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4区农林科学

Breeding Science Pub Date : 2023-01-01 DOI: 10.1270/jsbbs.73-4cover

引用次数: 0

Identification and characterization of stable QTLs for vascular bundle number at the panicle neck in rice (Oryza sativa L.) 水稻穗部维管束数量稳定qtl的鉴定与表征(Oryza sativa</i&gt)l .)

4区农林科学

Breeding Science Pub Date : 2023-01-01 DOI: 10.1270/jsbbs.23013

Ha Thi Le Nguyen, Shizuka Suetsugu, Yuna Nakamura, Zita Demeter, Shao-Hui Zheng, Daisuke Fujita

{"title":"Identification and characterization of stable QTLs for vascular bundle number at the panicle neck in rice (Oryza sativa L.)","authors":"Ha Thi Le Nguyen, Shizuka Suetsugu, Yuna Nakamura, Zita Demeter, Shao-Hui Zheng, Daisuke Fujita","doi":"10.1270/jsbbs.23013","DOIUrl":"https://doi.org/10.1270/jsbbs.23013","url":null,"abstract":"A large vascular bundle number (VBN) in the panicle neck in rice (Oryza sativa L.) is related to the ability to transport assimilates from stem and leaf to reproductive organs during seed maturation. Several quantitative trait loci (QTLs) for VBN have been identified by using segregating populations derived from a cross be‍tween indica and japonica rice cultivars. However, the detailed location, effect, and interaction of QTLs for VBN were not understood well. Here, to elucidate the genetic basis of VBN, we identified three stable QTLs for VBN—qVBN5, qVBN6 and qVBN11—by using 71 recombinant inbred lines derived from a cross between indica ‘IR24’ and japonica ‘Asominori’. We confirmed their positions and characterized their effects by using chromosome segment substitution lines (CSSLs) with an ‘IR24’ genetic background. qVBN6 had the most substantial effect on VBN, followed by qVBN11 and qVBN5. We developed pyramided lines carrying two QTLs for VBN to estimate their interaction. The combination of qVBN6 and qVBN11 accumulated VBN negatively in the pyramided lines owing to the independent actions of each QTL. The QTLs detected for VBN will enhance our understanding of genetic mechanisms of VBN and can be used in rice breeding.","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135311845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular-based race classification of Pyrenophora tritici-repentis causing tan spot of wheat in Japan Pyrenophora tritrii -repentis在日本造成小麦黑斑病

4区农林科学

Breeding Science Pub Date : 2023-01-01 DOI: 10.1270/jsbbs.23012

Keita Kato, Yusuke Ban, Mikiko Yanaka, Shoya Kitabayashi, Hiroyuki Sekiguchi, Keisuke Tomioka, Miwako Ito

引用次数: 0

Development of pyramided lines carrying brown planthopper resistance genes in the genetic background of Indica Group rice (Oryza sativa L.) variety ‘IR64’ 籼稻(Oryza sativa</i&gt)遗传背景下携带抗褐飞虱基因的金字塔系的发育L.品种“IR64”

4区农林科学

Breeding Science Pub Date : 2023-01-01 DOI: 10.1270/jsbbs.23028

Md. Mostofa Kamal, Cuong Dinh Nguyen, Sachiyo Sanada-Morimura, Shao-Hui Zheng, Daisuke Fujita

{"title":"Development of pyramided lines carrying brown planthopper resistance genes in the genetic background of Indica Group rice (Oryza sativa L.) variety ‘IR64’","authors":"Md. Mostofa Kamal, Cuong Dinh Nguyen, Sachiyo Sanada-Morimura, Shao-Hui Zheng, Daisuke Fujita","doi":"10.1270/jsbbs.23028","DOIUrl":"https://doi.org/10.1270/jsbbs.23028","url":null,"abstract":"The development of resistant rice (Oryza sativa L.) varieties is a key strategy for the eco-friendly control of brown planthopper (BPH: Nilaparvata lugens Stål). However, BPH outbreaks occur frequently owing to the evolution of virulent strains in the field and the rapid breakdown of monogenic resistance to BPH. Therefore, to enhance BPH resistance and gauge the effectiveness of gene pyramiding against strongly virulent BPH, we developed pyramided lines (PYLs) in the genetic background of ‘IR64’ carrying BPH resistance genes. We developed six IR64-PYLs (BPH3 + BPH17, BPH32 + BPH17, BPH32 + BPH20, BPH3 + BPH17-ptb, BPH20 + BPH3, and BPH17-ptb + BPH32) through marker-assisted selection. To assess the resistance of the IR64-PYLs, we conducted antibiosis test, honeydew test, and modified seedbox screening test (MSST) using strongly virulent BPH populations. The level of BPH resistance increased in all six IR64-PYLs compared to ‍both ‘IR64’ and the corresponding NILs in MSST. Among them, IR64-BPH3 + BPH17 and IR64-BPH32 + BPH17 exhibited the highest resistance to BPH. However, the resistance level of most IR64-PYLs was not significantly higher than that of the corresponding NILs in antibiosis test. Thus, these PYLs could serve as a valuable resource for breeding programs aimed at improving resistance to virulent strains of BPH and enhancing their durability.","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"101 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135260963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantitative trait locus (QTL) analysis and fine-mapping for Fusarium oxysporum disease resistance in Raphanus sativus using GRAS-Di technology 尖孢镰刀菌数量性状位点(QTL)分析及精细定位萝卜的抗病性采用grass - di技术

4区农林科学

Breeding Science Pub Date : 2023-01-01 DOI: 10.1270/jsbbs.23032

Chukwunonso Sylvanus Austin Ezeah, Juichi Shimazu, Takahiro Kawanabe, Motoki Shimizu, Shinichi Kawashima, Makoto Kaji, Charles Onyemaechi Ezinma, Md Nuruzzaman, Nami Minato, Eigo Fukai, Keiichi Okazaki

{"title":"Quantitative trait locus (QTL) analysis and fine-mapping for Fusarium oxysporum disease resistance in Raphanus sativus using GRAS-Di technology","authors":"Chukwunonso Sylvanus Austin Ezeah, Juichi Shimazu, Takahiro Kawanabe, Motoki Shimizu, Shinichi Kawashima, Makoto Kaji, Charles Onyemaechi Ezinma, Md Nuruzzaman, Nami Minato, Eigo Fukai, Keiichi Okazaki","doi":"10.1270/jsbbs.23032","DOIUrl":"https://doi.org/10.1270/jsbbs.23032","url":null,"abstract":"Fusarium wilt is a significant disease in radish, but the genetic mechanisms controlling yellows resistance (YR) are not well understood. This study aimed to identify YR-QTLs and to fine-map one of them using F2:3 populations developed from resistant and susceptible radish parents. In this study, two high-density genetic maps each containing shared co-dominant markers and either female or male dominant markers that spanned 988.6 and 1127.5 cM with average marker densities of 1.40 and 1.53 cM, respectively, were generated using Genotyping by Random Amplicon Sequencing-Direct (GRAS-Di) technology. We identified two YR-QTLs on chromosome R2 and R7, and designated the latter as ForRs1 as the major QTL. Fine mapping narrowed down the ForRs1 locus to a 195 kb region. Among the 16 predicted genes in the delimited region, 4 genes including two receptor-like protein and -kinase genes (RLP/RLK) were identified as prime candidates for ForRs1 based on the nucleotide sequence comparisons between the parents and their predicted functions. This study is the first to use a GRAS-Di for genetic map construction of cruciferous crops and fine map the YR-QTL on the R7 chromosome of radish. These findings will provide groundbreaking insights into radish YR breeding and understanding the genetics of YR mechanism.","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"37 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135266907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improvement of preharvest sprouting resistance with MOTHER OF FT AND TFL 1 + mutated ABA 8'-hydroxylase in white-seeded durum wheat. FT母和tfl1 +突变ABA 8′-羟化酶提高白粒硬粒小麦采前抗性

IF 2.4 4区农林科学

Breeding Science Pub Date : 2022-12-01 DOI: 10.1270/jsbbs.22018

Yusuke Ban, Keita Kato, Miwako Ito, Mikiko Yanaka, Kanenori Takata

{"title":"Improvement of preharvest sprouting resistance with MOTHER OF FT AND TFL 1 + mutated ABA 8'-hydroxylase in white-seeded durum wheat.","authors":"Yusuke Ban, Keita Kato, Miwako Ito, Mikiko Yanaka, Kanenori Takata","doi":"10.1270/jsbbs.22018","DOIUrl":"https://doi.org/10.1270/jsbbs.22018","url":null,"abstract":"Improvement of preharvest sprouting (PHS) resistance is an important objective in the breeding of durum wheat (Triticum turgidum ssp. durum (Desf.) Husn.) in Japan, where the harvest timing overlaps with the rainy season. In a previous study, we showed that an R-gene associated with red seed color was the most effective at promoting PHS resistance in durum wheat. However, red-seeded durum wheat is not popular because it discolors pasta. Here, to improve PHS resistance without the R-gene, we introduced a PHS resistance allele of MOTHER OF FT AND TFL 1 (MFT) and a mutated ABA 8'-hydroxylase (ABA8'OH1-A), which is involved in abscisic acid (ABA) catabolism, singly or together into white-seeded durum wheat. The introduction of both genes reliably and stably improved PHS resistance under all tested conditions. Modification of ABA catabolism might be an effective way to improve PHS resistance in durum wheat. Our findings will contribute to improved PHS resistance in breeding for white-seeded durum wheat.","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"72 5","pages":"355-361"},"PeriodicalIF":2.4,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9895805/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9274781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative analyses of Stvb-allelic genes reveal japonica specificity of rice stripe resistance in Oryza sativa. stvb等位基因的比较分析揭示了水稻条纹抗性的粳稻特异性。

IF 2.4 4区农林科学

Breeding Science Pub Date : 2022-12-01 DOI: 10.1270/jsbbs.22027

Keiko Hayashi, Yoshihiro Kawahara, Hideo Maeda, Yuriko Hayano-Saito

{"title":"Comparative analyses of Stvb-allelic genes reveal japonica specificity of rice stripe resistance in Oryza sativa.","authors":"Keiko Hayashi, Yoshihiro Kawahara, Hideo Maeda, Yuriko Hayano-Saito","doi":"10.1270/jsbbs.22027","DOIUrl":"https://doi.org/10.1270/jsbbs.22027","url":null,"abstract":"Rice stripe, a viral disease, causes widespread damage to japonica rice (Oryza sativa ssp. japonica). A rice stripe virus (RSV) bioassay revealed that many indica and japonica upland varieties exhibit resistance, whereas japonica paddy varieties are susceptible. However, the genetic background for this subspecies-dependent resistance is unclear. Herein, we focused on rice stripe resistance genes located at the Stvb locus. Three resistant alleles, Stvb-i (indica), Stvb (japonica upland), and Stvb-o (Oryza officinalis) were compared with the susceptible allele, stvb-j (japonica paddy). The expression of the resistance genes was higher than that of stvb-j. Sequence comparison revealed that the resistant and susceptible alleles had different 5'-end sequences and 61-bp element(s) in the fourth intron. The insertion of an LTR-retrotransposon modified the exon 1 sequence of stvb-j. We then developed four DNA markers based on gene structure information and genotyped resistant and susceptible varieties. The LTR-retrotransposon insertion was detected only in susceptible varieties. Resistant genotypes were primarily found in indica and upland japonica, whereas paddy japonica carried the susceptible genotype. Our results characterize the genetic differences associated with RSV resistance and susceptibility in O. sativa and provide insights on the application of DNA markers in rice stripe disease management.","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"72 5","pages":"333-342"},"PeriodicalIF":2.4,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9895804/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9274784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The induced mutant allele flo4-303 confers floury characteristics on the japonica rice cultivar 'Hoshinoko'. 诱变等位基因flo4-303使粳稻品种星野子具有面粉性状。

IF 2.4 4区农林科学

Breeding Science Pub Date : 2022-12-01 DOI: 10.1270/jsbbs.22059

Shuichi Matsuba, Wakako Maruyama-Funatsuki, Takayuki Umemoto, Hideki Kato, Makoto Kuroki, Narifumi Yokogami, Tomohito Ikegaya, Hiroyuki Shimizu, Norio Iriki

{"title":"The induced mutant allele flo4-303 confers floury characteristics on the japonica rice cultivar 'Hoshinoko'.","authors":"Shuichi Matsuba, Wakako Maruyama-Funatsuki, Takayuki Umemoto, Hideki Kato, Makoto Kuroki, Narifumi Yokogami, Tomohito Ikegaya, Hiroyuki Shimizu, Norio Iriki","doi":"10.1270/jsbbs.22059","DOIUrl":"https://doi.org/10.1270/jsbbs.22059","url":null,"abstract":"Rice flour is useful as a substitute for wheat flour, however, to obtain fine flour, millers need special milling facilities, which increase the cost of milling. To reduce the milling cost, we developed a floury mutant line by irradiating gamma-rays to dry seeds of the japonica cultivar 'Hoshinoyume'. The line was registered as a new cultivar, 'Hoshinoko'. Genetical analysis of the floury trait was conducted using an F2 population derived from a cross between 'Hoshinoko' and 'Corbetti' (a japonica rice cultivar with normal endosperm), which indicated the involvement of a single recessive gene located near the RM163 marker on the long arm of rice chromosome 5, flanking flo4 identified by Kang et al. (2005). Sequence analysis of flo4 showed a two-bp (CA) insertion in the eighth exon of in 'Hoshinoko' compared to that of 'Hoshinoyume', which led to a frameshift mutation. The CAPS-based genotype of flo4 gene completely correlated to the phenotype of endosperm in two populations. This CAPS marker could be helpful for rice breeders to develop new cultivars harboring floury endosperm of the flo4-303 gene.","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"72 5","pages":"383-388"},"PeriodicalIF":2.4,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9895802/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9259078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Allelic variations of Vrn-1 and Ppd-1 genes in Japanese wheat varieties reveal the genotype-environment interaction for heading time. 日本小麦品种中 Vrn-1 和 Ppd-1 基因的等位基因变异揭示了打顶时间的基因型与环境之间的相互作用。

IF 2 4区农林科学

Breeding Science Pub Date : 2022-12-01 Epub Date: 2022-12-06 DOI: 10.1270/jsbbs.22017

Nobuyuki Mizuno, Hitoshi Matsunaka, Mikiko Yanaka, Masaru Nakata, Kazuhiro Nakamura, Akiko Nakamaru, Chikako Kiribuchi-Otobe, Goro Ishikawa, Makiko Chono, Koichi Hatta, Masaya Fujita, Fuminori Kobayashi

{"title":"Allelic variations of Vrn-1 and Ppd-1 genes in Japanese wheat varieties reveal the genotype-environment interaction for heading time.","authors":"Nobuyuki Mizuno, Hitoshi Matsunaka, Mikiko Yanaka, Masaru Nakata, Kazuhiro Nakamura, Akiko Nakamaru, Chikako Kiribuchi-Otobe, Goro Ishikawa, Makiko Chono, Koichi Hatta, Masaya Fujita, Fuminori Kobayashi","doi":"10.1270/jsbbs.22017","DOIUrl":"10.1270/jsbbs.22017","url":null,"abstract":"The timing of heading is largely affected by environmental conditions. In wheat, Vrn-1 and Ppd-1 have been identified as the major genes involved in vernalization requirement and photoperiod sensitivity, respectively. To compare the effects of Vrn-1 and Ppd-1 alleles on heading time under different environments, we genotyped Vrn-1 and Ppd-1 homoeologues and measured the heading time at Morioka, Tsukuba and Chikugo in Japan for two growing seasons. A total of 128 Japanese and six foreign varieties, classified into four populations based on the 519 genome-wide SNPs, were used for analysis. Varieties with the spring alleles (Vrn-D1a or Vrn-D1b) at the Vrn-D1 locus and insensitive allele (Hapl-I) at the Ppd-D1 locus were found in earlier heading varieties. The effects of Vrn-D1 and Ppd-D1 on heading time were stronger than those of the other Vrn-1 and Ppd-1 homoeologues. Analysis of variance revealed that heading time was significantly affected by the genotype-environment interactions. Some Vrn-1 and Ppd-1 alleles conferred earlier or later heading in specific environments, indicating that the effect of both alleles on the timing of heading depends on the environment. Information on Vrn-1 and Ppd-1 alleles, together with heading time in various environments, provide useful information for wheat breeding.","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"72 5","pages":"343-354"},"PeriodicalIF":2.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9895800/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9259079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Two dominant genes in barley (Hordeum vulgare L.) complementarily encode perfect resistance to Japanese soil-borne wheat mosaic virus. 大麦(Hordeum vulgare L.)的两个显性基因互补编码了对日本土传小麦花叶病毒的完全抗性。

IF 2.4 4区农林科学

Breeding Science Pub Date : 2022-12-01 DOI: 10.1270/jsbbs.22046

Kaori Okada, Tsuyoshi Tanaka, Shuichi Fukuoka, Youko Oono, Kohei Mishina, Tetsuo Oikawa, Kazuhiro Sato, Tsuneo Kato, Takao Komatsuda, Kiyoshi Namai

{"title":"Two dominant genes in barley (Hordeum vulgare L.) complementarily encode perfect resistance to Japanese soil-borne wheat mosaic virus.","authors":"Kaori Okada, Tsuyoshi Tanaka, Shuichi Fukuoka, Youko Oono, Kohei Mishina, Tetsuo Oikawa, Kazuhiro Sato, Tsuneo Kato, Takao Komatsuda, Kiyoshi Namai","doi":"10.1270/jsbbs.22046","DOIUrl":"https://doi.org/10.1270/jsbbs.22046","url":null,"abstract":"Japanese soil-borne wheat mosaic virus (Furovirus) is a damaging pathogen of wheat and barley. This virus can survive in the soil for several decades, so the deployment of resistant cultivars represents the only practical control measure. Here, a genetic analysis has identified two regions of the barley genome-one on chromosome 2H and the other on chromosome 3H-as harboring gene(s) encoding resistance to this virus. The joint presence of both loci, termed Jmv1 and Jmv2, made the plants essentially immune, with resistance being dominant over susceptibility at each locus. Phylogenetic analysis showed that the virus is not closely related to the type Furovirus species Soil-borne wheat mosaic virus. There was a difference between the RNA1- and RNA2-based phylogenies of the virus species in Furovirus implying the independent segregation of the virus subgenomes.","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"72 5","pages":"372-382"},"PeriodicalIF":2.4,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9895801/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9274785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2