Measurement of adhesive strength between the epidermal and inner tissues of plant stems using a tensile tester.

IF 1 4区医学 Q4 BIOPHYSICS

Biorheology Pub Date : 2025-05-01 Epub Date: 2025-02-21 DOI:10.1177/0006355X241296333

Yuma Shimizu, Kazuyuki Wakabayashi, Kensuke Miyamoto, Kouichi Soga

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引用次数: 0

Abstract

BackgroundThe plant stem is composed of epidermal and inner tissues that are under tension and compression, respectively. Therefore, the adhesion of both tissues is considered to be involved in the structural integrity of the stem. However, the role of tissue adhesion in stem structure is unclear.ObjectiveThis study aimed to develop a method for quantitatively measuring the adhesive strength between the epidermal and inner tissues using a tensile tester to determine the possible role of tissue adhesion in stem integrity.MethodsThe epidermal tissue was partially peeled from the segment of pea epicotyls using forceps to create a peeling arm. The peeling arm and the segment region of the partially removed epidermal tissue were fixed to the upper and lower clamps, respectively. By raising the upper clamp at various speeds, the epidermal tissue was peeled from the segment, and the peeling force was recorded.ResultsAdhesive strength was defined as the peeling force normalized by the width of the peeled epidermal tissue. The peeling rate was determined as 100 mm/min. The adhesive strength in the elongation region of the stem was substantially smaller than in the non-elongation region.ConclusionsA method for quantitatively measuring the adhesive strength between the epidermal and inner tissues was developed. Analysis using this method suggests that adhesive strength may be involved in regulating stem growth.

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用拉力试验机测定植物茎的表皮和内部组织之间的粘接强度。

植物茎由表皮和内部组织组成，它们分别处于拉伸和压缩状态。因此，两种组织的粘连被认为与茎的结构完整性有关。然而，组织粘连在茎结构中的作用尚不清楚。目的建立一种利用拉力仪定量测定茎表皮与内部组织黏附强度的方法，以确定组织黏附在茎完整性中的可能作用。方法用镊子对豌豆上胚轴部分表皮组织进行脱皮，形成脱皮臂。分别将剥离臂和部分剥离表皮组织的节段区域固定在上下钳上。以不同的速度提起上钳，将表皮组织从节段上剥离，并记录剥离力。结果粘附强度定义为剥落力以剥落表皮组织的宽度归一化。剥皮速率确定为100mm /min。茎伸长区域的粘接强度明显小于非伸长区域。结论建立了一种定量测定表皮与内部组织间黏附强度的方法。用这种方法分析表明，粘附强度可能参与调节茎的生长。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biorheology 医学-工程：生物医学

CiteScore

2.00

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Biorheology is an international interdisciplinary journal that publishes research on the deformation and flow properties of biological systems or materials. It is the aim of the editors and publishers of Biorheology to bring together contributions from those working in various fields of biorheological research from all over the world. A diverse editorial board with broad international representation provides guidance and expertise in wide-ranging applications of rheological methods to biological systems and materials. The scope of papers solicited by Biorheology extends to systems at different levels of organization that have never been studied before, or, if studied previously, have either never been analyzed in terms of their rheological properties or have not been studied from the point of view of the rheological matching between their structural and functional properties. This biorheological approach applies in particular to molecular studies where changes of physical properties and conformation are investigated without reference to how the process actually takes place, how the forces generated are matched to the properties of the structures and environment concerned, proper time scales, or what structures or strength of structures are required.