Breeding Science最新文献

筛选
英文 中文
Wild and cultivated allele effects on rice phenotypic traits in reciprocal backcross populations between Oryza rufipogon and two cultivars, O. sativa Nipponbare and IR36. Oryza rufipogon与两个栽培品种(O. sativa Nipponbare和IR36)互作回交群体中野生和栽培等位基因对水稻表型性状的影响。
IF 2.4 4区 农林科学
Breeding Science Pub Date : 2023-09-01 Epub Date: 2023-09-09 DOI: 10.1270/jsbbs.22095
Phuong Dang Thai Phan, Akinori Nishimura, Chika Yamamoto, Pham Thien Thanh, Toshihiro Niwa, Yaddehige Priya Jayantha Amarasinghe, Ryo Ishikawa, Takashige Ishii
{"title":"Wild and cultivated allele effects on rice phenotypic traits in reciprocal backcross populations between <i>Oryza rufipogon</i> and two cultivars, <i>O. sativa</i> Nipponbare and IR36.","authors":"Phuong Dang Thai Phan, Akinori Nishimura, Chika Yamamoto, Pham Thien Thanh, Toshihiro Niwa, Yaddehige Priya Jayantha Amarasinghe, Ryo Ishikawa, Takashige Ishii","doi":"10.1270/jsbbs.22095","DOIUrl":"10.1270/jsbbs.22095","url":null,"abstract":"<p><p>A total of four populations of reciprocal backcross recombinant inbred lines were produced from a cross between a wild accession of <i>Oryza rufipogon</i> W630 and two major cultivars, <i>O. sativa</i> Japonica Nipponbare and Indica IR36. Using these populations, quantitative trait locus (QTL) analysis for eight morphological traits (culm length, panicle length, days to heading, panicle shape, pericarp color, hull color, seed shattering and seed awning) was carried out, and the putative QTL regions were compared among the populations. The QTLs with strong allele effects were commonly detected for culm length, panicle shape, pericarp color and hull color in all four populations, and their peak locations were close to the major genes of <i>sd1</i>, <i>Spr3</i>, <i>Rc</i> and <i>Bh4</i>, respectively. For panicle length and days to heading, some QTL regions overlapped between two or three populations. In the case of seed shattering and seed awning, strong wild allele effects at major loci were observed only in the populations with cultivated backgrounds. Since the wild and cultivated alleles have never been evaluated in the reciprocal genetic backgrounds, the present results provide new information on gene effects in breeding and domestication studies.</p>","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"1 1","pages":"373-381"},"PeriodicalIF":2.4,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10722096/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66690074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mutagenic effects of nitrogen protoxide and oryzalin on "41 B" and "Fercal" grapevine rootstocks seedlings. 原氧化氮和奥利唑啉对 "41 B "和 "Fercal "葡萄砧木幼苗的诱变作用。
IF 2.4 4区 农林科学
Breeding Science Pub Date : 2023-09-01 Epub Date: 2023-09-09 DOI: 10.1270/jsbbs.23003
Zeki Kara, Osman Doğan
{"title":"Mutagenic effects of nitrogen protoxide and oryzalin on \"41 B\" and \"Fercal\" grapevine rootstocks seedlings.","authors":"Zeki Kara, Osman Doğan","doi":"10.1270/jsbbs.23003","DOIUrl":"10.1270/jsbbs.23003","url":null,"abstract":"<p><p>In this study, the mutagenic effects of different doses and exposure times of oryzalin and Nitrogen Protoxide (N<sub>2</sub>O) were tested for stimulating polyploid on 41 B and Fercal grapevine rootstocks seedlings. Ploidy changes were examined by morphological, cytological, macroscopic, and microscopic methods. Leaf thickness, chlorophyll contents, stomatal sizes, and chloroplast numbers of polyploid seedlings stimulated with mutagens increased but their stomatal densities decreased. Flow cytometry (FC) analyses were performed on 50 samples selected by morphological and microscopic preliminary determinations. In FC analyses, 1 tetraploid seedling and 4 mixoploid seedlings from Fercal offspring and 1 mixoploid seedling from 41 B offspring were verified. The nuclear DNA content of tetraploid and mixoploid seedlings were increased by 2.00 and 1.34-fold, respectively, when compared to their diploid parents. Chromosome counts in root tip samples propagated <i>in vitro</i> from the tetraploid Fercal offspring confirmed a 2-fold increase compared to the diploid parent. In polyploidy induction studies, it was deemed appropriate to use FC analysis and chromosome count together to confirm the ploidy levels of mutants. Oryzalin and N<sub>2</sub>O applications at different doses and exposure times were found to be effective for inducing polyploidy in 41 B and Fercal grapevine rootstocks.</p>","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"1 1","pages":"355-364"},"PeriodicalIF":2.4,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10722092/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66690138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Allergic reaction to heparin gel - Adding agony to blood donor. 肝素凝胶过敏反应--献血者的痛苦。
IF 0.6 4区 农林科学
Breeding Science Pub Date : 2023-07-01 Epub Date: 2022-09-28 DOI: 10.4103/ajts.ajts_131_21
Deepti Sachan, Deepthi Krishna Gundrajukuppam, Kuralarasi Priyadarshini
{"title":"Allergic reaction to heparin gel - Adding agony to blood donor.","authors":"Deepti Sachan, Deepthi Krishna Gundrajukuppam, Kuralarasi Priyadarshini","doi":"10.4103/ajts.ajts_131_21","DOIUrl":"10.4103/ajts.ajts_131_21","url":null,"abstract":"","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"14 1","pages":"307-308"},"PeriodicalIF":0.6,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10807521/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88836257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Near-isogenic lines for resistance to brown planthopper with the genetic background of Indica Group elite rice (Oryza sativa L.) variety 'IR64'. 以籼稻(Oryza sativa L.)优良品种‘IR64’为遗传背景的抗褐飞虱近等基因系。
IF 2.4 4区 农林科学
Breeding Science Pub Date : 2023-06-01 Epub Date: 2023-06-15 DOI: 10.1270/jsbbs.22093
Md Mostofa Kamal, Cuong Dinh Nguyen, Sachiyo Sanada-Morimura, Shao-Hui Zheng, Daisuke Fujita
{"title":"Near-isogenic lines for resistance to brown planthopper with the genetic background of Indica Group elite rice (<i>Oryza sativa</i> L.) variety 'IR64'.","authors":"Md Mostofa Kamal,&nbsp;Cuong Dinh Nguyen,&nbsp;Sachiyo Sanada-Morimura,&nbsp;Shao-Hui Zheng,&nbsp;Daisuke Fujita","doi":"10.1270/jsbbs.22093","DOIUrl":"10.1270/jsbbs.22093","url":null,"abstract":"<p><p>The brown planthopper (BPH), <i>Nilaparvata lugens</i> Stål, is an insect pest that severely damages rice (<i>Oryza sativa</i> L.) in Asia, causing huge yield loss. Use of resistant variety is a cost-effective and eco-friendly strategy for maintaining BPH populations below the economic injury level. However, current BPH populations have been changed to virulence against resistant varieties. In this study, to estimate effective combinations among eight BPH resistance genes (<i>BPH32</i>, <i>BPH17-ptb</i>, <i>BPH20</i>, <i>BPH17</i>, <i>BPH3</i>, <i>BPH25</i>, <i>BPH26</i> and <i>qBPH6</i>), eight near-isogenic lines with the genetic background of an Indica Group rice variety 'IR64' (IR64-NIL) were developed using marker-assisted selection. The genome recoveries of these NILs ranged from 89.3% to 98.8% and agronomic traits of them were similar to those of 'IR64'. In modified seed box screening test, resistance level of IR64-NILs was higher than that of 'IR64'. In antibiosis test, high adult mortalities of BPH (from 56.0% to 97.0%) were observed among NILs, in comparison with that of 'IR64'. Among IR64-NILs, the line carrying <i>BPH17</i> showed the highest resistance level at all tests. Thus, these IR64-NILs with multiple BPH resistance genes could be valuable breeding lines for enhancing resistance levels by gene pyramiding and multiline variety.</p>","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"73 3","pages":"278-289"},"PeriodicalIF":2.4,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10570883/pdf/73_278.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41232444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Allyl isothiocyanate and 6-(methylsulfinyl) hexyl isothiocyanate contents vary among wild and cultivated wasabi (Eutrema japonium). 异硫氰酸烯丙酯和6-(甲基亚磺酰基)己基异硫氰酸酯的含量在野生和栽培的山葵(Eutrema japonium)中不同。
IF 2.4 4区 农林科学
Breeding Science Pub Date : 2023-06-01 Epub Date: 2023-06-15 DOI: 10.1270/jsbbs.22080
Kyoko Yamane, Tomoe Yamada-Kato, Natsuko Haga, Kaori Ishida, Seiji Murayama, Keiko Kobayashi, Isao Okunishi
{"title":"Allyl isothiocyanate and 6-(methylsulfinyl) hexyl isothiocyanate contents vary among wild and cultivated wasabi (<i>Eutrema japonium</i>).","authors":"Kyoko Yamane,&nbsp;Tomoe Yamada-Kato,&nbsp;Natsuko Haga,&nbsp;Kaori Ishida,&nbsp;Seiji Murayama,&nbsp;Keiko Kobayashi,&nbsp;Isao Okunishi","doi":"10.1270/jsbbs.22080","DOIUrl":"10.1270/jsbbs.22080","url":null,"abstract":"<p><p>Wasabi (Japanese horseradish, <i>Eutrema japonicum</i>) is the only cultivated species in the genus <i>Eutrema</i> with functional components that provide a strong pungent flavor. To evaluate genetic resources for wasabi breeding, we surveyed variations in the two most abundant isothiocyanate (ITC) components in wasabi, allyl isothiocyanate (AITC) and 6-methylsulfinyl (hexyl) isothiocyanate (6-MSITC, hexaraphane). We also examined the phylogenetic relationships among 36 accessions of wild and cultivated wasabi in Japan using chloroplast DNA analysis. Our results showed that (i) the 6-MSITC content in currently cultivated wasabi accessions was significantly higher than in escaped cultivars, whereas the AITC content was not significantly different. (ii) Additionally, the 6-MSITC content in cultivated wasabi was significantly lower in the spring than during other seasons. This result suggested that the 6-MSITC content responds to environmental conditions. (iii) The phylogenetic position and the 6-MSITC content of accessions from Rebun, Hokkaido Prefecture had different profiles compared with those from southern Honshu, Japan, indicating heterogeneity of the Rebun populations from other Japanese wasabi accessions. (iv) The total content of AITC and 6-MSITC in cultivated wasabi was significantly higher than that of wild wasabi. In conclusion, old cultivars or landraces of wasabi, \"zairai\", are the most suitable candidates for immediate use as genetic resources.</p>","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"73 3","pages":"237-245"},"PeriodicalIF":2.4,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10570882/pdf/73_237.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41232435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Elucidation of genetic variation and population structure of melon genetic resources in the NARO Genebank, and construction of the World Melon Core Collection. NARO基因库甜瓜遗传资源的遗传变异和群体结构的阐明,以及世界甜瓜核心种质的构建。
IF 2.4 4区 农林科学
Breeding Science Pub Date : 2023-06-01 Epub Date: 2023-06-15 DOI: 10.1270/jsbbs.22071
Gentaro Shigita, Tran Phuong Dung, Mst Naznin Pervin, Thanh-Thuy Duong, Odirich Nnennaya Imoh, Yuki Monden, Hidetaka Nishida, Katsunori Tanaka, Mitsuhiro Sugiyama, Yoichi Kawazu, Norihiko Tomooka, Kenji Kato
{"title":"Elucidation of genetic variation and population structure of melon genetic resources in the NARO Genebank, and construction of the World Melon Core Collection.","authors":"Gentaro Shigita,&nbsp;Tran Phuong Dung,&nbsp;Mst Naznin Pervin,&nbsp;Thanh-Thuy Duong,&nbsp;Odirich Nnennaya Imoh,&nbsp;Yuki Monden,&nbsp;Hidetaka Nishida,&nbsp;Katsunori Tanaka,&nbsp;Mitsuhiro Sugiyama,&nbsp;Yoichi Kawazu,&nbsp;Norihiko Tomooka,&nbsp;Kenji Kato","doi":"10.1270/jsbbs.22071","DOIUrl":"10.1270/jsbbs.22071","url":null,"abstract":"<p><p>Numerous genetic resources of major crops have been introduced from around the world and deposited in Japanese National Agriculture and Food Research Organization (NARO) Genebank. Understanding their genetic variation and selecting a representative subset (\"core collection\") are essential for optimal management and efficient use of genetic resources. In this study, we conducted genotyping-by-sequencing (GBS) to characterize the genetic relationships and population structure in 755 accessions of melon genetic resources. The GBS identified 39,324 single-nucleotide polymorphisms (SNPs) that are distributed throughout the melon genome with high density (one SNP/10.6 kb). The phylogenetic relationships and population structure inferred using this SNP dataset are highly associated with the cytoplasm type and geographical origin. Our results strongly support the recent hypothesis that cultivated melon was established in Africa and India through multiple independent domestication events. Finally, we constructed a World Melon Core Collection that covers at least 82% of the genetic diversity and has a wide range of geographical origins and fruit morphology. The genome-wide SNP dataset, phylogenetic relationships, population structure, and the core collection provided in this study should largely contribute to genetic research, breeding, and genetic resource preservation in melon.</p>","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"73 3","pages":"269-277"},"PeriodicalIF":2.4,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10570884/pdf/73_269.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41232441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cloning and functional study of GmRPI2, which is the critical gene of photosynthesis in soybean. 大豆光合作用关键基因GmRPI2的克隆及功能研究。
IF 2.4 4区 农林科学
Breeding Science Pub Date : 2023-06-01 Epub Date: 2023-06-27 DOI: 10.1270/jsbbs.23002
Yu Wei Sun, Xin Yu Wang, Lu Liu, Qi Zhang, Yong Jing Xi, Pi Wu Wang
{"title":"Cloning and functional study of <i>GmRPI2</i>, which is the critical gene of photosynthesis in soybean.","authors":"Yu Wei Sun,&nbsp;Xin Yu Wang,&nbsp;Lu Liu,&nbsp;Qi Zhang,&nbsp;Yong Jing Xi,&nbsp;Pi Wu Wang","doi":"10.1270/jsbbs.23002","DOIUrl":"10.1270/jsbbs.23002","url":null,"abstract":"<p><p>Light provides energy for photosynthesis and is also an important environmental signal that regulates plant growth and development. Ribose-5-phosphate isomerase plays a crucial role in photosynthesis. However, ribose-5-phosphate isomerase has yet to be studied in soybean photosynthesis. To understand the biological function of <i>GmRPI2</i>, in this study, <i>GmRPI2</i> was cloned, plant overexpression vectors and gene editing vectors were successfully constructed, and transformed into recipient soybean JN74 using the Agrobacterium-mediated method. Using qRT-PCR, we analyzed that <i>GmRPI2</i> gene expression was highest in leaves, second highest in roots, and lowest in stems. Promoter analysis revealed the presence of multiple cis-acting elements related to light response in the promoter region of <i>GmRPI2</i>. Compared with the control soybean plants, the net photosynthetic rate and transpiration rate of the overexpression lines were higher than those of the control and gene editing lines, while the intercellular CO<sub>2</sub> concentration was significantly lower than that of the control and gene editing lines; the total chlorophyll, chlorophyll a, chlorophyll b contents and soluble sugar contents of the overexpression plants were significantly higher than those of the recipient and editing plants, indicating that the <i>GmRPI2</i> gene can increase The <i>GmRPI2</i> gene can increase the photosynthetic capacity of soybean plants, providing a theoretical basis and genetic resources for improving soybean yield by regulating photosynthetic efficiency.</p>","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"73 3","pages":"290-299"},"PeriodicalIF":2.4,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10570876/pdf/73_290.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41232437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development of 'Poroshiri', a chip processing potato variety with golden cyst nematode and common scab resistance. 具有金囊线虫和常见赤霉病抗性的马铃薯芯片加工品种“Poroshiri”的开发。
IF 2.4 4区 农林科学
Breeding Science Pub Date : 2023-06-01 Epub Date: 2023-06-15 DOI: 10.1270/jsbbs.22084
Mutsuo Tsuyama, Shogo Ogawa, Keiichi Ogawa, Toshiya Igarashi, Emiko Koizumi
{"title":"Development of 'Poroshiri', a chip processing potato variety with golden cyst nematode and common scab resistance.","authors":"Mutsuo Tsuyama,&nbsp;Shogo Ogawa,&nbsp;Keiichi Ogawa,&nbsp;Toshiya Igarashi,&nbsp;Emiko Koizumi","doi":"10.1270/jsbbs.22084","DOIUrl":"10.1270/jsbbs.22084","url":null,"abstract":"<p><p>Golden cyst nematodes have threatened the cultivation of 'Toyoshiro', a major potato variety used for chip processing in Japan. Common scab is a soilborne disease that occurs in potato fields worldwide. To solve these problems, we crossed two US varieties and selected a clone that showed a slightly higher marketable yield and a significantly higher yield rate, compared with 'Toyoshiro', and had good chip processing quality, extreme resistance to cyst nematodes, and moderately high resistance to common scab. This clone was named 'Poroshiri'; it is the first variety released from the Calbee Potato breeding program.</p>","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"73 3","pages":"343-348"},"PeriodicalIF":2.4,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10570885/pdf/73_343.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41232440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Chromatographic printed array strip (C-PAS) method for cultivar-specific identification of sweetpotato cultivars 'Beniharuka' and 'Fukumurasaki'. 用于甘薯品种“Beniharuka”和“Fukumurasaki”品种特异性鉴定的色谱印刷阵列条(C-PAS)法。
IF 2.4 4区 农林科学
Breeding Science Pub Date : 2023-06-01 Epub Date: 2023-06-28 DOI: 10.1270/jsbbs.22101
Yuki Monden, Maho Kakigi, Emdadul Haque, Tomoyuki Takeuchi, Kazuto Takasaki, Masaru Tanaka
{"title":"Chromatographic printed array strip (C-PAS) method for cultivar-specific identification of sweetpotato cultivars 'Beniharuka' and 'Fukumurasaki'.","authors":"Yuki Monden,&nbsp;Maho Kakigi,&nbsp;Emdadul Haque,&nbsp;Tomoyuki Takeuchi,&nbsp;Kazuto Takasaki,&nbsp;Masaru Tanaka","doi":"10.1270/jsbbs.22101","DOIUrl":"10.1270/jsbbs.22101","url":null,"abstract":"<p><p>Sweetpotato (<i>Ipomoea batatas</i>) cultivars grown in Japan are highly valued for their excellent sweetness, high quality, and good texture. The export volume of sweetpotato from Japan has been rising rapidly, with a 10-fold increase on a weight basis over the last 10 years. However, since sweetpotato is propagated vegetatively from storage roots, it is easy to cultivate and propagate this crop, prompting concerns that Japanese sweetpotato cultivars/lines are being exported overseas, cultivated without permission, or reimported. Therefore, a rapid and accurate cultivar identification methodology is needed. In this study, we comprehensively analyzed the insertion sites of <i>Cl8</i> retrotransposon to develop a cultivar identification technique for the Japanese cultivars 'Beniharuka' and 'Fukumurasaki'. These two cultivars were successfully distinguished from other cultivars using a minimum of two marker sets. Using the chromatographic printed array strip (C-PAS) method for DNA signal detection, 'Beniharuka' and 'Fukumurasaki' can be precisely identified using a single strip of chromatographic paper based on multiplex DNA signals derived from the amplicons of the <i>Cl8</i> insertion sites. Since this method can detect DNA signals in only ~15 minutes, we expect that our method will facilitate rapid, reliable, and convenient cultivar discrimination for on-site inspection of sweetpotato.</p>","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"73 3","pages":"313-321"},"PeriodicalIF":2.4,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10570877/pdf/73_313.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41232436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mutant Tof11 alleles are highly accumulated in early planting-adaptable Japanese summer type soybeans. 突变体Tof11等位基因在早期种植的适应性强的日本夏型大豆中高度积累。
IF 2.4 4区 农林科学
Breeding Science Pub Date : 2023-06-01 Epub Date: 2023-06-28 DOI: 10.1270/jsbbs.22098
Kunihiko Komatsu, Takashi Sayama, Ken-Ichiro Yamashita, Yoshitake Takada
{"title":"Mutant <i>Tof11</i> alleles are highly accumulated in early planting-adaptable Japanese summer type soybeans.","authors":"Kunihiko Komatsu,&nbsp;Takashi Sayama,&nbsp;Ken-Ichiro Yamashita,&nbsp;Yoshitake Takada","doi":"10.1270/jsbbs.22098","DOIUrl":"10.1270/jsbbs.22098","url":null,"abstract":"<p><p>To avoid crop failure because of climate change, soybean (<i>Glycine max</i> (L.) Merrill) cultivars adaptable to early planting are required in western Japan. Because current Japanese cultivars may not be adaptable, genetic resources with high early-planting adaptability, and their genetic information must be developed. In the present study, summer type (ST) soybeans developed for early planting were used as plant materials. We examined their phenological characteristics and short reproductive period as an indicator of early planting adaptability and performed genetic studies. Biparental quantitative trait loci (QTL) analysis of a representative ST cultivar revealed a principal QTL for the reproductive period duration on chromosome 11. The results of resequencing analysis suggested that circadian clock-related <i>Tof11</i> (soybean orthologue of <i>PRR3</i>) is a candidate QTL. Additionally, all 25 early planting-adaptable germplasms evaluated in this study possessed mutant alleles in <i>Tof11</i>, whereas 15 conventional cultivars only had wild-type alleles. These results suggest that mutant alleles in <i>Tof11</i> are important genetic factors in the high adaptability to early planting of these soybeans, and thus, these alleles were acquired and accumulated in the ST soybean population.</p>","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"73 3","pages":"322-331"},"PeriodicalIF":2.4,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10570879/pdf/73_322.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41232443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信