International journal of biotechnology for wellness industries最新文献_第7页

Facile and novel strategy for methods of extraction of biofuel grade lipids from microalgae - an experimental report. 从微藻中提取生物燃料级脂质的简单而新颖的方法——实验报告。

International journal of biotechnology for wellness industries Pub Date : 2014-01-06 DOI: 10.6000/1927-3037.2014.03.04.2

E. Baldev, D. MubarakAli, M. Dhivya, M. Kanimozhi, Thajuddin Shakena-Fathima, N. S. Alharbi, C. Arunachalam, S. Alharbi, N. Thajuddin

{"title":"Facile and novel strategy for methods of extraction of biofuel grade lipids from microalgae - an experimental report.","authors":"E. Baldev, D. MubarakAli, M. Dhivya, M. Kanimozhi, Thajuddin Shakena-Fathima, N. S. Alharbi, C. Arunachalam, S. Alharbi, N. Thajuddin","doi":"10.6000/1927-3037.2014.03.04.2","DOIUrl":"https://doi.org/10.6000/1927-3037.2014.03.04.2","url":null,"abstract":"The structural features of microalgal cell make it too difficult to extract the total lipid content of the cell as such. Thus, the cell disruption before lipid extraction becomes mandatory and has to be cost-effective. In the present study various methods and combination of few methods were adopted for effective extraction in order to choose the most effective cell disruption method for the complete extraction of lipids from a selected indigenous freshwater isolate, Scenedesmus sp. NTEB03. Interestingly, we found that grinding and bead-beating method showed two fold increased lipid productivity (23.2%) than the other methods tested. Biomass and lipid productivity of Scenedesmus sp., was found to be 0.0418 g L -1 d -1 and 4.3 mg L -1 d -1 respectively. Fatty acid profiles revealed that oleic (C18:1) and linoleic acid (C18:2) content being higher in the lipids, which are most appropriate for the biodiesel production. A novel strategy for most effective, simple method for cell disruption in Scenedesmus sp., was grinding/bead-beating, which is the most suitable method for complete extraction of biofuel grade lipids.","PeriodicalId":90181,"journal":{"name":"International journal of biotechnology for wellness industries","volume":"3 1","pages":"121-127"},"PeriodicalIF":0.0,"publicationDate":"2014-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71248967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

World's agricultural production and trade - food security at stake? 世界农业生产和贸易——粮食安全岌岌可危?

International journal of biotechnology for wellness industries Pub Date : 2013-12-31 DOI: 10.6000/1927-3037.2013.02.04.5

MiklÃ³s Somai

引用次数: 2

Highly Efficient Extraction of Aromatic Compounds from Houttuyniacordata thumb by Cryogenic Grinding Techniques 低温研磨高效提取鱼腥草中芳香族化合物的研究

International journal of biotechnology for wellness industries Pub Date : 2013-12-31 DOI: 10.6000/1927-3037.2013.02.04.1

Qing-Song Xue

{"title":"Highly Efficient Extraction of Aromatic Compounds from Houttuyniacordata thumb by Cryogenic Grinding Techniques","authors":"Qing-Song Xue","doi":"10.6000/1927-3037.2013.02.04.1","DOIUrl":"https://doi.org/10.6000/1927-3037.2013.02.04.1","url":null,"abstract":"The current study was focused on the extraction of essential oil from Houttuyniacordata thunb (HCT) by using different solvents (ethyl acetate, ethanol, and n- hexane) using cryogenic grinding techniques (CGT). The influence of the storage time of the HCT extract on the volatile components was investigated. This paper also discussed and interpreted the mass spectral fragmentation patterns for the three new compounds (undenoyl acetaldehyde, dodenoyl acetaldehyde, and decyl-imine acetaldehyde). The results showed that the extractability of essential oil obtained by CGT using ethyl acetate as an extractant was as high as 1.14% and the content of decanoyl acetaldehyde in the essential oil was as much as 55.96%, much higher than the previously reported values. It was suggested that the oxidation and decomposition of those unstable components were effectively reduced when the essential oil was extracted by CGT. The content of decanoyl acetaldehyde in the HCT extractant remained constantly when using ethyl acetate as a solvent in the process of storage, while using ethanol as a solvent, the content of decanoyl acetaldehyde decreased rapidly as the storage time increased. The author proposed that the ethanol reacted with decanoyl acetic acid and formed decanoyl acetic ether, which accelerated the decomposition of the decanoyl acetaldehyde.","PeriodicalId":90181,"journal":{"name":"International journal of biotechnology for wellness industries","volume":"2 1","pages":"145-152"},"PeriodicalIF":0.0,"publicationDate":"2013-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71249136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antiulcerogenic, Anti-Secretory and Cytoprotective Effects of Piper Cubeba (L.) on Experimental Ulcer Models in Rat 胡椒对实验性大鼠溃疡模型的抗溃疡、抗分泌及细胞保护作用

International journal of biotechnology for wellness industries Pub Date : 2013-12-31 DOI: 10.6000/1927-3037.2013.02.04.4

M. Alsaid, I. Al-Mofleh, Mohammad Raish, Mohammed Al-Sobaihani, M. Al-yahya, S. Rafatullah

{"title":"Antiulcerogenic, Anti-Secretory and Cytoprotective Effects of Piper Cubeba (L.) on Experimental Ulcer Models in Rat","authors":"M. Alsaid, I. Al-Mofleh, Mohammad Raish, Mohammed Al-Sobaihani, M. Al-yahya, S. Rafatullah","doi":"10.6000/1927-3037.2013.02.04.4","DOIUrl":"https://doi.org/10.6000/1927-3037.2013.02.04.4","url":null,"abstract":"This paper evaluated anti-gastric ulcer and anti-secretory effects of a popular spice Piper cubeba L, (Family: Piperaceae) in rats. The gastric ulcer protective potential of an aqueous suspension of Piper cubeba (PCS) was evaluated against different acute gastric ulcer models in rats induced by pyloric ligation (Shay), hypothermic restraint stress, indomethacin and by necrotizing agents (80% ethanol, 0.2 M NaOH and 25% NaCl) induced gastric mucosal injury. Piper cubeba aqueous suspension (PCS) at the doses 250 and 500 mg/kg body weight administered orally (intraperitoneally in Shay rat model) showed a dose-dependent ulcer protective effects in all the above models. Besides, the PCS offered protection against ethanol-induced depletion of gastric wall mucus (GWM); replenished the reduced non-protein sulfhydryls (NP‑SH) concentration and significantly replenished malondialdehyde (MDA) contents in the gastric tissue. Ethanol induced histopathological lesions of the stomach wall characterized by mucosal hemorrhages and edema was reversed by Piper cubeba aqueous suspension treatment. Pretreatment of rats with Piper cubeba provided significant protection of gastric mucosa through its antioxidant capacity and/or by attenuating the offensive and by enhancing the defensive factor.","PeriodicalId":90181,"journal":{"name":"International journal of biotechnology for wellness industries","volume":"2 1","pages":"173-181"},"PeriodicalIF":0.0,"publicationDate":"2013-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71248789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Generation of New Hybridoma UTM-Ha Secreting Monoclonal Antibody Specific to Acanthamoeba species Isolated from Corneal Infection 分泌角膜感染棘阿米巴单克隆抗体的新杂交瘤UTM-Ha的产生

International journal of biotechnology for wellness industries Pub Date : 2013-12-31 DOI: 10.6000/1927-3037.2013.02.04.3

H. Rasheed, N. M. Amin, F. Majid

{"title":"Generation of New Hybridoma UTM-Ha Secreting Monoclonal Antibody Specific to Acanthamoeba species Isolated from Corneal Infection","authors":"H. Rasheed, N. M. Amin, F. Majid","doi":"10.6000/1927-3037.2013.02.04.3","DOIUrl":"https://doi.org/10.6000/1927-3037.2013.02.04.3","url":null,"abstract":"Acanthamoeba Keratitis is an important corneal infection that caused impaired vision. The Universiti Malaysia Terengganu (UMT) researcher found new species of Acanthamoeba that was being isolated from eye infection in Hospital Kuala Lumpur, which have the characteristic between Acanthamoeba polyphaga and A. castellanii named as HKL-Acantha. This study was aimed to generate specific monoclonal antibody against HKL-Acantha that eventually could be used in diagnosing eye infection among Malaysian. Two Balb/c mice were immunized with sonicated HKL-Acantha through intraperitoneal injection, and anti HKL-acantha IgG, IgM, IgA were measured using ELISA test. Sera from infected mice showed detectable anti-HKL-acantha IgM. The spleen from animal with high antibody titer around (1:81000) was used as a partner in fusion with SP2/0-Ag14 myeloma cells to produce new hybridoma cells, which were then selected and cloned using the selection medium. Two positive hybridoma clones secreting IgM antibodies were obtained and named as hybridoma UTM-Ha1 and UTM-Ha2. Both clones were secreting monoclonal IgM antibody specific to HKL-Acantha. This study suggests the potential of both hybridomas UTM-Ha1 and UTM-Ha2 to generate specific monoclonal IgM against HKL-acantha. This newly generated monoclonal IgM could be used to diagnose the presence of HKL- acantha in patients with eye infection in future.","PeriodicalId":90181,"journal":{"name":"International journal of biotechnology for wellness industries","volume":"2 1","pages":"164-172"},"PeriodicalIF":0.0,"publicationDate":"2013-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71248744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Potential of Hydrocyclone Application for Mammalian Cell Separation in Perfusion Cultivation Bioreactors 水力旋流分离器在灌注培养生物反应器中分离哺乳动物细胞的应用前景

International journal of biotechnology for wellness industries Pub Date : 2013-12-31 DOI: 10.6000/1927-3037.2013.02.04.2

E. A. Elsayed, M. Wadaan

{"title":"The Potential of Hydrocyclone Application for Mammalian Cell Separation in Perfusion Cultivation Bioreactors","authors":"E. A. Elsayed, M. Wadaan","doi":"10.6000/1927-3037.2013.02.04.2","DOIUrl":"https://doi.org/10.6000/1927-3037.2013.02.04.2","url":null,"abstract":"Hydrocyclones have been traditionally applied for long times in many industrial fields, such as in mineral processing and mining, chemical and petrochemical, and food industries. They have many characteristics that favor them as separation system in solid/liquid, gas/liquid and liquid/liquid processes. During the last two decades, they have been evaluated for their possible application in the separation of microbial and mammalian cells. Nowadays, mammalian cells are widely used for the production of a large number of valuable therapeutic proteins, antibodies, hormones and vaccines. This review highlights the potential of the application of hydrocyclones for mammalian cell separation in continuous perfusion biorecators. The discussion will cover the structure of hydrocyclone, mechanism of separation inside hydrocyclones, different theories describing the separation process, as well as the effect of changing different geometrical variables on the efficiency and performance of the separation process. Furthermore, we will focus on the latest developments achieved in the field of separation of living cells in both laboratory and pilot plant cultivation scales.","PeriodicalId":90181,"journal":{"name":"International journal of biotechnology for wellness industries","volume":"2 1","pages":"153-163"},"PeriodicalIF":0.0,"publicationDate":"2013-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71249156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Colon Cancer Reversed by Phyto-Nutritional Therapy: A Case Study 植物营养疗法逆转结肠癌:一个案例研究

International journal of biotechnology for wellness industries Pub Date : 2013-09-30 DOI: 10.6000/1927-3037.2013.02.03.4

S. Yap

{"title":"Colon Cancer Reversed by Phyto-Nutritional Therapy: A Case Study","authors":"S. Yap","doi":"10.6000/1927-3037.2013.02.03.4","DOIUrl":"https://doi.org/10.6000/1927-3037.2013.02.03.4","url":null,"abstract":"Colorectal cancer is the second most common cancer in Malaysia and up to 80% of its patients seek complementary therapies. Globally, up to 95% of cancer patient use adjunct therapies to reduce chemotherapy-induced side-effects. Morgan et al . (2004) had showed that conventional treatment for colon cancer produced minimal results. The vast majority of cancer survivors admitted to using dietary supplements and herbs after their cancer diagnosis. Current treatment seems to pay more attention to cancer as a disease rather than meeting the physiological needs of the patient. Nutritional therapy should be individually tailored since dietary deficiency is multifactorial and the tumor burden for each patient is different. Cancer is a metabolic disorder and colon cancer is strongly associated with advancing age, dietary and lifestyle habits. Up to 90% of all cancers might be prevented with proper nutrition since one-third of overall cancer deaths are linked to malnutrition. This case study shows how a late stage colon adenocarcinoma might be completely reversed by evidence-based phyto-nutritional therapy combined with some lifestyle modifications. The cancer apoptotic properties of this natural protocol warrant further investigation involving a higher number of patients with similar conditions","PeriodicalId":90181,"journal":{"name":"International journal of biotechnology for wellness industries","volume":"2 1","pages":"132-139"},"PeriodicalIF":0.0,"publicationDate":"2013-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71248267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Development of a High-Throughput Diagnosis Method for Detecting the ALDH2 Gene Using Fingernail DNA 利用指甲DNA检测ALDH2基因的高通量诊断方法的建立

International journal of biotechnology for wellness industries Pub Date : 2013-09-30 DOI: 10.6000/1927-3037.2013.02.03.1

E. Nagayoshi, Y. Mizuta, Y. Takii

{"title":"Development of a High-Throughput Diagnosis Method for Detecting the ALDH2 Gene Using Fingernail DNA","authors":"E. Nagayoshi, Y. Mizuta, Y. Takii","doi":"10.6000/1927-3037.2013.02.03.1","DOIUrl":"https://doi.org/10.6000/1927-3037.2013.02.03.1","url":null,"abstract":"A clinical method for effective genetic screening of the aldehyde dehydrogenase 2 (ALDH2) gene was developed, using the fingernail as a source of DNA material. A highly effective protease that could solubilize fingernail keratin and inactivate any DNase co-existing in the tissue was obtained by cloning and sequencing the gene for alkaline protease from Bacillus alcalophilus , followed by expression of the gene in Bacillus subtilis. The amino acid sequence of MIB029 protease contained common regions found in four other subtilisin-like proteases. In the fingernails of 113 female university students (average age 20.8 ± 0.7 years; body mass index, 20.4 ±1.6), ALDH2 frequency was 0.66 for the typical Glu homozygote, 0.32 for the heterozygote (Glu487Lys), and 0.020 for the atypical Lys homozygote. Through a questionnaire, it was found that the subjects had not previously received information regarding the relationship between their genetic background and consumption of alcoholic beverages. We found that the genetic single nucleotide polymorphism (SNP) background to alcoholism can be easily detected by collecting fingernails, which is convenient for subjects or patients.","PeriodicalId":90181,"journal":{"name":"International journal of biotechnology for wellness industries","volume":"2 1","pages":"91-100"},"PeriodicalIF":0.0,"publicationDate":"2013-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71248625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Carbon Dioxide Concentration on the Growth Response of Chlorella vulgaris Under Four Different Led Illumination 四种不同Led照明下二氧化碳浓度对小球藻生长响应的影响

International journal of biotechnology for wellness industries Pub Date : 2013-09-30 DOI: 10.6000/1927-3037.2013.02.03.3

S. Rendon, G. Roldan, R. Voroney

引用次数: 16

Fe(III)-Chloroquine Complex: A New Potent Compound in Wellness Industries of High Antimicrobial and Antimalarial Activities 铁(III)-氯喹配合物:在保健工业中具有高抗菌和抗疟活性的新有效化合物

International journal of biotechnology for wellness industries Pub Date : 2013-09-30 DOI: 10.6000/1927-3037.2013.02.03.5

D. Thakur, Jyotsna K. Mishra, K. S. Pitre, Smita Joshi

引用次数: 0