Brazilian oral research最新文献

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Oral manifestations of COVID-19 vaccinated individuals, post-infection, and different variants: a Brazilian population study. COVID-19疫苗接种个体、感染后和不同变体的口腔表现:巴西人群研究
IF 1.3 4区 医学
Brazilian oral research Pub Date : 2025-08-04 eCollection Date: 2025-01-01 DOI: 10.1590/1807-3107bor-2025.vol39.078
Larissa Di Carvalho Melo, Juliana Amorim Dos Santos, Bruna Bastos Silveira, Vitória Tavares de Castro, Ana Gabriela Costa Normando, Ana Carolina Prado-Ribeiro, Alan Roger Santos-Silva, Fabiana Vargas-Ferreira, Eliete Neves Silva Guerra
{"title":"Oral manifestations of COVID-19 vaccinated individuals, post-infection, and different variants: a Brazilian population study.","authors":"Larissa Di Carvalho Melo, Juliana Amorim Dos Santos, Bruna Bastos Silveira, Vitória Tavares de Castro, Ana Gabriela Costa Normando, Ana Carolina Prado-Ribeiro, Alan Roger Santos-Silva, Fabiana Vargas-Ferreira, Eliete Neves Silva Guerra","doi":"10.1590/1807-3107bor-2025.vol39.078","DOIUrl":"10.1590/1807-3107bor-2025.vol39.078","url":null,"abstract":"<p><p>This cross-sectional study aims to investigate the prevalence of oral manifestations in a sample of the Brazilian population with COVID-19. Adults diagnosed with COVID-19 through real-time PCR/serological tests were invited to participate. The online questionnaires were distributed at different times to analyze and compare SARS-CoV-2 variants considering the period of prevalence of these variants in Brazil. A total of 846 participants were included, of whom 539 were diagnosed before the Omicron variant. In total, 47.28% were vaccinated with at least two doses. The prevalence of oral manifestations was 52.6% (95%CI: 49.23-55.95), and the most common manifestations included taste disorder (38.06%; 95%CI: 34.85-41.38), xerostomia (17.61%; 95%CI: 15.19-20.32), and halitosis (11.58%; 95%CI: 9.59-13.92). The prevalence of persistent symptoms in post-COVID-19 was 12.1% (95%CI: 10.0-14.4) for taste disorder and 5.4% (95%CI: 4.1-7.1) for xerostomia. A significant association was found between females and persistent taste disorder (p = 0.0084) and oral manifestation and depression/anxiety (OR = 1.855, 95%CI: 1.267-2.717, p = 0.002), worse oral hygiene (OR = 1.729, 95%CI: 1.189-2.516, p = 0.004), and medication use (OR = 1.630, 95%CI: 1.123-2.367, p = 0.010) (p < 0.0001). In the Alpha, Beta, Gamma, and Delta variants, compared with the Omicron variant, taste disorder and xerostomia were less present when toothbrushing habits remained unchanged or improved (p < 0.0001). Oral manifestations in patients with COVID-19 were associated with depression/anxiety, worse oral hygiene, and medication, all of which reinforce its multifactorial etiopathogenesis.</p>","PeriodicalId":9240,"journal":{"name":"Brazilian oral research","volume":"39 ","pages":"e078"},"PeriodicalIF":1.3,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12323848/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144788258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Production and characterization of feldspathic-muscovite glass composite for dental prosthesis. 口腔修复用长石-白云母玻璃复合材料的制备与表征。
IF 1.3 4区 医学
Brazilian oral research Pub Date : 2025-08-04 eCollection Date: 2025-01-01 DOI: 10.1590/1807-3107bor-2025.vol39.066
Fernanda Paes de Figueiredo Costa Pietoso, Shirleny Fontes Santos, Lucas Hian da Silva, Paulo Francisco Cesar
{"title":"Production and characterization of feldspathic-muscovite glass composite for dental prosthesis.","authors":"Fernanda Paes de Figueiredo Costa Pietoso, Shirleny Fontes Santos, Lucas Hian da Silva, Paulo Francisco Cesar","doi":"10.1590/1807-3107bor-2025.vol39.066","DOIUrl":"10.1590/1807-3107bor-2025.vol39.066","url":null,"abstract":"<p><p>The principal objective of this studywas to produce and characterize a machinable glass ceramic containing muscovite-mica as the main crystalline phase to be used as a dental restorative material. The secondary objective was to evaluate the use of muscovite-mica to improve machinability and generate a toughening mechanism in the experimental glass ceramic. After fine milling of a feldspathic glass frit was milled and then mixed with muscovite-mica, die-pressed, and sintered under vacuum at 850 to 1,150 °C. The resulting sintered composite was characterized by X-ray diffraction, scanning electron microscopy, and had its fracture toughness evaluated by micro-indentation. The results were as follows: (a) improved thermal stability of muscovite-mica crystals in the composite; (b) improved leucite crystallization in the feldspathic glass matrix by increasing sintering temperature in the studied range ; (c) the composites consisting of glass + 10% muscovite and glass + 20% muscovite sintered at 1,050°C presented fracture toughness values of 9.0 ± 1.2 and 8.4 ± 0.6 MPa.m1/2, respectively, which are higher than those found in the literature for glass ceramics. Feldspar frit blocks with addition of muscovite-mica (up to 20%) can be densified at temperatures between 1,050 and 1,150°C. This material was stable at a temperature substantially higher than the stability limit of pure muscovite and also showed indentation fracture toughness values greater than those reported in the literature for other glass ceramics.</p>","PeriodicalId":9240,"journal":{"name":"Brazilian oral research","volume":"39 ","pages":"e066"},"PeriodicalIF":1.3,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12323846/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144788259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Accessibility for people with motor disabilities at CEOs in Brazil: an ecological study. 巴西ceo为运动障碍者提供的无障碍环境:一项生态研究。
IF 1.3 4区 医学
Brazilian oral research Pub Date : 2025-08-04 eCollection Date: 2025-01-01 DOI: 10.1590/1807-3107bor-2025.vol39.080
Djalma Antonio de Lima Júnior, Magda Lyce Rodrigues Campos, Elisa Miranda Costa, Rejane Christine de Sousa Queiroz, Ana Margarida Melo Nunes, Nilcema Figueiredo, Paulo Sávio Angeira de Goes, Erika Barbara Abreu Fonseca Thomaz
{"title":"Accessibility for people with motor disabilities at CEOs in Brazil: an ecological study.","authors":"Djalma Antonio de Lima Júnior, Magda Lyce Rodrigues Campos, Elisa Miranda Costa, Rejane Christine de Sousa Queiroz, Ana Margarida Melo Nunes, Nilcema Figueiredo, Paulo Sávio Angeira de Goes, Erika Barbara Abreu Fonseca Thomaz","doi":"10.1590/1807-3107bor-2025.vol39.080","DOIUrl":"10.1590/1807-3107bor-2025.vol39.080","url":null,"abstract":"<p><p>The objective was to compare physical accessibility indicators for people with motor disabilities (PwMD) at dental specialty centers (acronym in Portuguese - CEO) across Brazil during the two evaluation cycles of the Access and Quality Improvement Program (acronym in Portuguese - PMAQ) for CEOs. This ecological study utilized secondary data from the external evaluation of PMAQ-CEO in Cycle I (C1), conducted in 2014, and Cycle II (C2), conducted in 2018, including all CEOs that participated in both cycles (n = 889). The structural items analyzed included corridors and doors adapted for wheelchairs, functional wheelchairs, access ramps with handrails, and bathrooms adapted for PwMD. Latent class transition analysis was applied to identify patterns in physical accessibility among CEOs, selecting the latent status (LS) model based on conceptual interpretability and goodness of fit. The final model identified five LS, labeled as follows: LS1 (most accessible); LS2 (inappropriate doors and bathrooms); LS3 (inappropriate ramps and bathrooms); LS4 (wheelchair unavailability); and LS5 (least accessible). In C1, 33.9% of the CEOs were highly accessible, while 17% were minimally accessible. In C2, these proportions shifted to 69.7% for highly accessible and 6% for minimally accessible. When analyzing the two PMAQ-CEO cycles, improvements in physical accessibility indicators were observed across Brazilian CEOs: corridors (9.7% increase) and doors (4.9%) adapted for wheelchairs; functional wheelchairs (15.7%); access ramps with handrails (38.7%); and bathrooms adapted for PwMD (19.6%). It may be concluded that physical barriers to PwMD in Brazilian CEOs were significantly reduced between 2014 and 2018, improving physical accessibility.</p>","PeriodicalId":9240,"journal":{"name":"Brazilian oral research","volume":"39 ","pages":"e080"},"PeriodicalIF":1.3,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12323847/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144788257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The role of citric acid in denture cleansing: Effects on biofilm reduction and corrosion resistance of Co-Cr alloys. 柠檬酸在义齿清洁中的作用:对Co-Cr合金生物膜还原和耐腐蚀性的影响。
IF 1.5 4区 医学
Brazilian oral research Pub Date : 2025-07-07 eCollection Date: 2025-01-01 DOI: 10.1590/1807-3107bor-2025.vol39.070
Maria Helena Rossy Borges, Luís Fernando Bandeira Miranda, Samuel Santana Malheiros, Ayrton Geroncio Silva, João Vicente Calazans Neto, Mariana Alves Dos Santos, Elidiane Cipriano Rangel, Valentim Adelino Ricardo Barão, Bruna Egumi Nagay
{"title":"The role of citric acid in denture cleansing: Effects on biofilm reduction and corrosion resistance of Co-Cr alloys.","authors":"Maria Helena Rossy Borges, Luís Fernando Bandeira Miranda, Samuel Santana Malheiros, Ayrton Geroncio Silva, João Vicente Calazans Neto, Mariana Alves Dos Santos, Elidiane Cipriano Rangel, Valentim Adelino Ricardo Barão, Bruna Egumi Nagay","doi":"10.1590/1807-3107bor-2025.vol39.070","DOIUrl":"10.1590/1807-3107bor-2025.vol39.070","url":null,"abstract":"<p><p>This in vitro study evaluated the effects of citric acid (CA) on surface properties, biofilm removal, and electrochemical performance of Co-Cr alloys compared to common denture cleansers. Co-Cr discs were divided into five groups based on the decontamination solution: NaCl 0.9% (control), Corega Tabs®, Periogard®, and 10% CA. The surface was characterized at baseline in terms of morphology, topography, and chemical and phase composition. Surface properties, including microhardness, wettability, and roughness, were assessed before and after exposure to each solution. Microbial viability, metabolic activity, and morphology of the polymicrobial biofilm were assessed after treatment to evaluate the efficacy of the decontamination solutions. Electrochemical and morphological evaluations were performed to assess the impact of each solution on the alloy's corrosion process. No significant changes in microhardness were observed (p > 0.05). Decontamination solutions significantly increased surface hydrophilicity (p < 0.05) and roughness, though Ra values remained below the threshold for bacterial colonization. All denture cleansers significantly reduced biofilm viability compared to NaCl (p < 0.05), with no viable colonies post-treatment. The CA group showed a significant reduction in bacterial metabolic activity compared to NaCl and Periogard® (p < 0.05), indicating superior biofilm disruption. Electrochemical tests demonstrated that CA maintained a stable Cr-oxide passive layer, evidenced by nobler OCP values and lower icorr and corrosion rates compared to Periogard® (p < 0.05). SEM images revealed pitting corrosion in all groups, except CA. These findings suggest that CA is a promising and safer alternative for denture care, offering effective antimicrobial action while preserving the electrochemical integrity of Co-Cr alloys.</p>","PeriodicalId":9240,"journal":{"name":"Brazilian oral research","volume":"39 ","pages":"e070"},"PeriodicalIF":1.5,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12237414/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144607410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Influence of luting space settings and polymerization-induced cement shrinkage and deformation/adaptation of CAD/CAM crowns. 凹槽空间设置与聚合诱导水泥收缩变形的影响/ CAD/CAM冠的适应性
IF 1.5 4区 医学
Brazilian oral research Pub Date : 2025-07-07 eCollection Date: 2025-01-01 DOI: 10.1590/1807-3107bor-2025.vol39.074
Leandro Maruki Pereira, Bárbara Inácio de Melo, Aline Aredes Bicalho, Rayssa Rodrigues Pereira, Marcel Santana Prudente, Carlos José Soares, Flávio Domingues das Neves
{"title":"Influence of luting space settings and polymerization-induced cement shrinkage and deformation/adaptation of CAD/CAM crowns.","authors":"Leandro Maruki Pereira, Bárbara Inácio de Melo, Aline Aredes Bicalho, Rayssa Rodrigues Pereira, Marcel Santana Prudente, Carlos José Soares, Flávio Domingues das Neves","doi":"10.1590/1807-3107bor-2025.vol39.074","DOIUrl":"10.1590/1807-3107bor-2025.vol39.074","url":null,"abstract":"<p><p>This study aimed to evaluate the adaptation of CAD/CAM crowns and the impact of resin cement polymerization contraction on crown deformation under different cementation configurations. Thirty human molars were randomly divided into three groups (n = 10) to manufacture ceramic crowns with three different cementation space configurations: LD40 (40 μm), LD80 (80 μm), and LD160 (160 μm). The samples were subjected to computerized microtomography scanning for measuring internal and marginal adaptation, and internal fit was analyzed at occlusal, axial, axiogingival points. Five light-curing protocols were used to verify the wavelength spectrum peak of the curing device and the irradiance delivered using two situations: with 0 mm sensor distance and through a ceramic of differing thickness, and an external crown deformation (ECD) test. The highest statistically significant vertical fit was obtained for group LD40, independent of region. LD160 yielded the highest ECD values, independent of positioning of the buccal and distal strain gauge. The highest internal fit was observed at occlusal and axial points of the LD160 group (P<0.001), and LD80 yielded the lowest values at the axiogingival point (p = 0.003, p = 0.006). The irradiance delivered was reduced significantly upon interposition of the ceramic block to the sensor. The peak wavelength for curing was similar among the LD groups. The luting space configurations significantly influenced vertical fit and the ECD results for the CAD/CAM ceramics owing to polymerization shrinkage. Finally, variations in ceramic thickness at different sites affected both the irradiance by the curing device and ECD results.</p>","PeriodicalId":9240,"journal":{"name":"Brazilian oral research","volume":"39 ","pages":"e074"},"PeriodicalIF":1.5,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12237415/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144607408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bleaching at acidic and basic pH for enamel and dentin whitening using internal and external techniques. 用内部和外部技术在酸性和碱性pH下漂白牙釉质和牙本质。
IF 1.5 4区 医学
Brazilian oral research Pub Date : 2025-07-07 eCollection Date: 2025-01-01 DOI: 10.1590/1807-3107bor-2025.vol39.073
Thais Regina Dias Paini, Monique de Souza, Lidiane Vizioli de Castro-Hoshino, Isolde Terezinha Santos Previdelli, Antonio Carlos Bento, Francielle Sato, Fernanda Tsuzuki, Raquel Sano Suga Terada, Mauro Luciano Baesso, Renata Corrêa Pascotto
{"title":"Bleaching at acidic and basic pH for enamel and dentin whitening using internal and external techniques.","authors":"Thais Regina Dias Paini, Monique de Souza, Lidiane Vizioli de Castro-Hoshino, Isolde Terezinha Santos Previdelli, Antonio Carlos Bento, Francielle Sato, Fernanda Tsuzuki, Raquel Sano Suga Terada, Mauro Luciano Baesso, Renata Corrêa Pascotto","doi":"10.1590/1807-3107bor-2025.vol39.073","DOIUrl":"10.1590/1807-3107bor-2025.vol39.073","url":null,"abstract":"<p><p>This study aims to evaluate in vitro the whitening effects of gels containing hydrogen peroxide at acidic and basic pH using internal and external techniques. Forty artificially darkened bovine incisors were split into four groups: bleaching on the buccal surface with HPE (HP Blue 35%, FGM), at basic pH; PBE (Potenza Bianco 38%, PHS), at acidic pH; bleaching in the pulp chamber with HPI at basic pH; and with PBI at acidic pH. CIELab color parameters, microhardness, roughness, enamel demineralization (FT-Raman), and surface topography (SEM) were evaluated in enamel/dentin blocks after bleaching (T0) and seven days after immersion in saliva (T7). Bleaching agent diffusion was evaluated after 30 minutes, while pH was measured throughout the application period. Physicochemical analysis showed a reduction in the mineral-matrix ratio in both groups after bleaching and at 7 days. Roughness increased over time in the PBE and HPE groups. Porosity increased, but decreased after 7 days of immersion in saliva. Bleaching gels were different at T0 and T7, with acidic pH gel promoting greater whitening (T7) and enamel roughness. Raman scanning demonstrated that the bleaches propagated across the enamel layer with increased concentration at the dentin-enamel-junction and decreasing diffusion gradient along the dentin. The gel with acidic pH promoted a greater increase in enamel roughness and the 7-day immersion in saliva was not enough to restore initial roughness in either gel. The gel with basic pH showed a higher pH decay and a greater diffusion capacity in the dentin.</p>","PeriodicalId":9240,"journal":{"name":"Brazilian oral research","volume":"39 ","pages":"e073"},"PeriodicalIF":1.5,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12237417/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144607405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Tropical almond antioxidant gels mitigate the adverse effects of strong whitening agents on immediate bonding. 热带杏仁抗氧化凝胶减轻了强增白剂对直接结合的不利影响。
IF 1.5 4区 医学
Brazilian oral research Pub Date : 2025-07-07 eCollection Date: 2025-01-01 DOI: 10.1590/1807-3107bor-2025.vol39.072
Verónica Cecilia Mejía, Vitória Moraes Marques, Tamara Gonçalves de Araújo, Lara Cecília de Moura, Ana Cristina de Mello Fiallos, Mary Anne Sampaio de Melo, Sérgio Lima Santiago, Vanara Florêncio Passos
{"title":"Tropical almond antioxidant gels mitigate the adverse effects of strong whitening agents on immediate bonding.","authors":"Verónica Cecilia Mejía, Vitória Moraes Marques, Tamara Gonçalves de Araújo, Lara Cecília de Moura, Ana Cristina de Mello Fiallos, Mary Anne Sampaio de Melo, Sérgio Lima Santiago, Vanara Florêncio Passos","doi":"10.1590/1807-3107bor-2025.vol39.072","DOIUrl":"10.1590/1807-3107bor-2025.vol39.072","url":null,"abstract":"<p><p>Radicals from tooth whitening products can reduce bond strength, posing challenges for dentists when a bonding procedure must be performed in teeth immediately after whitening. This study aimed to evaluate the antioxidant activity (AA) of Terminalia catappa Linn (TCL) leaf extract as a potential agent to mitigate the negative impact of high-concentration whitening agents on immediate bonding performance. The AA of green and ripe leaf extracts was measured using the 2,2-di-phenyl-1-picrylhydrazyl radical scavenging capacity assay (DPPH). To determine the TCL influence on bond strength, six groups of bovine enamel blocks (n=10) were created. Group 1 was the positive control (unbleached enamel), while Group 2 was the negative control (no antioxidant treatment). Groups 3 to 6 were bleached and treated with 10% sodium ascorbate (SA) or 0.1%, 0.2%, and 0.3% TCL gels. Adhesion was conducted using a two-step conventional system and dental composite resin. Microtensile testing was performed after 24 hours, and data were analyzed by one-way ANOVA with Tukey's post hoc test (p > 0.05). From the DPPH assay, results with IC50 < 50 µg/mL indicate high AA for all tested extracts. This method established a difference of around 12 times more AA for the TCL-hydroalcoholic extract of green leaf to the aqueous extract of ripe leaves. There were no significant differences in bond strength among groups treated with TCL-hydroalcoholic extract of green leaf gels (p > 0.05) and unbleached enamel. Tropical almond-derived antioxidant gels emerge as a promising strategy to enhance immediate bond strength on enamel after high-concentration in-office whitening treatments.</p>","PeriodicalId":9240,"journal":{"name":"Brazilian oral research","volume":"39 ","pages":"e072"},"PeriodicalIF":1.5,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12237408/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144607411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
EDTA enhances cementum-like tissue formation, TGF-β1 & VEGF in rat molars during regenerative endodontics. EDTA促进再生牙髓治疗大鼠磨牙骨质样组织形成、TGF-β1和VEGF。
IF 1.5 4区 医学
Brazilian oral research Pub Date : 2025-07-07 eCollection Date: 2025-01-01 DOI: 10.1590/1807-3107bor-2025.vol39.068
Alexandre Henrique Dos Reis-Prado, Isabela Joane Prado Silva, Juliana Goto, Nathália Evelyn da Silva Machado, Gleide Fernandes de Avelar, Juliano Douglas Silva Albergaria, Raphael Escorsim Szawka, Marco Cícero Bottino, Luciano Tavares Angelo Cintra, Edilson Ervolino, Antônio Paulino Ribeiro-Sobrinho, Francine Benetti
{"title":"EDTA enhances cementum-like tissue formation, TGF-β1 & VEGF in rat molars during regenerative endodontics.","authors":"Alexandre Henrique Dos Reis-Prado, Isabela Joane Prado Silva, Juliana Goto, Nathália Evelyn da Silva Machado, Gleide Fernandes de Avelar, Juliano Douglas Silva Albergaria, Raphael Escorsim Szawka, Marco Cícero Bottino, Luciano Tavares Angelo Cintra, Edilson Ervolino, Antônio Paulino Ribeiro-Sobrinho, Francine Benetti","doi":"10.1590/1807-3107bor-2025.vol39.068","DOIUrl":"10.1590/1807-3107bor-2025.vol39.068","url":null,"abstract":"<p><p>This study investigated the influence of ethylenediaminetetraacetic acid (EDTA) irrigation on cementum-like tissue formation and TGF-β1, FGF-2, and VEGF immunolabeling during regenerative endodontic procedures (REPs) in immature rat molars. The lower first molars of 12 four-week-old male rats (80 g) underwent pulpectomy in the mesial canal and were randomly categorized into two experimental groups (n = 6): sodium hypochlorite (NaOCl) - irrigated for 5 min with 2.5% NaOCl; and NaOCl-EDTA - irrigated with 2.5% NaOCl, followed by 5 min of 17% EDTA. After inducing bleeding with a size 10 K-file, the cavities were sealed. Untreated molars served as control-15d (n = 3) and immediate control (n = 3). Either immediately or after 15 days, the animals were euthanized, and the teeth were collected for histomorphometric and immunohistochemical (TGF-β1, FGF-2, and VEGF) analysis. The results were analyzed by the Mann-Whitney U-test (p < 0.05). Histomorphometric analysis revealed increased cementum-like tissue formation in the NaOCl-EDTA group compared with that in the NaOCl group (p < 0.05). Regarding growth factor immunolabeling, the NaOCl-EDTA group exhibited enhanced TGF-β1 and VEGF immunolabeling in the root tip area and the center region of the apical third of the pulp tissue, compared with that in the NaOCl group (p < 0.05); however, no significant difference was observed in FGF-2 (p > 0.05). In conclusion, the use of EDTA in REPs positively affected the formation of cementum-like tissue and TGF-β1 and VEGF in the apical region but did not influence FGF-2.</p>","PeriodicalId":9240,"journal":{"name":"Brazilian oral research","volume":"39 ","pages":"e068"},"PeriodicalIF":1.5,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12237413/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144607407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Physicochemical and antibiofilm properties of new ready-to-use and powder/gel bioceramic endodontic sealers. 新型即用型和粉末/凝胶型生物陶瓷牙髓密封剂的物理化学和抗菌膜性能。
IF 1.5 4区 医学
Brazilian oral research Pub Date : 2025-07-07 eCollection Date: 2025-01-01 DOI: 10.1590/1807-3107bor-2025.vol39.076
Jéssica Arielli Pradelli, José Leandro de Abreu Jampani, Airton Oliveira Santos-Junior, Mario Tanomaru-Filho, Juliane Maria Guerreiro-Tanomaru
{"title":"Physicochemical and antibiofilm properties of new ready-to-use and powder/gel bioceramic endodontic sealers.","authors":"Jéssica Arielli Pradelli, José Leandro de Abreu Jampani, Airton Oliveira Santos-Junior, Mario Tanomaru-Filho, Juliane Maria Guerreiro-Tanomaru","doi":"10.1590/1807-3107bor-2025.vol39.076","DOIUrl":"10.1590/1807-3107bor-2025.vol39.076","url":null,"abstract":"<p><p>NeoSealer Flo (NSF) is a novel ready-to-use endodontic bioceramic sealer, and NeoMTA2 (NMTA2) is a powder-gel bioceramic used as a repair or filling material. Setting time (ST), pH, solubility, radiopacity, and flow were evaluated in accordance with the ISO 6876:2012 standards for NSF and NMTA2, in comparison with Bio-C Sealer (BCS) and AH Plus Jet (AHP). Bond strength was assessed using the push-out test. Antibiofilm activity was evaluated by a modified direct contact test on Enterococcus faecalis biofilm with fresh sealer eluate and material after setting. Data were analyzed using the t-test, ANOVA, and Tukey post-hoc tests (α = 0.05). AHP exhibited the highest ST value, followed by NMTA2 (p < 0.05). NSF and BCS showed solubility above 3%, while NMTA2 exhibited values lower than 3% (p < 0.05). All materials demonstrated radiopacity above 3 mm Al (p > 0.05). NMTA2 demonstrated flow below ISO-recommendation (p < 0.05). NSF showed the highest bond strength values (p < 0.05). The bioceramic sealers induced higher pH compared with AHP (p < 0.05). NMTA2 exhibited more significant antibiofilm activity after the sealer set (p < 0.05). For fresh sealer eluate, NSF, BCS, and AHP demonstrated higher levels of antibiofilm activity compared with NMTA2 (p < 0.05). NSF and NMTA2 demonstrated the required physicochemical and mechanical properties, as well as antibiofilm activity against E. faecalis. However, NSF and BCS presented higher solubility. NMTA2 presented adequate solubility, however lower flow rates than those recommended by ISO standards.</p>","PeriodicalId":9240,"journal":{"name":"Brazilian oral research","volume":"39 ","pages":"e076"},"PeriodicalIF":1.5,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12237406/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144607409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Clinical evaluation of the effect of 980 nm diode laser and fluoride varnish on dentin hypersensitivity. 980 nm二极管激光联合氟化物清漆治疗牙本质过敏的临床评价。
IF 1.5 4区 医学
Brazilian oral research Pub Date : 2025-07-07 eCollection Date: 2025-01-01 DOI: 10.1590/1807-3107bor-2025.vol39.077
Sérgio Kiyoshi Ishikiriama, Bruno Nicollielo Moreira, Linda Wang, Juliana Fraga Soares Bombonatti, Giovanna Speranza Zabeu, Fabio Antonio Piola Rizzante
{"title":"Clinical evaluation of the effect of 980 nm diode laser and fluoride varnish on dentin hypersensitivity.","authors":"Sérgio Kiyoshi Ishikiriama, Bruno Nicollielo Moreira, Linda Wang, Juliana Fraga Soares Bombonatti, Giovanna Speranza Zabeu, Fabio Antonio Piola Rizzante","doi":"10.1590/1807-3107bor-2025.vol39.077","DOIUrl":"10.1590/1807-3107bor-2025.vol39.077","url":null,"abstract":"<p><p>Dentin hypersensitivity (DH) is a common and challenging clinical condition with limited long-lasting treatments. The objective of this study was to evaluate the effectiveness of 980 nm diode laser treatment, associated or not with fluoride varnish, in the treatment of DH. Sixty volunteers were selected and randomly assigned for treatment following three different protocols (1- treatment with 0.8W diode laser; 2- treatment with 0.8 W diode laser over fluoride varnish; or 3- fluoride varnish only). The 0.8 W diode laser was applied in contact with the exposed roots at 10 Hz, for 30 s, with 99.17J/cm2 energy density, using a zigzag pattern. DH assessment was performed using the visual analog scale (VAS), prior to treatment, immediately after treatment, and at 7, 30, and 180 days after treatment. The data obtained were subjected to two-way repeated-measures analysis of variance (ANOVA) (p <0.05). The varnish group showed a reduction in DH up to 30 days, whereas the laser and laser + varnish groups showed a reduction in DH up to 180 days ,with no difference between them. The laser and laser + varnish groups were superior to the varnish group after 30 days. The treatment of exposed roots with diode laser alone or associated with fluoride varnish, according to the parameters used in this study, was effective in reducing DH up to six months.</p>","PeriodicalId":9240,"journal":{"name":"Brazilian oral research","volume":"39 ","pages":"e077"},"PeriodicalIF":1.5,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12237409/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144607406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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