IF 3.3 3区生物学

BMB Reports Pub Date : 2025-09-29

YongJin Cheon, Hyeonjeong Hwang, Kyuhyung Kim

{"title":"Caenorhabditis elegans avoids epigallocatechin gallate (EGCG) through SRXA-7 G-protein coupled receptor.","authors":"YongJin Cheon, Hyeonjeong Hwang, Kyuhyung Kim","doi":"","DOIUrl":"","url":null,"abstract":"Flavonoids are plant-derived polyphenols that influence nematode behavior, yet their neuronal and molecular targets remain poorly understood. Here, we show that the free-living nematode Caenorhabditis elegans detects and avoids a green tea catechin epigallocatechin gallate (EGCG) via the SRXA-7 Gprotein coupled receptor (GPCR) in the ASH nociceptive neurons. EGCG and epicatechin gallate (ECG), both containing a galloyl group, trigger strong avoidance, unlike other green tea catechins lacking this moiety. EGCG avoidance behavior displays species- and strain-specific differences among Caenorhabditis species and wild C. elegans isolates. Moreover, it is dynamically modulated by prior experience and feeding state. The ASH chemosensory neurons are required for EGCG detection, with avoidance mediated through canonical GPCR signaling components including GRK-2, GPA-3, ODR-3, and TRPV channels OCR-2 and OSM-9. A targeted GPCR screen revealed that srxa-7 mutants exhibited specific defects in EGCG avoidance. EGCG-evoked calcium responses in ASH are reduced in srxa-7 mutants and restored by ASH-specific expression of srxa-7 cDNA. These findings indicate that SRXA-7 is a sensory receptor for galloylated polyphenols, uncovering the neuronal and molecular basis of adaptive aversive responses to dietary plant compounds in nematodes.","PeriodicalId":9010,"journal":{"name":"BMB Reports","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145184582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deep learning application for genomic data analysis. 基因组数据分析的深度学习应用。

IF 3.3 3区生物学

BMB Reports Pub Date : 2025-09-16

Chang Beom Jeong, Hyein Cho, Daechan Park

引用次数: 0

Redefining preclinical testing: human-relevant alternatives beyond animal models. 重新定义临床前试验：超越动物模型的与人类相关的替代方法。

IF 3.3 3区生物学

BMB Reports Pub Date : 2025-09-16

Jieun Jang, Wonjun Yang, Hanseul Yang, Nam-Kyung Lee, Jong-Gil Park, Wantae Kim, Boksik Cha, Sung-Jin Yoon, Ji-Yoon Noh, Jangwook Lee

{"title":"Redefining preclinical testing: human-relevant alternatives beyond animal models.","authors":"Jieun Jang, Wonjun Yang, Hanseul Yang, Nam-Kyung Lee, Jong-Gil Park, Wantae Kim, Boksik Cha, Sung-Jin Yoon, Ji-Yoon Noh, Jangwook Lee","doi":"","DOIUrl":"","url":null,"abstract":"Both ethical imperatives and scientific limitations increasingly challenge the traditional reliance on animal models for toxicity testing and drug evaluation, particularly in the era of precision medicine. In response, a paradigm shift is underway, marked by the development of advanced in vitro and in silico technologies that can offer human-relevant and mechanistically informed alternatives. This review examines well-established alternatives, such as receptor binding assays, the monocyte activation test, and enzyme-linked immunosorbent assays, highlighting their applications, mechanisms, and limitations. We further explore emerging human-relevant technologies that include organoids, organ-on-a-chip systems, microphysiological systems, and artificial intelligence-powered modeling platforms. Special emphasis is placed on immune-integrated microphysiological systems as next-generation platforms to evaluate immunotherapy, vaccine responses, and immune toxicities. These models recapitulate dynamic human physiological processes, such as hematopoiesis and germinal center reactions, beyond the capabilities of traditional animal systems. Collectively, these technologies represent scientifically superior and ethically progressive trajectories for preclinical testing. Their integration into regulatory and industrial workflows requires continued refinement, cross-sector collaboration, and standardization.","PeriodicalId":9010,"journal":{"name":"BMB Reports","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145079640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AQP1/4, CLIC5 Dysregulation and lipid metabolism alterations in lung cancer. 肺癌中AQP1/4、CLIC5失调和脂质代谢改变。

IF 3.3 3区生物学

BMB Reports Pub Date : 2025-09-16

Heung-Seok Bae, Je-Yoel Cho

{"title":"AQP1/4, CLIC5 Dysregulation and lipid metabolism alterations in lung cancer.","authors":"Heung-Seok Bae, Je-Yoel Cho","doi":"","DOIUrl":"","url":null,"abstract":"To advance the development of novel therapies for lung cancer, we investigated tumor-associated molecules implicated in tumorigenesis. RNA-seq data were generated from paired tumors and adjacent normal tissues of four patients with lung squamous cell carcinoma (LUSC) and five patients with lung adenocarcinoma (LUAD). Additional analyses utilized RNA-seq data from The Cancer Genome Atlas (TCGA), including paired tumor and adjacent normal samples (51 LUSC, 57 LUAD) and tumor-only samples (450 LUSC, 461 LUAD). Adjacent normal tissues served as controls. Our RNA-seq results showed strong concordance with TCGA data. Ion channels Aqp1, Aqp4, and Clic5 were significantly downregulated in lung tumors, whereas enzymes involved in membrane lipid metabolism, including phosphatidylcholine (PC), sphingomyelin (SM), and cholesterol (Cho), were upregulated in lung tumors. Cardiolipin (CL), a mitochondrial inner membrane lipid, was downregulated in lung tumors. These changes might have impaired oxygen permeability and mitochondrial function, promoting hypoxia and reactive oxygen species (ROS) production. Hif1α expression was elevated in both LUSC and LUAD, along with a hypoxiaresponsive protein kinase Csnk2a1 and its downstream targets Hdac1 and Hdac2. ROS-responsive transcription factors Yy1, Foxm1, E2f1, and E2f8 were also significantly upregulated in both LUSC and LUAD. Notably, the master epigenetic regulator Uhrf1 activated by these transcription factors showed marked overexpression in tumors compared to that in normal tissues. TCGA data corroborated these findings. Our study identified tumor cell membrane-associated molecules, including ion channels (Aqp1, Aqp4, Clic5) and membrane lipid metabolism enzymes (PC, SM, Cho, and CL), as critical contributors to lung tumorigenesis. These molecules represent promising targets for developing innovative anti-cancer therapies.","PeriodicalId":9010,"journal":{"name":"BMB Reports","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145079588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Toward unraveling molecular grammars for dsRNA-binding proteins: substrate recognition to binding mechanisms. 解开dsrna结合蛋白的分子语法：底物识别到结合机制。

IF 3.3 3区生物学

BMB Reports Pub Date : 2025-09-16

Jaemin Jeon, Yoosik Kim

引用次数: 0

Systems memory consolidation during sleep: oscillations, neuromodulators, and synaptic remodeling. 睡眠期间的系统记忆巩固：振荡、神经调节剂和突触重塑。

IF 3.3 3区生物学

BMB Reports Pub Date : 2025-09-16

Jaekyung Kim, Minjeong Park

{"title":"Systems memory consolidation during sleep: oscillations, neuromodulators, and synaptic remodeling.","authors":"Jaekyung Kim, Minjeong Park","doi":"","DOIUrl":"","url":null,"abstract":"Memory consolidation transforms newly acquired experiences into stable long-term memories essential for learning and cognition. This process involves systems consolidation, where memory traces are reorganized across brain regions, and synaptic consolidation, which fine-tunes local neural connections. Sleep plays a critical role in both, coordinating memory reactivation, synaptic remodeling, and long-range neural communication. Systems consolidation is supported by stagespecific brain oscillations: during NREM sleep, the coupling of slow-oscillations, spindles, and sharp-wave ripples facilitates hippocampal-cortical transfer of memory representations, while REM sleep theta oscillations contribute to memory integration, abstraction, and emotional tagging. Complementary neuromodulatory dynamics, particularly involving norepinephrine and dopamine, regulate the timing and prioritization of memory processing. At the synaptic level, sleep balances strengthening and weakening of connections through a coordinated interplay of NREM and REM activity. Recent findings also suggest that dreaming may reflect the subjective correlate of these processes, particularly through the integration of recent and remote memory fragments. Although the precise relationship between systems-level reorganization and local synaptic refinement remains unclear-partly due to current technical limitations-emerging approaches are beginning to bridge these scales. Together, these findings underscore the integrative role of sleep in optimizing memory consolidation and offer promising avenues for clinical and translational research.","PeriodicalId":9010,"journal":{"name":"BMB Reports","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145079645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lefty2 prevents RANKL-induced bone loss by inhibiting osteoclast differentiation. Lefty2通过抑制破骨细胞分化来阻止rankl诱导的骨质流失。

IF 3.3 3区生物学

BMB Reports Pub Date : 2025-09-16

Jung Ha Kim, Kabsun Kim, Inyoung Kim, Semun Seong, Nacksung Kim

引用次数: 0

N-acetyl-L-alanine ameliorates atopic dermatitis-like symptoms by suppressing Th2 differentiation in DNFB-induced NC/Nga mice. n -乙酰-l -丙氨酸通过抑制dnfb诱导的NC/Nga小鼠Th2分化来改善特应性皮炎样症状。

IF 3.3 3区生物学

BMB Reports Pub Date : 2025-09-08

Uyanga TSogt, Seung-Gook Kim, Suhong Duan, Hyung-Jin Lim, Jiaying Bao, Hwa-Ryung Song, Jong-Suk Kim, Myung-Kwan Han

{"title":"N-acetyl-L-alanine ameliorates atopic dermatitis-like symptoms by suppressing Th2 differentiation in DNFB-induced NC/Nga mice.","authors":"Uyanga TSogt, Seung-Gook Kim, Suhong Duan, Hyung-Jin Lim, Jiaying Bao, Hwa-Ryung Song, Jong-Suk Kim, Myung-Kwan Han","doi":"","DOIUrl":"","url":null,"abstract":"Atopic dermatitis (AD) is a chronic dermatological disorder characterized by intense pruritus and eczematous lesions. Repeated topical application of 2,4-dinitrofluorobenzene (DNFB) in NC/Nga mice produces AD-like clinical symptoms that closely resemble human AD. N-Acetyl-L-Alanine (L-NAA), a derivative of L-Alanine, has unknown biological and physiological effects on cutaneous tissue. In this study, we investigated whether L-NAA modifies AD-like symptoms elicited by ongoing DNFB exposure in NC/Nga mice. Topical administration of L-NAA markedly attenuated the development of AD-like cutaneous lesions triggered by DNFB. L-NAA treatment further suppressed DNFB-induced infiltration of eosinophils and mast cells and prevented the increase of serum IgE resulting from DNFB application. L-NAA treatment decreased DNFB-stimulated expression of IL-4, a Th2-associated cytokine, but increased IFN-γ expression, indicative of Th1 activity, within the skin lesions. In addition, L-NAA prevented the DNFB-driven upregulation of GATA3, a central regulator of Th2 lineage differentiation, in CD4＋ cells, with no effect on T-bet, the principal regulator of Th1 cells. These findings indicate that L-NAA can limit Th2 differentiation in the AD mouse model. Therefore, L-NAA may serve as a promising therapeutic and immunomodulatory compound against AD.","PeriodicalId":9010,"journal":{"name":"BMB Reports","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145013802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

APC/C-CDH1 suppresses AROS-mediated protection against DNA damage-induced senescence by ubiquitination. APC/C-CDH1通过泛素化抑制aros介导的DNA损伤诱导的衰老保护。

IF 3.3 3区生物学

BMB Reports Pub Date : 2025-09-08

Ji-Hye Yang, Hanbyeul Choi, Seung Baek Lee, Soo-Jong Um, Eun-Joo Kim

引用次数: 0

Functional analysis of secreted tissue inhibitor of metalloproteinases-1 from adult human neural stem cells (ahNSCs) for regeneration and neuroprotection. 成体人神经干细胞（ahNSCs）分泌的金属蛋白酶组织抑制剂-1在再生和神经保护中的功能分析。

IF 3.3 3区生物学

BMB Reports Pub Date : 2025-09-08

Ki Hoon Kim, Dong Geun Hong, Hye Yun Chae, Dong Oh Kim, So Heun Lee, Chung Kwon Kim, Geun-Hyoung Ha, Kyeung Min Joo

{"title":"Functional analysis of secreted tissue inhibitor of metalloproteinases-1 from adult human neural stem cells (ahNSCs) for regeneration and neuroprotection.","authors":"Ki Hoon Kim, Dong Geun Hong, Hye Yun Chae, Dong Oh Kim, So Heun Lee, Chung Kwon Kim, Geun-Hyoung Ha, Kyeung Min Joo","doi":"","DOIUrl":"","url":null,"abstract":"The adult human neural stem cell (ahNSC)-conditioned medium (CM) contains various secreted factors that promote tissue repair and neuroprotection. This study aimed to identify the key secreted proteins in ahNSC-CM and investigate the role of tissue inhibitor of metalloproteinases-1 (TIMP-1) in wound healing, angiogenesis, and neuroprotection against oxygenglucose deprivation. Cytokine array and liquid chromatography- tandem mass spectrometry analysis of ahNSC-CM revealed that monocyte chemoattractant protein-1 (MCP-1) and TIMP-1 were highly abundant. Enzyme-linked immunosorbent assay confirmed the enrichment of both in ahNSC-CM. Recombinant human TIMP-1 promoted wound closure and angiogenesis, whereas neutralizing TIMP-1 in ahNSC-CM attenuated these effects, with wound healing involving the extracellular signalregulated kinases 1 and 2 and protein kinase B pathways. Furthermore, ahNSC-CM protected neurons from oxygen-glucose deprivation-induced apoptosis, and this neuroprotective effect was partially reversed by TIMP-1 neutralization, potentially through the modulation of B-cell lymphoma 2 and B-cell lymphoma 2-associated X protein expression. Consistent with findings in ahNSCs, where monocyte chemoattractant protein-1 is highly expressed and contributes to pro-angiogenic effects, our study highlights the importance of secreted factors such as TIMP-1 in the therapeutic potential of ahNSC-CM. These findings suggest that TIMP-1 is a significant component of ahNSC-CM and contributes to its regenerative and neuroprotective properties.","PeriodicalId":9010,"journal":{"name":"BMB Reports","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145013769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

BMB Reports最新文献