日本牛蒡(Arctium lappa L.)子叶和叶片外植体离体再生研究

Mustafa Abul Kalam Azad, M. Arifuzzaman, M. Hossain, Md Sohel Arman, M. N. Amin
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引用次数: 0

摘要

考虑到蔬菜和药用价值,通过培养离体培养的牛蒡子叶和叶片的外植体,建立了牛蒡(Arctium lappa L.)的微繁殖方案。培养5周后,子叶和叶片外植体在用4.0μM BAP和2.0μM IBA或NAA强化的MS上获得了直接的芽再生。此外,在添加6.0μM BAP和4.0μM IBA或NAA的培养基上,两个外植体在6周内也从其切缘形成了愈伤组织。在含有4.0μM BAP和2.0μM IBA或NAA的MS上培养10周内,子叶和叶片来源的愈伤组织也通过间接器官发生重新发育不定芽。在第三次继代时,芽的繁殖率最高,每个愈伤组织丛可形成12.6个以上的芽。在含有6.0μM IBA的MS生根培养基上,培养的微芽在4周内产生最高的5.3根。在自然条件下,在由土壤组成的沙子混合物上成功地建立了生根植株,成活率为93.3%生物技术。31(2):123-1342021(12月)
本文章由计算机程序翻译,如有差异,请以英文原文为准。
In vitro Regeneration of Japanese Burdock (Arctium lappa L.) from Cotyledon and Leaf Derived Explants
Considering the vegetable and medicinal values, a micropropagation protocol has been established for Japanese Burdock (Arctium lappa L.) by culturing the explants of cotyledon and leaf obtained from in vitro grown seedlings. Direct shoot regeneration was achieved from cotyledon and leaf explants on MS fortified with 4.0 μM BAP and 2.0 μM IBA or NAA after 5 weeks of culture. In addition, both the explants also formed callus from their cut margins within 6 weeks of cultivation on medium complemented with 6.0 μM BAP and 4.0 μM IBA or NAA. Adventitious shoots were also redeveloped through indirect organogenesis from the cotyledon and leaf-derived callus within 10 weeks of culture on MS containing 4.0 μM BAP and 2.0 μM IBA or NAA. The highest rate of shoot reproduction was attained at the third subculture, and more than 12.6 shoots were formed per callus clump. Within 4 weeks of transfer to the rooting medium on MS containing 6.0 μM IBA, the cultured micro-shoots produced highest 5.3 roots per cultured shoot. Rooted plantlets were successfully established on a soil-composed-sand mixture under natural condition with 93.3% survival rate Plant Tissue Cult. & Biotech. 31(2): 123-134, 2021 (December)
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