BMC Plant Biology最新文献

{"title":"Mutations in active surface sites of NtGGPPS1 enhance carotenoid biosynthesis and drought resistance in Nicotiana tabacum.","authors":"Shanshan Song, Yulong Gao, Puqing Fan, Faming Lin, Houliang Zhou, Zhiyang Li, Hang Liu, Hongyi Su, Qiang Zhong, Zhuonan Liu, Xinru Ren, Xiaoran Wang, Yongchun Shi, Yu Zhang, Xiaoquan Zhang, Ran Wang","doi":"10.1186/s12870-025-07337-5","DOIUrl":"10.1186/s12870-025-07337-5","url":null,"abstract":"","PeriodicalId":9198,"journal":{"name":"BMC Plant Biology","volume":"25 1","pages":"1296"},"PeriodicalIF":4.8,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12492537/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic variability in physiological and agronomic traits of newly developed rice lines under well-watered and water-deficit conditions.","authors":"Raghda M Sakran, Mohamed I Ghazy, Mahmoud M Gaballah, Fatma A Hussein, Samah M Aamer, Hasnaa A Ghazy, Sobhi F Lamlom, Ahmed M Abdelghany","doi":"10.1186/s12870-025-07436-3","DOIUrl":"10.1186/s12870-025-07436-3","url":null,"abstract":"","PeriodicalId":9198,"journal":{"name":"BMC Plant Biology","volume":"25 1","pages":"1291"},"PeriodicalIF":4.8,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12490075/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Natural variation in Arabidopsis thaliana highlights a key role of glyoxalase I;2 in detoxifying glucose-derived reactive carbonyl species.","authors":"Maroua Bouzid, Manuel Balparda, Aylin Kerim, Ying Fu, Clarisa E Alvarez, Saleh Alseekh, Alisdair R Fernie, Veronica G Maurino","doi":"10.1186/s12870-025-07238-7","DOIUrl":"10.1186/s12870-025-07238-7","url":null,"abstract":"<p><p>Reactive carbonyl species (RCS) are toxic byproducts of normal metabolism that become more prevalent under oxidative stress. Here, we show that Arabidopsis thaliana ecotypes exhibit natural variation in their ability to detoxify glucose-derived RCS. We identified the IP-Pal-0 ecotype showing enhanced tolerance to glucose-derived RCS via upregulation of the glyoxalase system. In particular, the Viridiplantae-specific isoforms GLXI;2, GLXII;4, and GLXII;5 are highly expressed in IP-Pal-0 when plants are grown in the presence of 2-keto-D-glucose (KDG; glucosone) or methylglyoxal, and protein extracts from these plants display enhanced GLXI activity on KDG than other ecotypes. We identified specific motif/cis-regulatory elements in the GLXI;2 promoter regions of Col-0 and IP-Pal-0 that may underlie the differential expression of GLXI;2 associated with KDG detoxification. IP-Pal-0 GLXI;2 contains two different amino acids compared to Col-0, but these do not affect the basic kinetics of the protein. Interestingly, we found that the simultaneous change of these amino acids also occurs together in the GLXI proteins of some other organisms, suggesting a convergence in the simultaneous change of both amino acid residues. Our findings underscore the importance of Viridiplantae-specific glyoxalase isoforms in detoxifying glucose-derived RCS, particularly KDG, and highlight the promise of harnessing natural genetic diversity in the glyoxalase pathway to enhance plant stress tolerance.</p>","PeriodicalId":9198,"journal":{"name":"BMC Plant Biology","volume":"25 1","pages":"1288"},"PeriodicalIF":4.8,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12490080/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative transcriptome analysis between salt-tolerant and salt-sensitive naked barley landraces under salt stress at the germination stage.","authors":"Zhenzhu Guo, Shuwei Zhang, Guimei Guo, Yu Wang, Longhua Zhou, Chenghong Liu, Zhaxi Luobu, Qijun Bao, Zhiwei Chen","doi":"10.1186/s12870-025-07334-8","DOIUrl":"10.1186/s12870-025-07334-8","url":null,"abstract":"<p><strong>Background: </strong>Salt stress is a major abiotic stress that threatens crop production globally. Among various growth stages, seed germination, crucial for seedling establishment and crop population, is highly sensitive to salt stress. Therefore, it is essential to explore the molecular mechanisms at this stage for the breeding of salt-tolerant crops and global food security.</p><p><strong>Results: </strong>In this study, two salt-tolerant (B080 and B084) and two salt-sensitive (B004 and B005) naked barley landraces were exposed to salt stress for 7 days during germination and their transcriptome were compared to identify differentially expressed genes (DEGs) related to salt stress. In totally, 2348, 1847, 2251, and 1251 DEGs were obtained in B080, B084, B004, and B005, respectively. Then, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment of DEGs were analyzed. Eight DEGs were further validated by qPCR. Although there were only two genes significantly differential expressed, the upregulation and downregulation trends of all genes were consistent with mRNA-seq data.</p><p><strong>Conclusions: </strong>The analysis of DEGs between salt-tolerant and salt-sensitive naked barley landraces under salt stress, as well as their GO and KEGG enrichment, could reveal and enrich the molecular mechanism of salt tolerance in naked barley.</p>","PeriodicalId":9198,"journal":{"name":"BMC Plant Biology","volume":"25 1","pages":"1293"},"PeriodicalIF":4.8,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12492895/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sand burial and precipitation enhancement changed status of tillerous clonal plant Stipa bungeana as dominated species in sandy grassland.","authors":"Yuying Shao, Saina, Lei Wang, Qingguo Cui, Xuehua Ye, Zhenying Huang","doi":"10.1186/s12870-025-07262-7","DOIUrl":"10.1186/s12870-025-07262-7","url":null,"abstract":"","PeriodicalId":9198,"journal":{"name":"BMC Plant Biology","volume":"25 1","pages":"1276"},"PeriodicalIF":4.8,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12486527/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Habitat-driven variability in morphological and anatomical traits of Dactyloctenium aegyptium (Poaceae) in semi-arid regions.","authors":"Naila Mukhtar, Muhammad Waheed, Fahim Arshad, Nidaa Harun, Uzma Amin, Shaheena Umbreen, Abeer Al-Andal, Valisher Sapayev, Malokhat Saidmuratova, Abdul Rahman Osmani, Marina Taheri","doi":"10.1186/s12870-025-07361-5","DOIUrl":"10.1186/s12870-025-07361-5","url":null,"abstract":"<p><strong>Background: </strong>The ability of Dactyloctenium aegyptium (L.) Willd. to adapt to diverse habitats reflects its remarkable ecological plasticity.</p><p><strong>Methods: </strong>This study investigates the morphological and anatomical traits of D. aegyptium across six distinct habitat types in semi-arid regions, which vary in moisture availability, soil texture, and levels of disturbance. We used one-way ANOVA and Principal Component Analysis (PCA) to evaluate habitat-driven differences and identify key patterns of trait variation.</p><p><strong>Results: </strong>We found significant habitat-induced differences in morphological traits such as stem length, root length, leaf length, and plant biomass. Stem length and diameter peaked in sandy and canal bank habitats, while abandoned land exhibited the lowest values. Leaf length was highest in canal bank habitats and lowest in abandoned land. Sandy places supported the highest biomass, reflecting optimal growth conditions, while abandoned land recorded minimal biomass. Anatomical traits displayed habitat-specific adaptations, with roadside habitats exhibiting the thickest root epidermis and sandy places showing the highest endodermis thickness. Leaf anatomical features such as vascular bundle dimensions and bulliform cell density varied significantly, reflecting adaptations to environmental stresses. PCA revealed that plant traits clustered according to habitat type, with traits such as stem length, leaf size, and biomass associated with resource-rich habitats like roadside and agricultural land, indicating habitat-driven differentiation and adaptive plasticity.</p><p><strong>Conclusion: </strong>These findings highlight the strong relationship between habitat variability and morphological and anatomical plasticity in D. aegyptium, providing insights into its adaptive strategies in semi-arid regions. This study contributes to understanding the ecological resilience of grass species under varying environmental conditions and highlights the importance of habitat-driven plasticity in shaping plant traits.</p>","PeriodicalId":9198,"journal":{"name":"BMC Plant Biology","volume":"25 1","pages":"1277"},"PeriodicalIF":4.8,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12487550/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome and metabolome profiling of the medicinal plant Dictamnus dasycarpus reveal key genes involved in quinoline alkaloids biosynthesis and limonoids biosynthesis.","authors":"Jiawei Mao, Jianhan Liu, Yuying Hou, Xue Fang, Xueqi Cheng, Lei Tian, Yue Ma, Haibo Yin, Xiaoxia Chen","doi":"10.1186/s12870-025-07378-w","DOIUrl":"10.1186/s12870-025-07378-w","url":null,"abstract":"<p><strong>Background: </strong>As a member of Rutaceae family, Dictamnus dasycarpus Turcz. represents a prominent medicinal plant and economically valuable crop in traditional Chinese medicine, and is renowned for its therapeutic efficacy in treating dermatological conditions. The pharmacological activity of this species primarily stems from quinoline alkaloids and limonoids, which predominantly accumulate in the taproots. These bioactive compounds serve as critical determinants of both medicinal quality and crop yield. Nevertheless, the molecular mechanisms governing their dynamic accumulation patterns in D. dasycarpus taproots remain uncertain, and the fundamental biochemical basis underlying this process has yet to be elucidated.</p><p><strong>Results: </strong>Metabolomic and transcriptomic analyses were carried out to investigate metabolites and gene expression during the development of D. dasycarpus taproots. The differentially accumulated secondary metabolites (DAMs) mainly included quinoline alkaloids and limonoids, and the accumulation of total alkaloids and total limonoids primarily occurred during 2- and 4-year-old. The differentially expressed genes (DEGs) are related to Glycolysis/Gluconeogenesis, Phenylalanine, tyrosine and tryptophan biosynthesis, Tryptophan metabolism, Terpenoid backbone biosynthesis, Sesquiterpenoid and triterpenoid biosynthesis, which had a close relationship with the accumulation of quinoline alkaloids and limonoids. Furthermore, we identified that some CYP450s, acetyltransferase, isomerase, 2-ODDs and others may play an important role in the process of producing quinoline alkaloids and limonoids.</p><p><strong>Conclusion: </strong>These results elucidated the molecular mechanisms and metabolic changes underlying the dynamic accumulation process occurring in the taproots of D. dasycarpus. These findings provide a theoretical basis for the planting and harvesting of D. dasycarpus.</p>","PeriodicalId":9198,"journal":{"name":"BMC Plant Biology","volume":"25 1","pages":"1282"},"PeriodicalIF":4.8,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12486547/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"OsFLN2 gene knockout reduces leaf water status and photosynthetic performance of rice plants under water deficit.","authors":"Danyel Fernandes Contiliani, Simone Ferreira da Silva, Rafael Vasconcelos Ribeiro, Vitor Nolasco Moraes, Laísa Medeiros Rocha, Greice Lubini, Paula Macedo Nóbile, Tiago Campos Pereira, Silvana Creste","doi":"10.1186/s12870-025-07273-4","DOIUrl":"10.1186/s12870-025-07273-4","url":null,"abstract":"<p><p>Abiotic stresses significantly impact plant growth and productivity. To overcome these challenges, plants employ photosynthetic acclimation mechanisms, enabling them to sustain photosynthetic efficiency under adverse conditions. The OsFLN2 gene has been identified as a key player in chloroplast development and response to heat and salinity stress. However, the physiological effects of OsFLN2 gene knockout and its impact on drought tolerance remain elusive. In this study, we explored the function of OsFLN2 in rice plants under water deficit conditions using a non-transgenic CRISPR knockout mutant. Our findings demonstrate that Osfln2 mutant plants exhibit higher sensitivity to water deficit, characterized by reduced photosynthetic activity and reduced transcriptional responsiveness of plastid genes, such as RbcL and PsaA. As a result, these plants display compromised carboxylation efficiency and reduced leaf water use efficiency at the maximum water deficit. Concluding, our results demonstrate the role of OsFLN2 on physiological acclimation of rice plants under water-limiting conditions.</p>","PeriodicalId":9198,"journal":{"name":"BMC Plant Biology","volume":"25 1","pages":"1266"},"PeriodicalIF":4.8,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12487551/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of graphene oxide nanoparticles for improvement of growth parameters, photosynthetic pigments, and essential oil quality and yield of Lavandula angustifolia Mill. under green manure incorporation.","authors":"Mohammad Asadi, Yousef Nasiri, Farzad Rasouli, Karim Kakaei, Mohammad Reza Morshedloo, Sezai Ercisli, Sona Skrovankova, Jiri Mlcek","doi":"10.1186/s12870-025-07364-2","DOIUrl":"10.1186/s12870-025-07364-2","url":null,"abstract":"","PeriodicalId":9198,"journal":{"name":"BMC Plant Biology","volume":"25 1","pages":"1278"},"PeriodicalIF":4.8,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12487077/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolome and transcriptome based investigation of anthocyanin accumulation in black wolfberry (Lycium ruthenicum) and validation of the function of LrUFGT.","authors":"Ying Chen, Xingyao Fan, Silin Zhang, Yaoyao Ding, Yanan Wang, Sijing Fan, Xu Sun","doi":"10.1186/s12870-025-07302-2","DOIUrl":"10.1186/s12870-025-07302-2","url":null,"abstract":"<p><strong>Background: </strong>Black wolfberry (Lycium ruthenicum Murray) is renowned for its abundant anthocyanins, exhibiting antioxidant, anti-inflammatory, and anti-ageing activities. However, the accumulation patterns and molecular regulation of anthocyanins during fruit maturation are poorly understood. This study examined anthocyanin biosynthesis at three different stages: young fruit (LR-1), color transition (LR-2), and mature fruit (LR-3).</p><p><strong>Results: </strong>The analysis revealed higher levels of anthocyanins in LR-3 (12.83 mg/g) than in LR-2 (9.76 mg/g) and LR-1 (8.41 mg/g), which established a correlation between anthocyanin accumulation and pericarp pigmentation. Antioxidant capacity assays demonstrated an increase in total antioxidant activity and hydroxyl radical scavenging ability during maturation. HPLC profiling identified three anthocyanidin aglycones: cyanidin, pelargonidin and delphinidin. LR-3 exhibited predominant cyanidin accumulation, while LR-1 lacked pelargonidin. Furthermore, the metabolomic analysis revealed that, the accumulation of three specific anthocyanins cyanidin-3,5-di-O-glucoside, cyanidin-3-sophoroside-5-glucoside, and pelargonidin, serves as the primary determinant of color variation in black wolfberry fruits. A transcriptomic investigation revealed the activation of structural genes (LrCHI, LrF3'H, LrF3'5'H) and glycosyltransferase gene LrUFGT. WGCNA and correlation analyses were then performed to identify networks coordinating anthocyanin biosynthesis. Functional characterization confirmed that chloroplast-localized LrUFGT catalysis cyanidin glycosylation to produce cyanidin-3-O-glucoside. Heterologous expression of LrUFGT in yeast led to enhanced tolerance to salinity and drought stress.</p><p><strong>Conclusions: </strong>This multi-omics study provides a mechanistic understanding of anthocyanin regulation in black wolfberry, identifying targets for developing novel germplasms with elevated anthocyanin content. These findings establish a foundation for breeding strategies to enhance the nutritional quality and commercial value of black wolfberry.</p>","PeriodicalId":9198,"journal":{"name":"BMC Plant Biology","volume":"25 1","pages":"1265"},"PeriodicalIF":4.8,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12487292/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}