Bio-medical materials and engineering最新文献_第5页

Temperature dependence of the protective effect of pressurized dissolution of xenon gas during cold storage of cells. 细胞冷藏期间加压溶解氙气的保护作用与温度有关。

IF 1 4区医学

Bio-medical materials and engineering Pub Date : 2024-11-01 DOI: 10.3233/BME-240105

Kenshi Mimura, Rina Sakai, Kazuhiro Yoshida, Masanobu Ujihira

{"title":"Temperature dependence of the protective effect of pressurized dissolution of xenon gas during cold storage of cells.","authors":"Kenshi Mimura, Rina Sakai, Kazuhiro Yoshida, Masanobu Ujihira","doi":"10.3233/BME-240105","DOIUrl":"10.3233/BME-240105","url":null,"abstract":"Background: The preservation period afforded by cold storage of cells is short. However, the use of rare gases for cold storage as a means of extending the period of preservation would be highly beneficial.Objective: To examine the effect of temperature on the protective effect of cold storage of cells using pressurized dissolution of xenon gas, with particular focus on the inhibition of substance transport by viscosity.Methods: Human dermal fibroblast monolayers incubated in a culture dish for 48 h were used as a test sample, with culture medium used as a preservation solution. Samples were placed into a pressure-resistant vessel, which was pressurized with xenon gas at 0 or 0.5 MPa, and cells were stored at 0 to 5°C for 18 h. Cell activity was evaluated by tetrazolium salt assay. The viscosity of the medium under pressurization at each storage temperature was estimated.Results: The maximum protective effect against cell damage of cold storage with pressurized dissolution of xenon gas was observed at 4°C. An increase in estimated viscosity by pressurization was correlated with increased cell activity at 4°C.Conclusion: Analysis of the temperature dependence of the protective effect against cell damage of cold storage with pressurized dissolution of xenon gas revealed that the most effective temperature is 4°C. The data also suggest that increased viscosity due to pressurization plays a role in the protective effect.","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":" ","pages":"530-535"},"PeriodicalIF":1.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142139303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extract from Falcaria vulgaris loaded with exosomes for the treatment of hypertension in pregnant mice: In vitro and In vivo investigations. 含有外泌体的法桐提取物用于治疗怀孕小鼠的高血压：体外和体内研究。

IF 1 4区医学

Bio-medical materials and engineering Pub Date : 2024-11-01 DOI: 10.3233/BME-240053

Jing Chen, Huan Wang, Jing Zhu

{"title":"Extract from Falcaria vulgaris loaded with exosomes for the treatment of hypertension in pregnant mice: In vitro and In vivo investigations.","authors":"Jing Chen, Huan Wang, Jing Zhu","doi":"10.3233/BME-240053","DOIUrl":"10.3233/BME-240053","url":null,"abstract":"Background: Hypertensive disorders during pregnancy pose significant risks to both maternal and fetal health, necessitating safe and effective therapeutic interventions.Objective: This study aimed to investigate the potential of an extract derived from Falcaria vulgaris (FV), loaded with exosomes to form the Exo/FV complex, as a novel therapeutic agent for the management of hypertension in pregnant mice: antioxidants, antimicrobials, and phenolic compounds present in FV lower blood pressure.Methods: The isolation of exosomes was done by ultracentrifugation methods and the FV was loaded into the exosomes by electroporation method.Results: The Exo/FV was found to be spherical with diameter ranges from 20 to 30 nm and they were tested for biocompatibility in NHI 3T3 cell lines and found to be effective. This research investigated in vivo hypertension in mice induced by L-NAME and treated with FV and Exo/FV and found that AChE and MAO determine mice's redox state tends to reduce blood pressure. Increased non-protein thiol (NP-SH) and decreased lipid peroxidation were also found, and PDE-5, ACE, Arginase, and MDA activity has also been tested.Conclusion: This analysis showed that Exo/FV effectively treated hypertension during pregnancy.","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":" ","pages":"509-521"},"PeriodicalIF":1.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142370960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SIKVAV promotion proliferation of vascular endothelial cells and related mechanisms. SIKVAV 促进血管内皮细胞增殖及其相关机制。

IF 1 4区医学

Bio-medical materials and engineering Pub Date : 2024-11-01 DOI: 10.3233/BME-240018

Xionglin Chen, Jie Zhang, Xiaoming Cao, He Jiang, Zhiren Wu, Zi du Zeng, Chen Jiang, Hui Chen

{"title":"SIKVAV promotion proliferation of vascular endothelial cells and related mechanisms.","authors":"Xionglin Chen, Jie Zhang, Xiaoming Cao, He Jiang, Zhiren Wu, Zi du Zeng, Chen Jiang, Hui Chen","doi":"10.3233/BME-240018","DOIUrl":"10.3233/BME-240018","url":null,"abstract":"Background: Vascular endothelial injury, a key factor in diabetic foot ulcers (DFUs) pathogenesis, is linked to the impaired proliferation and migration of vascular endothelial cells, modulated by hypoxia-inducible factor, growth factors, and inflammatory elements.Objective: The present study assesses the role of SIKVAV (Ser-Ile-Lys-Val-Ala-Val), a peptide shown to enhance cell proliferation and migration, on mouse aortic endothelial cell (MAEC) and the corresponding molecular mechanisms.Methods: MAEC were treated with SIKVAV at 0, 100, 200, 400, and 600 μg/mL for 0, 24, 48, and 72 h. Cell viability was tested using the CCK-8 assay. Proliferating cell nuclear antigen (PCNA), extracellular signal-regulated kinase 1/2 (ERK1/2), and protein kinase B (Akt) levels were measured by qRT-PCR and western blot.Results: SIKVAV augmented PCNA mRNA expression and stimulated vascular endothelial cell proliferation in a concentration and time-dependent fashion. Furthermore, it amplified the expression of p-ERK1/2 and p-Akt, pivotal components of the mitogen-activated protein kinase (MAPK)/ERK1/2 and phosphatidylinositol-3-kinase (PI3K)/Akt signaling pathways. The inhibition of these pathways suppressed PCNA mRNA expression, cell proliferation rate, and decreased p-ERK1/2 and p-Akt levels, highlighting SIKVAV's role in promoting vascular endothelial cell proliferation via these pathways.Conclusion: The results of this study confirmed that SIKVAV grafted onto scaffolds can accelerate the proliferation of vascular endothelial cells for the therapy of skin wounds, and provide a theoretical basis for its application in ischemic disease as synthesized biomaterials scaffolds of tissue engineering.","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":" ","pages":"499-508"},"PeriodicalIF":1.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142139302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Accuracy of an image matching technique for assessing knee alignment during the stance phase of gait using single-plane anteroposterior radiography. 利用单平面正后方放射摄影评估步态阶段膝关节对齐情况的图像匹配技术的准确性。

IF 1 4区医学

Bio-medical materials and engineering Pub Date : 2024-11-01 DOI: 10.3233/BME-240059

Koichi Kobayashi, Makoto Sakamoto, Tatsuya Soeno, Takashi Sato

{"title":"Accuracy of an image matching technique for assessing knee alignment during the stance phase of gait using single-plane anteroposterior radiography.","authors":"Koichi Kobayashi, Makoto Sakamoto, Tatsuya Soeno, Takashi Sato","doi":"10.3233/BME-240059","DOIUrl":"10.3233/BME-240059","url":null,"abstract":"Background: Accurate measurement of bone alignment of the knee during walking provides ideal clinical information for diagnosis and treatment of knee joint disorders. To bring this ideal closer to clinical reality, we developed an image matching technique to measure the three-dimensional (3D) position of bones using anteroposterior radiography during the stance phase of treadmill walking.Objective: To develop and validate an image matching method for evaluation of 3D knee alignment using anteroposterior radiography of artificial femoral and tibial bones.Methods: The 3D position of each bone was recovered by minimizing the difference between the projected outline and the contour of the bone in the anteroposterior radiograph. The true value of the position was measured using a 3D coordinate measuring machine.Results: The mean values ± standard deviation and root mean squares (RMS) of translation errors were within -1.6 ± 2.1 mm and 2.6 mm, respectively, for femur, and 2.1 ± 1.9 mm and 2.8 mm for tibia. The mean values ± standard deviation and RMS of errors in rotation were within 0.3 ± 0.7° and 0.7°, respectively, for femur, and -0.3 ± 0.9° and 0.9°, respectively, for tibia.Conclusion: Our method is suitable for evaluating 3D knee alignment on anteroposterior radiography.","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":" ","pages":"522-529"},"PeriodicalIF":1.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142035217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acupuncture navigation method integrated with augmented reality. 与增强现实技术相结合的针灸导航方法。

IF 1 4区医学

Bio-medical materials and engineering Pub Date : 2024-11-01 DOI: 10.3233/BME-240073

Shin-Yan Chiou, Meng-Ru He

引用次数: 0

RETRACTED: Mesenchymal stem cells derived from human adipose tissue exhibit significantly higher chondrogenic differentiation potential compared to those from rats. 与大鼠的间充质干细胞相比，从人体脂肪组织中提取的间充质干细胞具有更高的软骨分化潜能。

IF 1 4区医学

Bio-medical materials and engineering Pub Date : 2024-08-29 DOI: 10.3233/BME-240062

Faisal Yaqoob, Muhammad Khizer Hayat, Muhammad Sharjeel Chaughtai, Sehrish Khan, Musa Bin Bashir

引用次数: 0

Testing the shock protection performance of Type I construction helmets using impactors of different masses 使用不同质量的冲击器测试 I 型建筑安全帽的防震性能

IF 1 4区医学

Bio-medical materials and engineering Pub Date : 2024-04-23 DOI: 10.3233/bme-230173

John Z. Wu, Christopher S. Pan, Mahmood Ronaghi, Bryan M. Wimer

{"title":"Testing the shock protection performance of Type I construction helmets using impactors of different masses","authors":"John Z. Wu, Christopher S. Pan, Mahmood Ronaghi, Bryan M. Wimer","doi":"10.3233/bme-230173","DOIUrl":"https://doi.org/10.3233/bme-230173","url":null,"abstract":"BACKGROUND:Wearing protective helmets is an important prevention strategy to reduce work-related traumatic brain injuries. The existing standardized testing systems are used for quality control and do not provide a quantitative measure of the helmet performance. OBJECTIVE:To analyze the failure characterizations of Type I industrial helmets and develop a generalized approach to quantify the shock absorption performance of Type I industrial helmets based on the existing standardized setups. METHODS:A representative basic Type I construction helmet model was selected for the study. Top impact tests were performed on the helmets at different drop heights using two different impactor masses (3.6 and 5.0 kg). RESULTS:When the helmets were impacted with potential impact energies smaller than the critical potential impact energy values, there was a consistent relationship between the peak impact force and the potential impact energy. When the helmets were impacted under potential impact energies greater than the critical potential impact energy values, the peak impact forces increased steeply with increasing potential impact energy. CONCLUSION:A concept of safety margin for construction helmets based on potential impact energy was introduced to quantify the helmets’ shock absorption performance. The proposed method will help helmet manufacturers improve their product quality.","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":"11 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140799333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced treatment of acute organophosphorus pesticide poisoning using activated charcoal-embedded sodium alginate-polyvinyl alcohol hydrogel 使用嵌入活性炭的海藻酸钠-聚乙烯醇水凝胶加强对急性有机磷农药中毒的治疗

IF 1 4区医学

Bio-medical materials and engineering Pub Date : 2024-04-11 DOI: 10.3233/bme-240007

Li Yan, Ying Peng

{"title":"Enhanced treatment of acute organophosphorus pesticide poisoning using activated charcoal-embedded sodium alginate-polyvinyl alcohol hydrogel","authors":"Li Yan, Ying Peng","doi":"10.3233/bme-240007","DOIUrl":"https://doi.org/10.3233/bme-240007","url":null,"abstract":"BACKGROUND:The adsorption of activated charcoal is currently a major clinical treatment for acute organophosphorus pesticide poisoning (AOPP). However, the adsorption duration and efficiency of this method is unstable. OBJECTIVE:In this study, a hydrogel embedding activated charcoal was prepared and its alleviating effects on AOPP were investigated. METHODS:A composite hydrogel using sodium alginate and polyvinyl alcohol (SA-PVA) hydrogel was prepared in this study. The structural properties of the SA-PVA hydrogel were characterized via multiple analysis including FTIR, TGA, XRD, SEM, tensile strength and expansion rate. Based on these, activated charcoal (AC) was embedded within the SA-PVA hydrogel (SA-PVA-AC) and it was used for the treatment of AOPP. RESULTS:Structural characterization indicated SA-PVA hydrogel possesses excellent mechanical properties and biocompatibility. The in vivo study demonstrated that SA-PVA-AC significantly alleviated the inflammation and oxidative damage in the liver, as evidenced by reduced levels of IL-6, TNF-α, and, IL-1β, SOD, and MDA. Furthermore, SA-PVA-AC treatment effectively re-regulated the activities of serum AST and ALT, exhibiting an improved effect on liver function. CONCLUSION:The findings suggest that activated charcoal embedded within SA-PVA hydrogel has significant potential as a therapeutic agent in treating AOPP, and offering a novel approach to managing pesticide-induced toxicity.","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":"5 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2024-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140589165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

3D printed β-tricalcium phosphate versus synthetic bone mineral scaffolds: A comparative in vitro study of biocompatibility 三维打印β-磷酸三钙与合成骨矿物质支架：体外生物相容性比较研究

IF 1 4区医学

Bio-medical materials and engineering Pub Date : 2024-04-01 DOI: 10.3233/bme-230214

Blaire V. Slavin, Nicholas A. Mirsky, Zachary M. Stauber, Vasudev Vivekanand Nayak, James E. Smay, Cristobal F. Rivera, Dindo Q. Mijares, Paulo G. Coelho, Bruce N. Cronstein, Nick Tovar, Lukasz Witek

{"title":"3D printed β-tricalcium phosphate versus synthetic bone mineral scaffolds: A comparative in vitro study of biocompatibility","authors":"Blaire V. Slavin, Nicholas A. Mirsky, Zachary M. Stauber, Vasudev Vivekanand Nayak, James E. Smay, Cristobal F. Rivera, Dindo Q. Mijares, Paulo G. Coelho, Bruce N. Cronstein, Nick Tovar, Lukasz Witek","doi":"10.3233/bme-230214","DOIUrl":"https://doi.org/10.3233/bme-230214","url":null,"abstract":"BACKGROUND:β-tricalcium phosphate (β-TCP) has been successfully utilized as a 3D printed ceramic scaffold in the repair of non-healing bone defects; however, it requires the addition of growth factors to augment its regenerative capacity. Synthetic bone mineral (SBM) is a novel and extrudable carbonate hydroxyapatite with ionic substitutions known to facilitate bone healing. However, its efficacy as a 3D printed scaffold for hard tissue defect repair has not been explored. OBJECTIVE:To evaluate the biocompatibility and cell viability of human osteoprecursor (hOP) cells seeded on 3D printed SBM scaffolds via in vitro analysis. METHODS:SBM and β-TCP scaffolds were fabricated via 3D printing and sintered at various temperatures. Scaffolds were then subject to qualitative cytotoxicity testing and cell proliferation experiments utilizing (hOP) cells. RESULTS:SBM scaffolds sintered at lower temperatures (600 °C and 700 °C) induced greater levels of acute cellular stress. At higher sintering temperatures (1100 °C), SBM scaffolds showed inferior cellular viability relative to β-TCP scaffolds sintered to the same temperature (1100 °C). However, qualitative analysis suggested that β-TCP presented no evidence of morphological change, while SBM 1100 °C showed few instances of acute cellular stress. CONCLUSION:Results demonstrate SBM may be a promising alternative to β-TCP for potential applications in bone tissue engineering.","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":"22 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140589096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A hemodynamic model of artery bypass graft considering microcirculation function 考虑微循环功能的动脉旁路移植血液动力学模型

IF 1 4区医学

Bio-medical materials and engineering Pub Date : 2024-03-05 DOI: 10.3233/bme-230145

Fan He, Minru Li, Lu Hua, Tingting Guo

引用次数: 0