Effects of aging and gender on micro-rheology of blood in 3 to 18 months old male and female Wistar (Crl:WI) rats.

IF 1 4区医学 Q4 BIOPHYSICS

Biorheology Pub Date : 2018-01-01 DOI:10.3233/BIR-17148

Viktoria Somogyi, Katalin Peto, Adam Deak, Bence Tanczos, Norbert Nemeth

{"title":"Effects of aging and gender on micro-rheology of blood in 3 to 18 months old male and female Wistar (Crl:WI) rats.","authors":"Viktoria Somogyi, Katalin Peto, Adam Deak, Bence Tanczos, Norbert Nemeth","doi":"10.3233/BIR-17148","DOIUrl":null,"url":null,"abstract":"Background: Age- and gender-related alterations of hemorheological parameters have not been completely elucidated to date. Experiments on older animals may give valuable information on this issue. However, the majority of rheological studies have been performed in young rodents.Objective: We aimed to investigate the influence of aging and gender on hemorheological parameters in rats.Methods: Coeval male (n=10) and female (n=10) Wistar (Crl:WI) rats were followed-up over 15 months. Blood samples were obtained from the lateral tail vein at 3, 4, 5, 9, 12, 15 and 18 months of age. Hematological parameters, red blood cell deformability (elongation under shear), osmotic gradient deformability and erythrocyte aggregation were tested. Body weight and the estrus cycle (in females) were also examined.Results: Erythrocyte aggregation showed age- and gender-related variations. Red blood cell deformability was greater in females and gradually decreased over the 15-month period in both genders. Erythrocyte aggregation was greater in male rats at most ages, but did not show consistent changes with age.Conclusions: The micro-rheological parameters showed age-related alterations with gender differences. The effect of the estrous cycle cannot be excluded in female rats. The results provide reference data for studies of aging in rats and of the mechanism related to age and gender differences in hemorheology.","PeriodicalId":9167,"journal":{"name":"Biorheology","volume":"54 5-6","pages":"127-140"},"PeriodicalIF":1.0000,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/BIR-17148","citationCount":"11","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biorheology","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.3233/BIR-17148","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOPHYSICS","Score":null,"Total":0}

引用次数: 11

Abstract

Background: Age- and gender-related alterations of hemorheological parameters have not been completely elucidated to date. Experiments on older animals may give valuable information on this issue. However, the majority of rheological studies have been performed in young rodents.

Objective: We aimed to investigate the influence of aging and gender on hemorheological parameters in rats.

Methods: Coeval male (n=10) and female (n=10) Wistar (Crl:WI) rats were followed-up over 15 months. Blood samples were obtained from the lateral tail vein at 3, 4, 5, 9, 12, 15 and 18 months of age. Hematological parameters, red blood cell deformability (elongation under shear), osmotic gradient deformability and erythrocyte aggregation were tested. Body weight and the estrus cycle (in females) were also examined.

Results: Erythrocyte aggregation showed age- and gender-related variations. Red blood cell deformability was greater in females and gradually decreased over the 15-month period in both genders. Erythrocyte aggregation was greater in male rats at most ages, but did not show consistent changes with age.

Conclusions: The micro-rheological parameters showed age-related alterations with gender differences. The effect of the estrous cycle cannot be excluded in female rats. The results provide reference data for studies of aging in rats and of the mechanism related to age and gender differences in hemorheology.

查看原文本刊更多论文

年龄和性别对3 ~ 18月龄Wistar雄性和雌性大鼠血液微流变学的影响。

背景:与年龄和性别相关的血液流变学参数的改变至今尚未完全阐明。在老年动物身上进行的实验可能会给这个问题提供有价值的信息。然而，大多数流变学研究都是在幼龄啮齿动物中进行的。目的:探讨年龄和性别对大鼠血液流变学参数的影响。方法:对同年龄Wistar (Crl:WI)雄性大鼠和雌性大鼠进行15个月的随访。分别于3、4、5、9、12、15和18月龄时取尾侧静脉血样。测定血液学参数、红细胞变形能力(剪切伸长率)、渗透梯度变形能力和红细胞聚集性。体重和发情周期(雌性)也进行了检查。结果:红细胞聚集表现出与年龄和性别相关的变化。女性的红细胞变形能力更大，在15个月的时间里，男女的红细胞变形能力都逐渐下降。在大多数年龄雄性大鼠红细胞聚集较大，但不随年龄变化一致。结论:微流变学参数表现出与年龄相关的性别差异。在雌性大鼠中不能排除发情周期的影响。研究结果为大鼠衰老及血液流变学中年龄和性别差异相关机制的研究提供了参考数据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Biorheology 医学-工程：生物医学

CiteScore

2.00

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Biorheology is an international interdisciplinary journal that publishes research on the deformation and flow properties of biological systems or materials. It is the aim of the editors and publishers of Biorheology to bring together contributions from those working in various fields of biorheological research from all over the world. A diverse editorial board with broad international representation provides guidance and expertise in wide-ranging applications of rheological methods to biological systems and materials. The scope of papers solicited by Biorheology extends to systems at different levels of organization that have never been studied before, or, if studied previously, have either never been analyzed in terms of their rheological properties or have not been studied from the point of view of the rheological matching between their structural and functional properties. This biorheological approach applies in particular to molecular studies where changes of physical properties and conformation are investigated without reference to how the process actually takes place, how the forces generated are matched to the properties of the structures and environment concerned, proper time scales, or what structures or strength of structures are required.