Biorheology最新文献

The impact of measurement techniques and common effectors on red blood cell aggregation.

IF 1 4区医学

Biorheology Pub Date : 2024-12-22 DOI: 10.1177/0006355X241297017

Kaitlyn Chien, Honglei Liu, Silvie Suriany, Jon A Detterich

{"title":"The impact of measurement techniques and common effectors on red blood cell aggregation.","authors":"Kaitlyn Chien, Honglei Liu, Silvie Suriany, Jon A Detterich","doi":"10.1177/0006355X241297017","DOIUrl":"https://doi.org/10.1177/0006355X241297017","url":null,"abstract":"Background: Red blood cell aggregation is largely influenced by hematocrit and plasma protein content with aggregation increasing as hematocrit and fibrinogen increase. Aggregation measurement techniques include light-transmission or laser-backscatter, and the impact of measurement technique on aggregation measurements with respect to changes in hematocrit and plasma protein is not well understood.Objective: The objective of this study was to evaluate aggregation measurement techniques with respect to common effectors of red blood cell aggregation.Methods: We obtained whole blood samples from 10 healthy participants and varied hematocrit, plasma albumin, and plasma fibrinogen concentration in a sample of healthy male and female participants. We then measured red blood cell aggregation using a Myrenne Aggregometer (light-transmission aggregometer) and a Laser Optical Rotational Red Cell Analyzer (laser-backscatter aggregometer).Results: For Myrenne, aggregation increased from 30% to 40% hematocrit, then declined at 50% hematocrit. Measurement in the LORRCA demonstrated an increase as hematocrit increased from 30% to 40%, and again increased at 50%, which differed from the light transmission method. Red blood cell aggregation increased with increased fibrinogen concentration, but did not change significantly as albumin concentration increased.Conclusions: Red blood cell aggregation is expected to increase as hematocrit increases, but when light transmission aggregometry is used, aggregation decreases as hematocrit increases past 40%. Each measurement technique has its own set of methodological strengths and weaknesses.","PeriodicalId":9167,"journal":{"name":"Biorheology","volume":" ","pages":"6355X241297017"},"PeriodicalIF":1.0,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143456973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Blood rheology and systemic oxidative status in patients with acromegaly.

IF 1 4区医学

Biorheology Pub Date : 2024-10-29 DOI: 10.1177/0006355X241290980

Emine Kilic-Toprak, Guzin Fidan Yaylali, Yasin Ozdemir, Senay Topsakal, Ozgen Kilic-Erkek, Burak Oymak, Melek Bor-Kucukatay

{"title":"Blood rheology and systemic oxidative status in patients with acromegaly.","authors":"Emine Kilic-Toprak, Guzin Fidan Yaylali, Yasin Ozdemir, Senay Topsakal, Ozgen Kilic-Erkek, Burak Oymak, Melek Bor-Kucukatay","doi":"10.1177/0006355X241290980","DOIUrl":"https://doi.org/10.1177/0006355X241290980","url":null,"abstract":"Background: Cardiovascular system is known to be affected in acromegaly. However, it was not reported to date if hemorheology was also altered and there is limited data regarding systemic oxidative status in patients with acromegaly.Objective: To investigate alterations in hemorheology and systemic oxidative stress in patients with acromegaly.Methods: The study comprised 19 active (AA) and 15 controlled (CA) acromegaly patients (n = 34, mean age 48.85 ± 1.69 years) and 29 controls (mean age 52.83 ± 1.37 years). Erythrocyte aggregation index (AI), aggregation half time (t½), amplitude (Amp) and erythrocyte elongation index (EI) were determined by an ektacytometer. Commercial kits were used for measuring serum total oxidant status (TOS) and total antioxidant status (TAS), and oxidative stress index (OSI) was calculated.Results: There were statistically significant differences regarding EI at shear stresses of 16.87 ve 30.00 Pa (p = 0.034, p = 0.0001, respectively) and AI (p = 0.050, p = 0.035, respectively) values among the control, AA and CA groups. Decrement of t½ was in line with increment of AI and indicated increased erythrocyte aggregation. Serum TOS, TAS and OSI values were similar between the control, AA and CA groups (p > 0.05).Conclusions: This pilot study demonstrated reduced erythrocyte deformability and increased erythrocyte aggregation in patients with AA and CA.","PeriodicalId":9167,"journal":{"name":"Biorheology","volume":" ","pages":"6355X241290980"},"PeriodicalIF":1.0,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143456964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Abstracts of the 2nd Annual Conference of the Austrian Society for Rheology. 奥地利流变学学会第二届年会摘要。

IF 1.1 4区医学

Biorheology Pub Date : 2024-03-12 DOI: 10.3233/BIR-239001

引用次数: 0

Abstracts of the 2nd Annual Conference of the Austrian Society for Rheology. 奥地利流变学学会第二届年会摘要。

IF 1.1 4区医学

Biorheology Pub Date : 2024-03-12 DOI: 10.3233/BIR-239002

引用次数: 0

HIIT serves as an efficient training strategy for basketball players by improving blood fluidity and decreasing oxidative stress 通过改善血液流动性和减少氧化应激，HIIT 成为篮球运动员的高效训练策略

IF 1.1 4区医学

Biorheology Pub Date : 2024-03-05 DOI: 10.3233/bir-230024

Riza Altinel, Ozgen Kilic-Erkek, Emine Kilic-Toprak, Bayram Ozhan, Ahmet Yildirim, Melek Bor-Kucukatay

{"title":"HIIT serves as an efficient training strategy for basketball players by improving blood fluidity and decreasing oxidative stress","authors":"Riza Altinel, Ozgen Kilic-Erkek, Emine Kilic-Toprak, Bayram Ozhan, Ahmet Yildirim, Melek Bor-Kucukatay","doi":"10.3233/bir-230024","DOIUrl":"https://doi.org/10.3233/bir-230024","url":null,"abstract":"BACKGROUND:A challenge for coaches and athletes is to find the best combination of exercises during training. Considering its favorable effects, HIIT has been very popular recently. OBJECTIVE:The goal of this study was to investigate anthropometric features, performance, erythrocyte deformability,plasma viscosity (PV) and oxidative stress in response to acute and long-term (6 weeks) HIIT in adolescent basketball players. METHODS:22 sportsmen between the ages of 14–16 were included. Tabata protocol was applied to the HIIT group in addition to their routine training program 3 days/week, for 6 weeks. Erythrocyte deformability was determined using an ectacytometer (LORCA), PV with a rotational viscometer. Total oxidant status (TOS), total antioxidant status (TAS) were measured by kits. RESULTS:HIIT for 6 weeks induced an improvement in performance tests and waist circumference. 6 weeks of HIIT resulted in a decrement, while the last exercise session yielded an increment in RBC deformability. PV and TOS of HIIT groups were decreased on the 6th week. CONCLUSIONS:Our results demonstrate that, HIIT in addition to the routine exercise program is beneficial for improving performance and blood fluidity as well as decreasing oxidative stress in basketball players. Therefore, HIIT seems as an efficient training strategy for highly-trained individuals.","PeriodicalId":9167,"journal":{"name":"Biorheology","volume":"63 1","pages":""},"PeriodicalIF":1.1,"publicationDate":"2024-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140070283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The rheology of interactions between leukocytes, platelets and the vessel wall in thrombo-inflammation 血栓性炎症中白细胞、血小板和血管壁之间相互作用的流变学原理

IF 1.1 4区医学

Biorheology Pub Date : 2024-02-02 DOI: 10.3233/bir-230040

Gerard B. Nash

{"title":"The rheology of interactions between leukocytes, platelets and the vessel wall in thrombo-inflammation","authors":"Gerard B. Nash","doi":"10.3233/bir-230040","DOIUrl":"https://doi.org/10.3233/bir-230040","url":null,"abstract":"Leukocytes and platelets must adhere to the wall of blood vessels to carry out their protective functions in inflammation and haemostasis. Recruitment is critically dependent on rheological variables (wall shear rate and stress, red cell aggregation and haematocrit) which affect delivery to the vessel wall as well as velocities and forces experienced there. Leukocyte recruitment is efficient only up to wall shear rates of about 300 s−1 and usually restricted to low-shear post-capillary venules in inflammation. Being smaller, platelets experience lower velocities and shear forces adjacent to the wall and can adhere at much higher shear rates for haemostasis in arteries. In addition, we found quite different effects of variations in haematocrit or red cell aggregation on attachment of neutrophils or platelets, which also assist their separate recruitment in venules or arteries. However, it has become increasingly evident that inflammatory and thrombotic responses may occur together, with platelets promoting the adhesion and activation of neutrophils and monocytes. Indeed, it is 30 years since we demonstrated that platelets could cause neutrophils to aggregate in suspension and, when attached to a surface, could support selectin-mediated rolling of all leukocytes. Thrombin-activated platelets could further induce neutrophil activation and immobilisation. In some conditions, platelets could bind to intact endothelial monolayers and capture neutrophils or monocytes. Subsequently, we found that extracellular vesicles released by activated platelets (PEV) fulfilled similar functions when deposited on surfaces or bound to endothelial cells. In murine models, platelets or PEV could act as bridges for monocytes in inflamed vessels. Thus, leukocytes and platelets are rheologically adapted for their separate functions, while novel thrombo-inflammatory pathways using platelets or PEV may underlie pathogenic leukocyte recruitment.","PeriodicalId":9167,"journal":{"name":"Biorheology","volume":"21 1","pages":""},"PeriodicalIF":1.1,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140069867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Poiseuille Gold Medal Awardee 2023. 2023 年 Poiseuille 金奖获得者。

IF 1.1 4区医学

Biorheology Pub Date : 2024-01-01 DOI: 10.3233/BIR-240006

Herbert H Lipowsky, Brian M Cooke

引用次数: 0

Investigation and prediction of the extensional viscosity of okra mucilage using the Giesekus model. 用Giesekus模型研究和预测秋葵黏液的拉伸粘度。

IF 1.1 4区医学

Biorheology Pub Date : 2023-01-01 DOI: 10.3233/BIR-220006

Nguyen Ngoc Minh

{"title":"Investigation and prediction of the extensional viscosity of okra mucilage using the Giesekus model.","authors":"Nguyen Ngoc Minh","doi":"10.3233/BIR-220006","DOIUrl":"https://doi.org/10.3233/BIR-220006","url":null,"abstract":"Background: Okra is a vegetable that is widely grown around the world. Okra mucilage contains a high mucus concentration that can be useful for supporting the swallowing process. Although the extensional rheology of okra mucilage is essential to its flow, its extensional viscosity has not received much attention.Objective: Using a filament stretching rheometer, the extensional viscosity of the mucilage in okra was examined. The Giesekus model was used to predict this parameter.Methods: The okra mucilage with different concentrations was extracted from fresh okra. The extensional viscosity was measured using a filament breakup apparatus. The diameter of the liquid bridge was measured by a laser micrometer and it was also observed by a high-speed camera. A rotational rheometer was used to measure the shear viscosity. In addition, the master curves for the shear viscosity were plotted to eliminate the influence of solvent and shear rate and evaluate the influence of concentration on the elasticity of okra mucilage. The okra mucilage shear and extensional viscosity were predicted using the Giesekus model.Results: Every sample of okra mucilage exhibited shear thinning behavior. In addition to having a high extensional viscosity that is hundreds of times higher than its shear viscosity, okra mucilage also exhibited stretching phenomena. The master curves demonstrated that the pseudoplasticity of the okra mucilage increased along with the concentration. The rheological behavior of the mucilage in okra can be explained by the Giesekus model.Conclusions: Okra mucilage's shear viscosity exhibited shear thinning behavior and a strong extensional viscosity that was significantly higher than its shear viscosity. The shear and extensional viscosity of okra mucilage can be described and predicted using the Giesekus model.","PeriodicalId":9167,"journal":{"name":"Biorheology","volume":"59 1-2","pages":"29-42"},"PeriodicalIF":1.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9594607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rheology of saliva in health and disease. 唾液流变学在健康和疾病中的应用。

IF 1.1 4区医学

Biorheology Pub Date : 2023-01-01 DOI: 10.3233/BIR-210014

S K Rereddy, A C Cao, B Blackwell, R Poling-Skutvik, P E Arratia, N Mirza

{"title":"Rheology of saliva in health and disease.","authors":"S K Rereddy, A C Cao, B Blackwell, R Poling-Skutvik, P E Arratia, N Mirza","doi":"10.3233/BIR-210014","DOIUrl":"https://doi.org/10.3233/BIR-210014","url":null,"abstract":"Background: Saliva is a complex fluid that lubricates the oropharynx and facilitates chewing, swallowing, and vocalization. Viscoelasticity is critical for the ability of saliva to fulfill these functions. Xerostomia, or a sensation of dry mouth, occurs in 17-26% of the population. Although many equate xerostomia with hyposalivation, high-risk patients frequently report oral dryness in the absence of decreased salivary flow.Objective: This study aims to determine if xerostomia is associated with alterations in the rheological properties of saliva in addition to decreased salivary production.Methods: The study population included patients with post-radiation xerostomia, patients with anticholinergic-induced xerostomia and healthy controls. Salivary volumetric flow rate was measured, shear viscosity was measured using oscillatory rheometry, and extensional viscosity was measured using capillary thinning methods. Groups were compared using descriptive statistics and univariate analysis.Results: A total of 36 subjects were included: 15 with post-radiation xerostomia, 9 with anticholinergic-induced xerostomia and 12 controls. Salivary volumetric flow was significantly decreased in post-radiation and anticholinergic-induced patients compared to controls. On capillary thinning testing, saliva from xerostomia patients had significantly greater extensional viscosity compared to controls. However, saliva from the three groups showed no significant difference in the complex viscosity or the storage or loss modulus of saliva with oscillatory rheology.Conclusions: Xerostomia is associated with decreased salivary volumetric flow and quantitative changes in the rheologic properties of saliva.","PeriodicalId":9167,"journal":{"name":"Biorheology","volume":"59 1-2","pages":"19-27"},"PeriodicalIF":1.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9606211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Shear stimulated red blood cell microparticles: Effect on clot structure, flow and fibrinolysis. 剪切刺激红细胞微粒：对血凝块结构、流动和纤维蛋白溶解的影响

IF 1.1 4区医学

Biorheology Pub Date : 2023-01-01 DOI: 10.3233/BIR-220012

James P Buerck, Kylie M Foster, Preston R Larson, Edgar A O'Rear

{"title":"Shear stimulated red blood cell microparticles: Effect on clot structure, flow and fibrinolysis.","authors":"James P Buerck, Kylie M Foster, Preston R Larson, Edgar A O'Rear","doi":"10.3233/BIR-220012","DOIUrl":"10.3233/BIR-220012","url":null,"abstract":"Background: Microparticles (MPs) have activity in thrombus promotion and generation. Erythrocyte microparticles (ErMPs) have been reported to accelerate fibrinolysis in the absence of permeation. We hypothesized that shear induced ErMPs would affect fibrin structure of clots and change flow with implications for fibrinolysis.Objective: To determine the effect of ErMPs on clot structure and fibrinolysis.Methods: Plasma with elevated ErMPs was isolated from whole blood or from washed red blood cells (RBCs) resuspended in platelet free plasma (PFP) after high shear. Dynamic light scattering (DLS) provided size distribution of ErMPs from sheared samples and unsheared PFP controls. Clots were formed by recalcification for flow/lysis experiments and examined by confocal microscopy and SEM. Flow rates through clots and time-to-lysis were recorded. A cellular automata model showed the effect of ErMPs on fibrin polymerization and clot structure.Results: Coverage of fibrin increased by 41% in clots formed from plasma of sheared RBCs in PFP over controls. Flow rate decreased by 46.7% under a pressure gradient of 10 mmHg/cm with reduction in time to lysis from 5.7 ± 0.7 min to 12.2 ± 1.1 min (p < 0.01). Particle size of ErMPs from sheared samples (200 nm) was comparable to endogenous microparticles.Conclusions: ErMPs alter the fibrin network in a thrombus and affect hydraulic permeability resulting in decelerated delivery of fibrinolytic drugs.","PeriodicalId":9167,"journal":{"name":"Biorheology","volume":"59 1-2","pages":"43-59"},"PeriodicalIF":1.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9596205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0