International journal of pharmaceutical sciences and research最新文献

{"title":"Suppressive Activity of Glucosamine Hydrochloride on Nitric Oxide Production from Synoviocytes In Vitro","authors":"C. Ohta, A. Yamada, Kuniko Yufune, K. Asano","doi":"10.15344/2394-1502/2016/111","DOIUrl":"https://doi.org/10.15344/2394-1502/2016/111","url":null,"abstract":"Background: Knee osteoarthritis (OA) is well known to be one of the most common joint disease in elderly people and characterized by a slowly progression of articular cartilage thinning and remodeling of the joints. Glucosamine hydrochloride (GH) and chondroitin sulfate, either alone or combined, are used for the treatment of OA and reported that these substances are favorably modify the clinical symptoms, especially pain, however, the underlying therapeutic mechanisms of these substances are not completely clear. The present study was undertaken to examine the influence of GH on the production of nitric oxide (NO), which is one of the important final effector molecules associated with OA, from synoviocytes from OA patients by an in vitro cell culture technique. Methods: Synoviocytes (1x105 cell/ml) were stimulated with 100.0 ng/ml periostin in the presence of various concentrations of GH. After 24 h, NO content in culture supernatants was examined by the Griess method. We also examined the influence of GH on transcription factor, NF-κB, activation and iNOS mRNA expression in synoviocytes 4 and 12 h after periostin stimulation, respectively. Results: Addition of GH into cell cultures caused the suppression of NO production from synoviocytes induced by periostin stimulation through the suppression of NF-κB activation and iNOS mRNA expression. The minimum concentration of GH that caused significant suppression of NO production was 1.5 mg/ml. Conclusion: These results strongly suggest that the ability of GH to suppress NO production from synoviocytes may account, at least in part, for the clinical efficacy of GH on OA.","PeriodicalId":90866,"journal":{"name":"International journal of pharmaceutical sciences and research","volume":"62 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88349647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationship between Abundance of Risk Factors and Prevalence of Asthma and Allergic Disease at South of Cairo","authors":"Melad Abdu Alhmed Al Dhduh, N. Sabri, E. Fouda","doi":"10.15344/2394-1502/2015/110","DOIUrl":"https://doi.org/10.15344/2394-1502/2015/110","url":null,"abstract":"Many studies have focused on environmental exposures among inner city subject with asthma, allergen exposure potentially leading to a higher prevalence of asthma and it has been suggested as among causes. In this study we investigate the relation between risk factors (exposure to air pollutions, family history and exposure to family pets or far animals), and asthma and other allergic disease exacerbation, and indicate the severity of like this diseases.","PeriodicalId":90866,"journal":{"name":"International journal of pharmaceutical sciences and research","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84822832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hypoglycemia Reported in Patient Safety Network (PSN)","authors":"S. Bughi, S. Fanchiang, B. Joyo, Richard Wong, A. Nazarian, Bryan Kakehashi, S. Shahinian, S. Desai, Paul Dillaway, T. Josic, Sylvia J. Shaw","doi":"10.15344/2394-1502/2015/109","DOIUrl":"https://doi.org/10.15344/2394-1502/2015/109","url":null,"abstract":"Hypoglycemia (hG) affects up to 26% of inpatients with diabetes mellitus (DM) and is responsible for an increase in cardiovascular morbidity and mortality. The goal of this pilot project (PP) was to decrease inpatient hG.","PeriodicalId":90866,"journal":{"name":"International journal of pharmaceutical sciences and research","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78316295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro Antioxidant Effect of the Leaf and Branch Extracts of Ribes L. Species in Turkey","authors":"G. Kendir, A. Köroğlu","doi":"10.15344/2394-1502/2015/108","DOIUrl":"https://doi.org/10.15344/2394-1502/2015/108","url":null,"abstract":"There are most studies on the antioxidant activity of Ribes nigrum and R. rubrum; however, this activity has been restricted on the other species. In the present study, the phenolic contents and antioxidant activities of different extracts prepared with methanol (MeOH), dichloromethane (DCM), ethyl acetate (EtOAc), n-buthanol (BuOH) and water from the leaf and branch of Ribes alpinum, R. anatolica, R. biebersteinii, R. multiflorum, R. nigrum, R.orientale, R.rubrum and R. uva-crispa, (Grossulariaceae) of Turkish origin were investigated by using in vitro methods.","PeriodicalId":90866,"journal":{"name":"International journal of pharmaceutical sciences and research","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80821439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Formulation and characterization of controlled release bioadhesive nanoparticles encapsulated with Neostigmine Bromide","authors":"Venkatesh Gavini, Srinivasa Rao Konijeti, T. Nagaraju","doi":"10.13040/IJPSR.0975-8232.6(8).3501-10","DOIUrl":"https://doi.org/10.13040/IJPSR.0975-8232.6(8).3501-10","url":null,"abstract":"In the recent days targeted drug delivery has gained more prominence for various advantages like site specific delivery and controlled release from the formulations. Amongst the plethora of avenues explored for targeted drug delivery, bioadhesive nanoparticles backed foremost attention offering local drug delivery and controlled drug release solving problems like tissue damage and drug wastage. Formulating nanoparticles with mucoadhesive polymers may provide a significant increase in the gastrointestinal residence time. Neostigmine bromide is a cholinesterase inhibitor used for the treatment of Myasthenia Gravis and is given by conventional routes like oral and intra venous. Bioadhesive nanoparticles of Neostigmine Bromide using synthetic and semi synthetic polymers like Carbopol, HPMC and ethyl cellulose were prepared by emulsification solvent evaporation method. The nanoparticles were characterized for their preformulation and post formulation parameters like compatibility, particle size, zeta potential, encapsulation efficiency, surface morphology, in vitro mucoadhesion, in vivo bioavailability, drug release and stability studies. Out of six, formulations F-1 and F-4 showed the best results for different evaluated parameters of nanoparticles. Entrapment efficiency was found to be within the range of 66.37% and 94.82%. Percentage mucoadhesion was within the range of 71.38% and 99.41%. In vitro dissolution was carried out for 10 hours and the percentage drug release for all the formulations were in the range of 98.93% and 89.71%. In vitro studies conclude that carbopol based nanoparticles are better than HPMC based nanoparticles for the delivery of Neostigmine Bromide. In vivo studies showed that the formulations F1 and F4 showed promising bioavailability compared to orally administered tablet.","PeriodicalId":90866,"journal":{"name":"International journal of pharmaceutical sciences and research","volume":"65 1","pages":"3501-3510"},"PeriodicalIF":0.0,"publicationDate":"2015-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80043473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and Severity of Allergic Diseases among Egyptian Pediatric in Different Egyptian Areas","authors":"Melad Abdu Alhmed Al Dhduh, N. Sabri, E. Fouda","doi":"10.15344/2394-1502/2015/107","DOIUrl":"https://doi.org/10.15344/2394-1502/2015/107","url":null,"abstract":"Allergic diseases have become one of the top three conditions demanding major effort toward prevention and control in the 21st century, according to the World Health Organization. Recently, the prevalence of asthma and allergic disease has increased annually throughout the world. It is not only affect the quality of life for children, but also creating a serious burden on all of society and family life.","PeriodicalId":90866,"journal":{"name":"International journal of pharmaceutical sciences and research","volume":"109 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84925713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical screening, cytotoxic and cns depressant activities of Holarrhena antidysenterica leaves and seeds","authors":"U. J. Nahar, M. R. Bhuiyan, A. N. M. Samsudduzah, M. Uddin, Z. Maryam","doi":"10.13040/ijpsr.0975-8232.6(2).620-23","DOIUrl":"https://doi.org/10.13040/ijpsr.0975-8232.6(2).620-23","url":null,"abstract":"Methanolic extract of leaves and seeds of Holarrhena antidysenterica was appraised individually to investigate the cytotoxic activity and CNS depressant activity of the leaf extract was observed adopting both open field test and hole cross test. Phytochemical screening with the leaf extract only, suggested the presence of alkaloids, cardiac glycoside, steroids, flavonoids, tannins and saponin. Brine shrimp lethality bioassay indicated significant cytotoxicity for both methanolic leaf and seed extract dissolved in DMSO (LC50 value 0.571 mu g/ml and 0.466 mu g/ml respectively) while LC50 value obtained by the standard, Vincristine Sulphate was 0.348 mu g/ml. Neuropharmacological experimental models in mice by hole cross and open field test indicated convincing CNS depressing activity of the leaf extract on Swiss albino mice at the dose of 100 mg/kg, 200 mg/kg and 300 mg/kg body weight whereas positive control Diazepam was used at a dose of 5mg/kg body weight. The validation of anxiety was carried out by measuring external signs, through hole-cross tests. Dose dependant activity was also identified by all the performed pharmacological investigations. Thus, this plant shows potential for further studies in future drug investigations.","PeriodicalId":90866,"journal":{"name":"International journal of pharmaceutical sciences and research","volume":"66 1","pages":"620-623"},"PeriodicalIF":0.0,"publicationDate":"2015-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86963775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Facts and Artifacts in the Evaluation of the Anti-diabetic Activity of Spent Hop Extract in Rat Hearts in the“Experimental Model of Diabetes”","authors":"M. Labieniec-Watala, T. Przygodzki, K. Siewiera, A. Podsędek, C. Watała","doi":"10.15344/2394-1502/2015/106","DOIUrl":"https://doi.org/10.15344/2394-1502/2015/106","url":null,"abstract":"The present study was undertaken to demonstrate the anti-diabetic mode of action of Humulus lupulus L. extract and its protective influence against changes in the respiratory capacity and coronary flow of the heart in experimental diabetes.","PeriodicalId":90866,"journal":{"name":"International journal of pharmaceutical sciences and research","volume":"9 1","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2015-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81686391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early prediction of cisplatin nephrotoxicity in head and neck cancer patients – An evaluation with urinary biomarkers","authors":"S. Ummer, K. Nalini, K. Gopalakrishna, R. Prabhu, Athiyamaan, A. Kamath, Vidyasagar","doi":"10.13040/IJPSR.0975-8232.6(7).2893-01","DOIUrl":"https://doi.org/10.13040/IJPSR.0975-8232.6(7).2893-01","url":null,"abstract":"Background: Nephrotoxic Acute Kidney Injury (AKI) is a common condition associated with considerable morbidity and mortality. Acute exposure to potentially nephrotoxic drugs requires early appraisal of the extent of renal injury to determine the need for specific interventions. Hence this study was designed to evaluate two urinary biomarkers for the early diagnosis of AKI due to cisplatin chemotherapy, including Neutrophil gelatinase-associated lipocalin (uNGAL) and Cystatin C (uCysC). Methods: Urinary biomarker levels in cisplatin treated cancer patients were estimated before and at 2hrs, 6hrs, 12hrs, 24hrs and 48hrs after cisplatin administration. Diagnostic performances of biomarkers were studied by ROC analysis with AUC and predictive values. Results: uNGAL was identified as the earlier biomarker of AKI induced by cisplatin as it detected kidney injury as early as 2hrs after the exposure of nephrotoxic drug with an AUC of 0.8, which is 46hrs before the elevation of serum creatinine. uCysC detected AKI 6hrs after cisplatin administration with AUC 0.73. Conclusion: Indication of AKI by biomarker elevations can provide an early warning signal which may have implications in therapy by either stopping the nephrotoxic drug or reducing its dose or even by substituting it with a less nephrotoxic one. The research in this direction should focus on evolving rapid procedures with an eventual point-of-care test for urinary biomarker determinations that will transform the scope of biomarkers significantly in the field of early renal injury.","PeriodicalId":90866,"journal":{"name":"International journal of pharmaceutical sciences and research","volume":"44 1","pages":"2893-2901"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77371714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In-vitro anti-cholinesterase activities by piperine, an alkaloid from the spice family Piperaceae","authors":"E. Okello, A. Coleman, C. Seal","doi":"10.13040/IJPSR.0975-8232.6(9).3726-32","DOIUrl":"https://doi.org/10.13040/IJPSR.0975-8232.6(9).3726-32","url":null,"abstract":"The alkaloid piperine from the spice family Piperaceae has been reported to possess poly-pharmacological activities including anti-depressant and cognitive enhancing effects. It has been suggested that its neurocognitive benefits may be via its activity on the cholinergic system, particularly on the enzyme acetylcholinesterase (AChE), a pharmacological target for neurodegenerative disease such as Alzheimer’s disease (AD). The paucity of information on the potential mechanism of inhibition of acetylcholinesterase and butyrylcholinesterase (BuChE), also a primary target for drug development for the treatment of AD, prompted this in vitro investigation. Dose-dependent inhibition of AChE and BuChE by piperine was determined using a modified classic colorimetric method of Ellman. Kinetics of inhibition was determined by Lineweaver-Burk methods. Piperine inhibited both AChE and BuChE in a concentration dependent manner with IC50 values of 0.12 mM and 0.067mM, respectively. Piperine exhibited a higher selectivity towards BuChE with a BuChE/AChE ratio of 0.56mM. Kinetic values for AChE classify piperine as a competitive inhibitor whereas the values for BuChE classify it as a mixed inhibitor. Compared to galanthamine (a mixed competitive non-competitive AChEinhibitor, IC50 of 1.068 nmol/ml under similar assay conditions) we conclude that although the AChE inhibition by piperine is not as potent as that of galanthamine, in addition to its known antioxidant and anti-inflammatory activities, piperine could provide a novel poly-pharmacological lead of potential benefit for the symptomatic treatment of AD and therefore warrants further investigation. INTRODUCTION: Alzheimer’s disease (AD) is generally recognised as the most prevalent form of dementia. It is an irreversible and progressive disease which destroys memory and cognitive skills and eventually leads to death. 1 AD is a multiaetiology disorder. Risk factors for AD include non-modifiable factors such as age and genetics, as well as modifiable factors such as dietary and lifestyle choices. 2 The current pharmacological options available for the treatment of AD include cholinesterase inhibition, glutamate receptor modulation, anti-oxidants and anti-inflammatory agents. 3","PeriodicalId":90866,"journal":{"name":"International journal of pharmaceutical sciences and research","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85311530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}