Comparison of the vitality tests used in the dental clinical practice and histological analysis of the dental pulp.

IF 3.4 4区 医学 Q2 MEDICINE, RESEARCH & EXPERIMENTAL
Ana Tenyi, Lidija Nemeth, Aljaž Golež, Ksenija Cankar, Aleksandra Milutinović
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引用次数: 2

Abstract

In dentistry, indirect diagnostic methods such as electrical sensibility testing and pulse oximetry are used to assess the status of the pulp. Our study aimed to determine the correlation between hemoglobin oxygen saturation and vascular volume density (Vvasc). We also wanted to examine an electrical sensibility test and the volume density of myelinated nerve fibers (Vnerv). Twenty-six intact permanent premolars were included in the study. For histological analysis, the pulp tissue was stained with hematoxylin-eosin and immunohistochemically for von Willebrand factor and S100 to detect blood vessels and myelinated nerve fibers, respectively. The stereological analysis was used to determine the Vvasc and Vnerv. Statistical analysis was done using the Pearson correlation test and Welch's ANOVA test. Histological analysis showed that the pulp tissue was strongly vascularized and innervated. A significant positive correlation was found between Vvasc and hemoglobin oxygen saturation levels (p=0.030). A significant negative correlation was found between Vnerv and the lowest electrical voltage that patient felt (p=0.033). According to the maturity of the dental apex, teeth were divided into a group with open (N=6, OA group) and closed apex (N=20, CA group). We found that pulps in the CA group had higher Vnerv than the OA group (p=0.037). In contrast, there were no significant differences in Vvasc of the pulp tissue (p=0.059), oxygen saturation (p=0.907), or electrical voltage (p=0.113) between both groups. We can conclude that the measurement of pulse oximetry and electrical sensibility test reflect the morphology of healthy pulp tissue independently of the maturity of the dental apex.

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牙科临床应用活力试验与牙髓组织学分析的比较。
在牙科,间接诊断方法,如电敏感性测试和脉搏血氧测定法被用来评估牙髓的状况。我们的研究旨在确定血红蛋白氧饱和度与血管体积密度(Vvasc)之间的相关性。我们还想检查电敏感性测试和髓鞘神经纤维(Vnerv)的体积密度。26颗完整的恒前臼齿被纳入研究。组织学分析采用苏木精-伊红染色,免疫组化检测血管性血友病因子,免疫组化检测S100检测血管和髓鞘神经纤维。采用立体分析方法测定Vvasc和Vnerv。统计学分析采用Pearson相关检验和Welch方差分析检验。组织学分析表明,牙髓组织血管丰富,神经支配。Vvasc与血红蛋白氧饱和度呈显著正相关(p=0.030)。Vnerv与患者感受到的最低电压呈显著负相关(p=0.033)。根据牙尖成熟程度分为开放牙尖组(N=6, OA组)和封闭牙尖组(N=20, CA组)。我们发现CA组的Vnerv高于OA组(p=0.037)。相比之下,两组在牙髓组织Vvasc (p=0.059)、氧饱和度(p=0.907)和电压(p=0.113)方面均无显著差异。由此可见,脉搏血氧测定和电敏试验反映了健康牙髓组织的形态,与牙尖的成熟度无关。
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来源期刊
Bosnian journal of basic medical sciences
Bosnian journal of basic medical sciences 医学-医学:研究与实验
CiteScore
7.40
自引率
5.90%
发文量
98
审稿时长
35 days
期刊介绍: The Bosnian Journal of Basic Medical Sciences (BJBMS) is an international, English-language, peer reviewed journal, publishing original articles from different disciplines of basic medical sciences. BJBMS welcomes original research and comprehensive reviews as well as short research communications in the field of biochemistry, genetics, immunology, microbiology, pathology, pharmacology, pharmaceutical sciences and physiology.
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