Biointerphases最新文献_第6页

Influence of titanium and zirconia substrates on the synthesis of inflammatory mediators. 钛和氧化锆底物对炎症介质合成的影响。

IF 2.1 4区医学

Biointerphases Pub Date : 2023-07-01 DOI: 10.1116/6.0002659

João Moura Neto, Lais Medeiros Cardoso, Taisa Nogueira Pansani, Larissa M S C Raucci, Carlos Alberto de Souza Costa, Fernanda G Basso

{"title":"Influence of titanium and zirconia substrates on the synthesis of inflammatory mediators.","authors":"João Moura Neto, Lais Medeiros Cardoso, Taisa Nogueira Pansani, Larissa M S C Raucci, Carlos Alberto de Souza Costa, Fernanda G Basso","doi":"10.1116/6.0002659","DOIUrl":"https://doi.org/10.1116/6.0002659","url":null,"abstract":"The repair and homeostasis of peri-implant tissues depend on several factors such as the local presence of pathogenic bacteria and their products. Among other events, peri-implant tissue response is also related to the implant material used, which interferes with cells and extracellular matrix interactions, affecting the osseointegration process. In this study, the influence of zirconia (Zr) and titanium (Ti) substrates on the response of preosteoblasts (MC3T3) and murine macrophages (RAW 264.7) exposed to lipopolysaccharide (LPS, P. gingivalis) was evaluated. Zr and Ti disks were obtained and subjected to surface roughness standardization, which was analyzed by scanning electronic microscopy (SEM). The cells were subsequently cultured on Zr and Ti surfaces in AlphaMEM culture medium for 24 h, followed by LPS stimulus for 4 h. The production of reactive oxygen species (ROS) and gene expression of inflammatory markers were determined. SEM images showed that Ti disks exhibited higher surface roughness than that of Zr disks. Cells that seeded onto Ti and Zr had increased expression of inflammatory mediators and ROS production in the presence of LPS; however, such cell responses were more evident for Ti disks. These data indicate that contact of cells with Zr surfaces may lead to a lower inflammatory potential than Ti surfaces. Elucidation of the inflammatory response triggered by LPS for cells in contact with titanium and zirconia may contribute to the selection of materials for installation of osseointegrated implants.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"18 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10047138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Linking drivers of plant per- and polyfluoroalkyl substance (PFAS) uptake to agricultural land management decisions. 将植物全氟和多氟烷基物质(PFAS)吸收的驱动因素与农业土地管理决策联系起来。

IF 2.1 4区医学

Biointerphases Pub Date : 2023-07-01 DOI: 10.1116/6.0002772

Alex E Scearce, Caleb P Goossen, Rachel E Schattman, Ellen B Mallory, Jean D MaCrae

{"title":"Linking drivers of plant per- and polyfluoroalkyl substance (PFAS) uptake to agricultural land management decisions.","authors":"Alex E Scearce, Caleb P Goossen, Rachel E Schattman, Ellen B Mallory, Jean D MaCrae","doi":"10.1116/6.0002772","DOIUrl":"10.1116/6.0002772","url":null,"abstract":"Widespread contamination of the per- and polyfluoroalkyl substance (PFAS) in agricultural areas is largely attributed to the application of sewage sludge in which the PFAS can be concentrated. This creates a pathway for these contaminants to enter the food chain and, by extension, causes human health and economic concerns. One barrier to managing land with PFAS contamination is the variation in reported plant uptake levels across studies. A review of the literature suggests that the variation in plant uptake is influenced by a host of factors including the composition of PFAS chemicals, soil conditions, and plant physiology. Factors include (1) the chemical components of the PFAS such as the end group and chain length; (2) drivers of soil sorption such as the presence of soil organic matter (SOM), multivalent cation concentration, pH, soil type, and micropore volume; and (3) crop physiological features such as fine root area, percentage of mature roots, and leaf blade area. The wide range of driving factors highlights a need for research to elucidate these mechanisms through additional experiments as well as collect more data to support refined models capable of predicting PFAS uptake in a range of cropping systems. A conceptual framework presented here links drivers of plant PFAS uptake found in the literature to phytomanagement approaches such as modified agriculture or phytoremediation to provide decision support to land managers.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"18 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9860371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Ultraflexible polyvinylidene fluoride film based amperometric enzyme-free sensor for selective detection of uric acid in a trace level. 基于超柔性聚偏氟乙烯薄膜的无酶安培传感器，用于微量尿酸的选择性检测。

IF 2.1 4区医学

Biointerphases Pub Date : 2023-07-01 DOI: 10.1116/6.0002792

Abhisek Maikap, Riju Karmakar, Ajit Kumar Meikap, Subhra Samanta

{"title":"Ultraflexible polyvinylidene fluoride film based amperometric enzyme-free sensor for selective detection of uric acid in a trace level.","authors":"Abhisek Maikap, Riju Karmakar, Ajit Kumar Meikap, Subhra Samanta","doi":"10.1116/6.0002792","DOIUrl":"https://doi.org/10.1116/6.0002792","url":null,"abstract":"The present invention describes a novel flexible nanosensor for the electrochemical detection of uric acid (UA) present in urine. The synthesized graphite-boron nanocomposite with an average thickness of ∼32 nm was grown up on a flexible polyvinylidene fluoride film with an average thickness of ∼50 μm and it acts as a nonenzymatic sensor for UA. The developed flexible sensor showed a prominent reduction peak in cyclic voltammetry and amperometric response with the presence of different concentrations of aqueous UA solution. In the electrochemical study, the redox peak was generated near ∼-0.42 V with a detection limit of around ∼2.09 μM as the bottom level. The high robustness of the developed sensor originated from the polymeric film base and the rapid response time of ∼0.5 s for detecting UA present in human urine. The interference property of the sensor was confirmed in the presence of bilirubin and creatinine as an eventual reference toward selectivity. The phase and morphology of the sensor surface were extensively observed before and after sensing to comprehend the electrochemical interaction between the sensor and target molecules. The generated quantitative results of the integrated system were verified by testing known and unknown concentrations of UA solutions.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"18 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10114294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Theoretical study of electronic sum frequency generation spectroscopy to assess the buried interfaces. 电子和频产生谱评价埋藏界面的理论研究。

IF 2.1 4区医学

Biointerphases Pub Date : 2023-07-01 DOI: 10.1116/6.0002698

Yogesh Kumar, Suman Dhami, Ravindra Pandey

引用次数: 2

Immobilization of horseradish peroxidase with zwitterionic polymer material for industrial phenolic removal. 两性离子高分子材料固定化辣根过氧化物酶的工业苯酚脱除。

IF 2.1 4区医学

Biointerphases Pub Date : 2023-07-01 DOI: 10.1116/6.0002657

Qi Wang, Hao Fu, Xiaoyu Qi, Lei Zhang, Hongyan Ma

{"title":"Immobilization of horseradish peroxidase with zwitterionic polymer material for industrial phenolic removal.","authors":"Qi Wang, Hao Fu, Xiaoyu Qi, Lei Zhang, Hongyan Ma","doi":"10.1116/6.0002657","DOIUrl":"10.1116/6.0002657","url":null,"abstract":"Horseradish peroxidase (HRP) is a hemoglobin composed of a single peptide chain that catalyzes the oxidation of various substrates such as phenol and aniline in the presence of hydrogen peroxide via its iron-porphyrin catalytic center. This enzyme is widely used in industrial phenol removal, food additives, biomedicine, and clinical test reagents due to its rapid reaction rate and obvious reaction outcomes. However, the large-scale use of HRP in industrial applications still faces numerous challenges, including activity, stability, and sustainability. This study demonstrates that when peroxidase is immobilized in zwitterionic polymer hydrogels, polycarboxybetaine (PCB) and polysulfobetaine (PSB), the properties of the enzyme are improved. PCB and PSB-embedded HRP exhibit a 6.11 and 1.53 times increase in Kcat/Km value, respectively, compared to the free enzyme. The immobilized enzyme also experiences increased activity over a range of temperatures and better tolerance to extreme pH and organic solvents, including formaldehyde. In addition, immobilized HRP exhibits excellent performance in storage and reproducibility. Remarkably, PCB-HRP still retains 80% of the initial activity after a 6-week storage period and can still attain the initial catalytic level of the free enzyme after six repeated cycles. It also removes 90% of phenol within 12 min, surpassing the current pharmacy on the market. These experimental results indicated that we have successfully designed a set of stable and efficient support substrates for horseradish peroxidase, which enhances its suitability for deployment in industrial applications.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"18 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9805524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of electronic sum frequency generation spectrophotometer to assess the buried interfaces. 电子和频产生分光光度计的研制。

IF 2.1 4区医学

Biointerphases Pub Date : 2023-07-01 DOI: 10.1116/6.0002697

Suman Dhami, Yogesh Kumar, Ravindra Pandey

{"title":"Development of electronic sum frequency generation spectrophotometer to assess the buried interfaces.","authors":"Suman Dhami, Yogesh Kumar, Ravindra Pandey","doi":"10.1116/6.0002697","DOIUrl":"10.1116/6.0002697","url":null,"abstract":"The interfacial region between two bulk media in organic semiconductor based devices, such as organic field-effect transistors (OFETs), organic light-emitting diodes, and organic photovoltaics, refers to the region where two different materials such as an organic material and an electrode come in contact with each other. Although the interfacial region contains a significantly smaller fraction of molecules compared to the bulk, it is the primary site where many photoinduced excited state processes occur, such as charge transfer, charge recombination, separation, energy transfer processes, etc. All such photoinduced processes have a dependence on molecular orientation and density of states at the interfaces, therefore having an understanding of the interfacial region is essential. However, conventional spectroscopic techniques, such as surface-enhanced Raman scattering, x-ray photoelectron spectroscopy, atomic force microscopy, etc., face limitations in probing the orientation and density of states of interfacial molecules. Therefore, there is a need for noninvasive techniques capable of efficiently investigating the interfaces. The electronic sum frequency generation (ESFG) technique offers an interface selectivity based on the principle that the second-order nonlinear susceptibility tensor, within the electric dipole approximation, is zero in the isotropic bulk but nonzero at interfaces. This selectivity makes ESFG a promising spectroscopy tool to probe the molecular orientation and density of states at the buried interface. For beginners interested in employing ESFG to study the density of states at the interface, a detailed description of the experimental setup is provided here.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"18 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9804040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

PFAS (per- and polyfluoroalkyl substance)-free molded fiber: The future is already here. 无PFAS(全氟和多氟烷基物质)模制纤维:未来已经到来。

IF 2.1 4区医学

Biointerphases Pub Date : 2023-07-01 DOI: 10.1116/6.0002756

Mehdi Tajvidi

引用次数: 1

Evidence of compost contamination with per- and polyfluoroalkyl substances (PFAS) from "compostable" food serviceware. 来自“可堆肥”食品餐具的全氟和多氟烷基物质(PFAS)污染堆肥的证据。

IF 2.1 4区医学

Biointerphases Pub Date : 2023-05-01 DOI: 10.1116/6.0002746

Caleb P Goossen, Rachel E Schattman, Jean D MacRae

引用次数: 2

Back to the basics of time-of-flight secondary ion mass spectrometry data analysis of bio-related samples. II. Data processing and display. 生物相关样品的飞行时间二次离子质谱数据分析的基本原理。II.数据处理和显示。

IF 1.6 4区医学

Biointerphases Pub Date : 2023-05-01 DOI: 10.1116/6.0002633

Daniel J Graham, Lara J Gamble

引用次数: 0

Molecular simulation studies on a zwitterionic peptide-dendrimer conjugate for integrin αvβ3 binding. 两性离子肽-树状大分子结合整合素αvβ3的分子模拟研究。

IF 2.1 4区医学

Biointerphases Pub Date : 2023-05-01 DOI: 10.1116/6.0002713

Xiaowei Lin, Nan Xu, Chen Li, Zhiyu Wu, Shengfu Chen, Yao Shi, Yi He

{"title":"Molecular simulation studies on a zwitterionic peptide-dendrimer conjugate for integrin αvβ3 binding.","authors":"Xiaowei Lin, Nan Xu, Chen Li, Zhiyu Wu, Shengfu Chen, Yao Shi, Yi He","doi":"10.1116/6.0002713","DOIUrl":"https://doi.org/10.1116/6.0002713","url":null,"abstract":"Zwitterionic dendrimer is an effective carrier, which can restore the natural conformation of peptide segments for high bioaffinity by a hydrogen bond-induced conformational constraint approach. However, it is still unknown whether the approach is applicable for the dendrimers with different geometric sizes. Therefore, the characteristics of conjugates made from zwitterionic poly(amidoamine) (PAM) and the arginine-glycine-aspartic acid (RGD) peptide were examined to elucidate the effects of the geometric sizes of the PAM dendrimer on the conformational structure and stability of the peptide. The results show that the RGD fragments had almost the same structure and stability when conjugated with PAM(G3, G4, or G5) dendrimers. However, when conjugated with PAM(G1 or G2) dendrimers, the structural stability of these fragments was found to be much worse. Also, the structure and stability of RGD segments conjugated with PAM(G3, G4, or G5) were not affected when additional EK segments were inserted. Moreover, we observed that RGD fragments conjugated with PAM(G3, G4, or G5) dendrimers were structurally stable and similar when the concentration of NaCl was 0.15 and 0.5M. Furthermore, we show that PAM(G3, G4, or G5)-RGD conjugates bind strongly to integrin αvβ3.","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"18 3","pages":""},"PeriodicalIF":2.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9679422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0