Bosnian journal of basic medical sciences最新文献_第5页

Three-dimensional telomere profiles in papillary thyroid cancer variants: A pilot study. 乳头状甲状腺癌变异的三维端粒谱:一项初步研究。

IF 3.4 4区医学

Bosnian journal of basic medical sciences Pub Date : 2022-06-01 DOI: 10.17305/bjbms.2021.6639

Aline Rangel-Pozzo, Tinuccia Dettori, Daniela Virginia Frau, Federica Etzi, John Gartner, Garbor Fisher, Roberta Vanni, Sabine Mai, Paola Caria

{"title":"Three-dimensional telomere profiles in papillary thyroid cancer variants: A pilot study.","authors":"Aline Rangel-Pozzo, Tinuccia Dettori, Daniela Virginia Frau, Federica Etzi, John Gartner, Garbor Fisher, Roberta Vanni, Sabine Mai, Paola Caria","doi":"10.17305/bjbms.2021.6639","DOIUrl":"https://doi.org/10.17305/bjbms.2021.6639","url":null,"abstract":"Besides the two main histologic types of papillary thyroid carcinoma (PTC), the classical PTC (CL-PTC) and the follicular variant PTC (FV-PTC), several other variants are described. The encapsulated FV-PTC variant was recently reclassified as noninvasive follicular thyroid neoplasm with papillary-like nuclear features (NIFTP) due to its similarities to benign lesions. Specific molecular signatures, however, are still unavailable. It is well known that improper DNA repair of dysfunctional telomeres may cause telomere-related genome instability. The mechanisms involved in the damaged telomere repair processing may lead to detrimental outcomes, altering the three-dimensional (3D) nuclear telomere and genome organization in cancer cells. This pilot study aimed to evaluate whether a specific 3D nuclear telomere architecture might characterize NIFTP, potentially distinguishing it from other PTC histologic variants. Our findings demonstrate that 3D telomere profiles of CL-PTC and FV-PTC were different from NIFTP and that NIFTP more closely resembles follicular thyroid adenoma (FTA). NIFTP has longer telomeres than CL-PTC and FV-PTC samples, and the telomere length of NIFTP overlaps with that of the FTA histotype. In contrast, there was no association between BRAF expression and telomere length in all tested samples. These preliminary findings reinforce the view that NIFTP is closer to non-malignant thyroid nodules and confirm that PTC features short telomeres.","PeriodicalId":9147,"journal":{"name":"Bosnian journal of basic medical sciences","volume":" ","pages":"481-487"},"PeriodicalIF":3.4,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9162746/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39705809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Risk and prognosis of secondary bladder cancer after post-operative radiotherapy for gynecological cancer. 妇科肿瘤放疗后继发膀胱癌的风险及预后。

IF 3.4 4区医学

Bosnian journal of basic medical sciences Pub Date : 2022-06-01 DOI: 10.17305/bjbms.2021.6338

Li Wen, Guansheng Zhong, Yingjiao Zhang, Miaochun Zhong

{"title":"Risk and prognosis of secondary bladder cancer after post-operative radiotherapy for gynecological cancer.","authors":"Li Wen, Guansheng Zhong, Yingjiao Zhang, Miaochun Zhong","doi":"10.17305/bjbms.2021.6338","DOIUrl":"https://doi.org/10.17305/bjbms.2021.6338","url":null,"abstract":"The aim of this study was to investigate the impacts of radiation therapy (RT) on the occurrence risk of secondary bladder cancer (SBC) and on the patients' survival outcome after being diagnosed with gynecological cancer (EC). The data was obtained from the SEER database between 1973 and 2015. Chi-squared test was used to compare the clinicopathological characteristics among the different groups. Fine and Gray's competing risk model was used to assess the cumulative incidence and occurrence risk of SBC in GC survivors. Kaplan-Meier method was utilized for survival analysis. A total of 123,476 GC patients were included, among which 31,847 (25.8%) patients received RT while 91629 (74.2%) patients did not. The cumulative incidence of SBC was 1.59% or 0.73% among patients who had received prior GC specific RT or not, respectively. All EBRT (standardized incidence ratio (SIR) =2.49, 95% CI [2.17-2.86]), brachytherapy (SIR =1.96, 95% CI [1.60-2.38]), and combinational RT modality groups (SIR =2.73, 95% CI [2.24-3.28]) had dramatically higher SBC incidence as compared to the US general population. Receiving EBRT (HR = 2.83, 95% CI [2.34-3.43]), brachytherapy (HR = 2.17, 95% CI [1.67-2.82]), and combinational RT modality (HR = 2.97, 95% CI [2.34-3.77]) were independent risk factors for SBC development. Survival detriment was observed in SBC patients who received RT after GC diagnosis, as compared to those who did not receive RT. In conclusion, patients who underwent RT after GC had an increased risk of developing bladder as a secondary primary cancer. A long-term surveillance for SBC occurrence is necessary for GC patients who have received prior RT.","PeriodicalId":9147,"journal":{"name":"Bosnian journal of basic medical sciences","volume":"22 3","pages":"471-480"},"PeriodicalIF":3.4,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9162740/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10300722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Development and validation of a novel pre-operative comprehensive prognostic score in esophageal squamous cell carcinoma. 一种新的食管鳞状细胞癌术前综合预后评分方法的建立和验证。

IF 3.4 4区医学

Bosnian journal of basic medical sciences Pub Date : 2022-06-01 DOI: 10.17305/bjbms.2021.6350

Jifeng Feng, Liang Wang, Xun Yang, Qixun Chen

{"title":"Development and validation of a novel pre-operative comprehensive prognostic score in esophageal squamous cell carcinoma.","authors":"Jifeng Feng, Liang Wang, Xun Yang, Qixun Chen","doi":"10.17305/bjbms.2021.6350","DOIUrl":"https://doi.org/10.17305/bjbms.2021.6350","url":null,"abstract":"We herein propose a novel integrative score based on inflammatory and nutritional score, coagulation indicator and tumor marker, named comprehensive prognostic score (CPS), to predict postoperative survival in resectable esophageal squamous cell carcinoma (ESCC). We also aimed to establish and validate a nomogram based on CPS and other clinical features for individual survival prediction. A total of 490 resectable ESCC patients were randomly divided into either a training or validation cohort at a ratio of 7:3 for retrospective analysis. The CPS, based on squamous cell carcinoma antigen (SCCA), C-reactive protein to albumin ratio (CAR), neutrophil to lymphocyte ratio (NLR), and fibrinogen (FIB), was divided into two models to verify its prognostic value. The predictive model of CPS-based nomogram was established and validated in two cohorts. Patients with CPS low group in model 1 had better 5-year cancer-specific survival (CSS) than those in CPS high group (50.7% vs. 17.8%, P<0.001). For model 2, the 5-year CSS for CPS 0, 1 and 2 were 75.0%, 38.9% and 13.3%, respectively (P<0.001). CPS was confirmed as an independent prognostic score in both models. The CPS-based nomogram can accurately and effectively predict survival in resected ESCC. The CPS is a novel, simple, and effective predictor in resectable ESCC. Moreover, CPS has a potential independent prognostic value in predicting postoperative survival, which can accurately and effectively predict individual survival in resectable ESCC.","PeriodicalId":9147,"journal":{"name":"Bosnian journal of basic medical sciences","volume":" ","pages":"460-470"},"PeriodicalIF":3.4,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9162752/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39541207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Six RNA binding proteins (RBPs) related prognostic model predicts overall survival for clear cell renal cell carcinoma and is associated with immune infiltration. 六种RNA结合蛋白(rbp)相关的预后模型预测透明细胞肾细胞癌的总体生存并与免疫浸润相关。

IF 3.4 4区医学

Bosnian journal of basic medical sciences Pub Date : 2022-06-01 DOI: 10.17305/bjbms.2021.6097

Qianwei Xing, Jiaochen Luan, Shouyong Liu, Limin Ma, Yi Wang

{"title":"Six RNA binding proteins (RBPs) related prognostic model predicts overall survival for clear cell renal cell carcinoma and is associated with immune infiltration.","authors":"Qianwei Xing, Jiaochen Luan, Shouyong Liu, Limin Ma, Yi Wang","doi":"10.17305/bjbms.2021.6097","DOIUrl":"https://doi.org/10.17305/bjbms.2021.6097","url":null,"abstract":"The aim of this article was to construct an accurate prognostic model by using RNA-binding proteins (RBPs) to predict overall survival (OS) for patients with clear cell renal cell carcinoma (ccRCC) as well as to reveal its associations with immune infiltration. Expression profiles based on RNA-binding proteins (RBPs) and clinical follow-up parameters were obtained from the Cancer Genome Atlas (TCGA) and the ArrayExpress databases. Through univariate COX and LASSO regression analyses, the RBPs based signature was developed. A total of six RBPs (CLK2, IGF2BP2, RNASE2, EZH2, PABPC1L, RPL22L1) were eventually used to establish a prognostic signature. Based on this signature, ccRCC patients were classified into high-risk and low-risk subgroups and significant OS was obtained in both the internal and external datasets (p<0.05). AUCs of its ROC curve were all above 0.70 and this signature was an independent prognostic factor of OS for ccRCC (p<0.05). Nomograms were also constructed to visualize the relationships among individual predictors and 1-, 3- and 5-year OS for ccRCC. Furthermore, the established RBPs based signature was strongly related to critical clinicopathologic characteristics such as grade (p=8.921e-12), stage (p=1.421e-11), M (p=1.662e-05), and T stage (p=7.907e-10). Moreover, 12 kinds of tumor-infiltrating immune cells were significantly linked to high-risk and low-risk groups classified by our constructed model (all p<0.05). Our study successfully identified six RBPs as a robust prognostic signature in ccRCC by both external and internal verification. Besides, our established model displayed significant associations with immune infiltration. In addition to original clinical parameters, our findings may further help clinicians in predicting patients' survival status and creating individualized treatment plans.","PeriodicalId":9147,"journal":{"name":"Bosnian journal of basic medical sciences","volume":" ","pages":"435-452"},"PeriodicalIF":3.4,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9162755/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39346965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

LncRNA ADAMTS9-AS1 knockdown suppresses cell proliferation and migration in glioma through downregulating Wnt/β-catenin signaling pathway. LncRNA ADAMTS9-AS1敲低通过下调Wnt/β-catenin信号通路抑制胶质瘤细胞增殖和迁移。

IF 3.4 4区医学

Bosnian journal of basic medical sciences Pub Date : 2022-06-01 DOI: 10.17305/bjbms.2021.6199

Chunhui Zhou, Hulin Zhao, Shuiwei Wang, Chao Dong, Fan Yang, Jianning Zhang

{"title":"LncRNA ADAMTS9-AS1 knockdown suppresses cell proliferation and migration in glioma through downregulating Wnt/β-catenin signaling pathway.","authors":"Chunhui Zhou, Hulin Zhao, Shuiwei Wang, Chao Dong, Fan Yang, Jianning Zhang","doi":"10.17305/bjbms.2021.6199","DOIUrl":"https://doi.org/10.17305/bjbms.2021.6199","url":null,"abstract":"The long non-coding RNA antisense 1 ADAMTS9-AS1 has been reported to serve as an oncogene or tumor suppressor in several tumors, including colorectal cancer and hepatocellular carcinoma. Nevertheless, the clinical significance and biological behaviors of ADAMTS9-AS1 in glioma still remain unclear. Therefore, the goal of this study was to evaluate the functional roles and potential mechanisms of ADAMTS9-AS1 in glioma cells. Using quantitative real-time PCR analysis, we found that ADAMTS9-AS1 was upregulated in glioma tissues and cells in comparison to corresponding controls. ADAMTS9-AS1 expression level was correlated to tumor size (p=0.005) and WHO grade (p=0.002). Kaplan-Meier analysis and Cox multivariate analysis showed that ADAMTS9-AS1 could serve as an independent prognostic factor affecting the overall survival of glioma patients. Functionally, depletion of ADAMTS9-AS1 significantly suppressed the proliferation, migration and invasion in glioma cell lines (U251 and U87), as shown via CCK-8 assay, Edu corporation assay, wound healing assay and transwell assay. Furthermore, we demonstrated that knockdown of ADAMTS9-AS1 suppressed Wnt1, β-catenin, c-myc and PCNA, while upregulating E-cadherin expression. In conclusion, our data revealed that ADAMTS9-AS1 confers oncogenic function in the progression of glioma, thus targeting ADAMTS9-AS1 might be a promising therapeutic strategy for this disease.","PeriodicalId":9147,"journal":{"name":"Bosnian journal of basic medical sciences","volume":" ","pages":"395-402"},"PeriodicalIF":3.4,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9162748/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39738379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Whole-exome sequencing reveals rare genetic variations in ovarian granulosa cell tumor. 全外显子组测序揭示卵巢颗粒细胞瘤罕见的遗传变异。

IF 3.4 4区医学

Bosnian journal of basic medical sciences Pub Date : 2022-06-01 DOI: 10.17305/bjbms.2021.6789

Seungyeon Kim, Songmi Kim, Seyoung Mun, Yongsik Kwak, Kwang-Sun Suh, Song-Yi Choi, Kyudong Han

{"title":"Whole-exome sequencing reveals rare genetic variations in ovarian granulosa cell tumor.","authors":"Seungyeon Kim, Songmi Kim, Seyoung Mun, Yongsik Kwak, Kwang-Sun Suh, Song-Yi Choi, Kyudong Han","doi":"10.17305/bjbms.2021.6789","DOIUrl":"https://doi.org/10.17305/bjbms.2021.6789","url":null,"abstract":"Ovarian granulosa cell tumor (OGCT) is a rare ovarian tumor that accounts for about 2-5% of all ovarian tumors. Despite the low grade of ovarian tumors, high and late recurrences are common in OGCT patients. Even though this tumor usually occurs in adult women with high estrogen levels, the cause of OGCT is still unknown. To screen genetic variants associated with OGCT, we collected normal and matched-tumor formalin-fixed paraffin-embedded from 11 OGCT patients and performed whole-exome sequencing using Illumina NovaSeq 6000. A total of 1,067,219 single nucleotide polymorphisms (SNPs) and 162,155 insertions/deletions (indels) were identified from 11 pairs of samples. Of these, we identified 44 tumor-specific SNPs in 22 genes and four tumor-specific indels in one gene that were common to 11 patients. We used three cancer databases (TCGA, COSMIC, and ICGC) to investigate genes associated with ovarian cancers. Nine genes (SEC22B, FEZ2, ANKRD36B, GYPA, MUC3A, PRSS3, NUTM2A, OR8U1, and KRTAP10-6) associated with ovarian cancers were found in all three databases. In addition, we identified seven rare variants with MAF ≤ 0.05 in two genes (PRSS3 and MUC3A). Of seven rare variants, five variants in MUC3A are potentially pathogenic. Furthermore, we conducted gene enrichment analysis of tumor-specific 417 genes in SNPs and 106 genes in indels using cytoscape and metascape. In GO analysis, these genes were highly enriched in “selective autophagy,” and “regulation of anoikis.” Taken together, we suggest that MUC3A is implicated in OGCT development, and MUC3A could be used as a potential biomarker for OGCT diagnosis.","PeriodicalId":9147,"journal":{"name":"Bosnian journal of basic medical sciences","volume":" ","pages":"403-411"},"PeriodicalIF":3.4,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9162744/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39917394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Impact of smoking on the incidence and post-operative complications of total knee arthroplasty: A systematic review and meta-analysis of cohort studies. 吸烟对全膝关节置换术发生率和术后并发症的影响：队列研究的系统回顾和荟萃分析。

IF 3.1 4区医学

Bosnian journal of basic medical sciences Pub Date : 2022-06-01 DOI: 10.17305/bjbms.2021.6538

Yuqi He, Mohamed Omar, Xiaoyuan Feng, Claudia Neunaber, Michael Jagodzinski

{"title":"Impact of smoking on the incidence and post-operative complications of total knee arthroplasty: A systematic review and meta-analysis of cohort studies.","authors":"Yuqi He, Mohamed Omar, Xiaoyuan Feng, Claudia Neunaber, Michael Jagodzinski","doi":"10.17305/bjbms.2021.6538","DOIUrl":"10.17305/bjbms.2021.6538","url":null,"abstract":"Osteoarthritis and rheumatoid arthritis are the most ubiquitous joint disorders which cause tremendous loss of life quality and impose an economic burden on society. At present, the treatment options for these two diseases comprise non-operative and surgical treatments, amongst those total knee arthroplasties (TKA). Various studies have recognized smoking as a significant risk factor for postoperative complications. Therefore, the purpose of this study was to examine the impact of smoking on the incidence and postoperative complications after a total knee arthroplasty by a systematic review and meta-analysis. The research was performed using PUBMED, Cochrane Library and EMBASE, extracting data from thirteen suitable studies and incorporating 2,109,482 patients. Cohort studies evaluating the impact of smoking on TKA with sufficient data were included for the study, and cohort studies without a proper control group and complete data were excluded. A fixed-effects or random-effects model was used to measure the pooled risk ratio (RR) or hazard ratio (HR) with 95% confidence interval (CI). Compared to non-smokers, smokers had a significantly lower incidence of TKA (p<0.01). However, smokers had a higher incidence of total complications (p=0.01), surgical complications (p<0.01), pneumonia (p<0.01) and revision surgery (p=0.01). No significant difference in the risk of blood transfusion (p=0.42), deep vein thrombosis (p=0.31), pulmonary embolism (p=0.34), urinary tract infection (p=0.46) or mortality (p=0.39) was found between smokers and non-smokers. In conclusion, the study indicated that tobacco has two diametrically opposite effects on TKA patients: 1. Tobacco increases the incidence of surgical complications, pneumonia and revision after TKA; 2. It decreases the overall risk of being a candidate for TKA.","PeriodicalId":9147,"journal":{"name":"Bosnian journal of basic medical sciences","volume":" ","pages":"353-365"},"PeriodicalIF":3.1,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9162741/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39738378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of the vitality tests used in the dental clinical practice and histological analysis of the dental pulp. 牙科临床应用活力试验与牙髓组织学分析的比较。

IF 3.4 4区医学

Bosnian journal of basic medical sciences Pub Date : 2022-06-01 DOI: 10.17305/bjbms.2021.6841

Ana Tenyi, Lidija Nemeth, Aljaž Golež, Ksenija Cankar, Aleksandra Milutinović

{"title":"Comparison of the vitality tests used in the dental clinical practice and histological analysis of the dental pulp.","authors":"Ana Tenyi, Lidija Nemeth, Aljaž Golež, Ksenija Cankar, Aleksandra Milutinović","doi":"10.17305/bjbms.2021.6841","DOIUrl":"https://doi.org/10.17305/bjbms.2021.6841","url":null,"abstract":"In dentistry, indirect diagnostic methods such as electrical sensibility testing and pulse oximetry are used to assess the status of the pulp. Our study aimed to determine the correlation between hemoglobin oxygen saturation and vascular volume density (Vvasc). We also wanted to examine an electrical sensibility test and the volume density of myelinated nerve fibers (Vnerv). Twenty-six intact permanent premolars were included in the study. For histological analysis, the pulp tissue was stained with hematoxylin-eosin and immunohistochemically for von Willebrand factor and S100 to detect blood vessels and myelinated nerve fibers, respectively. The stereological analysis was used to determine the Vvasc and Vnerv. Statistical analysis was done using the Pearson correlation test and Welch's ANOVA test. Histological analysis showed that the pulp tissue was strongly vascularized and innervated. A significant positive correlation was found between Vvasc and hemoglobin oxygen saturation levels (p=0.030). A significant negative correlation was found between Vnerv and the lowest electrical voltage that patient felt (p=0.033). According to the maturity of the dental apex, teeth were divided into a group with open (N=6, OA group) and closed apex (N=20, CA group). We found that pulps in the CA group had higher Vnerv than the OA group (p=0.037). In contrast, there were no significant differences in Vvasc of the pulp tissue (p=0.059), oxygen saturation (p=0.907), or electrical voltage (p=0.113) between both groups. We can conclude that the measurement of pulse oximetry and electrical sensibility test reflect the morphology of healthy pulp tissue independently of the maturity of the dental apex.","PeriodicalId":9147,"journal":{"name":"Bosnian journal of basic medical sciences","volume":" ","pages":"374-381"},"PeriodicalIF":3.4,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9162753/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39787891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

The first detection of Pneumocystis jirovecii in asthmatic patients post-COVID-19 in Jordan 约旦首次在covid -19后哮喘患者中检测到耶氏肺囊虫

IF 3.4 4区医学

Bosnian journal of basic medical sciences Pub Date : 2022-05-17 DOI: 10.17305/bjbms.2022.7335

A. Alsayed, Wamidh H. Talib, Abdullah Al-Dulaimi, Safa Daoud, Mohammed Al Maqbali

{"title":"The first detection of Pneumocystis jirovecii in asthmatic patients post-COVID-19 in Jordan","authors":"A. Alsayed, Wamidh H. Talib, Abdullah Al-Dulaimi, Safa Daoud, Mohammed Al Maqbali","doi":"10.17305/bjbms.2022.7335","DOIUrl":"https://doi.org/10.17305/bjbms.2022.7335","url":null,"abstract":"Pneumocystis jirovecii pneumonia (PCP), caused by fungal species named P. jirovecii, is a frequent opportunistic infection in those with human immunodeficiency virus infection. However, PCP has been documented in immunocompetent patients. This study aims to determine if P. jirovecii detection occurs in asthma patients following coronavirus disease 2019 (COVID-19) in a Jordanian cohort. Another aim was to evaluate a method of TaqMan quantitative polymerase chain reaction (qPCR) assay to detect P. jirovecii, from sputum samples. The nasopharyngeal swabs were used to detect SARS-CoV-2 and sputum samples were tested for P. jirovecii using real-time qPCR assay. Beta-tubulin (BT) and dihydrofolate reductase (DHFR) genes were the directed targets of P. jirovecii. The results showed that the mean qPCR efficiencies of BT and DHFR were 96.37% and 100.13%, respectively. Three out of 31 included patients (9.7%) had a positive P. jirovecii. All of the three patients had used oral corticosteroids (OCS) in the past 2 months due asthma exacerbation and were treated with OCS for COVID-19. This is the first study based in Jordan to demonstrate that P. jirovecii and COVID-19 can coexist and that it is important to maintain a broad differential diagnosis, especially in immunocompromised patients. Chronic lung disease can be a risk factor for the P. jirovecii colonization possibly due to corticosteroid’s immunosuppression.","PeriodicalId":9147,"journal":{"name":"Bosnian journal of basic medical sciences","volume":"22 1","pages":"784 - 790"},"PeriodicalIF":3.4,"publicationDate":"2022-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46912186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

An improved joint non-negative matrix factorization for identifying surgical treatment timing of neonatal necrotizing enterocolitis 一种改进的关节非负矩阵因子分解法用于确定新生儿坏死性小肠结肠炎的手术治疗时机

IF 3.4 4区医学

Bosnian journal of basic medical sciences Pub Date : 2022-05-15 DOI: 10.17305/bjbms.2022.7046

Guoqiang Qi, Shou-jiang Huang, Dengming Lai, Jing Li, Yonggen Zhao, C.C.K. Shen, Jian Huang, Tianmei Liu, Kai Wei, Jinfa Dou, Q. Shu, Gang Yu

{"title":"An improved joint non-negative matrix factorization for identifying surgical treatment timing of neonatal necrotizing enterocolitis","authors":"Guoqiang Qi, Shou-jiang Huang, Dengming Lai, Jing Li, Yonggen Zhao, C.C.K. Shen, Jian Huang, Tianmei Liu, Kai Wei, Jinfa Dou, Q. Shu, Gang Yu","doi":"10.17305/bjbms.2022.7046","DOIUrl":"https://doi.org/10.17305/bjbms.2022.7046","url":null,"abstract":"Neonatal necrotizing enterocolitis is a severe neonatal intestinal disease. Timely, the identification of surgical indications is essential for newborns to seek the best time for treatment and improve prognosis. This paper attempts to establish an algorithm model based on multimodal clinical data to determine the features of surgical indications and construct an auxiliary diagnosis model. The proposed algorithm adds hypergraph constraints on the two modal data based on Joint Non-negative Matrix Factorization, aiming to mine the higher-order correlations of the two data features. In addition, the adjacency matrix of the two kinds of data is used as a network regularization constraint to prevent overfitting. Orthogonal and L1-norm regulations were introduced to avoid feature redundancy and perform feature selection, respectively, and confirmed 14 clinical features. Finally, we used three classifiers, random forest, support vector machine, and logistic regression, to perform binary classification of patients requiring surgery. The results show that when the features selected by the proposed algorithm model are classified by random forest, the area under the ROC curve is 0.8, which has high prediction accuracy.","PeriodicalId":9147,"journal":{"name":"Bosnian journal of basic medical sciences","volume":"22 1","pages":"972 - 981"},"PeriodicalIF":3.4,"publicationDate":"2022-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48975827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1