ISRN biochemistry最新文献_第3页

Neuronal nicotinic receptors in sleep-related epilepsy: studies in integrative biology. 睡眠相关癫痫中的神经元烟碱受体：综合生物学研究。

ISRN biochemistry Pub Date : 2012-12-09 eCollection Date: 2012-01-01 DOI: 10.5402/2012/262941

Andrea Becchetti

{"title":"Neuronal nicotinic receptors in sleep-related epilepsy: studies in integrative biology.","authors":"Andrea Becchetti","doi":"10.5402/2012/262941","DOIUrl":"10.5402/2012/262941","url":null,"abstract":"Although Mendelian diseases are rare, when considered one by one, overall they constitute a significant social burden. Besides the medical aspects, they propose us one of the most general biological problems. Given the simplest physiological perturbation of an organism, that is, a single gene mutation, how do its effects percolate through the hierarchical biological levels to determine the pathogenesis? And how robust is the physiological system to this perturbation? To solve these problems, the study of genetic epilepsies caused by mutant ion channels presents special advantages, as it can exploit the full range of modern experimental methods. These allow to extend the functional analysis from single channels to whole brains. An instructive example is autosomal dominant nocturnal frontal lobe epilepsy (ADNFLE), which can be caused by mutations in neuronal nicotinic acetylcholine receptors. In vitro, such mutations often produce hyperfunctional receptors, at least in heterozygous condition. However, understanding how this leads to sleep-related frontal epilepsy is all but straightforward. Several available animal models are helping us to determine the effects of ADNFLE mutations on the mammalian brain. Because of the complexity of the cholinergic regulation in both developing and mature brains, several pathogenic mechanisms are possible, which also present different therapeutic implications. ","PeriodicalId":90189,"journal":{"name":"ISRN biochemistry","volume":"2012 ","pages":"262941"},"PeriodicalIF":0.0,"publicationDate":"2012-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4392997/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33179459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Feeding of Bait to Snail Lymnaea acuminata and Their Effect on Certain Enzyme in the Nervous Tissue. 给蜗牛喂食饵料及其对神经组织中某些酶的影响

ISRN biochemistry Pub Date : 2012-12-04 eCollection Date: 2012-01-01 DOI: 10.5402/2012/343047

Pradeep Kumar, V K Singh, D K Singh

{"title":"Feeding of Bait to Snail Lymnaea acuminata and Their Effect on Certain Enzyme in the Nervous Tissue.","authors":"Pradeep Kumar, V K Singh, D K Singh","doi":"10.5402/2012/343047","DOIUrl":"10.5402/2012/343047","url":null,"abstract":"Fascioliasis, a snail-borne parasitic zoonosis, has been recognized for a long time because of its major veterinary and human impact. Different Bait formulations were fed to the snail Lymnaea acuminata in clear glass aquaria having diameter of 30 cm. Snail attractant containing bait formulations was prepared from different binary combination (1 : 1 ratio) of carbohydrates (glucose, starch 10 mM) and amino acid (methionine, histidine 10 mM) in 100 ml of 2% agar solution + sublethal (20% and 60% of 24 h and 96 h LC50) doses of different molluscicides (eugenol, ferulic acid, umbelliferone, and limonene). Snails fed on bait containing sub-lethal concentration of different molluscicides and the snail attractant, causing a significant inhibition in alkaline phosphatase (ALP) and acetylcholinesterase (AChE) activity in the nervous tissue of the vector snail L. acuminata. Maximum inhibition in ALP (20% of control) and AChE (49.49% of control) activity was observed in the nervous tissue of the L. acuminata exposed to 60% of 96 h LC50 of eugenol in the bait pellets containing starch + histidine, starch + methionine, respectively. ","PeriodicalId":90189,"journal":{"name":"ISRN biochemistry","volume":"2012 ","pages":"343047"},"PeriodicalIF":0.0,"publicationDate":"2012-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4392983/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33179458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Calpain dysregulation in Alzheimer's disease. 阿尔茨海默病中的钙蛋白酶失调。

ISRN biochemistry Pub Date : 2012-10-16 eCollection Date: 2012-01-01 DOI: 10.5402/2012/728571

Adriana Ferreira

引用次数: 0

Characterization of LlaKI, a New Metal Ion-Independent Restriction Endonuclease from Lactococcus lactis KLDS4. 乳酸乳球菌KLDS4新型金属离子不依赖性限制性内切酶LlaKI的鉴定

ISRN biochemistry Pub Date : 2012-09-30 eCollection Date: 2012-01-01 DOI: 10.5402/2012/287230

Abdelkarim Belkebir, Houssine Azeddoug

{"title":"Characterization of LlaKI, a New Metal Ion-Independent Restriction Endonuclease from Lactococcus lactis KLDS4.","authors":"Abdelkarim Belkebir, Houssine Azeddoug","doi":"10.5402/2012/287230","DOIUrl":"https://doi.org/10.5402/2012/287230","url":null,"abstract":"Requirement of divalent cations for DNA cleavage is a general feature of type II restriction enzymes with the exception of few members of this group. A new type II restriction endonuclease has been partially purified from Lactococcus lactis KLDS4. The enzyme was denoted as LlaKI and showed to recognize and cleave the same site as FokI. The enzyme displayed a denatured molecular weight of 50 kDa and behaved as a dimer in solution as evidenced by the size exclusion chromatography. To investigate the role of divalent cations in DNA cleavage by LlaKI, digestion reactions were carried out at different Mg(2+), Mn(2+), and Ca(2+) concentrations. Unlike most of type II restriction endonucleases, LlaKI did not require divalent metal ions to cleave DNA and is one of the few metal-independent restriction endonucleases found in bacteria. The enzyme showed near-maximal levels of activity in 10 mM Tris-HCl pH 7.9, 50 mM NaCl, 10 mM MgCl2, and 1 mM dithiothreitol at 30°C. The presence of DNA modification was also determined and was correlated with the correspondent restriction enzyme. ","PeriodicalId":90189,"journal":{"name":"ISRN biochemistry","volume":"2012 ","pages":"287230"},"PeriodicalIF":0.0,"publicationDate":"2012-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4392985/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33179457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Constrained peptides as miniature protein structures. 作为微型蛋白质结构的受限肽。

ISRN biochemistry Pub Date : 2012-09-26 eCollection Date: 2012-01-01 DOI: 10.5402/2012/692190

Hang Yin

引用次数: 0

Withaferin a induces proteasome-dependent degradation of breast cancer susceptibility gene 1 and heat shock factor 1 proteins in breast cancer cells. Withaferin a诱导乳腺癌细胞中乳腺癌易感基因1和热休克因子1蛋白的蛋白酶体依赖性降解。

ISRN biochemistry Pub Date : 2012-09-01 eCollection Date: 2012-01-01 DOI: 10.5402/2012/707586

Xuan Zhang, Barbara Timmermann, Abbas K Samadi, Mark S Cohen

{"title":"Withaferin a induces proteasome-dependent degradation of breast cancer susceptibility gene 1 and heat shock factor 1 proteins in breast cancer cells.","authors":"Xuan Zhang, Barbara Timmermann, Abbas K Samadi, Mark S Cohen","doi":"10.5402/2012/707586","DOIUrl":"https://doi.org/10.5402/2012/707586","url":null,"abstract":"The purpose of this study was to examine the regulation of prosurvival factors heat shock factor 1 (HSF1) and breast cancer susceptibility gene 1 (BRCA1) by a natural withanolide withaferin A (WA) in triple negative breast cancer cell lines MDA-MB-231 and BT20. Western analysis was used to examine alternations in HSF1 and BRCA1 protein levels following WA treatment. A protein synthesis inhibitor cycloheximide and a proteasome inhibitor MG132 were used to investigate the mechanisms of HSF1 and BRCA1 regulation by WA. It was found that WA induced a dose-dependent decrease in HSF1 and BRCA1 protein levels. Further analysis showed that levels of HSF1 and BRCA1 proteins decreased rapidly after WA treatment, and this was attributed to WA-induced denaturation of HSF1 and BRCA1 proteins and subsequent degradation via proteasome-dependent, and protein-synthesis dependent mechanism. In summary, WA induces denaturation and proteasomal degradation of HSF1 and BRCA1 proteins. Further studies are warranted to examine the contribution of HSF1 and BRCA1 depletion to the anticancer effects of WA in breast cancer. ","PeriodicalId":90189,"journal":{"name":"ISRN biochemistry","volume":"2012 ","pages":"707586"},"PeriodicalIF":0.0,"publicationDate":"2012-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4392979/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33179460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

The effect of the combined action of roscovitine and Paclitaxel on the apoptotic and cell cycle regulatory mechanisms in colon and anaplastic thyroid cancer cells. 罗斯科维汀和紫杉醇联合作用对结肠癌和间变性甲状腺癌细胞凋亡和细胞周期调控机制的影响。

ISRN biochemistry Pub Date : 2012-08-30 eCollection Date: 2012-01-01 DOI: 10.5402/2012/826305

V V Pushkarev, O I Kovzun, V M Pushkarev, M D Tronko

{"title":"The effect of the combined action of roscovitine and Paclitaxel on the apoptotic and cell cycle regulatory mechanisms in colon and anaplastic thyroid cancer cells.","authors":"V V Pushkarev, O I Kovzun, V M Pushkarev, M D Tronko","doi":"10.5402/2012/826305","DOIUrl":"https://doi.org/10.5402/2012/826305","url":null,"abstract":"Aim. To study the significance of cyclin-dependent kinases (Cdks) in paclitaxel-dependent apoptosis in colon and undifferentiated thyroid cancer cells. Materials and Methods. Experiments were performed on undifferentiated thyroid carcinoma (KTC-2) and colon carcinoma (ARO) cell lines. Cells were treated with paclitaxel (Ptx) and inhibitor of Cdk, roscovitine. Cell survival test and Western blotting were used for characterization of the effects of paclitaxel and roscovitine on cancer cells. Results. It was shown that not c-Jun N-terminal kinase, but cyclin-dependent kinases are responsible for antiapoptotic Bcl-2 phosphorylation. Cdk inhibition enhanced the cytotoxic effects of Ptx at low drug concentrations. There was antagonism between Ptx and roscovitine at higher (25 nM) paclitaxel concentrations. Conclusion. Using of paclitaxel at low (2.5 to 5 nM) concentrations and roscovitine is a promising combination for further preclinical trials for the development of new therapeutic approaches to the treatment of colon and anaplastic thyroid cancer. ","PeriodicalId":90189,"journal":{"name":"ISRN biochemistry","volume":"2012 ","pages":"826305"},"PeriodicalIF":0.0,"publicationDate":"2012-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4392990/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33298672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

The essence of ATP coupling. ATP耦合的本质。

ISRN biochemistry Pub Date : 2012-05-09 eCollection Date: 2012-01-01 DOI: 10.5402/2012/827604

Nikolai Bazhin

引用次数: 3