BMB Reports最新文献_第6页

CDKN2 expression is a potential biomarker for T cell exhaustion in hepatocellular carcinoma. CDKN2 表达是肝细胞癌中 T 细胞衰竭的潜在生物标志物。

IF 2.9 3区生物学

BMB Reports Pub Date : 2024-06-01

Shibo Wei, Yan Zhang, Baeki E Kang, Wonyoung Park, He Guo, Seungyoon Nam, Jong-Sun Kang, Jee-Heon Jeong, Yunju Jo, Dongryeol Ryu, Yikun Jiang, Ki-Tae Ha

{"title":"CDKN2 expression is a potential biomarker for T cell exhaustion in hepatocellular carcinoma.","authors":"Shibo Wei, Yan Zhang, Baeki E Kang, Wonyoung Park, He Guo, Seungyoon Nam, Jong-Sun Kang, Jee-Heon Jeong, Yunju Jo, Dongryeol Ryu, Yikun Jiang, Ki-Tae Ha","doi":"","DOIUrl":"","url":null,"abstract":"Hepatocellular Carcinoma (HCC), the predominant primary hepatic malignancy, is the prime contributor to mortality. Despite the availability of multiple surgical interventions, patient outcomes remain suboptimal. Immunotherapies have emerged as effective strategies for HCC treatment with multiple clinical advantages. However, their curative efficacy is not always satisfactory, limited by the dysfunctional T cell status. Thus, there is a pressing need to discover novel potential biomarkers indicative of T cell exhaustion (Tex) for personalized immunotherapies. One promising target is Cyclin-dependent kinase inhibitor 2 (CDKN2) gene, a key cell cycle regulator with aberrant expression in HCC. However, its specific involvement remains unclear. Herein, we assessed the potential of CDKN2 expression as a promising biomarker for HCC progression, particularly for exhausted T cells. Our transcriptome analysis of CDKN2 in HCC revealed its significant role involving in HCC development. Remarkably, single-cell transcriptomic analysis revealed a notable correlation between CDKN2 expression, particularly CDKN2A, and Tex markers, which was further validated by a human cohort study using human HCC tissue microarray, highlighting CDKN2 expression as a potential biomarker for Tex within the intricate landscape of HCC progression. These findings provide novel perspectives that hold promise for addressing the unmet therapeutic need within HCC treatment. [BMB Reports 2024; 57(6): 287-292].","PeriodicalId":9010,"journal":{"name":"BMB Reports","volume":" ","pages":"287-292"},"PeriodicalIF":2.9,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11214889/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140206329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emerging paradigms in cancer cell plasticity. 癌细胞可塑性的新范例。

IF 2.9 3区生物学

BMB Reports Pub Date : 2024-06-01

Hyunbin D Huh, Hyun Woo Park

{"title":"Emerging paradigms in cancer cell plasticity.","authors":"Hyunbin D Huh, Hyun Woo Park","doi":"","DOIUrl":"","url":null,"abstract":"Cancer cells metastasize to distant organs by altering their characteristics within the tumor microenvironment (TME) to effectively overcome challenges during the multistep tumorigenesis. Plasticity endows cancer cell with the capacity to shift between different morphological states to invade, disseminate, and seed metastasis. The epithelial-to-mesenchymal transition (EMT) is a theory derived from tissue biopsy, which explains the acquisition of EMT transcription factors (TFs) that convey mesenchymal features during cancer migration and invasion. On the other hand, adherent-to-suspension transition (AST) is an emerging theory derived from liquid biopsy, which describes the acquisition of hematopoietic features by AST-TFs that reprograms anchorage dependency during the dissemination of circulating tumor cells (CTCs). The induction and plasticity of EMT and AST dynamically reprogram cell-cell interaction and cell-matrix interaction during cancer dissemination and colonization. Here, we review the mechanisms governing cellular plasticity of AST and EMT during the metastatic cascade and discuss therapeutic challenges posed by these two morphological adaptations to provide insights for establishing new therapeutic interventions. [BMB Reports 2024; 57(6): 273-280].","PeriodicalId":9010,"journal":{"name":"BMB Reports","volume":" ","pages":"273-280"},"PeriodicalIF":2.9,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11214895/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140874453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cisd2 deficiency impairs neutrophil function by regulating calcium homeostasis via Calnexin and SERCA. Cisd2 缺乏症通过 Calnexin 和 SERCA 调节钙稳态，从而损害中性粒细胞的功能。

IF 2.9 3区生物学

BMB Reports Pub Date : 2024-05-01

Un Yung Choi, Youn Jung Choi, Shin-Ae Lee, Ji-Seung Yoo

{"title":"Cisd2 deficiency impairs neutrophil function by regulating calcium homeostasis via Calnexin and SERCA.","authors":"Un Yung Choi, Youn Jung Choi, Shin-Ae Lee, Ji-Seung Yoo","doi":"","DOIUrl":"","url":null,"abstract":"In the context of aging, the susceptibility to infectious diseases increases, leading to heightened morbidity and mortality. This phenomenon, termed immunosenescence, is characterized by dysregulation in the aging immune system, including abnormal alterations in lymphocyte composition, elevated basal inflammation, and the accumulation of senescent T cells. Such changes contribute to increased autoimmune diseases, enhanced infection severity, and reduced responsiveness to vaccines. Utilizing aging animal models becomes imperative for a comprehensive understanding of immunosenescence, given the complexity of aging as a physiological process in living organisms. Our investigation focuses on Cisd2, a causative gene for Wolfram syndrome, to elucidate on immunosenescence. Cisd2 knockout (KO) mice, serving as a model for premature aging, exhibit a shortened lifespan with early onset of aging-related features, such as decreased bone density, hair loss, depigmentation, and optic nerve degeneration. Intriguingly, we found that the Cisd2 KO mice present a higher number of neutrophils in the blood; however, isolated neutrophils from these mice display functional defects. Through mass spectrometry analysis, we identified an interaction between Cisd2 and Calnexin, a protein known for its role in protein quality control. Beyond this function, Calnexin also regulates calcium homeostasis through interaction with sarcoendoplasmic reticulum calcium transport ATPase (SERCA). Our study proposes that Cisd2 modulates calcium homeostasis via its interaction with Calnexin and SERCA, consequently influencing neutrophil functions. [BMB Reports 2024; 57(5): 256-261].","PeriodicalId":9010,"journal":{"name":"BMB Reports","volume":" ","pages":"256-261"},"PeriodicalIF":2.9,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11139677/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140847976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

3D epigenomics and 3D epigenopathies. 三维表观基因组学和三维表观基因病。

IF 2.9 3区生物学

BMB Reports Pub Date : 2024-05-01

Kyung-Hwan Lee, Jungyu Kim, Ji Hun Kim

引用次数: 0

BAP1 controls mesenchymal stem cell migration by inhibiting the ERK signaling pathway. BAP1通过抑制ERK信号通路控制间充质干细胞迁移。

IF 2.9 3区生物学

BMB Reports Pub Date : 2024-05-01

Seobin Kim, Eun-Woo Lee, Doo-Byoung Oh, Jinho Seo

{"title":"BAP1 controls mesenchymal stem cell migration by inhibiting the ERK signaling pathway.","authors":"Seobin Kim, Eun-Woo Lee, Doo-Byoung Oh, Jinho Seo","doi":"","DOIUrl":"","url":null,"abstract":"Due to their stem-like characteristics and immunosuppressive properties, Mesenchymal stem cells (MSCs) offer remarkable potential in regenerative medicine. Much effort has been devoted to enhancing the efficacy of MSC therapy by enhancing MSC migration. In this study, we identified deubiquitinase BRCA1- associated protein 1 (BAP1) as an inhibitor of MSC migration. Using deubiquitinase siRNA library screening based on an in vitro wound healing assay, we found that silencing BAP1 significantly augmented MSC migration. Conversely, BAP1 overexpression reduced the migration and invasion capabilities of MSCs. BAP1 depletion in MSCs upregulates ERK phosphorylation, thereby increasing the expression of the migration factor, osteopontin. Further examination revealed that BAP1 interacts with phosphorylated ERK1/2, deubiquitinating their ubiquitins, and thus attenuating the ERK signaling pathway. Overall, our study highlights the critical role of BAP1 in regulating MSC migration through its deubiquitinase activity, and suggests a novel approach to improve the therapeutic potential of MSCs in regenerative medicine. [BMB Reports 2024; 57(5): 250-255].","PeriodicalId":9010,"journal":{"name":"BMB Reports","volume":" ","pages":"250-255"},"PeriodicalIF":2.9,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11139679/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"107590063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-cell RNA sequencing reveals the heterogeneity of adipose tissue-derived mesenchymal stem cells under chondrogenic induction. 单细胞RNA测序揭示了软骨诱导下脂肪组织来源的间充质干细胞的异质性。

IF 2.9 3区生物学

BMB Reports Pub Date : 2024-05-01

Jeewan Chun, Ji-Hoi Moon, Kyu Hwan Kwack, Eun-Young Jang, Saebyeol Lee, Hak Kyun Kim, Jae-Hyung Lee

{"title":"Single-cell RNA sequencing reveals the heterogeneity of adipose tissue-derived mesenchymal stem cells under chondrogenic induction.","authors":"Jeewan Chun, Ji-Hoi Moon, Kyu Hwan Kwack, Eun-Young Jang, Saebyeol Lee, Hak Kyun Kim, Jae-Hyung Lee","doi":"","DOIUrl":"","url":null,"abstract":"This study investigated how adipose tissue-derived mesenchymal stem cells (AT-MSCs) respond to chondrogenic induction using droplet-based single-cell RNA sequencing (scRNA-seq). We analyzed 37,219 high-quality transcripts from control cells and cells induced for 1 week (1W) and 2 weeks (2W). Four distinct cell clusters (0-3), undetectable by bulk analysis, exhibited varying proportions. Cluster 1 dominated in control and 1W cells, whereas clusters (3, 2, and 0) exclusively dominated in control, 1W, and 2W cells, respectively. Furthermore, heterogeneous chondrogenic markers expression within clusters emerged. Gene ontology (GO) enrichment analysis of differentially expressed genes unveiled cluster-specific variations in key biological processes (BP): (1) Cluster 1 exhibited up-regulation of GO-BP terms related to ribosome biogenesis and translational control, crucial for maintaining stem cell properties and homeostasis; (2) Additionally, cluster 1 showed up-regulation of GO-BP terms associated with mitochondrial oxidative metabolism; (3) Cluster 3 displayed up-regulation of GO-BP terms related to cell proliferation; (4) Clusters 0 and 2 demonstrated similar up-regulation of GO-BP terms linked to collagen fibril organization and supramolecular fiber organization. However, only cluster 0 showed a significant decrease in GO-BP terms related to ribosome production, implying a potential correlation between ribosome regulation and the differentiation stages of AT-MSCs. Overall, our findings highlight heterogeneous cell clusters with varying balances between proliferation and differentiation before, and after, chondrogenic stimulation. This provides enhanced insights into the single-cell dynamics of AT-MSCs during chondrogenic differentiation. [BMB Reports 2024; 57(5): 232-237].","PeriodicalId":9010,"journal":{"name":"BMB Reports","volume":" ","pages":"232-237"},"PeriodicalIF":2.9,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11139680/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71420408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MMPP is a novel VEGFR2 inhibitor that suppresses angiogenesis via VEGFR2/AKT/ERK/NF-κB pathway. MMPP是一种新型的VEGFR2抑制剂，通过VEGFR2/AKT/ERK/NF-κB途径抑制血管生成。

IF 2.9 3区生物学

BMB Reports Pub Date : 2024-05-01

Na-Yeon Kim, Hyo-Min Park, Jae-Young Park, Uijin Kim, Ha Youn Shin, Hee Pom Lee, Jin Tae Hong, Do-Young Yoon

{"title":"MMPP is a novel VEGFR2 inhibitor that suppresses angiogenesis via VEGFR2/AKT/ERK/NF-κB pathway.","authors":"Na-Yeon Kim, Hyo-Min Park, Jae-Young Park, Uijin Kim, Ha Youn Shin, Hee Pom Lee, Jin Tae Hong, Do-Young Yoon","doi":"","DOIUrl":"","url":null,"abstract":"Many types of cancer are associated with excessive angiogenesis. Anti-angiogenic treatment is an effective strategy for treating solid cancers. This study aimed to demonstrate the inhibitory effects of (E)-2-methoxy-4-(3-(4-methoxyphenyl) prop-1-en-1-yl) phenol (MMPP) in VEGFA-induced angiogenesis. The results indicated that MMPP effectively suppressed various angiogenic processes, such as cell migration, invasion, tube formation, and sprouting of new vessels in human umbilical vein endothelial cells (HUVECs) and mouse aortic ring. The inhibitory mechanism of MMPP on angiogenesis involves targeting VEGFR2. MMPP showed high binding affinity for the VEGFR2 ATP-binding domain. Additionally, MMPP improved VEGFR2 thermal stability and inhibited VEGFR2 kinase activity, suppressing the downstream VEGFR2/AKT/ERK pathway. MMPP attenuated the activation and nuclear translocation of NF-κB, and it downregulated NF-κB target genes such as VEGFA, VEGFR2, MMP2, and MMP9. Furthermore, conditioned medium from MMPP-treated breast cancer cells effectively inhibited angiogenesis in endothelial cells. These results suggested that MMPP had great promise as a novel VEGFR2 inhibitor with potent anti-angiogenic properties for cancer treatment via VEGFR2/AKT/ERK/NF-κB signaling pathway. [BMB Reports 2024; 57(5): 244-249].","PeriodicalId":9010,"journal":{"name":"BMB Reports","volume":" ","pages":"244-249"},"PeriodicalIF":2.9,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11139683/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"107590067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gut microbiota-generated metabolites: missing puzzles to hosts' health, diseases, and aging. 肠道微生物群产生的代谢物：宿主健康、疾病和衰老的缺失之谜。

IF 2.9 3区生物学

BMB Reports Pub Date : 2024-05-01

Yan Zhang, Shibo Wei, Hang Zhang, Yunju Jo, Jong-Sun Kang, Ki-Tae Ha, Jongkil Joo, Hyun Joo Lee, Dongryeol Ryu

{"title":"Gut microbiota-generated metabolites: missing puzzles to hosts' health, diseases, and aging.","authors":"Yan Zhang, Shibo Wei, Hang Zhang, Yunju Jo, Jong-Sun Kang, Ki-Tae Ha, Jongkil Joo, Hyun Joo Lee, Dongryeol Ryu","doi":"","DOIUrl":"","url":null,"abstract":"The gut microbiota, an intricate community of bacteria residing in the gastrointestinal system, assumes a pivotal role in various physiological processes. Beyond its function in food breakdown and nutrient absorption, gut microbiota exerts a profound influence on immune and metabolic modulation by producing diverse gut microbiota-generated metabolites (GMGMs). These small molecules hold potential to impact host health via multiple pathways, which exhibit remarkable diversity, and have gained increasing attention in recent studies. Here, we elucidate the intricate implications and significant impacts of four specific metabolites, Urolithin A (UA), equol, Trimethylamine N-oxide (TMAO), and imidazole propionate, in shaping human health. Meanwhile, we also look into the advanced research on GMGMs, which demonstrate promising curative effects and hold great potential for further clinical therapies. Notably, the emergence of positive outcomes from clinical trials involving GMGMs, typified by UA, emphasizes their promising prospects in the pursuit of improved health and longevity. Collectively, the multifaceted impacts of GMGMs present intriguing avenues for future research and therapeutic interventions. [BMB Reports 2024; 57(5): 207-215].","PeriodicalId":9010,"journal":{"name":"BMB Reports","volume":" ","pages":"207-215"},"PeriodicalIF":2.9,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11139682/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140852753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

p38 mitogen-activated protein kinase contributes to TNFα-induced endothelial tube formation of bone-marrow-derived mesenchymal stem cells by activating the JAK/STAT/TIE2 signaling axis. p38丝裂原活化蛋白激酶通过激活JAK/STAT/TIES2信号轴，参与TNFα诱导的骨髓间充质干细胞内皮管的形成。

IF 2.9 3区生物学

BMB Reports Pub Date : 2024-05-01

Sukjin Ou, Tae Yoon Kim, Euitaek Jung, Soon Young Shin

引用次数: 0

The contribution of the nervous system in the cancer progression. 神经系统在癌症进展中的作用。

IF 2.9 3区生物学

BMB Reports Pub Date : 2024-04-01

Hongryeol Park, Chan Hee Lee

{"title":"The contribution of the nervous system in the cancer progression.","authors":"Hongryeol Park, Chan Hee Lee","doi":"","DOIUrl":"","url":null,"abstract":"Cancer progression is driven by genetic mutations, environmental factors, and intricate interactions within the tumor microenvironment (TME). The TME comprises of diverse cell types, such as cancer cells, immune cells, stromal cells, and neuronal cells. These cells mutually influence each other through various factors, including cytokines, vascular perfusion, and matrix stiffness. In the initial or developmental stage of cancer, neurotrophic factors such as nerve growth factor, brain-derived neurotrophic factor, and glial cell line-derived neurotrophic factor are associated with poor prognosis of various cancers by communicating with cancer cells, immune cells, and peripheral nerves within the TME. Over the past decade, research has been conducted to prevent cancer growth by controlling the activation of neurotrophic factors within tumors, exhibiting a novel attemt in cancer treatment with promising results. More recently, research focusing on controlling cancer growth through regulation of the autonomic nervous system, including the sympathetic and parasympathetic nervous systems, has gained significant attention. Sympathetic signaling predominantly promotes tumor progression, while the role of parasympathetic signaling varies among different cancer types. Neurotransmitters released from these signalings can directly or indirectly affect tumor cells or immune cells within the TME. Additionally, sensory nerve significantly promotes cancer progression. In the advanced stage of cancer, cancer-associated cachexia occurs, characterized by tissue wasting and reduced quality of life. This process involves the pathways via brainstem growth and differentiation factor 15-glial cell line-derived neurotrophic factor receptor alpha-like signaling and hypothalamic proopiomelanocortin neurons. Our review highlights the critical role of neurotrophic factors as well as central nervous system on the progression of cancer, offering promising avenues for targeted therapeutic strategies. [BMB Reports 2024; 57(4): 167-175].","PeriodicalId":9010,"journal":{"name":"BMB Reports","volume":" ","pages":"167-175"},"PeriodicalIF":2.9,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11058356/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140206330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0