ISRN biochemistry最新文献

Analysis of mathematical modelling on potentiometric biosensors. 电位生物传感器的数学建模分析。

ISRN biochemistry Pub Date : 2014-05-07 eCollection Date: 2014-01-01 DOI: 10.1155/2014/582675

N Mehala, L Rajendran

引用次数: 6

Tumor microenvironment: a new treatment target for cancer. 肿瘤微环境:癌症治疗的新靶点

ISRN biochemistry Pub Date : 2014-04-10 eCollection Date: 2014-01-01 DOI: 10.1155/2014/351959

Ming-Ju Tsai, Wei-An Chang, Ming-Shyan Huang, Po-Lin Kuo

引用次数: 111

New Lipase for Biodiesel Production: Partial Purification and Characterization of LipSB 25-4. 用于生物柴油生产的新型脂肪酶:LipSB 25-4的部分纯化与表征

ISRN biochemistry Pub Date : 2014-03-10 eCollection Date: 2014-01-01 DOI: 10.1155/2014/289749

Aysel Ugur, Nurdan Sarac, Rukiye Boran, Berk Ayaz, Ozgur Ceylan, Gulten Okmen

{"title":"New Lipase for Biodiesel Production: Partial Purification and Characterization of LipSB 25-4.","authors":"Aysel Ugur, Nurdan Sarac, Rukiye Boran, Berk Ayaz, Ozgur Ceylan, Gulten Okmen","doi":"10.1155/2014/289749","DOIUrl":"https://doi.org/10.1155/2014/289749","url":null,"abstract":"The lipolytic activities of 300 Streptomyces isolates were determined in Tributyrin and Rhodamine-B Agar. Lipase activities were also measured with p-nitrophenyl palmitate (p-NPP) as a substrate. The strain of Streptomyces bambergiensis OC 25-4 used in this study was selected among 300 strains of Streptomyces from MUCC as the best lipase producer. The incubation conditions were optimized and the inoculum amount, incubation period, effect of carbon and nitrogen sources, and rates of MgSO4 and CaCO3 were investigated. LipSB 25-4 (the lipase produced by S. bambergiensis OC 25-4 strain) was partially purified with ammonium sulphate precipitation, dialysis, and gel filtration chromatography 2.73-fold and with 92.12 U/mg specific activity. The optimal pH and temperature for LipSB 25-4 were determined as 8.0 and 50°C, respectively. The lipase has high stability in all pH and temperature values used in this study. While LipSB 25-4 was slightly activated in the presence of β-mercaptoethanol, it was slightly reduced by PMSF. The enzyme conserved approximately 75% of its activity at the end of 60 h, in the presence of methanol and ethanol. Since LipSB 25-4 displays high activity in the thermophilic conditions and stability in the presence of organic solvents, this lipase can catalyse the biodiesel production from olive oil by the transesterification reactions. ","PeriodicalId":90189,"journal":{"name":"ISRN biochemistry","volume":"2014 ","pages":"289749"},"PeriodicalIF":0.0,"publicationDate":"2014-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2014/289749","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33273614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 38

Synthetic melatoninergic ligands: achievements and prospects. 合成褪黑激素配体:进展与展望。

ISRN biochemistry Pub Date : 2014-02-23 eCollection Date: 2014-01-01 DOI: 10.1155/2014/843478

N V Kostiuk, M B Belyakova, D V Leshchenko, V V Zhigulina, M V Miniaev

引用次数: 5

Kinetic Characterization and Effect of Immobilized Thermostable β-Glucosidase in Alginate Gel Beads on Sugarcane Juice. 海藻酸盐凝胶珠固定化耐热β-葡萄糖苷酶对甘蔗汁的动力学表征及影响。

ISRN biochemistry Pub Date : 2014-02-20 eCollection Date: 2014-01-01 DOI: 10.1155/2014/178498

Keerti, Anuradha Gupta, Vinod Kumar, Ashutosh Dubey, A K Verma

{"title":"Kinetic Characterization and Effect of Immobilized Thermostable β-Glucosidase in Alginate Gel Beads on Sugarcane Juice.","authors":"Keerti, Anuradha Gupta, Vinod Kumar, Ashutosh Dubey, A K Verma","doi":"10.1155/2014/178498","DOIUrl":"https://doi.org/10.1155/2014/178498","url":null,"abstract":"A thermostable β-glucosidase was effectively immobilized on alginate by the method of gel entrapment. After optimization of immobilized conditions, recovered enzyme activity was 60%. Optimum pH, temperature, kinetic parameters, thermal and pH stability, reusability, and storage stability were investigated. The K m and V max for immobilized β-glucosidase were estimated to be 5.0 mM and 0.64 U/ml, respectively. When comparing, free and immobilized enzyme, change was observed in optimum pH and temperature from 5.0 to 6.0 and 60°C to 80°C, respectively. Immobilized enzyme showed an increase in pH stability over the studied pH range (3.0-10.0) and stability at temperature up to 80°C. The storage stability and reusability of the immobilized β-glucosidase were improved significantly, with 12.09% activity retention at 30°C after being stored for 25 d and 17.85% residual activity after being repeatedly used for 4 times. The effect of both free and immobilized β-glucosidase enzyme on physicochemical properties of sugarcane juice was also analyzed. ","PeriodicalId":90189,"journal":{"name":"ISRN biochemistry","volume":"2014 ","pages":"178498"},"PeriodicalIF":0.0,"publicationDate":"2014-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2014/178498","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33298677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 83

Transsulfuration Is a Significant Source of Sulfur for Glutathione Production in Human Mammary Epithelial Cells. 转硫是人类乳腺上皮细胞中产生谷胱甘肽的重要硫源。

ISRN biochemistry Pub Date : 2014-01-01 DOI: 10.1155/2013/637897

Andrea D Belalcázar, John G Ball, Leslie M Frost, Monica A Valentovic, John Wilkinson

{"title":"Transsulfuration Is a Significant Source of Sulfur for Glutathione Production in Human Mammary Epithelial Cells.","authors":"Andrea D Belalcázar, John G Ball, Leslie M Frost, Monica A Valentovic, John Wilkinson","doi":"10.1155/2013/637897","DOIUrl":"https://doi.org/10.1155/2013/637897","url":null,"abstract":"The transsulfuration pathway, through which homocysteine from the methionine cycle provides sulfur for cystathionine formation, which may subsequently be used for glutathione synthesis, has not heretofore been identified as active in mammary cells. Primary human mammary epithelial cells (HMEC's) were labeled with 35S-methionine for 24 hours following pretreatment with a vehicle control, the cysteine biosynthesis inhibitor propargylglycine or the gamma-glutamylcysteine synthesis inhibitor buthionine sulfoximine. Cell lysates were prepared and reacted with glutathione-S-transferase and the fluorescent labeling compound monochlorobimane to form a fluorescent glutathione-bimane conjugate. Comparison of fluorographic and autoradiographic images indicated that glutathione had incorporated 35S-methionine demonstrating that functional transsulfuration occurs in mammary cells. Pathway inhibitors reduced incorporation by roughly 80%. Measurement of glutathione production in HMEC's treated with and without hydrogen peroxide and/or pathway inhibitors indicates that the transsulfuration pathway plays a significant role in providing cysteine for glutathione production both normally and under conditions of oxidant stress.","PeriodicalId":90189,"journal":{"name":"ISRN biochemistry","volume":"2013 ","pages":"637897"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/637897","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32179817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 35

Production of Alkaline Protease by Solvent-Tolerant Alkaliphilic Bacillus circulans MTCC 7942 Isolated from Hydrocarbon Contaminated Habitat: Process Parameters Optimization. 从受碳氢化合物污染的生境中分离出来的耐溶剂嗜碱性芽孢杆菌 MTCC 7942 生产碱性蛋白酶：工艺参数优化。

ISRN biochemistry Pub Date : 2013-11-28 eCollection Date: 2013-01-01 DOI: 10.1155/2013/942590

Ulhas Patil, Ambalal Chaudhari

引用次数: 0

Variable-temperature size exclusion chromatography for the study of the structural changes in g-quadruplex. 变温粒径排除色谱法研究g-四联体的结构变化。

ISRN biochemistry Pub Date : 2013-11-10 eCollection Date: 2013-01-01 DOI: 10.1155/2013/631875

Sanae Benabou, Ramon Eritja, Raimundo Gargallo

引用次数: 3

Serum prolidase activity, oxidant and antioxidant status in nonulcer dyspepsia and healthy volunteers. 非溃疡性消化不良和健康志愿者血清脯氨酸酶活性、氧化剂和抗氧化状态的研究。

ISRN biochemistry Pub Date : 2013-10-29 eCollection Date: 2013-01-01 DOI: 10.1155/2013/182601

Shweta Kumari, Akhilesh Kumar Verma, Sumit Rungta, Rahul Mitra, Ragini Srivastava, Narender Kumar

{"title":"Serum prolidase activity, oxidant and antioxidant status in nonulcer dyspepsia and healthy volunteers.","authors":"Shweta Kumari, Akhilesh Kumar Verma, Sumit Rungta, Rahul Mitra, Ragini Srivastava, Narender Kumar","doi":"10.1155/2013/182601","DOIUrl":"https://doi.org/10.1155/2013/182601","url":null,"abstract":"Helicobacter pylori (H. pylori) infection is associated with increased oxidative stress and serum prolidase activity (SPA) in many diseases. We aimed to observe SPA and oxidative stress in nonulcer dyspepsia (NUD) infected with and without H. pylori among eastern Indians. 106 patients with H. pylori positive NUD, 82 patients with H. pylori negative NUD, and 50 healthy individuals were selected. SPA, total antioxidant capacity (TAOC), and total oxidant status (TOS) were measured with the use of spectrophotometer and an automated measurement method. SPA, TOS, and oxidative stress index (OSI) were significantly higher in patients with H. pylori positive than H. pylori negative NUD and healthy individuals (all P < 0.0001), whereas TAOC was significantly lower (P < 0.0001). Nonsignificant, increased SPA (P value = 0.6083) and decreased TAOC (P value = 0.1186) were observed in patients with H. pylori negative NUD than healthy individuals, while increased TOS and OSI were significant (P < 0.0001). Weak, nonsignificant correlations were observed between serum prolidase activity and TAOC, TOS, and OSI in H. pylori positive cases. Thus, increased SPA along with increased oxidative stress was observed, which seem to be closely associated with H. pylori infection. SPA and oxidative stress seem to be used as biomarkers for H. pylori infection in NUD. ","PeriodicalId":90189,"journal":{"name":"ISRN biochemistry","volume":"2013 ","pages":"182601"},"PeriodicalIF":0.0,"publicationDate":"2013-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/182601","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33272134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Evaluation of the In Vitro Efficacy of Artemisia annua, Rumex abyssinicus, and Catha edulis Forsk Extracts in Cancer and Trypanosoma brucei Cells. 黄花蒿、阿比西尼亚和长春花提取物对肿瘤和布鲁氏锥虫细胞体外抑制作用的研究。

ISRN biochemistry Pub Date : 2013-09-22 eCollection Date: 2013-01-01 DOI: 10.1155/2013/910308

Netsanet Worku, Andualem Mossie, August Stich, Arwid Daugschies, Susanne Trettner, Nasr Y A Hemdan, Gerd Birkenmeier

{"title":"Evaluation of the In Vitro Efficacy of Artemisia annua, Rumex abyssinicus, and Catha edulis Forsk Extracts in Cancer and Trypanosoma brucei Cells.","authors":"Netsanet Worku, Andualem Mossie, August Stich, Arwid Daugschies, Susanne Trettner, Nasr Y A Hemdan, Gerd Birkenmeier","doi":"10.1155/2013/910308","DOIUrl":"https://doi.org/10.1155/2013/910308","url":null,"abstract":"The current drugs against sleeping sickness are derived from cancer chemotherapeutic approaches. Herein, we aimed at evaluating the in vitro effect of alcoholic extracts of Artemisia annua (AMR), Rumex abyssinicus (RMA), and Catha edulis Forsk (CEF) on proliferation/viability of 1321N1 astrocytoma, MCF-7 breast cancer, THP-1 leukemia, and LNCaP, Du-145, and PC-3 prostate cancer cells and on Trypanosoma brucei cells. Proliferation of tumor cells was evaluated by WST-1 assay and viability/behaviour of T. brucei by cell counting and light microscopy. CEF was the most efficient growth inhibitor in comparison to AMR and RMA. Nevertheless, in LNCaP and THP-1 cells, all extracts significantly inhibited tumor growth at 3 μg/mL. All extracts inhibited proliferation of T. brucei cells in a concentration-dependent manner. Microscopic analysis revealed that 95% of the T. brucei cells died when exposed to 33 μg/mL CEF for 3 hrs. Similar results were obtained using 33 μg/mL AMR for 6 hrs. In case of RMA, however, higher concentrations were necessary to obtain similar effects on T. brucei. This demonstrates the antitumor efficacy of these extracts as well as their ability to dampen viability and proliferation of T. brucei, suggesting a common mechanism of action on highly proliferative cells, most probably by targeting cell metabolism. ","PeriodicalId":90189,"journal":{"name":"ISRN biochemistry","volume":"2013 ","pages":"910308"},"PeriodicalIF":0.0,"publicationDate":"2013-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4392988/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33272053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 27