Plant tissue culture & biotechnology最新文献_第10页

Effects of Carnation Essential Oil Extracted from Carnation Calli on Extending Shelf Life of Yoghurt 康乃馨愈伤组织精油对延长酸奶保质期的影响

Plant tissue culture & biotechnology Pub Date : 2019-06-26 DOI: 10.3329/PTCB.V29I1.41974

F. Assem, K. E. Shafei, Hoda S. El‐Sayed, M. Matter, M. Hanafy, A. Amer

{"title":"Effects of Carnation Essential Oil Extracted from Carnation Calli on Extending Shelf Life of Yoghurt","authors":"F. Assem, K. E. Shafei, Hoda S. El‐Sayed, M. Matter, M. Hanafy, A. Amer","doi":"10.3329/PTCB.V29I1.41974","DOIUrl":"https://doi.org/10.3329/PTCB.V29I1.41974","url":null,"abstract":"Carnation’s essential oil with high content of eugenol (monoterpenes) was extracted from carnation plant calli. Eugenol had antimicrobial effect and could be used to prolong the shelf life of fermented products as yoghurt. Eugenol in carnation essential oil was added to milk, at the percentages of (0.2, 0.4, 0.6, and 0.8 μl/ml milk, respectively before using the milk in the yoghurt manufacture. Results indicated that eugenol in carnation essential oil possessed good inhibitory effect against selected pathogenic bacterial strains at different concentrations, but had no inhibitory effect on Saccharomyces cerevisiae. Addition of carnation essential oil affected the pH and titratable acidity of the prepared yoghurt. Moreover, total solids, total protein and fat-to-dry matter records were slightly affected. The total viable counts, also, counts of yoghurt starter cultures and Lactobacillus acidophilus in yoghurt samples enhanced to become a maximum after 10 days of storage and reduced thereafter. Yeasts and molds, and coliform bacteria were not detected in the treated yoghurt. In different samples of yoghurt, yoghurt containing 0.6 μl/ml eugenol was the most acceptable organoleptically. It can be concluded that 0.6 μl/ml eugenol, can be applied to prolong yoghurt storage time for more than 15 days. \u0000Plant Tissue Cult. & Biotech. 29(1): 1-14, 2019 (June)","PeriodicalId":91158,"journal":{"name":"Plant tissue culture & biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3329/PTCB.V29I1.41974","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41432359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Recent Advances in Phalaenopsis Orchid Improvement using Omics Approaches 利用组学方法改良蝴蝶兰的研究进展

Plant tissue culture & biotechnology Pub Date : 2019-06-26 DOI: 10.3329/PTCB.V29I1.41986

Khosro Balilashaki, H. Zakizadeh, J. Olfati, M. Vahedi, Anuj Kumar, Meera Indracanti

引用次数: 4

Plant Regeneration in Buckwheat (Fagopyrum esculentum Moench.) via Somatic Embryogenesis and Induction of Meristemoids in Abnormal Embryos 荞麦（Fagopyrum esculentum Moench）体细胞胚胎发生和异常胚中分干细胞诱导的植株再生

Plant tissue culture & biotechnology Pub Date : 2019-06-26 DOI: 10.3329/PTCB.V29I1.41977

R. Saraswat, Mithilesh Kumar

{"title":"Plant Regeneration in Buckwheat (Fagopyrum esculentum Moench.) via Somatic Embryogenesis and Induction of Meristemoids in Abnormal Embryos","authors":"R. Saraswat, Mithilesh Kumar","doi":"10.3329/PTCB.V29I1.41977","DOIUrl":"https://doi.org/10.3329/PTCB.V29I1.41977","url":null,"abstract":"An efficient in vitro regeneration protocol is reported for common buckwheat. A combination of 0.5 mg/l 2,4-D and 0.2 mg/l BAP with sucrose showed highest induction of somatic embryogenesis from cotyledon and hypocotyl explants. More than 35% of normal somatic embryos matured on MS. MS with 2% sucrose were found best for germination and conversion of somatic embryos to plantlets. In tissue culture, abnormal somatic embryos usually occur. In this report, abnormal embryos are also used to induce shoot organogenesis, adding to the number of final regenerants and ensuring full utilization of regenerative propagules. A treatment of 0.2 mg/l BAP induced meristemoids in 60% of underdeveloped embryos and a combination of 0.5 mg/l BAP and 0.5 mg/l AgNO3 led browning and senescence-free progression of shoot buds to well developed shoots, which were subsequently rooted in half strength MS containing 2% sucrose and 0.25 mg/l IBA. The regenerated plants survived acclimatization, flowered and set seeds. \u0000Plant Tissue Cult. & Biotech. 29(1): 33-47, 2019 (June)","PeriodicalId":91158,"journal":{"name":"Plant tissue culture & biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3329/PTCB.V29I1.41977","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48455942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Prevention of Fungal Contamination in Plant Tissue Culture Using Cyclic Lipopeptides Secreted by Bacillus amyloliquefaciens AB30a 利用解淀粉芽孢杆菌AB30a分泌的环状脂肽预防植物组织培养中的真菌污染

Plant tissue culture & biotechnology Pub Date : 2019-06-26 DOI: 10.3329/PTCB.V29I1.41983

K. Rawal, H. Keharia

{"title":"Prevention of Fungal Contamination in Plant Tissue Culture Using Cyclic Lipopeptides Secreted by Bacillus amyloliquefaciens AB30a","authors":"K. Rawal, H. Keharia","doi":"10.3329/PTCB.V29I1.41983","DOIUrl":"https://doi.org/10.3329/PTCB.V29I1.41983","url":null,"abstract":"Plant tissue culture has revolutionized the field of plant biotechnology. However, there are certain obstacles which overall restrain the output of the plant tissue culturing. One of them is contamination of the tissue culture stock which is a major problem limiting the output. Aegle marmelos (L.) is a medicinal plant whose genotype qualities are maintained through clonal propagation of nodal segment as an explant. It harbors plethora of fungi which curbs the successful in vitro propagation. Chemical fungicide like bavistin is used to prevent the contamination in tissue culture which raises the environmental concerns. Thus, use of microbially derived antifungals can help in preventing fungal growth with benefit of positively impacting the plant growth. Here, authors investigated the use of heat stable lipopeptides which are secondary metabolites derived from Bacillus amyloliquefaciens AB30a for prevention of contamination in tissue culturing of nodal explants of A. marmelos positively impacting its in-vitro propagation. \u0000Plant Tissue Cult. & Biotech. 29(1): 111-119, 2019 (June)","PeriodicalId":91158,"journal":{"name":"Plant tissue culture & biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3329/PTCB.V29I1.41983","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46868832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Variability and Correlation Studies on Induced Mutants of Marigold (Tagetes erecta L.) for Different Traits and Assessing Them Using Molecular Markers 万寿菊不同性状诱导突变体的变异性、相关性研究及其分子标记评价

Plant tissue culture & biotechnology Pub Date : 2018-12-05 DOI: 10.3329/PTCB.V28I2.39681

J. Majumder, S. Singh, M. Kumari, M. Verma

{"title":"Variability and Correlation Studies on Induced Mutants of Marigold (Tagetes erecta L.) for Different Traits and Assessing Them Using Molecular Markers","authors":"J. Majumder, S. Singh, M. Kumari, M. Verma","doi":"10.3329/PTCB.V28I2.39681","DOIUrl":"https://doi.org/10.3329/PTCB.V28I2.39681","url":null,"abstract":"Induced mutations are important for creating novel variabilities in marigold for different purposes. Distinct putative mutants of marigold var. Pusa Narangi Gainda generated through gamma irradiation (in vivo and in vitro), were studied for variability and correlation in M2 generation for 11 traits. The maximum heritability (h2) was noted for days to flower harvest (0.9982%). The highest positive phenotypic correlation (0.637) was noted for number of flowers per plant to the days to first flower harvest. The most positive genotypic correlation (0.915) was found for seed yield (g) to the number of flowers per plant. Furthermore, the putative mutants in M2 were assessed for their diversity using ISSR markers and the amplified DNA fragments were compared with their respective parent genotype maintained (in vivo and in vitro). Single marker regression analysis indicated that the ISSR 811 and ISSR 817 were highly efficient markers for mutant identification in marigold. On the basis of dendrogram, putative mutant No. 3 (pm3) showed the highest dissimilarity with the parent (0.58). The mutants, pm5 and pm6 were found to be similar to each other (0.65), though they were quite distinct from the parent genotype morphologically. These selected mutants were multiplied up to six generations to attain the desired level of homozygosity for their large scale field evaluation. \u0000Plant Tissue Cult. & Biotech. 28(2): 223-236, 2018 (December)","PeriodicalId":91158,"journal":{"name":"Plant tissue culture & biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3329/PTCB.V28I2.39681","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47766530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro Plant Regeneration in Cleopatra (Citrus reshni Hort. ex Tan.) by Direct Organogenesis 克里奥帕特拉(埃及艳后)离体植株再生研究。例如Tan.)通过直接器官发生

Plant tissue culture & biotechnology Pub Date : 2018-12-05 DOI: 10.3329/PTCB.V28I2.39683

U. Devi, H. S. Rattanpal

引用次数: 0

Indirect in vitro Regeneration of Viola canescens Wall. ex, Roxb. by using Leaf Calli 藤黄离体间接再生的研究。前Roxb。通过使用叶卡利

Plant tissue culture & biotechnology Pub Date : 2018-12-05 DOI: 10.3329/PTCB.V28I2.39680

A. Khajuria, N. Bisht

引用次数: 2

Elicitation of Bacoside Content Using Plant Growth Regulators in Cell Suspension Culture of Bacopa monnieri (L.) Wettst. 利用植物生长调节剂在山豆芽悬浮培养中诱导Bacoside含量。

Plant tissue culture & biotechnology Pub Date : 2018-12-05 DOI: 10.3329/PTCB.V28I2.39678

Av Kharde, S. Kore, M. Khetmalas

引用次数: 7

Comparison of Genetic Variation of Anthurium (Anthurium andraeanum) Cultivars Using SCoT, CDDP and RAPD Markers 用SCoT、CDDP和RAPD标记比较红掌品种的遗传变异

Plant tissue culture & biotechnology Pub Date : 2018-12-05 DOI: 10.3329/PTCB.V28I2.39676

A. Saidi, Z. Daneshvar, Z. Hajibarat

{"title":"Comparison of Genetic Variation of Anthurium (Anthurium andraeanum) Cultivars Using SCoT, CDDP and RAPD Markers","authors":"A. Saidi, Z. Daneshvar, Z. Hajibarat","doi":"10.3329/PTCB.V28I2.39676","DOIUrl":"https://doi.org/10.3329/PTCB.V28I2.39676","url":null,"abstract":"To evaluate the genetic diversity among 10 cultivars of anthurium were performed using three molecular markers such as Start Codon Targeted (SCoT) and Conserved DNA-derived Polymorphism (CDDP), and Random Amplification of Polymorphic DNA (RAPD). Polymorphism index content (PIC) was calculated 0.39, 0.42 and 0.37 for RAPD, SCoT and CDDP, respectively. This result showed all the three molecular markers had almost an identical potential in estimating genetic diversity. Cluster analysis using SCoT, CDDP and RAPD divided the cultivars to three distinct clusters. The similarity matrix obtained through SCoT and CDDP was positively significantly correlated (r = 0.76, p < 0.01). This is the first report in which the efficiency of two targeted DNA region molecular markers (SCoT and CDDP) together with RAPD technique have been compared with each other in a set of anthurium cultivras. Results suggested that SCOT, CDDP and RAPD fingerprinting techniques are of sufficient ability to detect polymorphism in anthurium cultivars. \u0000Plant Tissue Cult. & Biotech. 28(2): 171-182, 2018 (December)","PeriodicalId":91158,"journal":{"name":"Plant tissue culture & biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3329/PTCB.V28I2.39676","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48381124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Efficient Adventitious Morphogenesis from In vitro Cultures of the Medicinal Plant Cymbopogon schoenanthus 药用植物杨桃离体培养的高效不定形态发生

Plant tissue culture & biotechnology Pub Date : 2018-12-05 DOI: 10.3329/PTCB.V28I2.39674

A. Abdel-Salam, K. Chowdhury, A. Bakry

引用次数: 6