Breeding Science最新文献_第7页

Development of genomic and genetic resources facilitating molecular genetic studies on untapped Myanmar rice germplasms. 开发基因组和遗传资源，促进对尚未开发的缅甸水稻种质进行分子遗传研究。

IF 2 4区农林科学

Breeding Science Pub Date : 2024-04-01 Epub Date: 2024-03-22 DOI: 10.1270/jsbbs.23077

Tomoyuki Furuta, Ohm Mar Saw, Sandar Moe, Khin Thanda Win, Moe Moe Hlaing, Aye Lae Lae Hlaing, Min San Thein, Hideshi Yasui, Motoyuki Ashikari, Atsushi Yoshimura, Yoshiyuki Yamagata

{"title":"Development of genomic and genetic resources facilitating molecular genetic studies on untapped Myanmar rice germplasms.","authors":"Tomoyuki Furuta, Ohm Mar Saw, Sandar Moe, Khin Thanda Win, Moe Moe Hlaing, Aye Lae Lae Hlaing, Min San Thein, Hideshi Yasui, Motoyuki Ashikari, Atsushi Yoshimura, Yoshiyuki Yamagata","doi":"10.1270/jsbbs.23077","DOIUrl":"10.1270/jsbbs.23077","url":null,"abstract":"To counteract the growing population and climate changes, resilient varieties adapted to regional environmental changes are required. Landraces are valuable genetic resources for achieving this goal. Recent advances in sequencing technology have enabled national seed/gene banks to share genomic and genetic information from their collections including landraces, promoting the more efficient utilization of germplasms. In this study, we developed genomic and genetic resources for Myanmar rice germplasms. First, we assembled a diversity panel consisting of 250 accessions representing the genetic diversity of Myanmar indica varieties, including an elite lowland variety, Inn Ma Yebaw (IMY). Our population genetic analyses illustrated that the diversity panel represented Myanmar indica varieties well without any apparent population structure. Second, de novo genome assembly of IMY was conducted. The IMY assembly was constructed by anchoring 2888 contigs, which were assembled from 30× coverage of long reads, into 12 chromosomes. Although many gaps existed in the IMY genome assembly, our quality assessments indicated high completeness in the gene-coding regions, identical to other near-gap-free assemblies. Together with dense variant information, the diversity panel and IMY genome assembly will facilitate deeper genetic research and breeding projects that utilize the untapped Myanmar rice germplasms.","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"74 2","pages":"124-137"},"PeriodicalIF":2.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11442107/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142361092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interaction between genetic regions responsible for the starch properties in non-glutinous rice varieties in Hokkaido, Japan. 日本北海道非糯稻品种淀粉特性遗传区域之间的相互作用。

IF 2 4区农林科学

Breeding Science Pub Date : 2024-04-01 Epub Date: 2024-03-22 DOI: 10.1270/jsbbs.23087

Tomohito Ikegaya

{"title":"Interaction between genetic regions responsible for the starch properties in non-glutinous rice varieties in Hokkaido, Japan.","authors":"Tomohito Ikegaya","doi":"10.1270/jsbbs.23087","DOIUrl":"10.1270/jsbbs.23087","url":null,"abstract":"Starch properties are the major determinants of grain quality and food characteristics in rice (Oryza sativa L.). Understanding the interactions between genetic regions responsible for starch properties will lead to the development of rice cultivars with desirable characteristics. This study investigated the genetic effect and interaction between qAC9.3, a low-amylose quantitative trait locus (QTL), and the genetic region around Starch branching enzyme IIb (SbeIIb). Both these factors are responsible for the starch properties of the Hokkaido breeding population. The amylose content, pasting temperature, and amylopectin chain-length distribution were compared using F5 lines derived from the cross between the lower amylose content and lower pasting temperature strain 'Hokkai332 (qAC9.3, SbeIIb)' and the higher amylose content and higher pasting temperature variety 'Kitagenki (-, SbeIIbsr )'. The qAC9.3 genotype exhibited low amylose content and reduced the hardness of boiled rice but increased the ratio of amylopectin long chains and did not alter the pasting temperature. In contrast, the SbeIIb genotype was associated with pasting temperature but did not affect the amylose content and hardness of boiled rice. It was suggested that appropriately selecting genotypes of these genetic regions and QTL would allow the fine-tuning of starch properties of cooked rice suitable for future demand.","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"74 2","pages":"159-165"},"PeriodicalIF":2.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11442103/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142361094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polyploid QTL-seq revealed multiple QTLs controlling steamed tuber texture and starch gelatinization temperature in sweetpotato. 多倍体 QTL-seq 发现了多个控制甘薯蒸煮块茎质地和淀粉糊化温度的 QTLs。

IF 2 4区农林科学

Breeding Science Pub Date : 2024-04-01 Epub Date: 2024-02-29 DOI: 10.1270/jsbbs.23060

Hiromoto Yamakawa, Tatsumi Mizubayashi, Masaru Tanaka

{"title":"Polyploid QTL-seq revealed multiple QTLs controlling steamed tuber texture and starch gelatinization temperature in sweetpotato.","authors":"Hiromoto Yamakawa, Tatsumi Mizubayashi, Masaru Tanaka","doi":"10.1270/jsbbs.23060","DOIUrl":"10.1270/jsbbs.23060","url":null,"abstract":"Sweetpotato (Ipomoea batatas) includes diverse cultivars with flesh textures ranging from dry to moist. Moist-fleshed cultivars often contain starch with a lower gelatinization temperature (GT). To elucidate the genetic determinants of flesh texture and starch GT, we conducted a QTL analysis using F1 progenies obtained from a cross between dry-fleshed and moist-fleshed cultivars, 'Benikomachi' (BK) and 'Amahazuki' (AH), by using an updated polyploid QTL-seq pipeline. Flesh texture was assessed based on the wet area ratio (WAR) observed on the cut surface of steamed tubers, as progenies with dry and moist flesh exhibited low and high WAR values, respectively, demonstrating a strong correlation. Three QTLs were found to regulate the WAR. Notably, two AH-derived alleles at 4.30 Mb on Itr_chr05 and 21.01 Mb on Itr_chr07, along with a BK-derived allele at 2.89 Mb on Itr_chr15, were associated with increased WAR. Starch GT, which displayed no correlation with either flesh texture or WAR, was distinctly influenced by two QTLs: a GT-increasing BK-derived allele at 1.74 Mb on Itr_chr05 and a GT-decreasing AH-derived allele at 30.16 Mb on Itr_chr12. Consequently, we developed DNA markers linked to WAR, offering a promising avenue for the targeted breeding of sweetpotato with the desired flesh textures.","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"74 2","pages":"103-113"},"PeriodicalIF":2.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11442106/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142361097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diversity of salt tolerance in Vigna nakashimae, wild related species of the azuki bean (Vigna angularis). 红豆（Vigna angularis）的野生近缘种 Vigna nakashimae 的耐盐多样性。

IF 2 4区农林科学

Breeding Science Pub Date : 2024-04-01 Epub Date: 2024-03-29 DOI: 10.1270/jsbbs.23050

Eri Ogiso-Tanaka, Sompong Chankaew, Takehisa Isemura, Rusama Marubodee, Alisa Kongjaimun, Akiko Baba-Kasai, Kazutoshi Okuno, Hiroshi Ehara, Norihiko Tomooka

{"title":"Diversity of salt tolerance in Vigna nakashimae, wild related species of the azuki bean (Vigna angularis).","authors":"Eri Ogiso-Tanaka, Sompong Chankaew, Takehisa Isemura, Rusama Marubodee, Alisa Kongjaimun, Akiko Baba-Kasai, Kazutoshi Okuno, Hiroshi Ehara, Norihiko Tomooka","doi":"10.1270/jsbbs.23050","DOIUrl":"10.1270/jsbbs.23050","url":null,"abstract":"Vigna nakashimae is a wild species closely related to the azuki bean (V. angularis), with salt-tolerance abilities. The present study aimed to explore the genetic and salt tolerance diversity within the species, by evaluating the phylogenetic relationships of 55 accessions of V. nakashimae including 25 newly collected from the Gotō Islands and Iki in Nagasaki Prefecture, Japan. We conducted salt-tolerance analysis for 48 of the accessions, including 18 of the newly collected accessions. Phylogenetic analysis based on single-nucleotide polymorphisms obtained from MIGseq and RADseq analyses revealed the genetic diversity of V. nakashimae to reflect the geographic arrangement of the habitat islands. Korean accessions formed one clade, while Japanese accessions predominantly grouped into Uku Island and Fukue Island subclades. Within this population, we identified \"G4-2\" (JP248291) as the most salt tolerant, surpassing even the previously reported \"Ukushima\" accession. Both accessions collected from Uku Island, with accessions belonging to the Uku Island subclade exhibiting a strong trend of salt tolerance. Our results strongly suggest the occurrence of genetic mutations conferring enhanced salt tolerance in specific clade and region. This study highlights the potential of genetic analyses for identifying regions suitable for collecting valuable genetic resources for stress tolerance.","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"74 2","pages":"166-172"},"PeriodicalIF":2.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11442110/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142362682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the landscape of public attitudes towards gene-edited foods in Japan. 探索日本公众对基因编辑食品的态度。

IF 2 4区农林科学

Breeding Science Pub Date : 2024-03-01 Epub Date: 2024-02-22 DOI: 10.1270/jsbbs.23047

Tomiko Yamaguchi, Kazune Ezaki, Kyoko Ito

{"title":"Exploring the landscape of public attitudes towards gene-edited foods in Japan.","authors":"Tomiko Yamaguchi, Kazune Ezaki, Kyoko Ito","doi":"10.1270/jsbbs.23047","DOIUrl":"10.1270/jsbbs.23047","url":null,"abstract":"The success or failure of food technologies in society depends to a large extent on the public interest, concerns, images, and expectations surrounding them. This paper delves into the landscape of public attitudes towards gene-edited foods in Japan, exploring the reasons behind the acceptance or rejection of these products. A literature review and preliminary findings from a survey conducted in Japan in 2022, aim to identify key issues crucial for evaluating societal acceptance of gene-edited foods. The study showed that the public view gene-edited foods as somewhat unnatural, but upon closer examination, significant variation in attitudes was observed among respondents. Some respondents expressed a favorable perception towards gene-edited foods, particularly those that benefit consumers, while others expressed concerns about its perceived artificiality. Moreover, a significant number of respondents displayed indifference or lack of clear perspective regarding gene-edited foods. These findings reflect the complex relationship between public attitudes, naturalness, and social acceptance of gene-edited foods. Furthermore, the study indicates the importance of paying close attention to those who refrain from expressing their viewpoints in the survey. This nuanced landscape warrants further exploration.","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"74 1","pages":"11-21"},"PeriodicalIF":2.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11375427/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142153132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Global regulatory trends of genome editing technology in agriculture and food. 农业和食品基因组编辑技术的全球监管趋势。

IF 2 4区农林科学

Breeding Science Pub Date : 2024-03-01 Epub Date: 2024-02-22 DOI: 10.1270/jsbbs.23046

Masashi Tachikawa, Makiko Matsuo

引用次数: 0

Accelerating the development of genome-edited crops and the establishment of utilization infrastructure. 加快基因组编辑作物的开发和利用基础设施的建立。

IF 2 4区农林科学

Breeding Science Pub Date : 2024-03-01 DOI: 10.1270/jsbbs.74.1

Hiroshi Ezura

引用次数: 0

Scarless genome editing technology and its application to crop improvement. 无痕基因组编辑技术及其在作物改良中的应用。

IF 2 4区农林科学

Breeding Science Pub Date : 2024-03-01 Epub Date: 2024-03-09 DOI: 10.1270/jsbbs.23045

Kazuya Ikeda

引用次数: 0

Genome editing of DWARF and SELF-PRUNING rapidly confers traits suitable for plant factories while retaining useful traits in tomato. DWARF 和 SELF-PRUNING 的基因组编辑可快速赋予番茄适合植物工厂的性状，同时保留番茄的有用性状。

IF 2 4区农林科学

Breeding Science Pub Date : 2024-03-01 Epub Date: 2024-04-04 DOI: 10.1270/jsbbs.23063

Ai Nagamine, Hiroshi Ezura

{"title":"Genome editing of DWARF and SELF-PRUNING rapidly confers traits suitable for plant factories while retaining useful traits in tomato.","authors":"Ai Nagamine, Hiroshi Ezura","doi":"10.1270/jsbbs.23063","DOIUrl":"10.1270/jsbbs.23063","url":null,"abstract":"Plant factories with artificial light are less affected than open-air areas to environmental factors in crop cultivation and are attracting attention as one of the solutions to the world's food problems. However, the cost of cultivation in plant factories is higher than open-air cultivation, and currently, profitable factory-grown crop varieties are limited to those that are small or have a short growing period. Tomatoes are one of the main crops consumed around the world, but due to their large plant height and width, they are not yet suitable for mass production in plant factories. In this study, the DWARF (D) and SELF-PRUNING (SP) genes of the GABA hyperaccumulating tomato variety #87-17 were genome-edited by the CRISPR-Cas9 method to produce dwarf tomato plants. The desired traits were obtained in the T1 genome-edited generation, and the fruit traits were almost the same as those of the original variety. On the other hand, the F2 cross between #87-17 and Micro-Tom containing the d and sp mutations was dwarfed, but the fruit phenotype was a mixture of the traits of the two varieties. This indicates that genome editing of these two genes using CRISPR-Cas9 can efficiently impart traits suitable for plant factory cultivation while retaining the useful traits of the original cultivar.","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":"74 1","pages":"59-72"},"PeriodicalIF":2.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11375428/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142153133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cas12a and MAD7, genome editing tools for breeding. 用于育种的基因组编辑工具 Cas12a 和 MAD7。

IF 2 4区农林科学

Breeding Science Pub Date : 2024-03-01 Epub Date: 2024-02-22 DOI: 10.1270/jsbbs.23049

Shunya Hozumi, Yi-Chen Chen, Tatsuya Takemoto, Shun Sawatsubashi

引用次数: 0