BMB Reports最新文献_第8页

miR-328-5p functions as a critical negative regulator in early endothelial inflammation and advanced atherosclerosis. miR-328-5p 在早期内皮炎症和晚期动脉粥样硬化中发挥关键负调控因子的作用。

IF 2.9 3区生物学

BMB Reports Pub Date : 2024-08-01

Yangxia Zhang, Yingke Li, Zhisheng Han, Qingyang Huo, Longkai Ji, Xuejia Liu, Han Li, Xinxing Zhu, Zhipeng Hao

{"title":"miR-328-5p functions as a critical negative regulator in early endothelial inflammation and advanced atherosclerosis.","authors":"Yangxia Zhang, Yingke Li, Zhisheng Han, Qingyang Huo, Longkai Ji, Xuejia Liu, Han Li, Xinxing Zhu, Zhipeng Hao","doi":"","DOIUrl":"","url":null,"abstract":"Early proatherogenic inflammation constitutes a significant risk factor for atherogenesis development. Despite this, the precise molecular mechanisms driving this pathological progression largely remain elusive. Our study unveils a pivotal role for the microRNA miR-328-5p in dampening endothelial inflammation by modulating the stability of JUNB (JunB proto-oncogene). Perturbation of miR-328-5p levels results in heightened monocyte adhesion to endothelial cells and enhanced transendothelial migration, while its overexpression mitigates these inflammatory processes. Furthermore, miR-328-5p hinders macrophage polarization toward the pro-inflammatory M1 phenotype, and exerts a negative influence on atherosclerotic plaque formation in vivo. By pinpointing JUNB as a direct miR-328-5p target, our research underscores the potential of miR-328-5p as a therapeutic target for inflammatory atherosclerosis. Reintroduction of JUNB effectively counteracts the anti-atherosclerotic effects of miR-328-5p, highlighting the promise of pharmacological miR-328-5p targeting in managing inflammatory atherosclerosis. [BMB Reports 2024; 57(8): 375-380].","PeriodicalId":9010,"journal":{"name":"BMB Reports","volume":" ","pages":"375-380"},"PeriodicalIF":2.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11362139/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141449591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ASCL1-mediated direct reprogramming: converting ventral midbrain astrocytes into dopaminergic neurons for Parkinson's disease therapy. ASCL1 介导的直接重编程：将腹侧中脑星形胶质细胞转化为治疗帕金森病的多巴胺能神经元。

IF 2.9 3区生物学

BMB Reports Pub Date : 2024-08-01

Sang Hui Yong, Sang-Mi Kim, Gyeong Woon Kong, Seung Hwan Ko, Eun-Hye Lee, Yohan Oh, Chang-Hwan Park

{"title":"ASCL1-mediated direct reprogramming: converting ventral midbrain astrocytes into dopaminergic neurons for Parkinson's disease therapy.","authors":"Sang Hui Yong, Sang-Mi Kim, Gyeong Woon Kong, Seung Hwan Ko, Eun-Hye Lee, Yohan Oh, Chang-Hwan Park","doi":"","DOIUrl":"","url":null,"abstract":"Parkinson's disease (PD), characterized by dopaminergic neuron degeneration in the substantia nigra, is caused by various genetic and environmental factors. Current treatment methods are medication and surgery; however, a primary therapy has not yet been proposed. In this study, we aimed to develop a new treatment for PD that induces direct reprogramming of dopaminergic neurons (iDAN). Achaete-scute family bHLH transcription factor 1 (ASCL1) is a primary factor that initiates and regulates central nervous system development and induces neurogenesis. In addition, it interacts with BRN2 and MYT1L, which are crucial transcription factors for the direct conversion of fibroblasts into neurons. Overexpression of ASCL1 along with the transcription factors NURR1 and LMX1A can directly reprogram iDANs. Using a retrovirus, GFP-tagged ASCL1 was overexpressed in astrocytes. One week of culture in iDAN convertsion medium reprogrammed the astrocytes into iDANs. After 7 days of differentiation, TH+/TUJ1+ cells emerged. After 2 weeks, the number of mature TH+/TUJ1+ dopaminergic neurons increased. Only ventral midbrain (VM) astrocytes exhibited these results, not cortical astrocytes. Thus, VM astrocytes can undergo direct iDAN reprogramming with ASCL1 alone, in the absence of transcription factors that stimulate dopaminergic neurons development. [BMB Reports 2024; 57(8): 363-368].","PeriodicalId":9010,"journal":{"name":"BMB Reports","volume":" ","pages":"363-368"},"PeriodicalIF":2.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11362138/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140847983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Challenges and innovations in hematopoietic stem cell transplantation: exploring bone marrow niches and new model systems. 造血干细胞移植的挑战与创新：探索骨髓龛位和新模型系统。

IF 2.9 3区生物学

BMB Reports Pub Date : 2024-08-01

Byung-Chul Lee

{"title":"Challenges and innovations in hematopoietic stem cell transplantation: exploring bone marrow niches and new model systems.","authors":"Byung-Chul Lee","doi":"","DOIUrl":"","url":null,"abstract":"Hematopoietic stem cell transplantation (HSCT) remains an indispensable therapeutic strategy for various hematological diseases. This review discusses the pivotal role of bone marrow (BM) niches in influencing the efficacy of HSCT and evaluates the current animal models, emphasizing their limitations and the need for alternative models. Traditional animal models, mainly murine xenograft, have provided significant insights, but due to species-specific differences, are often constrained from accurately mimicking human physiological responses. These limitations highlight the importance of developing alternative models that can more realistically replicate human hematopoiesis. Emerging models that include BM organoids and BM-on-a-chip microfluidic systems promise enhanced understanding of HSCT dynamics. These models aim to provide more accurate simulations of the human BM microenvironment, potentially leading to improved preclinical assessments and therapeutic outcomes. This review highlights the complexities of the BM niche, discusses the limitations of current models, and suggests directions for future research using advanced model systems. [BMB Reports 2024; 57(8): 352-362].","PeriodicalId":9010,"journal":{"name":"BMB Reports","volume":" ","pages":"352-362"},"PeriodicalIF":2.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11362137/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141449586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Downregulated CDK10 promotes cancer progression and radioresistance in lung cancer through activating the JNK/c-Jun signaling pathway. 下调的 CDK10 通过激活 JNK/c-Jun 信号通路促进肺癌的癌症进展和放射抗性。

IF 2.9 3区生物学