IF 3.3 3区生物学

BMB Reports Pub Date : 2026-04-15

Ju Won Kim, Sanghyun Lee, Sejin Jeon, Sun-Woo Yoon, Yo Han Jang

{"title":"Stigmasterol elicits antiviral activity against influenza viruses through virucidal and entry inhibition activity in vitro","authors":"Ju Won Kim, Sanghyun Lee, Sejin Jeon, Sun-Woo Yoon, Yo Han Jang","doi":"","DOIUrl":"","url":null,"abstract":"Influenza viruses pose a significant global public health threat, leading to recurrent seasonal epidemics and occasional pandemics. The rising prevalence of drug-resistant strains highlights the urgent need for new antiviral agents with alternative mechanisms of action. Plant-derived natural products have emerged as promising antiviral resources due to their chemical diversity and wide range of biological activities. Stigmasterol, a naturally occurring phytosterol found in various plants, is recognized for its anti-inflammatory and antioxidant properties; however, its antiviral potential against influenza viruses has not been clearly established. In this study, we systematically assessed the antiviral activity of stigmasterol against influenza A and B viruses in vitro. Stigmasterol demonstrated significant virucidal activity against both virus types at non-cytotoxic concentrations and effectively interfered with the functions of the viral surface proteins hemagglutinin and neuraminidase. Using GFP-expressing reporter viruses and cytopathic effect reduction assays, we showed that stigmasterol robustly suppressed viral infection under pre-, co-, and post-treatment conditions. Importantly, stigmasterol inhibited viral entry through a mechanism independent of hemagglutinin inhibition. These findings provide the first evidence that stigmasterol has broad anti-influenza activity and support its potential as a plant-derived lead compound for developing novel therapeutic strategies against influenza infection.","PeriodicalId":9010,"journal":{"name":"BMB Reports","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147697520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enzyme-assisted extraction of plant-derived extracellular vesicles: recent advances, methodological considerations, and standardization needs. 酶辅助提取植物源性细胞外囊泡：最新进展，方法学考虑和标准化需求。

IF 3.3 3区生物学

BMB Reports Pub Date : 2026-04-06

Hyun Chul Jo, Seongje Hong, Kyung Oh Jung

{"title":"Enzyme-assisted extraction of plant-derived extracellular vesicles: recent advances, methodological considerations, and standardization needs.","authors":"Hyun Chul Jo, Seongje Hong, Kyung Oh Jung","doi":"","DOIUrl":"","url":null,"abstract":"Plant-derived extracellular vesicles (PDEVs) have attracted increasing attention as bioactive nanostructures with emerging applications in medicine, functional foods, and cosmetics. However, unlike mammalian extracellular vesicle systems, the recovery of PDEVs is fundamentally constrained by plant-specific structural features, particularly the rigid cell wall and the limited accessibility of vesicles within plant tissues. In plant systems, extracellular vesicles are thought to be retained, at least in part, within the apoplastic space between the plasma membrane and the cell wall, which restricts the direct application of conventional animal cell-based isolation workflows. Conventional extraction approaches based on mechanical disruption enable bulk recovery of vesicle-containing material but are frequently associated with intracellular contamination, cell wall-derived debris, increased extract viscosity, and poor process reproducibility. To address these limitations, recent studies have explored enzyme-based extraction strategies that partially relax the plant cell wall to facilitate the selective release of apoplastic vesicles while reducing nonspecific contamination. In this mini-review, we summarize recent advances in enzyme-based extraction strategies for PDEVs, with particular emphasis on their structural rationale, methodological advantages over mechanical disruption, and associated limitations. We further discuss key challenges in quantitative evaluation, purity assessment, and reporting standardization, highlighting the need for methodologically rigorous frameworks to enable reproducible isolation and reliable interpretation of PDEV-associated biological activities.","PeriodicalId":9010,"journal":{"name":"BMB Reports","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147621812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Loss of Tubby in MC4R- and VGLUT2-Expressing Neurons Impairs Energy Homeostasis. 表达MC4R-和vglut2的神经元中Tubby的缺失会损害能量稳态。

IF 3.3 3区生物学

BMB Reports Pub Date : 2026-04-06

Se Rok Jeong, Yong Taek Jeong, Chul Hoon Kim, Jeong Taeg Seo, Seok Jun Moon

引用次数: 0

Cereblon enhances hepatic hepcidin production through estrogen-related receptor gamma activation. 小脑通过激活雌激素相关受体增强肝磷脂的产生。

IF 3.3 3区生物学

BMB Reports Pub Date : 2026-04-06

Seungwon An, Chung Hyo Kang, Taehyun Park, Balachandar Nedumaran, Younjoo Sung, Taehoon Chung, Chul-Seung Park, Ali R Djalilian, Yong Deuk Kim

{"title":"Cereblon enhances hepatic hepcidin production through estrogen-related receptor gamma activation.","authors":"Seungwon An, Chung Hyo Kang, Taehyun Park, Balachandar Nedumaran, Younjoo Sung, Taehoon Chung, Chul-Seung Park, Ali R Djalilian, Yong Deuk Kim","doi":"","DOIUrl":"","url":null,"abstract":"Hepcidin (encoded by the HAMP gene), produced primarily by hepatocytes, is the master regulator of systemic iron homeostasis, and its dysregulation contributes to various iron-related metabolic disorders. Cereblon (CRBN) has been implicated in metabolic regulation, while estrogen-related receptor gamma (ESRRG) is known to govern energy homeostasis and mitochondrial function. Here, we demonstrate a novel CRBN-ESRRG signaling pathway that mediates endoplasmic reticulum (ER) stress-induced hepatic HAMP expression. In mice and primary hepatocytes exposed to tunicamycin-induced ER stress, gene expression and biochemical analyses revealed that hepatic Crbn, Esrrg, and Hamp transcript levels were significantly increased. Hepcidin protein levels were correspondingly elevated, accompanied by reduced serum iron levels and increased cellular iron levels, consistent with hepcidin-mediated regulation of iron distribution. Overexpression of Crbn enhanced ESRRG expression and increased hepatic hepcidin production, whereas Crbn or Esrrg knockdown attenuated this response. Chromatin immunoprecipitation assays demonstrated enhanced ESRRG recruitment to the Hamp promoter. Collectively, these findings identify a CRBN-ESRRG regulatory axis that drives hepatic HAMP expression under ER stress and suggest a potential therapeutic target for ER stress-associated metabolic and iron disorders.","PeriodicalId":9010,"journal":{"name":"BMB Reports","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147621803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitochondrial transplantation restores mitochondrial content and function in SSBP1-related mitochondrial DNA depletion syndrome. 线粒体移植可恢复ssbp1相关线粒体DNA缺失综合征的线粒体含量和功能。

IF 3.3 3区生物学