Plant tissue culture & biotechnology最新文献_第2页

Expression Pattern of in vitro Organogenesisassociated Genes as Transcriptional Marker in Sandalwood (Santalum album L.) Micropropagation 檀香体外器官发生相关基因转录标记的表达模式微体繁殖

Plant tissue culture & biotechnology Pub Date : 2022-12-29 DOI: 10.3329/ptcb.v32i2.63545

G. Pandey, G. Patil, Arpan Modi, S. Desai, Parth Desai, Subhash Narayanan

{"title":"Expression Pattern of in vitro Organogenesisassociated Genes as Transcriptional Marker in Sandalwood (Santalum album L.) Micropropagation","authors":"G. Pandey, G. Patil, Arpan Modi, S. Desai, Parth Desai, Subhash Narayanan","doi":"10.3329/ptcb.v32i2.63545","DOIUrl":"https://doi.org/10.3329/ptcb.v32i2.63545","url":null,"abstract":"The world's most expensive wood, sandalwood (Santalum album L.), requires a stringent mass-propagation technique to prevent future scarcity. Plant tissue culture is an efficient method that regenerates the whole plant from a single cell on a hormone-based growth medium. To efficiently regulate the formation of plants, it is important to understand the developmental organogenesis pathways (i.e., direct and indirect) through gene expression studies. Therefore, an effective protocol for the direct and indirect organogenesis of sandalwood was developed, followed by the characterization of gene expression patterns in investigation. Five in vitro organogenesis genes namely, Alternative oxidase (ao), Late embryogenesis abundant (lea), Cytochrome p450 (cyt-p450), ABC transporter (abct), and Serine-threonine phosphatase (stp), were screened from three stages of sandalwood development; (1) inoculated leaf, (2) proliferated leaf shoot/callus formation and (3) shoot formation. The best treatments for plant regeneration in Woody Plant Media (WPM) were SI24 (2.5 mg/l 2,4-D) for indirect organogenesis and SD14 (2.0 mg/l BAP and 0.4 mg/l-1 NAA) for direct organogenesis. During the initial stages of organogenesis, ao, cyt-p450 and abct showed no/little change in expression in the direct pathway however up-regulation of ao and abct and downregulation of cyt-p450 were observed in the indirect pathway. Expression of lea was increased up to 70-fold during direct and dropped to half during indirect organogenesis. The optimization of the sandalwood organogenesis regeneration medium and the identification of distinct gene expression patterns will serve as transcriptional markers for the early prediction of the organogenesis stage, assisting in the sandalwood conservation.\u0000Plant Tissue Cult. & Biotech. 32(2): 103-113, 2022 (December)","PeriodicalId":91158,"journal":{"name":"Plant tissue culture & biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42038125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morpho-Molecular Diversity of Cashew Nut (Anacardium occidentale L.) Germplasm of Bangladesh 腰果的形态与分子多样性孟加拉国种质资源

Plant tissue culture & biotechnology Pub Date : 2022-12-29 DOI: 10.3329/ptcb.v32i2.63551

Z. Sultana, S.M. Abdullah Al Mamun, A. Islam, Md. Abdul Mannan, Md. Monirul Islam

引用次数: 0

Genetic Diversity Analysis of Five Different Species of Riccia 五种不同Riccia的遗传多样性分析

Plant tissue culture & biotechnology Pub Date : 2022-12-29 DOI: 10.3329/ptcb.v32i2.63548

N. Tabassum, Mahbuba Jannat, M. Islam

引用次数: 0

Establishment of an Efficient in vitro Regeneration Protocol for Brassica campestris L. Var. Tori-7 甘蓝型油菜Tori-7高效离体再生体系的建立

Plant tissue culture & biotechnology Pub Date : 2022-12-29 DOI: 10.3329/ptcb.v32i2.63557

Tamanna Islam Toma, Ava Biswas, Saima Akhter, Tahmina Islam, M. Hoque, Rakha Hari Sarker

{"title":"Establishment of an Efficient in vitro Regeneration Protocol for Brassica campestris L. Var. Tori-7","authors":"Tamanna Islam Toma, Ava Biswas, Saima Akhter, Tahmina Islam, M. Hoque, Rakha Hari Sarker","doi":"10.3329/ptcb.v32i2.63557","DOIUrl":"https://doi.org/10.3329/ptcb.v32i2.63557","url":null,"abstract":"The aim of the present investigation was to develop an efficient and reproducible in vitro regeneration protocol for Brassica campestris L. var. Tori-7. Brassica is one of the most popular and major oil yielding crop plants around the world including Bangladesh. For in vitro regeneration of this crop plant, MS medium supplemented with various concentrations of 6-benzylaminopurine (BAP) and naphthalene acetic acid (NAA) were used. The highest frequency of callus and shoot regeneration was obtained on MS medium supplemented with 3.0 mg/l BAP and 0.2 mg/l NAA from both cotyledonary leaf with petiole and hypocotyl explants. Elongation of shoots was also optimized on the same media composition. For inducing roots from the excised in vitro derived shoots, half strength of MS medium supplemented with 0.3 mg/l indole-3-butyric acid (IBA) was found to be more effective than other hormonal supplements applied. Following the development of effective roots, in vitro raised plantlets were successfully transplanted into soil where they produced flowers and fertile seeds. The plants of R1 generation was successfully established in natural environment. In vitro regeneration protocol of Brassica campestris var. Tori-7 established in this study could be used for future plant genetic transformation experiments.\u0000Plant Tissue Cult. & Biotech. 32(2): 237-246, 2022 (December) ","PeriodicalId":91158,"journal":{"name":"Plant tissue culture & biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46800154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exopolysaccharide (EPS) Producing Bacteria of Sundarbans Mangrove Forest Soil 孙德尔本斯红树林土壤胞外多糖产生菌

Plant tissue culture & biotechnology Pub Date : 2022-12-29 DOI: 10.3329/ptcb.v32i2.63549

-. Zennathara, S. Afrin, Mohammad Ali, M. Islam, M. Saha

{"title":"Exopolysaccharide (EPS) Producing Bacteria of Sundarbans Mangrove Forest Soil","authors":"-. Zennathara, S. Afrin, Mohammad Ali, M. Islam, M. Saha","doi":"10.3329/ptcb.v32i2.63549","DOIUrl":"https://doi.org/10.3329/ptcb.v32i2.63549","url":null,"abstract":"The present study was undertaken to investigate the exopolysaccharide (EPS) producing bacteria from Sundarbans Mangrove Forest (SMF) soil of Bangladesh. The pH and aerobic heterotrophic bacterial counts of the soil samples ranged from 5.83 to 7.77 and 0.87 × 107 to 7.2 × 107 cfu/g, respectively. Potential 18 EPS producing bacterial isolates were selected for detailed study among which 15 were provisionally identified as members of the genus Bacillus, 2 were recognized as Dinococcus sp. and another one as Micrococcus sp. The genus Bacillus includes 7 distinct species viz. B. stearothermophilus, B. subtilis, B. brevis, B. marinus, B. schlegelli, B. pumilus and B. globisporous. The highest EPS production was found in the LB medium. The pHs of 6.5-7.5, 0% salinity, and 37°C temperature were found to be optimum for better growth of EPS producing bacteria. The three EPS producing bacterial isolates i.e. B. pumilus, B. globisporus and B. strearothermophilus were further confirmed through 16S rDNA sequence analysis. The culture and sensitivity (C/S) test results revealed streptomycin (S 10) as the most effective antibiotic to control the tested bacterial isolates.\u0000Plant Tissue Cult. & Biotech. 32(2): 145-156, 2022 (December)","PeriodicalId":91158,"journal":{"name":"Plant tissue culture & biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43558761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

In vitro Flowering and Production of Seeds in Oxalis corniculata L. - an Important Medicinal Plant 药用植物酢浆草的离体开花及种子生产

Plant tissue culture & biotechnology Pub Date : 2022-12-29 DOI: 10.3329/ptcb.v32i2.63553

V.N. Swetha Prasuna, C. N. Reddy, M. Rajagopal, G. S. Purna, B. Srinivas

引用次数: 0

Dye Decolorization and Organic Compounds Removal of Synthetic Textile Effluent (STE) by Anaerobic-aerobic Magnetic Activated Sludge (A2-MAS) Reactor 厌氧-好氧磁活性污泥（A2-MAS）反应器对合成纺织废水的脱色及有机物去除

Plant tissue culture & biotechnology Pub Date : 2022-12-29 DOI: 10.3329/ptcb.v32i2.63546

M. Saha, M. Oda, Yasuzo Sakai

{"title":"Dye Decolorization and Organic Compounds Removal of Synthetic Textile Effluent (STE) by Anaerobic-aerobic Magnetic Activated Sludge (A2-MAS) Reactor","authors":"M. Saha, M. Oda, Yasuzo Sakai","doi":"10.3329/ptcb.v32i2.63546","DOIUrl":"https://doi.org/10.3329/ptcb.v32i2.63546","url":null,"abstract":"The developed anaerobic-aerobic magnetic activated sludge (A2-MAS) reactor was found to be suitable for the textile effluent management. An azo dye (Orange II) and an anthraquinone dye (Remazol brilliant blue R) were used in the synthetic effluent (STE) for dye decolorization. The A2-MAS reactor consisted of an anaerobic tank (8.0 L) sequenced with an aerobic tank (4.0 L) for simultaneous dye decolorization and organic compounds removal. The anaerobic tank was operated at 35 ± 1°C while the aerobic part was maintained at room temperature. The magnetic separation technique in both aerobic and anaerobic condition of A2-MAS reactor was found effective and it is completely new to anaerobic operation. The sequential A2-MAS reactor attained well with 99.6% dye decolorization and 90.7% CODCr removal with the initial loading of 34.2 mg/l dye compounds and 365 mg/l CODCr, respectively. Anaerobic treatment played the major role for both dye decolorization (96.8%) and CODCr removal (78.4%) for the period of 100 day. Interestingly, sludge emission was zero all throughout the treatment.\u0000Plant Tissue Cult. & Biotech. 32(2): 115-125, 2022 (December)","PeriodicalId":91158,"journal":{"name":"Plant tissue culture & biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46529706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro Propagation of an Ornamental Bamboo (Bambusa tuldoides Munro) 观赏竹的离体繁殖研究

Plant tissue culture & biotechnology Pub Date : 2022-12-29 DOI: 10.3329/ptcb.v32i2.63550

Partho Sharothi, R. Raju, M. T. Hossain

{"title":"In vitro Propagation of an Ornamental Bamboo (Bambusa tuldoides Munro)","authors":"Partho Sharothi, R. Raju, M. T. Hossain","doi":"10.3329/ptcb.v32i2.63550","DOIUrl":"https://doi.org/10.3329/ptcb.v32i2.63550","url":null,"abstract":"This study was undertaken to standardize an efficient protocol for in vitro propagation of an ornamental bamboo Bambusa tuldoides Munro using nodal segments as explants. For direct shoot induction, liquid MS medium containing 2.0 mg/l BAP and 1.0 mg/l TDZ gave a maximum response (84%) with maximum number of shoots (4.80 ± 0.49) per explant. These shoots were proliferated efficiently on agitated liquid MS medium with 3.0 mg/l BAP and 1.5 mg/1 TDZ, which resulted in the formation of 15.40 ± 1.21 shoots per culture with 6.96 ± 0.65 cm shoot length. Shoots gradually increased in number and length during the first four subculture cycles in the same media combination but declined in 5th sub-culture cycle. After the 4th sub-culture cycle, the number of shoots per culture was 20.40 ± 1.44 and shoot length was 7.92 ± 0.78 cm. The addition of 10% coconut water with the above mentioned medium resulted in satisfactory shoot growth and development with 24.20 ± 1.16 shoots per culture. Maximum (84%) rooting with the highest number of roots per culture (7.20 ± 0.37) was obtained from half-strength MS medium fortified with 3.0 mg/l IBA. Well rooted plantlets were successfully acclimatized to the soil where the survival rate was 92%.\u0000Plant Tissue Cult. & Biotech. 32(2): 157-166, 2022 (December)","PeriodicalId":91158,"journal":{"name":"Plant tissue culture & biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42970131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro Micropropagation of Berberis chitria (Lindl.) - A Rare Medicinal Plant from Himachal Pradesh, India 印度喜马偕尔邦珍稀药用植物小檗离体微繁研究

Plant tissue culture & biotechnology Pub Date : 2022-12-29 DOI: 10.3329/ptcb.v32i2.63556

Shagun Sharma, H. Dhaliwal, V. Sharma

{"title":"In vitro Micropropagation of Berberis chitria (Lindl.) - A Rare Medicinal Plant from Himachal Pradesh, India","authors":"Shagun Sharma, H. Dhaliwal, V. Sharma","doi":"10.3329/ptcb.v32i2.63556","DOIUrl":"https://doi.org/10.3329/ptcb.v32i2.63556","url":null,"abstract":"An efficient in-vitro micropropagation protocol has been developed for Berberis chitria (Lindl.) - a rare medicinal plant from Himachal Pradesh, India using seeds and in-vitro nodal explants. The effects of different concentrations of BAP and GA3 for in-vitro seed germination was investigated and consequently 6.0 mg/l BAP + 0.0 mg/l GA3 was reported to be excellent for seed germination. Callus induction from leaf explants from aseptically grown seedling were also studied by using various concentrations of BAP and 2, 4-D and showing 94.26 ± 0.12% response at the concentration of 2.0 mg/l BAP + 0.4 mg/l 2, 4-D. The highest percentage of multiple shoot induction was obtained (100.00 ± 0.01% with an average of 4.50 ± 0.3 shoots) on MS medium augmented with 6.0 mg/l BAP + 1.0 mg/l Kn + 1.0 mg/l IAA. Whereas, the combined concentration of 1.0 mg/l NAA + 1.0 mg/l IBA + 0.5 mg/l IAA showed highest (76.05 ± 0.09%) root induction with an average of 3.84 ± 0.27 roots per shoot. The survival rate of these plantlets under greenhouse condition was 85%. This research communication provides first record about the in-vitro multiplication of B. chitria, collected from Himachal Pradesh Himalayas of India.\u0000Plant Tissue Cult. & Biotech. 32(2): 227-236, 2022 (December)","PeriodicalId":91158,"journal":{"name":"Plant tissue culture & biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48412095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Indirect Organogenesis from in vitro Derived Leaf and Internodes of Coccinia cordifolia (L.) Cogn. - An Important Medicinal Climber 从离体提取的堇果球叶和节间间接器官发生一位重要的医学攀登者

Plant tissue culture & biotechnology Pub Date : 2022-12-29 DOI: 10.3329/ptcb.v32i2.63547

Md Taufiqur Rahman Sarkar, M. Alam

{"title":"Indirect Organogenesis from in vitro Derived Leaf and Internodes of Coccinia cordifolia (L.) Cogn. - An Important Medicinal Climber","authors":"Md Taufiqur Rahman Sarkar, M. Alam","doi":"10.3329/ptcb.v32i2.63547","DOIUrl":"https://doi.org/10.3329/ptcb.v32i2.63547","url":null,"abstract":"An efficient in vitro regeneration protocol was developed via callus from the explants of leaf and internode of Coccinia cordifolia (L.) Cogn., an important medicinal climber. Among the different combinations of auxins and cytokines used, BAP with NAA were found highly effective for induction of callus from in vitro derived leaf disc and internode explants. Maximum callus formation rates were recorded on MS with 1.0 mg/l BAP and 0.1 mg/l NAA for both leaf disc and internode explants. The best organogenesis via callus was obtained on MS with 2.0 mg/l BAP and 0.5 mg/l NAA. The highest numbers of shoots regenerated per culture were 6.5 ± 0.10 and 5.6 ± 0.55 for leaf disc and internode explants respectively and leaf discs was found to be better for indirect organogenesis. For root induction, half strength of MS medium supplemented with 0.1 mg/l IBA was found to be the most effective. The mean number of roots regenerated per shoot was 6.8 ± 0.10 with an average root length of 2.8 ± 0.20 cm. In vitro derived plantlets were acclimatized successfully. This protocol for in vitro regeneration can be utilized for the improvement of this climber through biotechnological methods.\u0000Plant Tissue Cult. & Biotech. 32(2): 127-136, 2022 (December)","PeriodicalId":91158,"journal":{"name":"Plant tissue culture & biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48077933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0