Biorheology最新文献_第8页

Osteoarthritic synovial fluid rheology and correlations with protein concentration. 骨关节炎滑液流变学及其与蛋白浓度的相关性。

IF 1.1 4区医学

Biorheology Pub Date : 2016-11-09 DOI: 10.3233/BIR-15078

A. Madkhali, Michael Chernos, D. Grecov, E. Kwok

{"title":"Osteoarthritic synovial fluid rheology and correlations with protein concentration.","authors":"A. Madkhali, Michael Chernos, D. Grecov, E. Kwok","doi":"10.3233/BIR-15078","DOIUrl":"https://doi.org/10.3233/BIR-15078","url":null,"abstract":"BACKGROUND\u0000Osteoarthritis is a common, localized joint disease that causes pain, stiffness and reduced mobility. Osteoarthritis is particularly common in the knees. The effects of osteoarthritis on the rheology of synovial fluid in the knees are not fully understood and consequently require further study.\u0000\u0000\u0000OBJECTIVE\u0000The purpose of this study is to investigate the effects of protein content on synovial fluid shear rheology. A secondary study outcome will include study of the temperature dependence of synovial fluid behaviour.\u0000\u0000\u0000METHODS\u000038 osteoarthritic synovial fluid samples were studied under shear flow. Shear properties were correlated with protein concentration. Viscosupplement was used as a comparison and to verify measurement reliability. The effects of temperature were investigated at 20, 29 and 37°C.\u0000\u0000\u0000RESULTS\u0000Shear rheological properties were found to vary widely between samples, however all samples demonstrated clear non-Newtonian shear thinning behaviour. In general viscoelastic properties were lower in osteoarthritic samples than previously studied healthy synovial fluid. A moderate correlation was observed between synovial fluid dynamic moduli at a frequency of 2.5 Hz and protein concentration. Temperature was found to affect the rheology of osteoarthritic synovial fluid and was fitted with the Arrhenius model.\u0000\u0000\u0000CONCLUSIONS\u0000Increased protein concentration has been correlated with decreased shear rheological parameters. Temperature dependence of synovial fluid was also demonstrated and modelled for use in Part 2 of this article.","PeriodicalId":9167,"journal":{"name":"Biorheology","volume":"12 1","pages":"111-122"},"PeriodicalIF":1.1,"publicationDate":"2016-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/BIR-15078","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69792754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Rheological effects of macromolecular interactions in synovial fluid. 滑液中大分子相互作用的流变效应。

IF 1.1 4区医学

Biorheology Pub Date : 2016-07-29 DOI: 10.3233/BIR-15104

L Martin-Alarcon, T A Schmidt

{"title":"Rheological effects of macromolecular interactions in synovial fluid.","authors":"L Martin-Alarcon, T A Schmidt","doi":"10.3233/BIR-15104","DOIUrl":"https://doi.org/10.3233/BIR-15104","url":null,"abstract":"The rheological properties of synovial fluid (SF) are largely attributed to the presence of high molecular weight hyaluronan (HA). However, rheological differences between SF and pure HA solutions suggest that SF proteins actively contribute towards the bulk viscoelasticity of this biological fluid. Due to various experimental challenges involved with the rheometry of low-viscosity biological fluids, the macromolecular interactions in SF and their relative rheological importance are still a matter of active discussion. Interestingly however, recent evidence suggests that the concentration and structure of proteoglycan 4 (PRG4, also known as lubricin) can directly modulate the viscoelastic properties of HA-PRG4 solutions. The objective of this review is to highlight recent rheological studies that examine the macromolecular interactions between HA and proteins in SF. First, a general overview of the chemical composition of SF and the molecular structure of its key constituents HA and PRG4 is provided. Subsequently, diverse rheological experimental techniques that have been developed to characterize HA solutions are discussed. Finally, rheological investigations of macromolecular interactions between HA, serum proteins, and PRG4 are examined. This review illustrates how diverse rheological techniques can expand our understanding of the composition-structure-function relationships in SF. ","PeriodicalId":9167,"journal":{"name":"Biorheology","volume":"53 2","pages":"49-67"},"PeriodicalIF":1.1,"publicationDate":"2016-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/BIR-15104","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34326113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 35

Heterogeneous phase fibrinolysis rates by damped oscillation rheometry. 阻尼振荡流变法测定非均相纤维蛋白溶解率。

IF 1.1 4区医学

Biorheology Pub Date : 2016-07-29 DOI: 10.3233/BIR-15089

Jae-Suk Lee, Makoto Kaibara, Edgar A O'Rear

引用次数: 0

Relationship between arterial blood pressure and blood viscosity in spontaneously hypertensive rats treated with pentoxifylline. 己酮茶碱对自发性高血压大鼠动脉血压和血液粘度的影响。

IF 1.1 4区医学

Biorheology Pub Date : 2016-07-26 DOI: 10.3233/BIR-15100

Mark B Plotnikov, Oleg I Aliev, Alexey V Nosarev, Alexander Y Shamanaev, Anastasia V Sidekhmenova, Yana Anfinogenova, Anna M Anishchenko, Ekaterina V Pushkina

{"title":"Relationship between arterial blood pressure and blood viscosity in spontaneously hypertensive rats treated with pentoxifylline.","authors":"Mark B Plotnikov, Oleg I Aliev, Alexey V Nosarev, Alexander Y Shamanaev, Anastasia V Sidekhmenova, Yana Anfinogenova, Anna M Anishchenko, Ekaterina V Pushkina","doi":"10.3233/BIR-15100","DOIUrl":"https://doi.org/10.3233/BIR-15100","url":null,"abstract":"Background: Systemic arterial pressure (AP) depends on two physiological variables: cardiac output (CO) and total peripheral resistance (TPR). The latter depends on vascular hindrance and blood viscosity (BV). However, the relative contributions of the vascular and rheological factors to TPR remain unclear.Objective: The aim of our work was to study the haemodynamic and haemorheologic effects of a treatment course with pentoxifylline (PTX) in SHRs in an effort to assess the impact of the rheological factor on TPR and AP.Methods: The effects of the treatment course with PTX (100 mg/kg/day p.o. for six weeks) on BV, plasma viscosity, haematocrit, erythrocyte aggregation and deformability, mean AP (MAP), stroke volume (SV), CO, and TPR were studied in SHRs and in control Wistar Kyoto (WKY) rats.Results: PTX-treated SHRs had a lower BV, lower erythrocyte aggregation, and higher erythrocyte deformability index compared with the controls. The TPR level was higher by 43% compared with that in WKY rats and did not differ from the values obtained from control SHRs. In SHRs, moderate and strong positive correlations were found between BV and MAP and between BV and TPR. PTX-treated SHRs did not have any significant correlations between the above mentioned parameters.Conclusions: Treatment with PTX attenuated whole blood viscosity, but did not affect the AP and hemodynamic parameters in the experimental SHRs compared with the control SHRs. The magnitude of the rheologic effects of PTX was insufficient to cause appreciable decreases in TPR and AP.","PeriodicalId":9167,"journal":{"name":"Biorheology","volume":"53 2","pages":"93-107"},"PeriodicalIF":1.1,"publicationDate":"2016-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/BIR-15100","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34326111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Atrial natriuretic peptide down-regulates neutrophil recruitment on inflamed endothelium by reducing cell deformability and resistance to detachment force. 心房利钠肽通过降低细胞的变形能力和对脱离力的抵抗，下调炎症内皮的中性粒细胞募集。

IF 1.1 4区医学

Biorheology Pub Date : 2016-07-04 DOI: 10.3233/BIR-15067

V. Morikis, Chris Radecke, Yanyan Jiang, V. Heinrich, F. Curry, S. Simon

{"title":"Atrial natriuretic peptide down-regulates neutrophil recruitment on inflamed endothelium by reducing cell deformability and resistance to detachment force.","authors":"V. Morikis, Chris Radecke, Yanyan Jiang, V. Heinrich, F. Curry, S. Simon","doi":"10.3233/BIR-15067","DOIUrl":"https://doi.org/10.3233/BIR-15067","url":null,"abstract":"BACKGROUND Recombinant atrial natriuretic peptide (ANP) is administered in patients with acute heart failure in Japan to improve renal function and hemodynamics, but its anti-inflammatory effect on activated leukocytes may also contribute to its therapeutic efficacy. OBJECTIVE Examine unconventional role of ANP in neutrophil adhesion to inflamed endothelium. METHODS Human neutrophils were perfused over endothelial monolayers in a microfluidic lab-chip assay. Cell rheology was assessed by micropipette aspiration to assess changes in cortical tension and viscosity. Fluorescence microscopy was applied to measure adhesive contact area and β2-integrin focal bond formation. RESULTS ANP inhibited neutrophil rolling and firm adhesion without influencing the upregulation of cellular adhesion molecules on endothelium or the regulation of high affinity CD18 and shedding of L-selectin during neutrophil activation. Exposed to fluid shear, integrin mediated arrest was disrupted with ANP treatment, which elicited formation of long tethers and diminished cell spreading and contact. This correlated with a ∼40% increase in neutrophil viscosity and a reduction in the adhesive footprint. CONCLUSIONS A decrease in cell deformation and neutrophil flattening with ANP results in fewer integrin bond clusters, which translates to higher tensile forces and impaired adhesion strengthening and cell detachment.","PeriodicalId":9167,"journal":{"name":"Biorheology","volume":"52 5-6 1","pages":"447-63"},"PeriodicalIF":1.1,"publicationDate":"2016-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/BIR-15067","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69793079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Atrial natriuretic peptide down-regulates neutrophil recruitment on inflamed endothelium by reducing cell deformability and resistance to detachment force. 心房利钠肽通过降低细胞的变形能力和对脱离力的抵抗，下调炎症内皮的中性粒细胞募集。

IF 1.1 4区医学

Biorheology Pub Date : 2016-07-04 DOI: 10.3233/BIR-15067A

Vasilios A Morikis, Chris Radecke, Yanyan Jiang, Volkmar Heinrich, Fitz-Roy Curry, Scott I Simon

{"title":"Atrial natriuretic peptide down-regulates neutrophil recruitment on inflamed endothelium by reducing cell deformability and resistance to detachment force.","authors":"Vasilios A Morikis, Chris Radecke, Yanyan Jiang, Volkmar Heinrich, Fitz-Roy Curry, Scott I Simon","doi":"10.3233/BIR-15067A","DOIUrl":"https://doi.org/10.3233/BIR-15067A","url":null,"abstract":"2 BACKGROUND: In Japan, Recombinant atrial natriuretic peptide (ANP) is administered in 3 patients with acute heart failure in Japan to improve renal function and hemodynamics, but its anti-inflammatory effect on activated leukocytes may also contribute to its therapeutic efficacy OBJECTIVE: Examine unconventional role of ANP in neutrophil adhesion to inflamed endothelium. METHODS: Human neutrophils were perfused over endothelial monolayers in a microfluidic 10 lab-chip assay. Cell rheology was assessed by micropipette aspiration to assess changes in 11 cortical tension and viscosity. Fluorescence microscopy was applied to measure adhesive contact 12 area and β 2 -integrin focal bond formation. 13 14 RESULTS: ANP inhibited neutrophil rolling and firm adhesion without influencing the 15 upregulation of cellular adhesion molecules on endothelium or the regulation of high affinity 16 CD18 and shedding of L-selectin during neutrophil activation. Conversion Exposed to fluid 17 shear, to shear resistant integrin mediated arrest was disrupted with ANP treatment, which 18 elicited formation of long tethers and diminished cell spreading and contact. This correlated with 19 an ~40% increase in neutrophil viscosity and a reduction in the adhesive footprint. CONCLUSIONS: A decrease in cell deformation and PMN neutrophil flattening with ANP 22 results in fewer integrin bond clusters, which translates to higher tensile forces and impaired 23 adhesion strengthening and cell detachment. prior to measurement. ANP did not alter the up-regulation of β 2 -integrin or 21 the shedding of L-selectin when compared to untreated control (n=3). PMN F-actin was measured using Phalloidin with and without IL-8 stimulation after simultaneous fixing and 23 permeabilization. Data shows replicates for n=3 donors with no significant effect of ANP 24 observed.","PeriodicalId":9167,"journal":{"name":"Biorheology","volume":"53 2","pages":"109"},"PeriodicalIF":1.1,"publicationDate":"2016-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/BIR-15067A","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34713796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optical twisting to monitor the rheology of single cells. 光学扭曲监测单个细胞的流变。

IF 1.1 4区医学

Biorheology Pub Date : 2016-05-26 DOI: 10.3233/BIR-15084

Matthieu Robert de Saint Vincent

引用次数: 1

Effects of vessel size, cell sedimentation and haematocrit on the adhesion of leukocytes and platelets from flowing blood. 血管大小、细胞沉降和红细胞压积对血液中白细胞和血小板粘附的影响。

IF 1.1 4区医学

Biorheology Pub Date : 2016-02-10 DOI: 10.3233/BIR-15043

Tim Watts, M. Barigou, G. Nash

{"title":"Effects of vessel size, cell sedimentation and haematocrit on the adhesion of leukocytes and platelets from flowing blood.","authors":"Tim Watts, M. Barigou, G. Nash","doi":"10.3233/BIR-15043","DOIUrl":"https://doi.org/10.3233/BIR-15043","url":null,"abstract":"BACKGROUND\u0000Leukocytes and platelets typically fulfil their functions through adhesion to the walls of vessels with different size, haematocrit and shear rate.\u0000\u0000\u0000OBJECTIVE\u0000We aimed to investigate differential effects of these variables on leukocyte and platelet adhesion.\u0000\u0000\u0000METHODS\u0000Blood with varying haematocrit was perfused at a range of wall shear rates through capillaries of depth 100 or 300 µm coated with P-selectin or collagen.\u0000\u0000\u0000RESULTS\u0000Adhesion of leukocytes was much more efficient in the smaller capillaries, but was equal on the upper and lower surfaces and showed nearly identical shear rate dependence for either size of vessel. Platelets also adhered more efficiently in the smaller vessels (although the effect of size was not so great), and equally on upper and lower surfaces, but their adhesion was much less sensitive to increasing shear rate. In previous studies using vertically-orientated capillaries, leukocyte adhesion increased with increasing haematocrit (Am. J. Physiol.285 (2003), H229-H240). Here, in horizontal 100 µm capillaries, leukocyte adhesion was highly efficient at haematocrit of 10% but restricted to the lower surface. Adhesion decreased initially as haematocrit was increased to 30% and then increased slightly again at 40% haematocrit. Increasing haematocrit supported a monotonic increase in platelet adhesion in the horizontal capillaries.\u0000\u0000\u0000CONCLUSIONS\u0000Platelets adhere efficiently over a wider range of sizes and shear rates, and at high haematocrit. Leukocytes adhere better in smaller vessels and at low haematocrit in horizontal vessels. The different behaviours may represent 'rheological adaptation' to functions in inflammation vs. haemostasis.","PeriodicalId":9167,"journal":{"name":"Biorheology","volume":"52 5-6 1","pages":"391-404"},"PeriodicalIF":1.1,"publicationDate":"2016-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/BIR-15043","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69792089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Proteolytic receptor cleavage in the pathogenesis of blood rheology and co-morbidities in metabolic syndrome. Early forms of autodigestion. 蛋白水解受体裂解在血液流变学的发病机制和代谢综合征的合并症。早期形式的自体消化。

IF 1.1 4区医学

Biorheology Pub Date : 2016-02-10 DOI: 10.3233/BIR-15045

R. Mazor, G. Schmid-Schönbein

{"title":"Proteolytic receptor cleavage in the pathogenesis of blood rheology and co-morbidities in metabolic syndrome. Early forms of autodigestion.","authors":"R. Mazor, G. Schmid-Schönbein","doi":"10.3233/BIR-15045","DOIUrl":"https://doi.org/10.3233/BIR-15045","url":null,"abstract":"Abnormal blood rheological properties seldom occur in isolation and instead are accompanied by other complications, often designated as co-morbidities. In the metabolic syndrome with complications like hypertension, diabetes and lack of normal microvascular blood flow, the underlying molecular mechanisms that simultaneously lead to elevated blood pressure and diabetes as well as abnormal microvascular rheology and other cell dysfunctions have remained largely unknown. In this review, we propose a new hypothesis for the origin of abnormal cell functions as well as multiple co-morbidities. Utilizing experimental models for the metabolic disease with diverse co-morbidities we summarize evidence for the presence of an uncontrolled extracellular proteolytic activity that causes ectodomain receptor cleavage and loss of their associated cell function. We summarize evidence for unchecked degrading proteinase activity, e.g. due to matrix metalloproteases, in patients with hypertension, Type II diabetes and obesity, in addition to evidence for receptor cleavage in the form of receptor fragments and decreased extracellular membrane expression levels. The evidence suggest that a shift in blood rheological properties and other co-morbidities may in fact be derived from a common mechanism that is due to uncontrolled proteolytic activity, i.e. an early form of autodigestion. Identification of the particular proteases involved and the mechanisms of their activation may open the door to treatment that simultaneously targets multiple co-morbidities in the metabolic syndrome.","PeriodicalId":9167,"journal":{"name":"Biorheology","volume":"149 1","pages":"337-52"},"PeriodicalIF":1.1,"publicationDate":"2016-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/BIR-15045","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69792184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Laudatio for Harry Goldsmith. 为哈里·戈德史密斯鼓掌。

IF 1.1 4区医学

Biorheology Pub Date : 2016-02-10 DOI: 10.3233/BIR-150676

G. Cokelet

引用次数: 0