorophyll content in 'ytg-2' and the gradual normalization of the chlorophyll a/b ratio in 'ytg-2' yellow leaves. The expression of PAO (Phvul.004G163900.1), a key enzyme in chlorophyll degradation, further confirmed that the yellow-to-green transition in 'ytg-2' leaves was linked to chlorophyll degradation processes.","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"75 3","pages":"155-167"},"PeriodicalIF":2.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12457786/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145147870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and interlaboratory validation of a cultivar-specific identification method for the table grape 'Shine Muscat' using loop-mediated isothermal amplification (LAMP). 利用环介导等温扩增技术（LAMP）对鲜食葡萄“Shine Muscat”品种特异性鉴定方法的建立和实验室间验证。

IF 2.2 4区农林科学

Breeding Science Pub Date : 2025-06-01 Epub Date: 2025-06-21 DOI: 10.1270/jsbbs.24074

Reona Takabatake, Yuki Monden, Akiko Shindo, Yasutaka Minegishi, Fumiya Taniguchi, Yu Hashimoto, Tomoyuki Takeuchi, Kazuto Takasaki, Sachiko Isobe

{"title":"Development and interlaboratory validation of a cultivar-specific identification method for the table grape 'Shine Muscat' using loop-mediated isothermal amplification (LAMP).","authors":"Reona Takabatake, Yuki Monden, Akiko Shindo, Yasutaka Minegishi, Fumiya Taniguchi, Yu Hashimoto, Tomoyuki Takeuchi, Kazuto Takasaki, Sachiko Isobe","doi":"10.1270/jsbbs.24074","DOIUrl":"10.1270/jsbbs.24074","url":null,"abstract":"'Shine Muscat' is an elite table grape cultivar developed by the National Agriculture and Food Research Organization in Japan. Recently, the infringement of breeders' rights in various fruits has become a serious problem in Japan. In this study, a loop-mediated isothermal amplification (LAMP)-mediated cultivar identification method for 'Shine Muscat' was developed. We comprehensively analyzed retrotransposon insertion sites using 24 major grape cultivars and identified two insertions, VINE1-Cl160 and VINE1-Cl155, which are unique to 'Shine Muscat'. LAMP primers targeting VINE1-Cl160 and VINE1-Cl155 were designed, and specific amplifications were confirmed. We also designed a primer set to detect the grape endogenous reference sequence, UDP-glucose:flavonoid 3-O-glucosyltransferase. To improve rapidness and cost-effectiveness, we applied single-stranded tag hybridization on a chromatography printed-array strip system, a lateral flow DNA chromatography technology. The developed method was validated with an interlaboratory study. This novel identification method would be particularly useful for border inspections.","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"75 3","pages":"200-209"},"PeriodicalIF":2.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12457787/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145147884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pollen parent affects rutin content of seeds of buckwheat (Fagopyrum esculentum). 花粉亲本对荞麦种子芦丁含量的影响。

IF 2.2 4区农林科学

Breeding Science Pub Date : 2025-06-01 Epub Date: 2025-06-18 DOI: 10.1270/jsbbs.24085

Shiori Otsuka, Takashi Hara, Koji Ishiguro, Kenichi Matsushima, Yasuo Yasui, Katsuhiro Matsui

{"title":"Pollen parent affects rutin content of seeds of buckwheat (Fagopyrum esculentum).","authors":"Shiori Otsuka, Takashi Hara, Koji Ishiguro, Kenichi Matsushima, Yasuo Yasui, Katsuhiro Matsui","doi":"10.1270/jsbbs.24085","DOIUrl":"10.1270/jsbbs.24085","url":null,"abstract":"Buckwheat (Fagopyrum esculentum) is a heterostylous self-incompatible crop that requires outcrossing for seed formation. Pollen parents influence the traits of seeds in many plants, but their influence in buckwheat is unknown. Here, we crossed self-incompatible (SI) and self-compatible (SC) lines with different rutin contents. The average rutin contents of SI leading cultivars were all 0.15-0.21 mg/g, and a SI high rutin content line that we had developed was 0.6 mg/g, although it has wide range SDs (0.12-0.41). On the other hand, the average rutin contents of SC lines were 0.01-0.06 mg/g, with stable SDs (0.02-0.03). In crosses between high- and low-rutin parents, the average rutin content of F1 seeds was significantly lower than that of the high-rutin parent and higher than that of the low-rutin parent, indicating that the pollen parent influences the rutin content in seeds of buckwheat. RNA-seq analysis confirmed that alleles of several genes encoding enzymes involved in rutin synthesis derived from pollen parents were expressed during seed formation.","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"75 3","pages":"179-186"},"PeriodicalIF":2.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12457789/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145147868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic dissection of soybean lodging tolerance in recombinant inbred-line populations of major Japanese and modern US varieties. 日本和美国主要品种重组自交系群体大豆抗倒伏性的遗传剖析。

IF 2.2 4区农林科学

Breeding Science Pub Date : 2025-06-01 Epub Date: 2025-06-21 DOI: 10.1270/jsbbs.24088

Ai Hishinuma, Atsunori Fukuda, Takuma Sugimoto, Osamu Uchikawa, Shigeki Morita, Ryohei Okuno, Shin Kato, Akio Kikuchi, Takashi Sayama, Yuko Yokota, Takehiko Shimizu, Fumio Taguchi-Shiobara, Eri Ogiso-Tanaka, Akito Kaga, Kaori Hirata, Tetsuya Yamada, Kenichiro Fujii, Feng Li, Makita Hajika, Masao Ishimoto

{"title":"Genetic dissection of soybean lodging tolerance in recombinant inbred-line populations of major Japanese and modern US varieties.","authors":"Ai Hishinuma, Atsunori Fukuda, Takuma Sugimoto, Osamu Uchikawa, Shigeki Morita, Ryohei Okuno, Shin Kato, Akio Kikuchi, Takashi Sayama, Yuko Yokota, Takehiko Shimizu, Fumio Taguchi-Shiobara, Eri Ogiso-Tanaka, Akito Kaga, Kaori Hirata, Tetsuya Yamada, Kenichiro Fujii, Feng Li, Makita Hajika, Masao Ishimoto","doi":"10.1270/jsbbs.24088","DOIUrl":"10.1270/jsbbs.24088","url":null,"abstract":"In soybean production, lodging poses a significant challenge to modern mechanized agriculture, such as the use of combine harvesters. Most Japanese varieties are prone to lodging because of the local weather conditions, such as wind and rain, resulting in a decline in productivity. In the United States (US), where mechanized agricultural production systems are prevalent, lodging tolerance (LT) is essential in soybean breeding. We thus used two recombinant inbred-line (RIL) populations developed by crossing major Japanese and modern US varieties for the genetic dissection of LT. One reliable quantitative trait locus (QTL) for lodging angle, qLT13-1, was identified from the first RIL population under two experimental conditions, early and late maturity groups of the first RILs in Ibaraki in 2018, and it accounted for 20.7%-20.9% of the phenotypic variation. An allele at qLT13-1 from a US variety was effective in improving LT under most experimental conditions. In addition, a QTL for LT was valid in the same genetic region of the other RIL populations. The effective allele, qLT13-1 is thus expected to be important for improving LT in soybean breeding, particularly in Japanese varieties.","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"75 3","pages":"224-235"},"PeriodicalIF":2.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12457785/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145147880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic control of seed iron and zinc concentration in Rwandan common bean population revealed by the Genome Wide Association Study (GWAS). 全基因组关联研究（GWAS）揭示的卢旺达普通豆群体种子铁和锌浓度的遗传控制。

IF 2.2 4区农林科学

Breeding Science Pub Date : 2025-06-01 Epub Date: 2025-06-18 DOI: 10.1270/jsbbs.24087

Floride Mukamuhirwa, Kenta Shirasawa, Ken Naito, Edouard Rurangwa, Viateur Ndayizeye, Alphonse Nyombayire, Jean Pierre Muhire, Mahalingam Govindaraj, Norikuni Ohtake, Keiichi Okazaki, Moeko Okada, Eigo Fukai

{"title":"Genetic control of seed iron and zinc concentration in Rwandan common bean population revealed by the Genome Wide Association Study (GWAS).","authors":"Floride Mukamuhirwa, Kenta Shirasawa, Ken Naito, Edouard Rurangwa, Viateur Ndayizeye, Alphonse Nyombayire, Jean Pierre Muhire, Mahalingam Govindaraj, Norikuni Ohtake, Keiichi Okazaki, Moeko Okada, Eigo Fukai","doi":"10.1270/jsbbs.24087","DOIUrl":"10.1270/jsbbs.24087","url":null,"abstract":"Common bean (Phaseolus vulgaris L.) is one of the most abundantly consumed legume crops as foods worldwide. In many African countries, this crop is an important staple food because of its rich nutrients. The Great Lakes region of Central Africa, which includes Rwanda, the nation with the highest per capita consumption of common beans worldwide, is known to be a center of common bean diversity in Africa. Increasing the amount of iron and zinc in common bean for biofortification has been a key breeding goal in Rwanda and other countries. In this study, using 192 accessions, including local landraces from Rwanda, breeding materials, released varieties, and others, we performed genome wide association studies (GWAS) to determine the loci governing those traits in addition to other agronomic traits. We identified a locus that was strongly associated with seed zinc concentration and candidate genes. The information might be a great help for marker-assisted breeding of this trait in common bean.","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"75 3","pages":"187-199"},"PeriodicalIF":2.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12457783/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145147889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel chromosome segment substitution lines derived from japonica cultivar 'Yukihikari' in the genetic background of 'Joiku462' cultivar and identification of quantitative trait loci for heading date and grain quality. 粳稻品种“久库462”遗传背景下的新染色体片段代换系及抽穗期和品质数量性状位点的鉴定

IF 2.2 4区农林科学

Breeding Science Pub Date : 2025-06-01 Epub Date: 2025-06-18 DOI: 10.1270/jsbbs.24058

Kiyoaki Kato, Shinya Munekata, Toshiro Watanabe, Takashi Sato, Yusuke Hosokawa

{"title":"Novel chromosome segment substitution lines derived from japonica cultivar 'Yukihikari' in the genetic background of 'Joiku462' cultivar and identification of quantitative trait loci for heading date and grain quality.","authors":"Kiyoaki Kato, Shinya Munekata, Toshiro Watanabe, Takashi Sato, Yusuke Hosokawa","doi":"10.1270/jsbbs.24058","DOIUrl":"10.1270/jsbbs.24058","url":null,"abstract":"In this study, we mapped quantitative trait loci (QTLs) associated with heading date and grain quality traits in a novel set of 44 chromosome segment substitution lines (CSSLs) derived from closely related rice (Oryza sativa L. ssp. japonica) cultivars 'Yukihikari' (good grain quality) and 'Joiku462' (superior eating and high grain appearance qualities). Days to heading (DTH), apparent amylose content (AAC), protein content (PC), thousand brown-grain weight (TBGW), brown grain length (BGL), brown grain width (BGWI), brown grain thickness (BGT), and the contents of 12 mineral elements (S, P, Mg, Ca, K, Mo, Cu, Zn, Mn, Fe, Sr, and Si) in polished rice were evaluated in 44 CSSLs grown in two different environments. We identified 78 QTLs, including 8, 7, 8, 8, 19, 10, and 10 for DTH, AAC, PC, TBGW, BGL, BGWI, and BGT, respectively, and 2, 1, 3, and 2 for S, Mo, Cu, and Zn contents, respectively. Several QTLs were observed in the same region, forming 17 clusters on chromosomes 1-10. These QTLs can facilitate gene isolation and breeding to develop rice cultivars with optimum heading time and improved grain quality.","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"75 3","pages":"210-223"},"PeriodicalIF":2.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12457784/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145147910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Breeding of Fukumi Fiber, a new six-rowed waxy hull-less barley cultivar containing high levels of β-glucan with a proanthocyanidin-free gene. 含高β-葡聚糖和无原花青素基因的六棱无壳大麦新品种“富麦纤维”的选育。

IF 2.2 4区农林科学

Breeding Science Pub Date : 2025-06-01 Epub Date: 2025-06-21 DOI: 10.1270/jsbbs.24080

Asuka Takahashi, Toji Yoshioka, Takashi Yanagisawa, Takashi Nagamine, Tomohiko Sugita

{"title":"Breeding of Fukumi Fiber, a new six-rowed waxy hull-less barley cultivar containing high levels of β-glucan with a proanthocyanidin-free gene.","authors":"Asuka Takahashi, Toji Yoshioka, Takashi Yanagisawa, Takashi Nagamine, Tomohiko Sugita","doi":"10.1270/jsbbs.24080","DOIUrl":"10.1270/jsbbs.24080","url":null,"abstract":"Fukumi Fiber, a new six-rowed hull-less barley cultivar, has an extremely high β-glucan content; this is the world's first cultivar with two genes (wax and amo1) boosting the content of β-glucan and one gene (ant28.2131) suppressing the browning reaction after cooking, to our knowledge. The β-glucan content of pearled barley is 13.2% in Fukumi Fiber, and is approximately three times higher than that of the standard barley cultivar Ichibanboshi and approximately two times higher than that of the waxy cultivars Daishimochi and Kirari-mochi. Fukumi Fiber has a standard plump grain percentage required for a six-rowed hull-less barley cultivars. The yield is over 10% higher than that of Ichibanboshi. Fukumi Fiber is suitable for cultivation in the plains of central and western Japan and was released in 2018. It can be used for cooked pearled barley and various purposes such as confectionery, noodles, and bread. The spread of this cultivar is expected to lead to a stable supply and the expansion of high-value-added domestic waxy barley.","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"75 3","pages":"236-243"},"PeriodicalIF":2.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12457788/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145147926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of a candidate rice blast resistance gene, Pior4(t), in an introgression line of Oryza rufipogon using CRISPR/Cas9-mediated genome editing. 利用CRISPR/ cas9介导的基因组编辑技术鉴定水稻稻瘟病抗性候选基因Pior4(t)

IF 2.2 4区农林科学

Breeding Science Pub Date : 2025-04-01 Epub Date: 2025-03-26 DOI: 10.1270/jsbbs.24059

Keiko Hayashi, Tomofumi Yoshida, Tarou Suzuki, Masaharu Kuroda, Yuriko Hayano-Saito

{"title":"Identification of a candidate rice blast resistance gene, Pior4(t), in an introgression line of Oryza rufipogon using CRISPR/Cas9-mediated genome editing.","authors":"Keiko Hayashi, Tomofumi Yoshida, Tarou Suzuki, Masaharu Kuroda, Yuriko Hayano-Saito","doi":"10.1270/jsbbs.24059","DOIUrl":"10.1270/jsbbs.24059","url":null,"abstract":"Resistance breeding for rice blast is an economic strategy for protecting rice crops against this disease. Genes with nucleotide-binding site leucine-rich repeat (NBS-LRR) structures are known to contribute to disease resistance. Here, we identified a candidate resistance gene, named Pior4(t), associated with leaf and panicle blasts in an introgression line carrying the chromosome 4 segment of wild rice (Oryza rufipogon Griff.) backcrossed with the cultivar 'Nipponbare' (Oryza sativa L.). Mapping analysis based on leaf blast severity confirmed that Pior4(t) was localized in the 177-kb NBS-LRR cluster region. To identify the Pior4(t) sequence, mutant lines were generated by knocking out a candidate NBS-LRR gene in a homozygous line carrying Pior4(t), M18, using CRISPR/Cas9-mediated genome editing. Leaf blast resistance was lost in the mutant lines lacking the corresponding Os04g0620950 N-terminal sequence of the M18 line. The result suggested that the counterpart NBS-LRR gene in the M18 line is involved in resistance to leaf blast. Pior4(t) showed homology to Pi63 in the resistant cultivar 'Kahei', and an NBS-LRR gene in the resistant cultivar 'Mine-haruka' carrying Pi39(t). These results suggest that the NBS-LRR gene is a candidate gene of Pior4(t) and is present on the long arm of chromosome 4.","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"75 2","pages":"139-146"},"PeriodicalIF":2.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12395198/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144943733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chromosome-level assembly of Cucumis sativus cv. 'Tokiwa' as a reference genome of Japanese cucumber. 黄瓜的染色体水平组装。“Tokiwa”作为日本黄瓜的参考基因组。

IF 2.2 4区农林科学

Breeding Science Pub Date : 2025-04-01 Epub Date: 2025-03-27 DOI: 10.1270/jsbbs.24066

Takashi Seiko, Chiaki Muto, Koichiro Shimomura, Ryoichi Yano, Yoichi Kawazu, Mitsuhiro Sugiyama, Kenji Kato, Norihiko Tomooka, Ken Naito

引用次数: 0