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In vitro Propagation of Vanda testacea (Lindl.) Reichb. F, a Medicinally Important Threatened Orchid Vanda testacea（Lindl.）Reichb的离体繁殖。F、 医学上重要的濒危兰花
Plant tissue culture & biotechnology Pub Date : 2022-01-10 DOI: 10.3329/ptcb.v31i2.57343
S. Kaur
{"title":"In vitro Propagation of Vanda testacea (Lindl.) Reichb. F, a Medicinally Important Threatened Orchid","authors":"S. Kaur","doi":"10.3329/ptcb.v31i2.57343","DOIUrl":"https://doi.org/10.3329/ptcb.v31i2.57343","url":null,"abstract":"The present study was planned to enable in vitro conservation of Vanda testacea, a highly medicinal orchid species through in vitro asymbiotic seed germination technique in Mitra orchid medium supplemented with cytokinins (Kn - 4.65 μM, BAP - 4.44 μM), and auxin (NAA- 5.37 μM). The germination frequency and initiation of germination was higher in NAA augmented medium and seedlings developed in 12.50 ± 0.50 weeks. Coconut water (20%) proved optimum for the multiplication of protocorm like bodies. Activated charcoal successfully checked the release of brownish exudates in the cultures.\u0000Plant Tissue Cult. & Biotech. 31(2): 153-160, 2021 (December)","PeriodicalId":91158,"journal":{"name":"Plant tissue culture & biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42591183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
In vitro Regeneration of Picralima nitida (Stapf). T. Durand & H. using Zygotic Embryo 镍皮云杉（Stapf）的体外再生。T.Durand和H.利用受精胚胎
Plant tissue culture & biotechnology Pub Date : 2022-01-10 DOI: 10.3329/ptcb.v31i2.57342
-. Kamdem, N. Donfagsiteli, Njoueretou Mfondi Mache, Carine Temegne Nono, Rodrigue Goimasse, Leila Zebaze Zambou, J. G. Nzweundji, E. Youmbi, L. B. Tonfack
{"title":"In vitro Regeneration of Picralima nitida (Stapf). T. Durand & H. using Zygotic Embryo","authors":"-. Kamdem, N. Donfagsiteli, Njoueretou Mfondi Mache, Carine Temegne Nono, Rodrigue Goimasse, Leila Zebaze Zambou, J. G. Nzweundji, E. Youmbi, L. B. Tonfack","doi":"10.3329/ptcb.v31i2.57342","DOIUrl":"https://doi.org/10.3329/ptcb.v31i2.57342","url":null,"abstract":"Disinfected mature seed embryos of Picralima nitida, were cultured in MS medium supplemented with different concentrations and combinations of 2,4-D, BAP and NAA to determine an efficient protocol for in vitro propagation. Nine culture media made of combination of different components were used in a factorial design with three replications. Results showed up to 80 ± 4% disinfection rate with combination of triton x- 100 (0.2%) and sodium hypochlorite (30%). Embryo germination was highest on control medium. Rooting was higher (2±1 roots per embryo) after 4 weeks on control medium and on BAP supplemented medium at 0.8 μM while the longest root (1.5±0.5 cm) was observed on 2,4-D supplemented medium at 1.8 μM. Black soil was suitable for leaf formation (4 ± 2 leaves) and shoot elongation (2±1 cm) after 8 weeks in acclimatisation. These results show efficient disinfection, regeneration and acclimatisation of Picralima nitida.\u0000Plant Tissue Cult. & Biotech. 31(2): 143-151, 2021 (December)","PeriodicalId":91158,"journal":{"name":"Plant tissue culture & biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44283415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development of an Efficient In vitro Regeneration Protocol for Chrysanthemum (Chrysanthemum morifolium Ramat) 菊花（菊花）高效体外再生方案的研制
Plant tissue culture & biotechnology Pub Date : 2022-01-10 DOI: 10.3329/ptcb.v31i2.57344
J. Chowdhury, M. Hoque, Rakha Hari Sarker
{"title":"Development of an Efficient In vitro Regeneration Protocol for Chrysanthemum (Chrysanthemum morifolium Ramat)","authors":"J. Chowdhury, M. Hoque, Rakha Hari Sarker","doi":"10.3329/ptcb.v31i2.57344","DOIUrl":"https://doi.org/10.3329/ptcb.v31i2.57344","url":null,"abstract":"An efficient and rapid in vitro regeneration protocol was developed for chrysanthemum (Chrysanthemum morifolium Ramat) using two local varieties of Bangladesh namely, BARI Chrysanthemum-2 (BARI Chry-2) and local yellow (Y). MS medium supplemented with nine different concentrations and combinations of BAP and IAA was employed to optimize regeneration protocol using young in vitro derived leaf explants. Direct organogenesis was observed from the leaf explants on MS medium supplemented with 0.5 mg/l BAP and 2.0 mg/l IAA (T6) for both the varieties. This treatment (T6) induced shoot buds directly on the adaxial surface of the leaf providing the highest regeneration percentage (90% for BARI Chry-2 and 94.73% for Y), the highest number of shoot/explant (7.6 for BARI Chry-2 and 8.6 for Y) and maximum length of the shoot after six weeks (3 cm for BARI Chry-2 and 2.9 cm for Y) of culture. Explants with initially regenerated shoots were subculture on hormone free MS medium for shoot elongation after 4 weeks of their inoculation. During elongation of shoots, 90-95% of the regenerated shoots produced roots spontaneously in hormone free MS medium within 7-8 weeks of their inoculation. Rooted plantlets were transplanted to the field following hardening where 100% plantlets were survived and produced flower without any variation.\u0000Plant Tissue Cult. & Biotech. 31(2): 161-171, 2021 (December)","PeriodicalId":91158,"journal":{"name":"Plant tissue culture & biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49082439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Rooting of the Endemic Brazilian Species Campomanesia pubescens using Biotechnological Techniques 巴西特有种短毛树的生物技术生根研究
Plant tissue culture & biotechnology Pub Date : 2022-01-10 DOI: 10.3329/ptcb.v31i2.57345
N. Campos, GJ Da Silva, R. Paiva
{"title":"Rooting of the Endemic Brazilian Species Campomanesia pubescens using Biotechnological Techniques","authors":"N. Campos, GJ Da Silva, R. Paiva","doi":"10.3329/ptcb.v31i2.57345","DOIUrl":"https://doi.org/10.3329/ptcb.v31i2.57345","url":null,"abstract":"Abstract not available\u0000Plant Tissue Cult. & Biotech. 31(2): 173-177, 2021 (December)","PeriodicalId":91158,"journal":{"name":"Plant tissue culture & biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41703149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In vitro Regeneration of Japanese Burdock (Arctium lappa L.) from Cotyledon and Leaf Derived Explants 日本牛蒡(Arctium lappa L.)子叶和叶片外植体离体再生研究
Plant tissue culture & biotechnology Pub Date : 2022-01-10 DOI: 10.3329/ptcb.v31i2.57340
Mustafa Abul Kalam Azad, M. Arifuzzaman, M. Hossain, Md Sohel Arman, M. N. Amin
{"title":"In vitro Regeneration of Japanese Burdock (Arctium lappa L.) from Cotyledon and Leaf Derived Explants","authors":"Mustafa Abul Kalam Azad, M. Arifuzzaman, M. Hossain, Md Sohel Arman, M. N. Amin","doi":"10.3329/ptcb.v31i2.57340","DOIUrl":"https://doi.org/10.3329/ptcb.v31i2.57340","url":null,"abstract":"Considering the vegetable and medicinal values, a micropropagation protocol has been established for Japanese Burdock (Arctium lappa L.) by culturing the explants of cotyledon and leaf obtained from in vitro grown seedlings. Direct shoot regeneration was achieved from cotyledon and leaf explants on MS fortified with 4.0 μM BAP and 2.0 μM IBA or NAA after 5 weeks of culture. In addition, both the explants also formed callus from their cut margins within 6 weeks of cultivation on medium complemented with 6.0 μM BAP and 4.0 μM IBA or NAA. Adventitious shoots were also redeveloped through indirect organogenesis from the cotyledon and leaf-derived callus within 10 weeks of culture on MS containing 4.0 μM BAP and 2.0 μM IBA or NAA. The highest rate of shoot reproduction was attained at the third subculture, and more than 12.6 shoots were formed per callus clump. Within 4 weeks of transfer to the rooting medium on MS containing 6.0 μM IBA, the cultured micro-shoots produced highest 5.3 roots per cultured shoot. Rooted plantlets were successfully established on a soil-composed-sand mixture under natural condition with 93.3% survival rate\u0000Plant Tissue Cult. & Biotech. 31(2): 123-134, 2021 (December)","PeriodicalId":91158,"journal":{"name":"Plant tissue culture & biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45969623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Indigenous Lipase Producing Bacteria for Lipid-rich Wastewater Treatment 原生脂肪酶产菌处理富脂废水
Plant tissue culture & biotechnology Pub Date : 2022-01-10 DOI: 10.3329/ptcb.v31i2.57341
Lovely Aktar, M. Moniruzzaman, Yasuzo Sakai, M. Saha
{"title":"Indigenous Lipase Producing Bacteria for Lipid-rich Wastewater Treatment","authors":"Lovely Aktar, M. Moniruzzaman, Yasuzo Sakai, M. Saha","doi":"10.3329/ptcb.v31i2.57341","DOIUrl":"https://doi.org/10.3329/ptcb.v31i2.57341","url":null,"abstract":"This study was undertaken to evaluate the removal of lipid-rich organic matter from wastewater by lipase producing bacteria. Ten potential lipase producing bacteria were isolated from lipid-rich environments in and around Dhaka Metropolitan city. Three of them produced lipase higher than 10 U/ml. These three isolates and their consortium were used for synthetic wastewater treatment in the laboratory. The initial COD value of synthetic wastewater was 1,200 mg/l. COD removal efficiencies in the synthetic wastewater were 74.75, 73.33 and 66.67% by the Stenotrophomonas maltophilia e-a22, Pseudomonas aeruginosa 12 and Bacillus subtilis 20B, respectively. Stenotrophomonas maltophilia showed better COD removal performance (74.75%) in case of monoculture. But consortium showed better COD removal (83.33%) than that of monoculture. Therefore, it could be concluded that consortium of three isolates will be more useful for wastewater treatment as seed cultures in the wastewater treatment plant associated with the lipid-rich wastewater.\u0000Plant Tissue Cult. & Biotech. 31(2): 135-142, 2021 (December)","PeriodicalId":91158,"journal":{"name":"Plant tissue culture & biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49103225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Micropropagation of Citrus indica Tanaka using Shoot tips from Mature Trees 利用成熟树梢尖进行田中柑桔的微繁
Plant tissue culture & biotechnology Pub Date : 2021-06-18 DOI: 10.3329/ptcb.v31i1.54107
Kiran Chhetri, B. Mathew, L. S. Pereira
{"title":"Micropropagation of Citrus indica Tanaka using Shoot tips from Mature Trees","authors":"Kiran Chhetri, B. Mathew, L. S. Pereira","doi":"10.3329/ptcb.v31i1.54107","DOIUrl":"https://doi.org/10.3329/ptcb.v31i1.54107","url":null,"abstract":"A study was conducted to standardize a protocol for in-vitro direct regeneration and mass multiplication of Citrus indica Tanaka using shoot tip explants excised from mature trees. Shoot tips were inoculated in MS medium supplemented with varying concentrations and combinations of cytokinins and auxins. MS media when fortified with BAP 0.5 mg/l and 0.5 mg/l BAP + 1.0 mg/l Kn were found to be the best treatments for shoot initiation while MS supplemented with 1 mg/l IBA; 0.5 mg/l NAA + 0.5 mg/l IBA and 0.5 mg/l NAA + 0.5 mg/l IAA were the best treatments for root induction. Among the different media used for hardening, 100 % survivability was obtained when plantlets were hardened using vermicompost as the potting medium. Subsequently, these plantlets were transferred to larger pots and acclimatization was achieved gradually in outdoor conditions. \u0000Plant Tissue Cult. & Biotech. 31(1): 13-23, 2021 (June)","PeriodicalId":91158,"journal":{"name":"Plant tissue culture & biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45144380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Agrobacterium-mediated Genetic Transformation of Rice var. BRRI Dhan 58 农杆菌介导的水稻品种BRRI Dhan 58的遗传转化
Plant tissue culture & biotechnology Pub Date : 2021-06-18 DOI: 10.3329/ptcb.v31i1.54113
M. S. A. Banu, B. Ahmed, S. Parveen, H. Rashid, K. M. K. Huda
{"title":"Agrobacterium-mediated Genetic Transformation of Rice var. BRRI Dhan 58","authors":"M. S. A. Banu, B. Ahmed, S. Parveen, H. Rashid, K. M. K. Huda","doi":"10.3329/ptcb.v31i1.54113","DOIUrl":"https://doi.org/10.3329/ptcb.v31i1.54113","url":null,"abstract":"Agrobacterium mediated genetic transformation of BRRI Dhan 58 was conducted by using immature embryos following indirect regeneration. High percentage of callus induction at 96.5% was obtained when seeds of BRRI dhan 58 were cultured on modified MS medium supplemented with 2.5 mg/l 2, 4-D under dark condition. The maximum regeneration response was rerecorded when MS was supplemented with 3 mg/l BAP + 0.5 mg/l NAA and 1.0 mg/l Kn. Genetic transformation was performed using A. tumefaciens strain LBA4404 harboring pCAMBIA1301 plasmid carrying the marker genes for β-glucuronidase (GUS) and hygromycin resistance (hptII). Integration of the GUS gene into the genome of the rice plants was confirmed by PCR. The leaf segments of the PCR positive transformed plants showed the expression of GUS. The results of this study would be an effective tool for crop improvement and functional studies of gene on rice plant. \u0000Plant Tissue Cult. & Biotech. 31(1): 71-80, 2021 (June)","PeriodicalId":91158,"journal":{"name":"Plant tissue culture & biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42975565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
In vitro Micropropagation of Pomegranate (Punica granatum L.) Derived from Cotyledon 石榴（Punica granatum L.）的离体微繁殖
Plant tissue culture & biotechnology Pub Date : 2021-06-18 DOI: 10.3329/ptcb.v31i1.54112
M. Kabir, P. Das, A. Mamun, Monirul Islam, A. Islam
{"title":"In vitro Micropropagation of Pomegranate (Punica granatum L.) Derived from Cotyledon","authors":"M. Kabir, P. Das, A. Mamun, Monirul Islam, A. Islam","doi":"10.3329/ptcb.v31i1.54112","DOIUrl":"https://doi.org/10.3329/ptcb.v31i1.54112","url":null,"abstract":"A high frequency in vitro plant regeneration of pomegranate was established on MS medium supplemented with different concentrations and combinations of plant growth regulators. As explant cotyledons were employed for this study. Ninety percent of the cultured explants responded to form shoots from 30 days old in vitro raised seedlings after 90 days of culture initiation in MS containing 1.0 mg/l IBA + 0.1 mg/l NAA. The average number of shoots per explant was 10.0 ± 2.20, shoot length of 12.0 ± 2.40 cm, node per regenerated shoot was 9.0 ± 1.60 and the leaf number was14.0 ± 1.40. Well developed shoots were cultured on half strength of MS medium supplemented with 0.5 mg/l IBA, in which 90% shoot induced roots implanted after one month. The average number of root per shoot was 8.0 ± 0.90 and the average root length of 6.5 ± 0.40 cm was observed in this medium. Eighty percent plantlets were survived in the outdoor condition during the acclimatization period of seven days. \u0000Plant Tissue Cult. & Biotech. 31(1): 61-69, 2021 (June)","PeriodicalId":91158,"journal":{"name":"Plant tissue culture & biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48602150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Genotypic Variability in Soybean [Glycine max (L.) Merrill] through Agrobacterium-Mediated Transformation 大豆Glycine max (L.)的基因型变异通过农杆菌介导的转化
Plant tissue culture & biotechnology Pub Date : 2020-12-11 DOI: 10.3329/PTCB.V30I2.50693
S. Shukla, A. Rani, Meeta Jain, Vineet Kumar
{"title":"Genotypic Variability in Soybean [Glycine max (L.) Merrill] through Agrobacterium-Mediated Transformation","authors":"S. Shukla, A. Rani, Meeta Jain, Vineet Kumar","doi":"10.3329/PTCB.V30I2.50693","DOIUrl":"https://doi.org/10.3329/PTCB.V30I2.50693","url":null,"abstract":"Embryonic tip explants of 92 Indian soyabean and 7 advanced breeding lines derived from soaked mature seeds were inoculated and co-cultivated for 5-day with Agrobacterium strain EHA105 carrying the binary vector pCambia1305.1 containing a hygromycin and kanamycin resistance gene as plant and bacterial selectable markers, respectively. Transient expression of transgene was monitored by histochemical localization of β-glucouronidase (GUSPlus) reporter activity in transformed ET tissues. A high genetic variability for Agrobacterium-infection ranging from 3.8 to 100% was observed in the form of transient GUS expression. Five highly efficient genotypes, namely DS-228, JS 335, JS 72-44, KHSb2, and JS 72-280 with transient GUS expression of 100, 98.1, 96.5, 96 and 92%, respectively were identified. In addition, various infectivity patterns in these genotypes were observed. Genotypes with very high transient GUS expression identified in this study may improve success rate of development of transgenic soybean. \u0000Plant Tissue Cult. & Biotech. 30(2): 231-242, 2020 (December)","PeriodicalId":91158,"journal":{"name":"Plant tissue culture & biotechnology","volume":"30 1","pages":"231-242"},"PeriodicalIF":0.0,"publicationDate":"2020-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47575826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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