International journal of biotechnology for wellness industries最新文献

{"title":"Paraconiothyrium sp. P83F4/1: antioxidant and antiproliferative activities of endophytic fungus associated with Rheedia brasiliensis plant","authors":"Patrícia Lunardelli Negreiros de Carvalho, Priscilla Oliveira Amaral, Ana Lúcia Tasca Gois Ruiz, S. M. Alencar, L. Pfenning, J. Carvalho, P. Rosalen, M. Ikegaki","doi":"10.6000/1927-3037/2012.01.03.03","DOIUrl":"https://doi.org/10.6000/1927-3037/2012.01.03.03","url":null,"abstract":"The endophyte Paraconiothyrium sp. P83F4/1 was isolated from leaves of the Rheedia brasiliensis medicinal plant from a bioprospecting study. This is the first report of isolation of Paraconiothyrium in leaves of R. brasiliensis and the fermentation product obtained with this fungus in ethyl acetate was evaluated for their potential bioactive through antioxidant assay by DPPH (radical 1,1-diphenyl-2-picrylhydrazyl) method and antiproliferative assays in human tumoral cells using the sulphorhodamine B (SRB) technique for evaluating the cellular growth. The antioxidant assay, tested at 90 mg/mL, the fungal extract showed scavenging capacity of 58.92 %, being significant (p<0.05) compared the standard commercial butyl hydroxy toluene: 39.52 %. In the antiproliferative assay, the extract was selective on human keratinocyte cells (HaCat) with GI 50 0.95 mg/mL, whose mean log GI 50 = - 0.02, according to National Cancer Institute (NCI-USA) criteria, it makes a powerful extract. Cells HaCat are involved directly in diseases such as psoriasis. These results indicate the antioxidant and antiproliferative potentials of the crude extract of fermentation of the endophytic fungus Paraconiothyrium sp. P83F4/1 isolated from the leaves of R. brasiliensis.","PeriodicalId":90181,"journal":{"name":"International journal of biotechnology for wellness industries","volume":"1 1","pages":"172-176"},"PeriodicalIF":0.0,"publicationDate":"2012-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71249818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Photo-Protective Activity of Crataegus monogyna Extract Proved by Complementary Mechanisms of Skin Inflammation, Damage of Extracellular Matrix, Cellular Survival and Proliferation Disorders","authors":"B. Dumitriu, L. Olariu, D. Ene, L. Zglimbea, N. Roșoiu","doi":"10.6000/1927-3037/2012.01.03.04","DOIUrl":"https://doi.org/10.6000/1927-3037/2012.01.03.04","url":null,"abstract":"The skin photo-ageing and photo-carcinogenesis are based on cellular mechanisms triggered by UV-radiation, starting with oxidative stress and inflammation, activation of matrix-metalloproteinase (MMP) that increase the structural protein degradation, ending with DNA damage and apoptosis induction. In order to claim the photo-protective activity of an ingredient it’s important to explore the main pathways involved in this complex process, highlighting its target action. Our studies are based on a cellular screening of UV-A and UV-B irradiated keratinocytes (HaCat cell line), focusing on the apoptosis induction, cell cycle sequentiation, metalloproteinase’s expression, pro-inflammatory cytokines release and IL1α as sensitization molecule, as well as the antioxidant capacity of the extract, and acting on catalase and superoxide-dismutase activity. The investigation techniques are flow cytometry (cellular parameters fluorescent staining and beads-based assay for soluble protein detection), zymography and enzymatic activity tests. The Crataegus monogyna extract, with a rich content of pro-antocians, flavones and polyphenolcarboxylic acids, show a UV-A cellular protection and dose dependent inhibition of inflammatory cytokines and MMP2 and 9 after both UV-A and UV-B irradiation. All these effects are sustained by a cumulative antioxidant capacity at enzymatic and intracellular oxygen radical level. These findings recommend the phyto-complex for the prevention or therapy of UV-photo damages.","PeriodicalId":90181,"journal":{"name":"International journal of biotechnology for wellness industries","volume":"1 1","pages":"177-188"},"PeriodicalIF":0.0,"publicationDate":"2012-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71249842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Probiotics for everyone! The novel immunobiotic lactobacillus rhamnosus CRL1505 and the beginning of social probiotic programs in Argentina","authors":"J. Villena, S. Salva, M. Núñez, J. Corzo, René Tolaba, Julio Faedda, G. Font, S. Alvarez","doi":"10.6000/1927-3037/2012.01.03.05","DOIUrl":"https://doi.org/10.6000/1927-3037/2012.01.03.05","url":null,"abstract":"Lactobacillus rhamnosus CRL1505 (Lr1505) stimulates immune responses in the gut and in the respiratory tract and improves resistance against Salmonella typhimurium and Streptococcus pneumoniae infections in immunocompetent and immunocompromised mice. Considering that respiratory infectious diseases continue to be a major cause of death among preschool children in developing countries, the aim of the present study was to evaluate the effect of Lr1505 on the health of children. A randomized-controlled double-blind clinical trial in 298 healthy children (2-5 years old), attending daycare centers was performed. Yogurt containing Lr1505 was administered to children for 6 months (five times a week). Results were statistically compared with those of children from the same community that received a placebo yogurt (without probiotic). Administration of Lr1505 to young children reduced the incidence of infections: 66% of children in the placebo group presented symptoms of infection while only 34% of cases were detected in the Lr1505 group. Significant differences ( P <0.05) were detected in the incidence of intestinal infections, upper respiratory tract infections and angina when placebo and Lr1505 groups were compared. Children fed Lr1505 experienced fewer fevers and needed fewer antibiotics than those receiving the placebo. The protective effect of Lr1505 was associated with increased levels of mucosal IgA antibodies. Lr1505 is a promising resource for the development of prevention strategies against mucosal infections that could be effective tools for medical application. This new probiotic strain has been included into official Nutritional Programs in Argentina and it is given to more than 200 thousand children. This project has encouraged local milk production, thanks to the constant demand of probiotic yogurt containing L. rhamnosus CRL1505 by provincial governments, while incorporating innovation to small and medium enterprises.","PeriodicalId":90181,"journal":{"name":"International journal of biotechnology for wellness industries","volume":"1 1","pages":"189-198"},"PeriodicalIF":0.0,"publicationDate":"2012-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71249879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rosemary and Sage Outperformed Six other Culinary Herbs in Antioxidant and Antibacterial PropertiesRosemary and Sage Outperformed Six other Culinary Herbs in Antioxidant and Antibacterial Properties","authors":"E. Chan, L. Kong, K. Y. Yee, W. Chua, T. Loo","doi":"10.6000/1927-3037/2012.01.02.06","DOIUrl":"https://doi.org/10.6000/1927-3037/2012.01.02.06","url":null,"abstract":"The antioxidant and antibacterial properties of Labiatae culinary herbs are well documented but the effects of different drying methods are poorly studied. In this study, the antioxidant and antibacterial properties of fresh and oven-dried herbs of oregano, marjoram, rosemary, sage, basil, thyme, peppermint and spearmint were compared with available commercial herbs. Antioxidant properties of total phenolic content, total flavonoid content, caffeoylquinic acid content, free radical scavenging activity, ferric reducing power and ferrous ion chelating ability were assessed using the Folin-Ciocalteu, aluminium chloride, molybdate, DPPH radical scavenging, potassium ferricyanide and ferrozine assays, respectively. Antibacterial properties were assessed using the disc diffusion assay based on mean diameter of inhibitory zone and minimum inhibitory dose. The two drying treatments were oven drying at 50 o C (OD) and microwave pre-treatment followed by oven drying at 50 o C (MOD). Fresh rosemary and dried oregano had the strongest antioxidant properties. For most herbs, oven drying resulted in loss of  antioxidant values compared to fresh herbs with the exception of oregano. Values of OD and MOD oregano, spearmint, thyme, peppermint and basil were higher than commercial samples, while those of rosemary were lower. Of the six commercial herbs, rosemary had the highest values, followed by oregano, spearmint, thyme, peppermint and basil. Rosemary, sage, peppermint and spearmint inhibited the growth of all three Gram-positive bacteria of Bacillus cereus , Micrococcus luteus and Staphylococcus aureus . Rosemary and sage had stronger antibacterial properties than green and black teas of Camellia sinensis . When used in combination, rosemary and sage can have enhanced antioxidant and antibacterial effects, which are desirable in developing nutraceutical products, and in controlling rancidity and bacterial growth in food.","PeriodicalId":90181,"journal":{"name":"International journal of biotechnology for wellness industries","volume":"1 1","pages":"142-151"},"PeriodicalIF":0.0,"publicationDate":"2012-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71250167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the Effects of Several Parameters on the Growth of Chlorella vulgaris Using Taguchi's Experimental Approach","authors":"R. Barghbani, K. Rezaei, A. Javanshir","doi":"10.6000/1927-3037/2012.01.02.04","DOIUrl":"https://doi.org/10.6000/1927-3037/2012.01.02.04","url":null,"abstract":"Algae are part of photosynthetic organisms that play an important role in the aquatics nutrition. Like plants, algae need water, light and CO 2 to grow. Using Taguchi’s experimental approach (5 factors in four levels with 16 runs), effects of several parameters (NaCl, sodium bicarbonate and iron concentrations as well as light and temperature) on the growth of Chlorella vulgaris was studied. Increasing the concentrations of NaCl and sodium bicarbonate resulted in corresponding decreases in the growth of C. vulgaris . Media with 30.0g l -1 NaCl did not indicate any algal growth. Also, with 9.0 g l -1 sodium bicarbonate, biomass production was decreased. Chlorella vulgaris showed different growing behaviors at the various concentrations of iron (Fe +2 ) and at the different temperatures of this study . Maximum biomass production (approximately 3.56 g dry matter) was obtained at the 0.0 g l -1 sodium bicarbonate, 10.0g l -1 NaCl, 18.0 µmol l -1 iron and at 30±2 °C. Yellow and blue lights increased the algal growth. Analysis of variance showed that salinity (i.e., the NaCl concentration) had the highest impact on the biomass production.","PeriodicalId":90181,"journal":{"name":"International journal of biotechnology for wellness industries","volume":"12 1","pages":"128-133"},"PeriodicalIF":0.0,"publicationDate":"2012-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71250211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dilute Solution Viscometry Studies on a Therapeutic Mixture of Non-digestible Carbohydrates","authors":"S. Harding, F. Almutairi, G. Adams, G. Morris, C. J. Lawson, R. Gahler, S. Wood, Clifton Boulevard, E. Gate","doi":"10.6000/1927-3037/2012.01.02.01","DOIUrl":"https://doi.org/10.6000/1927-3037/2012.01.02.01","url":null,"abstract":"Recent work has shown the beneficial effects of a proprietary mixture of three non-digestible carbohydrates: konjac glucomannan, xanthan and alginate and these effects have been linked with a synergistic interaction observable with analytical ultracentrifugation, rheological and NMR measurements. These observations have been supported by fundamental dilute solution viscosity studies. Preparations of konjac glucomannan, xanthan and alginate have been checked with regards their molecular integrity (molar mass distribution) using a newly established method based on the analytical ultracentrifuge. The intrinsic viscosity behaviour for each of the individual polysaccharides were estimated at low ionic strength I (10 -3 M) and found to be (2090±120) ml/g, (4430±340) ml/g and (3460±330) ml/g for konjac glucomannan, xanthan and alginate respectively and at (10 -1 M) (2350±200) ml/g, (3370±310) ml/g and (1210±50) ml/g respectively. The intrinsic viscosity (η) was then determined for a proprietary mixture of the three (known as \"PGX®\") at both ionic strengths and compared with the predicted values for a non-interacting mixture. In I=10 -3 M solvent a significant difference was observed (3090+250) ml/g compared with the predicted value (2350+300) ml/g, although at higher ionic strength the interaction appears to have gone: (η) = (1990+250) ml/g compared with the predicted value of (2180+300) ml/g. This appears to reinforce the earlier observations that in PGX® there is a synergistic interaction which is ionic strength sensitive.","PeriodicalId":90181,"journal":{"name":"International journal of biotechnology for wellness industries","volume":"1 1","pages":"107-114"},"PeriodicalIF":0.0,"publicationDate":"2012-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71249983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial Activities of Extracts and Flavonoid Glycosides of Corn Silk (Zea mays L)","authors":"F. Nessa, Z. Ismail, Nornisah Mohamed","doi":"10.6000/1927-3037/2012.01.02.02","DOIUrl":"https://doi.org/10.6000/1927-3037/2012.01.02.02","url":null,"abstract":"Corn silk refers to the stigmas of Zea mays L. ( Gramineae ) from the female flowers of maize. It is medicinally used in a number of diseases and contained a number of flavonoids. Screening of plants against pathogenic bacteria is an important step to validate its medicinal properties. Therefore, the aim of this study was to screen the antimicrobial activities of different solvent extracts, flavonoids of corn silk and compare the activities with standard antibiotic gentamycin. The pet-ether (PECS), chloroform (CECS) and methanol (MECS) extracts (25 mg/mL) of corn silk were tested for their antimicrobial activity. Twelve pathogenic bacteria such as Bacillus cereus, Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Enterobacter aerogenase, Salmonella typhi, Salmonella paratyphi, Escherichia coli, Shigella sonneii, Shigella flexneri, Proteus vulgaris, Proteus mirabilis and one fungus Candida albicans were used to screen the extracts. Gentamycin  (50 mg/mL) was used as reference antibiotic. Two isolated flavonoid glycosides (2.0 mg/mL) of corn silk were tested for their antimicrobial activity. The microbial growth inhibitory potential was determined by using the agar hole-plate diffusion method. PECS, MECS and flavonoids were sensitive against eleven bacteria out of twelve bacteria. CECS was sensitive only against five bacteria.  No extracts and flavonoids were sensitive against Escherichia coli and Candid albicans . The results were compared with gentamycin, which was sensitive against all the bacteria tested. Extracts and flavonoids showed significantly (p<0.05) higher sensitivity against a number of bacteria than gentamycin.","PeriodicalId":90181,"journal":{"name":"International journal of biotechnology for wellness industries","volume":"1 1","pages":"115-120"},"PeriodicalIF":0.0,"publicationDate":"2012-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71250040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Cuspidaria pulchra and its Isolated Compounds Against Schistosoma mansoni Adult Worms","authors":"T. A. Alvarenga, Tais R.F.O. Bêdo, C. G. Braguine, Uo Gonçalves, L. Magalhães, V. Rodrigues, V. Gimenez, M. Groppo, M. Silva, W. Cunha, A. Januário, P. Pauletti","doi":"10.6000/1927-3037/2012.01.02.03","DOIUrl":"https://doi.org/10.6000/1927-3037/2012.01.02.03","url":null,"abstract":"The present study has investigated the chemical composition of the bioactive EtOAc fraction of Cuspidaria pulchra aerial parts, as well as its schistosomicidal activities against Schistosoma mansoni adult worms in vitro . To this end, the crude ethanol extract obtained from the aerial parts of C. pulchra (Bignoniaceae) was partitioned with n -hexane, EtOAc, and n -BuOH. The EtOAc fraction was purified by preparative HPLC, which afforded 3,4-dihydroxybenzaldehyde ( 1 ), p- coumaric acid ( 2 ), p -hydroxybenzoic acid ( 3 ), ursolic acid ( 4 ), and oleanolic acid ( 5 ). The bioassay results indicated that the crude ethanol extract and the EtOAc fraction at 100 µg/mL killed the adult schistosomes in vitro . Compounds 1 and 3 at 100 µm were only able to separate coupled S. mansoni adult worms.","PeriodicalId":90181,"journal":{"name":"International journal of biotechnology for wellness industries","volume":"1 1","pages":"121-127"},"PeriodicalIF":0.0,"publicationDate":"2012-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71250196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preliminary Study of Anticoccidial Activity of Medium Chain Fatty Acids (MCFA) and their Corresponding Monoglycerides on Broiler Chicken Coccidiosis","authors":"G. H. Tan, K. Long","doi":"10.6000/1927-3037/2012.01.02.05","DOIUrl":"https://doi.org/10.6000/1927-3037/2012.01.02.05","url":null,"abstract":"Abstract: The effect of anticoccidial from oil containing medium chain fatty acids and their corresponding monoglycerides, which known as enhanced virgin coconut oil (EVCO) was investigated. A total of 100 broiler chicks were assigned to four treatment groups. Group A, B and C were infected with 8 species of live coccidia. Group A was then treated with 30% EVCO; Group B with antibiotic (Coccisul-Q) and no treatment for Group C. Group D as control. The birds infected with live coccidia (Group A, B and C) had significantly higher in the oocyst count and showed blood in droppings appeared after 7-day post-infection (DPI), except Group D. In Group A2 at 11-day post infection/4-day post treatment (DPI 11/ DPT 4), the number of oocyst count was dropped from 132,400 o/g to 600 o/g; and in Group B2 is about 75,600 o/g to 0 o/g. However, there was no significant reducing in Group C2. A significant deduction of oocysts level in faecal and cecal dramatically after 4 days post-treatment in group treated with EVCO. However, from the observation, the performance of EVCO was not as good as in the antibiotic treated group. Nevertheless, the EVCO is a plant-based compounds, it is good and safe to be used as alternative way to control coccidiosis in poultry industries. Therefore, for EVCO consumption, the treatment must be provided weekly for long lasting protection from coccidiosis.","PeriodicalId":90181,"journal":{"name":"International journal of biotechnology for wellness industries","volume":"1 1","pages":"134-141"},"PeriodicalIF":0.0,"publicationDate":"2012-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71250160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Initial Sugar Concentration on the Production of L (+) Lactic Acid by Simultaneous Enzymatic Hydrolysis and Fermentation of an Agro-Industrial Waste Product of Pineapple (Ananas comosus) Using Lactobacillus casei Subspecies rhamnosus","authors":"Carla Araya-Cloutier, Carolina Rojas-Garbanzo, Carmela Velázquez-Carrillo","doi":"10.6000/1927-3037.2012.01.01.07","DOIUrl":"https://doi.org/10.6000/1927-3037.2012.01.01.07","url":null,"abstract":"Production of lactic acid by fermentation process has been studied from glucose solutions and other sources because of many important reasons: biotechnological production is cheaper than chemical synthesis; production of biodegradable materials from L (+) lactic acid and, the use of nutrient-rich agro-industrial wastes as raw material, which helps to reduce the environmental impact. The goal of this study was to evaluate the effect of sugar concentration of a pineapple liquid waste as the carbon source on the capacity of Lactobacillus casei subspecies rhamnosus to produce lactic acid by simultaneous enzymatic hydrolysis and fermentation. Three different pineapple waste concentrations were evaluated (60, 80 and 100% v/v) from a pineapple juice with 11.3% (m/v) of sugars (sucrose, fructose and glucose). L. casei was able to consume all sugars present within the levels tested, and converted all into lactic acid, showing efficient yields of 0.91 g lactic acid/g sugars. Final lactic acid concentration increased significantly (p<0.05) with the increase of pineapple waste percentage. Maximum lactic acid concentration (102g/L) was achieved with 100% pineapple waste medium. The highest total productivity (4.0g/h) and maximum productivity (4.48 g/L*h) were obtained with 60% pineapple waste medium and it decreased significantly (p<0.05) when 100% was used. Fermentation time increased with the increment of sugars, but it increased considerably with the medium composed of 100% of pineapple waste in comparison with the other two mediums. Pineapple waste represents a good alternative as a cheap carbon source for bacteria growth and production of L (+) lactic acid.","PeriodicalId":90181,"journal":{"name":"International journal of biotechnology for wellness industries","volume":"1 1","pages":"91-100"},"PeriodicalIF":0.0,"publicationDate":"2012-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71248405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}