Bosnian journal of basic medical sciences最新文献_第5页

MicroRNA-573 inhibits cell proliferation, migration and invasion and is downregulated by PICSAR in cutaneous squamous cell carcinoma. MicroRNA-573在皮肤鳞状细胞癌中抑制细胞增殖、迁移和侵袭，并被PICSAR下调。

IF 3.4 4区医学

Bosnian journal of basic medical sciences Pub Date : 2022-04-01 DOI: 10.17305/bjbms.2021.6301

Yanhua Wang, Shengjian Tang, Jianping Lv

{"title":"MicroRNA-573 inhibits cell proliferation, migration and invasion and is downregulated by PICSAR in cutaneous squamous cell carcinoma.","authors":"Yanhua Wang, Shengjian Tang, Jianping Lv","doi":"10.17305/bjbms.2021.6301","DOIUrl":"https://doi.org/10.17305/bjbms.2021.6301","url":null,"abstract":"The incidence of cutaneous squamous cell carcinoma (cSCC) has been increasing in recent years. Meanwhile, microRNAs (miRNAs) have been found to play vital roles in various cancers, including cSCC. This study aimed to investigate the expression of microRNA-573 (miR-573) in cSCC, its relationship with long non-coding RNA PICSAR and analyze its biological role. The relationship between PICSAR and miR-573 was confirmed by dual-luciferase reporter assay and Pearson's correlation coefficient analysis. The levels of PICSAR and miR-573 were measured using quantitative Real-Time PCR. Cell Counting Kit-8 assay was used to evaluate the cSCC cell proliferation ability. The migration and invasion abilities of cSCC cells were evaluated by Transwell assay. PICSAR expression was increased and miR-573 was decreased in tumor tissues and cSCC cell lines. PICSAR and miR-573 can bind directly, and miR-573 expression was downregulated by PICSAR in cSCC. Overexpression of miR-573 significantly inhibited the proliferation, migration and invasion abilities of A431 and SCC13 cells. Additionally, miR-573 overexpression reversed the promotion effects of PICSAR overexpression on cSCC cell proliferation, migration and invasion abilities. In conclusion, our findings indicated that miR-573 expression was decreased in tumor tissues and cSCC cells and was downregulated by PICSAR in cSCC. Additionally, miR-573 overexpression inhibited cSCC cell proliferation, migration and invasion, and reversed the promotion effects of PICSAR overexpression on cSCC cell biological functions. Thus, miR-573 might function as a tumor suppressor and might be involved in the regulatory effects of PICSAR on tumorigenesis in cSCC.","PeriodicalId":9147,"journal":{"name":"Bosnian journal of basic medical sciences","volume":"22 2","pages":"229-237"},"PeriodicalIF":3.4,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8977082/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39601082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Role of dynamic contrast enhanced magnetic resonance imaging in the diagnosis and management of vascular lesions of the head and neck. 动态增强磁共振成像在头颈部血管病变的诊断和治疗中的作用。

IF 3.4 4区医学

Bosnian journal of basic medical sciences Pub Date : 2022-04-01 DOI: 10.17305/bjbms.2021.6019

Raluca Petea-Balea, Manuela Lenghel, Horatiu Rotar, Cristian Dinu, Simion Bran, Florin Onisor, Rares Roman, Simona Senila, Csaba Csutak, Anca Ciurea

{"title":"Role of dynamic contrast enhanced magnetic resonance imaging in the diagnosis and management of vascular lesions of the head and neck.","authors":"Raluca Petea-Balea, Manuela Lenghel, Horatiu Rotar, Cristian Dinu, Simion Bran, Florin Onisor, Rares Roman, Simona Senila, Csaba Csutak, Anca Ciurea","doi":"10.17305/bjbms.2021.6019","DOIUrl":"https://doi.org/10.17305/bjbms.2021.6019","url":null,"abstract":"Vascular anomalies comprise a wide and heterogeneous group of lesions that may be found in all parts of the body, with most of the cases of vascular malformations involving the head and neck region. Ultrasound (US) is the reliable first-line imaging technique to assess flow parameters. However, in some cases, US fails to depict the real extent of the lesions. On the other hand, magnetic resonance imaging (MRI) allows the evaluation of the full extension and anatomic relationship of the vascular anomalies with the neighboring structures and provides hemodynamic characterization using dynamic contrast enhanced MRI (DCE-MRI), avoiding unnecessary invasive catheter-based procedures. DCE-MRI angiography can make a distinction between low and high flow vascular anomalies and it is useful for selecting adequate therapy and appreciating prognosis. The aim of this paper is to review the role of DCE -MRI in the evaluation of flow characteristics and lesion extent in vascular anomalies of the head and neck region.","PeriodicalId":9147,"journal":{"name":"Bosnian journal of basic medical sciences","volume":"22 2","pages":"156-163"},"PeriodicalIF":3.4,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8977080/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39333958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Inflammatory cells in the ascending aortic aneurysm in patients with type 2 diabetes versus patients with hypertension. 2型糖尿病患者与高血压患者升主动脉瘤内炎症细胞的比较

IF 3.4 4区医学

Bosnian journal of basic medical sciences Pub Date : 2022-04-01 DOI: 10.17305/bjbms.2021.6488

Aleksandra Milutinović, Ruda Zorc-Pleskovič

{"title":"Inflammatory cells in the ascending aortic aneurysm in patients with type 2 diabetes versus patients with hypertension.","authors":"Aleksandra Milutinović, Ruda Zorc-Pleskovič","doi":"10.17305/bjbms.2021.6488","DOIUrl":"https://doi.org/10.17305/bjbms.2021.6488","url":null,"abstract":"Aortic aneurysms occur relatively frequently in the ascending thoracic aorta, but are rarely seen in patients with type 2 diabetes. Our aim was to evaluate inflammatory cell infiltration in the ascending aortic aneurysm wall in patients with diabetes without arterial hypertension (DM2 group, N=6) versus hypertensive non-diabetic patients (AH group, N=34). For histologic analysis, the sections were stained with hematoxylin-eosin and Movat pentachrome. The immunohistochemical staining was used to analyze the infiltration of pro-inflammatory (CD68) and anti-inflammatory macrophages (CD163), T helper (CD4) and T killer cells (CD8), and B (CD79a) and plasma cells (CD138) in all three layers of aneurysms of both groups. The statistical significance of the differences between groups was evaluated by ANOVA and the Welch test. In comparison to the AH group, the DM2 group developed less severe infiltration of pro-inflammatory macrophages (P=0.004) and B cells (P=0.025) in the tunica intima, and tunica media (P=0.049, P=0.007, respectively), and fewer plasma cells in the tunica media (P=0.024) and tunica adventitia (P=0.017). We found no significant differences in the number of T helper, T killer cells, and anti-inflammatory macrophages and in the amount of collagen and elastic fibers, ground substance, and smooth muscle cells in all three layers of the vessel wall. Except in tunica adventitia of DM2 group, there were more collagen fibers overall (P=0.025). Thus, we conclude that the histological structure of the aneurysm in diabetics without hypertension is almost the same as in hypertensive patients without diabetes. Diabetics had significantly less inflammatory infiltration in all three layers of the vessel wall, and more collagen fibers in tunica adventitia.","PeriodicalId":9147,"journal":{"name":"Bosnian journal of basic medical sciences","volume":"22 2","pages":"178-184"},"PeriodicalIF":3.4,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8977081/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39525936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Poorly differentiated clusters and tumor budding are important prognostic factors in colorectal carcinomas. 低分化簇和肿瘤出芽是结直肠癌重要的预后因素。

IF 3.4 4区医学

Bosnian journal of basic medical sciences Pub Date : 2022-04-01 DOI: 10.17305/bjbms.2021.6110

Aura Jurescu, Alis Dema, Adrian Văduva, Adelina Gheju, Octavia Vița, Robert Barna, Codruța Lăzureanu, Marioara Cornianu, Sorina Tăban, Ciprian Duță, Stelian Pantea

{"title":"Poorly differentiated clusters and tumor budding are important prognostic factors in colorectal carcinomas.","authors":"Aura Jurescu, Alis Dema, Adrian Văduva, Adelina Gheju, Octavia Vița, Robert Barna, Codruța Lăzureanu, Marioara Cornianu, Sorina Tăban, Ciprian Duță, Stelian Pantea","doi":"10.17305/bjbms.2021.6110","DOIUrl":"https://doi.org/10.17305/bjbms.2021.6110","url":null,"abstract":"The aim of our study was to assess the prognostic value of the two new grading systems based on the quantification of tumor budding - TB (GBd) and poorly differentiated clusters - PDCs (PDCs-G) in colorectal carcinomas (CRC). We performed a retrospective study on 71 CRC patients who underwent surgery at the Emergency County Hospital, Timișoara. CRC cases were classified based on haematoxylin-eosin slides, using the conventional grading system, GBd and PDCs-G, respectively. We used two-tier and three-tier grading schemes for each system. Subsequently, we evaluated associations with other prognostic factors in CRC. Based on the three-tier GBd (GBd-3t) most cases (34/69, 49.27%) were classified as G3Bd-3t, while based on the conventional grading system, the majority of the cases (55/69, 79.71%) were considered G2. On the other hand, based on the three-tier PDCs-G system (PDCs-G-3t), most cases (31/69, 44.93%) were PDCs-G2-3t. We also noted a more significant association of GBd-3t with other prognostic parameters analyzed, as compared to the conventional grading system. Nodal status, tumor stage, and lymphovascular invasion were strongly correlated with GBd-3t (p=0.0001). Furthermore, we noted that PDCs-G-3t correlated more significantly than the conventional grading system with nodal status (p<0.0001), tumor stage (p=0.0003), lymphovascular invasion (p<0.0001), perineural invasion (p=0.005) and the tumor border configuration (p<0.0001). High GBd and PDCs-G grades correlate directly with other negative prognostic factors in CRC.Thus, these new parameters/classification methods could be used as additional tools for risk stratification in patients with CRC.","PeriodicalId":9147,"journal":{"name":"Bosnian journal of basic medical sciences","volume":"22 2","pages":"164-177"},"PeriodicalIF":3.4,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8977077/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39382781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Prognostic nomograms for predicting overall survival and cancer-specific survival of patients with very early-onset colorectal cancer: A population-based analysis 预测极早发性结直肠癌患者总生存期和癌症特异性生存期的预后图:一项基于人群的分析

IF 3.4 4区医学

Bosnian journal of basic medical sciences Pub Date : 2022-03-27 DOI: 10.17305/bjbms.2021.7035

B. Dong, Yuping Chen, G. Lyu

{"title":"Prognostic nomograms for predicting overall survival and cancer-specific survival of patients with very early-onset colorectal cancer: A population-based analysis","authors":"B. Dong, Yuping Chen, G. Lyu","doi":"10.17305/bjbms.2021.7035","DOIUrl":"https://doi.org/10.17305/bjbms.2021.7035","url":null,"abstract":"In contrast to the declining incidence in older populations, the incidence of very early-onset colorectal cancer (VEO-CRC) patients (aged ≤40 years) has been increasing in different regions of the world. In this study, we aimed to establish nomogram models for the prognostic prediction of patients with VEO-CRC for both overall survival (OS) and cancer-specific survival (CSS). Patients diagnosed with VEO-CRC between 2010 and 2015 from the Surveillance, Epidemiology, and End Results (SEER) database were collected and randomly assigned to the training cohort and validation cohort at a ratio of 7:3 for model construction and internal validation. Using univariate and multivariate Cox regression analysis to screen important variables, which were then used to construct a nomogram. The nomogram was evaluated using calibration curves and the receiver operating characteristic (ROC) curves. A total of 3061 patients were included and randomly divided into the training cohort (n = 2145) and validation cohort (n = 916). Five independent prognostic factors, including race, grade, tumor size, American Joint Commission on Cancer (AJCC) stage, and AJCC T stage, were all significantly identified in OS multivariate Cox regression analysis. Meanwhile, in CSS, multivariate Cox regression analysis demonstrated that race, grade, tumor size, AJCC stage, AJCC T stage, AJCC N stage, and SEER stage were independent prognostic factors. The calibration plots of the established nomograms indicated high correlations between the predicted and observed results. C-index and ROC analysis implied that our nomogram model has a strong predictive ability. Moreover, nomograms also showed higher C-index values compared to tumor-node-metastasis and SEER stages. We established and validated a simple-to-use nomogram to evaluate the 1-, 3-, and 5-year OS and CSS prognosis of patients with VEO-CRC. This tool can assist clinicians to optimize individualized treatment plans.","PeriodicalId":9147,"journal":{"name":"Bosnian journal of basic medical sciences","volume":"22 1","pages":"803 - 817"},"PeriodicalIF":3.4,"publicationDate":"2022-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41462637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Small cell variant of chromophobe renal cell carcinoma: Clinicopathologic and molecular-genetic analysis of 10 cases 嫌色肾细胞癌小细胞变异:10例临床病理及分子遗传学分析

IF 3.4 4区医学

Bosnian journal of basic medical sciences Pub Date : 2022-03-10 DOI: 10.17305/bjbms.2021.6935

J. Rogala, F. Kojima, R. Alaghehbandan, N. Ptáková, Ana Bravc, S. Bulimbasic, D. Perez Montiel, Maryna Slisarenko, L. Ali, Levente Kuthi, K. Pivovarcikova, Květoslava Michalová, Boris Bartovic, Adriena Bartos Vesela, O. Dolejsova, M. Michal, O. Hes

{"title":"Small cell variant of chromophobe renal cell carcinoma: Clinicopathologic and molecular-genetic analysis of 10 cases","authors":"J. Rogala, F. Kojima, R. Alaghehbandan, N. Ptáková, Ana Bravc, S. Bulimbasic, D. Perez Montiel, Maryna Slisarenko, L. Ali, Levente Kuthi, K. Pivovarcikova, Květoslava Michalová, Boris Bartovic, Adriena Bartos Vesela, O. Dolejsova, M. Michal, O. Hes","doi":"10.17305/bjbms.2021.6935","DOIUrl":"https://doi.org/10.17305/bjbms.2021.6935","url":null,"abstract":"The morphologic diversity of chromophobe renal cell carcinoma (ChRCC) is well-known. Aside from typical morphology, pigmented adenomatoid, multicystic, and papillary patterns have been described. Ten cases of CHRCC composed of small-cell population in various percentages were analyzed, using morphologic parameters, immunohistochemistry, and next-generation sequencing testing. Patients were five males and five females, with age ranging from 40 to 78 years. The size of tumors ranged from 2.2 cm to 11 cm (mean 5.17 cm). Small-cell component comprised 10 to 80% of the tumor volume, while the remaining was formed by cells with classic ChRCC morphology. The immunohistochemical profile of the small-cell component was consistent with typical ChRCC immunophenotype, with CD117 and CK7 positivity. Neuroendocrine markers were negative. Mutations of 13 genes were found: DCIER1, FGFR3, JAK3, SUFO, FAM46C, FANCG, MET, PLCG2, APC, POLE, EPICAM, MUTYH, and AR. However, only the PLCG2 mutation is considered pathogenic. The small-cell variant of ChRCC further highlights and expands on existing morphologic heterogeneity spectrum. Recognition of small-cell variant of CHRCC is not problematic in tumors, where the “classic” CHRCC component is present. However, in limited material (i.e., core biopsy), this may present a diagnostic challenge. Based on the limited follow-up data available, it appears that the small-cell tumor component had no impact on prognosis, since there was no aggressive behavior documented. Awareness of this unusual pattern and applying additional sections to find classic morphology of ChRCC, as well as excluding neuroendocrine nature by immunohistochemistry, may help resolve difficult cases.","PeriodicalId":9147,"journal":{"name":"Bosnian journal of basic medical sciences","volume":"22 1","pages":"531 - 539"},"PeriodicalIF":3.4,"publicationDate":"2022-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45854551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Identification and verification of the ferroptosis- and pyroptosis-associated prognostic signature for low-grade glioma 低级别胶质瘤铁下垂和热下垂相关预后特征的鉴定和验证

IF 3.4 4区医学

Bosnian journal of basic medical sciences Pub Date : 2022-03-09 DOI: 10.17305/bjbms.2021.6888

Jie Wang, Jie Ren, JiFeng Liu, Linyun Zhang, Q. Yuan, B. Dong

{"title":"Identification and verification of the ferroptosis- and pyroptosis-associated prognostic signature for low-grade glioma","authors":"Jie Wang, Jie Ren, JiFeng Liu, Linyun Zhang, Q. Yuan, B. Dong","doi":"10.17305/bjbms.2021.6888","DOIUrl":"https://doi.org/10.17305/bjbms.2021.6888","url":null,"abstract":"Accumulating evidence reveals that ferroptosis and pyroptosis play pivotal roles in tumorigenesis of low-grade glioma (LGG). In this research, we aimed to classify molecular subtypes and further identify and verify a novel multigene signature in LGG on the basis of ferroptosis- and pyroptosis-related genes (FPRGs). Raw sequencing data and corresponding clinical data of LGG samples retrieved from The Cancer Genome Atlas and Chinese Glioma Genome Atlas databases were obtained for the training and validation datasets. Non-negative matrix factorization (NMF) clustering defined by FPRGs associated with prognosis was performed to classify molecular subtypes of LGG patients. Least absolute shrinkage and selection operator-support vector machine-random forest analysis was carried out to develop a FPRG signature to predict the survival and benefit of immunotherapy of LGG patients. NMF clustering defined by FPRGs with prognostic values acted to categorize LGG patients into two molecular subtypes with different prognosis, clinical traits, and immune microenvironments. A six-FPRG prognostic signature was constructed, accompanied by the optimal p-value. The AUC values of our signature exhibited great prognostic performances. Our signature was superior to other four well-recognized signatures in predicting the survival probability of LGG patients. Immune characteristics, tumor mutation profile, tumor stemness indices, MGMT methylation, and immunotherapy response biomarkers showed significant differences between high- and low-risk populations. Finally, a nomogram was created for quantitative prediction of the survival probability of LGG patients, with the AUC values of the nomogram being 0.916, 0.888, and 0.836 for 1-, 3-, and 5-year survival, sequentially. Overall, the FPRG signature may function as an effective indicator for the prognosis prediction and immunotherapy response of LGG patients.","PeriodicalId":9147,"journal":{"name":"Bosnian journal of basic medical sciences","volume":"22 1","pages":"728 - 750"},"PeriodicalIF":3.4,"publicationDate":"2022-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41675403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Thiersch graft follow-up with narrow band imaging for acquired atresia of the external auditory canal: Canaloplasty with Thiersch graft versus vascularization evaluated with narrow band imaging Thiersch移植物窄带成像治疗获得性外耳道闭锁的随访：Thiersch移植管成形术与窄带成像评价血管化

IF 3.4 4区医学

Bosnian journal of basic medical sciences Pub Date : 2022-03-02 DOI: 10.17305/bjbms.2021.6876

A. Pace, V. Rossetti, I. C. Visconti, A. Milani, G. Iannella, A. Maniaci, S. Cocuzza, G. Magliulo

{"title":"Thiersch graft follow-up with narrow band imaging for acquired atresia of the external auditory canal: Canaloplasty with Thiersch graft versus vascularization evaluated with narrow band imaging","authors":"A. Pace, V. Rossetti, I. C. Visconti, A. Milani, G. Iannella, A. Maniaci, S. Cocuzza, G. Magliulo","doi":"10.17305/bjbms.2021.6876","DOIUrl":"https://doi.org/10.17305/bjbms.2021.6876","url":null,"abstract":"Acquired atresia of the external ear canal (EAC) is defined as a narrowing caused by abnormal production of soft or bony tissue. EAC has two different phases, a wet one and a dry one. A computed tomography (CT) scan may show images where the soft tissue fills the EAC. Treatment with CT may be medical and/or surgical. The surgical technique most widely adopted is canaloplasty with a skin free flap. To the best of our knowledge, no previous article has reported data analyzing the vascularization of acquired atresia of the EAC and vascularization of the skin flap during follow-up with narrow-band imaging (NBI). This paper aimed to study post-surgical vascularization of the skin graft, with NBI endoscopy, to identify any eventual areas with less perfusion that may lead to degeneration and fibrosis. Patients suffering from acquired atresia of the external auditory canal, surgically treated in the Department of Organi di Senso of Sapienza University, from 2017 to 2020 were enrolled. All patients underwent anamnestic collection, physical examination, and mastoid CT. Pre- and post-operative otoendoscopic evaluations (at 1, 3, 6 and 12 months) were performed with both cold white light (CWL) and NBI endoscopic vision. 17 patients were enrolled in the study. Pre-operative otoendoscopic examination of CWL showed stenosis with a diameter <75% and a tympanic membrane not viewable in all patients. At 12 months of follow-up, 94% of patients had no recurrence of EAC stenosis. 88% of patients presented an adequate vascularization by NBI. Our study aimed to evaluate whether the NBI endoscopic view and the analysis of vascularization may be useful for improving the prognosis of patients surgically treated with canaloplasty and Thiersch graft for acquired atresia of EAC, concerning the single analysis using CWL endoscope.","PeriodicalId":9147,"journal":{"name":"Bosnian journal of basic medical sciences","volume":"22 1","pages":"798 - 802"},"PeriodicalIF":3.4,"publicationDate":"2022-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49649414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Association between fasting blood glucose and thyroid stimulating hormones and suicidal tendency and disease severity in patients with major depressive disorder 重度抑郁症患者空腹血糖和促甲状腺激素与自杀倾向和疾病严重程度的关系

IF 3.4 4区医学

Bosnian journal of basic medical sciences Pub Date : 2022-02-28 DOI: 10.17305/bjbms.2021.6754

Weiting Liu, Zezhen Wu, Min Sun, Shuo Zhang, Juan Yuan, D. Zhu, Guimimg Yan, Kaijian Hou

{"title":"Association between fasting blood glucose and thyroid stimulating hormones and suicidal tendency and disease severity in patients with major depressive disorder","authors":"Weiting Liu, Zezhen Wu, Min Sun, Shuo Zhang, Juan Yuan, D. Zhu, Guimimg Yan, Kaijian Hou","doi":"10.17305/bjbms.2021.6754","DOIUrl":"https://doi.org/10.17305/bjbms.2021.6754","url":null,"abstract":"Thyroid dysfunction and diabetes are reported to be associated with depression. However, their role in the suicide risk in patients with major depressive disorder (MDD) is unclear. The purpose of this study was to investigate and compare thyroid dysfunction and diabetes between suicide attempters and non-suicide attempters in a large sample of first-episode drug-naïve MDD patients. A descriptive study was conducted on 1279 Chinese outpatients with a diagnosis of first-episode MDD. Their socio-demographic information, blood levels of thyroid hormones, glucose, lipids and body mass index (BMI) parameters were collected. The positive subscales of the positive and negative syndrome scale (PANSS), Hamilton anxiety rating scale (HAMA), Hamilton depression rating scale (HAMD) were measured for psychotic, anxiety and depressive symptoms. Our results showed that compared with non-suicide attempters (p < 0.01), suicide attempters had statistically higher scores on HAMD, HAMA and PANSS psychotic symptoms, as well as higher thyroid stimulating hormone (TSH) serum levels, glucose, anti-thyroglobulin (A-TG), anti-thyroid peroxidase (A-TPO), total cholesterol (TC), triglycerides (TG), low density lipoprotein cholesterol (LDL-C), systolic blood pressure, and diastolic blood pressure (all with p < 0.001). These results revealed that TSH, A-TG, A-TPO, TC, TG, and LDL-C may be promising biomarkers of suicide risk in MDD, implying the importance of regular assessment of blood glucose level and thyroid function parameters for suicide prevention, along with possible treatment for impaired thyroid function and diabetes for the suicide intervention in MDD patients. Such patients with abnormal blood sugar and TSH must undergo thorough screening for suicidal ideation.","PeriodicalId":9147,"journal":{"name":"Bosnian journal of basic medical sciences","volume":"22 1","pages":"635 - 642"},"PeriodicalIF":3.4,"publicationDate":"2022-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48863249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 24

Circular RNA circ_0020014 contributes to osteoarthritis progression via miR-613/ADAMTS5 axis 环状RNA circ_0020014通过miR-613/ADAMTS5轴促进骨关节炎进展

IF 3.4 4区医学

Bosnian journal of basic medical sciences Pub Date : 2022-02-28 DOI: 10.17305/bjbms.2021.6668

Zirui Yu, F. Cong, Wentao Zhang, Tao Song, Shihui Zhang, Renqi Jiang

{"title":"Circular RNA circ_0020014 contributes to osteoarthritis progression via miR-613/ADAMTS5 axis","authors":"Zirui Yu, F. Cong, Wentao Zhang, Tao Song, Shihui Zhang, Renqi Jiang","doi":"10.17305/bjbms.2021.6668","DOIUrl":"https://doi.org/10.17305/bjbms.2021.6668","url":null,"abstract":"Circular RNAs have been shown to be significant regulators in osteoarthritis (OA), whereas the functional effect of circ_0020014 in OA remains unclear. Our goal was to try and understand the underlying regulatory mechanism of circ_0020014 in OA. The cartilage tissue was obtained from OA patients and trauma patients. Interleukin-1β (IL-1β)-treated chondrocytes (CHON-001) were used as the in vitro cellular model for OA. The expression levels of circ_0020014, microRNA-613 (miR-613), and a disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS5) were examined by real-time quantitative polymerase chain reaction. The protein level was detected using the Western blot assay. Cell viability and apoptosis were measured by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-2H-tetrazol-3-ium bromide and flow cytometry assays, respectively. The secretion of inflammatory cytokine was determined by enzyme-linked immunosorbent assay. Circ_0020014 was upregulated in OA cartilage tissues and IL-1β-treated CHON-001 cells, compared with that in healthy cartilage tissues and untreated cells. IL-1β treatment induced cell injury by promoting inflammation and apoptosis, and inhibiting cell viability and extracellular matrix accumulation in chondrocytes. Circ_0020014 knockdown significantly protected CHON-001 cells from IL-1β-induced cell dysfunction. MiR-613 was targeted by circ_0020014 and negatively regulated ADAMTS5 expression. In addition, miR-613 downregulation or ADAMTS5 overexpression partly lessened the protective effect of circ_0020014 knockdown on IL-1β-treated CHON-001 cells. Collectively, circ_0020014 acted as a miR-613 sponge to regulate ADAMTS5 expression, thereby protecting chondrocytes from IL-1β-induced inflammatory damage, which might be a novel diagnostic marker for OA.","PeriodicalId":9147,"journal":{"name":"Bosnian journal of basic medical sciences","volume":"22 1","pages":"716 - 727"},"PeriodicalIF":3.4,"publicationDate":"2022-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45124964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5