Comparative transcriptome analysis between salt-tolerant and salt-sensitive naked barley landraces under salt stress at the germination stage.

IF 4.8 2区生物学 Q1 PLANT SCIENCES

BMC Plant Biology Pub Date : 2025-10-02 DOI:10.1186/s12870-025-07334-8

Zhenzhu Guo, Shuwei Zhang, Guimei Guo, Yu Wang, Longhua Zhou, Chenghong Liu, Zhaxi Luobu, Qijun Bao, Zhiwei Chen

{"title":"Comparative transcriptome analysis between salt-tolerant and salt-sensitive naked barley landraces under salt stress at the germination stage.","authors":"Zhenzhu Guo, Shuwei Zhang, Guimei Guo, Yu Wang, Longhua Zhou, Chenghong Liu, Zhaxi Luobu, Qijun Bao, Zhiwei Chen","doi":"10.1186/s12870-025-07334-8","DOIUrl":null,"url":null,"abstract":"Background: Salt stress is a major abiotic stress that threatens crop production globally. Among various growth stages, seed germination, crucial for seedling establishment and crop population, is highly sensitive to salt stress. Therefore, it is essential to explore the molecular mechanisms at this stage for the breeding of salt-tolerant crops and global food security.Results: In this study, two salt-tolerant (B080 and B084) and two salt-sensitive (B004 and B005) naked barley landraces were exposed to salt stress for 7 days during germination and their transcriptome were compared to identify differentially expressed genes (DEGs) related to salt stress. In totally, 2348, 1847, 2251, and 1251 DEGs were obtained in B080, B084, B004, and B005, respectively. Then, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment of DEGs were analyzed. Eight DEGs were further validated by qPCR. Although there were only two genes significantly differential expressed, the upregulation and downregulation trends of all genes were consistent with mRNA-seq data.Conclusions: The analysis of DEGs between salt-tolerant and salt-sensitive naked barley landraces under salt stress, as well as their GO and KEGG enrichment, could reveal and enrich the molecular mechanism of salt tolerance in naked barley.","PeriodicalId":9198,"journal":{"name":"BMC Plant Biology","volume":"25 1","pages":"1293"},"PeriodicalIF":4.8000,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12492895/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"BMC Plant Biology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1186/s12870-025-07334-8","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Salt stress is a major abiotic stress that threatens crop production globally. Among various growth stages, seed germination, crucial for seedling establishment and crop population, is highly sensitive to salt stress. Therefore, it is essential to explore the molecular mechanisms at this stage for the breeding of salt-tolerant crops and global food security.

Results: In this study, two salt-tolerant (B080 and B084) and two salt-sensitive (B004 and B005) naked barley landraces were exposed to salt stress for 7 days during germination and their transcriptome were compared to identify differentially expressed genes (DEGs) related to salt stress. In totally, 2348, 1847, 2251, and 1251 DEGs were obtained in B080, B084, B004, and B005, respectively. Then, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment of DEGs were analyzed. Eight DEGs were further validated by qPCR. Although there were only two genes significantly differential expressed, the upregulation and downregulation trends of all genes were consistent with mRNA-seq data.

Conclusions: The analysis of DEGs between salt-tolerant and salt-sensitive naked barley landraces under salt stress, as well as their GO and KEGG enrichment, could reveal and enrich the molecular mechanism of salt tolerance in naked barley.

查看原文本刊更多论文

盐胁迫下耐盐和盐敏感裸大麦地方品种萌发期转录组比较分析。

背景：盐胁迫是威胁全球作物生产的主要非生物胁迫。在不同的生长阶段中，种子萌发对盐胁迫非常敏感，对幼苗的形成和作物的数量至关重要。因此，在这一阶段探索耐盐作物的分子机制对培育耐盐作物和全球粮食安全至关重要。结果：本研究将两个耐盐品种（B080和B084）和两个盐敏感品种（B004和B005）在萌发期暴露于盐胁迫下7 d，比较了它们的转录组，以鉴定盐胁迫相关的差异表达基因（DEGs）。在B080、B084、B004和B005中共检出2348、1847、2251和1251个deg。然后，对基因本体（GO）和京都基因与基因组百科全书（KEGG）进行了分析。8个deg通过qPCR进一步验证。虽然只有两个基因差异表达显著，但所有基因的上调和下调趋势与mRNA-seq数据一致。结论：分析盐胁迫下耐盐和盐敏感裸大麦地方品种间的deg及GO和KEGG的富集情况，可以揭示和丰富裸大麦耐盐的分子机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

BMC Plant Biology 生物-植物科学

CiteScore

8.40

自引率

3.80%

发文量

539

审稿时长

3.8 months

期刊介绍： BMC Plant Biology is an open access, peer-reviewed journal that considers articles on all aspects of plant biology, including molecular, cellular, tissue, organ and whole organism research.