BMB Reports最新文献_第7页

Triamcinolone acetonide alleviates benign biliary stricture by ameliorating biliary fibrosis and inflammation. 曲安奈德通过改善胆道纤维化和炎症，缓解良性胆道狭窄。

IF 2.9 3区生物学

BMB Reports Pub Date : 2024-04-01

Seyeon Joo, See Young Lee, Su Yeon Lee, Yeseong Hwang, Minki Kim, Jae Woong Jeong, Sung Ill Jang, Sungsoon Fang

{"title":"Triamcinolone acetonide alleviates benign biliary stricture by ameliorating biliary fibrosis and inflammation.","authors":"Seyeon Joo, See Young Lee, Su Yeon Lee, Yeseong Hwang, Minki Kim, Jae Woong Jeong, Sung Ill Jang, Sungsoon Fang","doi":"","DOIUrl":"","url":null,"abstract":"We conducted a comprehensive series of molecular biological studies aimed at unraveling the intricate mechanisms underlying the anti-fibrotic effects of triamcinolone acetonide (TA) when used in conjunction with fully covered self-expandable metal stents (FCSEMS) for the management of benign biliary strictures (BBS). To decipher the molecular mechanisms responsible for the anti-fibrotic effects of corticosteroids on gallbladder mucosa, we conducted a comprehensive analysis. This analysis included various methodologies such as immunohistochemistry, ELISA, real-time PCR, and transcriptome analysis, enabling us to examine alterations in factors related to fibrosis and inflammation at both the protein and RNA levels. Overall, our findings revealed a dose-dependent decrease in fibrosisrelated signaling with higher TA concentrations. The 15 mg of steroid treatment (1X) exhibited anti-fibrosis and anti-inflammatory effects after 4 weeks, whereas the 30 mg of steroid treatment (2X) rapidly reduced fibrosis and inflammation within 2 weeks in BBS. Transcriptomic analysis results consistently demonstrated significant downregulation of fibrosis- and inflammation-related pathways and genes in steroid-treated fibroblasts. Use of corticosteroids, specifically TA, together with FCSEMS was effective for the treatment of BBS, ameliorating fibrosis and inflammation. Our molecular biological analysis supports the potential development of steroid-eluted FCSEMS as a therapeutic option for BBS in humans resulting from various surgical procedures. [BMB Reports 2024; 57(4): 200-205].","PeriodicalId":9010,"journal":{"name":"BMB Reports","volume":" ","pages":"200-205"},"PeriodicalIF":2.9,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11058357/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140206331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitofusin-2 enhances cervical cancer progression through Wnt/β-catenin signaling. Mitofusin-2 通过 Wnt/β-catenin 信号增强宫颈癌的进展。

IF 2.9 3区生物学

BMB Reports Pub Date : 2024-04-01

Sung Yong Ahn

{"title":"Mitofusin-2 enhances cervical cancer progression through Wnt/β-catenin signaling.","authors":"Sung Yong Ahn","doi":"","DOIUrl":"","url":null,"abstract":"Overexpression of mitofusin-2 (MFN2), a mitochondrial fusion protein, is frequently associated with poor prognosis in cervical cancer patients. Here, I aimed to investigate the involvement of MFN2 in cervical cancer progression and determine the effect of MFN2 on prognosis in cervical cancer patients. After generating MFN2-knockdown SiHa cells derived from squamous cell carcinoma, I investigated the effect of MFN2 on SiHa cell proliferation using the Cell Counting Kit-8 assay and determined the mRNA levels of proliferation markers. Colony-forming ability and tumorigenesis were evaluated using a colonyformation assay and tumor xenograft mouse models. The migratory and invasive abilities associated with MFN2 were measured using wound-healing and invasion assays. Wnt/β-cateninmediated epithelial-mesenchymal transition (EMT) markers related to MFN2 were assessed through quantitative RT-PCR. MFN2-knockdown SiHa cells exhibited reduced proliferation, colony formation, migration, invasion, and tumor formation in vivo. The motility of SiHa cells with MFN2 knockdown was reduced through Wnt/β-catenin-mediated EMT inhibition. MFN2 promoted cancer progression and tumorigenesis in SiHa cells. Overall, MFN2 could serve as a therapeutic target and a novel biomarker for cervical cancer. [BMB Reports 2024; 57(4): 194-199].","PeriodicalId":9010,"journal":{"name":"BMB Reports","volume":" ","pages":"194-199"},"PeriodicalIF":2.9,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11058360/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140048753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Targeting cell surface glucose-regulated protein 94 in gastric cancer with an anti-GRP94 human monoclonal antibody. 用抗 GRP94 人单克隆抗体靶向胃癌细胞表面葡萄糖调节蛋白 94。

IF 2.9 3区生物学

BMB Reports Pub Date : 2024-04-01

Hyun Jung Kim, Yea Bin Cho, Kyun Heo, Ji Woong Kim, Ha Gyeong Shin, Eun-Bi Lee, Seong-Min Park, Jong Bae Park, Sukmook Lee

{"title":"Targeting cell surface glucose-regulated protein 94 in gastric cancer with an anti-GRP94 human monoclonal antibody.","authors":"Hyun Jung Kim, Yea Bin Cho, Kyun Heo, Ji Woong Kim, Ha Gyeong Shin, Eun-Bi Lee, Seong-Min Park, Jong Bae Park, Sukmook Lee","doi":"","DOIUrl":"","url":null,"abstract":"Gastric cancer (GC), a leading cause of cancer-related mortality, remains a significant challenge despite recent therapeutic advancements. In this study, we explore the potential of targeting cell surface glucose-regulated protein 94 (GRP94) with antibodies as a novel therapeutic approach for GC. Our comprehensive analysis of GRP94 expression across various cancer types, with a specific focus on GC, revealed a substantial overexpression of GRP94, highlighting its potential as a promising target. Through in vitro and in vivo efficacy assessments, as well as toxicological analyses, we found that K101.1, a fully human monoclonal antibody designed to specifically target cell surface GRP94, effectively inhibits GC growth and angiogenesis without causing in vivo toxicity. Furthermore, our findings indicate that K101.1 promotes the internalization and concurrent downregulation of cell surface GRP94 on GC cells. In conclusion, our study suggests that cell surface GRP94 may be a potential therapeutic target in GC, and that antibody-based targeting of cell surface GRP94 may be an effective strategy for inhibiting GRP94-mediated GC growth and angiogenesis. [BMB Reports 2024; 57(4): 188-193].","PeriodicalId":9010,"journal":{"name":"BMB Reports","volume":" ","pages":"188-193"},"PeriodicalIF":2.9,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11058359/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140048754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Memory allocation at the neuronal and synaptic levels. 神经元和突触水平的记忆分配。

IF 2.9 3区生物学

BMB Reports Pub Date : 2024-04-01

HyoJin Park, Bong-Kiun Kaang

引用次数: 0

ErbB3 binding protein 1 contributes to adult hippocampal neurogenesis by modulating Bmp4 and Ascl1 signaling. ErbB3结合蛋白1通过调节Bmp4和Ascl1信号传导促进成年海马神经发生。

IF 2.9 3区生物学

BMB Reports Pub Date : 2024-04-01

Youngkwan Kim, Hyo Rim Ko, Inwoo Hwang, Jee-Yin Ahn

引用次数: 0

Calnexin as a dual-role biomarker: antibody-based diagnosis and therapeutic targeting in lung cancer. 作为双重角色生物标记物的 Calnexin：基于抗体的肺癌诊断和治疗靶点。

IF 3.8 3区生物学

BMB Reports Pub Date : 2024-03-01

Soyeon Lim, Youngeun Ha, Boram Lee, Junho Shin, Taiyoun Rhim

{"title":"Calnexin as a dual-role biomarker: antibody-based diagnosis and therapeutic targeting in lung cancer.","authors":"Soyeon Lim, Youngeun Ha, Boram Lee, Junho Shin, Taiyoun Rhim","doi":"","DOIUrl":"","url":null,"abstract":"Lung cancer carries one of the highest mortality rates among all cancers. It is often diagnosed at more advanced stages with limited treatment options compared to other malignancies. This study focuses on calnexin as a potential biomarker for diagnosis and treatment of lung cancer. Calnexin, a molecular chaperone integral to N-linked glycoprotein synthesis, has shown some associations with cancer. However, targeted therapeutic or diagnostic methods using calnexin have been proposed. Through 1D-LCMSMS, we identified calnexin as a biomarker for lung cancer and substantiated its expression in human lung cancer cell membranes using Western blotting, flow cytometry, and immunocytochemistry. Anti-calnexin antibodies exhibited complement-dependent cytotoxicity to lung cancer cell lines, resulting in a notable reduction in tumor growth in a subcutaneous xenograft model. Additionally, we verified the feasibility of labeling tumors through in vivo imaging using antibodies against calnexin. Furthermore, exosomal detection of calnexin suggested the potential utility of liquid biopsy for diagnostic purposes. In conclusion, this study establishes calnexin as a promising target for antibody-based lung cancer diagnosis and therapy, unlocking novel avenues for early detection and treatment. [BMB Reports 2024; 57(3): 155-160].","PeriodicalId":9010,"journal":{"name":"BMB Reports","volume":" ","pages":"155-160"},"PeriodicalIF":3.8,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10979343/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139671249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detecting DNA hydroxymethylation: exploring its role in genome regulation. 检测 DNA 羟甲基化：探索其在基因组调控中的作用。

IF 3.8 3区生物学

BMB Reports Pub Date : 2024-03-01

Sun-Min Lee

引用次数: 0

Whole-genome doubling is a double-edged sword: the heterogeneous role of whole-genome doubling in various cancer types. 全基因组加倍是一把双刃剑：全基因组加倍在不同癌症类型中的异质性作用。

IF 3.8 3区生物学

BMB Reports Pub Date : 2024-03-01

Eunhyong Chang, Joon-Yong An

{"title":"Whole-genome doubling is a double-edged sword: the heterogeneous role of whole-genome doubling in various cancer types.","authors":"Eunhyong Chang, Joon-Yong An","doi":"","DOIUrl":"","url":null,"abstract":"Whole-genome doubling (WGD), characterized by the duplication of an entire set of chromosomes, is commonly observed in various tumors, occurring in approximately 30-40% of patients with different cancer types. The effect of WGD on tumorigenesis varies depending on the context, either promoting or suppressing tumor progression. Recent advances in genomic technologies and large-scale clinical investigations have led to the identification of the complex patterns of genomic alterations underlying WGD and their functional consequences on tumorigenesis progression and prognosis. Our comprehensive review aims to summarize the causes and effects of WGD on tumorigenesis, highlighting its dualistic influence on cancer cells. We then introduce recent findings on WGD-associated molecular signatures and genetic aberrations and a novel subtype related to WGD. Finally, we discuss the clinical implications of WGD in cancer subtype classification and future therapeutic interventions. Overall, a comprehensive understanding of WGD in cancer biology is crucial to unraveling its complex role in tumorigenesis and identifying novel therapeutic strategies. [BMB Reports 2024; 57(3): 125-134].","PeriodicalId":9010,"journal":{"name":"BMB Reports","volume":" ","pages":"125-134"},"PeriodicalIF":3.8,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10979346/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140048755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genistein alleviates pulmonary fibrosis by inactivating lung fibroblasts. 染料木黄酮通过使肺成纤维细胞失活来减轻肺纤维化。

IF 3.8 3区生物学

BMB Reports Pub Date : 2024-03-01

Seung-Hyun Kwon, Hyunju Chung, Jung-Woo Seo, Hak Su Kim

{"title":"Genistein alleviates pulmonary fibrosis by inactivating lung fibroblasts.","authors":"Seung-Hyun Kwon, Hyunju Chung, Jung-Woo Seo, Hak Su Kim","doi":"","DOIUrl":"","url":null,"abstract":"Pulmonary fibrosis is a serious lung disease that occurs predominantly in men. Genistein is an important natural soybeanderived phytoestrogen that affects various biological functions, such as cell migration and fibrosis. However, the antifibrotic effects of genistein on pulmonary fibrosis are largely unknown. The antifibrotic effects of genistein were evaluated using in vitro and in vivo models of lung fibrosis. Proteomic data were analyzed using nano-LC-ESI-MS/MS. Genistein significantly reduced transforming growth factor (TGF)-β1-induced expression of collagen type I and α-smooth muscle actin (SMA) in MRC-5 cells and primary fibroblasts from patients with idiopathic pulmonary fibrosis (IPF). Genistein also reduced TGF-β1-induced expression of p-Smad2/3 and p-p38 MAPK in fibroblast models. Comprehensive protein analysis confirmed that genistein exerted an anti-fibrotic effect by regulating various molecular mechanisms, such as unfolded protein response, epithelial mesenchymal transition (EMT), mammalian target of rapamycin complex 1 (mTORC1) signaling, cell death, and several metabolic pathways. Genistein was also found to decrease hydroxyproline levels in the lungs of BLM-treated mice. Genistein exerted an anti-fibrotic effect by preventing fibroblast activation, suggesting that genistein could be developed as a pharmacological agent for the prevention and treatment of pulmonary fibrosis. [BMB Reports 2024; 57(3): 143-148].","PeriodicalId":9010,"journal":{"name":"BMB Reports","volume":" ","pages":"143-148"},"PeriodicalIF":3.8,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10979345/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41190372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the DNA methylome of Korean patients with colorectal cancer consolidates the clinical implications of cancer-associated methylation markers. 探索韩国结直肠癌患者的DNA甲基化组巩固了癌症相关甲基化标记的临床意义。

IF 3.8 3区生物学

BMB Reports Pub Date : 2024-03-01

Sejoon Lee, Kil-Yong Lee, Ji-Hwan Park, Duck-Woo Kim, Heung-Kwon Oh, Seong-Taek Oh, Jongbum Jeon, Dongyoon Lee, Soobok Joe, Hoang Bao Khanh Chu, Jisun Kang, Jin-Young Lee, Sheehyun Cho, Hyeran Shim, Si-Cho Kim, Hong Seok Lee, Young-Joon Kim, Jin Ok Yang, Jaeim Lee, Sung-Bum Kang

{"title":"Exploring the DNA methylome of Korean patients with colorectal cancer consolidates the clinical implications of cancer-associated methylation markers.","authors":"Sejoon Lee, Kil-Yong Lee, Ji-Hwan Park, Duck-Woo Kim, Heung-Kwon Oh, Seong-Taek Oh, Jongbum Jeon, Dongyoon Lee, Soobok Joe, Hoang Bao Khanh Chu, Jisun Kang, Jin-Young Lee, Sheehyun Cho, Hyeran Shim, Si-Cho Kim, Hong Seok Lee, Young-Joon Kim, Jin Ok Yang, Jaeim Lee, Sung-Bum Kang","doi":"","DOIUrl":"","url":null,"abstract":"Aberrant DNA methylation plays a critical role in the development and progression of colorectal cancer (CRC), which has high incidence and mortality rates in Korea. Various CRC-associated methylation markers for cancer diagnosis and prognosis have been developed; however, they have not been validated for Korean patients owing to the lack of comprehensive clinical and methylome data. Here, we obtained reliable methylation profiles for 228 tumor, 103 adjacent normal, and two unmatched normal colon tissues from Korean patients with CRC using an Illumina Infinium EPIC array; the data were corrected for biological and experiment biases. A comparative methylome analysis confirmed the previous findings that hypermethylated positions in the tumor were highly enriched in CpG island and promoter, 5' untranslated, and first exon regions. However, hypomethylated positions were enriched in the open-sea regions considerably distant from CpG islands. After applying a CpG island methylator phenotype (CIMP) to the methylome data of tumor samples to stratify the CRC patients, we consolidated the previously established clinicopathological findings that the tumors with high CIMP signatures were significantly enriched in the right colon. The results showed a higher prevalence of microsatellite instability status and MLH1 methylation in tumors with high CMP signatures than in those with low or non-CIMP signatures. Therefore, our methylome analysis and dataset provide insights into applying CRC-associated methylation markers for Korean patients regarding cancer diagnosis and prognosis. [BMB Reports 2024; 57(3): 161-166].","PeriodicalId":9010,"journal":{"name":"BMB Reports","volume":" ","pages":"161-166"},"PeriodicalIF":3.8,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10979344/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"107590064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0