Plant tissue culture & biotechnology最新文献

Development of an In vitro Propagation Protocol for Thalictrum foliolosum: An Endangered Medicinal Plant 濒危药用植物山楂离体繁殖方法的研究

Plant tissue culture & biotechnology Pub Date : 2023-06-22 DOI: 10.3329/ptcb.v33i1.66689

S. Rani, R. Puri, P. Boora, Abul Qasim, M. Mehta

{"title":"Development of an In vitro Propagation Protocol for Thalictrum foliolosum: An Endangered Medicinal Plant","authors":"S. Rani, R. Puri, P. Boora, Abul Qasim, M. Mehta","doi":"10.3329/ptcb.v33i1.66689","DOIUrl":"https://doi.org/10.3329/ptcb.v33i1.66689","url":null,"abstract":"The present study has developed a reproducible in vitro micropropagation protocol for endemic and endangered Thalictrum foliolosum by using leaf and root segments. Thalictrum foliolosum possesses high medicinal properties due to the presence of valuable phenols, flavonoids and alkaloids. The plants are uprooted due to their high medicinal and clinical applications, hence can lead to extinction threat. In vitro leaf and root, explant was used for induction of multiple shoots on MS medium supplemented with plant growth regulators like benzyl amino purine(BAP), indole-3- butyric acid (IBA), 1-Naphthalene acetic acid (NAA) and indole-3-acetic acid (IAA) alone or in combination. The best positive response for the formation of shoots from the leaf segment was obtained from MS media supplemented with BAP (1.0 mg/l) + NAA (0.6 mg/l) after 4 weeks and Maximum roots were formed on MS + IBA (0.6 mg/l) within 4-5 weeks. Likewise, the maximum percentage of shooting and rooting from root explant was obtained in MS + BAP (0.8 mg/l) + TDZ (0.4 mg/l) and MS + IAA (1.0 mg/l) + NAA (2.0 mg/l) after 4 weeks. The well-rooted acclimatized plantlets were successfully transferred to field conditions.\u0000Plant Tissue Cult. & Biotech. 33(1): 47-56, 2023 (June)","PeriodicalId":91158,"journal":{"name":"Plant tissue culture & biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46393040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Downregulation of the OsAKT1 K+/Na+ Transporter Gene by CRISPR-Cas9 Mediated Transformation in Sensitive Rice IR29 Makes it Tolerant to Salt Stress 通过CRISPR-Cas9介导的水稻IR29基因转化下调OsAKT1 K+/Na+转运体基因使其耐盐胁迫

Plant tissue culture & biotechnology Pub Date : 2023-06-22 DOI: 10.3329/ptcb.v33i1.66868

I. Khan, Aysha Akter Laboni, T. Azim, S. M. Elias, Z. I. Seraj

引用次数: 0

Phylogenetic Relationship among Grasses, Wheat and Pearl Millet using matK and rbcR Genes 利用matK和rbcR基因研究禾草、小麦和珍珠粟的系统发育关系

Plant tissue culture & biotechnology Pub Date : 2023-06-22 DOI: 10.3329/ptcb.v33i1.66904

M. Molla, M. Saha, D. Serba, S. Talukdar, M. Salam, M. Ahmed, M. S. Islam

{"title":"Phylogenetic Relationship among Grasses, Wheat and Pearl Millet using matK and rbcR Genes","authors":"M. Molla, M. Saha, D. Serba, S. Talukdar, M. Salam, M. Ahmed, M. S. Islam","doi":"10.3329/ptcb.v33i1.66904","DOIUrl":"https://doi.org/10.3329/ptcb.v33i1.66904","url":null,"abstract":"Fourteen genotypes of tall fescue, Brachypodium, wheat and pearl millet were used to find out phylogenetic relationships at their different ploidy level. Isolated chloroplast DNA of the 14 genotypes was sequenced using some selected matK and rbcR genetic markers. The matK and rbcR chloroplast gene sequences length were 1539bp and 1431bp, respectively. The consensus sequence length of matK gene was 1265bp in Brachypodium and wheat, 1266bp in both tall fescue Continental and Mditerranean species, 1269bp in pearl millet and for rbcR gene was only 1156bp in all studied four species. Except in Brachypodium, the variations of SNP for both matK and rbcR genes were observed in different ploidy levels of pearl millet and between the tall fescue Continental and Mediterranean species. Wheat with their different ploidy level gave the SNP variations only for matK gene. In respect of all ploidy levels, pearl millet had the highest number of SNP for both matK (247) and rbcR (130) genes. The tall fescue Continental (129) and Mediterranean (123) were in second position to show their genetic variation for matK gene. The rbcR gene had higher SNP at 5¢ regions of all four species, but matK gene only in wheat and tall fescue and 3¢ in millet and Brachypodium species. There was no in-del for rbcR gene, but the insertion of nucleotide for the matK gene was found highest in diploid wheat (11) and the deletion was in pearl millet (6). The phylogenetic analysis stated that their relationships were tall fescue Continental and tall fescue Mediterranean was much more related to wheat than pearl millet and Brachypodium.\u0000Plant Tissue Cult. & Biotech. 33(1): 57-70, 2023 (June)","PeriodicalId":91158,"journal":{"name":"Plant tissue culture & biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45009164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Agrobacterium-mediated Genetic Transformation of two Peanut (Arachis hypogaea L.) Varieties using Salinity Tolerant PDH45 Gene 农杆菌介导的两种花生的遗传转化耐盐PDH45基因的品种

Plant tissue culture & biotechnology Pub Date : 2023-06-22 DOI: 10.3329/ptcb.v33i1.66679

Ishita Haider, Rakha Hari Sarker, Md. Murshadul Hoque

{"title":"Agrobacterium-mediated Genetic Transformation of two Peanut (Arachis hypogaea L.) Varieties using Salinity Tolerant PDH45 Gene","authors":"Ishita Haider, Rakha Hari Sarker, Md. Murshadul Hoque","doi":"10.3329/ptcb.v33i1.66679","DOIUrl":"https://doi.org/10.3329/ptcb.v33i1.66679","url":null,"abstract":"An efficient Agrobacterium-mediated genetic transformation protocol has been developed in the present investigation to integrate salinity tolerant gene pea DNA helicase (PDH45) in two peanut varieties, Dhaka-1 and BARI Chinabadam-8. Decapitated half-embryo explants of the above-mentioned varieties were also used to develop a transformation compatible in vitro regeneration system. MS medium supplemented with 5.0 mg/l BAP and 0.5 mg/l Kn showed maximum response for shoot induction in both varieties. Half strength of MS medium supplemented with 0.2 mg/l IBA showed better response towards root induction for both varieties. Genetic transformation experiments were performed using Agrobacterium strain LBA4404 containing pCAMBIA1301-PDH45 conferring reporter gus gene, hygromycin resistance hpt gene, and salinity tolerant gene pdh45. The transformation variables were optimized, OD600 1.4 with 45 minutes of incubation period, and three days of co-culture showed the best performance. The transformed shoots were selected using 30 mg/l hygromycin. The overall transformation frequency was very low, where 0.10% shoots of Dhaka-1 and 0.07% shoots of BARI Chinabadam-8 survived the selection pressure. The stable integration of the transgene in the transformed shoots was confirmed through PCR analysis.\u0000Plant Tissue Cult. & Biotech. 33(1): 37-46, 2023 (June)","PeriodicalId":91158,"journal":{"name":"Plant tissue culture & biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44277542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro Regeneration of an Endangered Medicinal Orchid Dendrobium crepidatum Lindl. & Paxton through Protocorm like bodies. 濒危药用兰花石斛的离体再生。帕克斯顿通过原球茎状的身体。

Plant tissue culture & biotechnology Pub Date : 2023-06-22 DOI: 10.3329/ptcb.v33i1.66753

A. N. Pyati

引用次数: 0

Optimizing Micropropagation Protocol of Rose (Rosa hybrida L.) cv. ‘Double Delight’ 双喜玫瑰微繁殖技术的优化

Plant tissue culture & biotechnology Pub Date : 2023-06-22 DOI: 10.3329/ptcb.v33i1.66197

Kazi Tanbir Rahman, Snigdha Sarker, Md Raihan Iqbal Raju, M. T. Hossain

{"title":"Optimizing Micropropagation Protocol of Rose (Rosa hybrida L.) cv. ‘Double Delight’","authors":"Kazi Tanbir Rahman, Snigdha Sarker, Md Raihan Iqbal Raju, M. T. Hossain","doi":"10.3329/ptcb.v33i1.66197","DOIUrl":"https://doi.org/10.3329/ptcb.v33i1.66197","url":null,"abstract":"An experiment was conducted to develop a suitable in vitro plant regeneration technique for Rosa hybrida L. cv. ‘Double Delight’ using nodal segments and leaf tissues as explants through direct and indirect organogenesis. Aseptic explants were inoculated onto gelled MS medium contained various strengths of plant growth regulators alone and in combinations for callus and direct shoot induction. MS medium containing 2.0 mg/l BAP was found to be the most effective for direct shoot induction from nodal explants (87.5%) as it produced the maximum number of shoots per explant. The highest callus induction frequency was found to be 80% in leaf tissue, 72% in nodal segments and 96% in leaf and intermodal segments of in vitro raised plantlets in MS medium containing 2.5 mg/l 2, 4-D. The best percentage of indirect shoot organogenesis (93.33%) with maximum number and length of shoot were found when the different explant-derived callus was transferred in 3.0 mg/l BAP supplemented medium. The direct and indirectly induced shoots were multiplied on MS medium containing 3.0 mg/l BAP, where the average number and length of shoots per culture were 7.20 ± 0.80 and 5.22 ± 0.47 cm, respectively. Maximum rooting (80%) was observed in ½-strength of gelled MS medium containing 15.0 g/l sucrose with 1.0 mg/l IBA. Plantlets with the proper root system were then placed in a polybag with a 1:1:1 ratio of sand, garden soil, and compost, and they had a survival rate of about 76%.\u0000Plant Tissue Cult. & Biotech. 33(1): 25-36, 2023 (June)","PeriodicalId":91158,"journal":{"name":"Plant tissue culture & biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45612656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro Conservation and Exploiting Polyembryonate Potential of Synthetic Seeds of Malaxis acuminata D. Don 刺麻合成种子的体外保存及多胚潜能开发

Plant tissue culture & biotechnology Pub Date : 2023-06-22 DOI: 10.3329/ptcb.v33i1.66347

S. Kaur

引用次数: 0

Validation of a Multiplex PCR for the Simultaneous Detection of E. coli and Class 1 Integron 多重PCR同时检测大肠杆菌和1类整合子的验证

Plant tissue culture & biotechnology Pub Date : 2023-06-22 DOI: 10.3329/ptcb.v33i1.66063

Asif Hasan, Anindita Bhowmik, M. Islam, S. Ahsan

引用次数: 0

In vitro Regeneration of Chickpea (Cicer arietinum L.) cultivated in Bangladesh 孟加拉鹰嘴豆（Cicer arietinum L.）的体外再生

Plant tissue culture & biotechnology Pub Date : 2022-12-29 DOI: 10.3329/ptcb.v32i2.63554

Md Abdur Rauf Sarkar, Selim Sarkar, N. Akter, Shuraya Beente Rashid, S. Rahman

{"title":"In vitro Regeneration of Chickpea (Cicer arietinum L.) cultivated in Bangladesh","authors":"Md Abdur Rauf Sarkar, Selim Sarkar, N. Akter, Shuraya Beente Rashid, S. Rahman","doi":"10.3329/ptcb.v32i2.63554","DOIUrl":"https://doi.org/10.3329/ptcb.v32i2.63554","url":null,"abstract":"An efficient, and reproducible in vitro regeneration protocol was developed for three varieties of chickpea (Cicer arietinum L.) named Barichhola-9, Barichhola-10, and Barichhola-11 using embryos with decapitated root tips as explants. The best response towards callus induction was obtained on MS medium supplemented with a combination of 2.0 mg/l BAP and 0.3 mg/l NAA. MS medium containing 3.0 mg/l BAP alone provided the highest number of shoots from the induced callus. MS medium with 0.5 mg/l NAA was found to be the best for root induction from in vitro regenerated shoots in all three varieties of chickpea. In vitro regenerated well-rooted plantlets were transplanted into a small pot containing a mixture of soil and cocodust for acclimatization. Regeneration responses were found to be identical for the three varieties of chickpea used in this study. The results of the present investigation will facilitate the in vitro regeneration of chicpea varieties as well as further genetic improvement of this economically valuable crop species through genetic transformation.\u0000Plant Tissue Cult. & Biotech. 32(2): 205-216, 2022 (December)","PeriodicalId":91158,"journal":{"name":"Plant tissue culture & biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49150943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Indirect Shoot Organogenesis from the Leaf Explants of Oxalis corniculata (L.) - An Important Medicinal Plant 重要药用植物草叶外植体间接茎器官发生的研究

Plant tissue culture & biotechnology Pub Date : 2022-12-29 DOI: 10.3329/ptcb.v32i2.63552

V.N. Swetha Prasuna, C. N. Reddy, P. C. Lakshmi, M. Gopal, G. S. Sri Purna, B. Srinivas

引用次数: 0