ISRN biochemistry最新文献_第2页

Human sprouty1 suppresses urokinase receptor-stimulated cell migration and invasion. 人sprouty1抑制尿激酶受体刺激的细胞迁移和侵袭。

ISRN biochemistry Pub Date : 2013-09-12 eCollection Date: 2013-01-01 DOI: 10.1155/2013/598251

Ahmed H Mekkawy, David L Morris

{"title":"Human sprouty1 suppresses urokinase receptor-stimulated cell migration and invasion.","authors":"Ahmed H Mekkawy, David L Morris","doi":"10.1155/2013/598251","DOIUrl":"https://doi.org/10.1155/2013/598251","url":null,"abstract":"The urokinase-type plasminogen activator receptor (uPAR) has been implicated in several processes in tumor progression including cell migration and invasion in addition to initiation of signal transduction. Since uPAR lacks a transmembrane domain, it uses the interaction with other proteins to modulate intracellular signal transduction. We have previously identified hSpry1 as a partner protein of uPAR, suggesting a physiological role for hSpry1 in the regulation of uPAR signal transduction. In this study, hSpry1 was found to colocalize with uPAR upon stimulation with epidermal growth factor (EGF), urokinase (uPA), or its amino terminal fragment (uPA-ATF), implicating a physiological role of hSpry1 in regulation of uPAR signalling pathway. Moreover, hSpry1 was able to inhibit uPAR-stimulated cell migration in HEK293/uPAR, breast carcinoma, and colorectal carcinoma cells. In addition, hSpry1 was found to inhibit uPAR-stimulated cell invasion in breast carcinoma and osteosarcoma cell lines. Increasing our understanding of how hSpry1 negatively regulates uPAR-stimulated cellular functions may determine a distinctive role for hSpry1 in tumour suppression. ","PeriodicalId":90189,"journal":{"name":"ISRN biochemistry","volume":"2013 ","pages":"598251"},"PeriodicalIF":0.0,"publicationDate":"2013-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4393002/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33272051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

A sequence-specific nicking endonuclease from streptomyces: purification, physical and catalytic properties. 链霉菌的序列特异性缺口内切酶:纯化、物理和催化性质。

ISRN biochemistry Pub Date : 2013-08-21 eCollection Date: 2013-01-01 DOI: 10.1155/2013/287158

Peechapack Somyoonsap, Vichein Kitpreechavanich, Somchai Pornbanlualap

{"title":"A sequence-specific nicking endonuclease from streptomyces: purification, physical and catalytic properties.","authors":"Peechapack Somyoonsap, Vichein Kitpreechavanich, Somchai Pornbanlualap","doi":"10.1155/2013/287158","DOIUrl":"https://doi.org/10.1155/2013/287158","url":null,"abstract":"A sequence-specific nicking endonuclease from Streptomyces designated as DC13 was purified to near homogeneity. Starting with 30 grams of wet cells, the enzyme was purified by ammonium sulfate fractionation, DEAE cellulose, and phenyl-Sepharose chromatography. The purified protein had a specific activity 1000 units/mg and migrated on SDS-PAGE gel with an estimated molecular weight of 71 kDa. Determination of subunit composition by gel filtration chromatography indicated that the native enzyme is a monomer. When incubated with different DNA substrates including pBluescript II KS, pUC118, pET-15b, and pET-26b, the enzyme converted these supercoiled plasmids to a mixture of open circular and linear DNA products, with the open circular DNA as the major cleavage product. Analysis of the kinetic of DNA cleavage showed that the enzyme appeared to cleave super-coiled plasmid in two distinct steps: a rapid cleavage of super-coiled plasmid to an open circular DNA followed a much slower step to linear DNA. The DNA cleavage reaction of the enzyme required Mg(2+) as a cofactor. Based on the monomeric nature of the enzyme, the kinetics of DNA cleavage exhibited by the enzyme, and cofactor requirement, it is suggested here that the purified enzyme is a sequence-specific nicking endonuclease that is similar to type IIS restriction endonuclease. ","PeriodicalId":90189,"journal":{"name":"ISRN biochemistry","volume":"2013 ","pages":"287158"},"PeriodicalIF":0.0,"publicationDate":"2013-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/287158","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33272048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biochemical Studies on Methylglyoxal-Mediated Glycated Histones: Implications for Presence of Serum Antibodies against the Glycated Histones in Patients with Type 1 Diabetes Mellitus. 对甲基乙二醛介导的糖化组蛋白的生化研究：1 型糖尿病患者血清中存在针对糖化组蛋白的抗体的意义。

ISRN biochemistry Pub Date : 2013-08-07 eCollection Date: 2013-01-01 DOI: 10.1155/2013/198065

Nadeem A Ansari, Debabrata Dash

{"title":"Biochemical Studies on Methylglyoxal-Mediated Glycated Histones: Implications for Presence of Serum Antibodies against the Glycated Histones in Patients with Type 1 Diabetes Mellitus.","authors":"Nadeem A Ansari, Debabrata Dash","doi":"10.1155/2013/198065","DOIUrl":"10.1155/2013/198065","url":null,"abstract":"Reactive carbonyl species (RCS) mainly reacts with lysine and arginine residues of proteins to form advanced glycation end products (AGEs). Histone was glycoxidated with glyoxal and methylglyoxal. It was characterized by polyacrylamide gel electrophoresis and quenching studies involving penicillamine and aminoguanidine as carbonyl scavengers. Further characterization of histone modified with methylglyoxal was done by UV, fluorescence, and IR spectrophotometry. Spectral analysis of the protein clearly demonstrates structural perturbation in the histone by methylglyoxal. Methylglyoxal-induces cross-linking in the protein leading to aggregation. Role of methylglyoxal mediated glycoxidation of histone in type 1 diabetes was also undertaken. Antibodies were detected against glycoxidated histone in sera of type 1 diabetes patients by solid-phase enzyme immunoassay. The findings indicate that as a result of structural perturbation in histone by methylglyoxal, the modified histone may be involved in production of serum antibodies in the diabetes patients. ","PeriodicalId":90189,"journal":{"name":"ISRN biochemistry","volume":"2013 ","pages":"198065"},"PeriodicalIF":0.0,"publicationDate":"2013-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4392999/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33272047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cellular and Biochemical Mechanisms of the Retroviral Restriction Factor SAMHD1. 逆转录病毒限制因子SAMHD1的细胞和生化机制。

ISRN biochemistry Pub Date : 2013-07-07 DOI: 10.1155/2013/728392

Li Wu

引用次数: 20

Nanoparticles for brain drug delivery. 用于脑部给药的纳米颗粒。

ISRN biochemistry Pub Date : 2013-05-21 eCollection Date: 2013-01-01 DOI: 10.1155/2013/238428

Massimo Masserini

{"title":"Nanoparticles for brain drug delivery.","authors":"Massimo Masserini","doi":"10.1155/2013/238428","DOIUrl":"10.1155/2013/238428","url":null,"abstract":"The central nervous system, one of the most delicate microenvironments of the body, is protected by the blood-brain barrier (BBB) regulating its homeostasis. BBB is a highly complex structure that tightly regulates the movement of ions of a limited number of small molecules and of an even more restricted number of macromolecules from the blood to the brain, protecting it from injuries and diseases. However, the BBB also significantly precludes the delivery of drugs to the brain, thus, preventing the therapy of a number of neurological disorders. As a consequence, several strategies are currently being sought after to enhance the delivery of drugs across the BBB. Within this review, the recently born strategy of brain drug delivery based on the use of nanoparticles, multifunctional drug delivery systems with size in the order of one-billionth of meters, is described. The review also includes a brief description of the structural and physiological features of the barrier and of the most utilized nanoparticles for medical use. Finally, the potential neurotoxicity of nanoparticles is discussed, and future technological approaches are described. The strong efforts to allow the translation from preclinical to concrete clinical applications are worth the economic investments. ","PeriodicalId":90189,"journal":{"name":"ISRN biochemistry","volume":"2013 ","pages":"238428"},"PeriodicalIF":0.0,"publicationDate":"2013-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4392984/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33272049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cav1.2, cell proliferation, and new target in atherosclerosis. Cav1.2，细胞增殖，动脉粥样硬化的新靶点。

ISRN biochemistry Pub Date : 2013-05-12 eCollection Date: 2013-01-01 DOI: 10.1155/2013/463527

Nikolai M Soldatov

引用次数: 6

Metabolic Regulation of a Bacterial Cell System with Emphasis on Escherichia coli Metabolism. 细菌细胞系统的代谢调控，重点是大肠杆菌的代谢。

ISRN biochemistry Pub Date : 2013-02-18 eCollection Date: 2013-01-01 DOI: 10.1155/2013/645983

Kazuyuki Shimizu

引用次数: 0

Role of Elicitors in Inducing Resistance in Plants against Pathogen Infection: A Review. 诱导剂在诱导植物抵抗病原体感染中的作用：综述。

ISRN biochemistry Pub Date : 2013-01-28 eCollection Date: 2013-01-01 DOI: 10.1155/2013/762412

Meenakshi Thakur, Baldev Singh Sohal

{"title":"Role of Elicitors in Inducing Resistance in Plants against Pathogen Infection: A Review.","authors":"Meenakshi Thakur, Baldev Singh Sohal","doi":"10.1155/2013/762412","DOIUrl":"10.1155/2013/762412","url":null,"abstract":"Disease control is largely based on the use of fungicides, bactericides, and insecticides-chemical compounds toxic to plant invaders, causative agents, or vectors of plant diseases. However, the hazardous effect of these chemicals or their degradation products on the environment and human health strongly necessitates the search for new, harmless means of disease control. There must be some natural phenomenon of induced resistance to protect plants from disease. Elicitors are compounds, which activate chemical defense in plants. Various biosynthetic pathways are activated in treated plants depending on the compound used. Commonly tested chemical elicitors are salicylic acid, methyl salicylate, benzothiadiazole, benzoic acid, chitosan, and so forth which affect production of phenolic compounds and activation of various defense-related enzymes in plants. Their introduction into agricultural practice could minimize the scope of chemical control, thus contributing to the development of sustainable agriculture. This paper chiefly highlights the uses of elicitors aiming to draw sufficient attention of researchers to the frontier research needed in this context. ","PeriodicalId":90189,"journal":{"name":"ISRN biochemistry","volume":"2013 ","pages":"762412"},"PeriodicalIF":0.0,"publicationDate":"2013-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4393000/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33298674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Locus-specific biochemical epigenetics/chromatin biochemistry by insertional chromatin immunoprecipitation. 插入染色质免疫沉淀的基因座特异性生化表观遗传学/染色质生化。

ISRN biochemistry Pub Date : 2013-01-10 eCollection Date: 2013-01-01 DOI: 10.1155/2013/913273

Toshitsugu Fujita, Hodaka Fujii

引用次数: 20

Ganglioside biochemistry. 神经节苷脂生物化学。

ISRN biochemistry Pub Date : 2012-12-19 eCollection Date: 2012-01-01 DOI: 10.5402/2012/506160

Thomas Kolter

引用次数: 108