Breeding Science最新文献_第7页

Chromatographic printed array strip (C-PAS) method for cultivar-specific identification of sweetpotato cultivars 'Beniharuka' and 'Fukumurasaki'. 用于甘薯品种“Beniharuka”和“Fukumurasaki”品种特异性鉴定的色谱印刷阵列条（C-PAS）法。

IF 2.4 4区农林科学

Breeding Science Pub Date : 2023-06-01 Epub Date: 2023-06-28 DOI: 10.1270/jsbbs.22101

Yuki Monden, Maho Kakigi, Emdadul Haque, Tomoyuki Takeuchi, Kazuto Takasaki, Masaru Tanaka

{"title":"Chromatographic printed array strip (C-PAS) method for cultivar-specific identification of sweetpotato cultivars 'Beniharuka' and 'Fukumurasaki'.","authors":"Yuki Monden, Maho Kakigi, Emdadul Haque, Tomoyuki Takeuchi, Kazuto Takasaki, Masaru Tanaka","doi":"10.1270/jsbbs.22101","DOIUrl":"10.1270/jsbbs.22101","url":null,"abstract":"Sweetpotato (Ipomoea batatas) cultivars grown in Japan are highly valued for their excellent sweetness, high quality, and good texture. The export volume of sweetpotato from Japan has been rising rapidly, with a 10-fold increase on a weight basis over the last 10 years. However, since sweetpotato is propagated vegetatively from storage roots, it is easy to cultivate and propagate this crop, prompting concerns that Japanese sweetpotato cultivars/lines are being exported overseas, cultivated without permission, or reimported. Therefore, a rapid and accurate cultivar identification methodology is needed. In this study, we comprehensively analyzed the insertion sites of Cl8 retrotransposon to develop a cultivar identification technique for the Japanese cultivars 'Beniharuka' and 'Fukumurasaki'. These two cultivars were successfully distinguished from other cultivars using a minimum of two marker sets. Using the chromatographic printed array strip (C-PAS) method for DNA signal detection, 'Beniharuka' and 'Fukumurasaki' can be precisely identified using a single strip of chromatographic paper based on multiplex DNA signals derived from the amplicons of the Cl8 insertion sites. Since this method can detect DNA signals in only ~15 minutes, we expect that our method will facilitate rapid, reliable, and convenient cultivar discrimination for on-site inspection of sweetpotato.","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"73 3","pages":"313-321"},"PeriodicalIF":2.4,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10570877/pdf/73_313.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41232436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mutant Tof11 alleles are highly accumulated in early planting-adaptable Japanese summer type soybeans. 突变体Tof11等位基因在早期种植的适应性强的日本夏型大豆中高度积累。

IF 2.4 4区农林科学

Breeding Science Pub Date : 2023-06-01 Epub Date: 2023-06-28 DOI: 10.1270/jsbbs.22098

Kunihiko Komatsu, Takashi Sayama, Ken-Ichiro Yamashita, Yoshitake Takada

{"title":"Mutant Tof11 alleles are highly accumulated in early planting-adaptable Japanese summer type soybeans.","authors":"Kunihiko Komatsu, Takashi Sayama, Ken-Ichiro Yamashita, Yoshitake Takada","doi":"10.1270/jsbbs.22098","DOIUrl":"10.1270/jsbbs.22098","url":null,"abstract":"To avoid crop failure because of climate change, soybean (Glycine max (L.) Merrill) cultivars adaptable to early planting are required in western Japan. Because current Japanese cultivars may not be adaptable, genetic resources with high early-planting adaptability, and their genetic information must be developed. In the present study, summer type (ST) soybeans developed for early planting were used as plant materials. We examined their phenological characteristics and short reproductive period as an indicator of early planting adaptability and performed genetic studies. Biparental quantitative trait loci (QTL) analysis of a representative ST cultivar revealed a principal QTL for the reproductive period duration on chromosome 11. The results of resequencing analysis suggested that circadian clock-related Tof11 (soybean orthologue of PRR3) is a candidate QTL. Additionally, all 25 early planting-adaptable germplasms evaluated in this study possessed mutant alleles in Tof11, whereas 15 conventional cultivars only had wild-type alleles. These results suggest that mutant alleles in Tof11 are important genetic factors in the high adaptability to early planting of these soybeans, and thus, these alleles were acquired and accumulated in the ST soybean population.","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"73 3","pages":"322-331"},"PeriodicalIF":2.4,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10570879/pdf/73_322.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41232443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Studies of potato resistance to Globodera rostochiensis revealed novel alleles for 57R marker. 对马铃薯抗玫瑰球藻的研究揭示了57R标记的新等位基因。

IF 2.4 4区农林科学

Breeding Science Pub Date : 2023-06-01 Epub Date: 2023-07-07 DOI: 10.1270/jsbbs.22094

Anna Ivanova-Pozdejeva, Liina Jakobson, Kai Ilves, Agnes Kivistik, Liina Kann, Jekaterina Aida, Liisa Kübarsepp, Terje Tähtjärv, Kristiina Laanemets

{"title":"Studies of potato resistance to Globodera rostochiensis revealed novel alleles for 57R marker.","authors":"Anna Ivanova-Pozdejeva, Liina Jakobson, Kai Ilves, Agnes Kivistik, Liina Kann, Jekaterina Aida, Liisa Kübarsepp, Terje Tähtjärv, Kristiina Laanemets","doi":"10.1270/jsbbs.22094","DOIUrl":"10.1270/jsbbs.22094","url":null,"abstract":"Globodera rostochiensis resistance has been an important trait in potato (Solanum tuberosum) breeding for decades. Our aim was to complement phenotypic testing with genetic marker analysis. We analysed the results of G. rostochiensis resistance greenhouse testing in 4601 tubers of 2918 breeding clones from 11 years. Applicability of H1 gene markers TG689 and 57R was compared. We implemented the latter with the positive predictive value of 99.1% and negative predictive value of 60.0% into the breeding scheme. The 57R marker alleles of 22 Estonian cultivars and 470 breeding clones were determined. Two unique 57R alleles, 57R-887 and 57R-1155, were found in Estonian cultivar 'Anti'. The 887 bp allele has two deletions (14 bp and 490 bp) accompanied by several other indels and SNPs within the 57R marker region. The 1155 bp allele has three deletions (7 bp, 20 bp and 210 bp) accompanied by several other indels and SNPs within the same region. Partial resistance to G. rostochiensis in 'Anti' suggests that the newly described alleles could affect the H1-mediated resistance directly or indirectly.","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"73 3","pages":"300-312"},"PeriodicalIF":2.4,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10570887/pdf/73_300.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41232445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of 12 sets of chromosome segment substitution lines that enhance allele mining in Asian cultivated rice. 在亚洲栽培水稻中开发12套染色体片段替代系，以增强等位基因挖掘。

IF 2.4 4区农林科学

Breeding Science Pub Date : 2023-06-01 Epub Date: 2023-06-27 DOI: 10.1270/jsbbs.23006

Kazufumi Nagata, Yasunori Nonoue, Kazuki Matsubara, Ritsuko Mizobuchi, Nozomi Ono, Taeko Shibaya, Kaworu Ebana, Eri Ogiso-Tanaka, Takanari Tanabata, Kazuhiko Sugimoto, Fumio Taguchi-Shiobara, Jun-Ichi Yonemaru, Yusaku Uga, Atsunori Fukuda, Tadamasa Ueda, Shin-Ichi Yamamoto, Utako Yamanouchi, Toshiyuki Takai, Takashi Ikka, Katsuhiko Kondo, Tomoki Hoshino, Eiji Yamamoto, Shunsuke Adachi, Jian Sun, Noriyuki Kuya, Yuka Kitomi, Ken Iijima, Hideki Nagasaki, Ayahiko Shomura, Tatsumi Mizubayashi, Noriyuki Kitazawa, Kiyosumi Hori, Tsuyu Ando, Toshio Yamamoto, Shuichi Fukuoka, Masahiro Yano

{"title":"Development of 12 sets of chromosome segment substitution lines that enhance allele mining in Asian cultivated rice.","authors":"Kazufumi Nagata, Yasunori Nonoue, Kazuki Matsubara, Ritsuko Mizobuchi, Nozomi Ono, Taeko Shibaya, Kaworu Ebana, Eri Ogiso-Tanaka, Takanari Tanabata, Kazuhiko Sugimoto, Fumio Taguchi-Shiobara, Jun-Ichi Yonemaru, Yusaku Uga, Atsunori Fukuda, Tadamasa Ueda, Shin-Ichi Yamamoto, Utako Yamanouchi, Toshiyuki Takai, Takashi Ikka, Katsuhiko Kondo, Tomoki Hoshino, Eiji Yamamoto, Shunsuke Adachi, Jian Sun, Noriyuki Kuya, Yuka Kitomi, Ken Iijima, Hideki Nagasaki, Ayahiko Shomura, Tatsumi Mizubayashi, Noriyuki Kitazawa, Kiyosumi Hori, Tsuyu Ando, Toshio Yamamoto, Shuichi Fukuoka, Masahiro Yano","doi":"10.1270/jsbbs.23006","DOIUrl":"10.1270/jsbbs.23006","url":null,"abstract":"Many agronomic traits that are important in rice breeding are controlled by multiple genes. The extensive time and effort devoted so far to identifying and selecting such genes are still not enough to target multiple agronomic traits in practical breeding in Japan because of a lack of suitable plant materials in which to efficiently detect and validate beneficial alleles from diverse genetic resources. To facilitate the comprehensive analysis of genetic variation in agronomic traits among Asian cultivated rice, we developed 12 sets of chromosome segment substitution lines (CSSLs) with the japonica background, 11 of them in the same genetic background, using donors representing the genetic diversity of Asian cultivated rice. Using these materials, we overviewed the chromosomal locations of 1079 putative QTLs for seven agronomic traits and their allelic distribution in Asian cultivated rice through multiple linear regression analysis. The CSSLs will allow the effects of putative QTLs in the highly homogeneous japonica background to be validated.","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"73 3","pages":"332-342"},"PeriodicalIF":2.4,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10570878/pdf/73_332.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41232438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-wide association study of ear tip barrenness in waxy maize. 糯玉米穗尖不育的全基因组关联研究。

IF 2.4 4区农林科学

Breeding Science Pub Date : 2023-06-01 Epub Date: 2023-06-27 DOI: 10.1270/jsbbs.22056

Xudong Song, Guangfei Zhou, Zhenliang Zhang, Huiming Zhang, Lin Xue, Hui Wang, Mingliang Shi, Huhua Lu, Yuxiang Mao, Guoqing Chen, Xiaolan Huang, Hongjian Zheng, Derong Hao

{"title":"Genome-wide association study of ear tip barrenness in waxy maize.","authors":"Xudong Song, Guangfei Zhou, Zhenliang Zhang, Huiming Zhang, Lin Xue, Hui Wang, Mingliang Shi, Huhua Lu, Yuxiang Mao, Guoqing Chen, Xiaolan Huang, Hongjian Zheng, Derong Hao","doi":"10.1270/jsbbs.22056","DOIUrl":"10.1270/jsbbs.22056","url":null,"abstract":"Ear tip-barrenness (ETB), which results from aborted kernels or infertile florets at the ear tip, is an undesirable factor affecting the yield and quality of waxy maize. To uncover the genetic basis of ETB, a genome-wide association study (GWAS) was conducted using the genotype with 27,354 SNPs and phenotype with three environments. Five SNPs that distributed on chromosomes 1, 3 and 6, were identified to be significantly associated with ETB based on the threshold of false discovery rate (FDR) at 0.05. Among these significant loci, three SNPs were clustered together and colocalized with genomic regions previously reported. The average length of ETB decreased almost linearly from the inbred lines containing no favorable alleles across the three loci (1.75 cm) to those with one (1.18 cm), two (0.94 cm) and three (0.65 cm) favorable alleles. Moreover, three important genes, Zm00001d030028, Zm00001d041510 and Zm00001d038676 were predicted for three significant QTLs, respectively. These results promote the understanding genetic basis for ETB and will be useful for breeding waxy maize varieties with high-quality and high-yield.","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"73 3","pages":"261-268"},"PeriodicalIF":2.4,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10570881/pdf/73_261.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41232442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Whole-genome sequence analysis of mutations in rice plants regenerated from zygotes, mature embryos, and immature embryos. 从受精卵、成熟胚胎和未成熟胚胎再生的水稻植株突变的全基因组序列分析。

IF 2.4 4区农林科学

Breeding Science Pub Date : 2023-06-01 Epub Date: 2023-06-15 DOI: 10.1270/jsbbs.22100

Masako Ichikawa, Norio Kato, Erika Toda, Masakazu Kashihara, Yuji Ishida, Yukoh Hiei, Sachiko N Isobe, Kenta Shirasawa, Hideki Hirakawa, Takashi Okamoto, Toshihiko Komari

{"title":"Whole-genome sequence analysis of mutations in rice plants regenerated from zygotes, mature embryos, and immature embryos.","authors":"Masako Ichikawa, Norio Kato, Erika Toda, Masakazu Kashihara, Yuji Ishida, Yukoh Hiei, Sachiko N Isobe, Kenta Shirasawa, Hideki Hirakawa, Takashi Okamoto, Toshihiko Komari","doi":"10.1270/jsbbs.22100","DOIUrl":"10.1270/jsbbs.22100","url":null,"abstract":"Somaclonal variation was studied by whole-genome sequencing in rice plants (Oryza sativa L., 'Nipponbare') regenerated from the zygotes, mature embryos, and immature embryos of a single mother plant. The mother plant and its seed-propagated progeny were also sequenced. A total of 338 variants of the mother plant sequence were detected in the progeny, and mean values ranged from 9.0 of the seed-propagated plants to 37.4 of regenerants from mature embryos. The natural mutation rate of 1.2 × 10-8 calculated using the variants in the seed-propagated plants was consistent with the values reported previously. The ratio of single nucleotide variants (SNVs) among the variants in the seed-propagated plants was 91.1%, which is higher than 56.1% previously reported, and not significantly different from those in the regenerants. Overall, the ratio of transitions to transversions of SNVs was lower in the regenerants as shown previously. Plants regenerated from mature embryos had significantly more variants than different progeny types. Therefore, using zygotes and immature embryos can reduce somaclonal variation during the genetic manipulation of rice.","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"73 3","pages":"349-353"},"PeriodicalIF":2.4,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10570880/pdf/73_349.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41232446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a multicriteria decision-making model for evaluating hybrid offspring in the sweetpotato (Ipomoea batatas L.) breeding process. 在甘薯（Ipomoea batatas L.）育种过程中评估杂交后代的多准则决策模型的开发。

IF 2.4 4区农林科学

Breeding Science Pub Date : 2023-06-01 Epub Date: 2023-06-27 DOI: 10.1270/jsbbs.22096

Wei Xiang, Kailong Li, Fang Dong, Ya Zhang, Qiang Zeng, Ling Jiang, Daowei Zhang, Yanlan Huang, Liang Xiao, Zhuo Zhang, Chaofan Zhang

{"title":"Development of a multicriteria decision-making model for evaluating hybrid offspring in the sweetpotato (Ipomoea batatas L.) breeding process.","authors":"Wei Xiang, Kailong Li, Fang Dong, Ya Zhang, Qiang Zeng, Ling Jiang, Daowei Zhang, Yanlan Huang, Liang Xiao, Zhuo Zhang, Chaofan Zhang","doi":"10.1270/jsbbs.22096","DOIUrl":"10.1270/jsbbs.22096","url":null,"abstract":"Sweetpotato variety breeding is always a long process. Screening of hybrid offspring is dominated by empirical judgment in this process. Data analysis and decision fatigue have been troubling breeders. In recent years, the low-efficiency screening mode has been unable to meet the requirements of sweetpotato germplasm innovation. Therefore, it is necessary to construct a high-efficiency method that can screen germplasms for different usages, for mining elite genotypes, and to create dedicated sweetpotato varieties. In this article, the multicriteria decision-making (MCDM) model was constructed based on six agronomic traits, including fresh root yield, vine length, vine diameter, branch number, root number and the spatial distribution of storage roots, and five quality traits, including dry matter content, marketable root yield, uniformity of roots, starch content and the edible quality score. Among these, the edible quality score was calculated by using fuzzy comprehensive evaluation to integrate the sensory scores of color, odor, sweetness, stickiness and fibrous taste. The MCDM model was compared with the traditional screening method via an evaluation in 25 sweetpotato materials. The interference of subjective factors on the evaluation results was significantly reduced. The MCDM model is more overall, more accurate and faster than the traditional screening method in the selection of elite sweetpotato materials. It could be programmed to serve the breeders in combination with the traditional screening method.","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"73 3","pages":"246-260"},"PeriodicalIF":2.4,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10570886/pdf/73_246.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41232439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of male sterile lines of CMS chilies (Capsicum annuum L.) from F₁ hybrids. CMS辣椒(Capsicum annuum L.)杂种雄性不育系的发育。

IF 2.4 4区农林科学

Breeding Science Pub Date : 2023-04-01 DOI: 10.1270/jsbbs.22042

Maneechat Nikornpun, Kridsada Sukwiwat, Kittisak Wongsing, Jutamas Kumchai

{"title":"Development of male sterile lines of CMS chilies (Capsicum annuum L.) from F1 hybrids.","authors":"Maneechat Nikornpun, Kridsada Sukwiwat, Kittisak Wongsing, Jutamas Kumchai","doi":"10.1270/jsbbs.22042","DOIUrl":"https://doi.org/10.1270/jsbbs.22042","url":null,"abstract":"Selfing and crossing methods were used to develop the cytoplasmic male sterility (CMS) lines from 2 elite F1 hybrids of CMS hot chilies. The pungency of the CMS lines was improved by backcrossing with the B cultivar. The first and second backcrossed progenies of the CMS lines showed significantly higher capsaicin contents than the F1 hybrids. One good female line K16 × BBC2 (K16), was selected and backcrossed with 3 good maintainer cultivars, C5, C9 and C0. Some incomplete male sterility of pollens was demonstrated in the F1 hybrids and the 1st backcrossed progenies while the partial sterility disappeared by the stage of the second and third generations of backcrossing. When K16 and P32 were crossed with restorers, fruit yields and yield components of certain F1 hybrids, parental lines and commercial varieties were significantly different. Heterosis of yield and yield components of the F1 hybrid chilies was significant. When K16 was used as a female parent, positive and significant heterosis of the F1 hybrids was the same as P32. Moreover, significant GCA of the restorer lines, C7, C8 and C9, was observed in some horticultural characteristics. Furthermore, significant differences of the specific combining ability of some characteristics were observed in a few F1 hybrids.","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"73 2","pages":"158-167"},"PeriodicalIF":2.4,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10316304/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9801027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A target cultivar-specific identification system based on the chromatographic printed array strip method for eight prominent Japanese citrus cultivars. 基于层析印刷阵列条带法的8个日本柑橘主要品种特异性鉴定系统。

IF 2.4 4区农林科学

Breeding Science Pub Date : 2023-04-01 DOI: 10.1270/jsbbs.22065

Mitsutoshi Okamoto, Yuki Monden, Akiko Shindo, Tomoyuki Takeuchi, Tomoko Endo, Yukinori Shigematsu, Kazuto Takasaki, Hiroshi Fujii, Takehiko Shimada

{"title":"A target cultivar-specific identification system based on the chromatographic printed array strip method for eight prominent Japanese citrus cultivars.","authors":"Mitsutoshi Okamoto, Yuki Monden, Akiko Shindo, Tomoyuki Takeuchi, Tomoko Endo, Yukinori Shigematsu, Kazuto Takasaki, Hiroshi Fujii, Takehiko Shimada","doi":"10.1270/jsbbs.22065","DOIUrl":"https://doi.org/10.1270/jsbbs.22065","url":null,"abstract":"Citrus is a major cultivated crop in Japan, and new cultivars are of great interest in the Japanese and global market. Recently, the infringement of breeders' rights to citrus cultivars bred in Japan has become a problem related to the agricultural product export strategy promoted by the Japanese government. Cultivar identification systems using DNA markers are an effective tool for protecting breeders' rights. Here, a novel target cultivar-specific identification system using the chromatographic printed array strip method was developed for eight prominent Japanese citrus cultivars. A polymorphic InDel fragment specific to each cultivar was explored through the screening of published citrus InDel markers and next-generation sequencing of retrotransposon libraries. The cultivar-specific DNA marker set for each cultivar comprised 1-3 polymorphic InDel fragments in combination with a PCR-positive DNA marker for the ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit gene. The DNA markers were detected within 3 hours from DNA extraction to the detection by the C-PAS4 membrane stick following multiplex PCR. The developed system is superior as a convenient, rapid, and cost-effective DNA diagnostic method during inspection. The proposed target cultivar-specific identification system is expected to serve as an efficient tool for the injunction of suspicious registered cultivars, contributing to the protection of breeders' rights.","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"73 2","pages":"146-157"},"PeriodicalIF":2.4,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10316311/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9801028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

A novel QTL associated with tolerance to cold-induced seed cracking in the soybean cultivar Toyomizuki. 富水大豆耐冷致种子开裂的新QTL。

IF 2.4 4区农林科学

Breeding Science Pub Date : 2023-04-01 DOI: 10.1270/jsbbs.22066

Naoya Yamaguchi, Yumi Sato, Fumio Taguchi-Shiobara, Kazuki Yamashita, Michio Kawasaki, Masao Ishimoto, Mineo Senda

{"title":"A novel QTL associated with tolerance to cold-induced seed cracking in the soybean cultivar Toyomizuki.","authors":"Naoya Yamaguchi, Yumi Sato, Fumio Taguchi-Shiobara, Kazuki Yamashita, Michio Kawasaki, Masao Ishimoto, Mineo Senda","doi":"10.1270/jsbbs.22066","DOIUrl":"https://doi.org/10.1270/jsbbs.22066","url":null,"abstract":"Low temperatures after flowering cause seed cracking (SC) in soybean. Previously, we reported that proanthocyanidin accumulation on the dorsal side of the seed coat, controlled by the I locus, may lead to cracked seeds; and that homozygous IcIc alleles at the I locus confer SC tolerance in the line Toiku 248. To discover new genes related to SC tolerance, we evaluated the physical and genetic mechanisms of SC tolerance in the cultivar Toyomizuki (genotype II). Histological and texture analyses of the seed coat revealed that the ability to maintain hardness and flexibility under low temperature, regardless of proanthocyanidin accumulation in the dorsal seed coat, contributes to SC tolerance in Toyomizuki. This indicated that the SC tolerance mechanism differed between Toyomizuki and Toiku 248. A quantitative trait loci (QTL) analysis of recombinant inbred lines revealed a new, stable QTL related to SC tolerance. The relationship between this new QTL, designated as qCS8-2, and SC tolerance was confirmed in residual heterozygous lines. The distance between qCS8-2 and the previously identified QTL qCS8-1, which is likely the Ic allele, was estimated to be 2-3 Mb, so it will be possible to pyramid these regions to develop new cultivars with increased SC tolerance.","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"73 2","pages":"204-211"},"PeriodicalIF":2.4,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10316309/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10159990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0