Biointerphases最新文献_第5页

Phenomenological investigation of organic modified cements as biocompatible substrates interfacing model marine organisms. 有机改性水泥作为生物相容性基质与海洋模式生物界面的现象学研究。

IF 1.9 4区医学

Biointerphases Pub Date : 2024-09-01 DOI: 10.1116/6.0003811

Jinglun Zhao, Tao Yuan, Hui Huang, Xiaolin Lu

引用次数: 0

Preparation and activity evaluation of zinc ion delivery system based on fucoidan-zinc complex. 基于褐藻素-锌复合物的锌离子输送系统的制备与活性评估

IF 1.9 4区医学

Biointerphases Pub Date : 2024-09-01 DOI: 10.1116/6.0003995

Zhongxiang Zhou, Yu Ding, Rui Cai, Changxu Ning, Jiangye Zhang, Xiuhan Guo

{"title":"Preparation and activity evaluation of zinc ion delivery system based on fucoidan-zinc complex.","authors":"Zhongxiang Zhou, Yu Ding, Rui Cai, Changxu Ning, Jiangye Zhang, Xiuhan Guo","doi":"10.1116/6.0003995","DOIUrl":"10.1116/6.0003995","url":null,"abstract":"Zinc is a critical trace element in the human body, playing a key role in regulating various protein functions and cellular metabolism. Thus, maintaining zinc homeostasis is essential for human health, as zinc deficiency can directly contribute to the onset of numerous diseases. Effective supplementation with zinc ions offers a viable treatment for zinc deficiency. Polysaccharides, particularly natural polysaccharides, exhibit extensive physiological activities and serve as efficient systems for delivering zinc ions. Fucoidan (F) is an affordable, widely available polysaccharide with significant bioactivity and safety, attracting growing research interest. However, most studies focus on its physiological functions, while few explore the structure and effects of fucoidan-metal complexes. In this study, fucoidan (F) was chosen to complex with Zn2+ to form the fucoidan-zinc (F-Zn) complex, whose structure was characterized. The zinc ion content reached 9.15%, with zinc (II) predominantly complexed with sulfate groups in the F-Zn (II) complex. Evaluation demonstrated that the prepared fucoidan-zinc system, at a concentration of 110 μg/ml, exhibited no significant cytotoxicity toward HT22 cells. Furthermore, both F and F-Zn exhibited significant neuroprotective effects in an HT22 cell model induced by cisplatin. Additional investigations revealed that F and F-Zn could mitigate cisplatin-induced increases in reactive oxygen species levels and alleviate mitochondrial damage. The fucoidan-zinc complex presents itself as a promising zinc ion delivery system for treating zinc deficiency.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"19 5","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142457690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of adhesion in the mechanics of pathogenic Listeria monocytogenes EGDe as a function of the pH of growth. 粘附力在致病性李斯特菌 EGDe 的力学中的作用与生长 pH 值的函数关系。

IF 1.9 4区医学

Biointerphases Pub Date : 2024-09-01 DOI: 10.1116/6.0003840

Asma Eskhan, Nehal I Abu-Lail

{"title":"Role of adhesion in the mechanics of pathogenic Listeria monocytogenes EGDe as a function of the pH of growth.","authors":"Asma Eskhan, Nehal I Abu-Lail","doi":"10.1116/6.0003840","DOIUrl":"10.1116/6.0003840","url":null,"abstract":"Atomic force microscopy was utilized to estimate the adhesion strengths to silicon nitride as well as the cellular elasticities of pathogenic Listeria monocytogenes EGDe cells cultured in media adjusted to five different pH conditions of growth (5, 6, 7, 8, and 9) under water with 0.0027 fixed ionic strength. Particularly, the role of adhesion on the bacterial elastic properties was investigated. The nonadhesive Hertz model of contact mechanics was used to extract Young's moduli of elasticity of bacterial cells from the approach force-indentation data. Additionally, the adhesive models of contact mechanics: Johnson-Kendall-Roberts (JKR) and Derjaguin-Muller-Toporov (DMT) were used to estimate Young's moduli of elasticity of bacterial cells from the retraction force-indentation data. Our results indicated that adhesion to silicon nitride was the highest for cells cultured at a pH of 7. Similarly, bacterial cells cultured at pH 7 were characterized by the highest Young's moduli of elasticities compared to the lower or higher pH conditions of growth. Young's moduli of elasticities estimated from the Hertz model were stiffer than those estimated using JKR or DMT models. As the adhesion between bacterial cells and indenters increased, the difference between the Hertz model and JKR or DMT models estimates of Young's moduli of elasticity increased as well. Contradicting the current norm of using the Hertz model to quantify bacterial elasticity in the literature, our results highlight the extreme importance of utilizing contact mechanics models with adhesion components in them such as the JKR and DMT models to estimate bacterial elasticity.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"19 5","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11501791/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142494847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adsorption of cytochrome c on different self-assembled monolayers: The role of surface chemistry and charge density. 细胞色素 c 在不同自组装单层上的吸附：表面化学和电荷密度的作用

IF 1.9 4区医学

Biointerphases Pub Date : 2024-09-01 DOI: 10.1116/6.0003986

Shengjiang Yang, Chunwang Peng, Jie Liu, Hai Yu, Zhiyong Xu, Yun Xie, Jian Zhou

{"title":"Adsorption of cytochrome c on different self-assembled monolayers: The role of surface chemistry and charge density.","authors":"Shengjiang Yang, Chunwang Peng, Jie Liu, Hai Yu, Zhiyong Xu, Yun Xie, Jian Zhou","doi":"10.1116/6.0003986","DOIUrl":"10.1116/6.0003986","url":null,"abstract":"In this work, the adsorption behavior of cytochrome c (Cyt-c) on five different self-assembled monolayers (SAMs) (i.e., CH3-SAM, OH-SAM, NH2-SAM, COOH-SAM, and OSO3--SAM) was studied by combined parallel tempering Monte Carlo and molecular dynamics simulations. The results show that Cyt-c binds to the CH3-SAM through a hydrophobic patch (especially Ile81) and undergoes a slight reorientation, while the adsorption on the OH-SAM is relatively weak. Cyt-c cannot stably bind to the lower surface charge density (SCD, 7% protonation) NH2-SAM even under a relatively high ionic strength condition, while a higher SCD of 25% protonation promotes Cyt-c adsorption on the NH2-SAM. The preferred adsorption orientations of Cyt-c on the negatively-charged surfaces are very similar, regardless of the surface chemistry and the SCD. As the SCD increases, more counterions are attracted to the charged surfaces, forming distinct counterion layers. The secondary structure of Cyt-c is well kept when adsorbed on these SAMs except the OSO3--SAM surface. The deactivation of redox properties for Cyt-c adsorbed on the highly negatively-charged surface is due to the confinement of heme reorientation and the farther position of the central iron to the surfaces, as well as the relatively larger conformation change of Cyt-c adsorbed on the OSO3--SAM surface. This work may provide insightful guidance for the design of Cyt-c-based bioelectronic devices and controlled enzyme immobilization.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"19 5","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142399300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adsorption of Staphylococcus aureus biofilm associated compounds on silica probed with molecular dynamics simulations. 利用分子动力学模拟探测金黄色葡萄球菌生物膜相关化合物在二氧化硅上的吸附。

IF 1.9 4区医学

Biointerphases Pub Date : 2024-09-01 DOI: 10.1116/6.0003870

Kelly M Lee, Vance W Jaeger

{"title":"Adsorption of Staphylococcus aureus biofilm associated compounds on silica probed with molecular dynamics simulations.","authors":"Kelly M Lee, Vance W Jaeger","doi":"10.1116/6.0003870","DOIUrl":"10.1116/6.0003870","url":null,"abstract":"Staphylococcus aureus (S. aureus) is a potentially pathogenic bacterium that commonly colonizes surfaces through the formation of biofilms. Silica glass is a common material in the built environment, especially in laboratory and medical spaces. The chemical and physical mechanisms by which S. aureus initially adheres to surfaces are unclear. In this study, the adsorption of several S. aureus biofilm associated compounds on silica is probed using molecular dynamics simulations. Model compounds containing a phosphorylated backbone, N-acetylglucosamine (GlcNAc), or D-alanine (D-Ala) were simulated across a range of pH. GlcNAc adsorption is unfavorable and insensitive to pH. D-Ala adsorption is unfavorable across the range of tested pH. Phosphorylated backbone adsorption is unfavorable at low pH but favorable at high pH. Adsorbate titration and solution salt concentration were probed to establish effects of molecular charge and charge screening. Hydrogen bonding between compounds and the silica surface is a key factor for stronger adsorption. The findings of this study are important for the rational design of improved silica surfaces through chemical functionalization or through the application of optimal chemical disinfectants that discourage the initial stages of biofilm growth.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"19 5","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142457689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamic spectroscopic and optical characterization and modeling of bovine serum albumin corona during interaction with N-hydroxysulfo-succinimide-covalently functionalized gold nanourchins. 牛血清白蛋白电晕与 N-hydroxysulfo-succinimide 共价功能化金纳米胆相互作用过程中的动态光谱和光学表征及建模。

IF 1.9 4区医学

Biointerphases Pub Date : 2024-09-01 DOI: 10.1116/6.0003715

Mohammad E Khosroshahi, Vaughan Woll-Morison, Kyungho Kim

{"title":"Dynamic spectroscopic and optical characterization and modeling of bovine serum albumin corona during interaction with N-hydroxysulfo-succinimide-covalently functionalized gold nanourchins.","authors":"Mohammad E Khosroshahi, Vaughan Woll-Morison, Kyungho Kim","doi":"10.1116/6.0003715","DOIUrl":"10.1116/6.0003715","url":null,"abstract":"In this study, bovine serum albumin (BSA) is used as a globular protein model to examine the conformational changes that occur during the interaction of BSA with N-hydroxysulfo-succinimide (sodium salt)-functionalized gold nanourchins (GNUs), for which dynamic spectroscopic techniques are employed. The results showed that the absorbance of phosphate-buffered saline-BSA at 278 nm decreased when a GNU was added to the solution due to adsorption, and it decreased further when the GNU was increased. The intensity and width of the peak of local surface plasmon resonance increased, indicating the effect of corona formation. Dynamic UV-vis spectroscopy and scattering revealed a nonlinear behavior of BSA-GNU interaction. The bioplasmonic solution resulted in higher transmission and scattering than the BSA solution. Fourier transform-near-infrared spectra exhibited several bands due to overtones and combinations of the amide group and different proportions of α-helix and β-sheet components in BSA before and after the addition of the GNU. Time-resolved fluorescence spectroscopy demonstrated an initial increase in blueshifted emission, followed by a redshifted quenching of two major peaks of Tyr and tryptophan (Trp). The binding and dissociation constants were determined as Kb = 2.17 × 1010 M-1 and Kd = 4.6 × 10-11, respectively, using the Stern-Volmer relation. Both the dynamic CMOS-based imaging and the cadmium sulfide sensors demonstrated a nonlinear response of bioplasmonic solution. By increasing the GNU, the resistance of the solution decreased in the order of A > S1 > S3, where S3 exhibited the highest initial transmission with a longer desorption time. MATLAB modeling showed 80% surface coverage by the protein in 15 s at 0.05M, equivalent to a thickness of 1.7 nm, which was in agreement with the value determined by using the Stokes-Einstein relation.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"19 5","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142361059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Organic photovoltaic biomaterial with fullerene derivatives for near-infrared light sensing in neural cells. 含富勒烯衍生物的有机光伏生物材料，用于神经细胞的近红外光传感。

IF 1.9 4区医学

Biointerphases Pub Date : 2024-07-01 DOI: 10.1116/6.0003279

Bowei Yuan, Xue Jiang, Zijun Xie, Xuanjun Zhang, Jiaxin Zhang, Jing Hong

{"title":"Organic photovoltaic biomaterial with fullerene derivatives for near-infrared light sensing in neural cells.","authors":"Bowei Yuan, Xue Jiang, Zijun Xie, Xuanjun Zhang, Jiaxin Zhang, Jing Hong","doi":"10.1116/6.0003279","DOIUrl":"10.1116/6.0003279","url":null,"abstract":"Retinal degenerative diseases, which can lead to photoreceptor cell apoptosis, have now become the leading irreversible cause of blindness worldwide. In this study, we developed an organic photovoltaic biomaterial for artificial retinas, enabling neural cells to detect photoelectric stimulation. The biomaterial was prepared using a conjugated polymer donor, PCE-10, and a non-fullerene receptor, Y6, both known for their strong near-infrared light absorption capabilities. Additionally, a fullerene receptor, PC61BM, was incorporated, which possesses the ability to absorb reactive oxygen species. We conducted a comprehensive investigation into the microstructure, photovoltaic properties, and photothermal effects of this three-component photovoltaic biomaterial. Furthermore, we employed Rat adrenal pheochromocytoma cells (PC-12) as a standard neural cell model to evaluate the in vitro photoelectric stimulation effect of this photovoltaic biomaterial. The results demonstrate that the photovoltaic biomaterial, enriched with fullerene derivatives, can induce intracellular calcium influx in PC-12 cells under 630 nm (red light) and 780 nm (near-infrared) laser irradiation. Moreover, there were lower levels of oxidative stress and higher levels of mitochondrial activity compared to the non-PC61BM group. This photovoltaic biomaterial proves to be an ideal substrate for near-infrared photoelectrical stimulation of neural cells and holds promise for restoring visual function in patients with photoreceptor apoptosis.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"19 4","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141615800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fighting against biofilm: The antifouling and antimicrobial material. 对抗生物膜：防污抗菌材料。

IF 1.9 4区医学

Biointerphases Pub Date : 2024-07-01 DOI: 10.1116/6.0003695

Chao Li, Dongdong Gao, Chunmei Li, Gang Cheng, Lijun Zhang

{"title":"Fighting against biofilm: The antifouling and antimicrobial material.","authors":"Chao Li, Dongdong Gao, Chunmei Li, Gang Cheng, Lijun Zhang","doi":"10.1116/6.0003695","DOIUrl":"10.1116/6.0003695","url":null,"abstract":"Biofilms are groups of microorganisms protected by self-secreted extracellular substances. Biofilm formation on the surface of biomaterial or engineering materials becomes a severe challenge. It has caused significant health, environmental, and societal concerns. It is believed that biofilms lead to life-threatening infection, medical implant failure, foodborne disease, and marine biofouling. To address these issues, tremendous effort has been made to inhibit biofilm formation on materials. Biofilms are extremely difficult to treat once formed, so designing material and coating bearing functional groups that are capable of resisting biofilm formation has attracted increasing attention for the last two decades. Many types of antibiofilm strategies have been designed to target different stages of biofilm formation. Development of the antibiofilm material can be classified into antifouling material, antimicrobial material, fouling release material, and integrated antifouling/antimicrobial material. This review summarizes relevant research utilizing these four approaches and comments on their antibiofilm properties. The feature of each method was compared to reveal the research trend. Antibiofilm strategies in fundamental research and industrial applications were summarized.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"19 4","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141632615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sequential release of transforming growth factor β1 and fibroblast growth factor 2 from nanofibrous scaffolds induces cartilage differentiation of mouse adipose-derived stem cells. 纳米纤维支架依次释放转化生长因子β1和成纤维细胞生长因子2可诱导小鼠脂肪来源干细胞的软骨分化。

IF 1.9 4区医学

Biointerphases Pub Date : 2024-07-01 DOI: 10.1116/6.0003687

Yun-Qi Wu, Jun Wang

{"title":"Sequential release of transforming growth factor β1 and fibroblast growth factor 2 from nanofibrous scaffolds induces cartilage differentiation of mouse adipose-derived stem cells.","authors":"Yun-Qi Wu, Jun Wang","doi":"10.1116/6.0003687","DOIUrl":"10.1116/6.0003687","url":null,"abstract":"Once damaged, cartilage has poor intrinsic capacity to repair itself. Current cartilage repair strategies cannot restore the damaged tissue sufficiently. It is hypothesized that biomimetic scaffolds, which can recapitulate important properties of the cartilage extracellular matrix, play a beneficial role in supporting cell behaviors such as growth, cartilage differentiation, and integration with native cartilage, ultimately facilitating tissue recovery. Adipose-derived stem cells regenerated cartilage upon the sequential release of transforming growth factor β1(TGFβ1) and fibroblast growth factor 2(FGF2) using a nanofibrous scaffold, in order to get the recovery of functional cartilage. Experiments in vitro have demonstrated that the release sequence of growth factors FGF2 to TGFβ1 is the most essential to promote adipose-derived stem cells into chondrocytes that then synthesize collagen II. Mouse subcutaneous implantation indicated that the treatment sequence of FGF2 to TGFβ1 was able to significantly induce multiple increase in cartilage regeneration in vivo. This result demonstrates that the group treated with FGF2 to TGFβ1 released from a nanofibrous scaffold provides a good strategy for cartilage regeneration by making a favorable microenvironment for cell growth and cartilage regeneration.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"19 4","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141757092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Building better habitats: Spatiotemporal signaling cues in 3D biointerfaces for tailored cellular functionality. 建设更好的栖息地：三维生物界面中的时空信号提示，实现量身定制的细胞功能。

IF 1.9 4区医学

Biointerphases Pub Date : 2024-07-01 DOI: 10.1116/6.0003685

Sadegh Ghorbani, Duncan S Sutherland

{"title":"Building better habitats: Spatiotemporal signaling cues in 3D biointerfaces for tailored cellular functionality.","authors":"Sadegh Ghorbani, Duncan S Sutherland","doi":"10.1116/6.0003685","DOIUrl":"10.1116/6.0003685","url":null,"abstract":"A promising research direction in the field of biological engineering is the design and functional programming of three-dimensional (3D) biointerfaces designed to support living cell functionality and growth in vitro, offering a route to precisely regulate cellular behaviors and phenotypes for addressing therapeutic challenges. While traditional two-dimensional (2D) biointerfaces have provided valuable insights, incorporating specific signaling cues into a 3D biointeractive microenvironment at the right locations and time is now recognized as crucial for accurately programming cellular decision-making and communication processes. This approach aims to engineer cell-centric microenvironments with the potential to recapitulate complex biological functions into a finite set of growing cellular organizations. Additionally, they provide insights into the hierarchical logic governing the relationship between molecular components and higher-order multicellular functionality. The functional live cell-based microenvironment engineered through such innovative biointerfaces has the potential to be used as an in vitro model system for expanding our understanding of cellular behaviors or as a therapeutic habitat where cellular functions can be reprogrammed.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"19 4","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141554170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0