M. Rahman, K. Jahan, Ashfak Ahmed Sabuz, A. Akanda
{"title":"Effects of Cucumber Mosaic Virus on Cellular Components, Host Physiology and Yield of Chilli","authors":"M. Rahman, K. Jahan, Ashfak Ahmed Sabuz, A. Akanda","doi":"10.3329/ptcb.v29i2.44511","DOIUrl":"https://doi.org/10.3329/ptcb.v29i2.44511","url":null,"abstract":"Alteration of cellular components and host physiology due to five biotypes of Cucumber mosaic virus (CMV) were investigated to know the effects on ultimate growth and yield of chilli. Severe reduction of cellular components like chlorophyll-a (59.31%), chlorophyll-b (68.40%), total chlorophyll (63.20%) and β-carotene (51.14%) were observed in plant infected by mosaic and stunting (MoS) as compared to healthy plant. The ratio of RNA to DNA was increased in the infected leaves. The reduction of photosynthesis rate was found 6.32 to 17.10% in CMV infected plants as compared to healthy plants. Stomatal conductance and inter cellular CO2 was found lower in CMV infected plats. Considerable yield reduction (28.80 to 78.11%.) was found infected with five symptomatic isolate of CMV. The present findings indicate that multiplication of virus particles in the infected cells alter the biochemical compounds of cells that disrupt the physiological process which affect the growth and yield of chilli.","PeriodicalId":91158,"journal":{"name":"Plant tissue culture & biotechnology","volume":"29 1","pages":"219-230"},"PeriodicalIF":0.0,"publicationDate":"2019-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3329/ptcb.v29i2.44511","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44111834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"In vitro Microtuber Induction and Regeneration of Plantlets from Microtuber Discs of Cultivated Potato (Solanum tuberosum L.)","authors":"R. Borna, M. Hoque, Rakha Hari Sarker","doi":"10.3329/PTCB.V29I1.41979","DOIUrl":"https://doi.org/10.3329/PTCB.V29I1.41979","url":null,"abstract":"In vitro microtuber induction potential of a popular potato (Solanum tuberosum L.) variety Diamant was examined using different supplements of growth regulators. Results showed that MS supplemented with 4.0 mg/l BAP, 1.0 mg/l IAA and 1.0 mg/l GA3 produced the best response towards microtuber induction from in vitro raised stem cuttings. Further, these in vitro raised microtubers were used to establish a highly efficient shoot regeneration system. Highest number of regenerated shoots were obtained from the peripheral region of microtuber disc explant on the MS containing 4.0 mg/l BAP and 2.0 mg/l IAA. Histological analysis revealed that microtuber periderm was composed of a several layers of uniform meristematic phellogen cells which were responded to growth hormones in the above mentioned medium producing healthy multiple shoots. Effective roots developed from these in vitro raised shoots on half strength of MS with 0.2 mg/l IBA. The plantlets were eventually acclimatized in pots containing soil for their establishment. \u0000Plant Tissue Cult. & Biotech. 29(1): 63-72, 2019 (June)","PeriodicalId":91158,"journal":{"name":"Plant tissue culture & biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3329/PTCB.V29I1.41979","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43168209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Isolation and Identification of Indigenous Poly-β-Hydroxybutyrate (PHB) Producing Bacteria from Different Waste Materials","authors":"F. Khan, Lovely Aktar, Tahmina Islam, M. Saha","doi":"10.3329/PTCB.V29I1.41975","DOIUrl":"https://doi.org/10.3329/PTCB.V29I1.41975","url":null,"abstract":"Attempt was made to isolate and identify poly-β-hydroxybutyrate (PHB) producing bacteria from different domestic and industrial waste materials from in and around Dhaka Metropolitan city. The bacterial load of the collected samples ranged in between 5.50 ×103 and 2.52 × 107, 3.10 × 104 and 2.23 × 107, 7.50 × 103 and 1.01 × 107 cfu/g or cfu/ml in NAG (nutrient agar glucose), PYG and LB media, respectively. The maximum bacterial count (2.52 × 107 cfu/g) was observed in NAG medium from soil sample of BDR market and the lowest bacterial load (5.5 × 103 cfu/ml) was observed in NAG medium from the garments wastewater. The highest number (33) of PHB producing bacteria was found in the kitchen waste soil and the lowest number (9) was in water sample of the Turag river. All the Sudan Black B positives isolates were tested for positive PHB production. Primarily, 160 PHB positive bacteria were isolated and finally 30 isolates were selected for identification. Provisionally identified potential 10 PHB positive bacterial isolates were identified on molecular basis. Nine of them were matched with their conventional identification but conventionally identified Rhizobium leguminosarum was found to be as Sinorhizobium sp. The isolated PHB producing bacteria could be used for biotechnological application. \u0000Plant Tissue Cult. & Biotech. 29(1): 15-24, 2019 (June)","PeriodicalId":91158,"journal":{"name":"Plant tissue culture & biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3329/PTCB.V29I1.41975","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48006144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. K. Bhajan, S. Begum, M. Islam, M. Hoque, Rakha Hari Sarker
{"title":"In vitro Regeneration and Agrobacterium-mediated Genetic Transformation of Local Varieties of Mungbean (Vigna radiata (L). Wilczek)","authors":"S. K. Bhajan, S. Begum, M. Islam, M. Hoque, Rakha Hari Sarker","doi":"10.3329/PTCB.V29I1.41981","DOIUrl":"https://doi.org/10.3329/PTCB.V29I1.41981","url":null,"abstract":"An efficient Agrobacterium-mediated transformation compatible in vitro regeneration protocol was developed for two important varieties of mungbean (Vigna radiata (L.) Wilczek) cultivated in Bangladesh, namely Binamoog-5 and BARI Mung-6. Two different zygotic embryo derived explants, such as cotyledonary node (CN) and cotyledon attached decapitated embryo (CADE) were used for direct organogenesis of shoot. MS supplemented with 4.0 μM BAP was found to be the best for the development of highest number of multiple shoots from CADE in both the varieties of mungbean. While in case CN the best shoot formation was achieved on MS containing 4.0 μM BAP and 0.5 μM NAA in both varieties. Half strength of MS with 2.0 μM IBA was found to be most effective for producing healthy root from regenerated shoots. Following root induction, the in vitro raised plantlets were successfully transplanted to soil for their establishment. Considering overall responses, genetic transformation efficiency was found to be better with CADE explant using Agrobacterium tumefaciens strain LBA4404 harboring the binary plasmid pBI121 conferring GUS and nptII genes. Different factors influencing transformation was optimized during this study. Selection of transformed shoots was carried out by gradually increasing the concentration of kanamycin and such transformed shoots were eventually selected using 200 mg/l kanamycin. Stable expression of the GUS gene was detected in various parts of regenerated transformed plantlets. Transformed shoots were rooted on half strength MS containing 2.0 μM IBA and 100 mg/l ticarcillin. Rooted transformed plantlets were successfully transferred to soil. Stable integration of GUS and nptII genes in the putative transformed shoots was confirmed through PCR analysis. \u0000Plant Tissue Cult. & Biotech. 29(1): 81-97, 2019 (June)","PeriodicalId":91158,"journal":{"name":"Plant tissue culture & biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3329/PTCB.V29I1.41981","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44798087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Paul, Tahmina Islam, Rakha Hari Sarker, M. Hoque
{"title":"In vitro Mass Propagation of Cymbidium aloifolium (L.) Sw.","authors":"M. Paul, Tahmina Islam, Rakha Hari Sarker, M. Hoque","doi":"10.3329/PTCB.V29I1.41980","DOIUrl":"https://doi.org/10.3329/PTCB.V29I1.41980","url":null,"abstract":"Cymbidium aloifolium (L.) Sw. is an epiphytic orchid distributed locally in Bangladesh. Natural populations of this orchid are under threat due to its high commercial value in the floriculture industry by over exploitation. Mass propagation provides an alternative means of satisfying the demand. In this study, half strength of MS supplemented with BAP or different combinations with NAA was evaluated for seed germination and early protocorm development of C. aloifolium (L.) Sw. The maximum percentage of seed germination (98) was obtained on modified MS (half strength of micro and macro nutrients and full strength of vitamin) supplemented with 0.5 mg/l BAP and 0.5 mg/l NAA after three weeks of culture. Maximum number of shoots (9.6 shoots per single shoot) were developed from shoot tip explants on half strength of MS supplemented with 1.0 mg/l BAP and 0.5 mg/l NAA. The most effective auxin source promoting root production (3.2 roots per shoot) was 1.0 mg/l IBA in combination with 0.5 mg/l BAP. The seedlings were successfully acclimatized (survival percentage 99) in a nethouse using a rooting medium of crushed sterile brick, cocopit and charcoal (1 : 1 : 1 v/v). \u0000Plant Tissue Cult. & Biotech. 29(1): 73-79, 2019 (June)","PeriodicalId":91158,"journal":{"name":"Plant tissue culture & biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3329/PTCB.V29I1.41980","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44363959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of 2, 4-D and Picloram on Somatic Embryogenesis in Carica papaya var. P-7-9","authors":"Kulbhushan Chaudhary, J. Prakash","doi":"10.3329/PTCB.V29I1.41976","DOIUrl":"https://doi.org/10.3329/PTCB.V29I1.41976","url":null,"abstract":"Immature zygotic embryos of Carica papaya L. var. P-7-9 were inoculated on half-strength MS with different concentrations of 2,4-D and picloram alone and their combinations. The highest induction (80%) of somatic embryogenesis was MS supplemented with 2,4-D (7.0 mg/l) followed by 2,4-D (4.0 mg/l) + picloram (1.0 mg/l) but no embryogenesis was observed in auxin-free medium. The addition of osmoticum such as PEG and ABA significantly increased the maturation of somatic embryos. BAP, TDZ and NAA were used for shoot induction. Combination of 2.0 mg/l BAP and 0.5 mg/l NAA was found significantly better for shoot bud proliferation and 70% multiplication. The percentage of germinating embryos was enhanced significantly when the MS fortified with GA3 (0.5 mg/l). However, the high frequency of embryo germination and plantlets formation were obtained with 2,4-D (4.0 mg/l) + picloram (1.0 mg/l). \u0000Plant Tissue Cult. & Biotech. 29(1): 25-32, 2019 (June)","PeriodicalId":91158,"journal":{"name":"Plant tissue culture & biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3329/PTCB.V29I1.41976","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41382493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Use of Chitosan as Low Cost Source to Overcome Endophytic Contaminations under In vitro Conditions","authors":"N. Shivakumar, M. Hegde","doi":"10.3329/PTCB.V29I1.41984","DOIUrl":"https://doi.org/10.3329/PTCB.V29I1.41984","url":null,"abstract":"Abstract not available \u0000Plant Tissue Cult. & Biotech. 29(1): 121-126, 2019 (June)","PeriodicalId":91158,"journal":{"name":"Plant tissue culture & biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3329/PTCB.V29I1.41984","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42353125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Subroto K Das, K. Shethi, M. Hoque, Rakha Hari Sarker
{"title":"Agrobacterium-mediated Genetic Transformation of Lentil (Lens culinaris Medik.) with Chitinase Gene followed by In vitro Flower and Pod Formation","authors":"Subroto K Das, K. Shethi, M. Hoque, Rakha Hari Sarker","doi":"10.3329/PTCB.V29I1.41982","DOIUrl":"https://doi.org/10.3329/PTCB.V29I1.41982","url":null,"abstract":"To investigate the integration of chitinase gene in lentil (Lens culinaris Medik.) namely, BARI masur-4 (BM-4), BARI masur-5 (BM-5) and BARI masur-6 (BM-6) through Agrobacterium-mediated genetic transformation was performed using Agrobacterium strain EHA 105 harboring bar (resistant to phosphinotrycin) and chitinase (gene of interest) gene. Selection of transformed shoots was carried out by gradually increasing the concentration of phosphinotrycin (PPT) up to 2.0 mg/l. Transgenic lentil shoots were produced with an overall frequency of 0.36 in case of BM-4 and BM-6 and 0.34 in case of BM-5, respectively. Most of the selected shoots developed in vitro flowers and pods following their sub-culture on half strength of MS supplemented with 20 mg/l IBA, 0.5 mg/l NAA with 50 mg/l ticarcillin. Seedlings germinated from the seeds were successfully transferred to soil for the development of further progeny. Stable integration of target gene was confirmed through PCR analysis. \u0000Plant Tissue Cult. & Biotech. 29(1): 99-109, 2019 (June)","PeriodicalId":91158,"journal":{"name":"Plant tissue culture & biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3329/PTCB.V29I1.41982","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46166571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"In vitro Selection of Drought Stress Tolerance in Rice (Oryza sativa L.) var. Jarava","authors":"Omar H. Obaid, S. Reddy","doi":"10.3329/PTCB.V29I1.41985","DOIUrl":"https://doi.org/10.3329/PTCB.V29I1.41985","url":null,"abstract":"Abstract not available \u0000Plant Tissue Cult. & Biotech. 29(1): 127-131, 2019 (June)","PeriodicalId":91158,"journal":{"name":"Plant tissue culture & biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3329/PTCB.V29I1.41985","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42711721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"In vitro Seed Germination, Protocorm Formation and Plantlet Regeneration in Aerides ringens Fisher","authors":"A. N. Pyati","doi":"10.3329/PTCB.V29I1.41978","DOIUrl":"https://doi.org/10.3329/PTCB.V29I1.41978","url":null,"abstract":"An attempt was made to in vitro seed germination and plantlet regeneration in Aerides ringens, a threatened, endemic and epiphytic orchid. Five basal media Knudson C (KC), Vacin and Went (VW), Burgeff’s (N3f), half strength MS, MS were examined for their effectiveness. Germination occurred in all the media. However, KC medium proved to be the best for seed germination (70.8%) and half strength MS was superior for the differentiation of protocorm like bodies (PLBs) into seedlings. The seeds were also cultured on various plant growth regulators (PGRs) to evaluate their effectiveness on seed germination and further differentiation. Among the PGRs tested, 0.57 μM IAA (IAA 89.3%), 4.64 μM Kn (88.6%) and 2.21 μM BAP (90.7%) and 0.57 μM IAA + 0.55 μM BAP (96.5%) were stimulated the germination and further differentiation of PLBs into seedlings. The concentrations of 2.21 μM BAP and combination of 0.57 μM IAA + 0.55 μM BAP stimulated the formation of MPLBs, which later differentiated into shoots and roots. Four potting substrates were evaluated for Ex vitro seedling survivability of which brick: charcoal: decaying litter: coconut husk (1 : 1 : 1 : 1) gave maximum survival rate (89.0%). Plant Tissue Cult. & Biotech. 29(1): 49-62, 2019 (June)","PeriodicalId":91158,"journal":{"name":"Plant tissue culture & biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3329/PTCB.V29I1.41978","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41699356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}