Biorheology最新文献_第7页

Evaluation of Long-Term Cryostorage of Brain Tissue Sections for Quantitative Histochemistry. 评估用于定量组织化学的脑组织切片的长期冷冻储存。

IF 3.2 4区医学

Biorheology Pub Date : 2017-03-01 Epub Date: 2017-01-12 DOI: 10.1369/0022155416686934

Larissa I Estrada, Amy A Robinson, Ana C Amaral, Eustathia L Giannaris, Nadine C Heyworth, Farzad Mortazavi, Laura B Ngwenya, Debra E Roberts, Howard J Cabral, Ronald J Killiany, Douglas L Rosene

{"title":"Evaluation of Long-Term Cryostorage of Brain Tissue Sections for Quantitative Histochemistry.","authors":"Larissa I Estrada, Amy A Robinson, Ana C Amaral, Eustathia L Giannaris, Nadine C Heyworth, Farzad Mortazavi, Laura B Ngwenya, Debra E Roberts, Howard J Cabral, Ronald J Killiany, Douglas L Rosene","doi":"10.1369/0022155416686934","DOIUrl":"10.1369/0022155416686934","url":null,"abstract":"Storage of tissue sections for long periods allows multiple samples, acquired over months or years, to be processed together, in the same reagents, for quantitative histochemical studies. Protocols for freezer storage of free-floating frozen sections using sucrose with different additives have been reported and assert that storage has no effect on histochemistry, but no quantitative support has been provided. The present study analyzed the efficacy of long-term storage of brain tissue sections at -80C in buffered 15% glycerol. To determine whether histochemical reactivity is affected, we analyzed 11 datasets from 80 monkey brains that had sections stored for up to 10 years. For processing, sections from multiple cases were removed from storage, thawed, and batch-processed at the same time for different histochemical measures, including IHC for neuronal nuclear antigen, parvalbumin, orexin-A, doublecortin, bromodeoxyuridine, the pro-form of brain-derived neurotrophic factor, and damaged myelin basic protein as well as a histochemical assay for hyaluronic acid. Results were quantified using stereology, optical densitometry, fluorescence intensity, or percent area stained. Multiple regression analyses controlling for age and sex demonstrated the general stability of these antigens for up to a decade when stored in 15% glycerol at -80C.","PeriodicalId":9167,"journal":{"name":"Biorheology","volume":"33 1","pages":"153-171"},"PeriodicalIF":3.2,"publicationDate":"2017-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5298458/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74893962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of ovariectomy and Sideritis euboea extract administration on large artery mechanics, morphology, and structure in middle-aged rats. 卵巢切除和黄芪提取物对中年大鼠大动脉力学、形态和结构的影响。

IF 1.1 4区医学

Biorheology Pub Date : 2017-01-01 DOI: 10.3233/BIR-16113

Dimitrios P Sokolis, Constantinos A Dimitriou, Pavlos Lelovas, Nikolaos G Kostomitsopoulos, Ismene A Dontas

{"title":"Effect of ovariectomy and Sideritis euboea extract administration on large artery mechanics, morphology, and structure in middle-aged rats.","authors":"Dimitrios P Sokolis, Constantinos A Dimitriou, Pavlos Lelovas, Nikolaos G Kostomitsopoulos, Ismene A Dontas","doi":"10.3233/BIR-16113","DOIUrl":"https://doi.org/10.3233/BIR-16113","url":null,"abstract":"Background: Arterial function is regulated by estrogen, but no consistent pattern of arterial mechanical remodeling in response to depleted estrogen levels is available.Objective: To examine long-term effects of ovariectomy (OVX) on the mechanical properties, morphology, and histological structure of the carotid artery in middle-aged rats and a potentially protective effect of Sideritis euboea extract (SID), commonly consumed as \"mountain tea\".Methods: 10-month-old female Wistar rats were allocated into control (sham-operated), OVX, OVX+SID, and OVX+MALT (maltodextrin; excipient used for dilution of SID) groups. They were sacrificed after 6 months and their carotid arteries were submitted to inflation/extension tests and to dimensional and histological evaluation.Results: Remodeling in OVX rats was characterized by a decreased in situ axial extension ratio, along with increased opening angle, thickness, and area of the vessel wall and of its medial layer, but unchanged lumen diameter. Compositional changes involved increased elastin/collagen densities. Characterization by the \"four-fiber\" microstructure-motivated model revealed similar in situ biaxial response of carotid arteries in OVX and control rats.Conclusions: Carotid artery remodeling in OVX rats was largely consistent with hypertensive remodeling, despite the minor arterial pressure changes found, and was not altered by administration of SID, despite previous evidence of its osteo-protective effect.","PeriodicalId":9167,"journal":{"name":"Biorheology","volume":"54 1","pages":"1-23"},"PeriodicalIF":1.1,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/BIR-16113","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34852165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Impact of training volume and intensity on RBC-NOS/NO pathway and endurance capacity. 训练量和强度对红细胞- nos /NO通路和耐力的影响。

IF 1.1 4区医学

Biorheology Pub Date : 2017-01-01 DOI: 10.3233/BIR-16121

Christina Koliamitra, Bastian Holtkamp, Philipp Zimmer, Wilhelm Bloch, Marijke Grau

{"title":"Impact of training volume and intensity on RBC-NOS/NO pathway and endurance capacity.","authors":"Christina Koliamitra, Bastian Holtkamp, Philipp Zimmer, Wilhelm Bloch, Marijke Grau","doi":"10.3233/BIR-16121","DOIUrl":"https://doi.org/10.3233/BIR-16121","url":null,"abstract":"Background: Acute exercise increases red blood cell-nitric oxide synthase (RBC-NOS) activation and RBC deformability but the effect of regular training remains unclear.Objective: To detect the chronic effect of enduring moderate and high intensity training on the RBC-NOS/NO pathway and to detect a relation between RBC deformability and endurance capacity.Methods: 38 healthy male subjects were randomly assigned to one of three training groups: High Volume Training (HVT; 120-140 beats per minute (bpm)), High Intensity Training (HIT; 160-180 bpm) and Moderate Intensity Training (MIT; 140-160 bpm). Blood parameters, maximum oxygen capacity (VO2 max), RBC deformability, RBC nitrite level and RBC-NOS activation were measured after venous blood sampling at rest pre (T0) and after six weeks of training (T1).Results: RBC-NOS activation, RBC nitrite concentration and RBC deformability were significantly increased at T1 in the HIT group. Parameters were unaltered in MIT and HVT. Maximum oxygen uptake was only significantly increased in the HIT group and regression analysis revealed positive regression between VO2 max and RBC deformability.Conclusions: High intensity training was the only training programme that sustainably affected RBC-NOS dependent NO production and performance capacity. HIT therefore represents a time efficient training program resulting in improved RBC function potentially improving physical condition.","PeriodicalId":9167,"journal":{"name":"Biorheology","volume":"54 1","pages":"37-50"},"PeriodicalIF":1.1,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/BIR-16121","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35159196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Simultaneous assessment of blood coagulation and hematocrit levels in dielectric blood coagulometry. 电介质血液凝固测定中血液凝固和红细胞压积水平的同时评估。

IF 1.1 4区医学

Biorheology Pub Date : 2017-01-01 DOI: 10.3233/BIR-16118

Yoshihito Hayashi, Marc-Aurèle Brun, Kenzo Machida, Seungmin Lee, Aya Murata, Shinji Omori, Hidetoshi Uchiyama, Yoshinori Inoue, Toshifumi Kudo, Takahiro Toyofuku, Masayuki Nagasawa, Isao Uchimura, Tomomasa Nakamura, Takeshi Muneta

{"title":"Simultaneous assessment of blood coagulation and hematocrit levels in dielectric blood coagulometry.","authors":"Yoshihito Hayashi, Marc-Aurèle Brun, Kenzo Machida, Seungmin Lee, Aya Murata, Shinji Omori, Hidetoshi Uchiyama, Yoshinori Inoue, Toshifumi Kudo, Takahiro Toyofuku, Masayuki Nagasawa, Isao Uchimura, Tomomasa Nakamura, Takeshi Muneta","doi":"10.3233/BIR-16118","DOIUrl":"https://doi.org/10.3233/BIR-16118","url":null,"abstract":"Background: In a whole blood coagulation test, the concentration of any in vitro diagnostic agent in plasma is dependent on the hematocrit level but its impact on the test result is unknown.Objective: The aim of this work was to clarify the effects of reagent concentration, particularly Ca2+, and to find a method for hematocrit estimation compatible with the coagulation test.Methods: Whole blood coagulation tests by dielectric blood coagulometry (DBCM) and rotational thromboelastometry were performed with various concentrations of Ca2+ or on samples with different hematocrit levels. DBCM data from a previous clinical study of patients who underwent total knee arthroplasty were re-analyzed.Results: Clear Ca2+ concentration and hematocrit level dependences of the characteristic times of blood coagulation were observed. Rouleau formation made hematocrit estimation difficult in DBCM, but use of permittivity at around 3 MHz made it possible. The re-analyzed clinical data showed a good correlation between permittivity at 3 MHz and hematocrit level (R2=0.83).Conclusions: Changes in the hematocrit level may affect whole blood coagulation tests. DBCM has the potential to overcome this effect with some automated correction using results from simultaneous evaluations of the hematocrit level and blood coagulability.","PeriodicalId":9167,"journal":{"name":"Biorheology","volume":"54 1","pages":"25-35"},"PeriodicalIF":1.1,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/BIR-16118","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35312238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Abstracts of eighth annual meeting, Japanese Society of Biorheology. Tokyo, Japan, 15-16 June 1985. 日本生物流变学会第八届年会摘要。1985年6月15日至16日，日本东京。

IF 1.1 4区医学

Biorheology Pub Date : 2016-12-22 DOI: 10.3233/CH-1985-5522

M. Asano, C. Ohkubo

引用次数: 0

Robert Gwyn Macfarlane's contribution to the organization of biorheology--and other reminiscences. 罗伯特·格温·麦克法兰(Robert Gwyn Macfarlane)对生物流变学组织的贡献——以及其他回忆。

IF 1.1 4区医学

Biorheology Pub Date : 2016-12-09 DOI: 10.3233/CH-1988-8104

A. Copley

引用次数: 0

Biorheology in the practice of medicine: resonance thrombography. 医学实践中的生物流变学:共振血栓造影术。

IF 1.1 4区医学

Biorheology Pub Date : 2016-12-09 DOI: 10.3233/CH-1983-3602

H. Hartert

引用次数: 9

Effect of circulating tissue factor on hypercoagulability in type 2 diabetes mellitus studied by rheometry and dielectric blood coagulometry 采用流变仪和介电凝血法研究循环组织因子对2型糖尿病患者高凝性的影响

IF 1.1 4区医学

Biorheology Pub Date : 2016-11-16 DOI: 10.3233/BIR-16107

I. Uchimura, M. Kaibara, M. Nagasawa, Y. Hayashi

{"title":"Effect of circulating tissue factor on hypercoagulability in type 2 diabetes mellitus studied by rheometry and dielectric blood coagulometry","authors":"I. Uchimura, M. Kaibara, M. Nagasawa, Y. Hayashi","doi":"10.3233/BIR-16107","DOIUrl":"https://doi.org/10.3233/BIR-16107","url":null,"abstract":"Background: Hypercoagulability in type 2 diabetes mellitus (T2DM) patients increases their risk of cardiovascular diseases. Objective: The aim of this work was to investigate the hypercoagulation mechanism in T2DM patients in terms of circulating tissue factor (TF). Methods: Whole blood coagulation tests by damped oscillation rheometry and dielectric blood coagulometry (DBCM) were performed. Results: The average coagulation time was significantly shorter for T2DM patients than for healthy controls. In vitro addition of either anti-TF or anti-activated factor VII (FVIIa) antibody to hypercoagulable blood samples prolonged coagulation times for one group of patients, while coagulation times remained short for another group. The levels of circulating TF were estimated in the former group by measuring the coagulation times for blood samples from healthy subjects with addition of various concentrations of TF and comparing them with the coagulation times for the group. The results indicated that the levels of circulating TF were on the order of subpicomolar at most. Conclusions: Circulating TF is at least partially responsible for a hypercoagulable group of T2DM patients, while an abnormality in the intrinsic coagulation pathway probably occurs in the other group.","PeriodicalId":9167,"journal":{"name":"Biorheology","volume":"53 1","pages":"209 - 219"},"PeriodicalIF":1.1,"publicationDate":"2016-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/BIR-16107","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69793520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

The effect of acute and short term normobaric hyperoxia on hemorheologic parameters. 急性和短期常压高氧对血液流变学参数的影响。

IF 1.1 4区医学

Biorheology Pub Date : 2016-11-09 DOI: 10.3233/BIR-16096

Pinar Ulker

{"title":"The effect of acute and short term normobaric hyperoxia on hemorheologic parameters.","authors":"Pinar Ulker","doi":"10.3233/BIR-16096","DOIUrl":"https://doi.org/10.3233/BIR-16096","url":null,"abstract":"Backround:Possible toxic effects of hyperoxia have been reported previously. However, the number of studies investigating the influence of hyperoxia on blood cells is limited and there are no data regarding its hemorheological effects.Objective: The aim of this study was to investigate the effects of acute hyperoxia, performed in human subjects at normal atmospheric pressure, on the rheological properties of blood.Method: The study was conducted with 12 brain death patients mechanically ventilated in the intensive care unit. The patients were ventilated with 21%, 40%, and 100% oxygen before induction of apnea testing performed for diagnosis of brain death. Blood samples were obtained at each oxygen concentration value for all patients.Result: The results of the study indicated no significant change of red blood cell aggregation, deformability and plasma or whole blood viscosity associated with acute hyperoxia at normobaric conditions.Conclusion: The results of the study suggest that application of normobaric hyperoxia does not have detrimental effects on hemorheological parameters in brain death patients, and that organs considered for donation from such subjects are not adversely affected by abnormalities of blood flow and tissue perfusion.","PeriodicalId":9167,"journal":{"name":"Biorheology","volume":"53 3-4","pages":"171-177"},"PeriodicalIF":1.1,"publicationDate":"2016-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/BIR-16096","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34342110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Large artery biomechanical, geometrical, and structural remodeling elicited by long-term propranolol administration in an animal model. 长期服用心得安引起的动物模型大动脉生物力学、几何和结构重塑。

IF 1.1 4区医学

Biorheology Pub Date : 2016-11-09 DOI: 10.3233/BIR-16090

Konstantinos M Lampropoulos, Dimitrios P Sokolis

{"title":"Large artery biomechanical, geometrical, and structural remodeling elicited by long-term propranolol administration in an animal model.","authors":"Konstantinos M Lampropoulos, Dimitrios P Sokolis","doi":"10.3233/BIR-16090","DOIUrl":"https://doi.org/10.3233/BIR-16090","url":null,"abstract":"Background: Limited attention has been paid to the role of beta-adrenergic blocking agents on large artery function/structure, despite being clinically useful for treating many forms of cardiovascular disease.Objective: To assess long-term consequences of beta-blocker administration on the biomechanical properties, geometry, and histological structure of two major elastic arteries.Methods: Healthy male rats received water with their food, while beta-blockade was produced in rats by adding propranolol in their drinking water. The thoracic aorta and carotid artery were resected after three months for biomechanical (failure and inflation-extension) testing along with geometrical and histological evaluation.Results: The thoracic aorta presented increased strength longitudinally in propranolol-treated than untreated rats, resulting from increased adventitial collagen content. The distensibility of carotid artery increased in propranolol-treated rats at low-to-physiologic pressures, resulting from decreased medial collagen content. Structural remodeling was characterized by reduced lumen diameter, wall mass, and thickness-to-radius ratio. The latter, together with the greater resorption of the media than adventitia, related with the measured opening angle decrease in propranolol-treated rats.Conclusions: The geometrical/biomechanical remodeling was mediated by the hemodynamic effects of propranolol treatment, namely the reduced blood flow, and served to normalize in vivo hoop stresses as well as vessel compliance.","PeriodicalId":9167,"journal":{"name":"Biorheology","volume":"53 3-4","pages":"151-170"},"PeriodicalIF":1.1,"publicationDate":"2016-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/BIR-16090","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34715306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2