LncRNA ADAMTS9-AS1 knockdown suppresses cell proliferation and migration in glioma through downregulating Wnt/β-catenin signaling pathway.

IF 3.4 4区 医学 Q2 MEDICINE, RESEARCH & EXPERIMENTAL
Chunhui Zhou, Hulin Zhao, Shuiwei Wang, Chao Dong, Fan Yang, Jianning Zhang
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引用次数: 3

Abstract

The long non-coding RNA antisense 1 ADAMTS9-AS1 has been reported to serve as an oncogene or tumor suppressor in several tumors, including colorectal cancer and hepatocellular carcinoma. Nevertheless, the clinical significance and biological behaviors of ADAMTS9-AS1 in glioma still remain unclear. Therefore, the goal of this study was to evaluate the functional roles and potential mechanisms of ADAMTS9-AS1 in glioma cells. Using quantitative real-time PCR analysis, we found that ADAMTS9-AS1 was upregulated in glioma tissues and cells in comparison to corresponding controls. ADAMTS9-AS1 expression level was correlated to tumor size (p=0.005) and WHO grade (p=0.002). Kaplan-Meier analysis and Cox multivariate analysis showed that ADAMTS9-AS1 could serve as an independent prognostic factor affecting the overall survival of glioma patients. Functionally, depletion of ADAMTS9-AS1 significantly suppressed the proliferation, migration and invasion in glioma cell lines (U251 and U87), as shown via CCK-8 assay, Edu corporation assay, wound healing assay and transwell assay. Furthermore, we demonstrated that knockdown of ADAMTS9-AS1 suppressed Wnt1, β-catenin, c-myc and PCNA, while upregulating E-cadherin expression. In conclusion, our data revealed that ADAMTS9-AS1 confers oncogenic function in the progression of glioma, thus targeting ADAMTS9-AS1 might be a promising therapeutic strategy for this disease.

Abstract Image

Abstract Image

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LncRNA ADAMTS9-AS1敲低通过下调Wnt/β-catenin信号通路抑制胶质瘤细胞增殖和迁移。
据报道,长链非编码RNA反义1 ADAMTS9-AS1在包括结直肠癌和肝细胞癌在内的几种肿瘤中作为癌基因或肿瘤抑制因子。然而,ADAMTS9-AS1在胶质瘤中的临床意义和生物学行为尚不清楚。因此,本研究的目的是评估ADAMTS9-AS1在胶质瘤细胞中的功能作用及其潜在机制。通过实时荧光定量PCR分析,我们发现与相应的对照相比,ADAMTS9-AS1在胶质瘤组织和细胞中表达上调。ADAMTS9-AS1表达水平与肿瘤大小(p=0.005)和WHO分级(p=0.002)相关。Kaplan-Meier分析和Cox多因素分析显示,ADAMTS9-AS1可作为影响胶质瘤患者总生存的独立预后因素。功能上,CCK-8实验、Edu公司实验、伤口愈合实验和transwell实验显示,ADAMTS9-AS1的缺失显著抑制胶质瘤细胞系(U251和U87)的增殖、迁移和侵袭。此外,我们证实,ADAMTS9-AS1的敲低抑制Wnt1、β-catenin、c-myc和PCNA,同时上调E-cadherin的表达。总之,我们的数据显示ADAMTS9-AS1在胶质瘤的进展中具有致癌功能,因此靶向ADAMTS9-AS1可能是一种有希望的治疗胶质瘤的策略。
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来源期刊
Bosnian journal of basic medical sciences
Bosnian journal of basic medical sciences 医学-医学:研究与实验
CiteScore
7.40
自引率
5.90%
发文量
98
审稿时长
35 days
期刊介绍: The Bosnian Journal of Basic Medical Sciences (BJBMS) is an international, English-language, peer reviewed journal, publishing original articles from different disciplines of basic medical sciences. BJBMS welcomes original research and comprehensive reviews as well as short research communications in the field of biochemistry, genetics, immunology, microbiology, pathology, pharmacology, pharmaceutical sciences and physiology.
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