In vitro Regeneration of Picralima nitida (Stapf). T. Durand & H. using Zygotic Embryo

Plant tissue culture & biotechnology Pub Date : 2022-01-10 DOI:10.3329/ptcb.v31i2.57342

-. Kamdem, N. Donfagsiteli, Njoueretou Mfondi Mache, Carine Temegne Nono, Rodrigue Goimasse, Leila Zebaze Zambou, J. G. Nzweundji, E. Youmbi, L. B. Tonfack

{"title":"In vitro Regeneration of Picralima nitida (Stapf). T. Durand & H. using Zygotic Embryo","authors":"-. Kamdem, N. Donfagsiteli, Njoueretou Mfondi Mache, Carine Temegne Nono, Rodrigue Goimasse, Leila Zebaze Zambou, J. G. Nzweundji, E. Youmbi, L. B. Tonfack","doi":"10.3329/ptcb.v31i2.57342","DOIUrl":null,"url":null,"abstract":"Disinfected mature seed embryos of Picralima nitida, were cultured in MS medium supplemented with different concentrations and combinations of 2,4-D, BAP and NAA to determine an efficient protocol for in vitro propagation. Nine culture media made of combination of different components were used in a factorial design with three replications. Results showed up to 80 ± 4% disinfection rate with combination of triton x- 100 (0.2%) and sodium hypochlorite (30%). Embryo germination was highest on control medium. Rooting was higher (2±1 roots per embryo) after 4 weeks on control medium and on BAP supplemented medium at 0.8 μM while the longest root (1.5±0.5 cm) was observed on 2,4-D supplemented medium at 1.8 μM. Black soil was suitable for leaf formation (4 ± 2 leaves) and shoot elongation (2±1 cm) after 8 weeks in acclimatisation. These results show efficient disinfection, regeneration and acclimatisation of Picralima nitida.\nPlant Tissue Cult. & Biotech. 31(2): 143-151, 2021 (December)","PeriodicalId":91158,"journal":{"name":"Plant tissue culture & biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2022-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant tissue culture & biotechnology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3329/ptcb.v31i2.57342","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

Abstract

Disinfected mature seed embryos of Picralima nitida, were cultured in MS medium supplemented with different concentrations and combinations of 2,4-D, BAP and NAA to determine an efficient protocol for in vitro propagation. Nine culture media made of combination of different components were used in a factorial design with three replications. Results showed up to 80 ± 4% disinfection rate with combination of triton x- 100 (0.2%) and sodium hypochlorite (30%). Embryo germination was highest on control medium. Rooting was higher (2±1 roots per embryo) after 4 weeks on control medium and on BAP supplemented medium at 0.8 μM while the longest root (1.5±0.5 cm) was observed on 2,4-D supplemented medium at 1.8 μM. Black soil was suitable for leaf formation (4 ± 2 leaves) and shoot elongation (2±1 cm) after 8 weeks in acclimatisation. These results show efficient disinfection, regeneration and acclimatisation of Picralima nitida. Plant Tissue Cult. & Biotech. 31(2): 143-151, 2021 (December)

查看原文本刊更多论文

镍皮云杉（Stapf）的体外再生。T.Durand和H.利用受精胚胎

在MS培养基中添加不同浓度的2,4- d、BAP和NAA，确定一种有效的离体繁殖方案。采用3个重复的因子设计，采用不同组分组合的9种培养基。结果表明:与次氯酸钠(30%)联合使用，灭菌率可达80±4%。对照培养基上胚萌发率最高。对照培养基和添加BAP的0.8 μM培养基培养4周后生根量最大(2±1根/胚)，而添加2,4- d的1.8 μM培养基培养4周后生根量最大(1.5±0.5 cm)。黑土在驯化8周后叶片形成(4±2片)和芽伸长(2±1 cm)最适宜。结果表明，该菌具有良好的消毒、再生和驯化效果。植物组织崇拜。与生物技术，31(2):143-151,2021 (12)

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Plant tissue culture & biotechnology

自引率

0.00%

发文量