Studies of potato resistance to Globodera rostochiensis revealed novel alleles for 57R marker.

IF 2 4区农林科学 Q2 AGRONOMY

Breeding Science Pub Date : 2023-06-01 Epub Date: 2023-07-07 DOI:10.1270/jsbbs.22094

Anna Ivanova-Pozdejeva, Liina Jakobson, Kai Ilves, Agnes Kivistik, Liina Kann, Jekaterina Aida, Liisa Kübarsepp, Terje Tähtjärv, Kristiina Laanemets

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引用次数: 0

Abstract

Globodera rostochiensis resistance has been an important trait in potato (Solanum tuberosum) breeding for decades. Our aim was to complement phenotypic testing with genetic marker analysis. We analysed the results of G. rostochiensis resistance greenhouse testing in 4601 tubers of 2918 breeding clones from 11 years. Applicability of H1 gene markers TG689 and 57R was compared. We implemented the latter with the positive predictive value of 99.1% and negative predictive value of 60.0% into the breeding scheme. The 57R marker alleles of 22 Estonian cultivars and 470 breeding clones were determined. Two unique 57R alleles, 57R-887 and 57R-1155, were found in Estonian cultivar 'Anti'. The 887 bp allele has two deletions (14 bp and 490 bp) accompanied by several other indels and SNPs within the 57R marker region. The 1155 bp allele has three deletions (7 bp, 20 bp and 210 bp) accompanied by several other indels and SNPs within the same region. Partial resistance to G. rostochiensis in 'Anti' suggests that the newly described alleles could affect the H1-mediated resistance directly or indirectly.

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对马铃薯抗玫瑰球藻的研究揭示了57R标记的新等位基因。

近几十年来，马铃薯（Solanum tuberosum）的抗病性一直是马铃薯育种中的一个重要性状。我们的目的是用遗传标记分析来补充表型测试。我们分析了11年来2918个繁殖无性系中4601个块茎对罗斯托赤霉抗性的温室试验结果。比较H1基因标记TG689和57R的适用性。我们将阳性预测值为99.1%、阴性预测值为60.0%的后者实施到育种方案中。测定了爱沙尼亚22个品种和470个无性系的57R标记等位基因。在爱沙尼亚品种Anti中发现了两个独特的57R等位基因57R-887和57R-1155。887bp等位基因在57R标记区内有两个缺失（14bp和490bp），并伴有其他几个indel和SNPs。1155bp等位基因有三个缺失（7bp、20bp和210bp），并在同一区域内伴有其他几个indel和SNPs。在“Anti”中对罗斯托奇G.rostochensis的部分抗性表明，新描述的等位基因可能直接或间接影响H1介导的抗性。

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来源期刊

Breeding Science 农林科学-农艺学

CiteScore

4.90

自引率

4.20%

发文量

审稿时长

1.5 months

期刊介绍： Breeding Science is published by the Japanese Society of Breeding. Breeding Science publishes research papers, notes and reviews related to breeding. Research Papers are standard original articles. Notes report new cultivars, breeding lines, germplasms, genetic stocks, mapping populations, database, software, and techniques significant and useful for breeding. Reviews summarize recent and historical events related breeding. Manuscripts should be submitted by corresponding author. Corresponding author must have obtained permission from all authors prior to submission. Correspondence, proofs, and charges of excess page and color figures should be handled by the corresponding author.